Cp4.1LG11g04630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG11g04630 vs. ExPASy Swiss-Prot
Match: P36591 (Dihydrofolate reductase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dfr1 PE=2 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 8.4e-18
Identity = 63/204 (30.88%), Postives = 97/204 (47.55%), Query Frame = 0

Query: 258 RFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSD------------IE 317
           + LCLHG+  SG +  K++G     + +  +L F   P  A+ ++D            + 
Sbjct: 6   KVLCLHGWIQSGPVFSKKMGSVQKYLSKYAELHFPTGPVVADEEADPNDEEEKKRLAALG 65

Query: 318 GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 377
           G  +   F WF+       Y ++DE L  I  YM + GPFDG +GFSQGA + A L  + 
Sbjct: 66  GEQNGGKFGWFEVEDFKNTYGSWDESLECINQYMQEKGPFDGLIGFSQGAGIGAMLAQML 125

Query: 378 AKG---IALTKVPKIKFVIIVSGAKFRSQSVA-EKAYSSAIGCPSLHFLGKEDFLMP--H 437
             G       + P  KFV+ V G  FR++    +  Y+  +  PSLH  G  D L+P   
Sbjct: 126 QPGQPPNPYVQHPPFKFVVFVGG--FRAEKPEFDHFYNPKLTTPSLHIAGTSDTLVPLAR 185

Query: 438 GQKLLESYIEPVVINHPKGHTIPR 444
            ++L+E      V+ HP  H +P+
Sbjct: 186 SKQLVERCENAHVLLHPGQHIVPQ 207

BLAST of Cp4.1LG11g04630 vs. ExPASy Swiss-Prot
Match: Q94AC1 (Rhodanese-like domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=STR6 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 3.9e-15
Identity = 67/219 (30.59%), Postives = 97/219 (44.29%), Query Frame = 0

Query: 254 RRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF----------PAEGKSD 313
           RRK R LCLHGFR + +  K + G     +    +L F+DAP           P  G  +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 314 ------IEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIEDYMLKHGPFDGFLG 373
                 +   FD P    +     QF P    T+   FD+ L++++    + GPFDG LG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 470

Query: 374 FSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFL 433
           FSQGA ++AA+ G Q +   L      +F ++ SG  F    + E     +I CPSLH  
Sbjct: 471 FSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIF 530

Query: 434 ----GKEDFLMPHGQKLLESYIE---PVVINHPKGHTIP 443
               GK+  ++      L    E     ++ H  GH IP
Sbjct: 531 GSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564

BLAST of Cp4.1LG11g04630 vs. ExPASy Swiss-Prot
Match: Q3SZ07 (Esterase OVCA2 OS=Bos taurus OX=9913 GN=OVCA2 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.5e-14
Identity = 63/204 (30.88%), Postives = 87/204 (42.65%), Query Frame = 0

Query: 258 RFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGI---------F 317
           R LCL GFR S    +++ G    ++  + +L  L  P P    +  EG           
Sbjct: 8   RILCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVVDAAGSEGARPDSGPCPPE 67

Query: 318 DPPYFEWFQFS--------PEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAA 377
           + P   WF            E T  R  +E L  +   + K GPFDG LGFSQGA L+A 
Sbjct: 68  EQPQGWWFSEQEADVFLALEEPTACRGLEEALETVAQALNKLGPFDGILGFSQGAALAAL 127

Query: 378 LPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFLMP-- 437
           +  L   G    + P  +FVI+VSG   R   + E      +  PSLH  G  D ++P  
Sbjct: 128 VCALGQGGD--PRFPLPRFVILVSGFCPRGLGLMEPIMQGPLSLPSLHVFGDTDGVIPSQ 187

Query: 438 HGQKLLESYIEPVVINHPKGHTIP 443
              +L   +   V + H  GH IP
Sbjct: 188 ESMQLCSRFDGAVTLTHSGGHFIP 209

BLAST of Cp4.1LG11g04630 vs. ExPASy Swiss-Prot
Match: Q9D7E3 (Esterase OVCA2 OS=Mus musculus OX=10090 GN=Ovca2 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 4.3e-14
Identity = 67/208 (32.21%), Postives = 92/208 (44.23%), Query Frame = 0

Query: 254 RRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPA------EGKSDIEGI 313
           R+  R LCL GFR S    +++ G    ++  + +L  L  P P       EG     G 
Sbjct: 4   RQTLRVLCLAGFRQSERGFREKTGALRKTLRGRAELVCLSGPHPVPEAAAPEGSCPDSGP 63

Query: 314 FDP---PYFEWFQ------FS--PEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 373
             P   P   WF       FS   E T  R   E L  +   +   GPFDG LGFSQGA 
Sbjct: 64  CSPEEQPRGWWFSEEEADVFSALEESTVCRGLQEALETVARALDTLGPFDGLLGFSQGAA 123

Query: 374 LSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFL 433
           L+A +  L   G    + P  +F+I+VSG  F  + + E    S +  PSLH  G  D +
Sbjct: 124 LAAYVCALGQAGD--PRFPLPRFIILVSG--FCPRGLKEPILQSPMSLPSLHVFGDTDRV 183

Query: 434 MP--HGQKLLESYIEPVVINHPKGHTIP 443
           +P     +L   ++  V + H  GH IP
Sbjct: 184 IPSQESMQLASRFLGAVTLTHSGGHFIP 207

BLAST of Cp4.1LG11g04630 vs. ExPASy Swiss-Prot
Match: Q8WZ82 (Esterase OVCA2 OS=Homo sapiens OX=9606 GN=OVCA2 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 8.1e-13
Identity = 61/208 (29.33%), Postives = 89/208 (42.79%), Query Frame = 0

Query: 254 RRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGI------ 313
           +R  R LCL GFR S    +++ G    ++  + +L  L  P P       EG       
Sbjct: 4   QRPLRVLCLAGFRQSERGFREKTGALRKALRGRAELVCLSGPHPVPDPPGPEGARSDFGS 63

Query: 314 ---FDPPYFEWFQ------FS--PEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 373
               + P   WF       FS   E    R  +E L  +   + + GPFDG LGFSQGA 
Sbjct: 64  CPPEEQPRGWWFSEQEADVFSALEEPAVCRGLEESLGMVAQALNRLGPFDGLLGFSQGAA 123

Query: 374 LSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFL 433
           L+A +  L   G    + P  +F+++VSG   R     E      +  PSLH  G  D +
Sbjct: 124 LAALVCALGQAGD--PRFPLPRFILLVSGFCPRGIGFKESILQRPLSLPSLHVFGDTDKV 183

Query: 434 MPHGQ--KLLESYIEPVVINHPKGHTIP 443
           +P  +  +L   +   + + H  GH IP
Sbjct: 184 IPSQESVQLASQFPGAITLTHSGGHFIP 209

BLAST of Cp4.1LG11g04630 vs. NCBI nr
Match: XP_017254165.1 (PREDICTED: uncharacterized protein LOC108224120 isoform X1 [Daucus carota subsp. sativus])

HSP 1 Score: 498 bits (1283), Expect = 3.61e-170
Identity = 256/490 (52.24%), Postives = 320/490 (65.31%), Query Frame = 0

Query: 16  ISSMGSESRI--QRKPRILCLHGFRTSGAILKKHVEKWPTSVLNRFDFHFMDAPFLSRGK 75
           +  MG +  I  QRKP+ LCLHGFRTSG ILK  + KWP  VL + D  F DAP    GK
Sbjct: 35  VDEMGGQVEIIEQRKPKFLCLHGFRTSGHILKTQLHKWPEFVLEKLDLVFPDAPLPCTGK 94

Query: 76  SEVEGLHDPPYFEWFQFHEDTADFANVVECLQFIENHMLEHGPFDGLLGFSQGAALAALL 135
           S VEG+ DPPY+EW++  +D  ++ N   CL++I+  M++HGPFDGLLGFSQGA ++A L
Sbjct: 95  SGVEGIFDPPYYEWYRPSKDCTEYTNFDGCLEYIQECMIKHGPFDGLLGFSQGAFISAAL 154

Query: 136 PGFQAKVNTPAAWPGYCCKSLFQFSRMKLNRLRQGVALTRVPKIKFVIIING-------- 195
           PG QAK                            G+ALT VPKIKFVIII+G        
Sbjct: 155 PGLQAK----------------------------GLALTEVPKIKFVIIISGGKLHNEAF 214

Query: 196 ------------------EEDFFMPSGLQLLESFVEPFVINHLKGHAVPKLDQKSLKIME 255
                             E DF  P G  LLESFV+P VI+H KGH +P+LD+K L++M 
Sbjct: 215 SEKAFSEAITCPSLHFLGETDFLKPHGTALLESFVDPVVIHHPKGHTIPRLDEKGLELML 274

Query: 256 NFIDRISKSPEFHLQRI------------PIMGTDGEI--RRKPRFLCLHGFRTSGAILK 315
            F+++I K  E   Q +              M  + EI  +RKP+FLCLHGFRTSG ILK
Sbjct: 275 GFLEKIQK--ELTNQEVFSLDYKGFTTFKSEMADEAEIVEQRKPKFLCLHGFRTSGHILK 334

Query: 316 KQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLS 375
            Q+ KWP  VL++LDL   DAPFP  GKS +EGIFDPPY+EWFQ + +FT + NFDECL 
Sbjct: 335 TQLHKWPQFVLDKLDLVIPDAPFPCTGKSSVEGIFDPPYYEWFQPNKDFTVFTNFDECLE 394

Query: 376 FIEDYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSV 435
           +I++ M+KHGPFDG LGFSQGA LSAALPG+QAKG+ALTKVPKIKFVII+SG K +++++
Sbjct: 395 YIQECMIKHGPFDGLLGFSQGAFLSAALPGMQAKGLALTKVPKIKFVIIISGGKLKNEAL 454

Query: 436 AEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVME 463
            +KA+S     PSLH LG+ DFL P G  LLES+ +PVVI+HPKGHTIPRLD+K LE+M 
Sbjct: 455 LQKAFSDVYHFPSLHILGEMDFLKPQGTALLESFADPVVIHHPKGHTIPRLDEKSLELML 494

BLAST of Cp4.1LG11g04630 vs. NCBI nr
Match: XP_017254166.1 (PREDICTED: uncharacterized protein LOC108224120 isoform X2 [Daucus carota subsp. sativus] >XP_017254167.1 PREDICTED: uncharacterized protein LOC108224120 isoform X2 [Daucus carota subsp. sativus])

HSP 1 Score: 497 bits (1279), Expect = 4.12e-170
Identity = 256/487 (52.57%), Postives = 319/487 (65.50%), Query Frame = 0

Query: 19  MGSESRI--QRKPRILCLHGFRTSGAILKKHVEKWPTSVLNRFDFHFMDAPFLSRGKSEV 78
           MG +  I  QRKP+ LCLHGFRTSG ILK  + KWP  VL + D  F DAP    GKS V
Sbjct: 1   MGGQVEIIEQRKPKFLCLHGFRTSGHILKTQLHKWPEFVLEKLDLVFPDAPLPCTGKSGV 60

Query: 79  EGLHDPPYFEWFQFHEDTADFANVVECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGF 138
           EG+ DPPY+EW++  +D  ++ N   CL++I+  M++HGPFDGLLGFSQGA ++A LPG 
Sbjct: 61  EGIFDPPYYEWYRPSKDCTEYTNFDGCLEYIQECMIKHGPFDGLLGFSQGAFISAALPGL 120

Query: 139 QAKVNTPAAWPGYCCKSLFQFSRMKLNRLRQGVALTRVPKIKFVIIING----------- 198
           QAK                            G+ALT VPKIKFVIII+G           
Sbjct: 121 QAK----------------------------GLALTEVPKIKFVIIISGGKLHNEAFSEK 180

Query: 199 ---------------EEDFFMPSGLQLLESFVEPFVINHLKGHAVPKLDQKSLKIMENFI 258
                          E DF  P G  LLESFV+P VI+H KGH +P+LD+K L++M  F+
Sbjct: 181 AFSEAITCPSLHFLGETDFLKPHGTALLESFVDPVVIHHPKGHTIPRLDEKGLELMLGFL 240

Query: 259 DRISKSPEFHLQRI------------PIMGTDGEI--RRKPRFLCLHGFRTSGAILKKQV 318
           ++I K  E   Q +              M  + EI  +RKP+FLCLHGFRTSG ILK Q+
Sbjct: 241 EKIQK--ELTNQEVFSLDYKGFTTFKSEMADEAEIVEQRKPKFLCLHGFRTSGHILKTQL 300

Query: 319 GKWPDSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE 378
            KWP  VL++LDL   DAPFP  GKS +EGIFDPPY+EWFQ + +FT + NFDECL +I+
Sbjct: 301 HKWPQFVLDKLDLVIPDAPFPCTGKSSVEGIFDPPYYEWFQPNKDFTVFTNFDECLEYIQ 360

Query: 379 DYMLKHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEK 438
           + M+KHGPFDG LGFSQGA LSAALPG+QAKG+ALTKVPKIKFVII+SG K +++++ +K
Sbjct: 361 ECMIKHGPFDGLLGFSQGAFLSAALPGMQAKGLALTKVPKIKFVIIISGGKLKNEALLQK 420

Query: 439 AYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFI 463
           A+S     PSLH LG+ DFL P G  LLES+ +PVVI+HPKGHTIPRLD+K LE+M  F+
Sbjct: 421 AFSDVYHFPSLHILGEMDFLKPQGTALLESFADPVVIHHPKGHTIPRLDEKSLELMLGFL 457

BLAST of Cp4.1LG11g04630 vs. NCBI nr
Match: XP_023545459.1 (dihydrofolate reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478 bits (1229), Expect = 5.66e-166
Identity = 233/234 (99.57%), Postives = 234/234 (100.00%), Query Frame = 0

Query: 235 KSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDA 294
           +SPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDA
Sbjct: 16  RSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDA 75

Query: 295 PFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQG 354
           PFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQG
Sbjct: 76  PFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQG 135

Query: 355 AILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKED 414
           AILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKED
Sbjct: 136 AILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKED 195

Query: 415 FLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           FLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE
Sbjct: 196 FLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 249

BLAST of Cp4.1LG11g04630 vs. NCBI nr
Match: XP_022997250.1 (dihydrofolate reductase-like [Cucurbita maxima])

HSP 1 Score: 476 bits (1224), Expect = 3.26e-165
Identity = 231/235 (98.30%), Postives = 235/235 (100.00%), Query Frame = 0

Query: 234 SKSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 293
           ++SPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD
Sbjct: 15  TRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 74

Query: 294 APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 353
           APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ
Sbjct: 75  APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 134

Query: 354 GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 413
           GAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE
Sbjct: 135 GAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 194

Query: 414 DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDD+GLEVMESFIEKISKMLDEAEE
Sbjct: 195 DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKISKMLDEAEE 249

BLAST of Cp4.1LG11g04630 vs. NCBI nr
Match: XP_022961839.1 (dihydrofolate reductase-like [Cucurbita moschata])

HSP 1 Score: 474 bits (1219), Expect = 1.87e-164
Identity = 230/235 (97.87%), Postives = 233/235 (99.15%), Query Frame = 0

Query: 234 SKSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 293
           S+SPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD
Sbjct: 15  SRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 74

Query: 294 APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 353
           APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ
Sbjct: 75  APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 134

Query: 354 GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 413
           GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS  IGCPSLHFLGKE
Sbjct: 135 GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKE 194

Query: 414 DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           DFLMPHGQKLLESYIEPV+INHPKGHTIPRLDD+GLEVMESFIEKISKMLDEAEE
Sbjct: 195 DFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKMLDEAEE 249

BLAST of Cp4.1LG11g04630 vs. ExPASy TrEMBL
Match: A0A6J1K928 (dihydrofolate reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111492210 PE=4 SV=1)

HSP 1 Score: 476 bits (1224), Expect = 1.58e-165
Identity = 231/235 (98.30%), Postives = 235/235 (100.00%), Query Frame = 0

Query: 234 SKSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 293
           ++SPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD
Sbjct: 15  TRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 74

Query: 294 APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 353
           APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ
Sbjct: 75  APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 134

Query: 354 GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 413
           GAILSAALPGLQAKG+ALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE
Sbjct: 135 GAILSAALPGLQAKGMALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 194

Query: 414 DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDD+GLEVMESFIEKISKMLDEAEE
Sbjct: 195 DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDRGLEVMESFIEKISKMLDEAEE 249

BLAST of Cp4.1LG11g04630 vs. ExPASy TrEMBL
Match: A0A6J1HF66 (dihydrofolate reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462484 PE=4 SV=1)

HSP 1 Score: 474 bits (1219), Expect = 9.07e-165
Identity = 230/235 (97.87%), Postives = 233/235 (99.15%), Query Frame = 0

Query: 234 SKSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 293
           S+SPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD
Sbjct: 15  SRSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLD 74

Query: 294 APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 353
           APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ
Sbjct: 75  APFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQ 134

Query: 354 GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKE 413
           GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYS  IGCPSLHFLGKE
Sbjct: 135 GAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSPVIGCPSLHFLGKE 194

Query: 414 DFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           DFLMPHGQKLLESYIEPV+INHPKGHTIPRLDD+GLEVMESFIEKISKMLDEAEE
Sbjct: 195 DFLMPHGQKLLESYIEPVIINHPKGHTIPRLDDEGLEVMESFIEKISKMLDEAEE 249

BLAST of Cp4.1LG11g04630 vs. ExPASy TrEMBL
Match: A0A1R3GDL8 (Serine hydrolase FSH OS=Corchorus capsularis OX=210143 GN=CCACVL1_26695 PE=4 SV=1)

HSP 1 Score: 459 bits (1180), Expect = 2.23e-152
Identity = 239/487 (49.08%), Postives = 305/487 (62.63%), Query Frame = 0

Query: 23  SRIQRKPRILCLHGFRTSGAILKKHVEKWPTSVLNRFDFHFMDAPFLSRGKSEVEGLHDP 82
           SR  RKPRILCLHG+RTSG ILKK +  WP +VL  FDF F DAPF +RGKS++  L+DP
Sbjct: 4   SRASRKPRILCLHGYRTSGEILKKMIGIWPETVLENFDFDFPDAPFAARGKSDIGSLYDP 63

Query: 83  PYFEWFQFHEDTADFANVVECLQFIENHMLEHGPFDGLLGFSQGAALAALLPGFQAKVNT 142
           PY+EWFQ  +      N  EC+ +IE++M++HGPFDGLLGFSQG  LA+++P  Q     
Sbjct: 64  PYYEWFQVKKGEC--LNFEECIAYIEDYMIKHGPFDGLLGFSQGGLLASVIPWMQ----- 123

Query: 143 PAAWPGYCCKSLFQFSRMKLNRLRQGVALTRVPKIKFVIIING----------------- 202
                                  R+GVA T+VPKIKF III+G                 
Sbjct: 124 -----------------------REGVAFTKVPKIKFQIIISGFKLRELKSGPPKLLVNS 183

Query: 203 --------------EEDFFMPSGLQLLESFVEPFVINHLKGHAVPKLDQKSLKIMENFID 262
                         E+D    +G  LL+SFV PFVI+H  GH +PKLD+K L+ M  FI+
Sbjct: 184 FSTPIECPSLHLIGEKDSVKETGFILLKSFVNPFVIHHSMGHTIPKLDEKGLETMLKFIE 243

Query: 263 RISKSPEFHLQ-----RIPIMGTD-GEIR-----RKPRFLCLHGFRTSGAILKKQVGKWP 322
            I K  +  LQ     R  +     GE+      RKPR LCLHGFRTSG IL+K +GKWP
Sbjct: 244 IIQKMSQQDLQIASWPRAALRNRKTGEMLGSQAPRKPRILCLHGFRTSGEILRKMIGKWP 303

Query: 323 DSVLEQLDLDFLDAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYML 382
           ++VLE  D  F DAPFPA GKSD+E ++DPPY+EWFQ +    E  NF+EC+++IEDYM+
Sbjct: 304 ETVLENFDFGFPDAPFPARGKSDVESLYDPPYYEWFQVNE--VECLNFEECIAYIEDYMI 363

Query: 383 KHGPFDGFLGFSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRS-----QSVAE 442
           KHGPFDG LGFSQG +L+A +P +Q +G+A TKVPKIKF II+SG K R        +  
Sbjct: 364 KHGPFDGLLGFSQGGMLAAVIPWMQREGVAFTKVPKIKFQIIISGFKLRELKSGPPKLLV 423

Query: 443 KAYSSAIGCPSLHFLGKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESF 462
            ++S+ I CPSLH +G++DFL   G  LL+S++ P VI+H  GHTIP+LD+KGLE M  F
Sbjct: 424 NSFSTPIECPSLHLIGEKDFLKETGYILLKSFVNPFVIHHSMGHTIPKLDEKGLETMVKF 458

BLAST of Cp4.1LG11g04630 vs. ExPASy TrEMBL
Match: A0A1S3BC97 (dihydrofolate reductase-like OS=Cucumis melo OX=3656 GN=LOC103488098 PE=4 SV=1)

HSP 1 Score: 422 bits (1086), Expect = 1.43e-144
Identity = 201/236 (85.17%), Postives = 219/236 (92.80%), Query Frame = 0

Query: 233 ISKSPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFL 292
           +S  P+FH   +PIMG+D +I  KPRFLCLHGFRTS AILKKQVGKWP SVL+QLDL FL
Sbjct: 14  LSTIPQFHFPTVPIMGSDQKIFTKPRFLCLHGFRTSAAILKKQVGKWPPSVLDQLDLHFL 73

Query: 293 DAPFPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFS 352
           DAPFPAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM++HGPFDGFLGFS
Sbjct: 74  DAPFPAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIEHGPFDGFLGFS 133

Query: 353 QGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGK 412
           QGAILSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS+ IGCPSLHFLG+
Sbjct: 134 QGAILSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGE 193

Query: 413 EDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           EDFLMPHG+KLLESYIEP +I HPKGHTIPRLDDK LEVMESFI++ISKML+E EE
Sbjct: 194 EDFLMPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIQRISKMLNETEE 249

BLAST of Cp4.1LG11g04630 vs. ExPASy TrEMBL
Match: A0A0A0LNX4 (FSH1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G369800 PE=4 SV=1)

HSP 1 Score: 414 bits (1065), Expect = 2.19e-141
Identity = 199/232 (85.78%), Postives = 214/232 (92.24%), Query Frame = 0

Query: 237 PEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF 296
           P+F    IPIMG+D +   KPRFLCLHGFRTS AILKKQVGKWP SVL+QLDL FLDAPF
Sbjct: 18  PQFRFPTIPIMGSDQKFFTKPRFLCLHGFRTSAAILKKQVGKWPLSVLDQLDLHFLDAPF 77

Query: 297 PAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAI 356
           PAEGKSD+EGIFDPPYFEWFQFSPEFTEYRNFDECLSFIE+YM+KHGPFDGFLGFSQGAI
Sbjct: 78  PAEGKSDVEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIENYMIKHGPFDGFLGFSQGAI 137

Query: 357 LSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFL 416
           LSAALPG QAKGIALTKVPKIKFVIIVSGAKFRS+SVAEKAYS+ IGCPSLHFLG+EDFL
Sbjct: 138 LSAALPGFQAKGIALTKVPKIKFVIIVSGAKFRSESVAEKAYSTPIGCPSLHFLGEEDFL 197

Query: 417 MPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 468
           MPHG+KLLESYIEP +I HPKGHTIPRLDDK LEVMESFI +ISK+L+E EE
Sbjct: 198 MPHGKKLLESYIEPTIITHPKGHTIPRLDDKALEVMESFIHRISKILNENEE 249

BLAST of Cp4.1LG11g04630 vs. TAIR 10
Match: AT4G24380.1 (INVOLVED IN: 10-formyltetrahydrofolate biosynthetic process, folic acid and derivative biosynthetic process; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 313.9 bits (803), Expect = 2.1e-85
Identity = 145/223 (65.02%), Postives = 183/223 (82.06%), Query Frame = 0

Query: 247 MGTDG-EIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIE 306
           MG++G  I RKPRFLCLHGFRTSG I+K Q+ KWP SV+++LDL FLDAPFP +GKSD+E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 307 GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 366
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 367 AKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLGKEDFLMPHGQKLLE 426
           AKGIA  KVPKIKFVII+ GAK +S  +AE AYSS++   SLHFLG+ DFL P+G +L+E
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLGETDFLKPYGTQLIE 180

Query: 427 SYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDEAEE 469
           SY  PVV++HPKGHT+PRLD+K LE + +FI+ +  ++ E ++
Sbjct: 181 SYKNPVVVHHPKGHTVPRLDEKSLEKVTAFIDTLEHLVMEEDK 223

BLAST of Cp4.1LG11g04630 vs. TAIR 10
Match: AT5G65400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 5.6e-78
Identity = 136/235 (57.87%), Postives = 173/235 (73.62%), Query Frame = 0

Query: 236 SPEFHLQRIPIMGTDGEIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAP 295
           +PE HL+            + PR LCLHGFRTSG IL+  +GKWPD++L  LDLDFLDAP
Sbjct: 20  APEIHLK-----------PKNPRILCLHGFRTSGRILQAGIGKWPDTILRDLDLDFLDAP 79

Query: 296 FPAEGKSDIEGIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGA 355
           FPA GKSD+E  FDPPY+EW+Q +  F EYRNF+ECL++IEDYM+K+GPFDG LGFSQGA
Sbjct: 80  FPATGKSDVERFFDPPYYEWYQANKGFKEYRNFEECLAYIEDYMIKNGPFDGLLGFSQGA 139

Query: 356 ILSAALPGLQAKGIALTKVPKIKFVIIVSGAK-----FRSQSVAEKAYSSAIGCPSLHFL 415
            L+AA+PG+Q +G ALTKVPK+KF++I+SGAK     F     A  A+SS + CPSLHF+
Sbjct: 140 FLTAAIPGMQEQGSALTKVPKVKFLVIISGAKIPGLMFGEPKAAVNAFSSPVRCPSLHFI 199

Query: 416 GKEDFLMPHGQKLLESYIEPVVINHPKGHTIPRLDDKGLEVMESFIEKISKMLDE 466
           G+ DFL   G+ L+ES++EPVVI+H  GH IP+LD K  E M SF + I +ML +
Sbjct: 200 GERDFLKIEGEVLVESFVEPVVIHHSGGHIIPKLDTKAEETMLSFFQSIRQMLSD 243

BLAST of Cp4.1LG11g04630 vs. TAIR 10
Match: AT4G24380.2 (CONTAINS InterPro DOMAIN/s: Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: alpha/beta-Hydrolases superfamily protein (TAIR:AT5G65400.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 250.0 bits (637), Expect = 3.8e-66
Identity = 117/166 (70.48%), Postives = 139/166 (83.73%), Query Frame = 0

Query: 247 MGTDG-EIRRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPFPAEGKSDIE 306
           MG++G  I RKPRFLCLHGFRTSG I+K Q+ KWP SV+++LDL FLDAPFP +GKSD+E
Sbjct: 1   MGSEGRSIARKPRFLCLHGFRTSGEIMKIQLHKWPKSVIDRLDLVFLDAPFPCQGKSDVE 60

Query: 307 GIFDPPYFEWFQFSPEFTEYRNFDECLSFIEDYMLKHGPFDGFLGFSQGAILSAALPGLQ 366
           GIFDPPY+EWFQF+ EFTEY NF++CL ++ED M+K GPFDG +GFSQGAILS  LPGLQ
Sbjct: 61  GIFDPPYYEWFQFNKEFTEYTNFEKCLEYLEDRMIKLGPFDGLIGFSQGAILSGGLPGLQ 120

Query: 367 AKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFLG 412
           AKGIA  KVPKIKFVII+ GAK +S  +AE AYSS++   SLHFLG
Sbjct: 121 AKGIAFQKVPKIKFVIIIGGAKLKSAKLAENAYSSSLETLSLHFLG 166

BLAST of Cp4.1LG11g04630 vs. TAIR 10
Match: AT1G09280.1 (CONTAINS InterPro DOMAIN/s: Rhodanese-like (InterPro:IPR001763), Serine hydrolase (InterPro:IPR005645); BEST Arabidopsis thaliana protein match is: Rhodanese/Cell cycle control phosphatase superfamily protein (TAIR:AT2G40760.1); Has 5925 Blast hits to 5912 proteins in 1592 species: Archae - 0; Bacteria - 2946; Metazoa - 156; Fungi - 408; Plants - 229; Viruses - 0; Other Eukaryotes - 2186 (source: NCBI BLink). )

HSP 1 Score: 84.3 bits (207), Expect = 2.8e-16
Identity = 67/219 (30.59%), Postives = 97/219 (44.29%), Query Frame = 0

Query: 254 RRKPRFLCLHGFRTSGAILKKQVGKWPDSVLEQLDLDFLDAPF----------PAEGKSD 313
           RRK R LCLHGFR + +  K + G     +    +L F+DAP           P  G  +
Sbjct: 351 RRKLRILCLHGFRQNASSFKGRTGSLAKKLKNIAELVFIDAPHELQFIYQTATPPSGVCN 410

Query: 314 ------IEGIFDPPYFEWF-----QFSP--EFTEYRNFDECLSFIEDYMLKHGPFDGFLG 373
                 +   FD P    +     QF P    T+   FD+ L++++    + GPFDG LG
Sbjct: 411 KKFAWLVSSDFDKPSETGWTVAQCQFDPLQYQTQTEGFDKSLTYLKTAFEEKGPFDGILG 470

Query: 374 FSQGAILSAALPGLQAKGIALTKVPKIKFVIIVSGAKFRSQSVAEKAYSSAIGCPSLHFL 433
           FSQGA ++AA+ G Q +   L      +F ++ SG  F    + E     +I CPSLH  
Sbjct: 471 FSQGAAMAAAVCGKQEQ---LVGEIDFRFCVLCSG--FTPWPLLEMKEKRSIKCPSLHIF 530

Query: 434 ----GKEDFLMPHGQKLLESYIE---PVVINHPKGHTIP 443
               GK+  ++      L    E     ++ H  GH IP
Sbjct: 531 GSQPGKDRQIVTQASSDLAGLFEDGCATIVEHDFGHIIP 564