Cp4.1LG11g07650 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g07650
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionprotein NRT1/ PTR FAMILY 4.6-like
LocationCp4.1LG11: 6077497 .. 6079688 (+)
RNA-Seq ExpressionCp4.1LG11g07650
SyntenyCp4.1LG11g07650
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGTATAATTTGGATGGAGGAAAATGATAAATAAATAAATAATTTGGTGTGAAAAGACATGAGGGGAGAGTGTGTTGTTGACGACATAAAAGGGTTGATTTTTTTATTTTTTTATTTTCTTTTGGAATTGGGTGTAAAAGGGGTTGGTGATTCTCACGGTGGAAGCGAAGGCGGCAACGGCGGCATCGGCGGCGGTGGTGTTCGTGGGGCTGTACCTGGTGGCGGTGGGGGTGGGGGGGATAAAGGGGTCGTTGCCGGCGCATGGGGCGGAGCAGTTGGAGGAGGGGACGGCGGAGGGGAGGAAGGGGAGGTCGAGTTTCTTCAACTACTTTGTGTTTAGTTTGTCGTGTGGGGCGCTTGTGGCGGTGACGTTGGTGGTGTGGATGGAAGACAACTTGGGGTGGGAGTGGGGGTTTGGAATCTCAACCATCGCTATTTTGGTGTCTATTCCTTTGTTCCTTGCTGGTTCCTGTTTTTATCGTAATAAGATCCCCGCTGCTGCTCCTCTTACCACCATTCTCAAGGTAAAACTTTTTTTTCTTTTTTTTTCTTTTTTTTTATCTAAATTTTTGTTTATAAAAATGTATGTCCCACGTCACCTAATTTACTCGCCTTTTGGGGACGTCCAAGTAAATCCATGAGAGCTTATGCTCAAAGTCGACAATATCATACCAGTGTGAAAAGTCGTGTTCATCTAACCTCTATGAATTTAAAGTAATGGGTTCTTATCTAAGAGTCAAATTTTGTAAAAAATGATAAAAAGACTTTTAAAATTCTTAATTTTTTTTAAAAACTTTTAAAATTATGTCTATATGCTATTAAGGGTATTTTTGGAATTTCTTAAAAGATTCAAACGTTTGCTTTTTAAATTGAAGGGTATTATTAAAACTTTACAAAAATGAAAAATGAAGGGTATCGGTATTCAAATTTAAGACTAACATTTGTACTCTTAAATTTTTTATTAAAATTTTTATGTCATAAACAATTTCTTACCAAATTCAAAAAATTTAAGGGTATTATTAAAAATTTATGAAAATTGTTTTGAGGATCAGATATTAGTATTATTTTTTAAAATTTTGGAGTAACGTTCGAACGTTAGTACTCTTAAATTTTTTAAAAAAATTTATGTTATTAACAAAAAGATCATTAAAATTTGAAGATATTATTGAATCCGAAAGATTTTTATTTTTTAAAATTTAAGAGTATTATTGCATTCAACGATCATCTGGATGATTCAGGTATTGGTAGCGGCACCTTTGAACGGTAGAAGGAGAAACAAAACAGCATCAAACGCAGTAGCAAGCATGACAAGAAGCCCATCCACCACAACCCCAAAGCCAACCAAGGAGCCAACACACACCACACCACCACCAACCCAAACCCTCAAATTCCTAAACAAAGCAATACAAACCCCACCCTTCCACCCATCACTAAGCTGCACAACCCAACAACTTGAAGACGTCAAAATAGTGTTAAAACTCCTCCCAATCTTCGCATGCACTATAATCCTCAACTCCTGCCTCGCTCAACTCTCCACCTTCTCGGTCGAACAAGCCTCGACTATGAACACCAAAATCGGATCCATCAAACTCCCACCCGCTTCTCTCCCTATCTTCCCTGTACTCTTCATCATACTCCTCGCCCCACTCTACAACCATCTCATAATCCCCTTCGCTCGCACCCTCACCCGAACCGAATCCGGTATCACTCACCTCCAACGCATTGGCGTCGGCCTACTTCTCTCAATTGTCGCCATGGCCATCGCCGCCCTCATCGAGGTCAAGCGCAAGGCTATTGCGGACGCACACCCGGCCGCCCACCCGCTCCCCATCACGTTCCTTTGGATTGGCTTCCAGTACTTGTTTTTGGGGTCTGCTGATTTGTTCACGTTGGCGGGGTTGTTGGAGTTTTTCTTTACCGAGGCTCCTTCTACCATGAGGTCTTTGGCTACGGCTCTCTCTTGGGCTTCATTGGCGGTTGGCTACTATCTAAGCTCCGTTATTGTGTCCCTTGTCAACCATGTCACTGCCAAGTCTTCCCACTCGCCTTGGCTGTCTGGCCAAGACATCAATCACTATGATCTTCATAAGTTCTACTGGCTCATGTGTGCGCTTAGTGCATTGAATTTCTTGCATTTCCTTTTTTGGGCCATCAAGTATAAATATCGGTCCACCCTAGCGAGCAAGTGA

mRNA sequence

ATGACACATGAGGGGAGAGGGTTGGTGATTCTCACGGTGGAAGCGAAGGCGGCAACGGCGGCATCGGCGGCGGTGGTGTTCGTGGGGCTGTACCTGGTGGCGGTGGGGGTGGGGGGGATAAAGGGGTCGTTGCCGGCGCATGGGGCGGAGCAGTTGGAGGAGGGGACGGCGGAGGGGAGGAAGGGGAGGTCGAGTTTCTTCAACTACTTTGTGTTTAGTTTGTCGTGTGGGGCGCTTGTGGCGGTGACGTTGGTGGTGTGGATGGAAGACAACTTGGGGTGGGAGTGGGGGTTTGGAATCTCAACCATCGCTATTTTGGTGTCTATTCCTTTGTTCCTTGCTGGTTCCTGTTTTTATCGTAATAAGATCCCCGCTGCTGCTCCTCTTACCACCATTCTCAAGAGTATTATTGCATTCAACGATCATCTGGATGATTCAGCGGCACCTTTGAACGGTAGAAGGAGAAACAAAACAGCATCAAACGCAGTAGCAAGCATGACAAGAAGCCCATCCACCACAACCCCAAAGCCAACCAAGGAGCCAACACACACCACACCACCACCAACCCAAACCCTCAAATTCCTAAACAAAGCAATACAAACCCCACCCTTCCACCCATCACTAAGCTGCACAACCCAACAACTTGAAGACGTCAAAATAGTGTTAAAACTCCTCCCAATCTTCGCATGCACTATAATCCTCAACTCCTGCCTCGCTCAACTCTCCACCTTCTCGGTCGAACAAGCCTCGACTATGAACACCAAAATCGGATCCATCAAACTCCCACCCGCTTCTCTCCCTATCTTCCCTGTACTCTTCATCATACTCCTCGCCCCACTCTACAACCATCTCATAATCCCCTTCGCTCGCACCCTCACCCGAACCGAATCCGGTATCACTCACCTCCAACGCATTGGCGTCGGCCTACTTCTCTCAATTGTCGCCATGGCCATCGCCGCCCTCATCGAGGTCAAGCGCAAGGCTATTGCGGACGCACACCCGGCCGCCCACCCGCTCCCCATCACGTTCCTTTGGATTGGCTTCCAGTACTTGTTTTTGGGGTCTGCTGATTTGTTCACGTTGGCGGGGTTGTTGGAGTTTTTCTTTACCGAGGCTCCTTCTACCATGAGGTCTTTGGCTACGGCTCTCTCTTGGGCTTCATTGGCGGTTGGCTACTATCTAAGCTCCGTTATTGTGTCCCTTGTCAACCATGTCACTGCCAAGTCTTCCCACTCGCCTTGGCTGTCTGGCCAAGACATCAATCACTATGATCTTCATAAGTTCTACTGGCTCATGTGTGCGCTTAGTGCATTGAATTTCTTGCATTTCCTTTTTTGGGCCATCAAGTATAAATATCGGTCCACCCTAGCGAGCAAGTGA

Coding sequence (CDS)

ATGACACATGAGGGGAGAGGGTTGGTGATTCTCACGGTGGAAGCGAAGGCGGCAACGGCGGCATCGGCGGCGGTGGTGTTCGTGGGGCTGTACCTGGTGGCGGTGGGGGTGGGGGGGATAAAGGGGTCGTTGCCGGCGCATGGGGCGGAGCAGTTGGAGGAGGGGACGGCGGAGGGGAGGAAGGGGAGGTCGAGTTTCTTCAACTACTTTGTGTTTAGTTTGTCGTGTGGGGCGCTTGTGGCGGTGACGTTGGTGGTGTGGATGGAAGACAACTTGGGGTGGGAGTGGGGGTTTGGAATCTCAACCATCGCTATTTTGGTGTCTATTCCTTTGTTCCTTGCTGGTTCCTGTTTTTATCGTAATAAGATCCCCGCTGCTGCTCCTCTTACCACCATTCTCAAGAGTATTATTGCATTCAACGATCATCTGGATGATTCAGCGGCACCTTTGAACGGTAGAAGGAGAAACAAAACAGCATCAAACGCAGTAGCAAGCATGACAAGAAGCCCATCCACCACAACCCCAAAGCCAACCAAGGAGCCAACACACACCACACCACCACCAACCCAAACCCTCAAATTCCTAAACAAAGCAATACAAACCCCACCCTTCCACCCATCACTAAGCTGCACAACCCAACAACTTGAAGACGTCAAAATAGTGTTAAAACTCCTCCCAATCTTCGCATGCACTATAATCCTCAACTCCTGCCTCGCTCAACTCTCCACCTTCTCGGTCGAACAAGCCTCGACTATGAACACCAAAATCGGATCCATCAAACTCCCACCCGCTTCTCTCCCTATCTTCCCTGTACTCTTCATCATACTCCTCGCCCCACTCTACAACCATCTCATAATCCCCTTCGCTCGCACCCTCACCCGAACCGAATCCGGTATCACTCACCTCCAACGCATTGGCGTCGGCCTACTTCTCTCAATTGTCGCCATGGCCATCGCCGCCCTCATCGAGGTCAAGCGCAAGGCTATTGCGGACGCACACCCGGCCGCCCACCCGCTCCCCATCACGTTCCTTTGGATTGGCTTCCAGTACTTGTTTTTGGGGTCTGCTGATTTGTTCACGTTGGCGGGGTTGTTGGAGTTTTTCTTTACCGAGGCTCCTTCTACCATGAGGTCTTTGGCTACGGCTCTCTCTTGGGCTTCATTGGCGGTTGGCTACTATCTAAGCTCCGTTATTGTGTCCCTTGTCAACCATGTCACTGCCAAGTCTTCCCACTCGCCTTGGCTGTCTGGCCAAGACATCAATCACTATGATCTTCATAAGTTCTACTGGCTCATGTGTGCGCTTAGTGCATTGAATTTCTTGCATTTCCTTTTTTGGGCCATCAAGTATAAATATCGGTCCACCCTAGCGAGCAAGTGA

Protein sequence

MTHEGRGLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK
Homology
BLAST of Cp4.1LG11g07650 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 547.7 bits (1410), Expect = 1.2e-154
Identity = 302/484 (62.40%), Postives = 365/484 (75.41%), Query Frame = 0

Query: 7   GLVILTVEAKA---------------ATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQ 66
           GL+ILT++A+                 + + AA++FVGLYLVA+GVGGIKGSL +HGAEQ
Sbjct: 110 GLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQ 169

Query: 67  LEEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPL 126
            +E T +GRK RS+FFNYFVF L+CGALVAVT VVW+EDN GWEWGFG+STIAI VSI +
Sbjct: 170 FDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILI 229

Query: 127 FLAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPS 186
           FL+GS FYRNKIP  +PLTTILK ++A +     S           ++SNAVASM+ SPS
Sbjct: 230 FLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG----------SSSNAVASMSVSPS 289

Query: 187 T--------------TTPKPTKEPTHTTPPP---TQTLKFLNKAIQTPPFHPSLSCTTQQ 246
                             KP +E     PP    T +LK LN A    P H  L CT QQ
Sbjct: 290 NHCVSKGKKEVESQGELEKPRQE--EALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQ 349

Query: 247 LEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFI 306
           +EDVKIVLK+LPIFACTI+LN CLAQLSTFSV+QA++MNTKIGS+K+PPASLPIFPV+FI
Sbjct: 350 VEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFI 409

Query: 307 ILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAH- 366
           ++LAP+Y+HLIIPFAR  T+TE+G+THLQRIGVGL+LSI+AMA+AAL+E+KRK +A    
Sbjct: 410 MILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSG 469

Query: 367 --PAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVG 426
              +   LP+TFLWI  QYLFLGSADLFTLAGLLE+FFTEAPS+MRSLAT+LSWASLA+G
Sbjct: 470 LLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMG 529

Query: 427 YYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYK 456
           YYLSSVIVS+VN +T  S ++PWL G+ IN Y L  FYWLMC LSA NFLH+LFWA++YK
Sbjct: 530 YYLSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYK 581

BLAST of Cp4.1LG11g07650 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 5.5e-139
Identity = 278/472 (58.90%), Postives = 343/472 (72.67%), Query Frame = 0

Query: 7   GLVILTVEAKAAT------------------AASAAVVFVGLYLVAVGVGGIKGSLPAHG 66
           GL++LT++A+  +                   + AA +FVGLYLV++G+GGIKGSLP+HG
Sbjct: 112 GLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHG 171

Query: 67  AEQLEEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVS 126
           AEQ +EGT +GRK RS+FFNY+VF LSCGALVAVT VVW+EDN GWEWGFG+STI+I +S
Sbjct: 172 AEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLS 231

Query: 127 IPLFLAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTR 186
           I +FL GS FY+NKIP  +PLTTI K ++A                    +  + +S T 
Sbjct: 232 ILVFLLGSRFYKNKIPRGSPLTTIFKVLLA-------------------ASIVSCSSKTS 291

Query: 187 SPSTTTPKPTKEPTHTTPPP--TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLP 246
           S   T+ +   E    TP    T +L  LNKAI+    H  L CT QQ+EDVKIVLK+LP
Sbjct: 292 SNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLP 351

Query: 247 IFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLII 306
           IF CTI+LN CLAQLST+SV QA+TMN KI +  +P ASLP+FPV+F+++LAP Y+HLII
Sbjct: 352 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 411

Query: 307 PFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAI---ADAHPAAHPLPITF 366
           PFAR +T++E GITHLQRIGVGL+LSIVAMA+AAL+E+KRK +   A    +   LPITF
Sbjct: 412 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 471

Query: 367 LWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVN 426
           LWI  QYLFLGSADLFTLAGLLEFFFTEAPS+MRSLAT+LSWASLA+GYYLSSV+V +VN
Sbjct: 472 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 531

Query: 427 HVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRST 456
            VT  +  SPWL G+ +N   L  FYWLMC LS +NFLH+LFWA +YKY ST
Sbjct: 532 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Cp4.1LG11g07650 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 484.6 bits (1246), Expect = 1.3e-135
Identity = 264/459 (57.52%), Postives = 342/459 (74.51%), Query Frame = 0

Query: 7   GLVILTVEAKAATAA--SAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRS 66
           GL++LTV+A   +    S   +FVGLYLVA+GVGGIKGSLP HGAEQ +E T+ GR+ RS
Sbjct: 141 GLMVLTVQAHEHSTEPWSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRS 200

Query: 67  SFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIP 126
            FFNYF+FSLSCGAL+AVT+VVW+EDN GW +GFG+ST AIL+S+P+FLAGS  YR K+P
Sbjct: 201 FFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVP 260

Query: 127 AAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHT 186
           + +P+TT+ K +         +AA     ++ +T+   V   TR+    +   TK+  + 
Sbjct: 261 SGSPITTLFKVL---------TAALYAKYKKRRTSRIVVTCHTRNDCDDS--VTKQ--NC 320

Query: 187 TPPPTQTLKFLNKAIQTPPFHP-SLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLST 246
                    FL + ++     P  L CT +Q++DVKIV+K+LPIF  TI+LN CLAQLST
Sbjct: 321 DGDDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLST 380

Query: 247 FSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQ 306
           FSV+QASTMNTK+GS  +PPA+LP+FPV+F+++LAP YNHL++P AR  T+TE+GITHLQ
Sbjct: 381 FSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQ 440

Query: 307 RIGVGLLLSIVAMAIAALIEVKRKAIA--------DAHPAAHPLPITFLWIGFQYLFLGS 366
           RIG GL+LSIVAMA+AAL+E KRK +          +  ++ PLPITFLW+  QY+FLGS
Sbjct: 441 RIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGS 500

Query: 367 ADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWL 426
           ADLFTLAG++EFFFTEAPSTMRSLAT+LSWASLA+GYY SSV+VS VN VT  + H+PWL
Sbjct: 501 ADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWL 560

Query: 427 SGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRS 455
            G+++N Y L +FYWLMC LS +NFLH+LFWA +Y YRS
Sbjct: 561 LGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRS 586

BLAST of Cp4.1LG11g07650 vs. ExPASy Swiss-Prot
Match: Q9LFB8 (Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 3.0e-68
Identity = 168/475 (35.37%), Postives = 273/475 (57.47%), Query Frame = 0

Query: 7   GLVILTVEAKA-------------ATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLE 66
           G+ +LT+ A               ATA   A+ F+ LYL+A+G GGIK  + + GA+Q +
Sbjct: 110 GMTLLTISASVPGLTPTCSGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFD 169

Query: 67  EGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFL 126
           +   + ++ +SSFFN+F F ++ GA++A +++VW++ N+GW WG G+ T+A+ +++  F 
Sbjct: 170 DTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFF 229

Query: 127 AGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASM--TRSPS 186
           AGS FYR + P  +PLT +L+ I+A               R++K       S+      +
Sbjct: 230 AGSNFYRLQKPGGSPLTRMLQVIVA-------------SCRKSKVKIPEDESLLYENQDA 289

Query: 187 TTTPKPTKEPTHTTPPPTQTLKFLNK-AIQTPPFHPSLS-------CTTQQLEDVKIVLK 246
            ++   +++  H     T+ L F +K A++T   +   +       CT  Q+E++K +++
Sbjct: 290 ESSIIGSRKLEH-----TKILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIR 349

Query: 247 LLPIFACTIILNSCLAQLSTFSVEQASTMNTKIG-SIKLPPASLPIFPVLFIILLAPLYN 306
           LLPI+A  I+  S  +Q+ T  V Q +T++  +G + K+P ASL +F  L ++  AP+Y+
Sbjct: 350 LLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYD 409

Query: 307 HLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPA--AHPLP 366
            LI+PFAR  T  E G T LQRIG+GL++SI +M  A ++EV R      H       +P
Sbjct: 410 KLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIP 469

Query: 367 ITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVS 426
           +T  W   QY  +G A++FT  G LEFF+ +AP  MRSL +ALS  ++A G YLS+ +V+
Sbjct: 470 MTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVT 529

Query: 427 LVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRST 456
           LV  VT       W++ +++N+  L  F+WL+  LS LNFL +L+ A  Y Y+ T
Sbjct: 530 LVTKVTRSGGRPGWIA-KNLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYKKT 565

BLAST of Cp4.1LG11g07650 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 260.0 bits (663), Expect = 5.1e-68
Identity = 161/448 (35.94%), Postives = 262/448 (58.48%), Query Frame = 0

Query: 20  AASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSFFNYFVFSLSCGAL 79
           ++  AV FV LY++A+G GGIK  + + GA+Q +E     +  +SSFFN+F FS++ GAL
Sbjct: 136 SSQTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKSSFFNWFYFSINVGAL 195

Query: 80  VAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAAAPLTTILKSIIAF 139
           +A T++VW++ N+GW WGFG+ T+A+++++  F  GS FYR + P  +PLT I + I+A 
Sbjct: 196 IAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRPGGSPLTRIFQVIVAA 255

Query: 140 NDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTPPPTQTLKFLNKA- 199
              +             K   +       +   +  K +++  H     T  LKF +KA 
Sbjct: 256 FRKIS-----------VKVPEDKSLLFETADDESNIKGSRKLVH-----TDNLKFFDKAA 315

Query: 200 -------IQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQAST 259
                  I+    +P   C+  Q+E++K ++ LLP++A  I+  +  +Q+ST  V Q +T
Sbjct: 316 VESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNT 375

Query: 260 MNTKIG-SIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLL 319
           M+  +G + ++P ASL +F  + ++   P+Y+  IIP AR  TR E G T LQR+G+GL+
Sbjct: 376 MDQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLV 435

Query: 320 LSIVAMAIAALIEVKRKAIADAHPA--AHPLPITFLWIGFQYLFLGSADLFTLAGLLEFF 379
           +SI AM  A ++EV R      H A     + ++  W   QYL +G A++FT  G LEFF
Sbjct: 436 VSIFAMITAGVLEVVRLDYVKTHNAYDQKQIHMSIFWQIPQYLLIGCAEVFTFIGQLEFF 495

Query: 380 FTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLHKF 439
           + +AP  MRSL +ALS  ++A+G YLS+V+V++V  +T K+    W+   ++N   L  F
Sbjct: 496 YDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGKPGWIP-DNLNRGHLDYF 555

Query: 440 YWLMCALSALNFLHFLFWAIKYKYRSTL 457
           ++L+  LS LNFL +L+ + +YKY+  +
Sbjct: 556 FYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Cp4.1LG11g07650 vs. NCBI nr
Match: XP_023546035.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828 bits (2139), Expect = 1.15e-299
Identity = 441/453 (97.35%), Postives = 443/453 (97.79%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF 66
           GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF
Sbjct: 113 GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF 172

Query: 67  FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA 126
           FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA
Sbjct: 173 FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA 232

Query: 127 APLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 186
           APLTTILK ++A         APLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP
Sbjct: 233 APLTTILKVLVA---------APLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 292

Query: 187 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 246
           PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV
Sbjct: 293 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 352

Query: 247 EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 306
           EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG
Sbjct: 353 EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 412

Query: 307 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 366
           VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE
Sbjct: 413 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 472

Query: 367 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH 426
           FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH
Sbjct: 473 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH 532

Query: 427 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK
Sbjct: 533 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Cp4.1LG11g07650 vs. NCBI nr
Match: XP_022946066.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata])

HSP 1 Score: 810 bits (2091), Expect = 2.33e-292
Identity = 430/453 (94.92%), Postives = 436/453 (96.25%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF 66
           GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLP HGAEQ EEGTAEGR+GRSSF
Sbjct: 113 GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSF 172

Query: 67  FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA 126
           FNYFVFSLSCGA+VAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPA 
Sbjct: 173 FNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAG 232

Query: 127 APLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 186
           APLTTILK ++A         A LNGR RNKTASNAVASMTRSPSTTTPKPTKEPTHTTP
Sbjct: 233 APLTTILKVLVA---------ATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 292

Query: 187 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 246
           PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVK+VLKLLPIFACTIILNSCLAQLSTFSV
Sbjct: 293 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSV 352

Query: 247 EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 306
           EQASTMNTKIGSIKLPPASLPIFP+LFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG
Sbjct: 353 EQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 412

Query: 307 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 366
           VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE
Sbjct: 413 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 472

Query: 367 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH 426
           FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVN VTAKSSHSPWLS QDINHYDLH
Sbjct: 473 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLH 532

Query: 427 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK
Sbjct: 533 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Cp4.1LG11g07650 vs. NCBI nr
Match: XP_022999670.1 (protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima])

HSP 1 Score: 783 bits (2022), Expect = 7.42e-282
Identity = 415/453 (91.61%), Postives = 429/453 (94.70%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF 66
           GLVILTVEAKAA+AASAA VFVGLYLVAVGVGGIKGSL AHGAEQ EEGTAEGR+GRSSF
Sbjct: 114 GLVILTVEAKAASAASAAAVFVGLYLVAVGVGGIKGSLAAHGAEQFEEGTAEGRRGRSSF 173

Query: 67  FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA 126
           FNYFVFSLSCGALV VTLVVW+EDNLGWEWGFGISTIAILVSIPLFL+GSCFYRNKIP A
Sbjct: 174 FNYFVFSLSCGALVGVTLVVWIEDNLGWEWGFGISTIAILVSIPLFLSGSCFYRNKIPTA 233

Query: 127 APLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 186
           APLTTILK ++A         A LNGRRRNKTA+NAVASMTRSPSTTTPKPTKEPTHTTP
Sbjct: 234 APLTTILKVLVA---------ATLNGRRRNKTATNAVASMTRSPSTTTPKPTKEPTHTTP 293

Query: 187 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 246
           P TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV
Sbjct: 294 P-TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 353

Query: 247 EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 306
           EQASTMNT+IGSIK PPASLPIFPV+FII+LAPLYNHLI+PFARTLTRTESGITHLQRIG
Sbjct: 354 EQASTMNTQIGSIKFPPASLPIFPVIFIIILAPLYNHLIVPFARTLTRTESGITHLQRIG 413

Query: 307 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 366
           VGLLLSIVAM IAALIEVKRKA+ADAHPAAHPLPITFLWI FQYLFLGSADLFTLAGLLE
Sbjct: 414 VGLLLSIVAMVIAALIEVKRKAVADAHPAAHPLPITFLWIAFQYLFLGSADLFTLAGLLE 473

Query: 367 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH 426
           FFFTEAP TMRSLATALSWASLAVGYYLSSVIVSLVN VTAKSSHSPWLSGQDINHYDLH
Sbjct: 474 FFFTEAPPTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSGQDINHYDLH 533

Query: 427 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           KFYWLMCALSALNFLHFLFWA+KYKYRST+  K
Sbjct: 534 KFYWLMCALSALNFLHFLFWALKYKYRSTIERK 556

BLAST of Cp4.1LG11g07650 vs. NCBI nr
Match: XP_038886228.1 (protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida])

HSP 1 Score: 667 bits (1721), Expect = 8.19e-236
Identity = 358/470 (76.17%), Postives = 402/470 (85.53%), Query Frame = 0

Query: 7   GLVILTVEAKAATAAS--------------AAVVFVGLYLVAVGVGGIKGSLPAHGAEQL 66
           GLV+LTV+AK +++                AAV+F+GLYLVA+GVGGIKGSLPAHGAEQ 
Sbjct: 113 GLVMLTVQAKVSSSCEEGNSKGCKEVGGREAAVLFIGLYLVALGVGGIKGSLPAHGAEQF 172

Query: 67  EEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLF 126
           +EG+ +GRK RSSFFNYFVFSLSCGAL+AVT VVW+EDNLGW+WGFGISTI+I +SIPLF
Sbjct: 173 DEGSPQGRKRRSSFFNYFVFSLSCGALIAVTFVVWVEDNLGWQWGFGISTISIFLSIPLF 232

Query: 127 LAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPST 186
           LAGS FYRNKIP  +PLTTILK ++A         A LN    NK A+NAVAS+ RSPST
Sbjct: 233 LAGSPFYRNKIPTGSPLTTILKVLVA---------AALNRGSSNKPATNAVASLARSPST 292

Query: 187 TTPKP--TKEPT-HTTPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFA 246
           +T K    KE T + T  PTQ+LKFLNKA+Q PPFHPSL CTTQQLEDVKIVLK+LPIFA
Sbjct: 293 STAKQLINKELTQNNTTNPTQSLKFLNKAVQNPPFHPSLKCTTQQLEDVKIVLKVLPIFA 352

Query: 247 CTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFA 306
           CTIILNSCLAQLSTFS+EQASTMNTKI S+K+PPASLPIFPVLFIILLAPLY+HLIIPFA
Sbjct: 353 CTIILNSCLAQLSTFSIEQASTMNTKISSLKVPPASLPIFPVLFIILLAPLYDHLIIPFA 412

Query: 307 RTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQ 366
           RTLTRTE+GITHLQRIGVGLLLS++AMA+AAL+E KRKA+A AHP AHPLPITFLWI FQ
Sbjct: 413 RTLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAVAGAHPDAHPLPITFLWIAFQ 472

Query: 367 YLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKS 426
           YLFLGSADLFTLAGLLEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VN VTAK+
Sbjct: 473 YLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNRVTAKA 532

Query: 427 SHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           SH+PWLSG +INHY L  FYWLMCALS  NFLH+LFWA+KYKYRST  +K
Sbjct: 533 SHTPWLSGHNINHYRLSNFYWLMCALSGFNFLHYLFWALKYKYRSTQGNK 573

BLAST of Cp4.1LG11g07650 vs. NCBI nr
Match: XP_008456458.1 (PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa] >TYK00047.1 protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 647 bits (1670), Expect = 4.75e-228
Identity = 348/471 (73.89%), Postives = 398/471 (84.50%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASA-------------AVVFVGLYLVAVGVGGIKGSLPAHGAEQLE 66
           GLV+LTV+AK +++  A             AV+F+GLYLVA+GVGGIKGSLPAHGAEQ +
Sbjct: 113 GLVMLTVQAKVSSSCEAGTKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFD 172

Query: 67  EGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFL 126
           E T +GRK RSSFFNYFVFSLSCGALVAVT VVW+EDNLGW+WGFGISTI+  +SIPLF 
Sbjct: 173 ESTTQGRKRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFF 232

Query: 127 AGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTT 186
           AGS FYRNKIP  +PLTTILK ++A         A LN R   K A+NAVAS+ RSPSTT
Sbjct: 233 AGSPFYRNKIPTGSPLTTILKVLVA---------ATLNRRSNKKPATNAVASLARSPSTT 292

Query: 187 -TPKP--TKEPTHT-TPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFA 246
            TPKP   K+ T + T  PTQ+LKFLNKA+Q PPFHPS+ CTTQQLEDVKIV+K+LPIFA
Sbjct: 293 STPKPLINKDLTESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFA 352

Query: 247 CTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFA 306
           CTIILNSCLAQLSTFS+EQA+TMNTKI S+K+PPASLP+FPVLFII+LAPLY+HLIIPFA
Sbjct: 353 CTIILNSCLAQLSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFA 412

Query: 307 RTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGF 366
           R+LTRTE+GITHLQRIGVGLLLS++AMA+AAL+E KRKA+ADA+P    PLPITFLWI F
Sbjct: 413 RSLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAF 472

Query: 367 QYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAK 426
           QYLFLGSADLFTLAGLLEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VNHV+AK
Sbjct: 473 QYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAK 532

Query: 427 SSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           + H PWLSG +INHY L  FYW+MC LS  NFLH+LFWA+KYKYRST  +K
Sbjct: 533 AWHRPWLSGHNINHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Cp4.1LG11g07650 vs. ExPASy TrEMBL
Match: A0A6J1G2M6 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 PE=3 SV=1)

HSP 1 Score: 810 bits (2091), Expect = 1.13e-292
Identity = 430/453 (94.92%), Postives = 436/453 (96.25%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF 66
           GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLP HGAEQ EEGTAEGR+GRSSF
Sbjct: 113 GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPTHGAEQFEEGTAEGRRGRSSF 172

Query: 67  FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA 126
           FNYFVFSLSCGA+VAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPA 
Sbjct: 173 FNYFVFSLSCGAVVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAG 232

Query: 127 APLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 186
           APLTTILK ++A         A LNGR RNKTASNAVASMTRSPSTTTPKPTKEPTHTTP
Sbjct: 233 APLTTILKVLVA---------ATLNGRSRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 292

Query: 187 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 246
           PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVK+VLKLLPIFACTIILNSCLAQLSTFSV
Sbjct: 293 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKVVLKLLPIFACTIILNSCLAQLSTFSV 352

Query: 247 EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 306
           EQASTMNTKIGSIKLPPASLPIFP+LFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG
Sbjct: 353 EQASTMNTKIGSIKLPPASLPIFPILFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 412

Query: 307 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 366
           VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE
Sbjct: 413 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 472

Query: 367 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH 426
           FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVN VTAKSSHSPWLS QDINHYDLH
Sbjct: 473 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSAQDINHYDLH 532

Query: 427 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK
Sbjct: 533 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 556

BLAST of Cp4.1LG11g07650 vs. ExPASy TrEMBL
Match: A0A6J1KG66 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111493955 PE=3 SV=1)

HSP 1 Score: 783 bits (2022), Expect = 3.59e-282
Identity = 415/453 (91.61%), Postives = 429/453 (94.70%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSF 66
           GLVILTVEAKAA+AASAA VFVGLYLVAVGVGGIKGSL AHGAEQ EEGTAEGR+GRSSF
Sbjct: 114 GLVILTVEAKAASAASAAAVFVGLYLVAVGVGGIKGSLAAHGAEQFEEGTAEGRRGRSSF 173

Query: 67  FNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAA 126
           FNYFVFSLSCGALV VTLVVW+EDNLGWEWGFGISTIAILVSIPLFL+GSCFYRNKIP A
Sbjct: 174 FNYFVFSLSCGALVGVTLVVWIEDNLGWEWGFGISTIAILVSIPLFLSGSCFYRNKIPTA 233

Query: 127 APLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHTTP 186
           APLTTILK ++A         A LNGRRRNKTA+NAVASMTRSPSTTTPKPTKEPTHTTP
Sbjct: 234 APLTTILKVLVA---------ATLNGRRRNKTATNAVASMTRSPSTTTPKPTKEPTHTTP 293

Query: 187 PPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 246
           P TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV
Sbjct: 294 P-TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSV 353

Query: 247 EQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIG 306
           EQASTMNT+IGSIK PPASLPIFPV+FII+LAPLYNHLI+PFARTLTRTESGITHLQRIG
Sbjct: 354 EQASTMNTQIGSIKFPPASLPIFPVIFIIILAPLYNHLIVPFARTLTRTESGITHLQRIG 413

Query: 307 VGLLLSIVAMAIAALIEVKRKAIADAHPAAHPLPITFLWIGFQYLFLGSADLFTLAGLLE 366
           VGLLLSIVAM IAALIEVKRKA+ADAHPAAHPLPITFLWI FQYLFLGSADLFTLAGLLE
Sbjct: 414 VGLLLSIVAMVIAALIEVKRKAVADAHPAAHPLPITFLWIAFQYLFLGSADLFTLAGLLE 473

Query: 367 FFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLH 426
           FFFTEAP TMRSLATALSWASLAVGYYLSSVIVSLVN VTAKSSHSPWLSGQDINHYDLH
Sbjct: 474 FFFTEAPPTMRSLATALSWASLAVGYYLSSVIVSLVNRVTAKSSHSPWLSGQDINHYDLH 533

Query: 427 KFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           KFYWLMCALSALNFLHFLFWA+KYKYRST+  K
Sbjct: 534 KFYWLMCALSALNFLHFLFWALKYKYRSTIERK 556

BLAST of Cp4.1LG11g07650 vs. ExPASy TrEMBL
Match: A0A5D3BLL0 (Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00950 PE=3 SV=1)

HSP 1 Score: 647 bits (1670), Expect = 2.30e-228
Identity = 348/471 (73.89%), Postives = 398/471 (84.50%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASA-------------AVVFVGLYLVAVGVGGIKGSLPAHGAEQLE 66
           GLV+LTV+AK +++  A             AV+F+GLYLVA+GVGGIKGSLPAHGAEQ +
Sbjct: 113 GLVMLTVQAKVSSSCEAGTKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFD 172

Query: 67  EGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFL 126
           E T +GRK RSSFFNYFVFSLSCGALVAVT VVW+EDNLGW+WGFGISTI+  +SIPLF 
Sbjct: 173 ESTTQGRKRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFF 232

Query: 127 AGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTT 186
           AGS FYRNKIP  +PLTTILK ++A         A LN R   K A+NAVAS+ RSPSTT
Sbjct: 233 AGSPFYRNKIPTGSPLTTILKVLVA---------ATLNRRSNKKPATNAVASLARSPSTT 292

Query: 187 -TPKP--TKEPTHT-TPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFA 246
            TPKP   K+ T + T  PTQ+LKFLNKA+Q PPFHPS+ CTTQQLEDVKIV+K+LPIFA
Sbjct: 293 STPKPLINKDLTESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFA 352

Query: 247 CTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFA 306
           CTIILNSCLAQLSTFS+EQA+TMNTKI S+K+PPASLP+FPVLFII+LAPLY+HLIIPFA
Sbjct: 353 CTIILNSCLAQLSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFA 412

Query: 307 RTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGF 366
           R+LTRTE+GITHLQRIGVGLLLS++AMA+AAL+E KRKA+ADA+P    PLPITFLWI F
Sbjct: 413 RSLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAF 472

Query: 367 QYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAK 426
           QYLFLGSADLFTLAGLLEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VNHV+AK
Sbjct: 473 QYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAK 532

Query: 427 SSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           + H PWLSG +INHY L  FYW+MC LS  NFLH+LFWA+KYKYRST  +K
Sbjct: 533 AWHRPWLSGHNINHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Cp4.1LG11g07650 vs. ExPASy TrEMBL
Match: A0A1S3C3A1 (protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 SV=1)

HSP 1 Score: 647 bits (1670), Expect = 2.30e-228
Identity = 348/471 (73.89%), Postives = 398/471 (84.50%), Query Frame = 0

Query: 7   GLVILTVEAKAATAASA-------------AVVFVGLYLVAVGVGGIKGSLPAHGAEQLE 66
           GLV+LTV+AK +++  A             AV+F+GLYLVA+GVGGIKGSLPAHGAEQ +
Sbjct: 113 GLVMLTVQAKVSSSCEAGTKGCKEVGGKEVAVLFIGLYLVALGVGGIKGSLPAHGAEQFD 172

Query: 67  EGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFL 126
           E T +GRK RSSFFNYFVFSLSCGALVAVT VVW+EDNLGW+WGFGISTI+  +SIPLF 
Sbjct: 173 ESTTQGRKRRSSFFNYFVFSLSCGALVAVTFVVWVEDNLGWQWGFGISTISTFLSIPLFF 232

Query: 127 AGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTT 186
           AGS FYRNKIP  +PLTTILK ++A         A LN R   K A+NAVAS+ RSPSTT
Sbjct: 233 AGSPFYRNKIPTGSPLTTILKVLVA---------ATLNRRSNKKPATNAVASLARSPSTT 292

Query: 187 -TPKP--TKEPTHT-TPPPTQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFA 246
            TPKP   K+ T + T  PTQ+LKFLNKA+Q PPFHPS+ CTTQQLEDVKIV+K+LPIFA
Sbjct: 293 STPKPLINKDLTESNTTTPTQSLKFLNKAVQNPPFHPSVKCTTQQLEDVKIVIKVLPIFA 352

Query: 247 CTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFA 306
           CTIILNSCLAQLSTFS+EQA+TMNTKI S+K+PPASLP+FPVLFII+LAPLY+HLIIPFA
Sbjct: 353 CTIILNSCLAQLSTFSIEQAATMNTKISSLKIPPASLPVFPVLFIIVLAPLYDHLIIPFA 412

Query: 307 RTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPAAH-PLPITFLWIGF 366
           R+LTRTE+GITHLQRIGVGLLLS++AMA+AAL+E KRKA+ADA+P    PLPITFLWI F
Sbjct: 413 RSLTRTEAGITHLQRIGVGLLLSVLAMAVAALVETKRKAVADANPDVQGPLPITFLWIAF 472

Query: 367 QYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAK 426
           QYLFLGSADLFTLAGLLEFFFTEAP+TMRSLATALSWASLAVGYYLSSVIVS+VNHV+AK
Sbjct: 473 QYLFLGSADLFTLAGLLEFFFTEAPATMRSLATALSWASLAVGYYLSSVIVSVVNHVSAK 532

Query: 427 SSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRSTLASK 459
           + H PWLSG +INHY L  FYW+MC LS  NFLH+LFWA+KYKYRST  +K
Sbjct: 533 AWHRPWLSGHNINHYRLSNFYWVMCVLSGFNFLHYLFWALKYKYRSTQGNK 574

BLAST of Cp4.1LG11g07650 vs. ExPASy TrEMBL
Match: A0A0A0KHD2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G404120 PE=3 SV=1)

HSP 1 Score: 643 bits (1658), Expect = 2.58e-228
Identity = 342/443 (77.20%), Postives = 386/443 (87.13%), Query Frame = 0

Query: 24  AVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRSSFFNYFVFSLSCGALVAVT 83
           AV+F+GLYLVA+GVGGIKGSLPAHGAEQ +E T +GRK RSSFFNYFVFSLSCGAL+AVT
Sbjct: 29  AVLFIGLYLVALGVGGIKGSLPAHGAEQFDESTPQGRKRRSSFFNYFVFSLSCGALIAVT 88

Query: 84  LVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIPAAAPLTTILKSIIAFNDHL 143
            VVW+EDNLGW+WGFGISTI+I +SIPLF AGS FYRNKIP  +PLTTILK ++A     
Sbjct: 89  FVVWIEDNLGWQWGFGISTISIFLSIPLFFAGSPFYRNKIPTGSPLTTILKVLVA----- 148

Query: 144 DDSAAPLNGRRRNKTASNAVASMTRSPSTT-TPKP--TKEPTH---TTPPPTQTLKFLNK 203
               A LN R   K A+NAVAS+ RSPSTT TPK    K+ T    TTP  TQ+LKFLNK
Sbjct: 149 ----ATLNRRSNKKPATNAVASLARSPSTTSTPKQLINKDLTENNVTTP--TQSLKFLNK 208

Query: 204 AIQTPPFHPSLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIG 263
           A+Q PPFHPSL CTTQQLEDVKIV+K+LPIFACTIILNSCLAQLSTFS+EQA+TMNTKI 
Sbjct: 209 AVQNPPFHPSLKCTTQQLEDVKIVIKVLPIFACTIILNSCLAQLSTFSIEQAATMNTKIY 268

Query: 264 SIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMA 323
           S+K+PPASLP+FPVLFII+LAPLY+HLIIPFAR+LTRTE+GITHLQRIGVGLLLS++AMA
Sbjct: 269 SLKIPPASLPVFPVLFIIILAPLYDHLIIPFARSLTRTEAGITHLQRIGVGLLLSVLAMA 328

Query: 324 IAALIEVKRKAIADAHPAAH-PLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTM 383
           +AAL+E KRKA+ADA+P  H PLPITFLWI FQYLFLGSADLFTLAGLLEFFFTEAP+TM
Sbjct: 329 VAALVETKRKAVADANPDVHGPLPITFLWIAFQYLFLGSADLFTLAGLLEFFFTEAPTTM 388

Query: 384 RSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALS 443
           RSLATALSWASLAVGYYLSSVIVS+VNHV+AK+ H PWLSG +INHY L  FYW+MC LS
Sbjct: 389 RSLATALSWASLAVGYYLSSVIVSVVNHVSAKAWHRPWLSGHNINHYRLSNFYWVMCVLS 448

Query: 444 ALNFLHFLFWAIKYKYRSTLASK 459
            LNFLH+LFWA+KYKYRST  +K
Sbjct: 449 GLNFLHYLFWALKYKYRSTQGNK 460

BLAST of Cp4.1LG11g07650 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 547.7 bits (1410), Expect = 8.6e-156
Identity = 302/484 (62.40%), Postives = 365/484 (75.41%), Query Frame = 0

Query: 7   GLVILTVEAKA---------------ATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQ 66
           GL+ILT++A+                 + + AA++FVGLYLVA+GVGGIKGSL +HGAEQ
Sbjct: 110 GLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAMLFVGLYLVALGVGGIKGSLASHGAEQ 169

Query: 67  LEEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPL 126
            +E T +GRK RS+FFNYFVF L+CGALVAVT VVW+EDN GWEWGFG+STIAI VSI +
Sbjct: 170 FDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVWLEDNKGWEWGFGVSTIAIFVSILI 229

Query: 127 FLAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPS 186
           FL+GS FYRNKIP  +PLTTILK ++A +     S           ++SNAVASM+ SPS
Sbjct: 230 FLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG----------SSSNAVASMSVSPS 289

Query: 187 T--------------TTPKPTKEPTHTTPPP---TQTLKFLNKAIQTPPFHPSLSCTTQQ 246
                             KP +E     PP    T +LK LN A    P H  L CT QQ
Sbjct: 290 NHCVSKGKKEVESQGELEKPRQE--EALPPRAQLTNSLKVLNGAADEKPVHRLLECTVQQ 349

Query: 247 LEDVKIVLKLLPIFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFI 306
           +EDVKIVLK+LPIFACTI+LN CLAQLSTFSV+QA++MNTKIGS+K+PPASLPIFPV+FI
Sbjct: 350 VEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKIPPASLPIFPVVFI 409

Query: 307 ILLAPLYNHLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAH- 366
           ++LAP+Y+HLIIPFAR  T+TE+G+THLQRIGVGL+LSI+AMA+AAL+E+KRK +A    
Sbjct: 410 MILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAALVEIKRKGVAKDSG 469

Query: 367 --PAAHPLPITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVG 426
              +   LP+TFLWI  QYLFLGSADLFTLAGLLE+FFTEAPS+MRSLAT+LSWASLA+G
Sbjct: 470 LLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPSSMRSLATSLSWASLAMG 529

Query: 427 YYLSSVIVSLVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYK 456
           YYLSSVIVS+VN +T  S ++PWL G+ IN Y L  FYWLMC LSA NFLH+LFWA++YK
Sbjct: 530 YYLSSVIVSIVNSITGSSGNTPWLRGKSINRYKLDYFYWLMCVLSAANFLHYLFWAMRYK 581

BLAST of Cp4.1LG11g07650 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 495.7 bits (1275), Expect = 3.9e-140
Identity = 278/472 (58.90%), Postives = 343/472 (72.67%), Query Frame = 0

Query: 7   GLVILTVEAKAAT------------------AASAAVVFVGLYLVAVGVGGIKGSLPAHG 66
           GL++LT++A+  +                   + AA +FVGLYLV++G+GGIKGSLP+HG
Sbjct: 108 GLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHG 167

Query: 67  AEQLEEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVS 126
           AEQ +EGT +GRK RS+FFNY+VF LSCGALVAVT VVW+EDN GWEWGFG+STI+I +S
Sbjct: 168 AEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLS 227

Query: 127 IPLFLAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTR 186
           I +FL GS FY+NKIP  +PLTTI K ++A                    +  + +S T 
Sbjct: 228 ILVFLLGSRFYKNKIPRGSPLTTIFKVLLA-------------------ASIVSCSSKTS 287

Query: 187 SPSTTTPKPTKEPTHTTPPP--TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLP 246
           S   T+ +   E    TP    T +L  LNKAI+    H  L CT QQ+EDVKIVLK+LP
Sbjct: 288 SNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLP 347

Query: 247 IFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLII 306
           IF CTI+LN CLAQLST+SV QA+TMN KI +  +P ASLP+FPV+F+++LAP Y+HLII
Sbjct: 348 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 407

Query: 307 PFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAI---ADAHPAAHPLPITF 366
           PFAR +T++E GITHLQRIGVGL+LSIVAMA+AAL+E+KRK +   A    +   LPITF
Sbjct: 408 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 467

Query: 367 LWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVN 426
           LWI  QYLFLGSADLFTLAGLLEFFFTEAPS+MRSLAT+LSWASLA+GYYLSSV+V +VN
Sbjct: 468 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 527

Query: 427 HVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRST 456
            VT  +  SPWL G+ +N   L  FYWLMC LS +NFLH+LFWA +YKY ST
Sbjct: 528 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 559

BLAST of Cp4.1LG11g07650 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 495.7 bits (1275), Expect = 3.9e-140
Identity = 278/472 (58.90%), Postives = 343/472 (72.67%), Query Frame = 0

Query: 7   GLVILTVEAKAAT------------------AASAAVVFVGLYLVAVGVGGIKGSLPAHG 66
           GL++LT++A+  +                   + AA +FVGLYLV++G+GGIKGSLP+HG
Sbjct: 112 GLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKAAFLFVGLYLVSLGIGGIKGSLPSHG 171

Query: 67  AEQLEEGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVS 126
           AEQ +EGT +GRK RS+FFNY+VF LSCGALVAVT VVW+EDN GWEWGFG+STI+I +S
Sbjct: 172 AEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTFVVWIEDNKGWEWGFGVSTISIFLS 231

Query: 127 IPLFLAGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTR 186
           I +FL GS FY+NKIP  +PLTTI K ++A                    +  + +S T 
Sbjct: 232 ILVFLLGSRFYKNKIPRGSPLTTIFKVLLA-------------------ASIVSCSSKTS 291

Query: 187 SPSTTTPKPTKEPTHTTPPP--TQTLKFLNKAIQTPPFHPSLSCTTQQLEDVKIVLKLLP 246
           S   T+ +   E    TP    T +L  LNKAI+    H  L CT QQ+EDVKIVLK+LP
Sbjct: 292 SNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKIVLKMLP 351

Query: 247 IFACTIILNSCLAQLSTFSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLII 306
           IF CTI+LN CLAQLST+SV QA+TMN KI +  +P ASLP+FPV+F+++LAP Y+HLII
Sbjct: 352 IFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPTYDHLII 411

Query: 307 PFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAI---ADAHPAAHPLPITF 366
           PFAR +T++E GITHLQRIGVGL+LSIVAMA+AAL+E+KRK +   A    +   LPITF
Sbjct: 412 PFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEETLPITF 471

Query: 367 LWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVN 426
           LWI  QYLFLGSADLFTLAGLLEFFFTEAPS+MRSLAT+LSWASLA+GYYLSSV+V +VN
Sbjct: 472 LWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWASLALGYYLSSVMVPIVN 531

Query: 427 HVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRST 456
            VT  +  SPWL G+ +N   L  FYWLMC LS +NFLH+LFWA +YKY ST
Sbjct: 532 RVTKSAGQSPWL-GEKLNRNRLDLFYWLMCVLSVVNFLHYLFWAKRYKYIST 563

BLAST of Cp4.1LG11g07650 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 484.6 bits (1246), Expect = 9.0e-137
Identity = 264/459 (57.52%), Postives = 342/459 (74.51%), Query Frame = 0

Query: 7   GLVILTVEAKAATAA--SAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLEEGTAEGRKGRS 66
           GL++LTV+A   +    S   +FVGLYLVA+GVGGIKGSLP HGAEQ +E T+ GR+ RS
Sbjct: 141 GLMVLTVQAHEHSTEPWSRVFLFVGLYLVALGVGGIKGSLPPHGAEQFDEETSSGRRQRS 200

Query: 67  SFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFLAGSCFYRNKIP 126
            FFNYF+FSLSCGAL+AVT+VVW+EDN GW +GFG+ST AIL+S+P+FLAGS  YR K+P
Sbjct: 201 FFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFGVSTAAILISVPVFLAGSRVYRLKVP 260

Query: 127 AAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASMTRSPSTTTPKPTKEPTHT 186
           + +P+TT+ K +         +AA     ++ +T+   V   TR+    +   TK+  + 
Sbjct: 261 SGSPITTLFKVL---------TAALYAKYKKRRTSRIVVTCHTRNDCDDS--VTKQ--NC 320

Query: 187 TPPPTQTLKFLNKAIQTPPFHP-SLSCTTQQLEDVKIVLKLLPIFACTIILNSCLAQLST 246
                    FL + ++     P  L CT +Q++DVKIV+K+LPIF  TI+LN CLAQLST
Sbjct: 321 DGDDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMSTIMLNCCLAQLST 380

Query: 247 FSVEQASTMNTKIGSIKLPPASLPIFPVLFIILLAPLYNHLIIPFARTLTRTESGITHLQ 306
           FSV+QASTMNTK+GS  +PPA+LP+FPV+F+++LAP YNHL++P AR  T+TE+GITHLQ
Sbjct: 381 FSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARKSTKTETGITHLQ 440

Query: 307 RIGVGLLLSIVAMAIAALIEVKRKAIA--------DAHPAAHPLPITFLWIGFQYLFLGS 366
           RIG GL+LSIVAMA+AAL+E KRK +          +  ++ PLPITFLW+  QY+FLGS
Sbjct: 441 RIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITFLWVAIQYVFLGS 500

Query: 367 ADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVSLVNHVTAKSSHSPWL 426
           ADLFTLAG++EFFFTEAPSTMRSLAT+LSWASLA+GYY SSV+VS VN VT  + H+PWL
Sbjct: 501 ADLFTLAGMMEFFFTEAPSTMRSLATSLSWASLAMGYYFSSVLVSAVNFVTGLNHHNPWL 560

Query: 427 SGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRS 455
            G+++N Y L +FYWLMC LS +NFLH+LFWA +Y YRS
Sbjct: 561 LGENLNQYHLERFYWLMCVLSGINFLHYLFWASRYVYRS 586

BLAST of Cp4.1LG11g07650 vs. TAIR 10
Match: AT5G01180.1 (peptide transporter 5 )

HSP 1 Score: 260.8 bits (665), Expect = 2.1e-69
Identity = 168/475 (35.37%), Postives = 273/475 (57.47%), Query Frame = 0

Query: 7   GLVILTVEAKA-------------ATAASAAVVFVGLYLVAVGVGGIKGSLPAHGAEQLE 66
           G+ +LT+ A               ATA   A+ F+ LYL+A+G GGIK  + + GA+Q +
Sbjct: 110 GMTLLTISASVPGLTPTCSGETCHATAGQTAITFIALYLIALGTGGIKPCVSSFGADQFD 169

Query: 67  EGTAEGRKGRSSFFNYFVFSLSCGALVAVTLVVWMEDNLGWEWGFGISTIAILVSIPLFL 126
           +   + ++ +SSFFN+F F ++ GA++A +++VW++ N+GW WG G+ T+A+ +++  F 
Sbjct: 170 DTDEKEKESKSSFFNWFYFVINVGAMIASSVLVWIQMNVGWGWGLGVPTVAMAIAVVFFF 229

Query: 127 AGSCFYRNKIPAAAPLTTILKSIIAFNDHLDDSAAPLNGRRRNKTASNAVASM--TRSPS 186
           AGS FYR + P  +PLT +L+ I+A               R++K       S+      +
Sbjct: 230 AGSNFYRLQKPGGSPLTRMLQVIVA-------------SCRKSKVKIPEDESLLYENQDA 289

Query: 187 TTTPKPTKEPTHTTPPPTQTLKFLNK-AIQTPPFHPSLS-------CTTQQLEDVKIVLK 246
            ++   +++  H     T+ L F +K A++T   +   +       CT  Q+E++K +++
Sbjct: 290 ESSIIGSRKLEH-----TKILTFFDKAAVETESDNKGAAKSSSWKLCTVTQVEELKALIR 349

Query: 247 LLPIFACTIILNSCLAQLSTFSVEQASTMNTKIG-SIKLPPASLPIFPVLFIILLAPLYN 306
           LLPI+A  I+  S  +Q+ T  V Q +T++  +G + K+P ASL +F  L ++  AP+Y+
Sbjct: 350 LLPIWATGIVFASVYSQMGTVFVLQGNTLDQHMGPNFKIPSASLSLFDTLSVLFWAPVYD 409

Query: 307 HLIIPFARTLTRTESGITHLQRIGVGLLLSIVAMAIAALIEVKRKAIADAHPA--AHPLP 366
            LI+PFAR  T  E G T LQRIG+GL++SI +M  A ++EV R      H       +P
Sbjct: 410 KLIVPFARKYTGHERGFTQLQRIGIGLVISIFSMVSAGILEVARLNYVQTHNLYNEETIP 469

Query: 367 ITFLWIGFQYLFLGSADLFTLAGLLEFFFTEAPSTMRSLATALSWASLAVGYYLSSVIVS 426
           +T  W   QY  +G A++FT  G LEFF+ +AP  MRSL +ALS  ++A G YLS+ +V+
Sbjct: 470 MTIFWQVPQYFLVGCAEVFTFIGQLEFFYDQAPDAMRSLCSALSLTAIAFGNYLSTFLVT 529

Query: 427 LVNHVTAKSSHSPWLSGQDINHYDLHKFYWLMCALSALNFLHFLFWAIKYKYRST 456
           LV  VT       W++ +++N+  L  F+WL+  LS LNFL +L+ A  Y Y+ T
Sbjct: 530 LVTKVTRSGGRPGWIA-KNLNNGHLDYFFWLLAGLSFLNFLVYLWIAKWYTYKKT 565

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8H1571.2e-15462.40Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1[more]
Q8VYE45.5e-13958.90Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1[more]
Q9FM201.3e-13557.52Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2[more]
Q9LFB83.0e-6835.37Protein NRT1/ PTR FAMILY 8.2 OS=Arabidopsis thaliana OX=3702 GN=NPF8.2 PE=2 SV=1[more]
Q9M3905.1e-6835.94Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023546035.11.15e-29997.35protein NRT1/ PTR FAMILY 4.6-like [Cucurbita pepo subsp. pepo][more]
XP_022946066.12.33e-29294.92protein NRT1/ PTR FAMILY 4.6-like [Cucurbita moschata][more]
XP_022999670.17.42e-28291.61protein NRT1/ PTR FAMILY 4.6-like [Cucurbita maxima][more]
XP_038886228.18.19e-23676.17protein NRT1/ PTR FAMILY 4.6-like [Benincasa hispida][more]
XP_008456458.14.75e-22873.89PREDICTED: protein NRT1/ PTR FAMILY 4.6-like [Cucumis melo] >KAA0036440.1 protei... [more]
Match NameE-valueIdentityDescription
A0A6J1G2M61.13e-29294.92protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita moschata OX=3662 GN=LOC111450266 ... [more]
A0A6J1KG663.59e-28291.61protein NRT1/ PTR FAMILY 4.6-like OS=Cucurbita maxima OX=3661 GN=LOC111493955 PE... [more]
A0A5D3BLL02.30e-22873.89Protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C3A12.30e-22873.89protein NRT1/ PTR FAMILY 4.6-like OS=Cucumis melo OX=3656 GN=LOC103496401 PE=3 S... [more]
A0A0A0KHD22.58e-22877.20Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G404120 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G69850.18.6e-15662.40nitrate transporter 1:2 [more]
AT1G27040.23.9e-14058.90Major facilitator superfamily protein [more]
AT1G27040.13.9e-14058.90Major facilitator superfamily protein [more]
AT5G62730.19.0e-13757.52Major facilitator superfamily protein [more]
AT5G01180.12.1e-6935.37peptide transporter 5 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 6..457
e-value: 4.7E-122
score: 409.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 18..453
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 7..414
e-value: 7.5E-93
score: 311.5
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 18..456
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..190
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..190
NoneNo IPR availablePANTHERPTHR11654:SF490PROTEIN NRT1/ PTR FAMILY 4.6-LIKEcoord: 18..456

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g07650.1Cp4.1LG11g07650.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity