Cp4.1LG11g04050 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g04050
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationCp4.1LG11: 2263302 .. 2264312 (-)
RNA-Seq ExpressionCp4.1LG11g04050
SyntenyCp4.1LG11g04050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAATGGAAAGAGCGTAATTCTCGACGTCTCTGGCTGGCTCAGGCTTTTCGACGACGGCTCCGTCGACCGGACCTGGACTGGACCGCCGGAGGTCAAGTTTGTGGCGGAGTCTGTGCCGCCTCACGACGACTTCATCGATGGAGTCGCCGTTCGCGACTTGGTGATTGATCGGGAGTCCGGTCTCTGTGTTCGGATTTATCTGCCTGAGGTGAAATGCAGCGGCGGCGGAGAGAAATTGCCGGTTGTTGTTCATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTTTATGTATTATCATACTTATACTGACCTCGTGAAATCCGCCGAAGCAATTTGTGTGTCTGTTTACCTTCGCCGAGCGCCAGAGCATCGTCTTCCCGCGGCTGTTGACGACGGCTTCTCCGCTCTCAGGTGGTTGCAATCGGTGGCTCTAGGTCACGAAATCGAGCCTTGGATTACCGAAAATGCGGATTTCAATCGCGTTTTCCTAATCGGAGATAGCTCTGGCGGAAATCTTGTTCACTCTGTCGCTGCCCTAGCCGGAGAAGCCGATCTCGCTCCAGTGAAACTCGCCGGTGGAATTCCGATTCATCCTGGTTTCGTTCGAGCCGAACGAAGCAAGTCGGAGATCGAGAATCCACAATCGCCGTTCTTGACTCTGGATATGGTAGACAAATTTCTGAGCCTCGCACTACCGGAAGGGAGTACGAAGGATCATCCAATTACATGTCCAATGGGAGCCAACGCGCCGCCGCTTGAAACCCTAAATCTGCCGCCATTTCTTCTGTGCATCGCAGAGAAGGATCTGATCATCGACACCGAAATGGAGTATTACAAAGCAATGAAGGCAGCAAATAAAGAAGTAGAGATTCTGATGAGCAAAGGAATGGGACATAGCTTCTATCTCAACAAGATCGCCCTCAAAATGGACCCCGAAACAGCTGCCGCATCGGACCACCTCTTCGCCGGAATCGCTCGCTTCATCAAGCAGCATTAA

mRNA sequence

ATGACGAATGGAAAGAGCGTAATTCTCGACGTCTCTGGCTGGCTCAGGCTTTTCGACGACGGCTCCGTCGACCGGACCTGGACTGGACCGCCGGAGGTCAAGTTTGTGGCGGAGTCTGTGCCGCCTCACGACGACTTCATCGATGGAGTCGCCGTTCGCGACTTGGTGATTGATCGGGAGTCCGGTCTCTGTGTTCGGATTTATCTGCCTGAGGTGAAATGCAGCGGCGGCGGAGAGAAATTGCCGGTTGTTGTTCATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTTTATGTATTATCATACTTATACTGACCTCGTGAAATCCGCCGAAGCAATTTGTGTGTCTGTTTACCTTCGCCGAGCGCCAGAGCATCGTCTTCCCGCGGCTGTTGACGACGGCTTCTCCGCTCTCAGGTGGTTGCAATCGGTGGCTCTAGGTCACGAAATCGAGCCTTGGATTACCGAAAATGCGGATTTCAATCGCGTTTTCCTAATCGGAGATAGCTCTGGCGGAAATCTTGTTCACTCTGTCGCTGCCCTAGCCGGAGAAGCCGATCTCGCTCCAGTGAAACTCGCCGGTGGAATTCCGATTCATCCTGGTTTCGTTCGAGCCGAACGAAGCAAGTCGGAGATCGAGAATCCACAATCGCCGTTCTTGACTCTGGATATGGTAGACAAATTTCTGAGCCTCGCACTACCGGAAGGGAGTACGAAGGATCATCCAATTACATGTCCAATGGGAGCCAACGCGCCGCCGCTTGAAACCCTAAATCTGCCGCCATTTCTTCTGTGCATCGCAGAGAAGGATCTGATCATCGACACCGAAATGGAGTATTACAAAGCAATGAAGGCAGCAAATAAAGAAGTAGAGATTCTGATGAGCAAAGGAATGGGACATAGCTTCTATCTCAACAAGATCGCCCTCAAAATGGACCCCGAAACAGCTGCCGCATCGGACCACCTCTTCGCCGGAATCGCTCGCTTCATCAAGCAGCATTAA

Coding sequence (CDS)

ATGACGAATGGAAAGAGCGTAATTCTCGACGTCTCTGGCTGGCTCAGGCTTTTCGACGACGGCTCCGTCGACCGGACCTGGACTGGACCGCCGGAGGTCAAGTTTGTGGCGGAGTCTGTGCCGCCTCACGACGACTTCATCGATGGAGTCGCCGTTCGCGACTTGGTGATTGATCGGGAGTCCGGTCTCTGTGTTCGGATTTATCTGCCTGAGGTGAAATGCAGCGGCGGCGGAGAGAAATTGCCGGTTGTTGTTCATTTTCACGGCGGTGGATTTTGTATTAGCGAAGCGGATTGGTTTATGTATTATCATACTTATACTGACCTCGTGAAATCCGCCGAAGCAATTTGTGTGTCTGTTTACCTTCGCCGAGCGCCAGAGCATCGTCTTCCCGCGGCTGTTGACGACGGCTTCTCCGCTCTCAGGTGGTTGCAATCGGTGGCTCTAGGTCACGAAATCGAGCCTTGGATTACCGAAAATGCGGATTTCAATCGCGTTTTCCTAATCGGAGATAGCTCTGGCGGAAATCTTGTTCACTCTGTCGCTGCCCTAGCCGGAGAAGCCGATCTCGCTCCAGTGAAACTCGCCGGTGGAATTCCGATTCATCCTGGTTTCGTTCGAGCCGAACGAAGCAAGTCGGAGATCGAGAATCCACAATCGCCGTTCTTGACTCTGGATATGGTAGACAAATTTCTGAGCCTCGCACTACCGGAAGGGAGTACGAAGGATCATCCAATTACATGTCCAATGGGAGCCAACGCGCCGCCGCTTGAAACCCTAAATCTGCCGCCATTTCTTCTGTGCATCGCAGAGAAGGATCTGATCATCGACACCGAAATGGAGTATTACAAAGCAATGAAGGCAGCAAATAAAGAAGTAGAGATTCTGATGAGCAAAGGAATGGGACATAGCTTCTATCTCAACAAGATCGCCCTCAAAATGGACCCCGAAACAGCTGCCGCATCGGACCACCTCTTCGCCGGAATCGCTCGCTTCATCAAGCAGCATTAA

Protein sequence

MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKGMGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Homology
BLAST of Cp4.1LG11g04050 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 191.8 bits (486), Expect = 1.2e-47
Identity = 108/304 (35.53%), Postives = 165/304 (54.28%), Query Frame = 0

Query: 10  DVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYL 69
           D  G L+L  +G+V R+      +  + + +P  ++    V  +D +  + + L +R+Y 
Sbjct: 13  DCMGLLQLLSNGTVLRS----ESIDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYK 72

Query: 70  PEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAPEHR 129
           P    +     LPVVV FHGGGFC     W  +++    L  S  A+ VS   R APEHR
Sbjct: 73  P--ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHR 132

Query: 130 LPAAVDDGFSALRWLQSVALGHEIEPWITE--NADFNRVFLIGDSSGGNLVHSVAAL--A 189
           LPAA +D  + L WL   A+   +  W  +  + DF+RVF++GDSSGGN+ H +A    +
Sbjct: 133 LPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGS 192

Query: 190 GEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHP 249
           G  +L PV++ G + + P F   ER+ SE   P    L+LD++DKF  L+LP G+T+DH 
Sbjct: 193 GSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHH 252

Query: 250 ITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEY-YKAMKAANKEVEILMSKGMGHS 309
           +  P G  +P LE+++L P L+ +   +L+ D   EY YK  K   K V+ +  +   H 
Sbjct: 253 MANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 307

BLAST of Cp4.1LG11g04050 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 8.3e-44
Identity = 104/307 (33.88%), Postives = 169/307 (55.05%), Query Frame = 0

Query: 7   VILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVR 66
           V+ ++ G +++F+DG V+R    PP V  V+ ++ P           D+ +  ++    R
Sbjct: 28  VVEEIEGLIKVFNDGCVER----PPIVPIVSPTIHPSSK----ATAFDIKLSNDT--WTR 87

Query: 67  IYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAP 126
           +Y+P+   +     LP++V+FHGGGFC+  A W  Y+   T L   A  + VSV  R AP
Sbjct: 88  VYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAP 147

Query: 127 EHRLPAAVDDGFSALRWL--QSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAA- 186
           EHRLPAA DDG + + WL  Q ++ G     W+++  + + VFL GDS+G N+ + VA  
Sbjct: 148 EHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVR 207

Query: 187 -LAGEADLAPVKLAGGIPIHPGFVRAERSKSEIE--NPQSPFLTLDMVDKFLSLALPEGS 246
            +A       + L G I IHP F    R+ SE +  + +S  LTL   D +  LALP G+
Sbjct: 208 IMASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGA 267

Query: 247 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 306
           ++DHP   P+ ++A       LP  ++ +AE D++ +  +E  K M++  K VE ++  G
Sbjct: 268 SRDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 319

Query: 307 MGHSFYL 308
           +GH+F++
Sbjct: 328 VGHAFHI 319

BLAST of Cp4.1LG11g04050 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.0e-42
Identity = 106/305 (34.75%), Postives = 169/305 (55.41%), Query Frame = 0

Query: 7   VILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVR 66
           V+ +V G ++++ DG V+R+   P         V P      GV   D+VID+ + +  R
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLLP--------CVDPSLPLELGVTCSDVVIDKLTNVWAR 82

Query: 67  IYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAP 126
           +Y+P         KLP++V+FHGGGFC+  A W  Y+     L   +  + +SV  R AP
Sbjct: 83  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 142

Query: 127 EHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAA-LA 186
           E+ LPAA +DG +A+ WL           W  +  DF R+FL GDS+GGN+   VAA LA
Sbjct: 143 ENPLPAAYEDGVNAILWLNK---ARNDNLW-AKQCDFGRIFLAGDSAGGNIAQQVAARLA 202

Query: 187 GEADLAPVKLAGGIPIHPGFVRAERSKSE--IENPQSPFLTLDMVDKFLSLALPEGSTKD 246
              DLA +K+ G I I P +   ER++SE  + N ++  LTL   D +  ++LP G+ ++
Sbjct: 203 SPEDLA-LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANRE 262

Query: 247 HPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEV-EILMSKGMG 306
           HP   P+      +++  +   L+C+AE DL++D+ ME    M   N++V + ++ KG+G
Sbjct: 263 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNME----MCDGNEDVIKRVLHKGVG 307

Query: 307 HSFYL 308
           H+F++
Sbjct: 323 HAFHI 307

BLAST of Cp4.1LG11g04050 vs. ExPASy Swiss-Prot
Match: I4DST8 (Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA1 PE=1 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.5e-40
Identity = 99/304 (32.57%), Postives = 163/304 (53.62%), Query Frame = 0

Query: 7   VILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVR 66
           ++LD+  +L ++  G ++R         F+  +V P       VA +D+VID  +G+ VR
Sbjct: 83  IVLDLKPFLIIYKSGRIER---------FLGTTVIP---ACPEVATKDVVIDPATGVSVR 142

Query: 67  IYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAP 126
           +YLP V      +KLPV+V+FHGGGF I       Y++  T L   A  + VS+  R AP
Sbjct: 143 LYLPNV-VDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAP 202

Query: 127 EHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAALAG 186
           E+ LPA+ DD  +   W+ S + G  +EPW+ ++ DF+++ L GDS+GGN+ H VA  A 
Sbjct: 203 EYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRAD 262

Query: 187 EADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPI 246
              +  V +     +HP F+ +E   +EI +P +    ++  DK   LA P+    D P+
Sbjct: 263 AGVIEGVAI-----VHPYFLGSEPVGNEINDPAN----IEFHDKLWRLAAPDTEGLDDPL 322

Query: 247 TCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAA--NKEVEILMSKGMGHS 306
             P+   AP L  L     ++ +A  D +++    YY+A+  +    E E++  +G+GH 
Sbjct: 323 INPVAPGAPSLAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWRGEAELVQHEGVGHV 364

Query: 307 FYLN 309
           F+L+
Sbjct: 383 FHLS 364

BLAST of Cp4.1LG11g04050 vs. ExPASy Swiss-Prot
Match: I4DST9 (Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN=TCEA2 PE=1 SV=1)

HSP 1 Score: 167.5 bits (423), Expect = 2.5e-40
Identity = 99/304 (32.57%), Postives = 163/304 (53.62%), Query Frame = 0

Query: 7   VILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVR 66
           ++LD+  +L ++  G ++R         F+  +V P       VA +D+VID  +G+ VR
Sbjct: 80  IVLDLKPFLIIYKSGRIER---------FLGTTVIP---ACPEVATKDVVIDPATGVSVR 139

Query: 67  IYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAP 126
           +YLP V      +KLPV+V+FHGGGF I       Y++  T L   A  + VS+  R AP
Sbjct: 140 LYLPNV-VDLPSKKLPVLVYFHGGGFVIENTGSPNYHNYLTLLAAKAGVLIVSINYRLAP 199

Query: 127 EHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAALAG 186
           E+ LPA+ DD  +   W+ S + G  +EPW+ ++ DF+++ L GDS+GGN+ H VA  A 
Sbjct: 200 EYPLPASYDDCMAGFNWVVSHSAGPALEPWLAQHGDFSQILLSGDSAGGNVTHYVAMRAD 259

Query: 187 EADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPI 246
              +  V +     +HP F+ +E   +EI +P +    ++  DK   LA P+    D P+
Sbjct: 260 AGVIEGVAI-----VHPYFLGSEPVGNEINDPAN----IEFHDKLWRLAAPDTEGLDDPL 319

Query: 247 TCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAA--NKEVEILMSKGMGHS 306
             P+   AP L  L     ++ +A  D +++    YY+A+  +    E E++  +G+GH 
Sbjct: 320 INPVAPGAPILAGLKCKRAVVFVAGNDFLVERGRMYYEALVKSGWGGEAELVQHEGVGHV 361

Query: 307 FYLN 309
           F+L+
Sbjct: 380 FHLS 361

BLAST of Cp4.1LG11g04050 vs. NCBI nr
Match: XP_023547302.1 (probable carboxylesterase 15 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 690 bits (1781), Expect = 7.58e-251
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE
Sbjct: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV
Sbjct: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS
Sbjct: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336

BLAST of Cp4.1LG11g04050 vs. NCBI nr
Match: KAG6598655.1 (putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 681 bits (1758), Expect = 2.34e-247
Identity = 332/336 (98.81%), Postives = 335/336 (99.70%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MTNGKS++LDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE
Sbjct: 1   MTNGKSIVLDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGLCVRIYLPEVKCSGG EKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV
Sbjct: 61  SGLCVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGEADLAPVKLAGGIPIHPGFVR+ERSKSEIENPQSPFLTLDMVDKFLSLALPEGS
Sbjct: 181 VAALAGEADLAPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of Cp4.1LG11g04050 vs. NCBI nr
Match: XP_022961742.1 (probable carboxylesterase 15 [Cucurbita moschata])

HSP 1 Score: 679 bits (1752), Expect = 1.93e-246
Identity = 330/336 (98.21%), Postives = 335/336 (99.70%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MTNGKS++LDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE
Sbjct: 1   MTNGKSIVLDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGLCVRIYLPEVKCSGG EKLPVVVHFHGGGFCISEADWFMYYHTYTD+VKSAEAICVSV
Sbjct: 61  SGLCVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGEADLAPVKLAGGIPIHPGFVR+ERSKSEIENPQSPFLTLDMVDKFLSLALPEGS
Sbjct: 181 VAALAGEADLAPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYY+AMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYRAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of Cp4.1LG11g04050 vs. NCBI nr
Match: XP_022997210.1 (probable carboxylesterase 15 [Cucurbita maxima])

HSP 1 Score: 667 bits (1720), Expect = 1.46e-241
Identity = 327/336 (97.32%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE
Sbjct: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGL VRIYLPEVKCSGG EKLPVVVHFHGGGFCISEADWFMYYHTYTD+VKSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWI ENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWIIENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGEADL+PVKLAGGIPIHPGFVR+ERSKSEIENPQSPFLTLDMVDKFLSLALPEGS
Sbjct: 181 VAALAGEADLSPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           TKDHPITCPMGANA PLE+LNLPPFLLCIAEKDLIIDTEMEYY+AMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAQPLESLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of Cp4.1LG11g04050 vs. NCBI nr
Match: XP_038886113.1 (probable carboxylesterase 15 [Benincasa hispida])

HSP 1 Score: 631 bits (1628), Expect = 1.54e-227
Identity = 305/336 (90.77%), Postives = 322/336 (95.83%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MT  KS+ILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVID+ 
Sbjct: 1   MTKEKSLILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDQN 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGL VRIYLPEVKC G G+KLPV+VHFHGGGFCISEADWFMYYHTYT+LV+SAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKC-GRGKKLPVIVHFHGGGFCISEADWFMYYHTYTNLVRSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAA+DDGFSAL+WLQSVALG+EIEPWI ENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAIDDGFSALKWLQSVALGNEIEPWIVENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGE DL P+KLAGGIPIHPGFVRA+RSKSE+ENPQSPFLTLDMVDKFLSLALP GS
Sbjct: 181 VAALAGETDLTPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLTLDMVDKFLSLALPVGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           +KDHPITCPMG +APPLE LNLPPFLLCIA+KDLIIDTEMEYYKAMK ANKEVE+LMSKG
Sbjct: 241 SKDHPITCPMGIDAPPLERLNLPPFLLCIADKDLIIDTEMEYYKAMKVANKEVELLMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIA+KMDPETAA SDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIAMKMDPETAAESDHLFAGIARFIKQH 335

BLAST of Cp4.1LG11g04050 vs. ExPASy TrEMBL
Match: A0A6J1HAY3 (probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111462421 PE=4 SV=1)

HSP 1 Score: 679 bits (1752), Expect = 9.33e-247
Identity = 330/336 (98.21%), Postives = 335/336 (99.70%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MTNGKS++LDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE
Sbjct: 1   MTNGKSIVLDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGLCVRIYLPEVKCSGG EKLPVVVHFHGGGFCISEADWFMYYHTYTD+VKSAEAICVSV
Sbjct: 61  SGLCVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGEADLAPVKLAGGIPIHPGFVR+ERSKSEIENPQSPFLTLDMVDKFLSLALPEGS
Sbjct: 181 VAALAGEADLAPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYY+AMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYRAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of Cp4.1LG11g04050 vs. ExPASy TrEMBL
Match: A0A6J1K8Y5 (probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111492182 PE=4 SV=1)

HSP 1 Score: 667 bits (1720), Expect = 7.05e-242
Identity = 327/336 (97.32%), Postives = 332/336 (98.81%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE
Sbjct: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGL VRIYLPEVKCSGG EKLPVVVHFHGGGFCISEADWFMYYHTYTD+VKSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCSGG-EKLPVVVHFHGGGFCISEADWFMYYHTYTDVVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWI ENADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWIIENADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGEADL+PVKLAGGIPIHPGFVR+ERSKSEIENPQSPFLTLDMVDKFLSLALPEGS
Sbjct: 181 VAALAGEADLSPVKLAGGIPIHPGFVRSERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           TKDHPITCPMGANA PLE+LNLPPFLLCIAEKDLIIDTEMEYY+AMKAANKEVEILMSKG
Sbjct: 241 TKDHPITCPMGANAQPLESLNLPPFLLCIAEKDLIIDTEMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 335

BLAST of Cp4.1LG11g04050 vs. ExPASy TrEMBL
Match: A0A5A7V9G7 (Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001000 PE=4 SV=1)

HSP 1 Score: 622 bits (1604), Expect = 3.51e-224
Identity = 299/336 (88.99%), Postives = 318/336 (94.64%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MT GKS++LDVSGWLRL+DDGSVDRTWTGPPEVKFVAESVPPHD+FIDGVAVRDLVID+ 
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGL VRIYLPEVKC    +KLPVVVHFHGGGFCISEADWFMYYHTYT+L KSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGAEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPA ++DGFSAL+WLQSVALG+EIEPWI E ADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAQIEDGFSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAA AGE DLAP+KLAGGIPIHPGFVRA+RSKSEIENPQSPFLTLDMVDKFL+LALP GS
Sbjct: 181 VAARAGETDLAPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           +KDHPITCPMG+ APPLE LNLPPFLLCIAEKDL+IDTEMEYY+AMKAANKEVEILMSKG
Sbjct: 241 SKDHPITCPMGSAAPPLENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALK+DPETAA SD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of Cp4.1LG11g04050 vs. ExPASy TrEMBL
Match: A0A1S3BCL7 (probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103488436 PE=4 SV=1)

HSP 1 Score: 621 bits (1602), Expect = 7.08e-224
Identity = 299/336 (88.99%), Postives = 318/336 (94.64%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MT GKS++LDVSGWLRL+DDGSVDRTWTGPPEVKFVAESVPPHD+FIDGVAVRDLVID+ 
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWTGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGL VRIYLPEVKC G  +KLPVVVHFHGGGFCISEADWFMYYHTYT+L KSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGGEVKKLPVVVHFHGGGFCISEADWFMYYHTYTNLAKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPA ++DGFSAL+WLQSVALG+EIEPWI E ADFNRVFLIGDSSGGNLVHS
Sbjct: 121 YLRRAPEHRLPAQIEDGFSALKWLQSVALGNEIEPWIVEKADFNRVFLIGDSSGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAA AGE DLAP+KLAGGIPIHPGFVRA+RSKSEIENPQSPFLTLDMVDKFL+LALP GS
Sbjct: 181 VAARAGETDLAPLKLAGGIPIHPGFVRAKRSKSEIENPQSPFLTLDMVDKFLNLALPVGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           +KDHPITCPMG+ APPLE LNLPPFLLCIAEKDL+IDTEMEYY+AMKAANKEVEILMSK 
Sbjct: 241 SKDHPITCPMGSAAPPLENLNLPPFLLCIAEKDLVIDTEMEYYEAMKAANKEVEILMSKE 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALK+DPETAA SD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of Cp4.1LG11g04050 vs. ExPASy TrEMBL
Match: A0A0A0KDX8 (Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G401340 PE=4 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 4.57e-220
Identity = 292/336 (86.90%), Postives = 315/336 (93.75%), Query Frame = 0

Query: 1   MTNGKSVILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRE 60
           MT GKS++LDVSGWLRL+DDGSVDRTW GPPEVKFVAESVPPHD+FIDGVAVRDLVID+ 
Sbjct: 1   MTKGKSLVLDVSGWLRLYDDGSVDRTWNGPPEVKFVAESVPPHDEFIDGVAVRDLVIDQN 60

Query: 61  SGLCVRIYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSV 120
           SGL VRIYLPEVKC G  +KLPVV+HFHGGGFCISEADWFMYYHTYT+ VKSAEAICVSV
Sbjct: 61  SGLRVRIYLPEVKCGGEVKKLPVVLHFHGGGFCISEADWFMYYHTYTNFVKSAEAICVSV 120

Query: 121 YLRRAPEHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHS 180
           YLRRAPEHRLPAA++DG S L+WLQSVALG EIEPWI ENADFNRVFLIGDS+GGNLVHS
Sbjct: 121 YLRRAPEHRLPAAIEDGLSGLKWLQSVALGDEIEPWIVENADFNRVFLIGDSAGGNLVHS 180

Query: 181 VAALAGEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGS 240
           VAALAGE DLAP+KLAGGIPIHPGFVRA+RSKSE+ENPQSPFL LDMVD FL+LALP GS
Sbjct: 181 VAALAGETDLAPLKLAGGIPIHPGFVRAKRSKSEMENPQSPFLNLDMVDNFLNLALPVGS 240

Query: 241 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 300
           +KD+PITCPMG  APPLE LNLPPFLLC+AEKDL+IDT+MEYY+AMKAANKEVEILMSKG
Sbjct: 241 SKDNPITCPMGRAAPPLEKLNLPPFLLCVAEKDLVIDTQMEYYEAMKAANKEVEILMSKG 300

Query: 301 MGHSFYLNKIALKMDPETAAASDHLFAGIARFIKQH 336
           MGHSFYLNKIALK+DPETAA SD LFAGIARFIKQH
Sbjct: 301 MGHSFYLNKIALKLDPETAAESDRLFAGIARFIKQH 336

BLAST of Cp4.1LG11g04050 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 8.8e-49
Identity = 108/304 (35.53%), Postives = 165/304 (54.28%), Query Frame = 0

Query: 10  DVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYL 69
           D  G L+L  +G+V R+      +  + + +P  ++    V  +D +  + + L +R+Y 
Sbjct: 13  DCMGLLQLLSNGTVLRS----ESIDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYK 72

Query: 70  PEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAPEHR 129
           P    +     LPVVV FHGGGFC     W  +++    L  S  A+ VS   R APEHR
Sbjct: 73  P--ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHR 132

Query: 130 LPAAVDDGFSALRWLQSVALGHEIEPWITE--NADFNRVFLIGDSSGGNLVHSVAAL--A 189
           LPAA +D  + L WL   A+   +  W  +  + DF+RVF++GDSSGGN+ H +A    +
Sbjct: 133 LPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGS 192

Query: 190 GEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHP 249
           G  +L PV++ G + + P F   ER+ SE   P    L+LD++DKF  L+LP G+T+DH 
Sbjct: 193 GSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHH 252

Query: 250 ITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEY-YKAMKAANKEVEILMSKGMGHS 309
           +  P G  +P LE+++L P L+ +   +L+ D   EY YK  K   K V+ +  +   H 
Sbjct: 253 MANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 307

BLAST of Cp4.1LG11g04050 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.8 bits (486), Expect = 8.8e-49
Identity = 108/304 (35.53%), Postives = 165/304 (54.28%), Query Frame = 0

Query: 10  DVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYL 69
           D  G L+L  +G+V R+      +  + + +P  ++    V  +D +  + + L +R+Y 
Sbjct: 13  DCMGLLQLLSNGTVLRS----ESIDLITQQIPFKNN--QTVLFKDSIYHKPNNLHLRLYK 72

Query: 70  PEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAPEHR 129
           P    +     LPVVV FHGGGFC     W  +++    L  S  A+ VS   R APEHR
Sbjct: 73  P--ISASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHR 132

Query: 130 LPAAVDDGFSALRWLQSVALGHEIEPWITE--NADFNRVFLIGDSSGGNLVHSVAAL--A 189
           LPAA +D  + L WL   A+   +  W  +  + DF+RVF++GDSSGGN+ H +A    +
Sbjct: 133 LPAAFEDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFGS 192

Query: 190 GEADLAPVKLAGGIPIHPGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHP 249
           G  +L PV++ G + + P F   ER+ SE   P    L+LD++DKF  L+LP G+T+DH 
Sbjct: 193 GSIELTPVRVRGYVLMGPFFGGEERTNSE-NGPSEALLSLDLLDKFWRLSLPNGATRDHH 252

Query: 250 ITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEY-YKAMKAANKEVEILMSKGMGHS 309
           +  P G  +P LE+++L P L+ +   +L+ D   EY YK  K   K V+ +  +   H 
Sbjct: 253 MANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKEHG 307

BLAST of Cp4.1LG11g04050 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 179.1 bits (453), Expect = 5.9e-45
Identity = 104/307 (33.88%), Postives = 169/307 (55.05%), Query Frame = 0

Query: 7   VILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVR 66
           V+ ++ G +++F+DG V+R    PP V  V+ ++ P           D+ +  ++    R
Sbjct: 28  VVEEIEGLIKVFNDGCVER----PPIVPIVSPTIHPSSK----ATAFDIKLSNDT--WTR 87

Query: 67  IYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAP 126
           +Y+P+   +     LP++V+FHGGGFC+  A W  Y+   T L   A  + VSV  R AP
Sbjct: 88  VYIPDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAP 147

Query: 127 EHRLPAAVDDGFSALRWL--QSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAA- 186
           EHRLPAA DDG + + WL  Q ++ G     W+++  + + VFL GDS+G N+ + VA  
Sbjct: 148 EHRLPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVR 207

Query: 187 -LAGEADLAPVKLAGGIPIHPGFVRAERSKSEIE--NPQSPFLTLDMVDKFLSLALPEGS 246
            +A       + L G I IHP F    R+ SE +  + +S  LTL   D +  LALP G+
Sbjct: 208 IMASGKYANTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPRGA 267

Query: 247 TKDHPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKG 306
           ++DHP   P+ ++A       LP  ++ +AE D++ +  +E  K M++  K VE ++  G
Sbjct: 268 SRDHPWCNPLMSSA----GAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVHGG 319

Query: 307 MGHSFYL 308
           +GH+F++
Sbjct: 328 VGHAFHI 319

BLAST of Cp4.1LG11g04050 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 1.5e-43
Identity = 106/305 (34.75%), Postives = 169/305 (55.41%), Query Frame = 0

Query: 7   VILDVSGWLRLFDDGSVDRTWTGPPEVKFVAESVPPHDDFIDGVAVRDLVIDRESGLCVR 66
           V+ +V G ++++ DG V+R+   P         V P      GV   D+VID+ + +  R
Sbjct: 23  VVDEVEGLIKVYKDGHVERSQLLP--------CVDPSLPLELGVTCSDVVIDKLTNVWAR 82

Query: 67  IYLPEVKCSGGGEKLPVVVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAP 126
           +Y+P         KLP++V+FHGGGFC+  A W  Y+     L   +  + +SV  R AP
Sbjct: 83  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 142

Query: 127 EHRLPAAVDDGFSALRWLQSVALGHEIEPWITENADFNRVFLIGDSSGGNLVHSVAA-LA 186
           E+ LPAA +DG +A+ WL           W  +  DF R+FL GDS+GGN+   VAA LA
Sbjct: 143 ENPLPAAYEDGVNAILWLNK---ARNDNLW-AKQCDFGRIFLAGDSAGGNIAQQVAARLA 202

Query: 187 GEADLAPVKLAGGIPIHPGFVRAERSKSE--IENPQSPFLTLDMVDKFLSLALPEGSTKD 246
              DLA +K+ G I I P +   ER++SE  + N ++  LTL   D +  ++LP G+ ++
Sbjct: 203 SPEDLA-LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLPRGANRE 262

Query: 247 HPITCPMGANAPPLETLNLPPFLLCIAEKDLIIDTEMEYYKAMKAANKEV-EILMSKGMG 306
           HP   P+      +++  +   L+C+AE DL++D+ ME    M   N++V + ++ KG+G
Sbjct: 263 HPYCKPV---KMIIKSSTVTRTLVCVAEMDLLMDSNME----MCDGNEDVIKRVLHKGVG 307

Query: 307 HSFYL 308
           H+F++
Sbjct: 323 HAFHI 307

BLAST of Cp4.1LG11g04050 vs. TAIR 10
Match: AT5G27320.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.0e-41
Identity = 102/285 (35.79%), Postives = 155/285 (54.39%), Query Frame = 0

Query: 34  KFVAESVPPHDDFIDGVAVRDLVIDRESGLCVRIYLP----------EVKCSGGGEKLPV 93
           +F+   VP + + ++GV   D++IDR++ L  R+Y P          +++    GE +PV
Sbjct: 47  EFLDRKVPANANPVNGVFSFDVIIDRQTNLLSRVYRPADAGTSPSITDLQNPVDGEIVPV 106

Query: 94  VVHFHGGGFCISEADWFMYYHTYTDLVKSAEAICVSVYLRRAPEHRLPAAVDDGFSALRW 153
           +V FHGG F  S A+  +Y      LV    A+ VSV  RRAPE+R P A DDG++ L+W
Sbjct: 107 IVFFHGGSFAHSSANSAIYDTLCRRLVGLCGAVVVSVNYRRAPENRYPCAYDDGWAVLKW 166

Query: 154 LQSVALGHEIEPWITENADFN-RVFLIGDSSGGNLVHSVAALAGEADLAPVKLAGGIPIH 213
           + S         W+    D   R+FL GDSSGGN+VH+VA  A E   + + + G I ++
Sbjct: 167 VNS-------SSWLRSKKDSKVRIFLAGDSSGGNIVHNVAVRAVE---SRIDVLGNILLN 226

Query: 214 PGFVRAERSKSEIENPQSPFLTLDMVDKFLSLALPEGSTKDHPITCPMGANAPPLETLNL 273
           P F   ER++SE       F+T+   D +    LPEG  ++HP   P G  +  LE L+ 
Sbjct: 227 PMFGGTERTESEKRLDGKYFVTVRDRDWYWRAFLPEGEDREHPACSPFGPRSKSLEGLSF 286

Query: 274 PPFLLCIAEKDLIIDTEMEYYKAMKAANKEVEILMSKGMGHSFYL 308
           P  L+ +A  DLI D +++Y + +K A +EV++L  +     FYL
Sbjct: 287 PKSLVVVAGLDLIQDWQLKYAEGLKKAGQEVKLLYLEQATIGFYL 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FG131.2e-4735.53Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q9LFR78.3e-4433.88Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9SX252.0e-4234.75Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
I4DST81.5e-4032.57Tuliposide A-converting enzyme 1, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
I4DST92.5e-4032.57Tuliposide A-converting enzyme 2, chloroplastic OS=Tulipa gesneriana OX=13306 GN... [more]
Match NameE-valueIdentityDescription
XP_023547302.17.58e-251100.00probable carboxylesterase 15 [Cucurbita pepo subsp. pepo][more]
KAG6598655.12.34e-24798.81putative carboxylesterase 17, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022961742.11.93e-24698.21probable carboxylesterase 15 [Cucurbita moschata][more]
XP_022997210.11.46e-24197.32probable carboxylesterase 15 [Cucurbita maxima][more]
XP_038886113.11.54e-22790.77probable carboxylesterase 15 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HAY39.33e-24798.21probable carboxylesterase 15 OS=Cucurbita moschata OX=3662 GN=LOC111462421 PE=4 ... [more]
A0A6J1K8Y57.05e-24297.32probable carboxylesterase 15 OS=Cucurbita maxima OX=3661 GN=LOC111492182 PE=4 SV... [more]
A0A5A7V9G73.51e-22488.99Putative carboxylesterase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sca... [more]
A0A1S3BCL77.08e-22488.99probable carboxylesterase 15 OS=Cucumis melo OX=3656 GN=LOC103488436 PE=4 SV=1[more]
A0A0A0KDX84.57e-22086.90Catalytic OS=Cucumis sativus OX=3659 GN=Csa_6G401340 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G06570.18.8e-4935.53alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.28.8e-4935.53alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.15.9e-4533.88carboxyesterase 17 [more]
AT1G68620.11.5e-4334.75alpha/beta-Hydrolases superfamily protein [more]
AT5G27320.13.0e-4135.79alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 84..306
e-value: 1.8E-50
score: 171.7
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 8..335
e-value: 4.9E-75
score: 254.9
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 7..336
NoneNo IPR availablePANTHERPTHR23024:SF135CELL DEATH ASSOCIATED PROTEINcoord: 4..333
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 4..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g04050.1Cp4.1LG11g04050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity