Cp4.1LG10g08290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG10g08290
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Proteasome inhibitor PI31 subunit
Location	Cp4.1LG10: 5132419 .. 5135524 (+)
RNA-Seq Expression	Cp4.1LG10g08290
Synteny	Cp4.1LG10g08290

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAAAGTCATTTTGATGACAGCACTAGTAGACGCTCCTTGCATAGAGAGCCACCAAATCTTGCTCTCTCTCTCTGGGTCTTGCTCCGTCGGAATTGAATTGGTGAAGAACCAAGAATCTACAAAGCAATACAAAAATGGTGAATGAGCAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACCTTTCGCAATGACAACGACAAGGTCGCCTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGGTATCACTTCTTTCTTGTTTATTGAAAAATTAAAAATTCTTCCCATATTTAGTATTTCTGAGTCTTGGATTGGGGTTTAGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAATCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCGCTCCATCTTGAAATTGAGTACGCTTTCCCAGATATCATATATATATATATGTGTATTGGCAAATTAGTACGTTGTCCTGTTTCGGTATTGAGTGTTTTTTTGTTCCTATTGTTCTTTTAGCGTTGAAGACATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACTCTGGTGAAACGCTTGGGCTCGGGAATATTCTCAAAATTGGGTGGTTATTCGAAAGCCACGACAAGTAATCCAGCAAGGTAGTGATTAGATCAGTAATCAACACGTACGGTTATTTTAATTCTTCATTTTTCCTGATGTTATTGAGACGATTACTCTCTTTTGTGATTCTTATTTCTTGGCGTGTTATAAATATGAATCGAAATTATGTCTCTGGTAATCGTTCTTTAATCTGGTTCTTGGCGGTCTACATGCTTTTTGCAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCTACTTCTGGAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGGTCTTTCTCTTTATCCACACTTGCGCAACCACAAAATGTCATGACATAATTTGAGAAATATGTTGGGTCTTGACTTGAGTTCTTAACTATTGTATGCTACCATGATTTTACGTTTGTGTTGGGTGCTATTATCACTGTCTCCAACATTCAGATTTGCTAGTTTTTAAGTTTCTAACAGTAATTTGAATGCATTTTCTTTATGATATCTTGTTTTCCCCTTTATGGTTAGATCAAGAAGATAATGGTTTATATGCTTTGTGCTTTGATTGATTTGATACCCAGTTCCTCTTAGTGGTTTATAGGCTTTGATTGATTTGACACAAGAAGATAATGGTTTCCTTTGAGAGAGATTGTAATAGTCTTACTTAACGTGCAGGAATTGTTTTTGCTGAATTATTGTGACGGTCAAATATTTAATGGCAAGCATTTTGTGCCTTCGTCCAATCCTTAATTGTATCAATTTAGGAATTTTCGAAGGTTCCATGTTTACAAATAATTTATCTATGAACCTGTATCCCATGAGTGAGTGAGTGAGTTGACATAGTCTGAATGTGGCTGTTTTTGCTTTTCTCTTAGAGTTGTAATTCCTCCTATTAATCCCTTTGGTGGTGGCGATCTTTTTCCTGGACCTGGTGCTGGAATGTACCCTGCTGGGTAATTTATTTAAGTTTGTCGATCATTTGTGTACGTATGTGTAACAGTCCAAGCCTACCGCTAGTCGATATTGTTTTTTTTTTGCATTCCCTCAAGGTTTTTAAAATGTGTTTGTGAGAGGTCTCCACAATCTTAAAAAGAATGTTTGTTCCCCTCTCCAATCGCTGTGGGATCTCACAATCCAATCCCCTTCGAGGCTCAGCATTCTCGTTGGCACTTTATACTCTCTCTAATTGATGTGGGATCTCACATCGATTGAAGAGGGGAATGAAACATTTTTTATAAAGGGGTGGAAACTTCTCCCTAGCAGACGTGTTTTAAAAACCTTGAGGGAAAACTCGAAAAGAAAAATACAACGAGAACAATATTTGCCAATGATAGACTTTGACTATCATAGTACGATTGTCATCGGCGCAAAATATCATGTTAATTTTGTTTTTATGTTTTTTTTTTCCAATTGATTACAGAGGTGGTTTAAGCGGCGATGGGAGCATGCTTTTAGGTTGGTGAATTGTTAAGTCTTCTAGTACTAGATTGAAAATAAGCGTAATTTCACTATTTTATCATGCAGGACCTAATGACCCGCGATGGTTAGGAGTTGGTAGAGGACCTGGATTTGAAGGCGGGCAGCCGTGAGTCAATTAGCTCTCTCTCTCACTCATATAGACACACACAAACACTCACCTTTGCATTATGACTGCTGCTAATTCCAAGTATCTTGAAACAGGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGTTAGGTATATGGAATTTTCCTTACCGTGCCATAGCATATTGGGGCGGGGGCTCTCTCTTTGAATCATTAATTTCTATCAGCGTTTATCATTTGCTGAATGCCTGCTTTGACAACGACTAAATTCAAACCATGTATCTTGGCATGCTAAATTAGGATATGTTCATAGAAACAACTTGTTTCTGAATACATGTTGTATGGAGCGGCAGAGTAATGAATTAACTGGGATGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCCTTTGTTACAGAAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAGCATTTTGGAGACGGTTCAGATTTCATATAGTCCGCCATAGTTATAAAGCTCTGAAAGTTGTTGAAAGTTACTTTTTTCTTCTGTTTTAGGTGGCCAGTAATGACTGTACGTACGGTGAATATAGGAATGGTGTTTACTGAATTTTACACGTGTCAAAAAACATTTTAACTTCAAACTTTTACACCCATATAAATAATAATGTATGTATTTTACCTATTATAT

mRNA sequence

CGAAAGTCATTTTGATGACAGCACTAGTAGACGCTCCTTGCATAGAGAGCCACCAAATCTTGCTCTCTCTCTCTGGGTCTTGCTCCGTCGGAATTGAATTGGTGAAGAACCAAGAATCTACAAAGCAATACAAAAATGGTGAATGAGCAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACCTTTCGCAATGACAACGACAAGGTCGCCTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAATCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCGCTCCATCTTGAAATTGACGTTGAAGACATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACTCTGGTGAAACGCTTGGGCTCGGGAATATTCTCAAAATTGGGTGGTTATTCGAAAGCCACGACAAGTAATCCAGCAAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCTACTTCTGGAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGAGTTGTAATTCCTCCTATTAATCCCTTTGGTGGTGGCGATCTTTTTCCTGGACCTGGACCTAATGACCCGCGATGGTTAGGAGTTGGTAGAGGACCTGGATTTGAAGGCGGGCAGCCGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGTTAGAAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAGCATTTTGGAGACGGTTCAGATTTCATATAGTCCGCCATAGTTATAAAGCTCTGAAAGTTGTTGAAAGTTACTTTTTTCTTCTGTTTTAGGTGGCCAGTAATGACTGTACGTACGGTGAATATAGGAATGGTGTTTACTGAATTTTACACGTGTCAAAAAACATTTTAACTTCAAACTTTTACACCCATATAAATAATAATGTATGTATTTTACCTATTATAT

Coding sequence (CDS)

ATGGTGAATGAGCAATCGGTTATGGCCGTCATCAGGGCAACTAGGCCTACCTTTCGCAATGACAACGACAAGGTCGCCTTTGCGGTTCACGCCATATTTCTCGCCTCCGGCTGCGTGCTCACTGCCACCGGACCTCCTGCTTTTACTGACGCCGCCTTTTCTTCTCCCTCAAAGGATGAAATTAGTATCGAGGGCTGGAACGAATTAGAGGATGAGTACGCATACGTTTACGCAAATCTTGAAAAGGATTCTAACTGGAAGAAAGTGATAGTGAAGTGCCTGGTGATGAACGGGAAATTGCTTATTGATGCCTTGACTGATGAGAATTCCGAGCCGCTCCATCTTGAAATTGACGTTGAAGACATCGTCAATCCGAATGGGGGAAGTAACTATTCAACGCAGTACAGAAATTTGGATACTCTGGTGAAACGCTTGGGCTCGGGAATATTCTCAAAATTGGGTGGTTATTCGAAAGCCACGACAAGTAATCCAGCAAGGGCTGAGAGTGAGAGTAGTGAAAGATCATCTGTAGTTGTAAATAAGCCTACTTCTGGAATTCCTGAGCTTCCTGGTCCACTGCTCGATCCTTCCGGAGTTGTAATTCCTCCTATTAATCCCTTTGGTGGTGGCGATCTTTTTCCTGGACCTGGACCTAATGACCCGCGATGGTTAGGAGTTGGTAGAGGACCTGGATTTGAAGGCGGGCAGCCGGGTGTTCCTCCAGGTGCTCGTTTTGATCCATATGGTCCACCTGGTGTTCCTGGTTTTGAACCCTTTCGGTTTGTTAGAAATCCTGGGAGACCTGGAGGGGGAACTCATCCGGACCTGGAGCATTTTGGAGACGGTTCAGATTTCATATAG

Protein sequence

MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVNKPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPGPNDPRWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI

Homology

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy Swiss-Prot
Match: Q9M330 (Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 5.6e-73
Identity = 164/310 (52.90%), Postives = 197/310 (63.55%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           M N Q+VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           D    +   +YS Q++NLD LV  L S I  KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGG-GDLFPGP------------------GPNDP 240
            P        GP + PSGVV+PPI   GG  DLFPGP                  GP DP
Sbjct: 181 TPNP-----LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGFGDGSMLVGPTDP 240

Query: 241 RWLGVGRG---PGFEG-GQPGV-PPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDL 287
           R+   G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF R P R  GG HPDL
Sbjct: 241 RFFPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFTRQPPRGPGGGHPDL 300

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy Swiss-Prot
Match: Q92530 (Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2)

HSP 1 Score: 54.7 bits (130), Expect = 2.0e-06
Identity = 61/217 (28.11%), Postives = 98/217 (45.16%), Query Frame = 0


Query: 58  KDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEI 117
           K E+   GWN  +D Y   Y   E     +K++VK + +   ++++ L   + +   L +
Sbjct: 50  KSELLPAGWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTL 109

Query: 118 DVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKL-GGYSKATTSNPARAESESSERSS 177
           +++D ++     ++   Y+N + L  R+ SGI + +   + KA  S+P R    ++ R  
Sbjct: 110 NLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREV 169

Query: 178 VVVNKP-----TSGIPELPGPLLDPSGVVI---PPINPFG---GGDLFPGPGPNDPRWLG 237
             +  P     TS  P    P  DP G  +     ++PFG   GG +        PR L 
Sbjct: 170 DPLRIPPHHPHTSRQP----PWCDPLGPFVVGGEDLDPFGPRRGGMIVDPLRSGFPRAL- 229

Query: 238 VGRGPGFEGGQP--GVPPGARFDPYGPPGV--PGFEP 259
           +    G     P   VPPGARFDP+GP G   PG  P
Sbjct: 230 IDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNP 258

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy Swiss-Prot
Match: Q5RDN3 (Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.0e-06
Identity = 61/214 (28.50%), Postives = 97/214 (45.33%), Query Frame = 0


Query: 58  KDEISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEI 117
           K E+   GWN  +D Y   Y   E     +K++VK + +   ++++ L   + +   L +
Sbjct: 50  KSELLPAGWNNNKDLYVLRY---EYKDGSRKLLVKAITVESSMILNVLEYGSQQVADLTL 109

Query: 118 DVEDIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKL-GGYSKATTSNPARAESESSERSS 177
           +++D ++     ++   Y+N + L  R+ SGI + +   + KA  S+P R    ++ R  
Sbjct: 110 NLDDYIDAEHLGDFHRTYKNSEELRSRIVSGIITPIHEQWEKANVSSPHREFPPATAREV 169

Query: 178 VVVNKP-----TSGIPELPGPLLDPSGVVIPPINPFG---GGDLFPGPGPNDPRWLGVGR 237
             +  P     TS  P    P L P  V    ++PFG   GG +        PR L +  
Sbjct: 170 DPLRIPPHHPHTSRQPPWCDP-LGPFAVGGEDLDPFGHRRGGMIVDPLRSGFPRAL-IDP 229

Query: 238 GPGFEGGQP--GVPPGARFDPYGPPGV--PGFEP 259
             G     P   VPPGARFDP+GP G   PG  P
Sbjct: 230 SSGLPNRLPPGAVPPGARFDPFGPIGTSPPGPNP 258

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy Swiss-Prot
Match: Q8BHL8 (Proteasome inhibitor PI31 subunit OS=Mus musculus OX=10090 GN=Psmf1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.3e-05
Identity = 64/248 (25.81%), Postives = 109/248 (43.95%), Query Frame = 0


Query: 16  PTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDEISIEGWNELEDEYAY 75
           P+     D +   +H   + +G     TG     D    S  K E+    WN  ++ YA 
Sbjct: 13  PSMSCPQDALVCFLHWEVVTNGYYALGTG-----DQPGPSDKKSELLPAKWNSNKELYAL 72

Query: 76  VYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVEDIVNPNGGSNYSTQY 135
            Y   E     +K+++K + +   ++I+ L     +   L ++++D ++    S++   Y
Sbjct: 73  RY---ESKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTY 132

Query: 136 RNLDTLVKRLGSGIFSKL-GGYSKATTSNPAR----AESESSERSSVVVNKP-TSGIPEL 195
           +N + L  ++ SGI + +   + KA  ++P R    A +   +   +  ++P TS  P  
Sbjct: 133 KNSEELRSQIRSGIITPIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAW 192

Query: 196 PGPLLDPSGVVIPPINPFG---GGDLFPGPGPNDPRWLGVGRGPGFEGGQP--GVPPGAR 253
             P L P  V    ++PFG   GG +        PR L +    G     P   VPPGAR
Sbjct: 193 RDP-LSPFAVGGDDLDPFGCQRGGMIVDPLRSGFPRVL-IDPSSGLPNRLPPGAVPPGAR 250

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy Swiss-Prot
Match: Q5XIU5 (Proteasome inhibitor PI31 subunit OS=Rattus norvegicus OX=10116 GN=Psmf1 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 6.5e-05
Identity = 64/248 (25.81%), Postives = 106/248 (42.74%), Query Frame = 0


Query: 16  PTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDEISIEGWNELEDEYAY 75
           P      D +   +H   + +G     TG     D    +  K E+    WN  ++ YA 
Sbjct: 13  PAMTCPQDALVCFLHWEVVTNGYYGLGTG-----DQPDPNDKKSELLPAEWNSNKELYAL 72

Query: 76  VYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVEDIVNPNGGSNYSTQY 135
            Y   E     +K+++K + +   ++I+ L     +   L ++++D ++    S++   Y
Sbjct: 73  RY---ESKDGARKLLLKAVSVENGMIINVLEHGTQQVADLTLNLDDYIDAEDLSDFHRTY 132

Query: 136 RNLDTLVKRLGSGIFSKL-GGYSKATTSNPAR----AESESSERSSVVVNKP-TSGIPEL 195
           +N + L  R+ SGI + +   + K   S+P R    A +   +   +  + P TS  P  
Sbjct: 133 KNSEELRSRIRSGIITPIHEQWEKVRLSSPPREFPPATAREVDPLRISSHHPHTSRQPTW 192

Query: 196 PGPLLDPSGVVIPPINPFG---GGDLFPGPGPNDPRWLGVGRGPGFEGGQP--GVPPGAR 253
             P L P  V    ++PFG   GG +        PR L +    G     P   VPPGAR
Sbjct: 193 RDP-LSPFAVGGEDLDPFGCQRGGMIVDPLRSGFPRAL-IDPSSGLPNRLPPGAVPPGAR 250

[Close]

BLAST of Cp4.1LG10g08290 vs. NCBI nr
Match: XP_023545137.1 (probable proteasome inhibitor isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567 bits (1461), Expect = 1.90e-203
Identity = 286/305 (93.77%), Postives = 286/305 (93.77%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN
Sbjct: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG-------------------PNDP 240
           KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG                   PNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPGAGMYPAGGGLSGDGSMLLGPNDP 240

Query: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 286
           RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 300

[Close]

BLAST of Cp4.1LG10g08290 vs. NCBI nr
Match: XP_022950229.1 (probable proteasome inhibitor isoform X1 [Cucurbita moschata])

HSP 1 Score: 553 bits (1425), Expect = 9.79e-198
Identity = 279/305 (91.48%), Postives = 280/305 (91.80%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 75  ISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG-------------------PNDP 240
           KPTSGIPELPGPLLDPSGVVIPPINP GGGDLFPGPG                   PNDP
Sbjct: 195 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAGMYPTGGGFGGDGSMLLGPNDP 254

Query: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 286
           RWLG GRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGRPGGGTHPDLEHFGD
Sbjct: 255 RWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGRPGGGTHPDLEHFGD 314

[Close]

BLAST of Cp4.1LG10g08290 vs. NCBI nr
Match: XP_022978238.1 (probable proteasome inhibitor isoform X1 [Cucurbita maxima])

HSP 1 Score: 550 bits (1417), Expect = 9.69e-197
Identity = 278/305 (91.15%), Postives = 281/305 (92.13%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           +IVNPNGGSNYSTQYRNLDTLVKRLG GI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG-------------------PNDP 240
           KPTSGIPELPGPLLDPSGVVIPPINP GGGDLFPGPG                   PNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 240

Query: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 286
           RWLG+G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 300

[Close]

BLAST of Cp4.1LG10g08290 vs. NCBI nr
Match: XP_023545138.1 (probable proteasome inhibitor isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 532 bits (1370), Expect = 5.42e-190
Identity = 270/286 (94.41%), Postives = 272/286 (95.10%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN
Sbjct: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPGPNDPRWLGVGRGPGFEGGQPGVP 240
           KPTSGIPELPGPLLDPSG  +      G G +  GP  NDPRWLGVGRGPGFEGGQPGVP
Sbjct: 181 KPTSGIPELPGPLLDPSGGGL-----SGDGSMLLGP--NDPRWLGVGRGPGFEGGQPGVP 240

Query: 241 PGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI 286
           PGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI
Sbjct: 241 PGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI 279

[Close]

BLAST of Cp4.1LG10g08290 vs. NCBI nr
Match: XP_022950230.1 (probable proteasome inhibitor isoform X2 [Cucurbita moschata])

HSP 1 Score: 521 bits (1341), Expect = 2.38e-185
Identity = 266/287 (92.68%), Postives = 268/287 (93.38%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 75  ISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGG-GDLFPGPGPNDPRWLGVGRGPGFEGGQPGV 240
           KPTSGIPELPGPLLDPSG        FGG G +  GP  NDPRWLG GRGPGFEGGQPGV
Sbjct: 195 KPTSGIPELPGPLLDPSG------GGFGGDGSMLLGP--NDPRWLGTGRGPGFEGGQPGV 254

Query: 241 PPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI 286
           PPGARFDPYGPPGVPGFEPFRF RNPGRPGGGTHPDLEHFGDGSDFI
Sbjct: 255 PPGARFDPYGPPGVPGFEPFRFARNPGRPGGGTHPDLEHFGDGSDFI 293

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy TrEMBL
Match: A0A6J1GF71 (probable proteasome inhibitor isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 553 bits (1425), Expect = 4.74e-198
Identity = 279/305 (91.48%), Postives = 280/305 (91.80%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 75  ISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG-------------------PNDP 240
           KPTSGIPELPGPLLDPSGVVIPPINP GGGDLFPGPG                   PNDP
Sbjct: 195 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAGMYPTGGGFGGDGSMLLGPNDP 254

Query: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 286
           RWLG GRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGRPGGGTHPDLEHFGD
Sbjct: 255 RWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGRPGGGTHPDLEHFGD 314

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy TrEMBL
Match: A0A6J1ISG6 (probable proteasome inhibitor isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478282 PE=4 SV=1)

HSP 1 Score: 550 bits (1417), Expect = 4.69e-197
Identity = 278/305 (91.15%), Postives = 281/305 (92.13%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           +IVNPNGGSNYSTQYRNLDTLVKRLG GI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG-------------------PNDP 240
           KPTSGIPELPGPLLDPSGVVIPPINP GGGDLFPGPG                   PNDP
Sbjct: 181 KPTSGIPELPGPLLDPSGVVIPPINPLGGGDLFPGPGAEMYPAGGGFSGDGSMLLGPNDP 240

Query: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 286
           RWLG+G GPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD
Sbjct: 241 RWLGIGIGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 300

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy TrEMBL
Match: A0A6J1GF52 (probable proteasome inhibitor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 521 bits (1341), Expect = 1.15e-185
Identity = 266/287 (92.68%), Postives = 268/287 (93.38%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 75  ISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPARAESESSERSSVVVN 194

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGG-GDLFPGPGPNDPRWLGVGRGPGFEGGQPGV 240
           KPTSGIPELPGPLLDPSG        FGG G +  GP  NDPRWLG GRGPGFEGGQPGV
Sbjct: 195 KPTSGIPELPGPLLDPSG------GGFGGDGSMLLGP--NDPRWLGTGRGPGFEGGQPGV 254

Query: 241 PPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI 286
           PPGARFDPYGPPGVPGFEPFRF RNPGRPGGGTHPDLEHFGDGSDFI
Sbjct: 255 PPGARFDPYGPPGVPGFEPFRFARNPGRPGGGTHPDLEHFGDGSDFI 293

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy TrEMBL
Match: A0A6J1ITK3 (probable proteasome inhibitor isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478282 PE=4 SV=1)

HSP 1 Score: 516 bits (1330), Expect = 3.28e-184
Identity = 263/286 (91.96%), Postives = 267/286 (93.36%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNE+SVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 1   MVNEKSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           +IVNPNGGSNYSTQYRNLDTLVKRLG GI SKLGG SKATTSNPARAESESSERSSVVVN
Sbjct: 121 EIVNPNGGSNYSTQYRNLDTLVKRLGLGILSKLGGSSKATTSNPARAESESSERSSVVVN 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPGPNDPRWLGVGRGPGFEGGQPGVP 240
           KPTSGIPELPGPLLDPSG         G G +  GP  NDPRWLG+G GPGFEGGQPGVP
Sbjct: 181 KPTSGIPELPGPLLDPSGGGF-----SGDGSMLLGP--NDPRWLGIGIGPGFEGGQPGVP 240

Query: 241 PGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI 286
           PGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI
Sbjct: 241 PGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGDGSDFI 279

[Close]

BLAST of Cp4.1LG10g08290 vs. ExPASy TrEMBL
Match: A0A6J1GE95 (probable proteasome inhibitor isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111453381 PE=4 SV=1)

HSP 1 Score: 479 bits (1233), Expect = 2.64e-169
Identity = 247/305 (80.98%), Postives = 248/305 (81.31%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE
Sbjct: 15  MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 74

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           ISIEGWNELEDEYAYVYAN EKD+NWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE
Sbjct: 75  ISIEGWNELEDEYAYVYANFEKDANWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 134

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           DIVNPNGGSNYSTQYRNLDTLVKRLGSGI SKLGG SKATTSNPAR              
Sbjct: 135 DIVNPNGGSNYSTQYRNLDTLVKRLGSGILSKLGGSSKATTSNPAR-------------- 194

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGGGDLFPGPG-------------------PNDP 240
                             VVIPPINP GGGDLFPGPG                   PNDP
Sbjct: 195 ------------------VVIPPINPLGGGDLFPGPGAGMYPTGGGFGGDGSMLLGPNDP 254

Query: 241 RWLGVGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDLEHFGD 286
           RWLG GRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRF RNPGRPGGGTHPDLEHFGD
Sbjct: 255 RWLGTGRGPGFEGGQPGVPPGARFDPYGPPGVPGFEPFRFARNPGRPGGGTHPDLEHFGD 287

[Close]

BLAST of Cp4.1LG10g08290 vs. TAIR 10
Match: AT3G53970.1 (proteasome inhibitor-related )

HSP 1 Score: 275.8 bits (704), Expect = 3.9e-74
Identity = 164/310 (52.90%), Postives = 197/310 (63.55%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           M N Q+VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           D    +   +YS Q++NLD LV  L S I  KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGG-GDLFPGP------------------GPNDP 240
            P        GP + PSGVV+PPI   GG  DLFPGP                  GP DP
Sbjct: 181 TPNP-----LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAGMYPGRGGFGDGSMLVGPTDP 240

Query: 241 RWLGVGRG---PGFEG-GQPGV-PPGARFDPYGPPGVPGFEPFRFVRNPGRPGGGTHPDL 287
           R+   G G   PGF G   PG+ PPGARFDPYGPPGVPGFEP RF R P R  GG HPDL
Sbjct: 241 RFFPFGDGSDRPGFMGPPHPGMPPPGARFDPYGPPGVPGFEPGRFTRQPPRGPGGGHPDL 300

[Close]

BLAST of Cp4.1LG10g08290 vs. TAIR 10
Match: AT3G53970.2 (proteasome inhibitor-related )

HSP 1 Score: 201.8 bits (512), Expect = 7.2e-52
Identity = 126/258 (48.84%), Postives = 160/258 (62.02%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFTDAAFSSPSKDE 60
           M N Q+VMA+IR  RP FRN++DKVAFA+H+ F+ASG +LTATG PAF D A SS S+++
Sbjct: 1   MANSQTVMAMIRLARPPFRNNHDKVAFAIHSSFVASGYILTATGRPAFADEALSSSSQND 60

Query: 61  ISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDVE 120
           + IEGWNE E EYA+VYAN +K S  KK++VKCL M+ KLL+DA+ D  +EP HLEI V 
Sbjct: 61  VGIEGWNEFEGEYAFVYANPKKGS--KKILVKCLAMDDKLLVDAIADGGAEPAHLEIKVG 120

Query: 121 DIVNPNGGSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSNPARAESESSERSSVVVN 180
           D    +   +YS Q++NLD LV  L S I  KL G  K   S  A++ SE++E      +
Sbjct: 121 DYAEESNEGDYSAQFKNLDKLVTDLQSEIIDKLDGKPKPVASR-AQSSSETNEEPRYYDD 180

Query: 181 KPTSGIPELPGPLLDPSGVVIPPINPFGG-GDLFPGPGPNDPRWLGVGRGPGFEGGQPGV 240
            P        GP + PSGVV+PPI   GG  DLFPGPG      +  GRG   +G     
Sbjct: 181 TPNP-----LGPQIHPSGVVVPPIPGNGGYSDLFPGPGAG----MYPGRGGFGDGSMLVG 240

Query: 241 PPGARFDPYGP-PGVPGF 257
           P   RF P+G     PGF
Sbjct: 241 PTDPRFFPFGDGSDRPGF 246

[Close]

BLAST of Cp4.1LG10g08290 vs. TAIR 10
Match: AT1G48530.1 (CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST Arabidopsis thaliana protein match is: proteasome inhibitor-related (TAIR:AT3G53970.2); Has 43 Blast hits to 43 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 11; Fungi - 0; Plants - 32; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 134.0 bits (336), Expect = 1.9e-31
Identity = 75/165 (45.45%), Postives = 102/165 (61.82%), Query Frame = 0


Query: 1   MVNEQSVMAVIRATRPTFRNDNDKVAFAVHAIFLASGCVLTATGPPAFT-DAAFSSPSKD 60
           M    +VM VIR+  P FRN NDK+AF VHA    SG +LT+TG PAF  DA  SS ++ 
Sbjct: 12  MATTHAVMGVIRSVMPAFRNKNDKIAFVVHASLAVSGFILTSTGRPAFAHDALSSSTTQC 71

Query: 61  EISIEGWNELEDEYAYVYANLEKDSNWKKVIVKCLVMNGKLLIDALTDENSEPLHLEIDV 120
            + IEGWNE ++EYA+VY         K+ +VKCL MN KLL+DA+ ++  E  HL+I+V
Sbjct: 72  LVGIEGWNEFDEEYAFVY-----KCPVKRYLVKCLAMNDKLLVDAIAEDGKEFGHLQIEV 131

Query: 121 EDIVNPNG-GSNYSTQYRNLDTLVKRLGSGIFSKLGGYSKATTSN 164
            + ++ +G   +Y TQ++N D LV  L S I  KL   S  T S+
Sbjct: 132 GNYIDESGEEGDYDTQFKNFDKLVTELKSEILCKLNTVSTTTKSS 171

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g08290 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M330	5.6e-73	52.90	Probable proteasome inhibitor OS=Arabidopsis thaliana OX=3702 GN=At3g53970 PE=1 ...	[more]
Q92530	2.0e-06	28.11	Proteasome inhibitor PI31 subunit OS=Homo sapiens OX=9606 GN=PSMF1 PE=1 SV=2	[more]
Q5RDN3	2.0e-06	28.50	Proteasome inhibitor PI31 subunit OS=Pongo abelii OX=9601 GN=PSMF1 PE=2 SV=1	[more]
Q8BHL8	1.3e-05	25.81	Proteasome inhibitor PI31 subunit OS=Mus musculus OX=10090 GN=Psmf1 PE=1 SV=1	[more]
Q5XIU5	6.5e-05	25.81	Proteasome inhibitor PI31 subunit OS=Rattus norvegicus OX=10116 GN=Psmf1 PE=1 SV...	[more]

BLAST of Cp4.1LG10g08290 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023545137.1	1.90e-203	93.77	probable proteasome inhibitor isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022950229.1	9.79e-198	91.48	probable proteasome inhibitor isoform X1 [Cucurbita moschata]	[more]
XP_022978238.1	9.69e-197	91.15	probable proteasome inhibitor isoform X1 [Cucurbita maxima]	[more]
XP_023545138.1	5.42e-190	94.41	probable proteasome inhibitor isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022950230.1	2.38e-185	92.68	probable proteasome inhibitor isoform X2 [Cucurbita moschata]	[more]

BLAST of Cp4.1LG10g08290 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GF71	4.74e-198	91.48	probable proteasome inhibitor isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ISG6	4.69e-197	91.15	probable proteasome inhibitor isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1GF52	1.15e-185	92.68	probable proteasome inhibitor isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ITK3	3.28e-184	91.96	probable proteasome inhibitor isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A6J1GE95	2.64e-169	80.98	probable proteasome inhibitor isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]

BLAST of Cp4.1LG10g08290 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G53970.1	3.9e-74	52.90	proteasome inhibitor-related	[more]
AT3G53970.2	7.2e-52	48.84	proteasome inhibitor-related	[more]
AT1G48530.1	1.9e-31	45.45	CONTAINS InterPro DOMAIN/s: Proteasome Inhibitor PI31 (InterPro:IPR021625); BEST...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021625	PI31 proteasome regulator, N-terminal	PFAM	PF11566	PI31_Prot_N	coord: 15..153 e-value: 1.1E-14 score: 54.4
None	No IPR available	GENE3D	3.40.1000.30		coord: 2..168 e-value: 5.2E-40 score: 138.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 237..286
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 158..182
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 158..194
IPR045128	Proteasome inhibitor PI31-like	PANTHER	PTHR13266	PROTEASOME INHIBITOR	coord: 1..282

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG10g08290.1	Cp4.1LG10g08290.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
biological_process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0070628	proteasome binding