Cp4.1LG11g03750 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG11g03750 vs. ExPASy Swiss-Prot
Match: Q9C7G0 (65-kDa microtubule-associated protein 8 OS=Arabidopsis thaliana OX=3702 GN=MAP65-8 PE=1 SV=2)

HSP 1 Score: 631.3 bits (1627), Expect = 1.2e-179
Identity = 362/634 (57.10%), Postives = 446/634 (70.35%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           M S QTPI  RSS++L+TSCGYLL+ELQ  W  +GED+F+REKVLLD+EQEC+E YRRKV
Sbjct: 1   MGSLQTPIEMRSSSLLDTSCGYLLRELQMIWDEVGEDKFEREKVLLDIEQECVEAYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           D ANVSR+RLHQELAE+EAE TH LL LGERS+PGRPEK  GTL+EQLDSI PALREMRL
Sbjct: 61  DHANVSRSRLHQELAESEAELTHFLLCLGERSVPGRPEKKGGTLREQLDSIAPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RK+ER+KQ  +++ +IQKISAEI G++   DS+R +  +  DLS  KLEEYQNEL RLH 
Sbjct: 121 RKDERVKQFRSVKGEIQKISAEIAGRSTYEDSTRKITIDDNDLSNKKLEEYQNELHRLHD 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQ+V+ YI  +  LSATLG +AS IITKIHPSL+D +G SKNISD IL+KL GTV
Sbjct: 181 EKNERLQKVDIYICAIRDLSATLGTEASMIITKIHPSLNDLYGISKNISDDILKKLNGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
            SL+EEK +RLEK                                               
Sbjct: 241 VSLEEEKHKRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LH+LG+ L+NLW LMD+ Y DR+ F HVI LLS +  
Sbjct: 301 -----------------------LHHLGRALSNLWNLMDASYEDRQKFFHVIDLLSSAPS 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           ++  PGS+TL II+QAEAEVKRLDQLKAS++KELFIKKQ ELED CN SHM  PS + M 
Sbjct: 361 DVCAPGSITLDIIQQAEAEVKRLDQLKASRIKELFIKKQKELEDTCNMSHMETPS-TEMG 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
            I NL+ SGE+DH +LL +MDE+I+RAKEEA+SRK I+EKV++WML  DEERWLEEY+ D
Sbjct: 421 NITNLVDSGEVDHVDLLAAMDEKIARAKEEAASRKGIIEKVDRWMLASDEERWLEEYDQD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           ++RYSVSR AH+NLRRAERARI V+KI  LVE +L K +SWE ER KVFLY+EVPL+ +L
Sbjct: 481 ENRYSVSRNAHRNLRRAERARITVSKISGLVESILVKAKSWEVERQKVFLYNEVPLVAML 540

Query: 541 EEYNALRQEKE-EKRRPREKKKVQ-THTLIEPENFYGPRPSTSSRRLSNASINGGYSNAI 600
           +EYN LRQEKE EK+R RE KK+     + E +NFY  RP++S+RR+SN S+NGG+ +  
Sbjct: 541 QEYNKLRQEKEMEKQRLREMKKMSIPQPVAEGDNFYMARPASSNRRISNRSMNGGFGSGS 560

Query: 601 ALNRRLSQGILQFGSNNINSATQGISFIKEAKKS 632
            +NR+ S G   F + N N    G S  +E++KS
Sbjct: 601 PINRKYSGG---FNNTNNNYTALGTSIRRESRKS 560

BLAST of Cp4.1LG11g03750 vs. ExPASy Swiss-Prot
Match: Q9FLP0 (65-kDa microtubule-associated protein 1 OS=Arabidopsis thaliana OX=3702 GN=MAP65-1 PE=1 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 2.2e-120
Identity = 269/612 (43.95%), Postives = 376/612 (61.44%), Query Frame = 0

Query: 10  TRSSTMLETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRAR 69
           T S  + E +CG LL++LQ  W  +GE   +R+K+LL +EQECL+VY+RKV+ A  SRA 
Sbjct: 6   TESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAAKSRAE 65

Query: 70  LHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQL 129
           L Q L++A AE + L +SLG++SL G P+K +GT+KEQL +I PAL ++  +KEER+++ 
Sbjct: 66  LLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEERVREF 125

Query: 130 SAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRV 189
           S +Q QIQKI  +I G   N     V + +++DLS+ KL+++Q++LQ L  EK++RL++V
Sbjct: 126 SDVQSQIQKICGDIAGGLSN----EVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLRKV 185

Query: 190 EKYIDTVHKLSATLGMDASTIITKIHPSLD-DPFGRSKNISDSILEKLKGTVESLKEEKQ 249
            +++ TVH L A LG+D  + +T++HPSLD D   +SK+IS+  L +L  TV +LK++K+
Sbjct: 186 LEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDDKK 245

Query: 250 QRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNT 309
           QRL+K                                                       
Sbjct: 246 QRLQK------------------------------------------------------- 305

Query: 310 VNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSL 369
                          L  L   L +LW LMD+P  +R  F HV   +S S  E++ PG+L
Sbjct: 306 ---------------LQELATQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGAL 365

Query: 370 TLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHS 429
              +IEQAE EV RLDQLKAS+MKE+  KKQ ELE+I  ++H+ +   S  E+I++LI S
Sbjct: 366 ARDLIEQAEVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDS 425

Query: 430 GEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSR 489
           G ++   LL  MD QIS+AKEEA SRK I+++VEKWM  C+EE WLE+YN DQ+RYS SR
Sbjct: 426 GNVEPTELLADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNRDQNRYSASR 485

Query: 490 GAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQ 549
           GAH NL+RAE+ARI V+KIPA+V+ L+ KTR+WE E S  F YD VPLL +L+EY  LRQ
Sbjct: 486 GAHLNLKRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQ 542

Query: 550 EK-EEKRRPREKKKVQTHTLIEPENFYGPRPS----TSSRRLSNASINGGYSNAIALNRR 609
           E+ EEKRR RE+KKVQ    +E E+ +  RPS     S+++      N G +N    NRR
Sbjct: 546 EREEEKRRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVGPRANNGGANG-THNRR 542

Query: 610 LSQGILQFGSNN 615
           LS    Q GS +
Sbjct: 606 LSLNANQNGSRS 542

BLAST of Cp4.1LG11g03750 vs. ExPASy Swiss-Prot
Match: Q8LEG3 (65-kDa microtubule-associated protein 2 OS=Arabidopsis thaliana OX=3702 GN=MAP65-2 PE=1 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.8e-112
Identity = 266/646 (41.18%), Postives = 377/646 (58.36%), Query Frame = 0

Query: 17  ETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELAE 76
           E +CG LLQ+LQ  W  +GE   +R+K+LL +E+ECL VY++KV+ A  SRA L Q L++
Sbjct: 13  EITCGTLLQKLQEIWDEVGESDEERDKLLLQIEEECLNVYKKKVELAAKSRAELLQTLSD 72

Query: 77  AEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQVQI 136
           A  E ++L  +LGE+S    P+K +GT+KEQL +I PAL ++  +KEER++  S +Q QI
Sbjct: 73  ATVELSNLTTALGEKSYIDIPDKTSGTIKEQLSAIAPALEQLWQQKEERVRAFSDVQSQI 132

Query: 137 QKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYIDTV 196
           QKI  EI G   N       V ++TDLS+ +L+++Q +LQ L  EK++RLQ+V +++ TV
Sbjct: 133 QKICEEIAGGLNNGPH----VVDETDLSLKRLDDFQRKLQELQKEKSDRLQKVLEFVSTV 192

Query: 197 HKLSATLGMDASTIITKIHPSLDDPFG-RSKNISDSILEKLKGTVESLKEEKQQRLEKVM 256
           H L A L +D  + +T++HPSLD+  G ++K+IS+  L +L  TV +LKE+K QRL+K  
Sbjct: 193 HDLCAVLRLDFLSTVTEVHPSLDEANGVQTKSISNETLARLAKTVLTLKEDKMQRLKK-- 252

Query: 257 KLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNTVNKTIFD 316
                                                                       
Sbjct: 253 ------------------------------------------------------------ 312

Query: 317 KFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIEQ 376
                   L  L   LT+LW LMD+   +R  F HV   +S S  E++  G+L L +IEQ
Sbjct: 313 --------LQELATQLTDLWNLMDTSDEERELFDHVTSNISASVHEVTASGALALDLIEQ 372

Query: 377 AEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHSGEIDHAN 436
           AE EV RLDQLK+S+MKE+  KKQ ELE+I  ++H+ I      E+I++LI +G  +   
Sbjct: 373 AEVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPTE 432

Query: 437 LLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNLR 496
           LL  MD QI++AKEEA SRK I+++VEKWM  C+EE WLE+YN DQ+RYS SRGAH NL+
Sbjct: 433 LLADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNLK 492

Query: 497 RAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKE-EKR 556
           RAE+ARI V+KI A+V+ L+ KTR+WE E S  F YD VPLL +L+EY  LRQE+E EKR
Sbjct: 493 RAEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYDGVPLLAMLDEYTMLRQEREDEKR 552

Query: 557 RPREKKKVQTHTLIEPENFYGPRPS----TSSRRLSNASINGGYSNAIALNRRLSQGILQ 616
           R +E+KK Q     + E+ +G +PS     S+++     +NGG  N   + RRLS    Q
Sbjct: 553 RLKEQKKQQEQPHTDQESAFGSKPSPARPVSAKKPVGTRVNGGGLNETPM-RRLSMNSNQ 569

Query: 617 FGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVS 656
            GS              ++K+     IA   N+ +  +D+ AS VS
Sbjct: 613 NGS--------------KSKRDSLNKIASPSNIVANTKDDAASPVS 569

BLAST of Cp4.1LG11g03750 vs. ExPASy Swiss-Prot
Match: Q9FHM4 (65-kDa microtubule-associated protein 3 OS=Arabidopsis thaliana OX=3702 GN=MAP65-3 PE=1 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 3.8e-101
Identity = 237/598 (39.63%), Postives = 346/598 (57.86%), Query Frame = 0

Query: 16  LETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELA 75
           +ET+CG LL ELQ  W  +GE + DR+++LL+LE+ECLEVYRRKVD AN  RA+L Q +A
Sbjct: 12  VETTCGSLLFELQIIWDEVGETETDRDQMLLELERECLEVYRRKVDQANRCRAQLRQAIA 71

Query: 76  EAEAEFTHLLLSLGERSLPGR-PEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQV 135
           +AEA+   +  ++GER +  R  ++  G+LK++L  I P L EM+ RK ER  Q   +  
Sbjct: 72  DAEAQLAAICSAMGERPVHIRQSDQSVGSLKQELGRILPELEEMQKRKVERRNQFIVVME 131

Query: 136 QIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYID 195
           QI  I+ +I+G+ E V S  ++  ++T+LSM KLEE   +LQ L  EK +R++ + K++ 
Sbjct: 132 QIDSITNDIKGQGELVHSEPLI--DETNLSMRKLEELHCQLQVLQKEKIDRVETIRKHLC 191

Query: 196 TVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTVESLKEEKQQRLEKV 255
           T++   + LGMD + ++ +++P+L DP G  +++SD  +EKL   V+ L E K QR+++ 
Sbjct: 192 TLYSHCSVLGMDFNEVVGQVNPTLSDPEG-PRSLSDHTIEKLGAAVQKLMEVKIQRMQR- 251

Query: 256 MKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNTVNKTIF 315
                                                                       
Sbjct: 252 ------------------------------------------------------------ 311

Query: 316 DKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIE 375
                    L +L  T+  LW LMD+P  +++ + H+   ++ S  EI++  SL+   I+
Sbjct: 312 ---------LQDLATTMLELWNLMDTPIEEQQEYQHITCNIAASEHEITEANSLSEDFIK 371

Query: 376 QAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHSGEIDHA 435
             EAEV RLD++KASKMKEL +KK+ ELE+IC K+H++  S S +++ I  I SG +D  
Sbjct: 372 YVEAEVVRLDEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAIDQTIVAIESGIVDAT 431

Query: 436 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNL 495
            +L  +++ IS+ KEEA SRK I+E+VEKW+  CDEE WLEEYN D +RY+  RGAH  L
Sbjct: 432 MVLEHLEQHISKIKEEALSRKEILERVEKWLSACDEESWLEEYNRDDNRYNAGRGAHLTL 491

Query: 496 RRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKEEK- 555
           +RAE+AR  V K+P +VE L  KT  WE E    FLYD + LL +LEEYN LRQE+EE+ 
Sbjct: 492 KRAEKARNLVTKLPGMVEALASKTIVWEQENGIEFLYDGIRLLSMLEEYNILRQEREEEH 530

Query: 556 RRPREKKKVQTHTLIEPENFYGPRPSTS----SRRLSNASINGGYSNAIALNRRLSQG 607
           RR R++KK+Q   + E E  YG +PS S     ++    S  G      A NRRLS G
Sbjct: 552 RRQRDQKKLQGQLIAEQEALYGSKPSPSKPLGGKKAPRMSTGG------ASNRRLSLG 530

BLAST of Cp4.1LG11g03750 vs. ExPASy Swiss-Prot
Match: Q9SIS3 (65-kDa microtubule-associated protein 6 OS=Arabidopsis thaliana OX=3702 GN=MAP65-6 PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.1e-100
Identity = 227/600 (37.83%), Postives = 352/600 (58.67%), Query Frame = 0

Query: 18  TSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELAEA 77
           T+C  LL+ELQ  W+ +GE + +++++L++LE+ECL++Y+RKVD+A  S+A+LHQ +A  
Sbjct: 16  TTCNNLLRELQKIWVEIGETETEKDRMLMELERECLQIYQRKVDEAANSKAKLHQSVASI 75

Query: 78  EAEFTHLLLSLGERSL--PGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQVQ 137
           EAE   L+ +LG  ++  P + +K + +LKE+L ++TP + E+R++KEERMKQ S I+ Q
Sbjct: 76  EAEVASLMAALGVLNINSPIKLDKGSKSLKEKLAAVTPLVEELRIQKEERMKQFSDIKAQ 135

Query: 138 IQKISAEIEGKAENVDSSRVV--VENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYI 197
           I+KIS EI G +++++ +  +     + DL++  L EYQ  L+ L  EK++RL +V  Y+
Sbjct: 136 IEKISGEISGYSDHLNKAMNISLTLEEQDLTLRNLNEYQTHLRTLQKEKSDRLNKVLGYV 195

Query: 198 DTVHKLSATLGMDASTIITKIHPSLD-DPFGRSKNISDSILEKLKGTVESLKEEKQQRLE 257
           + VH L   LG+D S  ++ +HPSL      +S NISDS LE L+  ++ LK E++ R +
Sbjct: 196 NEVHALCGVLGVDFSQTVSAVHPSLHRTDQEQSTNISDSTLEGLEHMIQKLKTERKSRFQ 255

Query: 258 KVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNTVNKT 317
           K+  +V                                                      
Sbjct: 256 KLKDVV------------------------------------------------------ 315

Query: 318 IFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYI 377
                            +L  LW LMD+P  DR  F  V  ++  S   I++PG L+   
Sbjct: 316 ----------------ASLFELWNLMDTPQEDRTKFGKVTYVVRSSEANITEPGILSTET 375

Query: 378 IEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHSGEID 437
           IEQ   EV  L +LKAS+MKEL +K++ ELED+C  +H+   + +  EK   LI SG +D
Sbjct: 376 IEQVSTEVDSLSKLKASRMKELVMKRRSELEDLCRLTHIQPDTSTSAEKSTALIDSGLVD 435

Query: 438 HANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHK 497
            + LL +++ QI++ K+EA SRK IM+++++W+  C+EE WLEEYN+D++RYS  RG H 
Sbjct: 436 PSELLANIEMQINKIKDEAQSRKDIMDRIDRWLSACEEENWLEEYNLDENRYSAGRGGHV 495

Query: 498 NLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALR-QEKE 557
           NL+RAERAR+ +NKIP +V+ L++KT  WE +  K FLYD V L+ +LE+Y   R Q++E
Sbjct: 496 NLKRAERARVTINKIPGMVDTLIKKTLVWEEDMQKSFLYDGVRLVNILEDYKLTRKQQEE 545

Query: 558 EKRRPREKKKVQTHTLIEPENFYG----PRPSTSSRRLSNASINGGYSNAIALNRRLSQG 607
           EK+R R++KK Q   L + E+ YG    PR S+S R+ +  +I+ G  +     RR S G
Sbjct: 556 EKKRYRDQKKRQDLLLTQRESIYGSKPSPRRSSSFRKPNGFNISNGNGSVPPTPRRGSVG 545

BLAST of Cp4.1LG11g03750 vs. NCBI nr
Match: XP_023547241.1 (65-kDa microtubule-associated protein 8 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1073 bits (2775), Expect = 0.0
Identity = 589/664 (88.70%), Postives = 590/664 (88.86%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIGTRSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLLDLEQECLEVYRRKV
Sbjct: 1   MASFQTPIGTRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLDLEQECLEVYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL
Sbjct: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS
Sbjct: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV
Sbjct: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 241 ESLKEEKQQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP
Sbjct: 301 -----------------------LHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME
Sbjct: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD
Sbjct: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL
Sbjct: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540

Query: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 600
           EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL
Sbjct: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 594

Query: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 660
           NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG
Sbjct: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 594

Query: 661 PISP 663
           PISP
Sbjct: 661 PISP 594

BLAST of Cp4.1LG11g03750 vs. NCBI nr
Match: KAG6598612.1 (65-kDa microtubule-associated protein 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1062 bits (2747), Expect = 0.0
Identity = 583/664 (87.80%), Postives = 586/664 (88.25%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIGTRSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLLDLEQECLEVYRRKV
Sbjct: 1   MASFQTPIGTRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLDLEQECLEVYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL
Sbjct: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS
Sbjct: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKY+DTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV
Sbjct: 181 EKNERLQRVEKYMDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEK QRLEK                                               
Sbjct: 241 ESLKEEKHQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP
Sbjct: 301 -----------------------LHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHM+IPSRSGME
Sbjct: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMVIPSRSGME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD
Sbjct: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL
Sbjct: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540

Query: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 600
           EEYNALRQEKEEKRRPREKKKVQTH LIEPENFYGPRPST SRRLSNASINGGYSNAIAL
Sbjct: 541 EEYNALRQEKEEKRRPREKKKVQTHILIEPENFYGPRPSTGSRRLSNASINGGYSNAIAL 594

Query: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 660
           NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETAS VSTFSG
Sbjct: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASEVSTFSG 594

Query: 661 PISP 663
           PISP
Sbjct: 661 PISP 594

BLAST of Cp4.1LG11g03750 vs. NCBI nr
Match: XP_022962475.1 (65-kDa microtubule-associated protein 8-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 582/664 (87.65%), Postives = 586/664 (88.25%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIGTRSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLLDLEQECLEVYRRKV
Sbjct: 1   MASFQTPIGTRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLDLEQECLEVYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDS+TPALREMRL
Sbjct: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSVTPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS
Sbjct: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV
Sbjct: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 241 ESLKEEKQQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGKTLTNLWELMDSPY DRR FSHVIRLLSFSSP
Sbjct: 301 -----------------------LHNLGKTLTNLWELMDSPYSDRRLFSHVIRLLSFSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSL LYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHM+IPSRSGME
Sbjct: 361 EISDPGSLALYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMVIPSRSGME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD
Sbjct: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL
Sbjct: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540

Query: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 600
           EEYNALRQEKEEKRRPREKKKVQTH LIEPE+FYGPRPSTSSRRLSNASINGGYSNAIAL
Sbjct: 541 EEYNALRQEKEEKRRPREKKKVQTHILIEPESFYGPRPSTSSRRLSNASINGGYSNAIAL 594

Query: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 660
           NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG
Sbjct: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 594

Query: 661 PISP 663
           PISP
Sbjct: 661 PISP 594

BLAST of Cp4.1LG11g03750 vs. NCBI nr
Match: XP_022997093.1 (65-kDa microtubule-associated protein 8 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 581/664 (87.50%), Postives = 586/664 (88.25%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIGTRSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLLDLEQECLEVYRRKV
Sbjct: 1   MASFQTPIGTRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLDLEQECLEVYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL
Sbjct: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RK ERMKQLSAIQVQIQKISAEIEGKAENVDSSRVV+ENKTDLS+TKLEEYQNELQRLHS
Sbjct: 121 RKAERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVIENKTDLSVTKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV
Sbjct: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 241 ESLKEEKQQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGKTLTNLWELMDSPYGDRR FSHVIRLLSFSSP
Sbjct: 301 -----------------------LHNLGKTLTNLWELMDSPYGDRRLFSHVIRLLSFSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHM+IPSRSGME
Sbjct: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMVIPSRSGME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD
Sbjct: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL
Sbjct: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540

Query: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 600
           EEYNALRQEKEEKRRPREKKKV TH LIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL
Sbjct: 541 EEYNALRQEKEEKRRPREKKKVPTHILIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 594

Query: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 660
           NRRLSQGILQFGSNNINSATQGISFIKEAKKSPG+NIARRHNLDSRVRDETASVVSTFSG
Sbjct: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGKNIARRHNLDSRVRDETASVVSTFSG 594

Query: 661 PISP 663
           PISP
Sbjct: 661 PISP 594

BLAST of Cp4.1LG11g03750 vs. NCBI nr
Match: KAG7029548.1 (65-kDa microtubule-associated protein 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 951 bits (2459), Expect = 0.0
Identity = 532/634 (83.91%), Postives = 534/634 (84.23%), Query Frame = 0

Query: 30  WIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELAEAEAEFTHLLLSLGE 89
           W  +GEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELAEAEAEFTHLLLSLGE
Sbjct: 3   WDEVGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELAEAEAEFTHLLLSLGE 62

Query: 90  RSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQVQIQKISAEIEGKAENV 149
           RSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQVQIQKISAEIEGKAENV
Sbjct: 63  RSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQVQIQKISAEIEGKAENV 122

Query: 150 DSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYIDTVHKLSATLGMDASTI 209
           DSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKY+DTVHKLSATLGMDASTI
Sbjct: 123 DSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYMDTVHKLSATLGMDASTI 182

Query: 210 ITKIHPSLDDPFGRSKNISDSILEKLKGTVESLKEEKQQRLEKVMKLVLAKQHIYATSQN 269
           ITKIHPSLDDPFGRSKNISDSILEKLKGTVESLKEEK QRLEK                 
Sbjct: 183 ITKIHPSLDDPFGRSKNISDSILEKLKGTVESLKEEKHQRLEK----------------- 242

Query: 270 QKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNTVNKTIFDKFLQCFLQLHNLGKT 329
                                                                LHNLGKT
Sbjct: 243 -----------------------------------------------------LHNLGKT 302

Query: 330 LTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIEQAEAEVKRLDQLKASK 389
           LTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIEQAEAEVKRLDQLKASK
Sbjct: 303 LTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIEQAEAEVKRLDQLKASK 362

Query: 390 MKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHSGEIDHANLLTSMDEQISRAKEE 449
           MKELFIKKQMELEDI                        EIDHANLLTSMDEQISRAKEE
Sbjct: 363 MKELFIKKQMELEDIW-----------------------EIDHANLLTSMDEQISRAKEE 422

Query: 450 ASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNLRRAERARIAVNKIPAL 509
           ASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNLRRAERARIAVNKIPAL
Sbjct: 423 ASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNLRRAERARIAVNKIPAL 482

Query: 510 VELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKEEKRRPREKKKVQTHTLIEP 569
           VELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKEEKRRPREKKKVQTH LIEP
Sbjct: 483 VELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKEEKRRPREKKKVQTHILIEP 542

Query: 570 ENFYGPRPSTSSRRLSNASINGGYSNAIALNRRLSQGILQFGSNNINSATQGISFIKEAK 629
           ENFYGPRPST SRRLSNASINGGYSNAIALNRRLSQGILQFGSNNINSATQGISFIKEAK
Sbjct: 543 ENFYGPRPSTGSRRLSNASINGGYSNAIALNRRLSQGILQFGSNNINSATQGISFIKEAK 543

Query: 630 KSPGQNIARRHNLDSRVRDETASVVSTFSGPISP 663
           KSPGQNIARRHNLDSRVRDETAS VSTFSGPISP
Sbjct: 603 KSPGQNIARRHNLDSRVRDETASEVSTFSGPISP 543

BLAST of Cp4.1LG11g03750 vs. ExPASy TrEMBL
Match: A0A6J1HF78 (65-kDa microtubule-associated protein 8-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462900 PE=3 SV=1)

HSP 1 Score: 1060 bits (2742), Expect = 0.0
Identity = 582/664 (87.65%), Postives = 586/664 (88.25%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIGTRSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLLDLEQECLEVYRRKV
Sbjct: 1   MASFQTPIGTRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLDLEQECLEVYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDS+TPALREMRL
Sbjct: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSVTPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS
Sbjct: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV
Sbjct: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 241 ESLKEEKQQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGKTLTNLWELMDSPY DRR FSHVIRLLSFSSP
Sbjct: 301 -----------------------LHNLGKTLTNLWELMDSPYSDRRLFSHVIRLLSFSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSL LYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHM+IPSRSGME
Sbjct: 361 EISDPGSLALYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMVIPSRSGME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD
Sbjct: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL
Sbjct: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540

Query: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 600
           EEYNALRQEKEEKRRPREKKKVQTH LIEPE+FYGPRPSTSSRRLSNASINGGYSNAIAL
Sbjct: 541 EEYNALRQEKEEKRRPREKKKVQTHILIEPESFYGPRPSTSSRRLSNASINGGYSNAIAL 594

Query: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 660
           NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG
Sbjct: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 594

Query: 661 PISP 663
           PISP
Sbjct: 661 PISP 594

BLAST of Cp4.1LG11g03750 vs. ExPASy TrEMBL
Match: A0A6J1KCW4 (65-kDa microtubule-associated protein 8 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492115 PE=3 SV=1)

HSP 1 Score: 1059 bits (2738), Expect = 0.0
Identity = 581/664 (87.50%), Postives = 586/664 (88.25%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIGTRSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLLDLEQECLEVYRRKV
Sbjct: 1   MASFQTPIGTRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLDLEQECLEVYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL
Sbjct: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RK ERMKQLSAIQVQIQKISAEIEGKAENVDSSRVV+ENKTDLS+TKLEEYQNELQRLHS
Sbjct: 121 RKAERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVIENKTDLSVTKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV
Sbjct: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 241 ESLKEEKQQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGKTLTNLWELMDSPYGDRR FSHVIRLLSFSSP
Sbjct: 301 -----------------------LHNLGKTLTNLWELMDSPYGDRRLFSHVIRLLSFSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHM+IPSRSGME
Sbjct: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMVIPSRSGME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD
Sbjct: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL
Sbjct: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540

Query: 541 EEYNALRQEKEEKRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 600
           EEYNALRQEKEEKRRPREKKKV TH LIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL
Sbjct: 541 EEYNALRQEKEEKRRPREKKKVPTHILIEPENFYGPRPSTSSRRLSNASINGGYSNAIAL 594

Query: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFSG 660
           NRRLSQGILQFGSNNINSATQGISFIKEAKKSPG+NIARRHNLDSRVRDETASVVSTFSG
Sbjct: 601 NRRLSQGILQFGSNNINSATQGISFIKEAKKSPGKNIARRHNLDSRVRDETASVVSTFSG 594

Query: 661 PISP 663
           PISP
Sbjct: 661 PISP 594

BLAST of Cp4.1LG11g03750 vs. ExPASy TrEMBL
Match: A0A6J1BSB0 (65-kDa microtubule-associated protein 8 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005092 PE=3 SV=1)

HSP 1 Score: 935 bits (2417), Expect = 0.0
Identity = 526/665 (79.10%), Postives = 547/665 (82.26%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           MASFQTPIG RSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLL+LEQECLEVYR+KV
Sbjct: 1   MASFQTPIGMRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLNLEQECLEVYRKKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           D ANVSRARLHQELAEAEAEFT LLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL
Sbjct: 61  DGANVSRARLHQELAEAEAEFTQLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEERMKQ  A+Q QI KISAEI G++E  DSS VV+ N+ DLS+ KLEEYQNELQRLHS
Sbjct: 121 RKEERMKQFKAVQGQILKISAEIGGQSECDDSSPVVIVNENDLSLKKLEEYQNELQRLHS 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQRVEKYI+TVHKLSATLGMDASTIITKIHPSLDDP G SKNISDSILEKLK TV
Sbjct: 181 EKNERLQRVEKYIETVHKLSATLGMDASTIITKIHPSLDDPSGISKNISDSILEKLKRTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 241 ESLKEEKQQRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LH LGK LTNLW+LMDSPYGDRR FSHV RLLS SSP
Sbjct: 301 -----------------------LHKLGKALTNLWDLMDSPYGDRRLFSHVTRLLSVSSP 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EISDPGSL +YIIEQAEAEVKRLDQLKASKMKELFIKKQ ELEDICNKSHM IPSRS ME
Sbjct: 361 EISDPGSLAVYIIEQAEAEVKRLDQLKASKMKELFIKKQKELEDICNKSHMEIPSRSEME 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLI+SGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWML CDEERWLEEY+MD
Sbjct: 421 KIINLINSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLACDEERWLEEYSMD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARI VNKIPALVELLL KT SWEAERSKVFLYDEVPLL +L
Sbjct: 481 QSRYSVSRGAHKNLRRAERARITVNKIPALVELLLVKTTSWEAERSKVFLYDEVPLLAML 540

Query: 541 EEYNALRQEKEE-KRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIA 600
           EEYNALRQEKEE KRR REKKKVQT  +IEPENFYGPRPSTSSRRLSNASINGGYSNA+A
Sbjct: 541 EEYNALRQEKEEEKRRLREKKKVQTQVVIEPENFYGPRPSTSSRRLSNASINGGYSNAMA 595

Query: 601 LNRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFS 660
           LNRRLS GI Q GSN++NSATQGISFIKE K SPGQN+  RH L S+VRDETASVVSTFS
Sbjct: 601 LNRRLSLGIQQLGSNSMNSATQGISFIKEGKVSPGQNMTLRHTLVSQVRDETASVVSTFS 595

Query: 661 GPISP 663
           GPISP
Sbjct: 661 GPISP 595

BLAST of Cp4.1LG11g03750 vs. ExPASy TrEMBL
Match: A0A1S3BCX0 (65-kDa microtubule-associated protein 8 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488500 PE=3 SV=1)

HSP 1 Score: 930 bits (2403), Expect = 0.0
Identity = 516/665 (77.59%), Postives = 550/665 (82.71%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           M+SFQTPIG RSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLL+LEQECLEVYR+KV
Sbjct: 2   MSSFQTPIGMRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLELEQECLEVYRKKV 61

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           D ANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKM GTLKEQLDSITPALREMRL
Sbjct: 62  DGANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMTGTLKEQLDSITPALREMRL 121

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEER+KQ  ++Q QI KISAEI G++E  DSS VV+ N+ DLS+ KLEEYQNELQRLHS
Sbjct: 122 RKEERVKQFRSVQGQILKISAEIGGQSECDDSSPVVIVNENDLSLNKLEEYQNELQRLHS 181

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQR  KYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDS+LEKLK TV
Sbjct: 182 EKNERLQRAGKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSVLEKLKCTV 241

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 242 ESLKEEKQQRLEK----------------------------------------------- 301

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGK LTNLW+LMDSPYGDRR FSHVIRLLS SSP
Sbjct: 302 -----------------------LHNLGKALTNLWDLMDSPYGDRRLFSHVIRLLSVSSP 361

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EIS+PGSLT+YIIEQAEAEV+RLDQLKASKMKELFIKKQ +LED+CNKSHM IPSRS ME
Sbjct: 362 EISEPGSLTVYIIEQAEAEVRRLDQLKASKMKELFIKKQKDLEDVCNKSHMEIPSRSEME 421

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLI+SGEIDHANLL SMDEQI RAKEEA SRKTIMEKVEKWML CDEERWLEEY+MD
Sbjct: 422 KIINLINSGEIDHANLLASMDEQILRAKEEAYSRKTIMEKVEKWMLACDEERWLEEYSMD 481

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKT++WEAERSKVFLYDEVPLL +L
Sbjct: 482 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTKNWEAERSKVFLYDEVPLLAML 541

Query: 541 EEYNALRQEKEE-KRRPREKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAIA 600
           EEYNALRQEKEE KRR REKKKVQT  +I+P+NFYGPRPSTSSRRLSNASINGGYSNAIA
Sbjct: 542 EEYNALRQEKEEEKRRLREKKKVQTQVIIQPDNFYGPRPSTSSRRLSNASINGGYSNAIA 596

Query: 601 LNRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTFS 660
           LN+R+S GI Q  SN+INS+TQGIS +KE +KSP QNIA RHNL S+VRDETASVVSTFS
Sbjct: 602 LNKRISLGIQQLESNSINSSTQGISILKEGRKSPVQNIALRHNLVSQVRDETASVVSTFS 596

Query: 661 GPISP 663
           GPISP
Sbjct: 662 GPISP 596

BLAST of Cp4.1LG11g03750 vs. ExPASy TrEMBL
Match: A0A1S3BCW3 (65-kDa microtubule-associated protein 8 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488500 PE=3 SV=1)

HSP 1 Score: 926 bits (2392), Expect = 0.0
Identity = 513/666 (77.03%), Postives = 550/666 (82.58%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQA-WIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           M+SFQTPIG RSSTMLETSCGYLLQELQ  W  +GEDQFDREKVLL+LEQECLEVYR+KV
Sbjct: 2   MSSFQTPIGMRSSTMLETSCGYLLQELQMIWDEVGEDQFDREKVLLELEQECLEVYRKKV 61

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           D ANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKM GTLKEQLDSITPALREMRL
Sbjct: 62  DGANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMTGTLKEQLDSITPALREMRL 121

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RKEER+KQ  ++Q QI KISAEI G++E  DSS VV+ N+ DLS+ KLEEYQNELQRLHS
Sbjct: 122 RKEERVKQFRSVQGQILKISAEIGGQSECDDSSPVVIVNENDLSLNKLEEYQNELQRLHS 181

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQR  KYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDS+LEKLK TV
Sbjct: 182 EKNERLQRAGKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSVLEKLKCTV 241

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
           ESLKEEKQQRLEK                                               
Sbjct: 242 ESLKEEKQQRLEK----------------------------------------------- 301

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LHNLGK LTNLW+LMDSPYGDRR FSHVIRLLS SSP
Sbjct: 302 -----------------------LHNLGKALTNLWDLMDSPYGDRRLFSHVIRLLSVSSP 361

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           EIS+PGSLT+YIIEQAEAEV+RLDQLKASKMKELFIKKQ +LED+CNKSHM IPSRS ME
Sbjct: 362 EISEPGSLTVYIIEQAEAEVRRLDQLKASKMKELFIKKQKDLEDVCNKSHMEIPSRSEME 421

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
           KIINLI+SGEIDHANLL SMDEQI RAKEEA SRKTIMEKVEKWML CDEERWLEEY+MD
Sbjct: 422 KIINLINSGEIDHANLLASMDEQILRAKEEAYSRKTIMEKVEKWMLACDEERWLEEYSMD 481

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKT++WEAERSKVFLYDEVPLL +L
Sbjct: 482 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTKNWEAERSKVFLYDEVPLLAML 541

Query: 541 EEYNALRQEKEEKRRP--REKKKVQTHTLIEPENFYGPRPSTSSRRLSNASINGGYSNAI 600
           EEYNALRQEKEE++R   +EKKKVQT  +I+P+NFYGPRPSTSSRRLSNASINGGYSNAI
Sbjct: 542 EEYNALRQEKEEEKRRLRQEKKKVQTQVIIQPDNFYGPRPSTSSRRLSNASINGGYSNAI 597

Query: 601 ALNRRLSQGILQFGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVSTF 660
           ALN+R+S GI Q  SN+INS+TQGIS +KE +KSP QNIA RHNL S+VRDETASVVSTF
Sbjct: 602 ALNKRISLGIQQLESNSINSSTQGISILKEGRKSPVQNIALRHNLVSQVRDETASVVSTF 597

Query: 661 SGPISP 663
           SGPISP
Sbjct: 662 SGPISP 597

BLAST of Cp4.1LG11g03750 vs. TAIR 10
Match: AT1G27920.1 (microtubule-associated protein 65-8 )

HSP 1 Score: 631.3 bits (1627), Expect = 8.6e-181
Identity = 362/634 (57.10%), Postives = 446/634 (70.35%), Query Frame = 0

Query: 1   MASFQTPIGTRSSTMLETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKV 60
           M S QTPI  RSS++L+TSCGYLL+ELQ  W  +GED+F+REKVLLD+EQEC+E YRRKV
Sbjct: 1   MGSLQTPIEMRSSSLLDTSCGYLLRELQMIWDEVGEDKFEREKVLLDIEQECVEAYRRKV 60

Query: 61  DDANVSRARLHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRL 120
           D ANVSR+RLHQELAE+EAE TH LL LGERS+PGRPEK  GTL+EQLDSI PALREMRL
Sbjct: 61  DHANVSRSRLHQELAESEAELTHFLLCLGERSVPGRPEKKGGTLREQLDSIAPALREMRL 120

Query: 121 RKEERMKQLSAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHS 180
           RK+ER+KQ  +++ +IQKISAEI G++   DS+R +  +  DLS  KLEEYQNEL RLH 
Sbjct: 121 RKDERVKQFRSVKGEIQKISAEIAGRSTYEDSTRKITIDDNDLSNKKLEEYQNELHRLHD 180

Query: 181 EKNERLQRVEKYIDTVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTV 240
           EKNERLQ+V+ YI  +  LSATLG +AS IITKIHPSL+D +G SKNISD IL+KL GTV
Sbjct: 181 EKNERLQKVDIYICAIRDLSATLGTEASMIITKIHPSLNDLYGISKNISDDILKKLNGTV 240

Query: 241 ESLKEEKQQRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKS 300
            SL+EEK +RLEK                                               
Sbjct: 241 VSLEEEKHKRLEK----------------------------------------------- 300

Query: 301 SNANFSNTVNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSP 360
                                  LH+LG+ L+NLW LMD+ Y DR+ F HVI LLS +  
Sbjct: 301 -----------------------LHHLGRALSNLWNLMDASYEDRQKFFHVIDLLSSAPS 360

Query: 361 EISDPGSLTLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGME 420
           ++  PGS+TL II+QAEAEVKRLDQLKAS++KELFIKKQ ELED CN SHM  PS + M 
Sbjct: 361 DVCAPGSITLDIIQQAEAEVKRLDQLKASRIKELFIKKQKELEDTCNMSHMETPS-TEMG 420

Query: 421 KIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMD 480
            I NL+ SGE+DH +LL +MDE+I+RAKEEA+SRK I+EKV++WML  DEERWLEEY+ D
Sbjct: 421 NITNLVDSGEVDHVDLLAAMDEKIARAKEEAASRKGIIEKVDRWMLASDEERWLEEYDQD 480

Query: 481 QSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELL 540
           ++RYSVSR AH+NLRRAERARI V+KI  LVE +L K +SWE ER KVFLY+EVPL+ +L
Sbjct: 481 ENRYSVSRNAHRNLRRAERARITVSKISGLVESILVKAKSWEVERQKVFLYNEVPLVAML 540

Query: 541 EEYNALRQEKE-EKRRPREKKKVQ-THTLIEPENFYGPRPSTSSRRLSNASINGGYSNAI 600
           +EYN LRQEKE EK+R RE KK+     + E +NFY  RP++S+RR+SN S+NGG+ +  
Sbjct: 541 QEYNKLRQEKEMEKQRLREMKKMSIPQPVAEGDNFYMARPASSNRRISNRSMNGGFGSGS 560

Query: 601 ALNRRLSQGILQFGSNNINSATQGISFIKEAKKS 632
            +NR+ S G   F + N N    G S  +E++KS
Sbjct: 601 PINRKYSGG---FNNTNNNYTALGTSIRRESRKS 560

BLAST of Cp4.1LG11g03750 vs. TAIR 10
Match: AT5G55230.1 (microtubule-associated proteins 65-1 )

HSP 1 Score: 434.5 bits (1116), Expect = 1.5e-121
Identity = 269/612 (43.95%), Postives = 376/612 (61.44%), Query Frame = 0

Query: 10  TRSSTMLETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRAR 69
           T S  + E +CG LL++LQ  W  +GE   +R+K+LL +EQECL+VY+RKV+ A  SRA 
Sbjct: 6   TESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAAKSRAE 65

Query: 70  LHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQL 129
           L Q L++A AE + L +SLG++SL G P+K +GT+KEQL +I PAL ++  +KEER+++ 
Sbjct: 66  LLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEERVREF 125

Query: 130 SAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRV 189
           S +Q QIQKI  +I G   N     V + +++DLS+ KL+++Q++LQ L  EK++RL++V
Sbjct: 126 SDVQSQIQKICGDIAGGLSN----EVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLRKV 185

Query: 190 EKYIDTVHKLSATLGMDASTIITKIHPSLD-DPFGRSKNISDSILEKLKGTVESLKEEKQ 249
            +++ TVH L A LG+D  + +T++HPSLD D   +SK+IS+  L +L  TV +LK++K+
Sbjct: 186 LEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDDKK 245

Query: 250 QRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNT 309
           QRL+K                                                       
Sbjct: 246 QRLQK------------------------------------------------------- 305

Query: 310 VNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSL 369
                          L  L   L +LW LMD+P  +R  F HV   +S S  E++ PG+L
Sbjct: 306 ---------------LQELATQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGAL 365

Query: 370 TLYIIEQAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHS 429
              +IEQAE EV RLDQLKAS+MKE+  KKQ ELE+I  ++H+ +   S  E+I++LI S
Sbjct: 366 ARDLIEQAEVEVDRLDQLKASRMKEIAFKKQSELEEIYARAHVEVNPESARERIMSLIDS 425

Query: 430 GEIDHANLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSR 489
           G ++   LL  MD QIS+AKEEA SRK I+++VEKWM  C+EE WLE+YN DQ+RYS SR
Sbjct: 426 GNVEPTELLADMDSQISKAKEEAFSRKDILDRVEKWMSACEEESWLEDYNRDQNRYSASR 485

Query: 490 GAHKNLRRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQ 549
           GAH NL+RAE+ARI V+KIPA+V+ L+ KTR+WE E S  F YD VPLL +L+EY  LRQ
Sbjct: 486 GAHLNLKRAEKARILVSKIPAMVDTLVAKTRAWEEEHSMSFAYDGVPLLAMLDEYGMLRQ 542

Query: 550 EK-EEKRRPREKKKVQTHTLIEPENFYGPRPS----TSSRRLSNASINGGYSNAIALNRR 609
           E+ EEKRR RE+KKVQ    +E E+ +  RPS     S+++      N G +N    NRR
Sbjct: 546 EREEEKRRLREQKKVQEQPHVEQESAFSTRPSPARPVSAKKTVGPRANNGGANG-THNRR 542

Query: 610 LSQGILQFGSNN 615
           LS    Q GS +
Sbjct: 606 LSLNANQNGSRS 542

BLAST of Cp4.1LG11g03750 vs. TAIR 10
Match: AT5G55230.2 (microtubule-associated proteins 65-1 )

HSP 1 Score: 419.1 bits (1076), Expect = 6.7e-117
Identity = 269/641 (41.97%), Postives = 376/641 (58.66%), Query Frame = 0

Query: 10  TRSSTMLETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRAR 69
           T S  + E +CG LL++LQ  W  +GE   +R+K+LL +EQECL+VY+RKV+ A  SRA 
Sbjct: 6   TESPHLGEITCGTLLEKLQEIWDEVGESDDERDKLLLQIEQECLDVYKRKVEQAAKSRAE 65

Query: 70  LHQELAEAEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQL 129
           L Q L++A AE + L +SLG++SL G P+K +GT+KEQL +I PAL ++  +KEER+++ 
Sbjct: 66  LLQTLSDANAELSSLTMSLGDKSLVGIPDKSSGTIKEQLAAIAPALEQLWQQKEERVREF 125

Query: 130 SAIQVQIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRV 189
           S +Q QIQKI  +I G   N     V + +++DLS+ KL+++Q++LQ L  EK++RL++V
Sbjct: 126 SDVQSQIQKICGDIAGGLSN----EVPIVDESDLSLKKLDDFQSQLQELQKEKSDRLRKV 185

Query: 190 EKYIDTVHKLSATLGMDASTIITKIHPSLD-DPFGRSKNISDSILEKLKGTVESLKEEKQ 249
            +++ TVH L A LG+D  + +T++HPSLD D   +SK+IS+  L +L  TV +LK++K+
Sbjct: 186 LEFVSTVHDLCAVLGLDFLSTVTEVHPSLDEDTSVQSKSISNETLSRLAKTVLTLKDDKK 245

Query: 250 QRLEKVMKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNT 309
           QRL+K                                                       
Sbjct: 246 QRLQK------------------------------------------------------- 305

Query: 310 VNKTIFDKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSL 369
                          L  L   L +LW LMD+P  +R  F HV   +S S  E++ PG+L
Sbjct: 306 ---------------LQELATQLIDLWNLMDTPDEERELFDHVTCNISSSVDEVTVPGAL 365

Query: 370 TLYIIEQ-----------------------------AEAEVKRLDQLKASKMKELFIKKQ 429
              +IEQ                             AE EV RLDQLKAS+MKE+  KKQ
Sbjct: 366 ARDLIEQVIYIALINLPMSSLRNQLLLANIHVKFVKAEVEVDRLDQLKASRMKEIAFKKQ 425

Query: 430 MELEDICNKSHMMIPSRSGMEKIINLIHSGEIDHANLLTSMDEQISRAKEEASSRKTIME 489
            ELE+I  ++H+ +   S  E+I++LI SG ++   LL  MD QIS+AKEEA SRK I++
Sbjct: 426 SELEEIYARAHVEVNPESARERIMSLIDSGNVEPTELLADMDSQISKAKEEAFSRKDILD 485

Query: 490 KVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNLRRAERARIAVNKIPALVELLLEKTR 549
           +VEKWM  C+EE WLE+YN DQ+RYS SRGAH NL+RAE+ARI V+KIPA+V+ L+ KTR
Sbjct: 486 RVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNLKRAEKARILVSKIPAMVDTLVAKTR 545

Query: 550 SWEAERSKVFLYDEVPLLELLEEYNALRQEK-EEKRRPREKKKVQTHTLIEPENFYGPRP 609
           +WE E S  F YD VPLL +L+EY  LRQE+ EEKRR RE+KKVQ    +E E+ +  RP
Sbjct: 546 AWEEEHSMSFAYDGVPLLAMLDEYGMLRQEREEEKRRLREQKKVQEQPHVEQESAFSTRP 571

Query: 610 S----TSSRRLSNASINGGYSNAIALNRRLSQGILQFGSNN 615
           S     S+++      N G +N    NRRLS    Q GS +
Sbjct: 606 SPARPVSAKKTVGPRANNGGANG-THNRRLSLNANQNGSRS 571

BLAST of Cp4.1LG11g03750 vs. TAIR 10
Match: AT4G26760.1 (microtubule-associated protein 65-2 )

HSP 1 Score: 407.5 bits (1046), Expect = 2.0e-113
Identity = 266/646 (41.18%), Postives = 377/646 (58.36%), Query Frame = 0

Query: 17  ETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELAE 76
           E +CG LLQ+LQ  W  +GE   +R+K+LL +E+ECL VY++KV+ A  SRA L Q L++
Sbjct: 13  EITCGTLLQKLQEIWDEVGESDEERDKLLLQIEEECLNVYKKKVELAAKSRAELLQTLSD 72

Query: 77  AEAEFTHLLLSLGERSLPGRPEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQVQI 136
           A  E ++L  +LGE+S    P+K +GT+KEQL +I PAL ++  +KEER++  S +Q QI
Sbjct: 73  ATVELSNLTTALGEKSYIDIPDKTSGTIKEQLSAIAPALEQLWQQKEERVRAFSDVQSQI 132

Query: 137 QKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYIDTV 196
           QKI  EI G   N       V ++TDLS+ +L+++Q +LQ L  EK++RLQ+V +++ TV
Sbjct: 133 QKICEEIAGGLNNGPH----VVDETDLSLKRLDDFQRKLQELQKEKSDRLQKVLEFVSTV 192

Query: 197 HKLSATLGMDASTIITKIHPSLDDPFG-RSKNISDSILEKLKGTVESLKEEKQQRLEKVM 256
           H L A L +D  + +T++HPSLD+  G ++K+IS+  L +L  TV +LKE+K QRL+K  
Sbjct: 193 HDLCAVLRLDFLSTVTEVHPSLDEANGVQTKSISNETLARLAKTVLTLKEDKMQRLKK-- 252

Query: 257 KLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNTVNKTIFD 316
                                                                       
Sbjct: 253 ------------------------------------------------------------ 312

Query: 317 KFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIEQ 376
                   L  L   LT+LW LMD+   +R  F HV   +S S  E++  G+L L +IEQ
Sbjct: 313 --------LQELATQLTDLWNLMDTSDEERELFDHVTSNISASVHEVTASGALALDLIEQ 372

Query: 377 AEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHSGEIDHAN 436
           AE EV RLDQLK+S+MKE+  KKQ ELE+I  ++H+ I      E+I++LI +G  +   
Sbjct: 373 AEVEVDRLDQLKSSRMKEIAFKKQSELEEIYARAHIEIKPEVVRERIMSLIDAGNTEPTE 432

Query: 437 LLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNLR 496
           LL  MD QI++AKEEA SRK I+++VEKWM  C+EE WLE+YN DQ+RYS SRGAH NL+
Sbjct: 433 LLADMDSQIAKAKEEAFSRKEILDRVEKWMSACEEESWLEDYNRDQNRYSASRGAHLNLK 492

Query: 497 RAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKE-EKR 556
           RAE+ARI V+KI A+V+ L+ KTR+WE E S  F YD VPLL +L+EY  LRQE+E EKR
Sbjct: 493 RAEKARILVSKITAMVDTLIAKTRAWEEENSMSFEYDGVPLLAMLDEYTMLRQEREDEKR 552

Query: 557 RPREKKKVQTHTLIEPENFYGPRPS----TSSRRLSNASINGGYSNAIALNRRLSQGILQ 616
           R +E+KK Q     + E+ +G +PS     S+++     +NGG  N   + RRLS    Q
Sbjct: 553 RLKEQKKQQEQPHTDQESAFGSKPSPARPVSAKKPVGTRVNGGGLNETPM-RRLSMNSNQ 569

Query: 617 FGSNNINSATQGISFIKEAKKSPGQNIARRHNLDSRVRDETASVVS 656
            GS              ++K+     IA   N+ +  +D+ AS VS
Sbjct: 613 NGS--------------KSKRDSLNKIASPSNIVANTKDDAASPVS 569

BLAST of Cp4.1LG11g03750 vs. TAIR 10
Match: AT5G51600.1 (Microtubule associated protein (MAP65/ASE1) family protein )

HSP 1 Score: 370.5 bits (950), Expect = 2.7e-102
Identity = 237/598 (39.63%), Postives = 346/598 (57.86%), Query Frame = 0

Query: 16  LETSCGYLLQELQ-AWIWMGEDQFDREKVLLDLEQECLEVYRRKVDDANVSRARLHQELA 75
           +ET+CG LL ELQ  W  +GE + DR+++LL+LE+ECLEVYRRKVD AN  RA+L Q +A
Sbjct: 12  VETTCGSLLFELQIIWDEVGETETDRDQMLLELERECLEVYRRKVDQANRCRAQLRQAIA 71

Query: 76  EAEAEFTHLLLSLGERSLPGR-PEKMAGTLKEQLDSITPALREMRLRKEERMKQLSAIQV 135
           +AEA+   +  ++GER +  R  ++  G+LK++L  I P L EM+ RK ER  Q   +  
Sbjct: 72  DAEAQLAAICSAMGERPVHIRQSDQSVGSLKQELGRILPELEEMQKRKVERRNQFIVVME 131

Query: 136 QIQKISAEIEGKAENVDSSRVVVENKTDLSMTKLEEYQNELQRLHSEKNERLQRVEKYID 195
           QI  I+ +I+G+ E V S  ++  ++T+LSM KLEE   +LQ L  EK +R++ + K++ 
Sbjct: 132 QIDSITNDIKGQGELVHSEPLI--DETNLSMRKLEELHCQLQVLQKEKIDRVETIRKHLC 191

Query: 196 TVHKLSATLGMDASTIITKIHPSLDDPFGRSKNISDSILEKLKGTVESLKEEKQQRLEKV 255
           T++   + LGMD + ++ +++P+L DP G  +++SD  +EKL   V+ L E K QR+++ 
Sbjct: 192 TLYSHCSVLGMDFNEVVGQVNPTLSDPEG-PRSLSDHTIEKLGAAVQKLMEVKIQRMQR- 251

Query: 256 MKLVLAKQHIYATSQNQKLYQKNKSHQNQVKPTVIVCLHLNKWLKSSNANFSNTVNKTIF 315
                                                                       
Sbjct: 252 ------------------------------------------------------------ 311

Query: 316 DKFLQCFLQLHNLGKTLTNLWELMDSPYGDRRSFSHVIRLLSFSSPEISDPGSLTLYIIE 375
                    L +L  T+  LW LMD+P  +++ + H+   ++ S  EI++  SL+   I+
Sbjct: 312 ---------LQDLATTMLELWNLMDTPIEEQQEYQHITCNIAASEHEITEANSLSEDFIK 371

Query: 376 QAEAEVKRLDQLKASKMKELFIKKQMELEDICNKSHMMIPSRSGMEKIINLIHSGEIDHA 435
             EAEV RLD++KASKMKEL +KK+ ELE+IC K+H++  S S +++ I  I SG +D  
Sbjct: 372 YVEAEVVRLDEVKASKMKELVLKKRSELEEICRKTHLLPVSDSAIDQTIVAIESGIVDAT 431

Query: 436 NLLTSMDEQISRAKEEASSRKTIMEKVEKWMLTCDEERWLEEYNMDQSRYSVSRGAHKNL 495
            +L  +++ IS+ KEEA SRK I+E+VEKW+  CDEE WLEEYN D +RY+  RGAH  L
Sbjct: 432 MVLEHLEQHISKIKEEALSRKEILERVEKWLSACDEESWLEEYNRDDNRYNAGRGAHLTL 491

Query: 496 RRAERARIAVNKIPALVELLLEKTRSWEAERSKVFLYDEVPLLELLEEYNALRQEKEEK- 555
           +RAE+AR  V K+P +VE L  KT  WE E    FLYD + LL +LEEYN LRQE+EE+ 
Sbjct: 492 KRAEKARNLVTKLPGMVEALASKTIVWEQENGIEFLYDGIRLLSMLEEYNILRQEREEEH 530

Query: 556 RRPREKKKVQTHTLIEPENFYGPRPSTS----SRRLSNASINGGYSNAIALNRRLSQG 607
           RR R++KK+Q   + E E  YG +PS S     ++    S  G      A NRRLS G
Sbjct: 552 RRQRDQKKLQGQLIAEQEALYGSKPSPSKPLGGKKAPRMSTGG------ASNRRLSLG 530