Cp4.1LG11g03500 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g03500
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAB hydrolase-1 domain-containing protein
LocationCp4.1LG11: 1894902 .. 1896933 (+)
RNA-Seq ExpressionCp4.1LG11g03500
SyntenyCp4.1LG11g03500
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCATAATTTGAAGAAAAATGGAATAAATATTATTAAAATTTTGGATTAGCATGGCATTAATAAAGGATATAATGGGCAATGTTTCTAACGTGGAATTGAAAAAGATTCGCCGATCCGCTCCTAAGAACTCACGCTATAATCGGCGCCGCCCAAATCAATTCAATGTGGCCGTCGTTTCTCAGCCTCGTATCCTTGTACGACGGGTATCTCCGCCGCTGCTTCACCGCCGCAGGCCTTACGCAGCAAGCCATCGACATCGACGCCGAAACGACTCTTTCATTTTGGGGCCCTAAGCTCAGTAAATCCAACGCGAAACCCTCCCTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCCACAAGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTTCCCGATCTCGTCTTCTTCGGCGGTTCGACGACCAAATCAGCGGAGAGGACTGAGGTTTTTCAGGCGATCTCGATCGGAAAGCTTCTGGAGAAGATCGGAGTGAAGAAATACTCGGTGATGGGAACGAGCTACGGCGGATTCGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAAGTTGTAATCGCGAGCTCCGGCGTTAATATGAGACGGAAAGATAACGACGCGATGTTGAAAAGAGCTAACGTGGAGAATATCGATGAATTTTTGCTGCCGATGACGGCGGAACAGTTACGGACGTTAATGAAGCTTGCTGTATTCAAGGGAGTGGGCAGACGGATGCCGGACTTCTTCTTGAACGATTTCATCCATGTAAGGACAAAATTCGTTTATATTATTTTATTATTATTTATTTATTTTCTTTTAATTATTTTTTTTTCTCTCATAAAAATATTATTAAAATTTAATAAGATATTTTATATATTTAAATAAAAAAACGAAAGGATTAATTATATTTATTAATTATTGAGGTAAATAATTATATAAATATAATTTATGATTTGGCTAGAATGTTTCTTAAACGTAATGGCGTGGGGTTCAGATCTAGAATCACGAAGCTATCGTGAACGCTTGAAATAACATACCAGTCAGCCAATTTAGATAATTTTAATCCCGAGAAATCATAGTAATTTTATATTTCGAGATATTATTTAAAGGTGAGATGGTGGTAAATAATTGTAGGTAAGAAAAACCTTCCCCATTATTTTTTCATTAATTTAAAGCTTTATTTATTAGGTGACGTCTGGTTTGACCTTTAAAAAAGACAAAATAAATAAATAAATATAAATATTGCAAATTGTGTATGCAGATAAATTGTTTTGATTTTTTTTTTTTTTTTTTGCTGAAATTTATTTATTTATTTATTCCATAGGATATATTTATAAATTCAGAAAATTAGTGTATGAAACGAATTAATTAATAATTTGTTTAAATAAATCCAGAAATTGTACATGGAAAAGAGGAAGGAAAAGATCGAACTCCTCAAGTCGCTCACGCTCGGGCGGGAAGATACCATCGACCTCTCTCCACTCTTTCAGGTAGGTCAAAGATTAGCTCGTTCGAGAGTGTCGTATTTTTATTAAAATTAACAATAAATTTGAAGTGGTTTATGTTCGGGATCGATATGCAGGAGGTATTGATAATATGGGGAGATCATGACCAGTTATTTCCCTTGGAAATGGCTAAGGAGCTGAAAGAGTGAGTTTTTTTATTTTTATTTTTATTTTTATTTTTTATTTTTTTGTTGAAATATTGGTGGGCTTCGGTTATAAATGTGTGGTGTAGGATTCTCGGGGAAAAGACAAGGCTGGAGGTGATGAAGGAGACCTCGCACGTGCCGCAGATTGAGGCTCCTGCAGAATTCAACCGTGTGGCCAAGAGTTTCTTGTGTGGGCTCTGATTTAATTATTTCCCATTTCCATATTTTTGGTGATTTTGTTTCTTTTTTTTTTTTCCTGAAATTTTTAATTTCATACAATTTAATTTTGCTTTTAATTTCTAAAATAAATAATTAACCCACTAATTTTAATAATAATTAAGTAAAATTCACT

mRNA sequence

AATCATAATTTGAAGAAAAATGGAATAAATATTATTAAAATTTTGGATTAGCATGGCATTAATAAAGGATATAATGGGCAATGTTTCTAACGTGGAATTGAAAAAGATTCGCCGATCCGCTCCTAAGAACTCACGCTATAATCGGCGCCGCCCAAATCAATTCAATGTGGCCGTCGTTTCTCAGCCTCGTATCCTTGTACGACGGGTATCTCCGCCGCTGCTTCACCGCCGCAGGCCTTACGCAGCAAGCCATCGACATCGACGCCGAAACGACTCTTTCATTTTGGGGCCCTAAGCTCAGTAAATCCAACGCGAAACCCTCCCTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCCACAAGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTTCCCGATCTCGTCTTCTTCGGCGGTTCGACGACCAAATCAGCGGAGAGGACTGAGGTTTTTCAGGCGATCTCGATCGGAAAGCTTCTGGAGAAGATCGGAGTGAAGAAATACTCGGTGATGGGAACGAGCTACGGCGGATTCGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAAGTTGTAATCGCGAGCTCCGGCGTTAATATGAGACGGAAAGATAACGACGCGATGTTGAAAAGAGCTAACGTGGAGAATATCGATGAATTTTTGCTGCCGATGACGGCGGAACAGTTACGGACGTTAATGAAGCTTGCTGTATTCAAGGGAGTGGGCAGACGGATGCCGGACTTCTTCTTGAACGATTTCATCCATAAATTGTACATGGAAAAGAGGAAGGAAAAGATCGAACTCCTCAAGTCGCTCACGCTCGGGCGGGAAGATACCATCGACCTCTCTCCACTCTTTCAGGAGGTATTGATAATATGGGGAGATCATGACCAGTTATTTCCCTTGGAAATGGCTAAGGAGCTGAAAGAGATTCTCGGGGAAAAGACAAGGCTGGAGGTGATGAAGGAGACCTCGCACGTGCCGCAGATTGAGGCTCCTGCAGAATTCAACCGTGTGGCCAAGAGTTTCTTGTGTGGGCTCTGATTTAATTATTTCCCATTTCCATATTTTTGGTGATTTTGTTTCTTTTTTTTTTTTCCTGAAATTTTTAATTTCATACAATTTAATTTTGCTTTTAATTTCTAAAATAAATAATTAACCCACTAATTTTAATAATAATTAAGTAAAATTCACT

Coding sequence (CDS)

ATGTGGCCGTCGTTTCTCAGCCTCGTATCCTTGTACGACGGGTATCTCCGCCGCTGCTTCACCGCCGCAGGCCTTACGCAGCAAGCCATCGACATCGACGCCGAAACGACTCTTTCATTTTGGGGCCCTAAGCTCAGTAAATCCAACGCGAAACCCTCCCTCCTCCTCCTCCACGGCTTCGGCCCGATGGCGATTTGGCAATGGCGTCCACAAGTCCAATTCCTCGCCGATGATTTCGACGTCTACGTTCCCGATCTCGTCTTCTTCGGCGGTTCGACGACCAAATCAGCGGAGAGGACTGAGGTTTTTCAGGCGATCTCGATCGGAAAGCTTCTGGAGAAGATCGGAGTGAAGAAATACTCGGTGATGGGAACGAGCTACGGCGGATTCGTGGCGTATCATATGGCGAGGATTTGGCCGGAGAGGATCGAGAAAGTTGTAATCGCGAGCTCCGGCGTTAATATGAGACGGAAAGATAACGACGCGATGTTGAAAAGAGCTAACGTGGAGAATATCGATGAATTTTTGCTGCCGATGACGGCGGAACAGTTACGGACGTTAATGAAGCTTGCTGTATTCAAGGGAGTGGGCAGACGGATGCCGGACTTCTTCTTGAACGATTTCATCCATAAATTGTACATGGAAAAGAGGAAGGAAAAGATCGAACTCCTCAAGTCGCTCACGCTCGGGCGGGAAGATACCATCGACCTCTCTCCACTCTTTCAGGAGGTATTGATAATATGGGGAGATCATGACCAGTTATTTCCCTTGGAAATGGCTAAGGAGCTGAAAGAGATTCTCGGGGAAAAGACAAGGCTGGAGGTGATGAAGGAGACCTCGCACGTGCCGCAGATTGAGGCTCCTGCAGAATTCAACCGTGTGGCCAAGAGTTTCTTGTGTGGGCTCTGA

Protein sequence

MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLCGL
Homology
BLAST of Cp4.1LG11g03500 vs. ExPASy Swiss-Prot
Match: Q6IE26 (Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2)

HSP 1 Score: 64.3 bits (155), Expect = 2.7e-09
Identity = 62/266 (23.31%), Postives = 114/266 (42.86%), Query Frame = 0

Query: 51  KPSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAI--SI 110
           KP +LLLHGF P   + WR Q++    ++ V   DL  +G S   + + +     +   I
Sbjct: 91  KPLMLLLHGF-PEFWYSWRHQLREFKSEYRVVALDLRGYGESDAPAHQESYKLDCLIADI 150

Query: 111 GKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIAS----SGVNMRRKDNDAML 170
             +L+ +G  K  ++G  +GG +A+ +A  +PE I K+++ +    S        + A L
Sbjct: 151 KDILDSLGYSKCVLIGHDWGGMIAWLIAVCYPEMIMKLIVINFPHPSVFTEYILRHPAQL 210

Query: 171 KRAN------VENIDEFLLPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRK 230
            R++      +    EF+  +   +    +  +   G+GR+       D    +Y+  + 
Sbjct: 211 FRSSFYYFFQIPRFPEFMFSINDFKALKHLFTSQSTGIGRKGRQLTTEDLEAYVYVFSQP 270

Query: 231 EKIELLKSLTLGREDTIDLSPLFQEV-----LIIWGDHDQLFPLEMAKELKEILGEKTRL 290
                L        +     PL   +     L++WG+ D    +EMA+  K  +    RL
Sbjct: 271 ---GALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGEEDAFMEVEMAEVTKIYVKNYFRL 330

Query: 291 EVMKETSHVPQIEAPAEFNRVAKSFL 300
            ++ E SH  Q + P   N +  +FL
Sbjct: 331 TILSEGSHWLQQDQPDIVNGLIWAFL 352

BLAST of Cp4.1LG11g03500 vs. ExPASy Swiss-Prot
Match: P19076 (2-hydroxymuconate semialdehyde hydrolase OS=Pseudomonas sp. (strain CF600) OX=79676 GN=dmpD PE=3 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.0e-08
Identity = 69/286 (24.13%), Postives = 128/286 (44.76%), Query Frame = 0

Query: 27  QQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGFGP-MAIW-QWRPQVQFLADDFDVYVP 86
           Q + +I  E   +     L  S A   L+++HG GP +  W  WR  +  LA    V  P
Sbjct: 5   QNSPEIGREIIAAGIRTNLHDSGAGFPLMMIHGSGPGVTAWANWRLVMPELAKSRRVIAP 64

Query: 87  DLVFFGGS--TTKSAERTEVFQAISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPER 146
           D++ FG S     +    +V+   ++G +L+ + +++  ++G S+GG +A  +A   PER
Sbjct: 65  DMLGFGYSERPADAQYNRDVWVDHAVG-VLDALEIEQADLVGNSFGGGIALALAIRHPER 124

Query: 147 IEKVVI-ASSGVNMRRKD--------NDAMLKRANVENIDEFLLPMTAEQLRTLMKLAVF 206
           + ++V+  S+GV+    +        N +  +   + +I  F   +  ++L  L   A  
Sbjct: 125 VRRLVLMGSAGVSFPITEGLDAVWGYNPSFAEMRRLLDIFAFDRNLVNDELAELRYQASI 184

Query: 207 KGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPLFQEVLIIWGDHDQ 266
           +      P F  ++    ++   R+  ++ L S          +  L  E L+I G  DQ
Sbjct: 185 R------PGF--HESFAAMFPAPRQRWVDGLASAEAA------IRALPHETLVIHGREDQ 244

Query: 267 LFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFL 300
           + PL+ +  L + +  + +L V  +  H  QIE  A F  +   FL
Sbjct: 245 IIPLQTSLTLADWIA-RAQLHVFGQCGHWTQIEHAARFASLVGDFL 274

BLAST of Cp4.1LG11g03500 vs. ExPASy Swiss-Prot
Match: Q8IUS5 (Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-08
Identity = 62/272 (22.79%), Postives = 116/272 (42.65%), Query Frame = 0

Query: 51  KPSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAI--SI 110
           KP +LLLHGF P   + WR Q++    ++ V   DL  +G +      +      +   I
Sbjct: 93  KPLMLLLHGF-PEFWYSWRYQLREFKSEYRVVALDLRGYGETDAPIHRQNYKLDCLITDI 152

Query: 111 GKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKD--------N 170
             +L+ +G  K  ++G  +GG +A+ +A  +PE + K+++    +N    +        +
Sbjct: 153 KDILDSLGYSKCVLIGHDWGGMIAWLIAICYPEMVMKLIV----INFPHPNVFTEYILRH 212

Query: 171 DAMLKRANVENIDEFLLPMTAEQLRTLMKLAVFK--------GVGRRMPDFFLNDFIHKL 230
            A L +++      F +P   E + ++    V K        G+GR+       D    +
Sbjct: 213 PAQLLKSSYYYF--FQIPWFPEFMFSINDFKVLKHLFTSHSTGIGRKGCQLTTEDLEAYI 272

Query: 231 YMEKRKEKIELLKSLTLGREDTIDLSPLFQEV-----LIIWGDHDQLFPLEMAKELKEIL 290
           Y+  +      L        +     PL   +     L++WG++D    +EMA+  K  +
Sbjct: 273 YVFSQP---GALSGPINHYRNIFSCLPLKHHMVTTPTLLLWGENDAFMEVEMAEVTKIYV 332

Query: 291 GEKTRLEVMKETSHVPQIEAPAEFNRVAKSFL 300
               RL ++ E SH  Q + P   N++  +FL
Sbjct: 333 KNYFRLTILSEASHWLQQDQPDIVNKLIWTFL 354

BLAST of Cp4.1LG11g03500 vs. ExPASy Swiss-Prot
Match: Q49KF8 (2-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase 1 OS=Pseudomonas putida OX=303 GN=mhpC1 PE=3 SV=2)

HSP 1 Score: 60.1 bits (144), Expect = 5.1e-08
Identity = 65/266 (24.44%), Postives = 116/266 (43.61%), Query Frame = 0

Query: 51  KPSLLLLHGFGPMAI-W-QWRPQVQFLAD-DFDVYVPDLVFF--GGSTTKSAERTEVFQA 110
           K ++++LHG GP A  W  +   ++ L +  F V + D   +    S   +  R+++   
Sbjct: 33  KETVVMLHGSGPGASGWANFNRNIEPLVNAGFRVILMDCPGWSKSDSIVSTGSRSDLNAR 92

Query: 111 ISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLK 170
           +  G L++K+ + K  ++G S GG  A   +  WPER+ K+V+   G           + 
Sbjct: 93  VLKG-LVDKLDLDKIHILGNSMGGHTAVAFSLTWPERVGKLVLMGGGTG--GVSPFVPMP 152

Query: 171 RANVENIDEFLLPMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLY------MEKRKE 230
              ++ ++      T E L+ +M + V+            +D   +L+      M  RK+
Sbjct: 153 SEGIKLLNGLYREPTIENLKKMMSIFVYD----------TSDLTEELFQTRLDNMLARKD 212

Query: 231 KIE-LLKSLTLGREDTID----LSPLFQEVLIIWGDHDQLFPLEMAKELKEILG-EKTRL 290
            +E    SL    +   D    L  +  E L+IWG +D+  PL+    L+ + G   ++L
Sbjct: 213 HLENFTASLAANLKQFPDFGHRLGEINAETLVIWGRNDRFVPLDTG--LRLVAGISNSQL 272

Query: 291 EVMKETSHVPQIEAPAEFNRVAKSFL 300
            V  +  H  Q E    FNR+   FL
Sbjct: 273 HVFNKCGHWAQWEHADTFNRMVLDFL 283

BLAST of Cp4.1LG11g03500 vs. ExPASy Swiss-Prot
Match: P9WNH2 (Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2788 PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 8.6e-08
Identity = 60/260 (23.08%), Postives = 111/260 (42.69%), Query Frame = 0

Query: 52  PSLLLLHGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKL 111
           P++LL+HG G  +   W      LA  F V  PDL+  G S    A+ +    A  +  L
Sbjct: 38  PAILLIHGIGDNST-TWNGVHAKLAQRFTVIAPDLLGHGQSDKPRADYSVAAYANGMRDL 97

Query: 112 LEKIGVKKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVEN 171
           L  + +++ +++G S GG VA   A  +P+ ++++++ S+G     KD + + + A++  
Sbjct: 98  LSVLDIERVTIVGHSLGGGVAMQFAYQFPQLVDRLILVSAG--GVTKDVNIVFRLASLPM 157

Query: 172 IDE--------FLLPMTAEQLRTLMKLAVFKGVGRRMPDF--FLNDFIHKLYMEKRKEKI 231
             E         +LP      R + K      +G  +P+    L+D             +
Sbjct: 158 GSEAMALLRLPLVLPAVQIAGRIVGKAIGTTSLGHDLPNVLRILDDLPEPTASAAFGRTL 217

Query: 232 ELLKSLTLGREDTIDLSPLFQ--EVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKET 291
             +          +D   L +   V IIWG  D + P+  A  +       ++LE+ + +
Sbjct: 218 RAVVDWRGQMVTMLDRCYLTEAIPVQIIWGTKDVVLPVRHA-HMAHAAMPGSQLEIFEGS 277

Query: 292 SHVPQIEAPAEFNRVAKSFL 300
            H P  + PA F  + + F+
Sbjct: 278 GHFPFHDDPARFIDIVERFM 293

BLAST of Cp4.1LG11g03500 vs. NCBI nr
Match: XP_023546569.1 (epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607 bits (1565), Expect = 4.56e-219
Identity = 302/302 (100.00%), Postives = 302/302 (100.00%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300

Query: 301 GL 302
           GL
Sbjct: 301 GL 302

BLAST of Cp4.1LG11g03500 vs. NCBI nr
Match: XP_022962163.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 596 bits (1536), Expect = 3.28e-214
Identity = 297/302 (98.34%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY GYLRRCFTAAGLTQQAIDIDAETTLSFWGPK SKSNAKPSLLLLHGF
Sbjct: 28  MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 87

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFG STTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 88  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 147

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 148 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 207

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLT GREDTIDLSPL
Sbjct: 208 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 267

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRV KSFLC
Sbjct: 268 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 327

Query: 301 GL 302
           GL
Sbjct: 328 GL 329

BLAST of Cp4.1LG11g03500 vs. NCBI nr
Match: KAG6598595.1 (Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 593 bits (1530), Expect = 9.88e-214
Identity = 295/302 (97.68%), Postives = 298/302 (98.68%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSF+SLVSLY GYLRRCFT+AGLTQQAI+IDAETTLSFWGPK SKSNAKPSLLLLHGF
Sbjct: 1   MWPSFVSLVSLYGGYLRRCFTSAGLTQQAIEIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFG STTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRV KSFLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 300

Query: 301 GL 302
           GL
Sbjct: 301 GL 302

BLAST of Cp4.1LG11g03500 vs. NCBI nr
Match: XP_022997555.1 (uncharacterized protein LOC111492447 [Cucurbita maxima])

HSP 1 Score: 592 bits (1526), Expect = 4.02e-213
Identity = 294/302 (97.35%), Postives = 296/302 (98.01%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY GYLRRCFTAAGLTQQAIDIDAETTLSFWGPK SKSNAKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGS TKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSMTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP IE+PAEFNRV K FLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPHIESPAEFNRVVKRFLC 300

Query: 301 GL 302
           G+
Sbjct: 301 GI 302

BLAST of Cp4.1LG11g03500 vs. NCBI nr
Match: XP_038885739.1 (monoacylglycerol lipase ABHD6-like isoform X1 [Benincasa hispida])

HSP 1 Score: 560 bits (1444), Expect = 1.27e-200
Identity = 271/301 (90.03%), Postives = 290/301 (96.35%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVS Y GYLRRCF+AAGL+ Q I+ID +TT++FWGPK +KS AKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSFYGGYLRRCFSAAGLSHQVINIDDQTTIAFWGPKPNKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWR QVQFLADDFDVYVPDLVFFGGSTTKSAERTE+FQA+S+GKL+EKIGVKKY
Sbjct: 61  GPMAIWQWRQQVQFLADDFDVYVPDLVFFGGSTTKSAERTEIFQAMSVGKLMEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SV+GTSYGGFVAYHMARIWPERIEKV+IASSGVNMRRKDNDAMLKRANVE IDEFLLP+T
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNDAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKR+EKIELLKSLTLGREDTI LSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKREEKIELLKSLTLGREDTISLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEI+GEKTRLEVMK+TSHVPQIEAPAEFNR+ K+FLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEIIGEKTRLEVMKDTSHVPQIEAPAEFNRLVKTFLC 300

BLAST of Cp4.1LG11g03500 vs. ExPASy TrEMBL
Match: A0A6J1HE07 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462697 PE=4 SV=1)

HSP 1 Score: 596 bits (1536), Expect = 1.59e-214
Identity = 297/302 (98.34%), Postives = 297/302 (98.34%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY GYLRRCFTAAGLTQQAIDIDAETTLSFWGPK SKSNAKPSLLLLHGF
Sbjct: 28  MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 87

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFG STTKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 88  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGDSTTKSAERTEVFQAISIGKLLEKIGVKKY 147

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 148 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 207

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLT GREDTIDLSPL
Sbjct: 208 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTFGREDTIDLSPL 267

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRV KSFLC
Sbjct: 268 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVVKSFLC 327

Query: 301 GL 302
           GL
Sbjct: 328 GL 329

BLAST of Cp4.1LG11g03500 vs. ExPASy TrEMBL
Match: A0A6J1KA03 (uncharacterized protein LOC111492447 OS=Cucurbita maxima OX=3661 GN=LOC111492447 PE=4 SV=1)

HSP 1 Score: 592 bits (1526), Expect = 1.95e-213
Identity = 294/302 (97.35%), Postives = 296/302 (98.01%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY GYLRRCFTAAGLTQQAIDIDAETTLSFWGPK SKSNAKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKPSKSNAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGS TKSAERTEVFQAISIGKLLEKIGVKKY
Sbjct: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSMTKSAERTEVFQAISIGKLLEKIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT
Sbjct: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL
Sbjct: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
           FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVP IE+PAEFNRV K FLC
Sbjct: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPHIESPAEFNRVVKRFLC 300

Query: 301 GL 302
           G+
Sbjct: 301 GI 302

BLAST of Cp4.1LG11g03500 vs. ExPASy TrEMBL
Match: A0A5D3BNV7 (Monoacylglycerol lipase ABHD6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold187G00070 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 8.87e-192
Identity = 262/301 (87.04%), Postives = 283/301 (94.02%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY  YLRRCF+AAGL+QQ I ID ETT+SFWGPK  KS AKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGRYLRRCFSAAGLSQQVIHIDDETTISFWGPKPHKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GP AIWQWR QVQFLADDFD+YVPDLVFFGGSTTKSAERTE+FQA+SIGKL+E IGVKKY
Sbjct: 61  GPAAIWQWRQQVQFLADDFDLYVPDLVFFGGSTTKSAERTEIFQAMSIGKLVEMIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SV+GTSYGGFVAYHMARIWPERIEKV+IASSGVNMRRKDN+AMLKRANVE IDEFLLP+T
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKG GR+MPDFF NDFIHKLYME R++KIELLKSLTLGRED+I+LSPL
Sbjct: 181 AEQLRTLMKLAVFKGGGRKMPDFFFNDFIHKLYMENREQKIELLKSLTLGREDSINLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
            QEVLIIWGDHDQLFPLEMAKELK I+GEKTRLEV+KETSHVPQIEAPA+FN++ KSFLC
Sbjct: 241 SQEVLIIWGDHDQLFPLEMAKELKGIIGEKTRLEVLKETSHVPQIEAPAQFNQLVKSFLC 300

BLAST of Cp4.1LG11g03500 vs. ExPASy TrEMBL
Match: A0A1S3BDU7 (monoacylglycerol lipase ABHD6-like OS=Cucumis melo OX=3656 GN=LOC103488527 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 8.87e-192
Identity = 262/301 (87.04%), Postives = 283/301 (94.02%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGF 60
           MWPSFLSLVSLY  YLRRCF+AAGL+QQ I ID ETT+SFWGPK  KS AKPSLLLLHGF
Sbjct: 1   MWPSFLSLVSLYGRYLRRCFSAAGLSQQVIHIDDETTISFWGPKPHKSTAKPSLLLLHGF 60

Query: 61  GPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKY 120
           GP AIWQWR QVQFLADDFD+YVPDLVFFGGSTTKSAERTE+FQA+SIGKL+E IGVKKY
Sbjct: 61  GPAAIWQWRQQVQFLADDFDLYVPDLVFFGGSTTKSAERTEIFQAMSIGKLVEMIGVKKY 120

Query: 121 SVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPMT 180
           SV+GTSYGGFVAYHMARIWPERIEKV+IASSGVNMRRKDN+AMLKRANVE IDEFLLP+T
Sbjct: 121 SVLGTSYGGFVAYHMARIWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDEFLLPVT 180

Query: 181 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 240
           AEQLRTLMKLAVFKG GR+MPDFF NDFIHKLYME R++KIELLKSLTLGRED+I+LSPL
Sbjct: 181 AEQLRTLMKLAVFKGGGRKMPDFFFNDFIHKLYMENREQKIELLKSLTLGREDSINLSPL 240

Query: 241 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 300
            QEVLIIWGDHDQLFPLEMAKELK I+GEKTRLEV+KETSHVPQIEAPA+FN++ KSFLC
Sbjct: 241 SQEVLIIWGDHDQLFPLEMAKELKGIIGEKTRLEVLKETSHVPQIEAPAQFNQLVKSFLC 300

BLAST of Cp4.1LG11g03500 vs. ExPASy TrEMBL
Match: A0A6J1BQG4 (uncharacterized protein LOC111004859 OS=Momordica charantia OX=3673 GN=LOC111004859 PE=4 SV=1)

HSP 1 Score: 537 bits (1383), Expect = 1.41e-191
Identity = 263/303 (86.80%), Postives = 286/303 (94.39%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKL--SKSNAKPSLLLLH 60
           MWPSFLSLVSLY GYLRRCFTAAGL+ QA++ID ETT++FWGPK+  +K   K SL+LLH
Sbjct: 1   MWPSFLSLVSLYGGYLRRCFTAAGLSGQAVEIDNETTIAFWGPKIITNKPTGKRSLVLLH 60

Query: 61  GFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVK 120
           GFGPMAIWQWR QVQFLADDFDVYVPDLVFFGGSTTKS+ERTEVFQAIS+GKLLEKIGVK
Sbjct: 61  GFGPMAIWQWRKQVQFLADDFDVYVPDLVFFGGSTTKSSERTEVFQAISVGKLLEKIGVK 120

Query: 121 KYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLP 180
           KYSVMGTSYGGFVAYHMAR WPERIEKV+IASSGVNMRRKDN+AMLKRANVE ID+FLLP
Sbjct: 121 KYSVMGTSYGGFVAYHMARFWPERIEKVIIASSGVNMRRKDNEAMLKRANVEKIDDFLLP 180

Query: 181 MTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLS 240
           +TAEQLRTLMKLAVFK VGRR+PDFFLNDFIHKLYME R+EKIELLKSLT+GREDT++LS
Sbjct: 181 VTAEQLRTLMKLAVFKEVGRRLPDFFLNDFIHKLYMENREEKIELLKSLTIGREDTLNLS 240

Query: 241 PLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSF 300
           PL +EVLIIWGDHDQLFPL+MAKELKEILGEKTRLEVMKETSHVPQIE+PAEFNR+ KSF
Sbjct: 241 PLSEEVLIIWGDHDQLFPLQMAKELKEILGEKTRLEVMKETSHVPQIESPAEFNRLVKSF 300

BLAST of Cp4.1LG11g03500 vs. TAIR 10
Match: AT4G33180.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 349.7 bits (896), Expect = 2.3e-96
Identity = 173/302 (57.28%), Postives = 221/302 (73.18%), Query Frame = 0

Query: 4   SFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGP---KLSKSNAKPSLLLLHGF 63
           S+LS   L +GYLRRC  AAGLT Q + ID+ETT+ FWGP        + +P +LLLHGF
Sbjct: 5   SYLSPTRLLEGYLRRCLRAAGLTSQTLSIDSETTIHFWGPPPLDHRSDDDRPVMLLLHGF 64

Query: 64  GPMAIWQWRPQVQ-FLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKK 123
           GP ++WQWR Q+Q F    F VY PDLVFFG ST+ S  RTEVFQA  + KL+ KIG+ K
Sbjct: 65  GPSSMWQWRRQMQAFSPSAFRVYSPDLVFFGDSTSSSTNRTEVFQAECMAKLMAKIGIGK 124

Query: 124 YSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLLPM 183
           Y+V GTSYGGFVAYHMA++WPE++EKVVIASSG+NMR+ D +++L+R+N E I++ +LP 
Sbjct: 125 YNVAGTSYGGFVAYHMAKMWPEKVEKVVIASSGINMRKCDGESLLQRSNCECIEKVMLPS 184

Query: 184 TAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSP 243
           TA + RTLM LA    + R  PD   ND I+ LY + RKEKIELLK +T GR + +++  
Sbjct: 185 TATEFRTLMALASSWRLVRMFPDALWNDVINNLYKKNRKEKIELLKGVTFGRSENLNIDS 244

Query: 244 LFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFL 302
           L QEVLI+WGD DQ+FP++MA ELKEILG+KT+LE++  TSHVPQIE   EFN +   FL
Sbjct: 245 LSQEVLIVWGDKDQIFPVKMAYELKEILGDKTKLEIIDNTSHVPQIECAQEFNNIVLRFL 304

BLAST of Cp4.1LG11g03500 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 3.8e-91
Identity = 162/302 (53.64%), Postives = 225/302 (74.50%), Query Frame = 0

Query: 1   MWPSFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSN---AKPSLLLL 60
           M PSFLS+    +  LRR F++AGL+ Q + ID+ETT+ FWGP  S S+    KPSLLLL
Sbjct: 1   MAPSFLSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLL 60

Query: 61  HGFGPMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGV 120
           HGFGP A+WQW  QV+ L+  F +YVPDLVFFGGS++    R+E+FQA+ +GKL+EK+ V
Sbjct: 61  HGFGPSAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEV 120

Query: 121 KKYSVMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDNDAMLKRANVENIDEFLL 180
           +++SV+GTSYGGFVAY+MA+++PE++EKVV+ASSGVN+RR DN+A + RA    I E +L
Sbjct: 121 ERFSVVGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVML 180

Query: 181 PMTAEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDL 240
           P +A  LR    +   K +   +PDF LNDF  K+Y EKR+EK ELL+ L++G++D  ++
Sbjct: 181 PASATDLRRFSGMVSSKRLD-YVPDFVLNDFCQKMYSEKREEKAELLEGLSIGKDDKTNV 240

Query: 241 SPLFQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKS 300
           SP+ Q+V++IWG+ DQ+FPL+MA +LKE+LG K  L+V+++TSH+PQ E   EFN    S
Sbjct: 241 SPIQQDVMLIWGEQDQVFPLKMAHDLKEMLGTKATLKVIQKTSHIPQTEKSKEFNGFVMS 300

BLAST of Cp4.1LG11g03500 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 215.3 bits (547), Expect = 6.7e-56
Identity = 117/284 (41.20%), Postives = 180/284 (63.38%), Query Frame = 0

Query: 20  FTAAGLTQQAIDIDAETTLSFWGPKLS-KSNAKPSLLLLHGFGPMAIWQWRPQVQFLADD 79
           F  +GL    ID+   T ++FW  K   +S  KP+LLL+HG G  AIWQW    + L+  
Sbjct: 19  FKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDVARRLSRY 78

Query: 80  FDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKYSVMGTSYGGFVAYHMARI 139
           F++Y+PDLVFFGGS+T   ER+++FQA ++ + LE   VKK+S++G SYGGFV Y MA +
Sbjct: 79  FNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKKFSLVGLSYGGFVGYRMASM 138

Query: 140 WPERIEKVVIASSGVNMRRKDNDA-MLKRANVENIDEFLLPMTAEQLRTLMKLAVFK-GV 199
           + + +EKVVI  + V +  KD  A + K ++++   + L+P + ++LR LM    +K  +
Sbjct: 139 YADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMGYIFYKPAL 198

Query: 200 GRRMPDFFLNDFI-HKLYMEKRKEKIELLKSLTLGREDTIDLSPLFQEVLIIWGDHDQLF 259
            R +P   L+DFI H L  +  +EK EL+K++   R  + ++  L Q  LIIWG+HDQ+F
Sbjct: 199 ARLVPTCLLHDFIEHALTRDNMEEKRELIKAIPKDRIIS-EIPKLKQPTLIIWGEHDQVF 258

Query: 260 PLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFL 300
           PLEM K L++ +G+  +L ++K T H+   E P +F ++ KSFL
Sbjct: 259 PLEMGKRLEKHVGDNGKLVIIKRTGHIFNFEKPKKFIKLLKSFL 301

BLAST of Cp4.1LG11g03500 vs. TAIR 10
Match: AT5G09430.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 211.1 bits (536), Expect = 1.3e-54
Identity = 120/299 (40.13%), Postives = 176/299 (58.86%), Query Frame = 0

Query: 7   SLVSLYDGYLRRCFTAAGLTQQAIDID-----AETTLSFWGPKLSKSNAKPSLLLLHGFG 66
           S  +  D   R+ F  AGL     D+      A T +  W PK S + +KP+LLLLHGFG
Sbjct: 12  SFTASRDWLFRQSFANAGLRSVTTDLSHGNSIASTAMHCWIPK-SPNRSKPNLLLLHGFG 71

Query: 67  PMAIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKYS 126
             A+WQ+   ++     F+VYVPDL+FFG S+T    RTE FQA  + +L+E  GV++ +
Sbjct: 72  ANAMWQYGEHLRAFTGRFNVYVPDLLFFGLSSTSEPNRTESFQARCLMRLMEAHGVQRMN 131

Query: 127 VMGTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKD-NDAMLKRANVENIDEFLLPMT 186
           ++G SYGGFV Y +A  +PE +EK+V+  +GV +  KD  D + K  N+E     L+P T
Sbjct: 132 IVGISYGGFVGYSLAAQFPENVEKLVLCCAGVCLEEKDMEDGLFKVPNLEEATGILIPQT 191

Query: 187 AEQLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPL 246
            E+L+ L++ +  K + + +P FFL DFI  +  E  +EK +L+KS+   R    DL  +
Sbjct: 192 PEKLKELIRFSFVKPI-KGVPSFFLWDFIDVMCTEFVEEKRDLIKSILKDRR-LSDLPRI 251

Query: 247 FQEVLIIWGDHDQLFPLEMAKELKEILGEKTRLEVMKETSHVPQIEAPAEFNRVAKSFL 300
            Q+ LIIWG+ DQ+FPLE+   LK  +GE   + V+K+  H   +E   EF +  KSFL
Sbjct: 252 KQKSLIIWGEEDQIFPLELGYRLKRHIGESAEIVVIKKAGHAVNLEKSKEFVKHLKSFL 307

BLAST of Cp4.1LG11g03500 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 196.8 bits (499), Expect = 2.5e-50
Identity = 116/299 (38.80%), Postives = 171/299 (57.19%), Query Frame = 0

Query: 4   SFLSLVSLYDGYLRRCFTAAGLTQQAIDIDAETTLSFWGPKLSKSNAKPSLLLLHGFGPM 63
           S  S VS  +   +  F+ AGL     D+   T    W P L+  + KP+LLLLHG G  
Sbjct: 3   SCFSYVSSRNKCYQYSFSRAGLRSSTSDLGDGTVFHCWIP-LTHIHTKPTLLLLHGIGAN 62

Query: 64  AIWQWRPQVQFLADDFDVYVPDLVFFGGSTTKSAERTEVFQAISIGKLLEKIGVKKYSVM 123
           A+WQW   +      F+VYVPDL+FFG S T   +R+E FQA  + K ++  GV+  +V 
Sbjct: 63  AMWQWDRFIDRFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGVRTMTVA 122

Query: 124 GTSYGGFVAYHMARIWPERIEKVVIASSGVNMRRKDN-DAMLKRANVENIDEFLLPMTAE 183
           G SYGGFVAY +A  + ER+++VV+  +GV +  KD+ D M K  + E     L P +  
Sbjct: 123 GLSYGGFVAYSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPS 182

Query: 184 QLRTLMKLAVFKGVGRRMPDFFLNDFIHKLYMEKRKEKIELLKSLTLGREDTIDLSPLFQ 243
            LR L++L+ +K     +P  F  D+IH +  +  +E+ EL+++L  GR    +L  + Q
Sbjct: 183 MLRRLLQLSFYK-PPIWIPSCFAMDYIHVMCKDYLQERKELVEALHKGRR-FANLPKITQ 242

Query: 244 EVLIIWGDHDQLFPLEMAKELKEILGE-KTRLEVMKETSHVPQIEAPAEFNRVAKSFLC 301
             L+IWG+ DQ+FP+E+A  LK  LGE + +L ++K+T H    E P E  +  KSFLC
Sbjct: 243 PTLMIWGEEDQVFPVELAHRLKRYLGEDRAQLVLLKKTGHAINEEKPKEMYKHMKSFLC 298

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6IE262.7e-0923.31Epoxide hydrolase 4 OS=Mus musculus OX=10090 GN=Ephx4 PE=2 SV=2[more]
P190761.0e-0824.132-hydroxymuconate semialdehyde hydrolase OS=Pseudomonas sp. (strain CF600) OX=79... [more]
Q8IUS51.3e-0822.79Epoxide hydrolase 4 OS=Homo sapiens OX=9606 GN=EPHX4 PE=1 SV=2[more]
Q49KF85.1e-0824.442-hydroxy-6-oxononadienedioate/2-hydroxy-6-oxononatrienedioate hydrolase 1 OS=Ps... [more]
P9WNH28.6e-0823.08Uncharacterized protein MT2788 OS=Mycobacterium tuberculosis (strain CDC 1551 / ... [more]
Match NameE-valueIdentityDescription
XP_023546569.14.56e-219100.00epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo][more]
XP_022962163.13.28e-21498.34epoxide hydrolase 4-like [Cucurbita moschata][more]
KAG6598595.19.88e-21497.68Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022997555.14.02e-21397.35uncharacterized protein LOC111492447 [Cucurbita maxima][more]
XP_038885739.11.27e-20090.03monoacylglycerol lipase ABHD6-like isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1HE071.59e-21498.34epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111462697 PE=4 SV=1[more]
A0A6J1KA031.95e-21397.35uncharacterized protein LOC111492447 OS=Cucurbita maxima OX=3661 GN=LOC111492447... [more]
A0A5D3BNV78.87e-19287.04Monoacylglycerol lipase ABHD6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3BDU78.87e-19287.04monoacylglycerol lipase ABHD6-like OS=Cucumis melo OX=3656 GN=LOC103488527 PE=4 ... [more]
A0A6J1BQG41.41e-19186.80uncharacterized protein LOC111004859 OS=Momordica charantia OX=3673 GN=LOC111004... [more]
Match NameE-valueIdentityDescription
AT4G33180.12.3e-9657.28alpha/beta-Hydrolases superfamily protein [more]
AT5G21950.13.8e-9153.64alpha/beta-Hydrolases superfamily protein [more]
AT1G78210.16.7e-5641.20alpha/beta-Hydrolases superfamily protein [more]
AT5G09430.11.3e-5440.13alpha/beta-Hydrolases superfamily protein [more]
AT4G39955.12.5e-5038.80alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000073Alpha/beta hydrolase fold-1PRINTSPR00111ABHYDROLASEcoord: 136..149
score: 32.51
coord: 78..93
score: 35.99
coord: 244..258
score: 42.99
coord: 122..135
score: 34.48
IPR000073Alpha/beta hydrolase fold-1PFAMPF12697Abhydrolase_6coord: 54..293
e-value: 6.0E-14
score: 53.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 15..300
e-value: 1.8E-43
score: 151.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 40..299
NoneNo IPR availablePANTHERPTHR43139SI:DKEY-122A22.2coord: 5..300
NoneNo IPR availablePANTHERPTHR43139:SF52ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEINcoord: 5..300

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g03500.1Cp4.1LG11g03500.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity