Cp4.1LG11g00680 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g00680
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
LocationCp4.1LG11: 170017 .. 171777 (+)
RNA-Seq ExpressionCp4.1LG11g00680
SyntenyCp4.1LG11g00680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAACATTCAAAATGGCCTTCGATTCTCTTCCTCCTTCCCTCTCTTCTTCTTCCTCCTATGTTCTCCCCGACCATATGAGACCTGATCTCCAACACGTATCCCACGCCATCTCCCTACCCACCATTGACTTAGATCTTCACGATCAATCCCTTCTCGTCCACCAAGTATCGGAAGCCTGCCAAAGCTTCGGCTTCTTCCAAGTCATCAACCACGGGATCCCACAACGCTTATGCGACGCCGTCTTGGACACCACCACCCAATTCTTCCACCTCCCTCCCGAACAAAGAAACCAATACGTGACCACGGATCACACTAAGAAAGTTAAAATCTTCAATTACTACGCCAAGGACGTGGAGGGGCGGAAGATCAATCTGTGGAGCGAAACCTTCACGCTTCCGTGGGATCCGGCGGGGCAGGAATTTCTAAATCACGTACCCCAAAACCCACCTCAGTACAGGGAAGTGTACGGGGAGTACTCCAGGGAAATGGGAAGGTTAATGGAAAGGGTTTTGGGGCTCATGTCGTTGGGGCTGGGGCTTGACGAGGGGGCTCTCAAGAGAAGCATAGGGGAGAAGCCGGAGCTGTACTCGCAGGCTAATTACTATCCCCCCTGCCCACAACCAGAGCTCACCATGGGCCTCGGCACTCATACTGATATTGTGGCCATTGCTGTGCTGTTGCAGTCCCAAGAGGCCGCTGGCCTTCAGATCATGAAAGATCAGCAATGGGTCTCTCTCCTTCCTCATCCAAATTCATTGGTAGTTAACGTGGCCGATCAAATTCAGGTTTGTGTTTTTATTTTTCTTGCTTTCCAAGTAACCCCGGTTAGATGAACAGGACTTGTTAGGAATCACAACTCTTCTAAGGCTTCAAACCAATGCTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGTTCTAGTGACTTTGATTTGGGCTTCCCCAAAAGACTTCACACATAATATTCTTTGATTATAAATCCATGATCATTTCCTAAATTAGTAGATGTGAGACTTTCATCCAACACCTCCCCTTGAACAGAGTACGTCTCCCCTTAATCGACTCCTTTTTCTTTTGGAGTCTAAGTCATTTTTTACTATGCCTTCGAGGCGGGAGGCTCGACTCATTTTCTTTTGGAGTCTTAGTTATTTTTTTACTATGCCTTCAAGACTTACAATACTTGGTTCTCGAAAATTCTATTGACATGACTAAGTTTTTTAGGGAATAATCAAATAATACTATCTTCATTTGAATGAGACATTTTGGTAAGTAGAAAGCAAAGTCATGAGAACTTTTGCTGAAAGTGGACAATATCATACCATTGTGGAGAGTCGTGTTAATCTAACATTGTTCGAGGCGTCACAATATGATCTTCTACACTTGTGTTTCTTCCCAAATTTAGTTGTTAATTTGCTTACAAAATATTGAAAGCTTAAATCTGTCCTATATTCCATAGTTTGGAATTTAAAAAATATGAGAGTGGATTTTGAAGGTATTGAGCAATGGAAGGTATAAAAGCGTGATCCACAGGGCTGTAACCAACCGTGCGAGGAGAAGATTATCGCTAGTAATGTTCTATGCACCCAACGACGAGACGGTGATCAAGCCCATTGAGGAGCTGACGGACGAACACCACCCGCCCCTCTACAAAACCTTCACTTACAAACAATTCATGCACGAATTCCGCGCACAAGAAGGAACAATAAGAAGAGTCAAGGAGACTTTCCAGCTCACCAACTCTCTCAACACCTAA

mRNA sequence

ATGGCAACATTCAAAATGGCCTTCGATTCTCTTCCTCCTTCCCTCTCTTCTTCTTCCTCCTATGTTCTCCCCGACCATATGAGACCTGATCTCCAACACGTATCCCACGCCATCTCCCTACCCACCATTGACTTAGATCTTCACGATCAATCCCTTCTCGTCCACCAAGTATCGGAAGCCTGCCAAAGCTTCGGCTTCTTCCAAGTCATCAACCACGGGATCCCACAACGCTTATGCGACGCCGTCTTGGACACCACCACCCAATTCTTCCACCTCCCTCCCGAACAAAGAAACCAATACGTGACCACGGATCACACTAAGAAAGTTAAAATCTTCAATTACTACGCCAAGGACGTGGAGGGGCGGAAGATCAATCTGTGGAGCGAAACCTTCACGCTTCCGTGGGATCCGGCGGGGCAGGAATTTCTAAATCACGTACCCCAAAACCCACCTCAGTACAGGGAAGTGTACGGGGAGTACTCCAGGGAAATGGGAAGGTTAATGGAAAGGGTTTTGGGGCTCATGTCGTTGGGGCTGGGGCTTGACGAGGGGGCTCTCAAGAGAAGCATAGGGGAGAAGCCGGAGCTGTACTCGCAGGCTAATTACTATCCCCCCTGCCCACAACCAGAGCTCACCATGGGCCTCGGCACTCATACTGATATTGTGGCCATTGCTGTGCTGTTGCAGTCCCAAGAGGCCGCTGGCCTTCAGATCATGAAAGATCAGCAATGGGTCTCTCTCCTTCCTCATCCAAATTCATTGGTAGTTAACGTGGCCGATCAAATTCAGGTATTGAGCAATGGAAGGTATAAAAGCGTGATCCACAGGGCTGTAACCAACCGTGCGAGGAGAAGATTATCGCTAGTAATGTTCTATGCACCCAACGACGAGACGGTGATCAAGCCCATTGAGGAGCTGACGGACGAACACCACCCGCCCCTCTACAAAACCTTCACTTACAAACAATTCATGCACGAATTCCGCGCACAAGAAGGAACAATAAGAAGAGTCAAGGAGACTTTCCAGCTCACCAACTCTCTCAACACCTAA

Coding sequence (CDS)

ATGGCAACATTCAAAATGGCCTTCGATTCTCTTCCTCCTTCCCTCTCTTCTTCTTCCTCCTATGTTCTCCCCGACCATATGAGACCTGATCTCCAACACGTATCCCACGCCATCTCCCTACCCACCATTGACTTAGATCTTCACGATCAATCCCTTCTCGTCCACCAAGTATCGGAAGCCTGCCAAAGCTTCGGCTTCTTCCAAGTCATCAACCACGGGATCCCACAACGCTTATGCGACGCCGTCTTGGACACCACCACCCAATTCTTCCACCTCCCTCCCGAACAAAGAAACCAATACGTGACCACGGATCACACTAAGAAAGTTAAAATCTTCAATTACTACGCCAAGGACGTGGAGGGGCGGAAGATCAATCTGTGGAGCGAAACCTTCACGCTTCCGTGGGATCCGGCGGGGCAGGAATTTCTAAATCACGTACCCCAAAACCCACCTCAGTACAGGGAAGTGTACGGGGAGTACTCCAGGGAAATGGGAAGGTTAATGGAAAGGGTTTTGGGGCTCATGTCGTTGGGGCTGGGGCTTGACGAGGGGGCTCTCAAGAGAAGCATAGGGGAGAAGCCGGAGCTGTACTCGCAGGCTAATTACTATCCCCCCTGCCCACAACCAGAGCTCACCATGGGCCTCGGCACTCATACTGATATTGTGGCCATTGCTGTGCTGTTGCAGTCCCAAGAGGCCGCTGGCCTTCAGATCATGAAAGATCAGCAATGGGTCTCTCTCCTTCCTCATCCAAATTCATTGGTAGTTAACGTGGCCGATCAAATTCAGGTATTGAGCAATGGAAGGTATAAAAGCGTGATCCACAGGGCTGTAACCAACCGTGCGAGGAGAAGATTATCGCTAGTAATGTTCTATGCACCCAACGACGAGACGGTGATCAAGCCCATTGAGGAGCTGACGGACGAACACCACCCGCCCCTCTACAAAACCTTCACTTACAAACAATTCATGCACGAATTCCGCGCACAAGAAGGAACAATAAGAAGAGTCAAGGAGACTTTCCAGCTCACCAACTCTCTCAACACCTAA

Protein sequence

MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEACQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT
Homology
BLAST of Cp4.1LG11g00680 vs. ExPASy Swiss-Prot
Match: Q9FLV0 (Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 2.9e-63
Identity = 130/332 (39.16%), Postives = 197/332 (59.34%), Query Frame = 0

Query: 20  SYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEACQSFGFFQVINHGIPQRLC 79
           +YV P   RP L  VS     P IDL   D+S L+ Q+ +AC  FGFFQVINHG+ +++ 
Sbjct: 18  NYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVNKQII 77

Query: 80  DAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKINLWSETFTLPWDPAG 139
           D ++    +FF +  E++ +  + D TK  ++   +  +V+  ++N W +   L   P  
Sbjct: 78  DEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF--NVKKEEVNNWRDYLRLHCYPI- 137

Query: 140 QEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSIGEKPELYSQ 199
            +++N  P NPP ++E+  +YSRE+  +  ++  L+S  LGL++  +K+ +GE+ + +  
Sbjct: 138 HKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQ-HMA 197

Query: 200 ANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPHPNSLVVNVA 259
            NYYPPCP+PELT GL  HTD  A+ +LLQ     GLQI+ D QW ++ PHP++ V+N+ 
Sbjct: 198 VNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIG 257

Query: 260 DQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI---KPIEELTDEHHPPLYK 319
           DQ+Q LSNG YKSV HRAVTN    RLS+  F  P D  V+   KP+ E  D+   P+YK
Sbjct: 258 DQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYK 317

Query: 320 TFTYKQFMHEFRAQEGTIRRVKETFQLTNSLN 349
            FTY ++  +F +     R + +   L N LN
Sbjct: 318 DFTYAEYYKKFWS-----RNLDQEHCLENFLN 340

BLAST of Cp4.1LG11g00680 vs. ExPASy Swiss-Prot
Match: Q8W2X5 (Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.5e-59
Identity = 114/307 (37.13%), Postives = 183/307 (59.61%), Query Frame = 0

Query: 21  YVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEACQSFGFFQVINHGIPQRLCD 80
           YV P+  RP L  V     +P +DL   D++ +V  V +AC++ GFFQV+NHGI   L  
Sbjct: 24  YVRPESQRPRLDLVVSDARIPVVDLASPDRAAVVSAVGDACRTHGFFQVVNHGIDAALIA 83

Query: 81  AVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKINLWSETFTLPWDPAGQ 140
           +V++   +FF LP E++ +  + D  KK+++   +  +V    ++ W +   L   P  Q
Sbjct: 84  SVMEVGREFFRLPAEEKAKLYSDDPAKKIRLSTSF--NVRKETVHNWRDYLRLHCYPLHQ 143

Query: 141 EFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSIGEKPELYSQA 200
            F+   P NPP ++E+ G Y  E+  L  R+   +S  LGL+ G ++ ++GE+ E +   
Sbjct: 144 -FVPDWPSNPPSFKEIIGTYCTEVRELGFRLYEAISESLGLEGGYMRETLGEQ-EQHMAV 203

Query: 201 NYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPHPNSLVVNVAD 260
           NYYP CP+PELT GL  HTD  A+ +LL   + AGLQ++ D +W+++ P P +LV+N+ D
Sbjct: 204 NYYPQCPEPELTYGLPAHTDPNALTILLMDDQVAGLQVLNDGKWIAVNPQPGALVINIGD 263

Query: 261 QIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEELTDEHHPPLYKTFTY 320
           Q+Q LSNG+Y+SV HRAV N  R R+S+  F  P +   + P ++L  +  P +Y+ +TY
Sbjct: 264 QLQALSNGKYRSVWHRAVVNSDRERMSVASFLCPCNSVELGPAKKLITDDSPAVYRNYTY 323

Query: 321 KQFMHEF 328
            ++  +F
Sbjct: 324 DEYYKKF 326

BLAST of Cp4.1LG11g00680 vs. ExPASy Swiss-Prot
Match: Q9ZSA8 (Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 1.5e-56
Identity = 124/331 (37.46%), Postives = 193/331 (58.31%), Query Frame = 0

Query: 2   ATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHV-SHAISLPTIDL-DLH--DQSLLVHQV 61
           A  K+       S+   S+YV P   RP+L  V S   S+P IDL DLH  +++++V Q+
Sbjct: 5   AISKLLVSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQL 64

Query: 62  SEACQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAK 121
           + AC ++GFFQ+ NHG+P    + +     +FFH P  +R ++ + D TK  ++   +  
Sbjct: 65  ASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-- 124

Query: 122 DVEGRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSL 181
           +V   K+  W +   L   P  ++F+   P +P  +REV  EY+  +  L+ R+L  +S 
Sbjct: 125 NVGADKVLNWRDFLRLHCFPI-EDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISE 184

Query: 182 GLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQ 241
            LGL+   +   +G+  + +   NYYPPCP+PELT GL  H D   I VLLQ Q  +GLQ
Sbjct: 185 SLGLESDHISNILGKHAQ-HMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQ-VSGLQ 244

Query: 242 IMKDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDE 301
           + KD +WV++ P PN+ +VN+ DQ+QV+SN +YKSV+HRAV N    RLS+  FY P+ +
Sbjct: 245 VFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTD 304

Query: 302 TVIKPIEELTDEHHP-PLYKTFTYKQFMHEF 328
            VI P  EL +E     +Y+T+ + ++  +F
Sbjct: 305 AVIGPAHELVNEQDSLAIYRTYPFVEYWDKF 330

BLAST of Cp4.1LG11g00680 vs. ExPASy Swiss-Prot
Match: Q9ZWQ9 (Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.7e-55
Identity = 118/320 (36.88%), Postives = 188/320 (58.75%), Query Frame = 0

Query: 14  SLSSSSSYVLPDHMRPDLQHVSH------AISLPTIDLDLHDQSLLVHQVSEACQSFGFF 73
           SLS S+  +  + +RP+ +  +       A  +PTIDLD   Q  LV  ++EA + +G F
Sbjct: 11  SLSHSNGTIPAEFIRPEKEQPASTTYHGPAPEIPTIDLDDPVQDRLVRSIAEASREWGIF 70

Query: 74  QVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIF-NYYAKDVEGRKINL 133
           QV NHGIP  L   +     +FF LP E++  Y      K V+ +     K+VEG+K + 
Sbjct: 71  QVTNHGIPSDLICKLQAVGKEFFELPQEEKEVYSRPADAKDVQGYGTKLQKEVEGKK-SW 130

Query: 134 WSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGAL 193
               F   W P+   +    P+NPP YR V  EY++ M  +++++   +SLGLG++ G L
Sbjct: 131 VDHLFHRVWPPSSINY-RFWPKNPPSYRAVNEEYAKYMREVVDKLFTYLSLGLGVEGGVL 190

Query: 194 KRSI-GEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWV 253
           K +  G+  E   + NYYPPCP+P+L +G+  HTD+ A+ VL+ + E  GLQ+ KD +W+
Sbjct: 191 KEAAGGDDIEYMLKINYYPPCPRPDLALGVVAHTDLSALTVLVPN-EVPGLQVFKDDRWI 250

Query: 254 SLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEE 313
                PN+LV+++ DQI++LSNG+YK+V+HR   N+ + R+S  +F  P  +TV+ P+ +
Sbjct: 251 DAKYIPNALVIHIGDQIEILSNGKYKAVLHRTTVNKDKTRMSWPVFLEPPADTVVGPLPQ 310

Query: 314 LTDEHHPPLYKTFTYKQFMH 326
           L D+ +PP YK   +K + +
Sbjct: 311 LVDDENPPKYKAKKFKDYSY 327

BLAST of Cp4.1LG11g00680 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 2.9e-55
Identity = 117/320 (36.56%), Postives = 177/320 (55.31%), Query Frame = 0

Query: 21  YVLPDHMRP-DLQHVSHAISLPTIDLD-------LHDQSLLVHQVSEACQSFGFFQVINH 80
           Y+ P  +RP   +    A ++P IDL+       L D  +++ ++SEAC+ +GFFQV+NH
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSD-DVIMARISEACRGWGFFQVVNH 101

Query: 81  GIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAK-DVEGRKINLWSETF 140
           G+   L DA  +   +FFH+P   +  Y  +  T +     Y ++  VE      WS+ +
Sbjct: 102 GVKPELMDAARENWREFFHMPVNAKETYSNSPRTYE----GYGSRLGVEKGASLDWSDYY 161

Query: 141 TLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSI- 200
            L   P   +  N  P  PP  REV  EY  E+ +L  R++ ++S  LGL E   + +  
Sbjct: 162 FLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFG 221

Query: 201 GEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPH 260
           GE      + NYYP CP+PEL +GL  H+D   + +LL   +  GLQ+ KD  W+++ PH
Sbjct: 222 GENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPH 281

Query: 261 PNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEELTDEH 320
           P++ +VN+ DQIQ+LSN  YKSV HR + N  + R+SL  FY P  +  I+P++EL   H
Sbjct: 282 PHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTH 341

Query: 321 HPPLYKTFTYKQFMHEFRAQ 331
           +PPLY   T+ Q+    R Q
Sbjct: 342 NPPLYPPMTFDQYRLFIRTQ 356

BLAST of Cp4.1LG11g00680 vs. NCBI nr
Match: XP_023545451.1 (protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 714 bits (1844), Expect = 5.21e-260
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0

Query: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEA 60
           MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEA
Sbjct: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEA 60

Query: 61  CQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVE 120
           CQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVE
Sbjct: 61  CQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVE 120

Query: 121 GRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLG 180
           GRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLG
Sbjct: 121 GRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLG 180

Query: 181 LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMK 240
           LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMK
Sbjct: 181 LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMK 240

Query: 241 DQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI 300
           DQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI
Sbjct: 241 DQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI 300

Query: 301 KPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT 349
           KPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT
Sbjct: 301 KPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT 349

BLAST of Cp4.1LG11g00680 vs. NCBI nr
Match: XP_022962245.1 (protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita moschata])

HSP 1 Score: 689 bits (1777), Expect = 8.53e-250
Identity = 335/349 (95.99%), Postives = 342/349 (97.99%), Query Frame = 0

Query: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEA 60
           MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSH ISLPTIDL+LHDQSLLVHQVSEA
Sbjct: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHTISLPTIDLNLHDQSLLVHQVSEA 60

Query: 61  CQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVE 120
           CQSFGFFQV NHGIPQRLCDAVLDTT QFFHLPP+QRNQYVTTDHTKKVKIFNYYAKDVE
Sbjct: 61  CQSFGFFQVTNHGIPQRLCDAVLDTTIQFFHLPPQQRNQYVTTDHTKKVKIFNYYAKDVE 120

Query: 121 GRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLG 180
           GRKINLWSETFTLPWDP GQEFL+ VPQNPPQYREVYGEY+REMGRLMERVLGLMSLGLG
Sbjct: 121 GRKINLWSETFTLPWDPVGQEFLSDVPQNPPQYREVYGEYAREMGRLMERVLGLMSLGLG 180

Query: 181 LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMK 240
           LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQ+Q+AAGLQIMK
Sbjct: 181 LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQDAAGLQIMK 240

Query: 241 DQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI 300
           DQQWVSLLP PNSLVVNVADQIQVLSNGRYKSVIHRAVTN ARRRLSLVMFYAPNDETVI
Sbjct: 241 DQQWVSLLPRPNSLVVNVADQIQVLSNGRYKSVIHRAVTNSARRRLSLVMFYAPNDETVI 300

Query: 301 KPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT 349
           KPIEELTDEHHPPLYKTFTYKQFMH+FRAQEGTIRRVKETFQLTNSLNT
Sbjct: 301 KPIEELTDEHHPPLYKTFTYKQFMHQFRAQEGTIRRVKETFQLTNSLNT 349

BLAST of Cp4.1LG11g00680 vs. NCBI nr
Match: XP_022996769.1 (protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita maxima])

HSP 1 Score: 674 bits (1740), Expect = 4.84e-244
Identity = 331/356 (92.98%), Postives = 340/356 (95.51%), Query Frame = 0

Query: 1   MATFKMAFDSLPPSLSSSSS-------YVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLL 60
           MATFKMAFDSLPPSLSSSSS       YVLPDHMRPDLQ VSH IS+PTIDLDLHD+SLL
Sbjct: 1   MATFKMAFDSLPPSLSSSSSSSSSSSSYVLPDHMRPDLQRVSHTISVPTIDLDLHDRSLL 60

Query: 61  VHQVSEACQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFN 120
           VHQVSEAC SFGFFQV NHGIPQ LCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFN
Sbjct: 61  VHQVSEACHSFGFFQVTNHGIPQHLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFN 120

Query: 121 YYAKDVEGRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLG 180
           YYAKDVEGRKINLWSETFTLPWDP GQ+FLN VPQNPPQYREVYGEY+REMGRLMERVLG
Sbjct: 121 YYAKDVEGRKINLWSETFTLPWDPMGQKFLNDVPQNPPQYREVYGEYAREMGRLMERVLG 180

Query: 181 LMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEA 240
           LMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQ+QEA
Sbjct: 181 LMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQEA 240

Query: 241 AGLQIMKDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYA 300
           AGLQIMKD QWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRAR RLSLVMFYA
Sbjct: 241 AGLQIMKDHQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARGRLSLVMFYA 300

Query: 301 PNDETVIKPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT 349
           PNDETV+KPIEELTDEHHPPLYK+FTYKQFMHEFRAQEGTIRRVKE+FQLTNSL+T
Sbjct: 301 PNDETVMKPIEELTDEHHPPLYKSFTYKQFMHEFRAQEGTIRRVKESFQLTNSLDT 356

BLAST of Cp4.1LG11g00680 vs. NCBI nr
Match: KAG6598240.1 (Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029218.1 Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 670 bits (1728), Expect = 1.86e-242
Identity = 325/341 (95.31%), Postives = 332/341 (97.36%), Query Frame = 0

Query: 6   MAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEACQSFG 65
           MAFDSLPPSLSSSSSYVLPDHMRPDLQHVSH ISLPTIDL+LHDQSLLVHQVSEACQSFG
Sbjct: 1   MAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHTISLPTIDLNLHDQSLLVHQVSEACQSFG 60

Query: 66  FFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKIN 125
           FFQVINHGIPQRLCDAVLDTT QFFHLPP+QRNQYVTTDHTKKVKIFNYYAKDVEGRKIN
Sbjct: 61  FFQVINHGIPQRLCDAVLDTTIQFFHLPPQQRNQYVTTDHTKKVKIFNYYAKDVEGRKIN 120

Query: 126 LWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGA 185
           LWSETFTLPWDP GQEF+N VPQNPPQYREVYGEY+REMGRLMERVLGLMSLGLGLDEGA
Sbjct: 121 LWSETFTLPWDPVGQEFVNDVPQNPPQYREVYGEYAREMGRLMERVLGLMSLGLGLDEGA 180

Query: 186 LKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWV 245
           LKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQ+QE AGLQIMKDQQWV
Sbjct: 181 LKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQEPAGLQIMKDQQWV 240

Query: 246 SLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEE 305
           SLLP PNSLVVNVADQIQVLSNGRYKSVIHRAVTN A  RLSLVMFYAPNDETVIKPIEE
Sbjct: 241 SLLPRPNSLVVNVADQIQVLSNGRYKSVIHRAVTNSAMTRLSLVMFYAPNDETVIKPIEE 300

Query: 306 LTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNS 346
           LTDEHHPPLYKTFTYKQFMH+FRAQEGTIRRVKETFQLTN+
Sbjct: 301 LTDEHHPPLYKTFTYKQFMHQFRAQEGTIRRVKETFQLTNT 341

BLAST of Cp4.1LG11g00680 vs. NCBI nr
Match: XP_022132267.1 (flavanone 3-dioxygenase 2-like [Momordica charantia])

HSP 1 Score: 526 bits (1355), Expect = 1.98e-185
Identity = 259/347 (74.64%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 2   ATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEAC 61
           A F+MA DS PPSLS S  +VLP+ +RP LQ VSH IS+P IDL   DQSL+V +VSEAC
Sbjct: 3   ARFQMACDSQPPSLSPS--FVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEAC 62

Query: 62  QSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEG 121
           Q +GFFQVINHGIPQ LCD VLDT  QFFHLPP+ RNQYVTTDH KKVKIFNYYA +VEG
Sbjct: 63  QQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA-NVEG 122

Query: 122 RKINLWSETFTLPW--DPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGL 181
           R++N+WSETFTLPW  DP   E+L+ VP+ P  YREVYGEY+REMG LMER+LGLMSLGL
Sbjct: 123 RRVNMWSETFTLPWNPDPVETEYLDVVPEEPAVYREVYGEYAREMGSLMERLLGLMSLGL 182

Query: 182 GLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIM 241
           GL+EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ+Q  +GL IM
Sbjct: 183 GLEEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNQAPSGLHIM 242

Query: 242 KDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETV 301
           KD  WVS+ P PNSLVVN+ADQ+QVLSNGRYKSVIHRAVTN    R+S+VMFYAPND+TV
Sbjct: 243 KDDHWVSVDPIPNSLVVNLADQVQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTV 302

Query: 302 IKPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNS 346
           IKPIEELTDEHHPPLY +FTY++FM  F AQEG  RRVKETF+L ++
Sbjct: 303 IKPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 346

BLAST of Cp4.1LG11g00680 vs. ExPASy TrEMBL
Match: A0A6J1HEH8 (protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita moschata OX=3662 GN=LOC111462753 PE=3 SV=1)

HSP 1 Score: 689 bits (1777), Expect = 4.13e-250
Identity = 335/349 (95.99%), Postives = 342/349 (97.99%), Query Frame = 0

Query: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEA 60
           MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSH ISLPTIDL+LHDQSLLVHQVSEA
Sbjct: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHTISLPTIDLNLHDQSLLVHQVSEA 60

Query: 61  CQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVE 120
           CQSFGFFQV NHGIPQRLCDAVLDTT QFFHLPP+QRNQYVTTDHTKKVKIFNYYAKDVE
Sbjct: 61  CQSFGFFQVTNHGIPQRLCDAVLDTTIQFFHLPPQQRNQYVTTDHTKKVKIFNYYAKDVE 120

Query: 121 GRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLG 180
           GRKINLWSETFTLPWDP GQEFL+ VPQNPPQYREVYGEY+REMGRLMERVLGLMSLGLG
Sbjct: 121 GRKINLWSETFTLPWDPVGQEFLSDVPQNPPQYREVYGEYAREMGRLMERVLGLMSLGLG 180

Query: 181 LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMK 240
           LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQ+Q+AAGLQIMK
Sbjct: 181 LDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQDAAGLQIMK 240

Query: 241 DQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI 300
           DQQWVSLLP PNSLVVNVADQIQVLSNGRYKSVIHRAVTN ARRRLSLVMFYAPNDETVI
Sbjct: 241 DQQWVSLLPRPNSLVVNVADQIQVLSNGRYKSVIHRAVTNSARRRLSLVMFYAPNDETVI 300

Query: 301 KPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT 349
           KPIEELTDEHHPPLYKTFTYKQFMH+FRAQEGTIRRVKETFQLTNSLNT
Sbjct: 301 KPIEELTDEHHPPLYKTFTYKQFMHQFRAQEGTIRRVKETFQLTNSLNT 349

BLAST of Cp4.1LG11g00680 vs. ExPASy TrEMBL
Match: A0A6J1K9L9 (protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491903 PE=3 SV=1)

HSP 1 Score: 674 bits (1740), Expect = 2.34e-244
Identity = 331/356 (92.98%), Postives = 340/356 (95.51%), Query Frame = 0

Query: 1   MATFKMAFDSLPPSLSSSSS-------YVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLL 60
           MATFKMAFDSLPPSLSSSSS       YVLPDHMRPDLQ VSH IS+PTIDLDLHD+SLL
Sbjct: 1   MATFKMAFDSLPPSLSSSSSSSSSSSSYVLPDHMRPDLQRVSHTISVPTIDLDLHDRSLL 60

Query: 61  VHQVSEACQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFN 120
           VHQVSEAC SFGFFQV NHGIPQ LCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFN
Sbjct: 61  VHQVSEACHSFGFFQVTNHGIPQHLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFN 120

Query: 121 YYAKDVEGRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLG 180
           YYAKDVEGRKINLWSETFTLPWDP GQ+FLN VPQNPPQYREVYGEY+REMGRLMERVLG
Sbjct: 121 YYAKDVEGRKINLWSETFTLPWDPMGQKFLNDVPQNPPQYREVYGEYAREMGRLMERVLG 180

Query: 181 LMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEA 240
           LMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQ+QEA
Sbjct: 181 LMSLGLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQTQEA 240

Query: 241 AGLQIMKDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYA 300
           AGLQIMKD QWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRAR RLSLVMFYA
Sbjct: 241 AGLQIMKDHQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARGRLSLVMFYA 300

Query: 301 PNDETVIKPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNSLNT 349
           PNDETV+KPIEELTDEHHPPLYK+FTYKQFMHEFRAQEGTIRRVKE+FQLTNSL+T
Sbjct: 301 PNDETVMKPIEELTDEHHPPLYKSFTYKQFMHEFRAQEGTIRRVKESFQLTNSLDT 356

BLAST of Cp4.1LG11g00680 vs. ExPASy TrEMBL
Match: A0A6J1BTD6 (flavanone 3-dioxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111005163 PE=3 SV=1)

HSP 1 Score: 526 bits (1355), Expect = 9.58e-186
Identity = 259/347 (74.64%), Postives = 292/347 (84.15%), Query Frame = 0

Query: 2   ATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEAC 61
           A F+MA DS PPSLS S  +VLP+ +RP LQ VSH IS+P IDL   DQSL+V +VSEAC
Sbjct: 3   ARFQMACDSQPPSLSPS--FVLPEDIRPRLQQVSHGISVPVIDLAADDQSLVVGKVSEAC 62

Query: 62  QSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEG 121
           Q +GFFQVINHGIPQ LCD VLDT  QFFHLPP+ RNQYVTTDH KKVKIFNYYA +VEG
Sbjct: 63  QQYGFFQVINHGIPQALCDRVLDTVAQFFHLPPQHRNQYVTTDHKKKVKIFNYYA-NVEG 122

Query: 122 RKINLWSETFTLPW--DPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGL 181
           R++N+WSETFTLPW  DP   E+L+ VP+ P  YREVYGEY+REMG LMER+LGLMSLGL
Sbjct: 123 RRVNMWSETFTLPWNPDPVETEYLDVVPEEPAVYREVYGEYAREMGSLMERLLGLMSLGL 182

Query: 182 GLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIM 241
           GL+EGALKR IG KPELYSQANYYPPCP PELTMGLG HTDIVAIAVLLQ+Q  +GL IM
Sbjct: 183 GLEEGALKRRIGAKPELYSQANYYPPCPDPELTMGLGAHTDIVAIAVLLQNQAPSGLHIM 242

Query: 242 KDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETV 301
           KD  WVS+ P PNSLVVN+ADQ+QVLSNGRYKSVIHRAVTN    R+S+VMFYAPND+TV
Sbjct: 243 KDDHWVSVDPIPNSLVVNLADQVQVLSNGRYKSVIHRAVTNSVMERVSMVMFYAPNDDTV 302

Query: 302 IKPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQLTNS 346
           IKPIEELTDEHHPPLY +FTY++FM  F AQEG  RRVKETF+L ++
Sbjct: 303 IKPIEELTDEHHPPLYTSFTYREFMQRFHAQEGRRRRVKETFELPHT 346

BLAST of Cp4.1LG11g00680 vs. ExPASy TrEMBL
Match: A0A7N2R1R3 (Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 409 bits (1052), Expect = 7.63e-140
Identity = 198/344 (57.56%), Postives = 257/344 (74.71%), Query Frame = 0

Query: 1   MATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEA 60
           M T    F +  P LS S +++LP+  RP L  V  + S+PTIDL+ HD   LV ++S+A
Sbjct: 1   METTPFQFTNNTP-LSLSPNFILPEENRPILSEVFSSASIPTIDLNNHDIDDLVSKISQA 60

Query: 61  CQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVE 120
           C+ +GFFQ+INHG+P+ LC  VL   T+FF LPPE+R Q++TTDH K VK+FNYY K VE
Sbjct: 61  CEKYGFFQIINHGVPKELCQRVLAVVTEFFQLPPEERAQFLTTDHRKLVKVFNYYQK-VE 120

Query: 121 GR-KINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGL 180
           G+ K+ +W ETFT PW P  + F + +P NPPQYREV+ EY+RE+G LM+R+L LMS GL
Sbjct: 121 GQGKVLMWGETFTHPWHPT-ENFTHFLPTNPPQYREVFTEYAREIGGLMDRLLSLMSQGL 180

Query: 181 GLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIM 240
           GL++  LKR +GE+P  Y+QANYYPPCP PELTMGL  H DI A+ VLLQ +  +GLQ++
Sbjct: 181 GLEKDCLKRKLGERPATYTQANYYPPCPDPELTMGLPAHNDISALVVLLQMEGVSGLQVV 240

Query: 241 KDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETV 300
            D +WV + P P++ VVN+ADQ+QVLSNGRYKSV+HRAVTN    RLSL MFYAPN++TV
Sbjct: 241 IDGKWVPVEPVPDAFVVNIADQMQVLSNGRYKSVLHRAVTNNRLARLSLPMFYAPNNDTV 300

Query: 301 IKPIEELTDEHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQL 343
           I PIE+L DE HPP+Y+ ++YK++M EF  QEG  RRVKE F+L
Sbjct: 301 IGPIEDLIDEVHPPMYRNYSYKEYMDEFYRQEGARRRVKEAFEL 341

BLAST of Cp4.1LG11g00680 vs. ExPASy TrEMBL
Match: A0A7N2LA75 (Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 397 bits (1020), Expect = 7.02e-135
Identity = 191/335 (57.01%), Postives = 250/335 (74.63%), Query Frame = 0

Query: 15  LSSSSSYVLPDHMRPDLQHVSHAISLPTIDLDLHD------QSLLVHQVSEACQSFGFFQ 74
           LS S  ++LP+  RP L  V  + S+P IDL+ H+       S LV ++S+AC+ +GFFQ
Sbjct: 15  LSLSPDFILPEFKRPILSKVFPSDSIPIIDLNDHEIDEGQGSSPLVSKISQACEEYGFFQ 74

Query: 75  VINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKINLWS 134
           +INHG+P+ LC  VL   T+FF LP E+R QY+T DHTK+VK+FNYY K     K+ +WS
Sbjct: 75  IINHGVPKELCQKVLAVVTEFFQLPHEERAQYLTKDHTKQVKVFNYYQKYEGLGKVIMWS 134

Query: 135 ETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKR 194
           ETF+ PW P  ++F + +P NPPQYREV+ EY++E+G LM+R+L L+S GLGL++  LK+
Sbjct: 135 ETFSHPWHPI-EDFTHLLPTNPPQYREVFAEYAKEIGALMDRLLSLISQGLGLEKDCLKK 194

Query: 195 SIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLL 254
            IGE+  LYSQANYYPPCP PELTMG+  H DIVA+ VLLQS+   GLQ++K+++WV + 
Sbjct: 195 KIGERASLYSQANYYPPCPDPELTMGIPAHNDIVALTVLLQSEGVTGLQVIKEEKWVPVD 254

Query: 255 PHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEELTD 314
           P P+S VVN+ADQIQVLSNGRYKSV HRAVTN+   R+SL MFYAPN++ VI PIE+L D
Sbjct: 255 PVPHSFVVNLADQIQVLSNGRYKSVDHRAVTNKWLPRVSLAMFYAPNNDIVIGPIEDLID 314

Query: 315 EHHPPLYKTFTYKQFMHEFRAQEGTIRRVKETFQL 343
           E HPP Y+ ++YK+FM EF  QEG  RRVKE F+L
Sbjct: 315 EEHPPKYRNYSYKEFMDEFYRQEGKRRRVKEAFEL 348

BLAST of Cp4.1LG11g00680 vs. TAIR 10
Match: AT5G24530.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.0e-64
Identity = 130/332 (39.16%), Postives = 197/332 (59.34%), Query Frame = 0

Query: 20  SYVLPDHMRPDLQHVSHAISLPTIDLDLHDQSLLVHQVSEACQSFGFFQVINHGIPQRLC 79
           +YV P   RP L  VS     P IDL   D+S L+ Q+ +AC  FGFFQVINHG+ +++ 
Sbjct: 18  NYVRPISDRPRLSEVSQLEDFPLIDLSSTDRSFLIQQIHQACARFGFFQVINHGVNKQII 77

Query: 80  DAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKINLWSETFTLPWDPAG 139
           D ++    +FF +  E++ +  + D TK  ++   +  +V+  ++N W +   L   P  
Sbjct: 78  DEMVSVAREFFSMSMEEKMKLYSDDPTKTTRLSTSF--NVKKEEVNNWRDYLRLHCYPI- 137

Query: 140 QEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSIGEKPELYSQ 199
            +++N  P NPP ++E+  +YSRE+  +  ++  L+S  LGL++  +K+ +GE+ + +  
Sbjct: 138 HKYVNEWPSNPPSFKEIVSKYSREVREVGFKIEELISESLGLEKDYMKKVLGEQGQ-HMA 197

Query: 200 ANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPHPNSLVVNVA 259
            NYYPPCP+PELT GL  HTD  A+ +LLQ     GLQI+ D QW ++ PHP++ V+N+ 
Sbjct: 198 VNYYPPCPEPELTYGLPAHTDPNALTILLQDTTVCGLQILIDGQWFAVNPHPDAFVINIG 257

Query: 260 DQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVI---KPIEELTDEHHPPLYK 319
           DQ+Q LSNG YKSV HRAVTN    RLS+  F  P D  V+   KP+ E  D+   P+YK
Sbjct: 258 DQLQALSNGVYKSVWHRAVTNTENPRLSVASFLCPADCAVMSPAKPLWEAEDDETKPVYK 317

Query: 320 TFTYKQFMHEFRAQEGTIRRVKETFQLTNSLN 349
            FTY ++  +F +     R + +   L N LN
Sbjct: 318 DFTYAEYYKKFWS-----RNLDQEHCLENFLN 340

BLAST of Cp4.1LG11g00680 vs. TAIR 10
Match: AT4G10500.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 221.5 bits (563), Expect = 1.1e-57
Identity = 124/331 (37.46%), Postives = 193/331 (58.31%), Query Frame = 0

Query: 2   ATFKMAFDSLPPSLSSSSSYVLPDHMRPDLQHV-SHAISLPTIDL-DLH--DQSLLVHQV 61
           A  K+       S+   S+YV P   RP+L  V S   S+P IDL DLH  +++++V Q+
Sbjct: 5   AISKLLVSDFASSVHIPSNYVRPISDRPNLSEVESSGDSIPLIDLRDLHGPNRAVIVQQL 64

Query: 62  SEACQSFGFFQVINHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAK 121
           + AC ++GFFQ+ NHG+P    + +     +FFH P  +R ++ + D TK  ++   +  
Sbjct: 65  ASACSTYGFFQIKNHGVPDTTVNKMQTVAREFFHQPESERVKHYSADPTKTTRLSTSF-- 124

Query: 122 DVEGRKINLWSETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSL 181
           +V   K+  W +   L   P  ++F+   P +P  +REV  EY+  +  L+ R+L  +S 
Sbjct: 125 NVGADKVLNWRDFLRLHCFPI-EDFIEEWPSSPISFREVTAEYATSVRALVLRLLEAISE 184

Query: 182 GLGLDEGALKRSIGEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQ 241
            LGL+   +   +G+  + +   NYYPPCP+PELT GL  H D   I VLLQ Q  +GLQ
Sbjct: 185 SLGLESDHISNILGKHAQ-HMAFNYYPPCPEPELTYGLPGHKDPTVITVLLQDQ-VSGLQ 244

Query: 242 IMKDQQWVSLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDE 301
           + KD +WV++ P PN+ +VN+ DQ+QV+SN +YKSV+HRAV N    RLS+  FY P+ +
Sbjct: 245 VFKDDKWVAVSPIPNTFIVNIGDQMQVISNDKYKSVLHRAVVNTENERLSIPTFYFPSTD 304

Query: 302 TVIKPIEELTDEHHP-PLYKTFTYKQFMHEF 328
            VI P  EL +E     +Y+T+ + ++  +F
Sbjct: 305 AVIGPAHELVNEQDSLAIYRTYPFVEYWDKF 330

BLAST of Cp4.1LG11g00680 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 217.2 bits (552), Expect = 2.0e-56
Identity = 117/320 (36.56%), Postives = 177/320 (55.31%), Query Frame = 0

Query: 21  YVLPDHMRP-DLQHVSHAISLPTIDLD-------LHDQSLLVHQVSEACQSFGFFQVINH 80
           Y+ P  +RP   +    A ++P IDL+       L D  +++ ++SEAC+ +GFFQV+NH
Sbjct: 42  YIKPASLRPTTTEDAPTATNIPIIDLEGLFSEEGLSD-DVIMARISEACRGWGFFQVVNH 101

Query: 81  GIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAK-DVEGRKINLWSETF 140
           G+   L DA  +   +FFH+P   +  Y  +  T +     Y ++  VE      WS+ +
Sbjct: 102 GVKPELMDAARENWREFFHMPVNAKETYSNSPRTYE----GYGSRLGVEKGASLDWSDYY 161

Query: 141 TLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSI- 200
            L   P   +  N  P  PP  REV  EY  E+ +L  R++ ++S  LGL E   + +  
Sbjct: 162 FLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGRIMRVLSTNLGLKEDKFQEAFG 221

Query: 201 GEKPELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPH 260
           GE      + NYYP CP+PEL +GL  H+D   + +LL   +  GLQ+ KD  W+++ PH
Sbjct: 222 GENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILLPDDQVFGLQVRKDDTWITVKPH 281

Query: 261 PNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEELTDEH 320
           P++ +VN+ DQIQ+LSN  YKSV HR + N  + R+SL  FY P  +  I+P++EL   H
Sbjct: 282 PHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSLAFFYNPKSDIPIQPLQELVSTH 341

Query: 321 HPPLYKTFTYKQFMHEFRAQ 331
           +PPLY   T+ Q+    R Q
Sbjct: 342 NPPLYPPMTFDQYRLFIRTQ 356

BLAST of Cp4.1LG11g00680 vs. TAIR 10
Match: AT4G10490.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 214.5 bits (545), Expect = 1.3e-55
Identity = 118/315 (37.46%), Postives = 187/315 (59.37%), Query Frame = 0

Query: 19  SSYVLPDHMRPDLQHV-SHAISLPTIDL-DLH--DQSLLVHQVSEACQSFGFFQVINHGI 78
           S+YV P   RP +  V +   S+P IDL DLH  +++ +++Q + AC S GFFQ+ NHG+
Sbjct: 20  SNYVRPVSDRPKMSEVQTSGDSIPLIDLHDLHGPNRADIINQFAHACSSCGFFQIKNHGV 79

Query: 79  PQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAKDVEGRKINLWSETFTLP 138
           P+     +++   +FF     +R ++ + D  K  ++   +  +V   K++ W +   L 
Sbjct: 80  PEETIKKMMNAAREFFRQSESERVKHYSADTKKTTRLSTSF--NVSKEKVSNWRDFLRLH 139

Query: 139 WDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKRSIGEKP 198
             P  ++F+N  P  P  +REV  EY+  +  L+  +L  +S  LGL +  +  +IG+  
Sbjct: 140 CYPI-EDFINEWPSTPISFREVTAEYATSVRALVLTLLEAISESLGLAKDRVSNTIGKHG 199

Query: 199 ELYSQANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIMKDQQWVSLLPHPNSL 258
           + +   NYYP CPQPELT GL  H D   I VLLQ  E +GLQ+ KD +W+++ P PN+ 
Sbjct: 200 Q-HMAINYYPRCPQPELTYGLPGHKDANLITVLLQ-DEVSGLQVFKDGKWIAVNPVPNTF 259

Query: 259 VVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEELTDEHH--P 318
           +VN+ DQ+QV+SN +YKSV+HRAV N    R+S+  FY P+++ VI P +EL +E    P
Sbjct: 260 IVNLGDQMQVISNEKYKSVLHRAVVNSDMERISIPTFYCPSEDAVISPAQELINEEEDSP 319

Query: 319 PLYKTFTYKQFMHEF 328
            +Y+ FTY ++  +F
Sbjct: 320 AIYRNFTYAEYFEKF 329

BLAST of Cp4.1LG11g00680 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 207.2 bits (526), Expect = 2.1e-53
Identity = 118/325 (36.31%), Postives = 175/325 (53.85%), Query Frame = 0

Query: 21  YVLPDHMRPDL----QHVSHAISLPTIDL------DLHDQSLLVHQVSEACQSFGFFQVI 80
           YV P   RP++    +H     ++P IDL      DL  Q+  + ++S+AC+  GFFQV+
Sbjct: 29  YVKPLSQRPNITPHNKHNPQTTTIPIIDLGRLYTDDLTLQAKTLDEISKACRELGFFQVV 88

Query: 81  NHGIPQRLCDAVLDTTTQFFHLPPEQRNQYVTTDHTKKVKIFNYYAK--DVEGRKINLWS 140
           NHG+  +L D    T  +FF+LP E +N      H    K +  Y     VE   I  WS
Sbjct: 89  NHGMSPQLMDQAKATWREFFNLPMELKNM-----HANSPKTYEGYGSRLGVEKGAILDWS 148

Query: 141 ETFTLPWDPAGQEFLNHVPQNPPQYREVYGEYSREMGRLMERVLGLMSLGLGLDEGALKR 200
           + + L + P+  +     P  P   RE+  +Y +EM +L E ++ ++S  LGL E  L+ 
Sbjct: 149 DYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMVKLCENLMKILSKNLGLQEDRLQN 208

Query: 201 SIGEKPELYS--QANYYPPCPQPELTMGLGTHTDIVAIAVLLQSQEAAGLQIM-KDQQWV 260
           + G K E     + NYYP CPQPELT+G+  H+D   + +LL  ++ A LQ+   D  W+
Sbjct: 209 AFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGGLTILLPDEQVASLQVRGSDDAWI 268

Query: 261 SLLPHPNSLVVNVADQIQVLSNGRYKSVIHRAVTNRARRRLSLVMFYAPNDETVIKPIEE 320
           ++ P P++ +VN+ DQIQ+LSN  YKSV HR + N    RLSL  FY P     I+P++E
Sbjct: 269 TVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPENERLSLAFFYNPKGNVPIEPLKE 328

Query: 321 LTDEHHPPLYKTFTYKQFMHEFRAQ 331
           L     P LY + TY ++    R Q
Sbjct: 329 LVTVDSPALYSSTTYDRYRQFIRTQ 348

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FLV02.9e-6339.16Protein DOWNY MILDEW RESISTANCE 6 OS=Arabidopsis thaliana OX=3702 GN=DMR6 PE=1 S... [more]
Q8W2X51.5e-5937.13Flavanone 3-dioxygenase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=F3H-2 PE=1... [more]
Q9ZSA81.5e-5637.46Protein DMR6-LIKE OXYGENASE 1 OS=Arabidopsis thaliana OX=3702 GN=DLO1 PE=1 SV=1[more]
Q9ZWQ91.7e-5536.88Flavonol synthase/flavanone 3-hydroxylase OS=Citrus unshiu OX=55188 GN=FLS PE=1 ... [more]
Q9FFF62.9e-5536.56Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_023545451.15.21e-260100.00protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita pepo subsp. pepo][more]
XP_022962245.18.53e-25095.99protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita moschata][more]
XP_022996769.14.84e-24492.98protein DOWNY MILDEW RESISTANCE 6-like [Cucurbita maxima][more]
KAG6598240.11.86e-24295.31Protein DOWNY MILDEW RESISTANCE 6, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022132267.11.98e-18574.64flavanone 3-dioxygenase 2-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1HEH84.13e-25095.99protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A6J1K9L92.34e-24492.98protein DOWNY MILDEW RESISTANCE 6-like OS=Cucurbita maxima OX=3661 GN=LOC1114919... [more]
A0A6J1BTD69.58e-18674.64flavanone 3-dioxygenase 2-like OS=Momordica charantia OX=3673 GN=LOC111005163 PE... [more]
A0A7N2R1R37.63e-14057.56Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
A0A7N2LA757.02e-13557.01Fe2OG dioxygenase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G24530.12.0e-6439.162-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10500.11.1e-5737.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT5G05600.12.0e-5636.562-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT4G10490.11.3e-5537.462-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT3G55970.12.1e-5336.31jasmonate-regulated gene 21 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 13..332
e-value: 6.9E-91
score: 307.0
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 200..294
e-value: 2.7E-24
score: 85.6
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 40..141
e-value: 7.8E-20
score: 71.8
NoneNo IPR availablePANTHERPTHR47990:SF1241-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASEcoord: 10..343
NoneNo IPR availablePANTHERPTHR479902-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATEDcoord: 10..343
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 19..328
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 193..294
score: 11.43433

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g00680.1Cp4.1LG11g00680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding