Cp4.1LG11g01020 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g01020
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionSqualene monooxygenase
LocationCp4.1LG11: 575817 .. 580114 (-)
RNA-Seq ExpressionCp4.1LG11g01020
SyntenyCp4.1LG11g01020
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGGGAGTTGGGAGATAATTGAGAGTAATTTAGGAGAGATGCGGATGTTAAGCGGCGCAAGTTAGTATTAGGCGCCACATTTGAGCTGTAACTAATTATATCCTATGCGACTGTTCATTCAAGGAGACGGGACGGCACCAATGTATAATGTGGCTTCCTTTTCACCACACACATTCTATAAAATCATCCCCTCTTCCTCAATTTTCACTCCCCTTAACCGAATCTTCTAGACTGACCGATAATCTTTCGTCGTCGTCGTCGTCGTCGCCGCTGCGGTGATCTTCGTGTGGTTGCTGGGATTTTACATTCTCTGGAACGAGGAAACAATGGTTGATTACTGCGCCTTTGGCTGGATCTTGGCCGCCGTCCTCGGCCTTGCCATTGCACTCTCTTTCTTCGTCTCTCCTAGGAGGAATCGTCGAGGAGGAGCCGATTCCACGCCGAGGAGCGAGGGTGTCATAAGCTCTTCAGCCACCAACGGAGAATGCAGATCCGTCGACGGTGACGCCGACGTTATCATCGTCGGAGCAGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGTACATCGGTTTTATTGCTTTCTTTTGAATTTTGAATCTGTTCTGTACTTCTGTTCGCTCTCTCTCTCTCTCTCTCTGCATTTTTCTCCGTCCTGTTCGCGCCACCACGCATCTAATTACTCCTTCTGGCCCTTATTATTAATGAAAACTGGGTATTTGCGTGCTGTTTATATTTGATGCATAGAAACCCTCAGAATGAAAGAATCTATCGCAAAGTTATGGATTATGGATGAGAGGAAGAAAAATGGAAAGAAGAAAGATATTACTTCGGGATGTCAATTGCTTGTCCATGTAACCAATGAAATGACTGGATGATCGTTTTTCTGTCGTTTTCCCCCTTCTAATTTGTTATTGGATGAAAGTTGTATGTCGCCACCAGATGATTTTTATATGGATAAATGGAGTGCTTCAATTTCGTTGTAAAATGGTTTTGATTATTGCTGACTTAAGCAGAAGGTTCGAATGTATATTTTAAATTTTAACCCATCTATATAATTGTTGTTCGATCTTCGGTGAATAACCATATGAATTTCCTTTTGGTAAAGAAGTTAATTATTGGGGTTGGATTGTTTATATCCACATCCCTCTTCTTATTGGTTTTATTTATTTATTTTTTTTCTATTTTTGTTGTTCCTCATATTTCACTTTGGAACCAAGCTGATCATATCGAATTTGATAGGATGGTCGTCTAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGGTTTGTAATTGGGCTGGGGAAATTTCATTTACAACTCTATTTTCTTTCGAAGTACAATATTCATCAGTAATTAAGGAAAATACATGTTTGTATTGCAGATTGTGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAACACTCAACTTTCTTACCCATTGGAAAAATTTCAGTCTGATGTATCTGGACGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCCCTTCCCAAGTATGTTCATTTCGTCTTTGTTTTCCGCACCTTGAATTTTTAAGAGTATGAGTTAAATAACCCCCTGTAGTGCTGACTGTTGAAGGTGTTACGAATGTCCCATGGCCCGATCTTTTAAAGTAATGATTCAAGCCATATTTTATATTTCCTTAATTCCGTTTTGTGAACTGAGTAAGAATTTTTGTTTAGACGATTCATGCACTTCCTCTTAGTTGTTCTGTGTATGAAACTTGTGCTGTTGAAACGTTAGAGATTTGACCATATCGTTAAGCAGACTCGGACAATATGACGGTGAAATTCATGTTATTTTTACATAAATAAATGGATTTAAGAAAATTTTCTTAAGTTTGGTTTACAGCTGCCATAACCAACCGTTAGCAGTATTGTCCTCTTTCCACTTTTCTTTTCGGCCTTTTTCGCAAAGTTTTTAAAATGCGTCTGCGAATGAGAGGTTTCCACTCTTATAAGAAATGCTTCGTTTCTCTCTCCAACCGATGTAGAATCTTACACTCCACTCCCTTGGGAGCCCAATGTCTTCGCTGGCACACTACCCAAGGTCTAGCTCTGATACCATTTGTAACAGTCCATGCCCCTCTAGCAGCCATTATTCTCTTGGGCTTTCCTAGTTTCTCCTCAAGATTTTTAAAACGCGTAGGGAGAAGTTTTCACATTCTTGTAAGGAATGCTTTGCATGAATCTAGTTAAAGTCTCTAGAGAGTTCAACAATGGCCATTGATTCACCATTGATACTCCAAGTCTGTGTTTCTTGTGATTGGATAATGAATAAATACTTGGATTTTACTCATCAATGTGACTTTTTATTTGCTCTGTTTCCATTTCTTGTAGTGTAAGATTGGAGCAAGGAACAGTTACTTCGTTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAGTCTAAAAATGGTGAAGAAAAAACAGCGTATGCACCTCTGACCATTGTTTGTGATGGGTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAGCCTATGGTAAGGAGGCTTCAATTATTCAGTGGTACCTCAAAATAGACATTTTCTCTTTAGGATTTGATTGGCTGCAGAATTATTCTAATTTCATTTTGCTAACTGCATGAAATTATCGAATACGTTCTTCAAATTCCGAGCCCTTTATCATCTCTATCTTTCGCAGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGACATGTTGTTCTCGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGTAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCATAGTAGCTCCTCAGGTACAATTTTTCAACTGTCCCATTCATGCCAACTTCCATCTTAACATTTATGTGTTAAATTTAGTGCTTCGAACTAATGAATTACCATTACTCGTGCAGGTTCCTCCGCAAATCCATGATGCCTTTATAGCTGCTATTGACAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCTGCTCCCCAACCGACGCCCGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCCCTCACCGGTGGAGGAATGACAGTTGCTTTGTCTGATATTGTTGTGCTCAGGAACCTTCTCAAGCCACTGAAGGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGCGAAAGGTATAATTTTAGTTTATGTTTGCTTTTTCCTATAAGCAATTTCACAGAAGGCCATTAGTAAAGCTAATTTATTCGTCTCAAACTGCAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTCTTTTGTGCATCGCCTGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTGTCCTTGCTCTCGGGATTGAATCCTCGCCCATTAAGTCTGGTTCTCCATTTCTTTGCAGTGGCAATATACGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATGTGAGTTATGAACAGACAAAACAGAACAAAACACCTTCCATTCATATTTTCCTTTGAAAACTTTAAGAACTTCGTAGGGAAAGTGTTGTAATGAGAAATGAATTGATTTGTTTGCAGAGCGCATCAGGAATCATATTTCCGATTATCAAGGCGGAAGGAGTTAGACAGATGTTCTTCCCTGCTACTGTTCCTGCTTATTACAGAAGCCCACCAGTTCTTAAAACCATAGCATGAAGACTGGGTATTTGTTTGTGTCATACCTGAGGAGGAAGAAGAGGCATCCTCAGGCTGTCGAGCATTGCAGGGAAGACAAATCACTTTTTCGTTTGTTAGATTTTCATTTTAATTGTGGATTAATAAACCTGTCTAACTTTTGAACAGGAGCCAGGAATATGTAAATGTTCTTAATTCGTTCTAGAAACCCCAACATTAATGTATTTTCGAACAGCTTGGGAACTTTCAACAGAGTAATCCCTAAAACAATCAATTTAGTACTCACCATTACCTTGTCATTTATATCATTCGAAAAT

mRNA sequence

TGGGAGTTGGGAGATAATTGAGAGTAATTTAGGAGAGATGCGGATGTTAAGCGGCGCAAGTTAGTATTAGGCGCCACATTTGAGCTGTAACTAATTATATCCTATGCGACTGTTCATTCAAGGAGACGGGACGGCACCAATGTATAATGTGGCTTCCTTTTCACCACACACATTCTATAAAATCATCCCCTCTTCCTCAATTTTCACTCCCCTTAACCGAATCTTCTAGACTGACCGATAATCTTTCGTCGTCGTCGTCGTCGTCGCCGCTGCGGTGATCTTCGTGTGGTTGCTGGGATTTTACATTCTCTGGAACGAGGAAACAATGGTTGATTACTGCGCCTTTGGCTGGATCTTGGCCGCCGTCCTCGGCCTTGCCATTGCACTCTCTTTCTTCGTCTCTCCTAGGAGGAATCGTCGAGGAGGAGCCGATTCCACGCCGAGGAGCGAGGGTGTCATAAGCTCTTCAGCCACCAACGGAGAATGCAGATCCGTCGACGGTGACGCCGACGTTATCATCGTCGGAGCAGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCTAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGATTGTGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAACACTCAACTTTCTTACCCATTGGAAAAATTTCAGTCTGATGTATCTGGACGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCCCTTCCCAATGTAAGATTGGAGCAAGGAACAGTTACTTCGTTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAGTCTAAAAATGGTGAAGAAAAAACAGCGTATGCACCTCTGACCATTGTTTGTGATGGGTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAGCCTATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGACATGTTGTTCTCGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGTAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCATAGTAGCTCCTCAGGTTCCTCCGCAAATCCATGATGCCTTTATAGCTGCTATTGACAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCTGCTCCCCAACCGACGCCCGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCCCTCACCGGTGGAGGAATGACAGTTGCTTTGTCTGATATTGTTGTGCTCAGGAACCTTCTCAAGCCACTGAAGGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGCGAAAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTCTTTTGTGCATCGCCTGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTGTCCTTGCTCTCGGGATTGAATCCTCGCCCATTAAGTCTGGTTCTCCATTTCTTTGCAGTGGCAATATACGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCGATTATCAAGGCGGAAGGAGTTAGACAGATGTTCTTCCCTGCTACTGTTCCTGCTTATTACAGAAGCCCACCAGTTCTTAAAACCATAGCATGAAGACTGGGTATTTGTTTGTGTCATACCTGAGGAGGAAGAAGAGGCATCCTCAGGCTGTCGAGCATTGCAGGGAAGACAAATCACTTTTTCGTTTGTTAGATTTTCATTTTAATTGTGGATTAATAAACCTGTCTAACTTTTGAACAGGAGCCAGGAATATGTAAATGTTCTTAATTCGTTCTAGAAACCCCAACATTAATGTATTTTCGAACAGCTTGGGAACTTTCAACAGAGTAATCCCTAAAACAATCAATTTAGTACTCACCATTACCTTGTCATTTATATCATTCGAAAAT

Coding sequence (CDS)

ATGGTTGATTACTGCGCCTTTGGCTGGATCTTGGCCGCCGTCCTCGGCCTTGCCATTGCACTCTCTTTCTTCGTCTCTCCTAGGAGGAATCGTCGAGGAGGAGCCGATTCCACGCCGAGGAGCGAGGGTGTCATAAGCTCTTCAGCCACCAACGGAGAATGCAGATCCGTCGACGGTGACGCCGACGTTATCATCGTCGGAGCAGGTGTTGCCGGTTCCGCTCTTGCTCACACTCTCGGCAAGGATGGTCGTCTAGTTCACGTGATTGAAAGAGACTTGACAGAGCCTGATAGAATCGTCGGTGAATTATTGCAGCCTGGGGGCTACCTCAAATTGATTGAGTTAGGACTGCAAGATTGTGTTGAGGAAATTGATGCACAGAAGGTGTATGGCTATGCCCTTTTCAAGGATGGAAAGAACACTCAACTTTCTTACCCATTGGAAAAATTTCAGTCTGATGTATCTGGACGAAGCTTCCACAATGGACGCTTCATACAGAGAATGAGGGAGAAGGCTGCTTCCCTTCCCAATGTAAGATTGGAGCAAGGAACAGTTACTTCGTTGCTTGAAGAAAAGGGAACGATCAAAGGTGTGCAGTATAAGTCTAAAAATGGTGAAGAAAAAACAGCGTATGCACCTCTGACCATTGTTTGTGATGGGTGCTTCTCAAATTTGCGCCGCTCTCTTTGCAAGCCTATGGTTGATGTTCCATCTTGTTTTGTGGGATTGGTTCTGGAGAATTGCCAACTTCCTTTTGCAAATCATGGACATGTTGTTCTCGGAGATCCTTCTCCTATTTTGTTCTATCCCATCAGTAGCACCGAGATCCGTTGTCTGGTTGATGTTCCTGGTCAGAAGGTTCCTTCTATATCAAATGGTGAAATGGAAAAGTATTTGAAGACCATAGTAGCTCCTCAGGTTCCTCCGCAAATCCATGATGCCTTTATAGCTGCTATTGACAAGGGTAATATAAGGACAATGCCAAATAGAAGCATGCCTGCTGCTCCCCAACCGACGCCCGGTGCCTTACTAATGGGTGATGCATTCAACATGCGCCACCCCCTCACCGGTGGAGGAATGACAGTTGCTTTGTCTGATATTGTTGTGCTCAGGAACCTTCTCAAGCCACTGAAGGACTTGAATGATGCACCAACTCTCTGCAAGTATCTAGAATCCTTTTACACTCTGCGAAAGCCAGTGGCTTCTACAATCAACACATTGGCAGGGGCATTATACAAGGTCTTTTGTGCATCGCCTGATCAAGCCAGGAAGGAAATGAGACAAGCTTGCTTTGATTATTTGAGCCTTGGAGGAATATTCTCAAATGGACCCGTGTCCTTGCTCTCGGGATTGAATCCTCGCCCATTAAGTCTGGTTCTCCATTTCTTTGCAGTGGCAATATACGGAGTTGGTCGCTTACTGCTTCCATTTCCTTCTCCGAAAGGCATCTGGATTGGAGCAAGATTGGTCTATAGCGCATCAGGAATCATATTTCCGATTATCAAGGCGGAAGGAGTTAGACAGATGTTCTTCCCTGCTACTGTTCCTGCTTATTACAGAAGCCCACCAGTTCTTAAAACCATAGCATGA

Protein sequence

MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Homology
BLAST of Cp4.1LG11g01020 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.1e-235
Identity = 396/525 (75.43%), Postives = 456/525 (86.86%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGAD-STPRSEGVISSSATNGECRSVDG 60
           ++D    GWI A + G  + L+F    +R +    +  T  S G  +         ++ G
Sbjct: 23  LIDQYFLGWIFAFLFGFLLLLNF--KRKREKNNSTEFGTDDSNGYYTP-------ENIAG 82

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
             DVIIVGAGVAGSALA+TL  DGR VHVIERDLTE DRIVGELLQPGGYLKLIELGL+D
Sbjct: 83  STDVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLIELGLED 142

Query: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180
           CV EIDAQ+V+GYAL+ DGKNT+LSYPLEKF SDV+GRSFHNGRF+QRMREKAASLPNVR
Sbjct: 143 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAASLPNVR 202

Query: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240
           +EQGTVTSL+E+KG++KGVQYK+K+G+E +A+APLTIVCDGCFSNLRRSLC P V+VPSC
Sbjct: 203 MEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPKVEVPSC 262

Query: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQKVP ISNGE+  YL
Sbjct: 263 FVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNGELANYL 322

Query: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVP Q++++FIAA+DKGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLTGGG
Sbjct: 323 KTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRHPLTGGG 382

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIV++R+LL+PL+DL+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCASP
Sbjct: 383 MTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 442

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480
           D+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL LHFFAVAIYGVGRLL+PFPS
Sbjct: 443 DKARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIPFPS 502

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV 525
           PK +W+GARL+  ASGIIFPIIK+EG+RQMFFPA VPAYYR+PP+
Sbjct: 503 PKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPI 538

BLAST of Cp4.1LG11g01020 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 814.7 bits (2103), Expect = 6.1e-235
Identity = 404/528 (76.52%), Postives = 453/528 (85.80%), Query Frame = 0

Query: 10  ILAAVLGLAIALSFFVSPRRNRR----GGADSTPRSEG---VISSSATNGECRSVD---G 69
           + AA L     L  F   RR RR    GGA S     G   + SSS  NG C   D    
Sbjct: 16  MFAATLLFGFVLYLFTLRRRRRRREKKGGAGSMEIINGAYKMTSSSEVNGHCTPEDIAGS 75

Query: 70  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 129
             DVIIVGAGVAGSALA+TL KDGR VHVIERDLTE DRIVGELLQPGGYLKL+ELGL+D
Sbjct: 76  SDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLVELGLED 135

Query: 130 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 189
           CV EIDAQ+V+GYAL+ DGKNT+LSYPLEKF +DV+GRSFHNGRFIQRMREKAASLPNVR
Sbjct: 136 CVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAASLPNVR 195

Query: 190 LEQGTVTSLLEEKGTIKGVQYKSKNGEE-KTAYAPLTIVCDGCFSNLRRSLCKPMVDVPS 249
           +EQGTVTSL+E+KGT+KGV+YK+KNG+E   AYAPLTIVCDGCFSNLR SLC P VDVPS
Sbjct: 196 MEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNPKVDVPS 255

Query: 250 CFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKY 309
           CFVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQKVPSI+NGE+  Y
Sbjct: 256 CFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIANGELAHY 315

Query: 310 LKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGG 369
           LKT VAPQ+PP+++ +FIAAIDKG I+TMPNRSMPA P  TPGALL+GDAFNMRHPLTGG
Sbjct: 316 LKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMRHPLTGG 375

Query: 370 GMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 429
           GMTVALSDIV++R+LL+PL+DL+D+ TLCKYLESFYTLRKPVASTINTLAGALYKVFCAS
Sbjct: 376 GMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 435

Query: 430 PDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFP 489
           PD+AR+EMR ACFDYLSLGGI S GP++LLSGLNPRP+SL  HFFAVAIYGVGRLL+PFP
Sbjct: 436 PDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGRLLIPFP 495

Query: 490 SPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLK 527
           SP+ +W+GARL+  ASGIIFPIIK+EGVRQMFFPATVPAYYR+PP+ K
Sbjct: 496 SPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPITK 543

BLAST of Cp4.1LG11g01020 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 3.2e-231
Identity = 392/526 (74.52%), Postives = 454/526 (86.31%), Query Frame = 0

Query: 7   FGWIL-----AAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD- 66
           + WIL     + ++       FFV P+RN              +S+  ++    ++ GD 
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRN------GLRHDRKTVSTVTSDVGSVNITGDT 65

Query: 67  -ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 126
            ADVI+VGAGVAGSALA+TLGKD R VHVIERDL+EPDRIVGELLQPGGYLKL+ELG++D
Sbjct: 66  VADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIED 125

Query: 127 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 186
           CVEEIDAQ+VYGYALFK+GK  +L+YPLEKF  DVSGRSFHNGRFIQRMREKAASLPNV+
Sbjct: 126 CVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQ 185

Query: 187 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 246
           LEQGTV SLLEE GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P V+VPSC
Sbjct: 186 LEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSC 245

Query: 247 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 306
           FVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ YL
Sbjct: 246 FVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYL 305

Query: 307 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 366
           KT+VAPQ+P +++D+FIAA+DKGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGGG
Sbjct: 306 KTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGG 365

Query: 367 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 426
           MTVAL+DIVVLRNLL+PL+DL+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+S 
Sbjct: 366 MTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSE 425

Query: 427 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 486
           ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFPS
Sbjct: 426 NEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPS 485

Query: 487 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVL 526
           PK IW+GA+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P +
Sbjct: 486 PKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAPTV 525

BLAST of Cp4.1LG11g01020 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 1.8e-226
Identity = 381/479 (79.54%), Postives = 434/479 (90.61%), Query Frame = 0

Query: 46  SSSATNGECRSVDG-DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELL 105
           S +  NG      G D D+IIVGAGVAG+ALAHTLGK+GR VHVIERDLTEPDRIVGELL
Sbjct: 39  SVNVRNGTLTVKSGTDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELL 98

Query: 106 QPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRF 165
           QPGGYLKLIELGL+DCV++IDAQ+V GYALFKDGK+T+LSYPL++F SDV+GRSFHNGRF
Sbjct: 99  QPGGYLKLIELGLEDCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRF 158

Query: 166 IQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSN 225
           +QRMREKA+ LPNVR+EQGTVTSL+EE G IKGVQYK+K+G+E  ++APLTIVCDGCFSN
Sbjct: 159 VQRMREKASLLPNVRMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSN 218

Query: 226 LRRSLCKPMVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPG 285
           LRRSLCKP V+VPS FVGLVLENC+LPF NHGHVVLGDPSPILFYPISS+E+RCLVDVPG
Sbjct: 219 LRRSLCKPKVEVPSNFVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPG 278

Query: 286 QKVPSISNGEMEKYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALL 345
            K+PS+++GEM  +LKT+VAPQVPPQI DAFI+A++KGNIRTMPNRSMPA P  TPGALL
Sbjct: 279 SKLPSVASGEMAHHLKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALL 338

Query: 346 MGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTI 405
           +GDAFNMRHPLTGGGMTVALSDIV+LR+LL PL DL +  +L KY+ESFYTLRKPVASTI
Sbjct: 339 LGDAFNMRHPLTGGGMTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTI 398

Query: 406 NTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFA 465
           NTLAGALYKVF ASPD AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLVLHFFA
Sbjct: 399 NTLAGALYKVFLASPDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFA 458

Query: 466 VAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           VAI+GVGRLL+P PS K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 459 VAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of Cp4.1LG11g01020 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 1.9e-223
Identity = 375/467 (80.30%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 57  VDGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELG 116
           VD   DVIIVGAGVAGSALAHTLGK+GR VHVIERD +E DRIVGELLQPGGYLKLIELG
Sbjct: 119 VDSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELG 178

Query: 117 LQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLP 176
           L+DCV++IDAQ+V GY LFKDGK+T+L+YPLE F SDV+GRSFHNGRF+QRMREKA +L 
Sbjct: 179 LEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLS 238

Query: 177 NVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 236
           NVRLEQGTVTSLLEE GTIKGV+Y++K G E  ++APLTIVCDGCFSNLRRSLCKP VDV
Sbjct: 239 NVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDV 298

Query: 237 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEME 296
           PS FVGLVLENC+LPFANHGHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM 
Sbjct: 299 PSTFVGLVLENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMA 358

Query: 297 KYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLT 356
           KYLKT VAPQVP ++ +AFI A++KGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLT
Sbjct: 359 KYLKTRVAPQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLT 418

Query: 357 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFC 416
           GGGMTVAL+DIVVLR+LL+P+++LND   L KY+ESFYTLRKPVASTINTLA ALYKVF 
Sbjct: 419 GGGMTVALADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFL 478

Query: 417 ASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLP 476
           AS D+AR EMR+ACFDYLSLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LP
Sbjct: 479 ASSDEARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLP 538

Query: 477 FPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           FPS +  W+GAR++ SAS IIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 539 FPSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of Cp4.1LG11g01020 vs. NCBI nr
Match: XP_023546518.1 (squalene monooxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1058 bits (2736), Expect = 0.0
Identity = 529/529 (100.00%), Postives = 529/529 (100.00%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Sbjct: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Cp4.1LG11g01020 vs. NCBI nr
Match: AXK50227.1 (squalene epoxidase 1 [Cucurbita pepo])

HSP 1 Score: 1052 bits (2721), Expect = 0.0
Identity = 527/529 (99.62%), Postives = 527/529 (99.62%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYL SFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLGSFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSAS IIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Sbjct: 481 KGIWIGARLVYSASRIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Cp4.1LG11g01020 vs. NCBI nr
Match: KAG6598316.1 (Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029287.1 hypothetical protein SDJN02_07625 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1052 bits (2720), Expect = 0.0
Identity = 526/529 (99.43%), Postives = 527/529 (99.62%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGV SSS TNGECRSVDGD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDGD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTA+APLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Sbjct: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Cp4.1LG11g01020 vs. NCBI nr
Match: XP_022996831.1 (squalene monooxygenase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1049 bits (2713), Expect = 0.0
Identity = 524/529 (99.05%), Postives = 526/529 (99.43%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGV SSS TNGECRSVDGD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDGD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV K+IA
Sbjct: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA 529

BLAST of Cp4.1LG11g01020 vs. NCBI nr
Match: XP_022962235.1 (squalene monooxygenase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1048 bits (2711), Expect = 0.0
Identity = 525/529 (99.24%), Postives = 526/529 (99.43%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGV SSS TNGECRSVD D
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDCD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTA+APLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Sbjct: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Cp4.1LG11g01020 vs. ExPASy TrEMBL
Match: A0A345Z0U8 (Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1052 bits (2721), Expect = 0.0
Identity = 527/529 (99.62%), Postives = 527/529 (99.62%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYL SFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLGSFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSAS IIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Sbjct: 481 KGIWIGARLVYSASRIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Cp4.1LG11g01020 vs. ExPASy TrEMBL
Match: A0A6J1K5U9 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1)

HSP 1 Score: 1049 bits (2713), Expect = 0.0
Identity = 524/529 (99.05%), Postives = 526/529 (99.43%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGV SSS TNGECRSVDGD
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDGD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPV K+IA
Sbjct: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSIA 529

BLAST of Cp4.1LG11g01020 vs. ExPASy TrEMBL
Match: A0A6J1HEG7 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1)

HSP 1 Score: 1048 bits (2711), Expect = 0.0
Identity = 525/529 (99.24%), Postives = 526/529 (99.43%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD 60
           MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGV SSS TNGECRSVD D
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVRSSSTTNGECRSVDCD 60

Query: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120
           ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC
Sbjct: 61  ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQDC 120

Query: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180
           VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL
Sbjct: 121 VEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVRL 180

Query: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240
           EQGTVTSLLEEKGTIKGVQYKSKNGEEKTA+APLTIVCDGCFSNLRRSLCKPMVDVPSCF
Sbjct: 181 EQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVPSCF 240

Query: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300
           VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK
Sbjct: 241 VGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYLK 300

Query: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360
           TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM
Sbjct: 301 TIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGGM 360

Query: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420
           TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 361 TVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 420

Query: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480
           QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP
Sbjct: 421 QARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPSP 480

Query: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529
           KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA
Sbjct: 481 KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTIA 529

BLAST of Cp4.1LG11g01020 vs. ExPASy TrEMBL
Match: A0A0A0LKI1 (Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1)

HSP 1 Score: 985 bits (2546), Expect = 0.0
Identity = 490/528 (92.80%), Postives = 506/528 (95.83%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRRG-GADSTPRSEGVISSSATNGECRSVDG 60
           MVD+C FGWI +A L   IA SFF+SPR+NRRG G +STPR + + SS+ TNGECRSVDG
Sbjct: 1   MVDHCTFGWIFSAFLAFVIAFSFFLSPRKNRRGRGTNSTPRRDCLSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180
           CVEEIDAQKVYGYALFKDGK+T+LSYPLE FQSDVSGRSFHNGRFIQRMREKAA LPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKSTRLSYPLENFQSDVSGRSFHNGRFIQRMREKAAFLPNVR 180

Query: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240
           LEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPSC
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCNPMVDVPSC 240

Query: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCAS 
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASS 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKT 527
           PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPV  +
Sbjct: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVFNS 528

BLAST of Cp4.1LG11g01020 vs. ExPASy TrEMBL
Match: A0A5A7V0X5 (Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G001090 PE=3 SV=1)

HSP 1 Score: 984 bits (2544), Expect = 0.0
Identity = 493/528 (93.37%), Postives = 506/528 (95.83%), Query Frame = 0

Query: 1   MVDYCAFGWILAAVLGLAIALSFFVSPRRNRR-GGADSTPRSEGVISSSATNGECRSVDG 60
           MVD+CAFGWI +A+L   IALS F+SP RNRR  G DSTPRS  V SS+ TNGECRSVDG
Sbjct: 1   MVDHCAFGWIFSALLAFPIALSLFLSPWRNRRVRGTDSTPRSASVSSSATTNGECRSVDG 60

Query: 61  DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120
           DADV+IVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD
Sbjct: 61  DADVVIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 120

Query: 121 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 180
           CVEEIDAQKVYGYALFKDGKNT+LSYPLE F SDVSGRSFHNGRFIQRMREKAASLPNVR
Sbjct: 121 CVEEIDAQKVYGYALFKDGKNTRLSYPLENFHSDVSGRSFHNGRFIQRMREKAASLPNVR 180

Query: 181 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 240
           LEQGTVTSLLEEKGTI GVQYKSKNGE+KTAYAPLTIVCDGCFSNLRRSLC PMVDVPS 
Sbjct: 181 LEQGTVTSLLEEKGTITGVQYKSKNGEQKTAYAPLTIVCDGCFSNLRRSLCTPMVDVPSF 240

Query: 241 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300
           FVGLVLENCQLP+AN GHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL
Sbjct: 241 FVGLVLENCQLPYANLGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 300

Query: 301 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360
           KT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480
           DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS
Sbjct: 421 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 480

Query: 481 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKT 527
            KGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYR+PPVL +
Sbjct: 481 LKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRTPPVLNS 528

BLAST of Cp4.1LG11g01020 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 802.4 bits (2071), Expect = 2.2e-232
Identity = 392/526 (74.52%), Postives = 454/526 (86.31%), Query Frame = 0

Query: 7   FGWIL-----AAVLGLAIALSFFVSPRRNRRGGADSTPRSEGVISSSATNGECRSVDGD- 66
           + WIL     + ++       FFV P+RN              +S+  ++    ++ GD 
Sbjct: 6   WNWILPLLISSLLISFVAFYGFFVKPKRN------GLRHDRKTVSTVTSDVGSVNITGDT 65

Query: 67  -ADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGLQD 126
            ADVI+VGAGVAGSALA+TLGKD R VHVIERDL+EPDRIVGELLQPGGYLKL+ELG++D
Sbjct: 66  VADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIED 125

Query: 127 CVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPNVR 186
           CVEEIDAQ+VYGYALFK+GK  +L+YPLEKF  DVSGRSFHNGRFIQRMREKAASLPNV+
Sbjct: 126 CVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQ 185

Query: 187 LEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 246
           LEQGTV SLLEE GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P V+VPSC
Sbjct: 186 LEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSC 245

Query: 247 FVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEKYL 306
           FVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI+NGEM+ YL
Sbjct: 246 FVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYL 305

Query: 307 KTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTGGG 366
           KT+VAPQ+P +++D+FIAA+DKGNI++MPNRSMPA+P PTPGALLMGDAFNMRHPLTGGG
Sbjct: 306 KTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGG 365

Query: 367 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCASP 426
           MTVAL+DIVVLRNLL+PL+DL+D  +LCKYLESFYTLRKPVA+TINTLA ALY+VFC+S 
Sbjct: 366 MTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSE 425

Query: 427 DQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPFPS 486
           ++AR EMR+ACFDYL LGG+ ++GPVSLLSGLNPRPL+LV HFFAVA+YGV RLL+PFPS
Sbjct: 426 NEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPS 485

Query: 487 PKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVL 526
           PK IW+GA+L+  ASGIIFPIIKAEGVRQMFFPATVPAYY   P +
Sbjct: 486 PKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAPTV 525

BLAST of Cp4.1LG11g01020 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 786.6 bits (2030), Expect = 1.3e-227
Identity = 381/479 (79.54%), Postives = 434/479 (90.61%), Query Frame = 0

Query: 46  SSSATNGECRSVDG-DADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELL 105
           S +  NG      G D D+IIVGAGVAG+ALAHTLGK+GR VHVIERDLTEPDRIVGELL
Sbjct: 39  SVNVRNGTLTVKSGTDVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELL 98

Query: 106 QPGGYLKLIELGLQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRF 165
           QPGGYLKLIELGL+DCV++IDAQ+V GYALFKDGK+T+LSYPL++F SDV+GRSFHNGRF
Sbjct: 99  QPGGYLKLIELGLEDCVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRF 158

Query: 166 IQRMREKAASLPNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSN 225
           +QRMREKA+ LPNVR+EQGTVTSL+EE G IKGVQYK+K+G+E  ++APLTIVCDGCFSN
Sbjct: 159 VQRMREKASLLPNVRMEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSN 218

Query: 226 LRRSLCKPMVDVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPG 285
           LRRSLCKP V+VPS FVGLVLENC+LPF NHGHVVLGDPSPILFYPISS+E+RCLVDVPG
Sbjct: 219 LRRSLCKPKVEVPSNFVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPG 278

Query: 286 QKVPSISNGEMEKYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALL 345
            K+PS+++GEM  +LKT+VAPQVPPQI DAFI+A++KGNIRTMPNRSMPA P  TPGALL
Sbjct: 279 SKLPSVASGEMAHHLKTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALL 338

Query: 346 MGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTI 405
           +GDAFNMRHPLTGGGMTVALSDIV+LR+LL PL DL +  +L KY+ESFYTLRKPVASTI
Sbjct: 339 LGDAFNMRHPLTGGGMTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTI 398

Query: 406 NTLAGALYKVFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFA 465
           NTLAGALYKVF ASPD AR EMR+ACFDYLSLGG+ S+GPV+LLSGLNPRP+SLVLHFFA
Sbjct: 399 NTLAGALYKVFLASPDDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFA 458

Query: 466 VAIYGVGRLLLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           VAI+GVGRLL+P PS K +W+GARL+ SASGIIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 459 VAIFGVGRLLVPLPSVKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of Cp4.1LG11g01020 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 776.5 bits (2004), Expect = 1.3e-224
Identity = 375/467 (80.30%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 57  VDGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELG 116
           VD   DVIIVGAGVAGSALAHTLGK+GR VHVIERD +E DRIVGELLQPGGYLKLIELG
Sbjct: 119 VDSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELG 178

Query: 117 LQDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLP 176
           L+DCV++IDAQ+V GY LFKDGK+T+L+YPLE F SDV+GRSFHNGRF+QRMREKA +L 
Sbjct: 179 LEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLS 238

Query: 177 NVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 236
           NVRLEQGTVTSLLEE GTIKGV+Y++K G E  ++APLTIVCDGCFSNLRRSLCKP VDV
Sbjct: 239 NVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDV 298

Query: 237 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEME 296
           PS FVGLVLENC+LPFANHGHVVLGDPSPIL YPISS+E+RCLVDVPGQK+P I+NGEM 
Sbjct: 299 PSTFVGLVLENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMA 358

Query: 297 KYLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLT 356
           KYLKT VAPQVP ++ +AFI A++KGNIRTMPNRSMPA P PTPGALL+GDAFNMRHPLT
Sbjct: 359 KYLKTRVAPQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLT 418

Query: 357 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFC 416
           GGGMTVAL+DIVVLR+LL+P+++LND   L KY+ESFYTLRKPVASTINTLA ALYKVF 
Sbjct: 419 GGGMTVALADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFL 478

Query: 417 ASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLP 476
           AS D+AR EMR+ACFDYLSLGG+FS+GPV+LLSGLNPRPLSLVLHFFAVAIY V RL+LP
Sbjct: 479 ASSDEARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLP 538

Query: 477 FPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPP 524
           FPS +  W+GAR++ SAS IIFPIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 539 FPSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of Cp4.1LG11g01020 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 505.8 bits (1301), Expect = 4.3e-143
Identity = 250/467 (53.53%), Postives = 328/467 (70.24%), Query Frame = 0

Query: 58  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 117
           DG  DVIIVGAGV GSALA+ L KDGR VHVIERDL EP+RI+GE +QPGG L L +LGL
Sbjct: 43  DGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSKLGL 102

Query: 118 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLE--KFQSDVSGRSFHNGRFIQRMREKAASL 177
           +DC+E IDAQK  G  ++KDGK    S+P++   F  D S RSFHNGRF+QR+R+KA+SL
Sbjct: 103 EDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKASSL 162

Query: 178 PNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVD 237
           PNVRLE+GTV SL+EEKG IKGV YK+  GEE TA APLT+VCDGC+SNLRRSL     +
Sbjct: 163 PNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDNNAE 222

Query: 238 VPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEM 297
           V S  VG + +NCQL       +++  PS  + Y ISST++RC+ +V    +PSISNGEM
Sbjct: 223 VLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISNGEM 282

Query: 298 EKYLKTIVAPQVPPQIHDAFIAAIDKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRHP 357
             ++K  +APQVP ++   F+  ID+G +I+ MP + M A      G +L+GDAFNMRHP
Sbjct: 283 ATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNMRHP 342

Query: 358 LTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKV 417
               GM V LSDI++LR LL+PL +L +A  + + ++SFY +RKP+++T+NTL  A  +V
Sbjct: 343 AIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQV 402

Query: 418 FCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLL 477
             AS D+A++ MRQ C+DYLS GG  ++G ++LL G+NPRP+SL+ H  A+ +  +G LL
Sbjct: 403 LVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHLL 462

Query: 478 LPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRS 522
            PFPSP  IW   RL   A  ++ P +KAEGV QM FP    AY +S
Sbjct: 463 SPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

BLAST of Cp4.1LG11g01020 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 495.0 bits (1273), Expect = 7.7e-140
Identity = 244/468 (52.14%), Postives = 328/468 (70.09%), Query Frame = 0

Query: 58  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 117
           DG ADVIIVGAGV GSALA+ L KDGR VHVIERD+ EP+R++GE +QPGG L L +LGL
Sbjct: 43  DGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSKLGL 102

Query: 118 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLE--KFQSDVSGRSFHNGRFIQRMREKAASL 177
           QDC+E+IDAQK  G A++KDGK     +P++   F  + S RSFHNGRF+Q++R KA SL
Sbjct: 103 QDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKAFSL 162

Query: 178 PNVRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKP-MV 237
            NVRLE+GTV SLLEEKG +KGV YK+K GEE TA APLT+VCDGC+SNLRRSL      
Sbjct: 163 SNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDDNNA 222

Query: 238 DVPSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGE 297
           ++ S  VG + +NC+L      H++L  PS  + Y ISST++RC  +V  +  PSI+NGE
Sbjct: 223 EIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIANGE 282

Query: 298 MEKYLKTIVAPQVPPQIHDAFIAAIDKG-NIRTMPNRSMPAAPQPTPGALLMGDAFNMRH 357
           M  ++K  + PQVPP++   F+  ID+G +I+ +P + M +      G +++GDAFNMRH
Sbjct: 283 MSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRH 342

Query: 358 PLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYK 417
           P+   GM V LSDI++LR LL+PL +L DA  + + + SFY +RKP+++T+NTL  A  +
Sbjct: 343 PVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQ 402

Query: 418 VFCASPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRL 477
           V   S D+A++ MRQ  +DYL  GG  ++G ++LL G+NPRPLSLV H  A+ +  +G+L
Sbjct: 403 VLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQL 462

Query: 478 LLPFPSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRS 522
           L PFPSP  IW   +L   A  ++ P +KAEGV QM FPA   AY++S
Sbjct: 463 LSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O486512.1e-23575.43Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1[more]
B7TWW56.1e-23576.52Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2[more]
Q9SM023.2e-23174.52Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1[more]
Q8VYH21.8e-22679.54Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1[more]
O810001.9e-22380.30Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... [more]
Match NameE-valueIdentityDescription
XP_023546518.10.0100.00squalene monooxygenase-like isoform X1 [Cucurbita pepo subsp. pepo][more]
AXK50227.10.099.62squalene epoxidase 1 [Cucurbita pepo][more]
KAG6598316.10.099.43Squalene monooxygenase SE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG... [more]
XP_022996831.10.099.05squalene monooxygenase-like isoform X1 [Cucurbita maxima][more]
XP_022962235.10.099.24squalene monooxygenase-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A345Z0U80.099.62Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1K5U90.099.05Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1[more]
A0A6J1HEG70.099.24Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1[more]
A0A0A0LKI10.092.80Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_2G353480 PE=3 SV=1[more]
A0A5A7V0X50.093.37Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold5... [more]
Match NameE-valueIdentityDescription
AT1G58440.12.2e-23274.52FAD/NAD(P)-binding oxidoreductase family protein [more]
AT4G37760.11.3e-22779.54squalene epoxidase 3 [more]
AT2G22830.11.3e-22480.30squalene epoxidase 2 [more]
AT5G24150.14.3e-14353.53FAD/NAD(P)-binding oxidoreductase family protein [more]
AT5G24160.17.7e-14052.14squalene monoxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00420RNGMNOXGNASEcoord: 62..84
score: 43.04
coord: 354..370
score: 32.55
coord: 339..354
score: 30.83
coord: 211..226
score: 31.25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 30..49
NoneNo IPR availablePANTHERPTHR10835:SF17SQUALENE MONOOXYGENASE-RELATEDcoord: 1..524
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 50..415
e-value: 2.3E-33
score: 117.6
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 61..436
IPR013698Squalene epoxidasePFAMPF08491SEcoord: 212..484
e-value: 1.8E-120
score: 401.1
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 1..524

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g01020.1Cp4.1LG11g01020.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0004506 squalene monooxygenase activity