Cp4.1LG11g07420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG11g07420
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG11: 5790846 .. 5791917 (-)
RNA-Seq Expression	Cp4.1LG11g07420
Synteny	Cp4.1LG11g07420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTTGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACGGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCCATTCATGTGAAACTTGGAACATACAAGGAGTTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATTATCGTTGACGATCGAAGTAATGGCACTGGATCGACCACAGCAACTTCTGCAACTCTTAGTAAGCTTCAAATTCTCCGCTAACCGATTAAATTTGTGTTAATACTAAAACATATTTCATTTTATAGCTGTAAGAGGTGCAAATTTCATGGCTCAGTCGTTGACATTTCAAAACTCCGCTGGGCCGAAGGAGCACCAAGCTGTTGCAGTTCTTGACCAAGCCAAACACACAGCATACTATAAGTGTGTGTTTTTGGGTTATCAAGATACGCTCTATGCTGGGGGACTACCCCAATTCTTCAGAGAGTGCGATATATACGGAAGCATTGACTTCATATTCGGAAACGGAGTCACCGTATTCCAAGATTGTAATATATATGTTCGAGAATTTGGTAGTAAGACTACGGTAACAGCACAATCGAAAGCAAATATAGATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTTATCGAGTCATTTCTTGATAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAATGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

mRNA sequence

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTTGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACGGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCCATTCATGTGAAACTTGGAACATACAAGGAGTTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATTATCGTTGACGATCGAAATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTTATCGAGTCATTTCTTGATAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAATGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

Coding sequence (CDS)

ATGCCATCATTCAAGCTTGCTATGCTCTTTATAGTCTATTTGCTTGTTACTAACTTTTCTTATTGCTATAGTTTTGATGTCACGGTGTCTTTAGATGGCACGGGAAATTATGTCAAAATTAGTGATGCTATAGCAGCCGCACCAAATTTCAGCACGAAAAGATTTTCCATTCATGTGAAACTTGGAACATACAAGGAGTTGATTGAAGTTCCACCTTCTAAGACATTCATAGCTTTGATTGGGGATGATGCTTCTACCACCATTATCGTTGACGATCGAAATGATCAAAGCGGCTTCGTCTTCCAAAACTGTAAGGTGACCGTGTCGCCCGAGATAGCATCAAGTAAGGACAAGGTATTGTTGTATCTTGGACGGCCATGGAAAGAATATTCAACGGTTGTGTTTATCGAGTCATTTCTTGATAGTGTGGTTCAGCCTAAAGGATGGTTAGGGTGGCCAGATGTACCGGTTGACCAATTGTTTTTTGCAGAGTACAATAATACGGGTCCAGGAGCTGACACTTCTAGAAGAGTGGATTGGCCTGGTTACCATATATTACATACGGCTACACAAGTTACAAGGTTTACCGTTGAAAACTTTATTGGTGGGAGTCAATGGTTGCCTGAAACTGGCATACCATTTAGAACTGGTTTTTAG

Protein sequence

MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDASTTIIVDDRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLGWPDVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPETGIPFRTGF

Homology

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 9.2e-36
Identity = 92/314 (29.30%), Postives = 132/314 (42.04%), Query Frame = 0


Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDD 83
           +++++V++DGTGN+  I+DA+ AAPN S  RF I++K G Y E +E+P  KT I  IGD 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 84  ASTTIIVDDR---------------------------------------------NDQSG 143
              T+I  +R                                             +D S 
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 144 F---------------------------------------VFQ----------------- 203
           F                                       VFQ                 
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 204 -----------------NCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQ 219
                            NC++  +P++   K+    YLGRPW++YS  V I+SF+D ++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 1.2e-35
Identity = 60/127 (47.24%), Postives = 89/127 (70.08%), Query Frame = 0


Query: 92  DRNDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQPKGWLG 151
           D N  +G +  NC+VT + ++   +  V  +LGRPWK+YS  V+I++FLDS++ P GW+ 
Sbjct: 383 DPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWME 442

Query: 152 WP-DVPVDQLFFAEYNNTGPGADTSRRVDWPGYHILHTATQVTRFTVENFIGGSQWLPET 211
           W  D  ++ L++AEY NTGPG+ T+ RV W GYH+L + +QV++FTV NFI G+ WLP T
Sbjct: 443 WSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 502

Query: 212 GIPFRTG 218
            +PF +G
Sbjct: 503 NVPFTSG 509

HSP 2 Score: 49.7 bits (117), Expect = 4.9e-05
Identity = 30/84 (35.71%), Postives = 44/84 (52.38%), Query Frame = 0


Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDAS 85
           ++ V+ DG+GN   I +A+AAA      R+ I++K GTY E IEV      I  +GD   
Sbjct: 202 NIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYIKAGTYNENIEVKLKN--IMFVGDGIG 261

Query: 86  TTIIVDDRNDQSG-FVFQNCKVTV 109
            TII   ++   G   F++  V V
Sbjct: 262 KTIITGSKSVGGGATTFKSATVAV 283

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy Swiss-Prot
Match: Q9LXK7 (Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3702 GN=PME32 PE=2 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 7.8e-35
Identity = 87/311 (27.97%), Postives = 124/311 (39.87%), Query Frame = 0


Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDAS 85
           D  V+ DGTGN+  ISDA+ AAP++STKR+ IHVK G Y E +E+   K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 86  TTIIVDDRNDQSGF---------------------------------------------- 145
            T+I  +R+   G+                                              
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFY 333

Query: 146 --------------------------------------VFQNCKVTVSPEIASSKDKV-- 205
                                                 VFQ+C++     + + K+ +  
Sbjct: 334 RCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITA 393

Query: 206 --------------------------------LLYLGRPWKEYSTVVFIESFLDSVVQPK 218
                                             YLGRPWK YS  VF+++++   + P 
Sbjct: 394 QGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPV 453

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy Swiss-Prot
Match: Q9SMY7 (Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3702 GN=PME44 PE=2 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 2.3e-34
Identity = 89/313 (28.43%), Postives = 126/313 (40.26%), Query Frame = 0


Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDD 83
           ++DV+V+LDGTGN+ KI DAI  AP++S+ RF I++K G Y E +E+   K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 84  ASTTIIVDDRNDQSGF-------------------------------------------- 143
              T+I  +R+   G+                                            
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 144 ----------------------------------------VFQNCKVTVSPEIASSKDKV 203
                                                   VFQNC++     + + K+ +
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTI 389

Query: 204 L----------------------------------LYLGRPWKEYSTVVFIESFLDSVVQ 218
                                               YLGRPWK YS  VFI + +  VV+
Sbjct: 390 TAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVR 449

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy Swiss-Prot
Match: Q9M9W7 (Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3702 GN=PME22 PE=3 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 6.6e-34
Identity = 98/302 (32.45%), Postives = 131/302 (43.38%), Query Frame = 0


Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDAS 85
           +  V++DG G Y  I++AI  APN STKR+ I+VK G YKE I++   KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 86  TTIIVDDRN---------------------------------------------DQSGF- 145
            TII  DRN                                             DQS F 
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFY 359

Query: 146 --------------------------------------VFQNC-----------KVTVSP 205
                                                 V QNC           KVT++ 
Sbjct: 360 RCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITA 419

Query: 206 EIASSKDK----------VL----LYLGRPWKEYSTVVFIESFLDSVVQPKGWLGW-PDV 218
           +   S ++          VL     YLGRPWK YS  V++ +++  +VQP+GWL W  + 
Sbjct: 420 QGRKSPNQNTGFVIQNSYVLATQPTYLGRPWKLYSRTVYMNTYMSQLVQPRGWLEWFGNF 479

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: XP_023546999.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399 bits (1024), Expect = 1.26e-137
Identity = 217/334 (64.97%), Postives = 218/334 (65.27%), Query Frame = 0


Query: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK 60
           MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK
Sbjct: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK 60

Query: 61  LGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN-------------------------- 120
           LGTYKELIEVPPSKTFIALIGDDASTTIIVDDR+                          
Sbjct: 61  LGTYKELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FQNSAGPKEHQAVAVLDQAKHTAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGN 180

Query: 181 ------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLLYLG 218
                                         DQSGFVFQNCKVTVSPEIASSKDKVLLYLG
Sbjct: 181 GVTVFQDCNIYVREFGSKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLG 240

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: XP_022946598.1 (pectinesterase-like [Cucurbita moschata] >KAG6599039.1 Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 392 bits (1008), Expect = 3.43e-135
Identity = 214/334 (64.07%), Postives = 216/334 (64.67%), Query Frame = 0


Query: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK 60
           MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPN STKRFSIHVK
Sbjct: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVK 60

Query: 61  LGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN-------------------------- 120
            GTYKELIEVPPSKTFIALIGDDASTTIIVDDR+                          
Sbjct: 61  PGTYKELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FQNSAGPKEHQAVAVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGN 180

Query: 181 ------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLLYLG 218
                                         DQSGFVFQNCKVTVSPEIASSKDKVLLYLG
Sbjct: 181 GVTVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLG 240

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: XP_022999348.1 (pectinesterase 2-like [Cucurbita maxima])

HSP 1 Score: 382 bits (982), Expect = 3.09e-131
Identity = 208/334 (62.28%), Postives = 215/334 (64.37%), Query Frame = 0


Query: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK 60
           MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSI VK
Sbjct: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVK 60

Query: 61  LGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN-------------------------- 120
            GTY E+IEVPPSKTFIALIG+DASTTIIVDDR+                          
Sbjct: 61  PGTYNEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FQNSAGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGN 180

Query: 181 ------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLLYLG 218
                                         DQSGFVFQNCKVTV+PEIASSKDKVLLYLG
Sbjct: 181 GVSVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLG 240

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 286 bits (731), Expect = 4.65e-93
Identity = 158/336 (47.02%), Postives = 185/336 (55.06%), Query Frame = 0


Query: 3   SFKLAMLFIVYLLVT-----NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSI 62
           +FKL   F+V LLV       FSYC+ F+ TVS DG+GN+V++SDAIAAAPNFST RF I
Sbjct: 2   AFKLLGFFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYI 61

Query: 63  HVKLGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN----------------------- 122
           HVK G YKE +EVP  KTFIALIG+DASTTIIVD+R+                       
Sbjct: 62  HVKPGIYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQ 121

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 122 FLTFENSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFI 181

Query: 183 ---------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLL 217
                                            DQSGF+FQNC VTVSPEIASSKD V +
Sbjct: 182 FGNGLVVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVTM 241

[Close]

BLAST of Cp4.1LG11g07420 vs. NCBI nr
Match: XP_038889244.1 (probable pectinesterase/pectinesterase inhibitor 39 [Benincasa hispida])

HSP 1 Score: 275 bits (702), Expect = 1.73e-88
Identity = 158/350 (45.14%), Postives = 183/350 (52.29%), Query Frame = 0


Query: 1   MPSFKLAMLFIVYLLVT--NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIH 60
           M   K AM+FIVYLLV    FS C+SF+ TVSLDGTGN+VK++DAIAAAPNFST RF IH
Sbjct: 1   MALLKHAMVFIVYLLVVVVEFSDCFSFNCTVSLDGTGNFVKVNDAIAAAPNFSTTRFYIH 60

Query: 61  VKLGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN------------------------ 120
           VK G YKE++EVP  KTFIALIGDDASTTIIV++R+                        
Sbjct: 61  VKRGIYKEIVEVPSQKTFIALIGDDASTTIIVNNRSNATGFTTATTATLSKLQFFSIITL 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 ACLLAVNGENFMAQFLTFENSAGPKGGQAIAVVDQAKHTAYYKCVFLGYQDTLFAASDLQ 180

Query: 181 -----------------------------------------------DQSGFVFQNCKVT 217
                                                          D SGFVFQNC VT
Sbjct: 181 FFKECDIYGSIDFIFGNGLTIFQDCNIFARLPSIESTVTAQSKNRKDDPSGFVFQNCNVT 240

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy TrEMBL
Match: A0A6J1G4B3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450617 PE=4 SV=1)

HSP 1 Score: 392 bits (1008), Expect = 1.66e-135
Identity = 214/334 (64.07%), Postives = 216/334 (64.67%), Query Frame = 0


Query: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK 60
           MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPN STKRFSIHVK
Sbjct: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNLSTKRFSIHVK 60

Query: 61  LGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN-------------------------- 120
            GTYKELIEVPPSKTFIALIGDDASTTIIVDDR+                          
Sbjct: 61  PGTYKELIEVPPSKTFIALIGDDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FQNSAGPKEHQAVAVLDQAKHSAYYKCVFLGYQDTLYAGGLPQFFRECDIYGSIDFIFGN 180

Query: 181 ------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLLYLG 218
                                         DQSGFVFQNCKVTVSPEIASSKDKVLLYLG
Sbjct: 181 GVTVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVSPEIASSKDKVLLYLG 240

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy TrEMBL
Match: A0A6J1KJG6 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111493749 PE=4 SV=1)

HSP 1 Score: 382 bits (982), Expect = 1.50e-131
Identity = 208/334 (62.28%), Postives = 215/334 (64.37%), Query Frame = 0


Query: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVK 60
           MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSI VK
Sbjct: 1   MPSFKLAMLFIVYLLVTNFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIQVK 60

Query: 61  LGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN-------------------------- 120
            GTY E+IEVPPSKTFIALIG+DASTTIIVDDR+                          
Sbjct: 61  PGTYNEVIEVPPSKTFIALIGEDASTTIIVDDRSNGTGSTTATSATLTVRGANFMAQSLT 120

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 121 FQNSAGPKEHQAVAVLDQARHTSYYKCVFLGYQDTLYAGGLPQFFKECDIYGSIDFIFGN 180

Query: 181 ------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLLYLG 218
                                         DQSGFVFQNCKVTV+PEIASSKDKVLLYLG
Sbjct: 181 GVSVFQDCNIYVREFGIKTTVTAQSKANIDDQSGFVFQNCKVTVAPEIASSKDKVLLYLG 240

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy TrEMBL
Match: A0A6J1DAG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1)

HSP 1 Score: 286 bits (731), Expect = 2.25e-93
Identity = 158/336 (47.02%), Postives = 185/336 (55.06%), Query Frame = 0


Query: 3   SFKLAMLFIVYLLVT-----NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSI 62
           +FKL   F+V LLV       FSYC+ F+ TVS DG+GN+V++SDAIAAAPNFST RF I
Sbjct: 2   AFKLLGFFVVSLLVVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYI 61

Query: 63  HVKLGTYKELIEVPPSKTFIALIGDDASTTIIVDDRN----------------------- 122
           HVK G YKE +EVP  KTFIALIG+DASTTIIVD+R+                       
Sbjct: 62  HVKPGIYKEHVEVPVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQ 121

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 122 FLTFENSAEQKEGQAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFI 181

Query: 183 ---------------------------------DQSGFVFQNCKVTVSPEIASSKDKVLL 217
                                            DQSGF+FQNC VTVSPEIASSKD V +
Sbjct: 182 FGNGLVVFQDCNIYARIPGTHIIVTAQSKNAVEDQSGFIFQNCNVTVSPEIASSKDNVTM 241

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy TrEMBL
Match: A0A6J1DCN7 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111019286 PE=4 SV=1)

HSP 1 Score: 269 bits (687), Expect = 1.01e-86
Identity = 150/335 (44.78%), Postives = 178/335 (53.13%), Query Frame = 0


Query: 3   SFKLAMLFIVYLLVT-----NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSI 62
           +F   +LF+V LLV       FSY + F+  VSLDGTGN+V++SDA AAAPNF T RF I
Sbjct: 2   AFNFLVLFVVSLLVVINHFVKFSYSFRFNAVVSLDGTGNFVRVSDATAAAPNFGTTRFYI 61

Query: 63  HVKLGTYKELIEVPPSKTFIALIGDDASTTIIVDDR------------------------ 122
           H+K G YKE +EVP  KTFIALIGDDASTTIIVD+R                        
Sbjct: 62  HIKPGIYKEHVEVPVQKTFIALIGDDASTTIIVDNRSNGSGFSTSATATLTVKGQNFMAQ 121

Query: 123 ------------------------------------------------------------ 182
                                                                       
Sbjct: 122 SLTFQNSAGPNEHQAIALLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGSVDFI 181

Query: 183 -------------------------------NDQSGFVFQNCKVTVSPEIASSKDKVLLY 217
                                          ND SGF+FQ C VTVSP IASS+DKV+++
Sbjct: 182 FGNGLAVFQDCNIYAQLSGIITVTAQSKDKVNDHSGFIFQRCNVTVSPNIASSRDKVIMF 241

[Close]

BLAST of Cp4.1LG11g07420 vs. ExPASy TrEMBL
Match: A0A0A0L1M3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1)

HSP 1 Score: 268 bits (685), Expect = 1.63e-86
Identity = 152/330 (46.06%), Postives = 182/330 (55.15%), Query Frame = 0


Query: 8   MLFIVYLLVT---NFSYCYSFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTY 67
           M+F++YLLV     FS C+SF+ TVSLDGT N++KI+DAIAAAPNFST RF IHVK GTY
Sbjct: 1   MIFVLYLLVVVIVEFSDCFSFNSTVSLDGTENFIKINDAIAAAPNFSTTRFYIHVKPGTY 60

Query: 68  KELIEVPPSKTFIALIGDDASTTIIVDDR------------------------------- 127
           +E+IEVP  KT IALIGDD+STTIIV++R                               
Sbjct: 61  QEIIEVPYEKTCIALIGDDSSTTIIVNNRSNGTGSSTASSATLTVNGSNFMAQFLTFQNT 120

Query: 128 ------------------------------------------------------------ 187
                                                                       
Sbjct: 121 AGPNEGQAIALLDQATHTAYYKCVFLGYQDTLYAGALPQFFKECDIYGSVDFIFGNGLVV 180

Query: 188 -------------------------NDQSGFVFQNCKVTVSPEIASSKDKVLLYLGRPWK 218
                                    N  SGF+FQNCKVTVSPEIASSKDKV ++LGRPW+
Sbjct: 181 FQDCNIYARLFDVQITVTAQSKPSLNSLSGFIFQNCKVTVSPEIASSKDKVTVFLGRPWR 240

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 151.8 bits (382), Expect = 6.6e-37
Identity = 92/314 (29.30%), Postives = 132/314 (42.04%), Query Frame = 0


Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDD 83
           +++++V++DGTGN+  I+DA+ AAPN S  RF I++K G Y E +E+P  KT I  IGD 
Sbjct: 219 NYNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDG 278

Query: 84  ASTTIIVDDR---------------------------------------------NDQSG 143
              T+I  +R                                             +D S 
Sbjct: 279 IGKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAKDISFVNSAGPAKAQAVAFRSGSDHSA 338

Query: 144 F---------------------------------------VFQ----------------- 203
           F                                       VFQ                 
Sbjct: 339 FYRCEFDGYQDTLYVHSAKQFYRECDIYGTIDFIFGNAAVVFQNSSLYARKPNPGHKIAF 398

Query: 204 -----------------NCKVTVSPEIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQ 219
                            NC++  +P++   K+    YLGRPW++YS  V I+SF+D ++ 
Sbjct: 399 TAQSRNQSDQPTGISILNCRILAAPDLIPVKENFKAYLGRPWRKYSRTVIIKSFIDDLIH 458

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 148.7 bits (374), Expect = 5.6e-36
Identity = 87/311 (27.97%), Postives = 124/311 (39.87%), Query Frame = 0


Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDAS 85
           D  V+ DGTGN+  ISDA+ AAP++STKR+ IHVK G Y E +E+   K  I ++GD   
Sbjct: 214 DAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGID 273

Query: 86  TTIIVDDRNDQSGF---------------------------------------------- 145
            T+I  +R+   G+                                              
Sbjct: 274 ATVITGNRSFIDGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFY 333

Query: 146 --------------------------------------VFQNCKVTVSPEIASSKDKV-- 205
                                                 VFQ+C++     + + K+ +  
Sbjct: 334 RCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITA 393

Query: 206 --------------------------------LLYLGRPWKEYSTVVFIESFLDSVVQPK 218
                                             YLGRPWK YS  VF+++++   + P 
Sbjct: 394 QGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPV 453

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR 10
Match: AT4G33220.1 (pectin methylesterase 44 )

HSP 1 Score: 147.1 bits (370), Expect = 1.6e-35
Identity = 89/313 (28.43%), Postives = 126/313 (40.26%), Query Frame = 0


Query: 24  SFDVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDD 83
           ++DV+V+LDGTGN+ KI DAI  AP++S+ RF I++K G Y E +E+   K  I ++GD 
Sbjct: 210 TYDVSVALDGTGNFTKIMDAIKKAPDYSSTRFVIYIKKGLYLENVEIKKKKWNIVMLGDG 269

Query: 84  ASTTIIVDDRNDQSGF-------------------------------------------- 143
              T+I  +R+   G+                                            
Sbjct: 270 IDVTVISGNRSFIDGWTTFRSATFAVSGRGFLARDITFQNTAGPEKHQAVALRSDSDLSV 329

Query: 144 ----------------------------------------VFQNCKVTVSPEIASSKDKV 203
                                                   VFQNC++     + + K+ +
Sbjct: 330 FFRCAMRGYQDTLYTHTMRQFYRECTITGTVDFIFGDGTVVFQNCQILAKRGLPNQKNTI 389

Query: 204 L----------------------------------LYLGRPWKEYSTVVFIESFLDSVVQ 218
                                               YLGRPWK YS  VFI + +  VV+
Sbjct: 390 TAQGRKDVNQPSGFSIQFSNISADADLVPYLNTTRTYLGRPWKLYSRTVFIRNNMSDVVR 449

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR 10
Match: AT3G05620.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 145.6 bits (366), Expect = 4.7e-35
Identity = 98/302 (32.45%), Postives = 131/302 (43.38%), Query Frame = 0


Query: 26  DVTVSLDGTGNYVKISDAIAAAPNFSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDDAS 85
           +  V++DG G Y  I++AI  APN STKR+ I+VK G YKE I++   KT I L+GD   
Sbjct: 240 NTVVAIDGKGKYRTINEAINEAPNHSTKRYVIYVKKGVYKENIDLKKKKTNIMLVGDGIG 299

Query: 86  TTIIVDDRN---------------------------------------------DQSGF- 145
            TII  DRN                                             DQS F 
Sbjct: 300 QTIITGDRNFMQGLTTFRTATVAVSGRGFIAKDITFRNTAGPQNRQAVALRVDSDQSAFY 359

Query: 146 --------------------------------------VFQNC-----------KVTVSP 205
                                                 V QNC           KVT++ 
Sbjct: 360 RCSVEGYQDTLYAHSLRQFYRDCEIYGTIDFIFGNGAAVLQNCKIYTRVPLPLQKVTITA 419

Query: 206 EIASSKDK----------VL----LYLGRPWKEYSTVVFIESFLDSVVQPKGWLGW-PDV 218
           +   S ++          VL     YLGRPWK YS  V++ +++  +VQP+GWL W  + 
Sbjct: 420 QGRKSPNQNTGFVIQNSYVLATQPTYLGRPWKLYSRTVYMNTYMSQLVQPRGWLEWFGNF 479

[Close]

BLAST of Cp4.1LG11g07420 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 144.1 bits (362), Expect = 1.4e-34
Identity = 92/313 (29.39%), Postives = 132/313 (42.17%), Query Frame = 0


Query: 27  VTVSLDGTGNYVKISDAIAAAPN---FSTKRFSIHVKLGTYKELIEVPPSKTFIALIGDD 86
           VTV  +GTGN+  I+ AIAAAPN    S   F I+V  G Y+E +EVP +K ++ +IGD 
Sbjct: 246 VTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDG 305

Query: 87  ASTTIIVDDRNDQSGF-------------------------------------------- 146
            + T+I  +R+   G+                                            
Sbjct: 306 INQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSV 365

Query: 147 ----------------------------------------VFQNCKV------------- 206
                                                   V QNC +             
Sbjct: 366 FYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEV 425

Query: 207 -------------------TVSP--EIASSKDKVLLYLGRPWKEYSTVVFIESFLDSVVQ 218
                              T+ P  ++A+S   V  YLGRPWKEYS  V +++++D  ++
Sbjct: 426 TAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEYSRTVVMQTYIDGFLE 485

The following BLAST results are available for this feature:

BLAST of Cp4.1LG11g07420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	9.2e-36	29.30	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
O04887	1.2e-35	47.24	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
Q9LXK7	7.8e-35	27.97	Probable pectinesterase/pectinesterase inhibitor 32 OS=Arabidopsis thaliana OX=3...	[more]
Q9SMY7	2.3e-34	28.43	Probable pectinesterase/pectinesterase inhibitor 44 OS=Arabidopsis thaliana OX=3...	[more]
Q9M9W7	6.6e-34	32.45	Putative pectinesterase/pectinesterase inhibitor 22 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Cp4.1LG11g07420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023546999.1	1.26e-137	64.97	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022946598.1	3.43e-135	64.07	pectinesterase-like [Cucurbita moschata] >KAG6599039.1 Pectinesterase 2, partial...	[more]
XP_022999348.1	3.09e-131	62.28	pectinesterase 2-like [Cucurbita maxima]	[more]
XP_022150332.1	4.65e-93	47.02	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_038889244.1	1.73e-88	45.14	probable pectinesterase/pectinesterase inhibitor 39 [Benincasa hispida]	[more]

BLAST of Cp4.1LG11g07420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4B3	1.66e-135	64.07	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111450617 PE=4 SV=1	[more]
A0A6J1KJG6	1.50e-131	62.28	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111493749 PE=4 SV=1	[more]
A0A6J1DAG5	2.25e-93	47.02	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018528 PE=4 SV=1	[more]
A0A6J1DCN7	1.01e-86	44.78	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111019286 PE=4 SV=1	[more]
A0A0A0L1M3	1.63e-86	46.06	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G260430 PE=4 SV=1	[more]

BLAST of Cp4.1LG11g07420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G02300.1	6.6e-37	29.30	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G43270.1	5.6e-36	27.97	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G33220.1	1.6e-35	28.43	pectin methylesterase 44	[more]
AT3G05620.1	4.7e-35	32.45	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	1.4e-34	29.39	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 95..218 e-value: 2.7E-40 score: 140.6
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 22..94 e-value: 6.7E-18 score: 67.1
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 91..203 e-value: 7.2E-39 score: 133.3
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 23..98
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 92..217
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 23..98
None	No IPR available	PANTHER	PTHR31707:SF205	PECTINESTERASE/PECTINESTERASE INHIBITOR 22-RELATED	coord: 92..217
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 51..70
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 21..216

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG11g07420.1	Cp4.1LG11g07420.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity