Cp4.1LG10g09680 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g09680 vs. ExPASy Swiss-Prot
Match: Q8RJP2 (Rhamnogalacturonate lyase OS=Dickeya dadantii (strain 3937) OX=198628 GN=rhiE PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 7.9e-24
Identity = 121/434 (27.88%), Postives = 181/434 (41.71%), Query Frame = 0

Query: 199 GEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLA 258
           G V  KY ++   ++    G +    N  G W+V  S E+ +G   KQ L VH     L 
Sbjct: 200 GSVYSKYDFAGYQRESRYWGVMG---NGYGAWMVPASGEYYSGDALKQELLVHQDAIILN 259

Query: 259 MFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNS------TSDVSEAHN--------LW 318
              G+H+   D++        + K++GP L+Y+N        +DVS             W
Sbjct: 260 YLTGSHFGTPDMVAQ----PGFEKLYGPWLLYINQGNDRELVADVSRRAEHERASWPYRW 319

Query: 319 IDAKEQENERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQT 378
           +D      +R ++SGRL  +          A  A V L+++ E   + I++  Y F  +T
Sbjct: 320 LDDARYPRQRATVSGRLRTE----------APHATVVLNSSAE--NFDIQTTGYLFSART 379

Query: 379 DSNGDFTIRNVIPGVYGLHGWVPG--FIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPT 438
           + +G F++ NV PG Y L  +  G   IG  L Q  V V  G  T LG +     +  P 
Sbjct: 380 NRDGRFSLSNVPPGEYRLSAYADGGTQIG-LLAQQTVRVE-GKKTRLGQI--DARQPAPL 439

Query: 439 VWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGIN 498
            W IG  DR A  F   D               K RQY  W+         D  F +G +
Sbjct: 440 AWAIGQADRRADEFRFGD---------------KPRQY-RWQ----TEVPADLTFEIGKS 499

Query: 499 DPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVNSM 558
             +KDW+YAQ     + G     +W I FN T   E  Y L +AIA+A+ + +    +S 
Sbjct: 500 RERKDWYYAQT----QPG-----SWHILFN-TRTPEQPYTLNIAIAAASNNGMTTPASSP 559

Query: 559 GSESSLVFQL---MNLGMDNTVCRHG-NHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGD 611
                L  QL   +    D ++ R     G Y    + +P+  L +G N I L       
Sbjct: 560 QLAVKLNGQLLTTLKYDNDKSIYRGAMQSGRYHEAHIPLPAGALQQGGNRITL------- 573

BLAST of Cp4.1LG10g09680 vs. ExPASy Swiss-Prot
Match: Q0C7K7 (Probable rhamnogalacturonate lyase B OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) OX=341663 GN=rglB PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 2.2e-18
Identity = 102/443 (23.02%), Postives = 186/443 (41.99%), Query Frame = 0

Query: 114 YIMRTGMPGFYCYAIYEHPSACRAF--DLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPE 173
           + +R G  G + ++   + +    F  +L + R +F+   + + ++  S+   +  P+P 
Sbjct: 123 WFLRDGETGLHMFSRLAYYNETTPFLRNLQEFRTLFRPNTDLWTHLTSSE--LQTAPLPS 182

Query: 174 DLHPGRGEELIVPESVLLVNPINPDLK----GEVDDKYQYSVDNKDGGVHGWI----SSS 233
           D     G++++V ++    N    D       E   KY +S   +D  VHG      +S+
Sbjct: 183 D--EAVGKQVVVQDATWRFNNTPNDAYYTQFSEYFTKYTFSNAWRDNNVHGLYADGSTSN 242

Query: 234 PNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKV 293
               G W+V  + +   GGP   +LTV  G     +    H  G   +T+      + + 
Sbjct: 243 GTTFGAWLVMNTKDTYYGGPLHSDLTV-DGIVYNYIVSNHHGEGTPNITN-----GFDRT 302

Query: 294 FGPVLVYLN-----STSDV-SEAHNL----W-----------IDAKEQENERGSISGRLL 353
           FGP     N     S +++ SEA +L    W           +      ++RGS+ G++ 
Sbjct: 303 FGPQFYLFNGGGSSSLNELRSEAESLADPSWNVEFYDSIAKHVVGYVPSSKRGSVQGQIK 362

Query: 354 VQDRFISSSPIPARDAHVGLSAAREEGGW----QIESKEYQFWVQTDSNGDFTIRNVIPG 413
           +            R A   ++    +G +     ++ + YQ+WV+ D+NG F + +V+ G
Sbjct: 363 L-----------PRGATRPIAILTVDGQYFQDNSVDPRSYQYWVEMDANGKFQLDHVVEG 422

Query: 414 VYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSF-Y 473
            Y L  +  G  GDY+    V V     T +   ++ P   G  VW +G PD+++G F +
Sbjct: 423 KYRLTVYADGIFGDYVRDG-VQVRGRKTTRIND-SWQPESAGVEVWRLGTPDKSSGEFLH 482

Query: 474 VPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKD-----WFYAQ 515
               +P +     LH PE    +G ++   D    N   +T+G +DP  D     W    
Sbjct: 483 GVARDPTHP----LHPPEYLIYWGAYDWQQDFP--NGVNYTIGSSDPATDFNTVHWSVFG 536

BLAST of Cp4.1LG10g09680 vs. ExPASy Swiss-Prot
Match: Q5B5P1 (Probable rhamnogalacturonate lyase C OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglC PE=3 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 6.5e-18
Identity = 116/551 (21.05%), Postives = 203/551 (36.84%), Query Frame = 0

Query: 7   LRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDINWSWPG 66
           L   +  +H  + N    V ++   G+V        D L  +  +  K  Y D + +  G
Sbjct: 23  LTTTSNSTHYTISNSRFSVAVAKSNGHVVDANLDGQDLLGPLSGNSGKGPYLDCSCTPEG 82

Query: 67  GKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIM----RTGMPG 126
                    G+E  ++N TD T       +   +       LS   +Y+      TG+  
Sbjct: 83  -----FWTPGAEPALVNGTDSTGTPYVGVIMTDTYETTNQTLS---QYLFLRGEETGLHA 142

Query: 127 FYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELI 186
           F     Y + S      L + R +F+     + + + S+     MP+         E++ 
Sbjct: 143 F-SRVTYYNESDYFLRGLGELRTLFRPNTNLWTHFSGSEGNYGPMPLSST------EKIT 202

Query: 187 VPESVLLVNPINPD----LKGEVDDKYQYSVDNKDGGVHGWISSSP-----NNIGFWVVF 246
           V ++   +     D       +   KY  +   +D  VHG  S+       N  G W+V 
Sbjct: 203 VQDATTYLGDTTDDPYVSQYSDYFTKYTLTESWRDHDVHGHFSNGSTSGDGNTYGAWLVH 262

Query: 247 PSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNS 306
            + E   GGP   +L V  G     +  G +      LTH      + + FGP   + NS
Sbjct: 263 NTRETYYGGPLHADLVV-DGIVYNYIVSGHYGAPNPNLTH-----GFDRTFGPQYYHFNS 322

Query: 307 TSDVSEAHNLWIDAKEQENERGSI----------------SGRLLVQDRFISSSPIPARD 366
               +    L  DA +  +   +                 +GR   + +   + P  A+ 
Sbjct: 323 GGPGTTLEELRADAAQYASPEWNAEFYDSIAKHIPNYVPSTGRTTFRGKV--NLPKGAKK 382

Query: 367 AHVGLSAAREEGGWQIESKE-YQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQ 426
             + LS   ++    +  K+  Q+W + D +G FTI  V+ G Y +  +     G ++  
Sbjct: 383 PIIVLSENEQDFQLNVFKKDSLQYWAEIDGSGAFTIPRVVKGTYRVTIYADEIFGWFIKD 442

Query: 427 SLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSF---YVPDVNPMYVNKLFLHS 486
           ++  + + +HT     T+     G  +W IG PD+++G F   Y PD +           
Sbjct: 443 NVKVIGSNAHT----FTWKEETAGKEIWRIGVPDKSSGEFLHGYAPDTSKPL-------Q 502

Query: 487 PEKFRQYGLWEGYSDLHPINDQV-FTVGINDPKKDWFYAQVCRRGEDGKFV--------A 516
           PE++R Y  W  Y       + V + VG +DP KD  Y         G  +         
Sbjct: 503 PEQYRIY--WGKYDYPSDFPEGVNYHVGKSDPAKDLNYIHWSFFPSQGNHLRNEPYYQNV 537

BLAST of Cp4.1LG10g09680 vs. ExPASy Swiss-Prot
Match: Q5AZ85 (Rhamnogalacturonate lyase B OS=Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) OX=227321 GN=rglB PE=2 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 5.5e-17
Identity = 93/407 (22.85%), Postives = 162/407 (39.80%), Query Frame = 0

Query: 114 YIMRTGMPGFYCYAIYEHPSACRAF--DLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPE 173
           + +R G  GF+ ++   + +    F  +L + R +F+   + + ++  SD +   +P  E
Sbjct: 123 WFLRDGETGFHMFSRLAYYNETTPFLRNLQELRTLFRPNTDLWTHLTSSDLQTAPLPSDE 182

Query: 174 DLHPGRGEELIVPESVLLVNPINPDLK----GEVDDKYQYSVDNKDGGVHGWI----SSS 233
            +     E+++V ++   +N    D       E   KY +S   +D  VHG      +S 
Sbjct: 183 AI----AEQIVVQDATWRLNNTPDDAYYQQFSEYFTKYTFSNHWRDNDVHGLYADGSTSD 242

Query: 234 PNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKV 293
               G W+V  + +   GGP   +LTV  G     +    H  G   +T+      + + 
Sbjct: 243 GTTYGAWLVMNTKDTYYGGPLHSDLTV-DGIIYNYIVSNHHGEGTPNITN-----GFDRT 302

Query: 294 FGPVLVYLNSTSDVS------EAHNL----W-----------IDAKEQENERGSISGRLL 353
           FGP     N     S      EA +L    W           +      ++RGS+ G + 
Sbjct: 303 FGPQFYLFNGGGSSSLEELRDEARSLASPSWNADFYDSIAKHVIGYVPSSQRGSVKGTIK 362

Query: 354 VQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGL 413
           +     S   +   D H     +            +Q+W   D NG F+I  V+ G Y L
Sbjct: 363 LPKNAKSPIAVLTVDGHYFQDNS-------AVPSSHQYWADIDKNGRFSIDRVVAGKYRL 422

Query: 414 HGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSF-YVPDV 473
             +  G  GD+    +V V A   T +   T+ P   G  +W +G PD+++G F +    
Sbjct: 423 TVYADGIFGDFTRDGIV-VKARKSTSIK-ETWKPESAGTEIWRLGTPDKSSGEFRHGAAR 482

Query: 474 NPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDW 489
           +P +      H PE    +G ++  SD     D  + +G +DP  D+
Sbjct: 483 DPTHPR----HPPEYLIYWGAYDWQSDFPGGID--YMIGESDPATDF 504

BLAST of Cp4.1LG10g09680 vs. ExPASy Swiss-Prot
Match: A5ABH4 (Probable rhamnogalacturonate lyase B OS=Aspergillus niger (strain CBS 513.88 / FGSC A1513) OX=425011 GN=rglB PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 7.2e-17
Identity = 89/402 (22.14%), Postives = 168/402 (41.79%), Query Frame = 0

Query: 114 YIMRTGMPGFYCYA--IYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPE 173
           + +R G  G + ++   Y + +     +L + R +F+   E + +++ S+ +   +P  +
Sbjct: 124 WFLRDGETGLHTFSRLAYYNETTPYLRNLQEFRTLFRPNTELWTHLSSSEVQTAPLPSKK 183

Query: 174 DLHPGRGEELIVPESVLLVNPINPD----LKGEVDDKYQYSVDNKDGGVHGWI----SSS 233
            +     EE++V ++    N    D       +   KY +S   +D  VHG      +S+
Sbjct: 184 AVE----EEVVVQDATWTFNNTPTDEYYVQFADYFTKYTFSNAWRDNSVHGMYADGSTSN 243

Query: 234 PNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKV 293
            +  G W+V  + +   GGP   +LTV  G     +    H  G   +T+      + + 
Sbjct: 244 GSTFGAWLVMNTKDTYYGGPLHSDLTV-DGIVYNYLVSNHHGEGTPNITY-----GFDRT 303

Query: 294 FGPVLVYLN-STSDVSEAHNLWIDAKEQENERGSISGRLLVQDRFISSSPIPARDAHVG- 353
           FGP   + N      +    L  DA+   +   ++     +    +  +P   R +  G 
Sbjct: 304 FGPQYYHFNGGKGSTASLQELKSDAETLADPSWNVDFYDSIAKHVVGYTPSSQRGSVQGK 363

Query: 354 ----------LSAAREEGGW----QIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVP 413
                     ++    +G +     + S  YQ+W + D +G F++ +V  G Y L  +  
Sbjct: 364 IKLPKGATRPIAVLTVDGQYFQDNSVNSSSYQYWAEIDDSGHFSVDHVKEGPYRLTVYAD 423

Query: 414 GFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSF-YVPDVNPMYV 473
           G  GD++    V V AG  T +   T+     G  +W +G PD+++G F +    +P + 
Sbjct: 424 GIFGDFVRDG-VQVKAGKKTTIQ-ETWEAESAGTEIWRLGTPDKSSGEFRHGVARDPTHP 483

Query: 474 NKLFLHSPEKFRQYGLWEGYSDL-HPINDQVFTVGINDPKKD 488
               LH PE    +G ++  SD    IN   +T+G +DP  D
Sbjct: 484 ----LHPPEYLIYWGAYDWQSDFPDGIN---YTIGTSDPATD 506

BLAST of Cp4.1LG10g09680 vs. NCBI nr
Match: KAG7033955.1 (rhiE, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1281 bits (3314), Expect = 0.0
Identity = 613/639 (95.93%), Postives = 618/639 (96.71%), Query Frame = 0

Query: 1   MGKKKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI 60
           MGKKKKLRLQNQGSHVILDNGLL+VTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI
Sbjct: 39  MGKKKKLRLQNQGSHVILDNGLLVVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI 98

Query: 61  NWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGM 120
           NWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGM
Sbjct: 99  NWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGM 158

Query: 121 PGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEE 180
           PGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEE
Sbjct: 159 PGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEE 218

Query: 181 LIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRN 240
           LIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRN
Sbjct: 219 LIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRN 278

Query: 241 GGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEA 300
           GGPTKQNLTVHTGPTCLAMFHGTHYIGEDI+THIKEGEAWRKVFGPVLVYLNSTSDVSEA
Sbjct: 279 GGPTKQNLTVHTGPTCLAMFHGTHYIGEDIITHIKEGEAWRKVFGPVLVYLNSTSDVSEA 338

Query: 301 HNLWIDAKEQ--------------------ENERGSISGRLLVQDRFISSSPIPARDAHV 360
           HNLWIDAKEQ                    ENERGSISGRLLVQDRFISSSPIPARDAHV
Sbjct: 339 HNLWIDAKEQRMLEETAWPYNFVSSSFYFKENERGSISGRLLVQDRFISSSPIPARDAHV 398

Query: 361 GLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVT 420
           GLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVT
Sbjct: 399 GLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVT 458

Query: 421 VSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQY 480
           VSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTA SFYVPDVNPMYVNKLFLHSPEKFRQY
Sbjct: 459 VSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTADSFYVPDVNPMYVNKLFLHSPEKFRQY 518

Query: 481 GLWEGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGT 540
           GLWEGYSDLHPINDQVFTVGINDPKKDWF+AQVCRRGEDGKFVATTWSIKFNMTSLTEGT
Sbjct: 519 GLWEGYSDLHPINDQVFTVGINDPKKDWFFAQVCRRGEDGKFVATTWSIKFNMTSLTEGT 578

Query: 541 YRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSS 600
           YRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVE+PSS
Sbjct: 579 YRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEIPSS 638

Query: 601 MLIKGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTPSS 619
           MLIKGDN+IFLTQARGGDELCGLLYDYLRLEAPDDTPSS
Sbjct: 639 MLIKGDNSIFLTQARGGDELCGLLYDYLRLEAPDDTPSS 677

BLAST of Cp4.1LG10g09680 vs. NCBI nr
Match: KAG6603784.1 (hypothetical protein SDJN03_04393, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1245 bits (3221), Expect = 0.0
Identity = 605/666 (90.84%), Postives = 611/666 (91.74%), Query Frame = 0

Query: 1   MGKKKKLRLQNQGSHV---------------------------ILDNGLLMVTISNPQGY 60
           MGKKKKLRLQNQGSHV                           ILDNGLL VTISNPQGY
Sbjct: 39  MGKKKKLRLQNQGSHVSFEQPYDSCFLVIRFSVILVCYDSLQVILDNGLLAVTISNPQGY 98

Query: 61  VSGIKYGNMDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISF 120
           VSGIKYGNMDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISF
Sbjct: 99  VSGIKYGNMDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISF 158

Query: 121 RKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEK 180
           RKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSACRAFDLVQTRM     Q +
Sbjct: 159 RKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSACRAFDLVQTRM----GQIR 218

Query: 181 FHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKD 240
           FHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKD
Sbjct: 219 FHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKD 278

Query: 241 GGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTH 300
           GGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDI+TH
Sbjct: 279 GGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDIITH 338

Query: 301 IKEGEAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQ--------------------ENE 360
           IKEGEAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQ                    ENE
Sbjct: 339 IKEGEAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQRMLEETAWPYNFVSSSFYFKENE 398

Query: 361 RGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIR 420
           RGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIR
Sbjct: 399 RGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIR 458

Query: 421 NVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTA 480
           NVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTA
Sbjct: 459 NVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTA 518

Query: 481 GSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFYAQV 540
            SFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWF+AQV
Sbjct: 519 DSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFFAQV 578

Query: 541 CRRGEDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVNSMGSESSLVFQLM 600
           CRRGEDGKFVATTWS+KFNMTSLTEGTYRLRLAIASATRSDLKINVNSMGSESSLVFQLM
Sbjct: 579 CRRGEDGKFVATTWSVKFNMTSLTEGTYRLRLAIASATRSDLKINVNSMGSESSLVFQLM 638

Query: 601 NLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGDELCGLLYDYLRLEAP 619
           NLGMDNTVCRHGNHGLYRNYSVE+PSSMLIKGDN+IFLTQARGGDELCGLLYDYLRLEAP
Sbjct: 639 NLGMDNTVCRHGNHGLYRNYSVEIPSSMLIKGDNSIFLTQARGGDELCGLLYDYLRLEAP 698

BLAST of Cp4.1LG10g09680 vs. NCBI nr
Match: XP_023544015.1 (probable rhamnogalacturonate lyase C [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1225 bits (3170), Expect = 0.0
Identity = 591/619 (95.48%), Postives = 592/619 (95.64%), Query Frame = 0

Query: 1   MGKKKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI 60
           MGKKKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI
Sbjct: 1   MGKKKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI 60

Query: 61  NWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGM 120
           NWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRK    S+R           YIMRTGM
Sbjct: 61  NWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKASKLSSR-----------YIMRTGM 120

Query: 121 PGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEE 180
           PGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEE
Sbjct: 121 PGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEE 180

Query: 181 LIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRN 240
           LIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRN
Sbjct: 181 LIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRN 240

Query: 241 GGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEA 300
           GGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEA
Sbjct: 241 GGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEA 300

Query: 301 HNLWIDAKEQENERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQF 360
           HNLWIDAKEQENERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQF
Sbjct: 301 HNLWIDAKEQENERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQF 360

Query: 361 WVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDG 420
           WVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDG
Sbjct: 361 WVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDG 420

Query: 421 PTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVG 480
           PTVWEIGFPDRTAGSFYVPDVNPM            FRQYGLWEGYSDLHPINDQVFTVG
Sbjct: 421 PTVWEIGFPDRTAGSFYVPDVNPM------------FRQYGLWEGYSDLHPINDQVFTVG 480

Query: 481 INDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVN 540
           INDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVN
Sbjct: 481 INDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVN 540

Query: 541 SMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGDEL 600
           SMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGDEL
Sbjct: 541 SMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGDEL 596

Query: 601 CGLLYDYLRLEAPDDTPSS 619
           CGLLYDYLRLEAPDDTPSS
Sbjct: 601 CGLLYDYLRLEAPDDTPSS 596

BLAST of Cp4.1LG10g09680 vs. NCBI nr
Match: XP_011658136.1 (probable rhamnogalacturonate lyase B isoform X1 [Cucumis sativus])

HSP 1 Score: 1197 bits (3098), Expect = 0.0
Identity = 566/634 (89.27%), Postives = 591/634 (93.22%), Query Frame = 0

Query: 4   KKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDINWS 63
           +KKLRLQNQGSHVILDNGLL VTISNPQGYV GI YG MDNLLDVKSSESKRGYWDINWS
Sbjct: 3   RKKLRLQNQGSHVILDNGLLKVTISNPQGYVIGITYGGMDNLLDVKSSESKRGYWDINWS 62

Query: 64  WPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGF 123
           WPGGKDRYQLLKGSEFNVIN++DDTIEISFRK F+ STRGNKLPL VDIRYIMRTG+PGF
Sbjct: 63  WPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDFDLSTRGNKLPLGVDIRYIMRTGIPGF 122

Query: 124 YCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIV 183
           YCYAIYEHPS CRAFDL QTRMVFKLRQEKFHYMAISDEKQRIMPMPEDL PGRGEELIV
Sbjct: 123 YCYAIYEHPSECRAFDLAQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLRPGRGEELIV 182

Query: 184 PESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGP 243
           PESVLLVNPINPDLKGEVDDKYQYS DNKDGGVHGWISSSPNNIGFW+VFPSHEFRNGGP
Sbjct: 183 PESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVHGWISSSPNNIGFWIVFPSHEFRNGGP 242

Query: 244 TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEAHNL 303
           TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGP+LVYLNSTSDVSEAHNL
Sbjct: 243 TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPILVYLNSTSDVSEAHNL 302

Query: 304 WIDAKEQE--------------------NERGSISGRLLVQDRFISSSPIPARDAHVGLS 363
           WIDAKEQ                      ERGSISGRLLV+DRF+SSSPIPARDAH+GLS
Sbjct: 303 WIDAKEQRMQEETAWPYNFVASSFYLMARERGSISGRLLVRDRFVSSSPIPARDAHIGLS 362

Query: 364 AAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSA 423
           AAREEG WQIESKEYQFWV+TDSNGDFTIRN+IPGVYGLHGWVPGFIGDYLH+SLVTVSA
Sbjct: 363 AAREEGAWQIESKEYQFWVKTDSNGDFTIRNIIPGVYGLHGWVPGFIGDYLHKSLVTVSA 422

Query: 424 GSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLW 483
           GS+ HLGILTYSP RDGPTVWEIGFPDRTA SFYVPDVNPMYVNKLFLHSPEKFRQYGLW
Sbjct: 423 GSYNHLGILTYSPPRDGPTVWEIGFPDRTANSFYVPDVNPMYVNKLFLHSPEKFRQYGLW 482

Query: 484 EGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRL 543
           EGYSDLHP NDQ+FTVGINDPKKDWF+AQVCRRGEDGK+VATTW+IKFNMTSLT+GTYRL
Sbjct: 483 EGYSDLHPRNDQIFTVGINDPKKDWFFAQVCRRGEDGKYVATTWTIKFNMTSLTDGTYRL 542

Query: 544 RLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLI 603
           RL+IASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYR YS+++PSSML+
Sbjct: 543 RLSIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRIYSIDIPSSMLV 602

Query: 604 KGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTP 617
           KGDN IFLTQAR GD LCG+LYDYLRLEAPD TP
Sbjct: 603 KGDNCIFLTQARNGDPLCGILYDYLRLEAPDATP 636

BLAST of Cp4.1LG10g09680 vs. NCBI nr
Match: XP_031742930.1 (probable rhamnogalacturonate lyase B isoform X2 [Cucumis sativus])

HSP 1 Score: 1191 bits (3081), Expect = 0.0
Identity = 565/634 (89.12%), Postives = 590/634 (93.06%), Query Frame = 0

Query: 4   KKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDINWS 63
           +KKLRLQNQGSHVILDNGLL VTISNPQGYV GI YG MDNLLDVKSSESKRGYWDINWS
Sbjct: 3   RKKLRLQNQGSHVILDNGLLKVTISNPQGYVIGITYGGMDNLLDVKSSESKRGYWDINWS 62

Query: 64  WPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGF 123
           WPGGKDRYQLLKGSEFNVIN++DDTIEISFRK F+ STRGNKLPL VDIRYIMRTG+PGF
Sbjct: 63  WPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDFDLSTRGNKLPLGVDIRYIMRTGIPGF 122

Query: 124 YCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIV 183
           YCYAIYEHPS CRAFDL QTRMVFKLRQEKFHYMAISDEKQRIMPMPEDL PGRGEELIV
Sbjct: 123 YCYAIYEHPSECRAFDLAQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLRPGRGEELIV 182

Query: 184 PESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGP 243
           PESVLLVNPINPDLKGEVDDKYQYS DNKDGGVHGWISSSPNNIGFW+VFPSHEFRNGGP
Sbjct: 183 PESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVHGWISSSPNNIGFWIVFPSHEFRNGGP 242

Query: 244 TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEAHNL 303
           TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGP+LVYLNSTSDVSEAHNL
Sbjct: 243 TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPILVYLNSTSDVSEAHNL 302

Query: 304 WIDAKEQE--------------------NERGSISGRLLVQDRFISSSPIPARDAHVGLS 363
           WIDAKEQ                      ERGSISGRLLV+DRF+SSSPIPARDAH+GLS
Sbjct: 303 WIDAKEQRMQEETAWPYNFVASSFYLMARERGSISGRLLVRDRFVSSSPIPARDAHIGLS 362

Query: 364 AAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSA 423
           AAREEG WQIESKEYQFWV+TDSNGDFTIRN+IPGVYGLHGWVPGFIGDYLH+SLVTVSA
Sbjct: 363 AAREEGAWQIESKEYQFWVKTDSNGDFTIRNIIPGVYGLHGWVPGFIGDYLHKSLVTVSA 422

Query: 424 GSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLW 483
           GS+ HLGILTYSP RDGPTVWEIGFPDRTA SFYVPDVNPMYVNKLFLHSPEKFRQYGLW
Sbjct: 423 GSYNHLGILTYSPPRDGPTVWEIGFPDRTANSFYVPDVNPMYVNKLFLHSPEKFRQYGLW 482

Query: 484 EGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRL 543
           EGYSDLHP NDQ+FTVGINDPKKDWF+AQVCR GEDGK+VATTW+IKFNMTSLT+GTYRL
Sbjct: 483 EGYSDLHPRNDQIFTVGINDPKKDWFFAQVCR-GEDGKYVATTWTIKFNMTSLTDGTYRL 542

Query: 544 RLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLI 603
           RL+IASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYR YS+++PSSML+
Sbjct: 543 RLSIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRIYSIDIPSSMLV 602

Query: 604 KGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTP 617
           KGDN IFLTQAR GD LCG+LYDYLRLEAPD TP
Sbjct: 603 KGDNCIFLTQARNGDPLCGILYDYLRLEAPDATP 635

BLAST of Cp4.1LG10g09680 vs. ExPASy TrEMBL
Match: A0A1S4DU69 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103484616 PE=3 SV=1)

HSP 1 Score: 1185 bits (3066), Expect = 0.0
Identity = 560/634 (88.33%), Postives = 589/634 (92.90%), Query Frame = 0

Query: 4   KKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDINWS 63
           KKKLRLQNQGSHVILDNGLL VTISNPQGYV GI YG MDN+LDVKSSESKRGYWDINWS
Sbjct: 3   KKKLRLQNQGSHVILDNGLLKVTISNPQGYVIGITYGGMDNILDVKSSESKRGYWDINWS 62

Query: 64  WPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGF 123
           WPGGKDRYQLLKGSEFNVIN++DDTIEISFRK F+ STRGNKLPL VDIRYIMRTG+PGF
Sbjct: 63  WPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDFDLSTRGNKLPLGVDIRYIMRTGIPGF 122

Query: 124 YCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIV 183
           YCYAIYEHPS CRAFDL QTRMVFKLRQEKFHYMAISDEKQRIMPMPEDL PGRGE+LIV
Sbjct: 123 YCYAIYEHPSECRAFDLAQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLRPGRGEQLIV 182

Query: 184 PESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGP 243
           PESVLLVNPINPDLKGEVDDKYQYS DNKDGGVHGWISSSPNNIGFW+VFPS+EFRNGGP
Sbjct: 183 PESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVHGWISSSPNNIGFWIVFPSYEFRNGGP 242

Query: 244 TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEAHNL 303
           TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGP+L+YLNSTSDVSEAHNL
Sbjct: 243 TKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPILIYLNSTSDVSEAHNL 302

Query: 304 WIDAKEQE--------------------NERGSISGRLLVQDRFISSSPIPARDAHVGLS 363
           WIDAKEQ                      ERGSISGRLLV+DRFISSSPIPARDAHVGLS
Sbjct: 303 WIDAKEQRMQEETAWPYNFVSSSFYLMARERGSISGRLLVRDRFISSSPIPARDAHVGLS 362

Query: 364 AAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSA 423
           AAREEG WQ ESKEYQFWV+TDSNGDFTIRN+IPGVYGLHGWVPGFIGDYLH+SLVTVSA
Sbjct: 363 AAREEGAWQTESKEYQFWVKTDSNGDFTIRNIIPGVYGLHGWVPGFIGDYLHKSLVTVSA 422

Query: 424 GSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLW 483
           GS+THLGILTYSPLRDGPTVWEIGFPDRTA SFYVPDVNPMYVNKLFLHSPEKFRQYGLW
Sbjct: 423 GSYTHLGILTYSPLRDGPTVWEIGFPDRTANSFYVPDVNPMYVNKLFLHSPEKFRQYGLW 482

Query: 484 EGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRL 543
           +GYSD HP NDQ+FTVGINDPKKDWF+AQVCRRGEDGK+VATTW+IKFNM SLT+G YRL
Sbjct: 483 DGYSDSHPRNDQIFTVGINDPKKDWFFAQVCRRGEDGKYVATTWTIKFNMKSLTDGIYRL 542

Query: 544 RLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLI 603
           RL+IASATRSDLKINVNSM SESSLVFQLM+LGMDNTVCRHGNHGLYR Y+++VPSSML+
Sbjct: 543 RLSIASATRSDLKINVNSMESESSLVFQLMDLGMDNTVCRHGNHGLYRIYTIDVPSSMLV 602

Query: 604 KGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTP 617
           KGDN+IFLTQAR GD LCG+LYDYLRLEAPD TP
Sbjct: 603 KGDNSIFLTQARNGDALCGILYDYLRLEAPDATP 636

BLAST of Cp4.1LG10g09680 vs. ExPASy TrEMBL
Match: A0A1S4DTF3 (Rhamnogalacturonan endolyase OS=Cucumis melo OX=3656 GN=LOC103484616 PE=3 SV=1)

HSP 1 Score: 1171 bits (3029), Expect = 0.0
Identity = 560/660 (84.85%), Postives = 589/660 (89.24%), Query Frame = 0

Query: 4   KKKLRLQNQGSHV--------------------------ILDNGLLMVTISNPQGYVSGI 63
           KKKLRLQNQGSHV                          ILDNGLL VTISNPQGYV GI
Sbjct: 3   KKKLRLQNQGSHVSLEPYDSYVFVNMFYVIHVCFDFLQVILDNGLLKVTISNPQGYVIGI 62

Query: 64  KYGNMDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVF 123
            YG MDN+LDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVIN++DDTIEISFRK F
Sbjct: 63  TYGGMDNILDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDF 122

Query: 124 NPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYM 183
           + STRGNKLPL VDIRYIMRTG+PGFYCYAIYEHPS CRAFDL QTRMVFKLRQEKFHYM
Sbjct: 123 DLSTRGNKLPLGVDIRYIMRTGIPGFYCYAIYEHPSECRAFDLAQTRMVFKLRQEKFHYM 182

Query: 184 AISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVH 243
           AISDEKQRIMPMPEDL PGRGE+LIVPESVLLVNPINPDLKGEVDDKYQYS DNKDGGVH
Sbjct: 183 AISDEKQRIMPMPEDLRPGRGEQLIVPESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVH 242

Query: 244 GWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEG 303
           GWISSSPNNIGFW+VFPS+EFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEG
Sbjct: 243 GWISSSPNNIGFWIVFPSYEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEG 302

Query: 304 EAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQE--------------------NERGSI 363
           EAWRKVFGP+L+YLNSTSDVSEAHNLWIDAKEQ                      ERGSI
Sbjct: 303 EAWRKVFGPILIYLNSTSDVSEAHNLWIDAKEQRMQEETAWPYNFVSSSFYLMARERGSI 362

Query: 364 SGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIP 423
           SGRLLV+DRFISSSPIPARDAHVGLSAAREEG WQ ESKEYQFWV+TDSNGDFTIRN+IP
Sbjct: 363 SGRLLVRDRFISSSPIPARDAHVGLSAAREEGAWQTESKEYQFWVKTDSNGDFTIRNIIP 422

Query: 424 GVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFY 483
           GVYGLHGWVPGFIGDYLH+SLVTVSAGS+THLGILTYSPLRDGPTVWEIGFPDRTA SFY
Sbjct: 423 GVYGLHGWVPGFIGDYLHKSLVTVSAGSYTHLGILTYSPLRDGPTVWEIGFPDRTANSFY 482

Query: 484 VPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRG 543
           VPDVNPMYVNKLFLHSPEKFRQYGLW+GYSD HP NDQ+FTVGINDPKKDWF+AQVCRRG
Sbjct: 483 VPDVNPMYVNKLFLHSPEKFRQYGLWDGYSDSHPRNDQIFTVGINDPKKDWFFAQVCRRG 542

Query: 544 EDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGM 603
           EDGK+VATTW+IKFNM SLT+G YRLRL+IASATRSDLKINVNSM SESSLVFQLM+LGM
Sbjct: 543 EDGKYVATTWTIKFNMKSLTDGIYRLRLSIASATRSDLKINVNSMESESSLVFQLMDLGM 602

Query: 604 DNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTP 617
           DNTVCRHGNHGLYR Y+++VPSSML+KGDN+IFLTQAR GD LCG+LYDYLRLEAPD TP
Sbjct: 603 DNTVCRHGNHGLYRIYTIDVPSSMLVKGDNSIFLTQARNGDALCGILYDYLRLEAPDATP 662

BLAST of Cp4.1LG10g09680 vs. ExPASy TrEMBL
Match: A0A1S3B0U4 (probable rhamnogalacturonate lyase B isoform X3 OS=Cucumis melo OX=3656 GN=LOC103484616 PE=4 SV=1)

HSP 1 Score: 1119 bits (2894), Expect = 0.0
Identity = 526/596 (88.26%), Postives = 555/596 (93.12%), Query Frame = 0

Query: 42  MDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPST 101
           MDN+LDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVIN++DDTIEISFRK F+ ST
Sbjct: 1   MDNILDVKSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDFDLST 60

Query: 102 RGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISD 161
           RGNKLPL VDIRYIMRTG+PGFYCYAIYEHPS CRAFDL QTRMVFKLRQEKFHYMAISD
Sbjct: 61  RGNKLPLGVDIRYIMRTGIPGFYCYAIYEHPSECRAFDLAQTRMVFKLRQEKFHYMAISD 120

Query: 162 EKQRIMPMPEDLHPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWIS 221
           EKQRIMPMPEDL PGRGE+LIVPESVLLVNPINPDLKGEVDDKYQYS DNKDGGVHGWIS
Sbjct: 121 EKQRIMPMPEDLRPGRGEQLIVPESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVHGWIS 180

Query: 222 SSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWR 281
           SSPNNIGFW+VFPS+EFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWR
Sbjct: 181 SSPNNIGFWIVFPSYEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWR 240

Query: 282 KVFGPVLVYLNSTSDVSEAHNLWIDAKEQE--------------------NERGSISGRL 341
           KVFGP+L+YLNSTSDVSEAHNLWIDAKEQ                      ERGSISGRL
Sbjct: 241 KVFGPILIYLNSTSDVSEAHNLWIDAKEQRMQEETAWPYNFVSSSFYLMARERGSISGRL 300

Query: 342 LVQDRFISSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYG 401
           LV+DRFISSSPIPARDAHVGLSAAREEG WQ ESKEYQFWV+TDSNGDFTIRN+IPGVYG
Sbjct: 301 LVRDRFISSSPIPARDAHVGLSAAREEGAWQTESKEYQFWVKTDSNGDFTIRNIIPGVYG 360

Query: 402 LHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDV 461
           LHGWVPGFIGDYLH+SLVTVSAGS+THLGILTYSPLRDGPTVWEIGFPDRTA SFYVPDV
Sbjct: 361 LHGWVPGFIGDYLHKSLVTVSAGSYTHLGILTYSPLRDGPTVWEIGFPDRTANSFYVPDV 420

Query: 462 NPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGK 521
           NPMYVNKLFLHSPEKFRQYGLW+GYSD HP NDQ+FTVGINDPKKDWF+AQVCRRGEDGK
Sbjct: 421 NPMYVNKLFLHSPEKFRQYGLWDGYSDSHPRNDQIFTVGINDPKKDWFFAQVCRRGEDGK 480

Query: 522 FVATTWSIKFNMTSLTEGTYRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTV 581
           +VATTW+IKFNM SLT+G YRLRL+IASATRSDLKINVNSM SESSLVFQLM+LGMDNTV
Sbjct: 481 YVATTWTIKFNMKSLTDGIYRLRLSIASATRSDLKINVNSMESESSLVFQLMDLGMDNTV 540

Query: 582 CRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTP 617
           CRHGNHGLYR Y+++VPSSML+KGDN+IFLTQAR GD LCG+LYDYLRLEAPD TP
Sbjct: 541 CRHGNHGLYRIYTIDVPSSMLVKGDNSIFLTQARNGDALCGILYDYLRLEAPDATP 596

BLAST of Cp4.1LG10g09680 vs. ExPASy TrEMBL
Match: A0A0A0KL71 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G513660 PE=4 SV=1)

HSP 1 Score: 1104 bits (2855), Expect = 0.0
Identity = 520/589 (88.29%), Postives = 545/589 (92.53%), Query Frame = 0

Query: 49  KSSESKRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPL 108
           +S   K  YWDINWSWPGGKDRYQLLKGSEFNVIN++DDTIEISFRK F+ STRGNKLPL
Sbjct: 45  QSIRCKIKYWDINWSWPGGKDRYQLLKGSEFNVINSSDDTIEISFRKDFDLSTRGNKLPL 104

Query: 109 SVDIRYIMRTGMPGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMP 168
            VDIRYIMRTG+PGFYCYAIYEHPS CRAFDL QTRMVFKLRQEKFHYMAISDEKQRIMP
Sbjct: 105 GVDIRYIMRTGIPGFYCYAIYEHPSECRAFDLAQTRMVFKLRQEKFHYMAISDEKQRIMP 164

Query: 169 MPEDLHPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIG 228
           MPEDL PGRGEELIVPESVLLVNPINPDLKGEVDDKYQYS DNKDGGVHGWISSSPNNIG
Sbjct: 165 MPEDLRPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSEDNKDGGVHGWISSSPNNIG 224

Query: 229 FWVVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVL 288
           FW+VFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGP+L
Sbjct: 225 FWIVFPSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPIL 284

Query: 289 VYLNSTSDVSEAHNLWIDAKEQE--------------------NERGSISGRLLVQDRFI 348
           VYLNSTSDVSEAHNLWIDAKEQ                      ERGSISGRLLV+DRF+
Sbjct: 285 VYLNSTSDVSEAHNLWIDAKEQRMQEETAWPYNFVASSFYLMARERGSISGRLLVRDRFV 344

Query: 349 SSSPIPARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPG 408
           SSSPIPARDAH+GLSAAREEG WQIESKEYQFWV+TDSNGDFTIRN+IPGVYGLHGWVPG
Sbjct: 345 SSSPIPARDAHIGLSAAREEGAWQIESKEYQFWVKTDSNGDFTIRNIIPGVYGLHGWVPG 404

Query: 409 FIGDYLHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNK 468
           FIGDYLH+SLVTVSAGS+ HLGILTYSP RDGPTVWEIGFPDRTA SFYVPDVNPMYVNK
Sbjct: 405 FIGDYLHKSLVTVSAGSYNHLGILTYSPPRDGPTVWEIGFPDRTANSFYVPDVNPMYVNK 464

Query: 469 LFLHSPEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWS 528
           LFLHSPEKFRQYGLWEGYSDLHP NDQ+FTVGINDPKKDWF+AQVCRRGEDGK+VATTW+
Sbjct: 465 LFLHSPEKFRQYGLWEGYSDLHPRNDQIFTVGINDPKKDWFFAQVCRRGEDGKYVATTWT 524

Query: 529 IKFNMTSLTEGTYRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHG 588
           IKFNMTSLT+GTYRLRL+IASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHG
Sbjct: 525 IKFNMTSLTDGTYRLRLSIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHG 584

Query: 589 LYRNYSVEVPSSMLIKGDNNIFLTQARGGDELCGLLYDYLRLEAPDDTP 617
           LYR YS+++PSSML+KGDN IFLTQAR GD LCG+LYDYLRLEAPD TP
Sbjct: 585 LYRIYSIDIPSSMLVKGDNCIFLTQARNGDPLCGILYDYLRLEAPDATP 633

BLAST of Cp4.1LG10g09680 vs. ExPASy TrEMBL
Match: A0A5D3CPD7 (Putative rhamnogalacturonate lyase B isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G006400 PE=4 SV=1)

HSP 1 Score: 1047 bits (2707), Expect = 0.0
Identity = 495/564 (87.77%), Postives = 523/564 (92.73%), Query Frame = 0

Query: 74  LKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPS 133
           LKGSEFNVIN++DDTIEISFRK F+ STRGNKLPL VDIRYIMRTG+PGFYCYAIYEHPS
Sbjct: 66  LKGSEFNVINSSDDTIEISFRKDFDLSTRGNKLPLGVDIRYIMRTGIPGFYCYAIYEHPS 125

Query: 134 ACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPI 193
            CRAFDL QTRMVFKLRQEKFHYMAISDEKQRIMPMPEDL PGRGE+LIVPESVLLVNPI
Sbjct: 126 ECRAFDLAQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLRPGRGEQLIVPESVLLVNPI 185

Query: 194 NPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTG 253
           NPDLKGEVDDKYQYS DNKDGGVHGWISSSPNNIGFW+VFPS+EFRNGGPTKQNLTVHTG
Sbjct: 186 NPDLKGEVDDKYQYSEDNKDGGVHGWISSSPNNIGFWIVFPSYEFRNGGPTKQNLTVHTG 245

Query: 254 PTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQE-- 313
           PTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGP+L+YLNSTSDVSEAHNLWIDAKEQ   
Sbjct: 246 PTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPILIYLNSTSDVSEAHNLWIDAKEQRMQ 305

Query: 314 ------------------NERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQI 373
                              ERGSISGRLLV+DRFISSSPIPARDAHVGLSAAREEG WQ 
Sbjct: 306 EETAWPYNFVSSSFYLMARERGSISGRLLVRDRFISSSPIPARDAHVGLSAAREEGAWQT 365

Query: 374 ESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILT 433
           ESKEYQFWV+TDSNGDFTIRN+IPGVYGLHGWVPGFIGDYLH+SLVTVSAGS+THLGILT
Sbjct: 366 ESKEYQFWVKTDSNGDFTIRNIIPGVYGLHGWVPGFIGDYLHKSLVTVSAGSYTHLGILT 425

Query: 434 YSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPIN 493
           YSPLRDGPTVWEIGFPDRTA SFYVPDVNPMYVNKLFLHSPEKFRQYGLW+GYSD HP N
Sbjct: 426 YSPLRDGPTVWEIGFPDRTANSFYVPDVNPMYVNKLFLHSPEKFRQYGLWDGYSDSHPRN 485

Query: 494 DQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEGTYRLRLAIASATRS 553
           DQ+FTVGINDPKKDWF+AQVCRRGEDGK+VATTW+IKFNM SLT+G YRLRL+IASATRS
Sbjct: 486 DQIFTVGINDPKKDWFFAQVCRRGEDGKYVATTWTIKFNMKSLTDGIYRLRLSIASATRS 545

Query: 554 DLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQ 613
           DLKINVNSM SESSLVFQLM+LGMDNTVCRHGNHGLYR Y+++VPSSML+KGDN+IFLTQ
Sbjct: 546 DLKINVNSMESESSLVFQLMDLGMDNTVCRHGNHGLYRIYTIDVPSSMLVKGDNSIFLTQ 605

Query: 614 ARGGDELCGLLYDYLRLEAPDDTP 617
           AR GD LCG+LYDYLRLEAPD TP
Sbjct: 606 ARNGDALCGILYDYLRLEAPDATP 629

BLAST of Cp4.1LG10g09680 vs. TAIR 10
Match: AT1G09890.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 661.4 bits (1705), Expect = 7.2e-190
Identity = 307/618 (49.68%), Postives = 418/618 (67.64%), Query Frame = 0

Query: 18  LDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLKGS 77
           +DNG+  VT+S P G V+GI+Y  +DNLL+V + E  RGYWD+ W   G    + ++KGS
Sbjct: 1   MDNGIARVTLSKPDGIVTGIEYNGIDNLLEVLNEEVNRGYWDLVWGGSGTAGGFDVIKGS 60

Query: 78  EFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSACRA 137
            F VI   ++ IE+SF + ++PS  G  +PL++D R++M +G  GFY YAIYEH     A
Sbjct: 61  NFEVIVKNEEQIELSFTRKWDPSQEGKAVPLNIDKRFVMLSGSSGFYTYAIYEHLKEWPA 120

Query: 138 FDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPINPDL 197
           F L +TR+ FKLR+EKFHYMA++D++QR MP+P+D  P RG+ L  PE+VLLVNP+    
Sbjct: 121 FSLAETRIAFKLRKEKFHYMAVTDDRQRFMPLPDDRLPDRGQALAYPEAVLLVNPLESQF 180

Query: 198 KGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPTCL 257
           KGEVDDKYQYS +NKD  VHGWI +   ++GFW++ PSHE+R GGP KQNLT H GPT L
Sbjct: 181 KGEVDDKYQYSCENKDITVHGWICTEQPSVGFWLITPSHEYRTGGPQKQNLTSHVGPTAL 240

Query: 258 AMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLN-STSDVSEAHNLWIDAKEQEN---- 317
           A+F   HY GED++    EGEAW+KVFGPV VYLN ST D ++   LW DAK Q N    
Sbjct: 241 AVFISAHYTGEDLVPKFSEGEAWKKVFGPVFVYLNSSTDDDNDPLWLWQDAKSQMNVEAE 300

Query: 318 ----------------ERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIESK 377
                           +RG++ GRLLVQDR++    I A   +VGL+     G WQ E K
Sbjct: 301 SWPYSFPASDDYVKTEQRGNVVGRLLVQDRYVDKDFIAANRGYVGLAVPGAAGSWQRECK 360

Query: 378 EYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYSP 437
           EYQFW +TD  G F I  + PG Y L+ W+PGFIGDY +  ++T+++G + ++  L Y P
Sbjct: 361 EYQFWTRTDEEGFFYISGIRPGQYNLYAWIPGFIGDYKYDDVITITSGCYIYVEDLVYQP 420

Query: 438 LRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHPINDQV 497
            R+G T+WEIGFPDR+A  FYVPD NP Y+N L+ + P++FRQYGLWE Y++L+P  D V
Sbjct: 421 PRNGATLWEIGFPDRSAAEFYVPDPNPKYINNLYQNHPDRFRQYGLWERYAELYPDKDLV 480

Query: 498 FTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTEG-TYRLRLAIASATRSDL 557
           + VG +D +KDWFYAQV R+ ++  +  TTW IKF + ++ +  +Y LR+AIASAT S+L
Sbjct: 481 YVVGSSDYRKDWFYAQVTRKKDNKTYQGTTWQIKFELKNIDKNHSYTLRVAIASATFSEL 540

Query: 558 KINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQAR 614
           +I VN+  + +S +F    +G DN++ RHG HGLY  ++VEV  S L++G+N +FLTQ R
Sbjct: 541 QIRVNN--ANASPMFTSGLIGRDNSIARHGIHGLYWLFNVEVAGSKLLEGENTLFLTQPR 600

BLAST of Cp4.1LG10g09680 vs. TAIR 10
Match: AT1G09910.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 635.2 bits (1637), Expect = 5.6e-182
Identity = 299/641 (46.65%), Postives = 425/641 (66.30%), Query Frame = 0

Query: 1   MGKKKKLRLQNQGSH-------VILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSES 60
           +  +K  R+ + G H       V++DNG+L VT+S P G ++GI+Y  +DN+L+V++ E+
Sbjct: 36  LSHRKDHRMSSHGVHLHVHDRYVVMDNGILQVTLSKPGGIITGIEYNGIDNVLEVRNKET 95

Query: 61  KRGYWDINWSWPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIR 120
            RGYWD++W+ PGGK  + ++ G  F VI  T++ +EISF + ++PS  G  +PL++D R
Sbjct: 96  NRGYWDLHWNEPGGKGIFDVISGVTFRVIVETEEQVEISFLRTWDPSLEGKYIPLNIDKR 155

Query: 121 YIMRTGMPGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDL 180
           +IM  G  G Y Y IYEH      F+L +TR+ FKLR++KFHYMA++D+++RIMP P+DL
Sbjct: 156 FIMLRGSSGVYSYGIYEHLKDWPGFELGETRIAFKLRKDKFHYMAVADDRKRIMPFPDDL 215

Query: 181 HPGRGEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVF 240
             GR + L   E+ LL  P +P L+GEVDDKYQYS +NKD  VHGWIS  P  +GFW + 
Sbjct: 216 CKGRCQTLDYQEASLLTAPCDPRLQGEVDDKYQYSCENKDLRVHGWISFDP-PVGFWQIT 275

Query: 241 PSHEFRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNS 300
           PS+EFR+GGP KQNLT H GPT LA+FH THY G+ ++   + GE W+KV+GPV +YLNS
Sbjct: 276 PSNEFRSGGPLKQNLTSHVGPTTLAVFHSTHYAGKTMMPRFEHGEPWKKVYGPVFIYLNS 335

Query: 301 TSDVSEAHNLWIDAK--------------------EQENERGSISGRLLVQDRFISSSPI 360
           T++  +   LW DAK                     +  ERG+  GRLL++DRFI++  I
Sbjct: 336 TANGDDPLCLWDDAKIKMMAEVERWPYSFVASDDYPKSEERGTARGRLLIRDRFINNDLI 395

Query: 361 PARDAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDY 420
            AR A+VGL+   + G WQIE K YQFW   D  G F+I NV PG Y L+ WVP FIGDY
Sbjct: 396 SARGAYVGLAPPGDSGSWQIECKGYQFWAIADEAGYFSIGNVRPGEYNLYAWVPSFIGDY 455

Query: 421 LHQSLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHS 480
            + ++V V++G    +G + Y P RDGPT+WEIG PDR A  F++PD +P  VN++ +H 
Sbjct: 456 HNGTIVRVTSGCMIEMGDIVYEPPRDGPTLWEIGIPDRKASEFFIPDPDPTLVNRVLVHH 515

Query: 481 PEKFRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNM 540
            ++FRQYGLW+ Y+D++P +D V+TVG++D ++DWF+A V R+  D     TTW I FN+
Sbjct: 516 QDRFRQYGLWKKYTDMYPNDDLVYTVGVSDYRRDWFFAHVPRKKGD-VHEGTTWQIIFNL 575

Query: 541 TSLTE-GTYRLRLAIASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRN 600
            ++ +   Y+LR+AIASAT ++L+I +N   +   L F    +G DN++ RHG HG+Y  
Sbjct: 576 ENIDQKANYKLRVAIASATLAELQIRINDAEAIRPL-FTTGLIGRDNSIARHGIHGVYML 635

Query: 601 YSVEVPSSMLIKGDNNIFLTQARGGDELCGLLYDYLRLEAP 614
           Y+V +P + L++GDN IFL Q R      G++YDY+RLE P
Sbjct: 636 YAVNIPGNRLVQGDNTIFLKQPRCNGPFQGIMYDYIRLEGP 673

BLAST of Cp4.1LG10g09680 vs. TAIR 10
Match: AT4G24430.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 631.3 bits (1627), Expect = 8.0e-181
Identity = 303/640 (47.34%), Postives = 428/640 (66.88%), Query Frame = 0

Query: 1   MGKKKKLRLQNQGSHVILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDI 60
           M  +  ++L  Q SHV++ NG + VTIS P G+V+GI Y  +DNLL+  + +  RGYWD+
Sbjct: 1   MSNQDSVQLDVQESHVVMGNGKVKVTISKPDGFVTGISYQGVDNLLETHNEDFNRGYWDL 60

Query: 61  NWS---WPGGKDRYQLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMR 120
            WS    PG   + + +KG+ F V+   ++ +EISF + ++ S + +  P++VD R+IMR
Sbjct: 61  VWSDEGTPGTTGKSERIKGTSFEVVVENEELVEISFSRKWDSSLQDSIAPINVDKRFIMR 120

Query: 121 TGMPGFYCYAIYEHPSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGR 180
             + GFY YAI+EH +   AF+L QTR+V+KLR++KF YMAI+D +QR MP+PED    R
Sbjct: 121 KDVTGFYSYAIFEHLAEWPAFNLPQTRIVYKLRKDKFKYMAIADNRQRKMPLPEDRLGKR 180

Query: 181 GEELIVPESVLLVNPINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHE 240
           G  L  PE+VLLV+P+  + KGEVDDKY+YS +NKD  VHGWIS +  ++G W + PS+E
Sbjct: 181 GRPLAYPEAVLLVHPVEDEFKGEVDDKYEYSSENKDLKVHGWISHN-LDLGCWQIIPSNE 240

Query: 241 FRNGGPTKQNLTVHTGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSD- 300
           FR+GG +KQNLT H GP  LAMF   HY GED++  +K G++W+KVFGPV  YLN   D 
Sbjct: 241 FRSGGLSKQNLTSHVGPISLAMFLSAHYAGEDMVMKVKAGDSWKKVFGPVFTYLNCLPDK 300

Query: 301 VSEAHNLWIDAKEQ--------------------ENERGSISGRLLVQDRFISSSPIPAR 360
            S+  +LW DAK Q                     ++RG ISGRLLV D+F+S   +PA 
Sbjct: 301 TSDPLSLWQDAKNQMLTEVQSWPYDFPASEDFPVSDKRGCISGRLLVCDKFLSDDFLPAN 360

Query: 361 DAHVGLSAAREEGGWQIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQ 420
            A VGL+   E G WQ+ESK YQFW + DS+G F I ++  G Y L+G+V G+IGDY ++
Sbjct: 361 GAFVGLAPPGEVGSWQLESKGYQFWTEADSDGYFAINDIREGEYNLNGYVTGWIGDYQYE 420

Query: 421 SLVTVSAGSHTHLGILTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEK 480
            L+ ++AG    +G + Y P RDGPTVWEIG PDR+A  F+VPD NP Y+NKL++  P++
Sbjct: 421 QLINITAGCDIDVGNIVYEPPRDGPTVWEIGIPDRSAAEFFVPDPNPKYINKLYIGHPDR 480

Query: 481 FRQYGLWEGYSDLHPINDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSL 540
           FRQYGLWE Y++L+P  D VFT+G++D KKDWF+A V R+  D  +  TTW IKF + ++
Sbjct: 481 FRQYGLWERYTELYPKEDLVFTIGVSDYKKDWFFAHVTRKMGDDTYQKTTWQIKFKLENV 540

Query: 541 TEG-TYRLRLAIASATRSDLKINVNSMGSESSL-VFQLMNLGMDNTVCRHGNHGLYRNYS 600
            +  TY++R+A+A+A  ++L++ +N   +E +  +F    +G DN + RHG HG+YR Y+
Sbjct: 541 QKSCTYKIRIALATANVAELQVRMNDDDTEKTTPIFTTGVIGHDNAIARHGIHGIYRLYN 600

Query: 601 VEVPSSMLIKGDNNIFLTQA-RGGDELCGLLYDYLRLEAP 614
           V+VPS  L++GDN +FLTQ         GL+YDY+RLE P
Sbjct: 601 VDVPSEKLVEGDNTLFLTQTMTTTGAFNGLMYDYIRLEGP 639

BLAST of Cp4.1LG10g09680 vs. TAIR 10
Match: AT1G09880.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 610.5 bits (1573), Expect = 1.5e-174
Identity = 295/627 (47.05%), Postives = 417/627 (66.51%), Query Frame = 0

Query: 18  LDNGLLMVTISNPQGYVSGIKYGNMDNLLD-VKSSESKRGYWDINWSWPGGKDR-----Y 77
           ++N  L +T+SNP+G+V+GI+Y  +DN+L    + E  RGYWD+ W++PG K +      
Sbjct: 1   MENRFLQLTLSNPEGFVTGIQYNGIDNVLAYYTNKEYDRGYWDVVWNFPGKKAKKTKGTL 60

Query: 78  QLLKGSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEH 137
             ++ ++  VI   D+ IE+SF + +N S+    +P+++D R++M     GFY YAI+E 
Sbjct: 61  DRIEATKMEVITQNDEKIELSFTRTWNTSST-TAVPVNIDKRFVMLQNSSGFYSYAIFER 120

Query: 138 PSACRAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVN 197
                A +L   R+VFKL ++KFHYMAISD++QR MP+P+D  P RG+ L  PE+V L++
Sbjct: 121 LQGWPAVELDNMRLVFKLNKKKFHYMAISDDRQRYMPVPDDRVPPRGQPLAYPEAVQLLD 180

Query: 198 PINPDLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVH 257
           PI P+ KGEVDDKY+YS+++KD  VHGWIS++ +++GFW + PS+EFR+ GP KQ L  H
Sbjct: 181 PIEPEFKGEVDDKYEYSMESKDIKVHGWISTN-DSVGFWQITPSNEFRSAGPLKQFLGSH 240

Query: 258 TGPTCLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQ- 317
            GPT LA+FH THY+G D++   K GEAW+KVFGPV +YLNS     +   LW +AK Q 
Sbjct: 241 VGPTNLAVFHSTHYVGADLIMSFKNGEAWKKVFGPVFIYLNSFPKGVDPLLLWHEAKNQT 300

Query: 318 -------------------ENERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGW 377
                               ++RGS+SGRLLV+DRFISS  IPA  ++VGL+A  + G W
Sbjct: 301 KIEEEKWPYNFTASDDFPASDQRGSVSGRLLVRDRFISSEDIPANGSYVGLAAPGDVGSW 360

Query: 378 QIESKEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGI 437
           Q E K YQFW + D NG F+I NV  G Y L+ + PGFIGDY + ++  +S GS   LG 
Sbjct: 361 QRECKGYQFWSKADENGSFSINNVRSGRYNLYAFAPGFIGDYHNDTVFDISPGSKISLGD 420

Query: 438 LTYSPLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHSPEKFRQYGLWEGYSDLHP 497
           L Y P RDG T+WEIG PDR+A  FY+PD NP +VNKL+L+  +K+RQYGLWE YS+L+P
Sbjct: 421 LVYEPPRDGSTLWEIGVPDRSAAEFYIPDPNPSFVNKLYLNHSDKYRQYGLWERYSELYP 480

Query: 498 INDQVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKF----NMTSLTEGTYRLRLAI 557
             D V+ V I+D  K+WF+ QV R+  +G +  TTW I+F     M ++T G ++LR+A+
Sbjct: 481 DEDMVYNVDIDDYSKNWFFMQVTRKQANGGYKGTTWQIRFQFDDKMKNVT-GNFKLRIAL 540

Query: 558 ASATRSDLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDN 615
           A++  ++L++ VN + ++  L F+   +G DNT+ RHG HGLY  YSV VP++ L  G+N
Sbjct: 541 ATSNVAELQVRVNDLSADPPL-FRTEQIGRDNTIARHGIHGLYWLYSVNVPAASLHVGNN 600

BLAST of Cp4.1LG10g09680 vs. TAIR 10
Match: AT2G22620.1 (Rhamnogalacturonate lyase family protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.9e-170
Identity = 289/620 (46.61%), Postives = 396/620 (63.87%), Query Frame = 0

Query: 16  VILDNGLLMVTISNPQGYVSGIKYGNMDNLLDVKSSESKRGYWDINWSWPGGKDRYQLLK 75
           V++DNG++ VT SNP+G ++GIKY  +DN+LD K  +  RGYWD+ W  P  K +   L+
Sbjct: 62  VVVDNGIVQVTFSNPEGLITGIKYHGIDNVLDDKIDD--RGYWDVVWYEPEKKQKTDKLE 121

Query: 76  GSEFNVINATDDTIEISFRKVFNPSTRGNKLPLSVDIRYIMRTGMPGFYCYAIYEHPSAC 135
           G++F +I   ++ IEISF + +  S RG+ +PL+VD RYI+R+G+ G Y Y I E     
Sbjct: 122 GTKFEIITQNEEQIEISFTRTWTISRRGSLVPLNVDKRYIIRSGVSGLYMYGILERLEGW 181

Query: 136 RAFDLVQTRMVFKLRQEKFHYMAISDEKQRIMPMPEDLHPGRGEELIVPESVLLVNPINP 195
              D+ Q R+VFKL  +KF +MAISD++QR MP   D      + L   E+VLL NP NP
Sbjct: 182 PDVDMDQIRIVFKLNPKKFDFMAISDDRQRSMPSMADRE--NSKSLAYKEAVLLTNPSNP 241

Query: 196 DLKGEVDDKYQYSVDNKDGGVHGWISSSPNNIGFWVVFPSHEFRNGGPTKQNLTVHTGPT 255
             KGEVDDKY YS+++KD  VHGWISS P  +GFW++ PS EFR GGP KQ+LT H GP 
Sbjct: 242 MFKGEVDDKYMYSMEDKDNNVHGWISSDP-PVGFWMITPSDEFRLGGPIKQDLTSHAGPI 301

Query: 256 CLAMFHGTHYIGEDILTHIKEGEAWRKVFGPVLVYLNSTSDVSEAHNLWIDAKEQ----- 315
            L+MF  THY G+++    + GE W+KVFGPVL YLNS S       LW DAK Q     
Sbjct: 302 TLSMFTSTHYAGKEMRMDYRNGEPWKKVFGPVLAYLNSVSPKDSTLRLWRDAKRQMAAEV 361

Query: 316 ---------------ENERGSISGRLLVQDRFISSSPIPARDAHVGLSAAREEGGWQIES 375
                           ++RG++ G+ L++D ++S   I  + A VGL+   E G WQ ES
Sbjct: 362 KSWPYDFITSEDYPLRHQRGTLEGQFLIKDSYVSRLKIYGKFAFVGLAPIGEAGSWQTES 421

Query: 376 KEYQFWVQTDSNGDFTIRNVIPGVYGLHGWVPGFIGDYLHQSLVTVSAGSHTHLGILTYS 435
           K YQFW + D  G F I NV  G Y L+ W  GFIGDY ++  +T++ GS  ++G L Y 
Sbjct: 422 KGYQFWTKADRRGRFIIENVRAGNYSLYAWGSGFIGDYKYEQNITITPGSEMNVGPLVYE 481

Query: 436 PLRDGPTVWEIGFPDRTAGSFYVPDVNPMYVNKLFLHS-PEKFRQYGLWEGYSDLHPIND 495
           P R+GPT+WEIG PDRTAG FY+PD  P  +NKL+++   ++FRQYGLW+ Y+DL+P ND
Sbjct: 482 PPRNGPTLWEIGVPDRTAGEFYIPDPYPTLMNKLYVNPLQDRFRQYGLWDRYADLYPQND 541

Query: 496 QVFTVGINDPKKDWFYAQVCRRGEDGKFVATTWSIKFNMTSLTE-GTYRLRLAIASATRS 555
            V+T+G++D + DWF+A V R   +  +  TTW I FN+ ++   G Y LR+A+ASA  S
Sbjct: 542 LVYTIGVSDYRSDWFFAHVARNVGNDTYQPTTWQIIFNLKNVNRIGRYTLRIALASAADS 601

Query: 556 DLKINVNSMGSESSLVFQLMNLGMDNTVCRHGNHGLYRNYSVEVPSSMLIKGDNNIFLTQ 614
           +L+I +N    +S  +F    +G DN + RHG HGLYR YS++V  ++L  GDN IFLTQ
Sbjct: 602 ELQIRIND--PKSDAIFTTGFIGKDNAIARHGIHGLYRLYSIDVAGNLLSVGDNTIFLTQ 661