Cp4.1LG11g00140 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG11g00140
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionpiRNA biogenesis protein EXD1-like isoform X2
LocationCp4.1LG11: 475412 .. 481192 (+)
RNA-Seq ExpressionCp4.1LG11g00140
SyntenyCp4.1LG11g00140
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGAGGGAAAATTGAAAGATTCGCTCAAATGGCGAACACTCCTCCTTCTCACCAGACGTATATTCCTCTGCCTTCGGACTCAGGTCACTCTCTCTTTCCCCTTTCTCGCGTTCTTGGATTGAACTTCCATTTTCATTCATGATCAATTTCGTATTGTTTTCTTTGTCTATCTTTCTTCCTTTTAATGAATGCTTCGTTATTGCAAGTTCGTGGTCTATGGATTGACGTGCGATTCTTCGTAGCAAGTTGTTTTCTCTCTTATCGAAGCTTTAATTGCTGTTTGTTTGGCCAATCAGAAATCGTGGAATATACTTGACTGATGTTGGAAACTGAATTCTGATGCAAGAGAATCACAATGATGTAGTTATCAATGGTTAGAGTAGAAGAATTTGTTTGGAAACTTCAAAAGACATGTTGCTCTAAGTATAGGCCTGTGTTTTCTCTATTTCCATCGTTCTTTCGCCTTTTTACGTCACTTGGATTTAGTTCCAGTTGCTGCGGATAGGTTTGGTTTTCTTGATTGCTTTACCCGCGCATGTTCCATCAAGTTTTTTAGTTAGCATCTTCATTTTTGCTTCGAGACGATGGAATCTTTCTTGTGGTGGATAGTTTGCGCAATGTTTGATATAAAATATAACTGGAGATGTTAATTCTCCCCTCGTTCTCCAGCTCCTGTCCTCCATACGATCATTTCGCTCCAAGATTTTTATCATCTTCCAATGTTTTGGTTTTCTTGATTGCTTTACCCGCGCATGCTCCATCAAGTTTTTTAGTTAGTATGTTTGTTTTTGCTTCGAGACGATGGAATATTTTCTGTGGTTGATACTTTGCGCACTGTTTGATATAAAATAGAACTGGAGAGGTTAATTCTCCCATCGTTCCCCAGCTCCTGTCCTTCATACGATCATTTCGCTCCAAGATTTTTATCATCTTCCAATGAGTTCCATTTCATGTGGATTTCAGGTGAAAATCAGAACGATCCTGAAGCCAACAAAACCTTGGTTCCTATTCATATTGTTACTCATGCATCTCAACTCCCTAACGAATTTGTTGATCCATCACCTGAAAAGCCTCTGGTAGTTGGCTTTGATTGTGAAGGTGTCAGCCTGTGTCGCCATGGAAGTCTTTGTATCATGCAGGTAAATAAAATGCTAAGAACAAATCGCAGTTTTACCATCCTTGTGGAGAGTGAAATTGTATGGCAGAGGCGGATGTGCTAGCTAACCTTCCCAACTGAGGGAGTCAATGGTTCTAACTTATAATTTCATTCTTTTAGTCATCTGGTGATACTGTCAATTCATAGTCCAAGCTGCTTTCTTGCAAGAAAACAACTAATATTATAGCTTAAGAAATTTAATTTGCGCTCCTGGATATTCCTCGACTTGTAGCTATAGTAACTCATCCTTTAAATTACAGGGTTGATTACTGTCAAAGGCTTGGACATTCAAGAATGTTCAGTTTCTACTTTACTAAAAAACAGTGATTCAAATTAGTTGACATTATCTATAAATCTGTGTTGTTCATTTCTTGGCAGATTGCATTTCCAGATGCTATATATCTGGTTGATGCTGTTCAGGGAGGAGAGGAACTTGTGAAAGTCTGTAAGCCTGCCCTTGAGTCCACATATGTCACGAAAGTTATTCATGATTGTAAACGAGATAGTGAGGTCTCGTCTGAACTTTGTTTCCATTACAGATTGCCGTCACAGAGACATGACATGTTTTAGCTAACGTTGACTCTATTTTTTTCCCTATAGGCATTGTACTTTCAGTTTGGTATTAAGTTGAACAACGTTATTGATACACAGGTGATTATCAATTGTGAGAAGCCTGACACTCTTTACTTGTTTACACCCTTTTCTCATTCATTATATTTTATCTTATTTTAGAGTGCTTAGCCACTTAGTGTGCCAATTCCATGTTACTAGAACATAAGAATATTTTTTGTGCTTTTCTTCTTCTATGTTTTCTTGGATATAAAATGAAACTATCATATCAGGAAGATGGATTCTACTATTCCACTGATCGTTGTTCCATTATTTCTTATCTAAGTTCCTAGAAGTTTTCATGATGTTAATTTTTCACATCTACTTTGCACTTGTACGATTTGAATCTACAGTCTGCCAGTATTTTTATAAATTTATAATTCTACGTACTCATTTTAAAGGTTTATGTAAATTTTCTCTCAATGAACTCTAATCTTAACTTACTATGGATTATTAACTGATTGAAATCTGCTATCTGATAATTTTTAAGGTTGAAAGTCTGCATGTCTTCAAAAAGGAAAAAGAGAGAGAGAGAGAGAGAGAGAGTGGTTATCTTGTGACACAAATGCCAATAAAGAGTATGATTTTCTTGATTTAAAAGAATGATCTGAACAATTCATTTTAAGTTTGAGCTACCTTGTAATTACTTTGGAGCCAAGTGTTTGTATGTGTAAGCTGCAATATGGAGGAAGGCTGAGTTATTTGGACCTTCCTGCTTTAATTTTCAATTGTATTACTTGATTCAGATTGCATATTCACTTATAGAGGAGCAAGAGGGATGGACAAAGACGCCAGATAACTATATCTCCTTTGTTGGTCTTCTTGCAGATTCACGTTATTGTGGTATTATACTAATCCTGGTATCTAGATATTCTCCATTCTTTATCATGGAATTTCATGCATTTTTCTTTTTCTTCTAAATATCTCGGATAAAATTACCTATACTCACTCAAAAATATGAACTGATATTTCTTGCCATCTTCAAAAGCAGCAAAGCTTGATTTATTGCTTGCCATCTTCATGTTAAGAAGCTCCATACTTCCTACTTATTTTGGGGGTCATGTTTCCATGAGCTGCATGGTTAATTAACCAGAACCTCCTGCCTGAAAAGGAAAAAAAAAAAAGTATAAATTTAAATTTGGAAGCCATCACATGACTAATCCCTAGCGATACGGTGGAGGGACAATAATTGCACTTGGTTAATTGGATCTGTGTCTCTAAGCGAAATTAATTGAGCTTGTGGGTGCTATAATAACATCAACATAAAGAACTTGATTTTAAATGGAAAGGGACCTTCAGAACACTTCTCGCCCCTTTCCATCTCAAAATATCGAGCTCTTAATTTCATAGACCAGGTACAAGCTTGAGAATTGTGATAAGATTATATTTGGAAACGTTATGTGGATTGCGCATTCATCTCTCGCATACTTTGTTTCTCATTCTTCATTTTGTGTAATGTTACAATTCTGGTACCAAGGAAATCAGAATGACCGACTAGGAGCCTAATTTTAGAGTCTAAAGGCATCGTACATTGATTGTGTACACTTAATTGGCACTTGACTCAGTCTTTGTAAGCATGAGGAGGTTAAGTAAAGTATAACCAAAGGGTTCAGTGCTCATGAGGCTTTGTAAGAGAATTGCATTTCATGCATTCTCCATTCAAAACTGTTGCTATTCGTGCTTGTATATTTTTCATTCCTCTTGGTTGCCGTTGTAATACCCTTTGTTTCATTTTTCTTTCTTTCCCTTGTTTGTTGCCTGGAAACGTTTCCTTCCTGGAGAAATCTACGTGNGCTTTTTTTTTTTTTTTTTTTTTCACTTTTTTCTTGTTACTGAAAATGTTGGTGGATGGATCTAAATTGTTTGGCATGCTAATTTAATTATCAGAATTTTCTGGTAGGAATAATCTAGATTCTACGTCAAGTTCTTGTGGTCAGTGGAGAAGTTGACAAGCTTTTGTTATATGAATGCATTTCTTTATTATTTTAAAACTTAATGCTTTTTTCTGCTAATGCTTACATTTTTATTCTATATTGAAATAATATGTAATCCTCGCTCATAAATGTAGGTGTATCATATGTGGAGAAGGAAGAGGTCCGCCTCCTACTTAGGCAGGTTAGTTTTTTCATGGTCGTATTGCTGTGGATATTGTTGTATCTTGGCAAGAAGTGTTCCATATATTAAGCATTGTTTTTCGTTATGAGGTATTTTATAGGTCAGATGTGCTTGATTCCTAAATGTAGTATCCTTCTTTTGGAGTAGACAAGTATAAATATAATGTAGAGACATTTCAAGTCCATTGAGTTAACAAAAAGGACATGGAAACTAAGATTATTAGTCTGCAGGTACTGTAATTATGTCCATAATATGATAAGATTCAAAATGTTATCTCGTCATAAAATTATATGCAGGTATCGTGTAATTGTTCCATAAAAACTAAATTCTAAATTTGTATTTGACAGGACCCGAAGTTTTGGACCTATAGACCATTGTCTGAATTGATGGTCCGTGCAGCTGCTGATGATGTACGCTTTCTGCTTTACATCTATCACAAGTTGATGGAGAAATTGAATCATCAATCGCTGTGGTACCTCGCAGTGCGTGGTGCTTTGTACTGCCGGTGTTTCTGCATCAGTGATAATGGATATGCTGACTGGCCTCCTCTCCCTTCTGTTCCAGGTTTTCTTTAAGAACACACCTTACCATGTGCAGTCCATATAATGAGAATAATTCCAAATTTAACATTCTAATATGCTAAGTTGATTAAACTATCATTGCAAACTTTTCAACATAATATGATTCTGTGGTGTTTCAATATTGTTGGCTTCTTTTATGTTATGATTTCACAATTGATATTGGGTTTAAAATTTAAAGTGGCTATTTAGCAGTGTGAGAAATGTTGGTTCACCATCTTTCATTCAATTATCCCAATGCTGCTGGAATCTGCAAAACATCAAAATATGTTAAAATTTATAATGTTGTATTATCTAATTATTATCGACACATATGGTATGTGGTTTCTGTCCTTAGGGTTCTTAGACTACGTTTCATTTCAAATCTTATTTTTTCATTTGATGCGTCTAAAACCTTTTGTTACATGGTCTCAATTGATCACCTTTATTGGTCTTACATGTTTTTTCAGATAACCTTGTAGTAGAGGGCAAAGCTCCTGAAGAAGAAATTCTTTCAGTCTTAGACATTCCCCGTGGAATGATGGGTCGCGTAATTGGTAGGAGAGGAGCCTCAATTTTGGCAATAAAGGAATCTTGCAAGTAATTCATTATCGACAAATCCCTTTAGACTTCATTTATATCCTTTAGGCTAAAAAAATACCATAGCCGTTGTTTTTTCTCACCTTGGGTTCTTAAAATGCAGGGCGGAAATTCTCGTTGGCGGCACCAAGGGCCCACCGGACAAGGTAAGTCTCTCTTTTCATTCACTTCAAAACAGACCACTATAAAGCCTAAAATTGAATGCTATTCTTTGAACTTTCCTGTGAATTTCCTGGATTCCTCTCTCCATTTGCTTCAAATCCCACTGCCTATATAAGACCTCAACACCTCCTTTTGAGTTATTTGTTAAAACCCCCTGGAACAAACAAAGGGGACTCGCTCTGCTTGTCTCGAAGTCTCTGAGTATCAGCCAAAATCCTTTTGAATGTTGATGGACATTGAAAGTTGTTCTGTCAGTAAGATGAATGTAATTGTGGTGATGTAGGTTTTCGTCATTGGACCCGTGAGGCAGGTAAGGAAGGCAGAAGCTATGATACGAGGAAGCTTGCTTGAAATGTGATTGAATGAATATGAAAACCGTGTTGTGATGAAAATGAACGCAAAATCCTCAATATTAAGAATACAGTTCCAGCTGCTAATACTAGTAGATCCCGTATGGAAGGGATTACTTTACATACTTTACAATCTTTTGTATAGGTACAGTAGCTTTGAGAAGCAAATTTTATGTGAAAAATATTATCAAATCAAAATATGAAAATTGAAATAAAACTTTGTTTTTACCTT

mRNA sequence

CAGAGGGAAAATTGAAAGATTCGCTCAAATGGCGAACACTCCTCCTTCTCACCAGACGTATATTCCTCTGCCTTCGGACTCAGGTGAAAATCAGAACGATCCTGAAGCCAACAAAACCTTGGTTCCTATTCATATTGTTACTCATGCATCTCAACTCCCTAACGAATTTGTTGATCCATCACCTGAAAAGCCTCTGGTAGTTGGCTTTGATTGTGAAGGTGTCAGCCTGTGTCGCCATGGAAGTCTTTGTATCATGCAGATTGCATTTCCAGATGCTATATATCTGGTTGATGCTGTTCAGGGAGGAGAGGAACTTGTGAAAGTCTGTAAGCCTGCCCTTGAGTCCACATATGTCACGAAAGTTATTCATGATTGTAAACGAGATAGTGAGGCATTGTACTTTCAGTTTGGTATTAAGTTGAACAACGTTATTGATACACAGATTGCATATTCACTTATAGAGGAGCAAGAGGGATGGACAAAGACGCCAGATAACTATATCTCCTTTGTTGGTCTTCTTGCAGATTCACGTTATTGTGGTGTATCATATGTGGAGAAGGAAGAGGTCCGCCTCCTACTTAGGCAGGACCCGAAGTTTTGGACCTATAGACCATTGTCTGAATTGATGGTCCGTGCAGCTGCTGATGATGTACGCTTTCTGCTTTACATCTATCACAAGTTGATGGAGAAATTGAATCATCAATCGCTGTGGTACCTCGCAGTGCGTGGTGCTTTGTACTGCCGGTGTTTCTGCATCAGTGATAATGGATATGCTGACTGGCCTCCTCTCCCTTCTGTTCCAGATAACCTTGTAGTAGAGGGCAAAGCTCCTGAAGAAGAAATTCTTTCAGTCTTAGACATTCCCCGTGGAATGATGGGTCGCGTAATTGGTAGGAGAGGAGCCTCAATTTTGGCAATAAAGGAATCTTGCAAGGCGGAAATTCTCGTTGGCGGCACCAAGGGCCCACCGGACAAGGTTTTCGTCATTGGACCCGTGAGGCAGGTAAGGAAGGCAGAAGCTATGATACGAGGAAGCTTGCTTGAAATGTGATTGAATGAATATGAAAACCGTGTTGTGATGAAAATGAACGCAAAATCCTCAATATTAAGAATACAGTTCCAGCTGCTAATACTAGTAGATCCCGTATGGAAGGGATTACTTTACATACTTTACAATCTTTTGTATAGGTACAGTAGCTTTGAGAAGCAAATTTTATGTGAAAAATATTATCAAATCAAAATATGAAAATTGAAATAAAACTTTGTTTTTACCTT

Coding sequence (CDS)

ATGGCGAACACTCCTCCTTCTCACCAGACGTATATTCCTCTGCCTTCGGACTCAGGTGAAAATCAGAACGATCCTGAAGCCAACAAAACCTTGGTTCCTATTCATATTGTTACTCATGCATCTCAACTCCCTAACGAATTTGTTGATCCATCACCTGAAAAGCCTCTGGTAGTTGGCTTTGATTGTGAAGGTGTCAGCCTGTGTCGCCATGGAAGTCTTTGTATCATGCAGATTGCATTTCCAGATGCTATATATCTGGTTGATGCTGTTCAGGGAGGAGAGGAACTTGTGAAAGTCTGTAAGCCTGCCCTTGAGTCCACATATGTCACGAAAGTTATTCATGATTGTAAACGAGATAGTGAGGCATTGTACTTTCAGTTTGGTATTAAGTTGAACAACGTTATTGATACACAGATTGCATATTCACTTATAGAGGAGCAAGAGGGATGGACAAAGACGCCAGATAACTATATCTCCTTTGTTGGTCTTCTTGCAGATTCACGTTATTGTGGTGTATCATATGTGGAGAAGGAAGAGGTCCGCCTCCTACTTAGGCAGGACCCGAAGTTTTGGACCTATAGACCATTGTCTGAATTGATGGTCCGTGCAGCTGCTGATGATGTACGCTTTCTGCTTTACATCTATCACAAGTTGATGGAGAAATTGAATCATCAATCGCTGTGGTACCTCGCAGTGCGTGGTGCTTTGTACTGCCGGTGTTTCTGCATCAGTGATAATGGATATGCTGACTGGCCTCCTCTCCCTTCTGTTCCAGATAACCTTGTAGTAGAGGGCAAAGCTCCTGAAGAAGAAATTCTTTCAGTCTTAGACATTCCCCGTGGAATGATGGGTCGCGTAATTGGTAGGAGAGGAGCCTCAATTTTGGCAATAAAGGAATCTTGCAAGGCGGAAATTCTCGTTGGCGGCACCAAGGGCCCACCGGACAAGGTTTTCGTCATTGGACCCGTGAGGCAGGTAAGGAAGGCAGAAGCTATGATACGAGGAAGCTTGCTTGAAATGTGA

Protein sequence

MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Homology
BLAST of Cp4.1LG11g00140 vs. ExPASy Swiss-Prot
Match: Q8NHP7 (piRNA biogenesis protein EXD1 OS=Homo sapiens OX=9606 GN=EXD1 PE=1 SV=4)

HSP 1 Score: 87.8 bits (216), Expect = 2.6e-16
Identity = 53/172 (30.81%), Postives = 88/172 (51.16%), Query Frame = 0

Query: 53  EKPLVVGFDCEGVSLCRHGSLCIMQIAFPDAIYLVDA-VQGGEELVKVCKPALESTYVTK 112
           +K  V+    EG ++CRHG LC +Q+A    +YL D  + G        +  LE   + K
Sbjct: 96  KKQNVLSVAAEGANVCRHGKLCWLQVATNCRVYLFDIFLLGSRAFHNGLQMILEDKRILK 155

Query: 113 VIHDCKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCG 172
           VIHDC+  S+ L  Q+GI LNNV DTQ+A  L    E     P+   +    L       
Sbjct: 156 VIHDCRWLSDCLSHQYGILLNNVFDTQVADVLQFSMETGGYLPNCITTLQESLIKHLQVA 215

Query: 173 VSYVE-KEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 223
             Y+   E+ + L++++P+ W  RP+S  +++  A +  +LL +   L++++
Sbjct: 216 PKYLSFLEKRQKLIQENPEVWFIRPVSPSLLKILALEATYLLPLRLALLDEM 267

BLAST of Cp4.1LG11g00140 vs. ExPASy Swiss-Prot
Match: Q8CDF7 (piRNA biogenesis protein EXD1 OS=Mus musculus OX=10090 GN=Exd1 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-15
Identity = 55/173 (31.79%), Postives = 88/173 (50.87%), Query Frame = 0

Query: 53  EKPLVVGFDCEGVSLCRHGSLCIMQIAFPDAIYLVDA-VQGGEELVKVCKPALESTYVTK 112
           +K  V+    EG ++CRHG LC +Q+A    +YL D  + G        +  LE   + K
Sbjct: 153 KKQSVLSVAAEGANVCRHGKLCWLQVATNSRVYLFDIFLLGSRAFNNGLQMILEDKRILK 212

Query: 113 VIHDCKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFV--GLLADSRY 172
           VIHDC+  S+ L  Q+GI LNNV DTQ+A  L    E     P N IS +   L+   + 
Sbjct: 213 VIHDCRWLSDCLSHQYGIMLNNVFDTQVADVLQFSMETGGFLP-NCISTLQESLIRHLKV 272

Query: 173 CGVSYVEKEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 223
                   EE +  ++++P+ W  RPL   +++  A +  +LL +   L++++
Sbjct: 273 APRYLFFLEERQKRIQENPEIWLTRPLPPSLLKILALETTYLLPLRLVLLDEV 324

BLAST of Cp4.1LG11g00140 vs. ExPASy Swiss-Prot
Match: Q6NRD5 (piRNA biogenesis protein EXD1 OS=Xenopus laevis OX=8355 GN=exd1 PE=2 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.6e-10
Identity = 47/173 (27.17%), Postives = 85/173 (49.13%), Query Frame = 0

Query: 57  VVGFDCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVK-VCKPALESTYVTKVIHD 116
           V+     G ++CRHG L  +Q A    +YL D +  G ++ K   +  LE   + KVIHD
Sbjct: 122 VISIGAVGQNICRHGKLSWLQFATRSRVYLFDVLVLGSKVFKNGLQMVLEDKGILKVIHD 181

Query: 117 CKRDSEALYFQFGIKLNNVIDTQI--AYSLIEEQEGW----TKTPDNYISFVGLLADSRY 176
           C+   + L  Q+GI LNNV DTQ+   Y    E  G+    T+T +  +     +  S+ 
Sbjct: 182 CRWLGDILSHQYGIILNNVFDTQVGDVYLFSMETGGFLPHGTRTLEECLIHHLSMLPSK- 241

Query: 177 CGVSYVEKEEVRLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 223
             VS++   +   L ++    W  RP+   +++  + +V +L+ +   +++ +
Sbjct: 242 --VSFLAHRQT--LTKEYHDIWFDRPMDPTLLKLLSLEVTYLMPLRSAMLDAM 289

BLAST of Cp4.1LG11g00140 vs. ExPASy Swiss-Prot
Match: Q0P3U3 (piRNA biogenesis protein EXD1 OS=Danio rerio OX=7955 GN=exd1 PE=2 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.0e-08
Identity = 45/170 (26.47%), Postives = 76/170 (44.71%), Query Frame = 0

Query: 57  VVGFDCEGVSLCRHGSLCIMQIAFPDAIYLVD-AVQGGEELVKVCKPALESTYVTKVIHD 116
           V+G   +         LC +Q+A    +YL D  + GG          LE+T++ KV+HD
Sbjct: 136 VIGIGADVYGQSGQERLCWLQVATKKVVYLFDILLLGGPAFKNGLSMILENTHILKVLHD 195

Query: 117 CKRDSEALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYV 176
           C+  +  L  +F ++L NV DTQ+A  L+   E     PD   S   LL    +  ++  
Sbjct: 196 CRCITRCLRTEFRVQLTNVFDTQVAELLLFFNESGGFLPDRPASLPELL--QLHLRLTTA 255

Query: 177 EKEEV---RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKL 223
           E + +   +   R+  + W  RP    ++      V+ LL +   L++ L
Sbjct: 256 EIQPLCSKQQQSRECVQLWYVRPCPPDLLSLMCSSVQHLLSLRLLLLDAL 303

BLAST of Cp4.1LG11g00140 vs. ExPASy Swiss-Prot
Match: P56960 (Exosome component 10 OS=Mus musculus OX=10090 GN=Exosc10 PE=1 SV=2)

HSP 1 Score: 58.9 bits (141), Expect = 1.3e-07
Identity = 45/159 (28.30%), Postives = 71/159 (44.65%), Query Frame = 0

Query: 71  GSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIK 130
           G  C+MQI+     ++VD ++   ++  +   +L    + KV H    D E L   FG+ 
Sbjct: 324 GLTCLMQISTRTEDFIVDTLELRSDMY-ILNESLTDPAIVKVFHGADSDIEWLQKDFGLY 383

Query: 131 LNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKF 190
           + N+ DT  A  L+        + D+ +          YCGV   ++ ++          
Sbjct: 384 VVNMFDTHQAARLLNLAR---HSLDHLLRL--------YCGVESNKQYQL--------AD 443

Query: 191 WTYRPLSELMVRAAADDVRFLLYIYHK----LMEKLNHQ 226
           W  RPL E M+  A DD  +LLYIY +    L E+ NHQ
Sbjct: 444 WRIRPLPEEMLSYARDDTHYLLYIYDRMRLELWERGNHQ 462

BLAST of Cp4.1LG11g00140 vs. NCBI nr
Match: XP_023545692.1 (piRNA biogenesis protein EXD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700 bits (1806), Expect = 1.60e-254
Identity = 340/340 (100.00%), Postives = 340/340 (100.00%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340

BLAST of Cp4.1LG11g00140 vs. NCBI nr
Match: KAG6598308.1 (piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 693 bits (1788), Expect = 8.88e-252
Identity = 337/340 (99.12%), Postives = 338/340 (99.41%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIY KLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYRKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCI+DNGYADWPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCINDNGYADWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340

BLAST of Cp4.1LG11g00140 vs. NCBI nr
Match: KAG7029279.1 (piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 692 bits (1787), Expect = 1.26e-251
Identity = 336/340 (98.82%), Postives = 338/340 (99.41%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEE+
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEI 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIY KLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYRKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCI+DNGYADWPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCINDNGYADWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340

BLAST of Cp4.1LG11g00140 vs. NCBI nr
Match: XP_022997512.1 (piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 686 bits (1771), Expect = 3.34e-249
Identity = 336/340 (98.82%), Postives = 337/340 (99.12%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTP SHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTP-SHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLC HGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCCHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGG KGPPDKVFVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 301 CKAEILVGGAKGPPDKVFVIGPVRQVRKAEAMIRGSLLEI 339

BLAST of Cp4.1LG11g00140 vs. NCBI nr
Match: XP_022961700.1 (piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 682 bits (1760), Expect = 1.59e-247
Identity = 334/340 (98.24%), Postives = 336/340 (98.82%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTP S QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTP-SRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCI+DNGYA+WPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 339

BLAST of Cp4.1LG11g00140 vs. ExPASy TrEMBL
Match: A0A6J1K589 (piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111492408 PE=4 SV=1)

HSP 1 Score: 686 bits (1771), Expect = 1.62e-249
Identity = 336/340 (98.82%), Postives = 337/340 (99.12%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTP SHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTP-SHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLC HGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCCHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGG KGPPDKVFVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 301 CKAEILVGGAKGPPDKVFVIGPVRQVRKAEAMIRGSLLEI 339

BLAST of Cp4.1LG11g00140 vs. ExPASy TrEMBL
Match: A0A6J1HD17 (piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462393 PE=4 SV=1)

HSP 1 Score: 682 bits (1760), Expect = 7.69e-248
Identity = 334/340 (98.24%), Postives = 336/340 (98.82%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MANTP S QTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF
Sbjct: 1   MANTP-SRQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGVSLCRHG LCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS
Sbjct: 61  DCEGVSLCRHGRLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV
Sbjct: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC
Sbjct: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240

Query: 241 FCISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300
           FCI+DNGYA+WPPLPSVPDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES
Sbjct: 241 FCINDNGYANWPPLPSVPDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKES 300

Query: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 340
           CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 301 CKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLEM 339

BLAST of Cp4.1LG11g00140 vs. ExPASy TrEMBL
Match: A0A6J1KE47 (piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111492408 PE=4 SV=1)

HSP 1 Score: 656 bits (1692), Expect = 1.43e-237
Identity = 319/323 (98.76%), Postives = 321/323 (99.38%), Query Frame = 0

Query: 18  SGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGSLCIMQ 77
           +GENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLC HGSLCIMQ
Sbjct: 11  NGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCCHGSLCIMQ 70

Query: 78  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 137
           IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT
Sbjct: 71  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 130

Query: 138 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 197
           QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS
Sbjct: 131 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 190

Query: 198 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV 257
           ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV
Sbjct: 191 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV 250

Query: 258 PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 317
           PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGG KGPPDKV
Sbjct: 251 PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGAKGPPDKV 310

Query: 318 FVIGPVRQVRKAEAMIRGSLLEM 340
           FVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 311 FVIGPVRQVRKAEAMIRGSLLEI 333

BLAST of Cp4.1LG11g00140 vs. ExPASy TrEMBL
Match: A0A6J1HB37 (piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111462393 PE=4 SV=1)

HSP 1 Score: 655 bits (1689), Expect = 4.09e-237
Identity = 318/323 (98.45%), Postives = 321/323 (99.38%), Query Frame = 0

Query: 18  SGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGSLCIMQ 77
           +GENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHG LCIMQ
Sbjct: 11  NGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGRLCIMQ 70

Query: 78  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 137
           IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT
Sbjct: 71  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 130

Query: 138 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 197
           QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS
Sbjct: 131 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 190

Query: 198 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV 257
           ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCI+DNGYA+WPPLPSV
Sbjct: 191 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCINDNGYANWPPLPSV 250

Query: 258 PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 317
           PDNLVVE KAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV
Sbjct: 251 PDNLVVEDKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 310

Query: 318 FVIGPVRQVRKAEAMIRGSLLEM 340
           FVIGPVRQVRKAEAMIRGSLLEM
Sbjct: 311 FVIGPVRQVRKAEAMIRGSLLEM 333

BLAST of Cp4.1LG11g00140 vs. ExPASy TrEMBL
Match: A0A6J1K7P2 (uncharacterized protein LOC111492408 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111492408 PE=4 SV=1)

HSP 1 Score: 654 bits (1686), Expect = 1.64e-236
Identity = 318/323 (98.45%), Postives = 320/323 (99.07%), Query Frame = 0

Query: 18  SGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCRHGSLCIMQ 77
           + ENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLC HGSLCIMQ
Sbjct: 20  ASENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGFDCEGVSLCCHGSLCIMQ 79

Query: 78  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 137
           IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT
Sbjct: 80  IAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIKLNNVIDT 139

Query: 138 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 197
           QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS
Sbjct: 140 QIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKFWTYRPLS 199

Query: 198 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV 257
           ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV
Sbjct: 200 ELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRCFCISDNGYADWPPLPSV 259

Query: 258 PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGTKGPPDKV 317
           PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGG KGPPDKV
Sbjct: 260 PDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKESCKAEILVGGAKGPPDKV 319

Query: 318 FVIGPVRQVRKAEAMIRGSLLEM 340
           FVIGPVRQVRKAEAMIRGSLLE+
Sbjct: 320 FVIGPVRQVRKAEAMIRGSLLEI 342

BLAST of Cp4.1LG11g00140 vs. TAIR 10
Match: AT2G25910.1 (3'-5' exonuclease domain-containing protein / K homology domain-containing protein / KH domain-containing protein )

HSP 1 Score: 513.1 bits (1320), Expect = 1.7e-145
Identity = 238/340 (70.00%), Postives = 289/340 (85.00%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MA++ P+   ++P+P + G      EAN+  VPI+IVT   QLP +F++PSPEK LV+GF
Sbjct: 1   MASSSPTQLAHVPIPPEPGGRSPTQEANEPPVPIYIVTDPFQLPADFLNPSPEKKLVIGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGV LCRHG LCIMQIAF +AIYLVD ++GGE ++K CKPALES Y+TKVIHDCKRDS
Sbjct: 61  DCEGVDLCRHGKLCIMQIAFSNAIYLVDVIEGGEVIMKACKPALESNYITKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGI+L+NV+DTQIAYSLIEEQEG  +  D+YISFV LLAD RYCG+SY EKEEV
Sbjct: 121 EALYFQFGIRLHNVVDTQIAYSLIEEQEGRRRPLDDYISFVSLLADPRYCGISYEEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           R+L+RQDPKFWTYRP++ELM+RAAADDVRFLLY+YHK+M KLN +SLW+LAVRGALYCRC
Sbjct: 181 RVLMRQDPKFWTYRPMTELMIRAAADDVRFLLYLYHKMMGKLNQRSLWHLAVRGALYCRC 240

Query: 241 F-CISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKE 300
             C++D  +ADWP +P +PDNL  E +  EEEILSVLD+P G MGRVIGR+GASILAIKE
Sbjct: 241 LCCMNDADFADWPTVPPIPDNLKSEDQCLEEEILSVLDVPPGKMGRVIGRKGASILAIKE 300

Query: 301 SCKAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLE 340
           +C AEIL+GG KGPPDK+FVIGPVR+VRKAEA++RG +++
Sbjct: 301 ACNAEILIGGAKGPPDKIFVIGPVREVRKAEAILRGRMID 340

BLAST of Cp4.1LG11g00140 vs. TAIR 10
Match: AT2G25910.2 (3'-5' exonuclease domain-containing protein / K homology domain-containing protein / KH domain-containing protein )

HSP 1 Score: 508.4 bits (1308), Expect = 4.3e-144
Identity = 238/341 (69.79%), Postives = 289/341 (84.75%), Query Frame = 0

Query: 1   MANTPPSHQTYIPLPSDSGENQNDPEANKTLVPIHIVTHASQLPNEFVDPSPEKPLVVGF 60
           MA++ P+   ++P+P + G      EAN+  VPI+IVT   QLP +F++PSPEK LV+GF
Sbjct: 1   MASSSPTQLAHVPIPPEPGGRSPTQEANEPPVPIYIVTDPFQLPADFLNPSPEKKLVIGF 60

Query: 61  DCEGVSLCRHGSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDS 120
           DCEGV LCRHG LCIMQIAF +AIYLVD ++GGE ++K CKPALES Y+TKVIHDCKRDS
Sbjct: 61  DCEGVDLCRHGKLCIMQIAFSNAIYLVDVIEGGEVIMKACKPALESNYITKVIHDCKRDS 120

Query: 121 EALYFQFGIKLNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEV 180
           EALYFQFGI+L+NV+DTQIAYSLIEEQEG  +  D+YISFV LLAD RYCG+SY EKEEV
Sbjct: 121 EALYFQFGIRLHNVVDTQIAYSLIEEQEGRRRPLDDYISFVSLLADPRYCGISYEEKEEV 180

Query: 181 RLLLRQDPKFWTYRPLSELMVRAAADDVRFLLYIYHKLMEKLNHQSLWYLAVRGALYCRC 240
           R+L+RQDPKFWTYRP++ELM+RAAADDVRFLLY+YHK+M KLN +SLW+LAVRGALYCRC
Sbjct: 181 RVLMRQDPKFWTYRPMTELMIRAAADDVRFLLYLYHKMMGKLNQRSLWHLAVRGALYCRC 240

Query: 241 F-CISDNGYADWPPLPSVPDNLVVEGKAPEEEILSVLDIPRGMMGRVIGRRGASILAIKE 300
             C++D  +ADWP +P +PDNL  E +  EEEILSVLD+P G MGRVIGR+GASILAIKE
Sbjct: 241 LCCMNDADFADWPTVPPIPDNLKSEDQCLEEEILSVLDVPPGKMGRVIGRKGASILAIKE 300

Query: 301 SC-KAEILVGGTKGPPDKVFVIGPVRQVRKAEAMIRGSLLE 340
           +C  AEIL+GG KGPPDK+FVIGPVR+VRKAEA++RG +++
Sbjct: 301 ACNSAEILIGGAKGPPDKIFVIGPVREVRKAEAILRGRMID 341

BLAST of Cp4.1LG11g00140 vs. TAIR 10
Match: AT1G54440.1 (Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain )

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-08
Identity = 45/153 (29.41%), Postives = 67/153 (43.79%), Query Frame = 0

Query: 71  GSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIK 130
           G  C+MQI+     Y+VD  +  + +    +   +     KVIH   RD   L   FGI 
Sbjct: 151 GLTCLMQISTRTEDYIVDIFKLWDHIGPYLRELFKDPKKKKVIHGADRDIIWLQRDFGIY 210

Query: 131 LNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKF 190
           + N+ DT  A  ++       K   N + F+       YCGV+   KE            
Sbjct: 211 VCNLFDTGQASRVL-------KLERNSLEFL----LKHYCGVA-ANKE-------YQKAD 270

Query: 191 WTYRPLSELMVRAAADDVRFLLYIYHKLMEKLN 224
           W  RPL ++M R A +D  +LLYIY  +  +L+
Sbjct: 271 WRIRPLPDVMKRYAREDTHYLLYIYDVMRMELH 284

BLAST of Cp4.1LG11g00140 vs. TAIR 10
Match: AT1G54440.2 (Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain )

HSP 1 Score: 57.8 bits (138), Expect = 2.0e-08
Identity = 45/153 (29.41%), Postives = 67/153 (43.79%), Query Frame = 0

Query: 71  GSLCIMQIAFPDAIYLVDAVQGGEELVKVCKPALESTYVTKVIHDCKRDSEALYFQFGIK 130
           G  C+MQI+     Y+VD  +  + +    +   +     KVIH   RD   L   FGI 
Sbjct: 151 GLTCLMQISTRTEDYIVDIFKLWDHIGPYLRELFKDPKKKKVIHGADRDIIWLQRDFGIY 210

Query: 131 LNNVIDTQIAYSLIEEQEGWTKTPDNYISFVGLLADSRYCGVSYVEKEEVRLLLRQDPKF 190
           + N+ DT  A  ++       K   N + F+       YCGV+   KE            
Sbjct: 211 VCNLFDTGQASRVL-------KLERNSLEFL----LKHYCGVA-ANKE-------YQKAD 270

Query: 191 WTYRPLSELMVRAAADDVRFLLYIYHKLMEKLN 224
           W  RPL ++M R A +D  +LLYIY  +  +L+
Sbjct: 271 WRIRPLPDVMKRYAREDTHYLLYIYDVMRMELH 284

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8NHP72.6e-1630.81piRNA biogenesis protein EXD1 OS=Homo sapiens OX=9606 GN=EXD1 PE=1 SV=4[more]
Q8CDF71.3e-1531.79piRNA biogenesis protein EXD1 OS=Mus musculus OX=10090 GN=Exd1 PE=1 SV=1[more]
Q6NRD51.6e-1027.17piRNA biogenesis protein EXD1 OS=Xenopus laevis OX=8355 GN=exd1 PE=2 SV=1[more]
Q0P3U32.0e-0826.47piRNA biogenesis protein EXD1 OS=Danio rerio OX=7955 GN=exd1 PE=2 SV=1[more]
P569601.3e-0728.30Exosome component 10 OS=Mus musculus OX=10090 GN=Exosc10 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023545692.11.60e-254100.00piRNA biogenesis protein EXD1-like [Cucurbita pepo subsp. pepo][more]
KAG6598308.18.88e-25299.12piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7029279.11.26e-25198.82piRNA biogenesis protein EXD1, partial [Cucurbita argyrosperma subsp. argyrosper... [more]
XP_022997512.13.34e-24998.82piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita maxima][more]
XP_022961700.11.59e-24798.24piRNA biogenesis protein EXD1-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1K5891.62e-24998.82piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HD177.69e-24898.24piRNA biogenesis protein EXD1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1KE471.43e-23798.76piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1HB374.09e-23798.45piRNA biogenesis protein EXD1-like isoform X3 OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1K7P21.64e-23698.45uncharacterized protein LOC111492408 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
Match NameE-valueIdentityDescription
AT2G25910.11.7e-14570.003'-5' exonuclease domain-containing protein / K homology domain-containing prote... [more]
AT2G25910.24.3e-14469.793'-5' exonuclease domain-containing protein / K homology domain-containing prote... [more]
AT1G54440.12.0e-0829.41Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain [more]
AT1G54440.22.0e-0829.41Polynucleotidyl transferase, ribonuclease H fold protein with HRDC domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR0025623'-5' exonuclease domainSMARTSM0047435exoneu6coord: 34..223
e-value: 1.2E-24
score: 97.9
IPR0025623'-5' exonuclease domainPFAMPF01612DNA_pol_A_exo1coord: 46..222
e-value: 5.4E-23
score: 81.6
IPR004087K Homology domainSMARTSM00322kh_6coord: 270..338
e-value: 9.6E-8
score: 41.7
IPR036612K Homology domain, type 1 superfamilyGENE3D3.30.1370.10K Homology domain, type 1coord: 261..337
e-value: 4.0E-10
score: 41.2
IPR036612K Homology domain, type 1 superfamilySUPERFAMILY54791Eukaryotic type KH-domain (KH-domain type I)coord: 269..337
IPR004088K Homology domain, type 1PFAMPF00013KH_1coord: 276..334
e-value: 1.9E-9
score: 37.2
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 3..237
e-value: 3.8E-41
score: 143.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR46814:SF4SUBFAMILY NOT NAMEDcoord: 6..337
NoneNo IPR availablePANTHERPTHR46814EGALITARIAN, ISOFORM Bcoord: 6..337
NoneNo IPR availablePROSITEPS50084KH_TYPE_1coord: 271..333
score: 11.594319
NoneNo IPR availableCDDcd06148Egl_like_exocoord: 47..242
e-value: 1.74584E-78
score: 236.797
NoneNo IPR availableCDDcd00105KH-Icoord: 276..333
e-value: 3.36944E-9
score: 50.6363
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 28..244

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG11g00140.1Cp4.1LG11g00140.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0006139 nucleobase-containing compound metabolic process
molecular_function GO:0008408 3'-5' exonuclease activity
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding