Cp4.1LG10g07330 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g07330
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionE3 ubiquitin-protein ligase Praja-2
LocationCp4.1LG10: 5888192 .. 5891911 (+)
RNA-Seq ExpressionCp4.1LG10g07330
SyntenyCp4.1LG10g07330
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TATCCATTAAAAGGAGCAACCAGGGCCGGGCGCAGTGGTGGATAGGCTGAGCCATGGGAGGCTCTGAGTGAGGCCCATAACATTCGAAGAGCGGCGAATCAATCGGACCCATTTCAAGCGCTTTATCTAGAAATCCAACTTTTTCTAGGCGCAAAGAAACCAATTAGATTGAAGTTTGATCAACATTCAGATACACGAATGAATCATTGAATTCCACTCTCTAATTTTTGTTGATTTCTTTTTCTTCTTTGTTCGAGGAATGTTGGTGGGTCAATGTCAATCACACCGCGCGTTTTGATTTTGGAAGTTCTCGATTTCCATCCTCCAATGGCGGCCGCGACTCCTACAAATCGCTTTTCACGAAGAACTTCGTTGACCTCCGCCTGAAAATCCCCATTGTTTTCCGTTTTATCCTCTGATTTCTGTTCGTCTTTGTACCATTTTAACCGACACACGACGGATGATTCTGAACAGCGTTCTTCAATTATTTGCCTCAATCGCTTTGCTCTTTTCGCGGATTGTCTTATTTTCTTCGTTTTCTTCGATTGGATGAGCTTGAGCCGAGTGTTGGAGTTCTGAAGGACTGAGAGCAAACTCACTGGTAATAATCACATCCCATCTTCGTCTTTTCTGATATCTTTTATGCATTTTTTTTCGTTTGCCCTAATTGTTTGTAGTGCTGTTTTTGTGTCTCTGAGAATATGATTTGGTTTCTGATTTGGGGGTTTTGGTCTCTGGCTTGCTAATTTGGTATCGGAGGGAGTACCATTGTTGTATGATTCGTTGAATCCTTGATATCTATGTTAGCAATTGTTGAAGCTTTTAGTATCAAAATTGGGGATGGAACTTGTGGCTGATATTAGTTCTTTATTAACTCCCATCATGGTTTGAGAAGATTCTTCCCTGGTTCACTATATCTTTCATGCTTTAGGAGTTCTTACAGTTCAAGTGTTCATCAATGGAGGTTGATTGGACTATTCCATGCTATTGATTAAAGGGCAGTTCAGTTTAGATTGAGATAAGAATATGGTTTAGTTTGAAATGAAGAGAGTGAAGTGAGGTTGGGAATGAAGGGGTGATGATGTTATGTAATACTGCATGTCTGATGTCGTTCTCTCTTTTGATCTTTTCCCATCTAGAGTTTGGAGGGTGGGGAGTAGTTGTCAGTGTACTAGAGGAAACACGGACAAGGAACGGCTCATGACATGTAATAGCCCAAGCTCACCGCTAGGAGATATTGTCTTCTTTGGGTTTTCTGTTTTGGGCTCTTCTCAAGGTTTTAAAACGCATCTACTAGAGAGAGTTGTCCACACCCTTAGAAGGAATGCTTCATTCCCCTCTGTAACTGATGTGGGATCTCACAATCCATCCCCCTTGGGGGGCTAACGTCCTCGTTGGCTCACTGCTCGGTGTCTGGGTCTGATACCATTTGTAACATCCCAATCCCACTGCTAGTAGATATTGTTCTCTTGGGTTCTCCCTTATGGGCTTTCCTTCAAGGTTTTTAAACGTGTCTACTAAGGAGAGGTTTTCACACCCTTAGAAGGAATGTTTCGTCCCCCTCTCTAATCGATGTGGGATCTCACAATCTACCTCCCTTGGGGGGCCTAGCATCCTTGCTGGCACGTTACCCAGTGTGTGATTCTGATACCATTTGTAACAGCCCAAGTCCACCGCTAGTAGATATTGTCCTCTTTGGGCTTTCCCTTCTGGGCTTCCCCTCAAGGTTTTAAAACGTGTTTGCTAGGGAGAGGGTTCCACACCCTTACAAGGAATGTTTTGTTCTCCTCTCCAACCGATGTGGGATCTCACATGACAGTAATCTGATTTGGTCTCACTTTTACGCTATTGTTTCATCACTTCTTGAACATCATATTGGTTTCCTAACGTTCGTTATTTGTGCTCGCTAGTCTCGTTTTAGATGGACATTGACTCACAAAGAATCTTGGAGGAGTTTCCTCAATCCACTGCTCACATCATCATGGACAATAATCAACAAGTCAACGAACTTGGTCCGGTTCAGCCGCTAACTTGTGCGTTCTGCCGAAGTGTCCTAGAACCTGAAAACGATGCCACTGCTGAATCTGAAGCCATTGGTATATGTGGCGATTGTAAATTTTTGTTATTCGAAGATATTGAAACCCCTTTACGGGATTCGTCTCACAGGACAACGCCAAGAGGAAGAAGAACAAGGCATGGTAGTTCGGAGTCTGCTGAAAATCTTTTTTCACAGGAATTTACCCAGATGATTAACTTGGTGAGGCAAAGACAATCCAGCATCTCTGGTCACGGTGATCGTTATACCGATGGTTCCAATGCTGCTGATTTCATGCAGCACTCGAGTTCCCATACGACCCCTAATCAATCTAGAAGATGGCGGCAAGTATATTCCGACTCTGAAAATGATGGTTTAGATAGTTTGGACTCTATGTTTGGGGAAAGTGAATCAAATTTCAGTTTTGGACCTTACAGGCATTCTTATGGTGATAGTGATGTTGTTTCCTATAGAACTTATGGGGGTGACTCAGATGCTTCCCTGGATGGACATAGTTTCTTGGATACAGACAATTTTGTTCTTCCAGATGAGGGATACGATGTCGATACCGATACAGACATTGATCCGATGCGTGCTGGTGTCGTCCATTGGGACTCGAGCGATGAGGAGGATGATCGTGGTGAATTAACAGAAGCTGACACAGAGCATGATGCACTTCAAGATTTTCATGTTTCTAGTGCTACTGGGAGAGGAAACTATGGTAACTGGAATGAAGAACTTTCTTCGTCCGAATACGAGGGTTCGGCTCGTGATTCAATTACCAACAATGCACGGTCGGAGTTGGGGGATTCTGGTGACTACCTCGACAGACAGGGTTCGTCTCCTGGGCAAATCAGGAGGAACATGCGATTTTATTCGATTACCAACTACGCACAATCGGAATCAGTATCTTACGTTGGGGATTCTGGTGACTATCTTGACAGACAAAGCTTTGAAGAACTGCTCGAGCAAATTGCCGAGACGACCAGCTCAAGACGTGGAGCGCCTCCTGCAGCCATCTCTTTTGTGAAAAATCTCTCACGTTTGGTGATTTCTAAAGAACATTTGAAGCATGACAGTATATCTTGTGCAATTTGCAAGGATTTCTTTCTTGTAGGGGTTGAAGTGAATCAACTGCCTTGTCTTCATCTCTACCACCCTCCCTGCATCTTGCCATGGTTGACTGCACGGAATTCGTGCCCCCTTTGTCGATACGAGCTTCCAACCGATGATCGTGATTACGAAGAGGGAAAGCAAAGTAGTACCAATGCTGCAGAGATCCAACGACTTCAGCATCAAGTAACTAGACTCGAAAGTCGCAGCGACTTGAACGGAGTTGGAGCAAATCCAGAGTTCGATACCAGCCAAGGAGAGGAGCTGAGCAACATAGACCCTGCAGTTAGTAATAGTTACAATTCAGGCAGAGGTCGACGAAGTTGGCTATTCGTCGCAGCTCCGGTAGTTGGTCTCGTAGGCATTGCCCTGATGTTTTGGGTCGGCAATCCTTCTGGAGGGCGAATTACAGACAGAAGGCAGCTTGATACACGCGGTGCAGATTCATCAAACCAAAGAGAGAAGAGAACTAGGAGATGGTGGTAAGTCGTCTAGCAATCGATCGCCAGGTTCGGTATGAAAAACCGAACTAATGCGAGTTGTTTTGTTAATTTCCCAGGATTGAAATCTAG

mRNA sequence

TATCCATTAAAAGGAGCAACCAGGGCCGGGCGCAGTGGTGGATAGGCTGAGCCATGGGAGGCTCTGAGTGAGGCCCATAACATTCGAAGAGCGGCGAATCAATCGGACCCATTTCAAGCGCTTTATCTAGAAATCCAACTTTTTCTAGGCGCAAAGAAACCAATTAGATTGAAGTTTGATCAACATTCAGATACACGAATGAATCATTGAATTCCACTCTCTAATTTTTGTTGATTTCTTTTTCTTCTTTGTTCGAGGAATGTTGGTGGGTCAATGTCAATCACACCGCGCGTTTTGATTTTGGAAGTTCTCGATTTCCATCCTCCAATGGCGGCCGCGACTCCTACAAATCGCTTTTCACGAAGAACTTCGTTGACCTCCGCCTGAAAATCCCCATTGTTTTCCGTTTTATCCTCTGATTTCTGTTCGTCTTTGTACCATTTTAACCGACACACGACGGATGATTCTGAACAGCGTTCTTCAATTATTTGCCTCAATCGCTTTGCTCTTTTCGCGGATTGTCTTATTTTCTTCGTTTTCTTCGATTGGATGAGCTTGAGCCGAGTGTTGGAGTTCTGAAGGACTGAGAGCAAACTCACTGTCTCGTTTTAGATGGACATTGACTCACAAAGAATCTTGGAGGAGTTTCCTCAATCCACTGCTCACATCATCATGGACAATAATCAACAAGTCAACGAACTTGGTCCGGTTCAGCCGCTAACTTGTGCGTTCTGCCGAAGTGTCCTAGAACCTGAAAACGATGCCACTGCTGAATCTGAAGCCATTGGTATATGTGGCGATTGTAAATTTTTGTTATTCGAAGATATTGAAACCCCTTTACGGGATTCGTCTCACAGGACAACGCCAAGAGGAAGAAGAACAAGGCATGGTAGTTCGGAGTCTGCTGAAAATCTTTTTTCACAGGAATTTACCCAGATGATTAACTTGGTGAGGCAAAGACAATCCAGCATCTCTGGTCACGGTGATCGTTATACCGATGGTTCCAATGCTGCTGATTTCATGCAGCACTCGAGTTCCCATACGACCCCTAATCAATCTAGAAGATGGCGGCAAGTATATTCCGACTCTGAAAATGATGGTTTAGATAGTTTGGACTCTATGTTTGGGGAAAGTGAATCAAATTTCAGTTTTGGACCTTACAGGCATTCTTATGGTGATAGTGATGTTGTTTCCTATAGAACTTATGGGGGTGACTCAGATGCTTCCCTGGATGGACATAGTTTCTTGGATACAGACAATTTTGTTCTTCCAGATGAGGGATACGATGTCGATACCGATACAGACATTGATCCGATGCGTGCTGGTGTCGTCCATTGGGACTCGAGCGATGAGGAGGATGATCGTGGTGAATTAACAGAAGCTGACACAGAGCATGATGCACTTCAAGATTTTCATGTTTCTAGTGCTACTGGGAGAGGAAACTATGGTAACTGGAATGAAGAACTTTCTTCGTCCGAATACGAGGGTTCGGCTCGTGATTCAATTACCAACAATGCACGGTCGGAGTTGGGGGATTCTGGTGACTACCTCGACAGACAGGGTTCGTCTCCTGGGCAAATCAGGAGGAACATGCGATTTTATTCGATTACCAACTACGCACAATCGGAATCAGTATCTTACGTTGGGGATTCTGGTGACTATCTTGACAGACAAAGCTTTGAAGAACTGCTCGAGCAAATTGCCGAGACGACCAGCTCAAGACGTGGAGCGCCTCCTGCAGCCATCTCTTTTGTGAAAAATCTCTCACGTTTGGTGATTTCTAAAGAACATTTGAAGCATGACAGTATATCTTGTGCAATTTGCAAGGATTTCTTTCTTGTAGGGGTTGAAGTGAATCAACTGCCTTGTCTTCATCTCTACCACCCTCCCTGCATCTTGCCATGGTTGACTGCACGGAATTCGTGCCCCCTTTGTCGATACGAGCTTCCAACCGATGATCGTGATTACGAAGAGGGAAAGCAAAGTAGTACCAATGCTGCAGAGATCCAACGACTTCAGCATCAAGTAACTAGACTCGAAAGTCGCAGCGACTTGAACGGAGTTGGAGCAAATCCAGAGTTCGATACCAGCCAAGGAGAGGAGCTGAGCAACATAGACCCTGCAGTTAGTAATAGTTACAATTCAGGCAGAGGTCGACGAAGTTGGCTATTCGTCGCAGCTCCGGTAGTTGGTCTCGTAGGCATTGCCCTGATGTTTTGGGTCGGCAATCCTTCTGGAGGGCGAATTACAGACAGAAGGCAGCTTGATACACGCGGTGCAGATTCATCAAACCAAAGAGAGAAGAGAACTAGGAGATGGTGGATTGAAATCTAG

Coding sequence (CDS)

ATGGACATTGACTCACAAAGAATCTTGGAGGAGTTTCCTCAATCCACTGCTCACATCATCATGGACAATAATCAACAAGTCAACGAACTTGGTCCGGTTCAGCCGCTAACTTGTGCGTTCTGCCGAAGTGTCCTAGAACCTGAAAACGATGCCACTGCTGAATCTGAAGCCATTGGTATATGTGGCGATTGTAAATTTTTGTTATTCGAAGATATTGAAACCCCTTTACGGGATTCGTCTCACAGGACAACGCCAAGAGGAAGAAGAACAAGGCATGGTAGTTCGGAGTCTGCTGAAAATCTTTTTTCACAGGAATTTACCCAGATGATTAACTTGGTGAGGCAAAGACAATCCAGCATCTCTGGTCACGGTGATCGTTATACCGATGGTTCCAATGCTGCTGATTTCATGCAGCACTCGAGTTCCCATACGACCCCTAATCAATCTAGAAGATGGCGGCAAGTATATTCCGACTCTGAAAATGATGGTTTAGATAGTTTGGACTCTATGTTTGGGGAAAGTGAATCAAATTTCAGTTTTGGACCTTACAGGCATTCTTATGGTGATAGTGATGTTGTTTCCTATAGAACTTATGGGGGTGACTCAGATGCTTCCCTGGATGGACATAGTTTCTTGGATACAGACAATTTTGTTCTTCCAGATGAGGGATACGATGTCGATACCGATACAGACATTGATCCGATGCGTGCTGGTGTCGTCCATTGGGACTCGAGCGATGAGGAGGATGATCGTGGTGAATTAACAGAAGCTGACACAGAGCATGATGCACTTCAAGATTTTCATGTTTCTAGTGCTACTGGGAGAGGAAACTATGGTAACTGGAATGAAGAACTTTCTTCGTCCGAATACGAGGGTTCGGCTCGTGATTCAATTACCAACAATGCACGGTCGGAGTTGGGGGATTCTGGTGACTACCTCGACAGACAGGGTTCGTCTCCTGGGCAAATCAGGAGGAACATGCGATTTTATTCGATTACCAACTACGCACAATCGGAATCAGTATCTTACGTTGGGGATTCTGGTGACTATCTTGACAGACAAAGCTTTGAAGAACTGCTCGAGCAAATTGCCGAGACGACCAGCTCAAGACGTGGAGCGCCTCCTGCAGCCATCTCTTTTGTGAAAAATCTCTCACGTTTGGTGATTTCTAAAGAACATTTGAAGCATGACAGTATATCTTGTGCAATTTGCAAGGATTTCTTTCTTGTAGGGGTTGAAGTGAATCAACTGCCTTGTCTTCATCTCTACCACCCTCCCTGCATCTTGCCATGGTTGACTGCACGGAATTCGTGCCCCCTTTGTCGATACGAGCTTCCAACCGATGATCGTGATTACGAAGAGGGAAAGCAAAGTAGTACCAATGCTGCAGAGATCCAACGACTTCAGCATCAAGTAACTAGACTCGAAAGTCGCAGCGACTTGAACGGAGTTGGAGCAAATCCAGAGTTCGATACCAGCCAAGGAGAGGAGCTGAGCAACATAGACCCTGCAGTTAGTAATAGTTACAATTCAGGCAGAGGTCGACGAAGTTGGCTATTCGTCGCAGCTCCGGTAGTTGGTCTCGTAGGCATTGCCCTGATGTTTTGGGTCGGCAATCCTTCTGGAGGGCGAATTACAGACAGAAGGCAGCTTGATACACGCGGTGCAGATTCATCAAACCAAAGAGAGAAGAGAACTAGGAGATGGTGGATTGAAATCTAG

Protein sequence

MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGICGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSISGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSFGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVVHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNPSGGRITDRRQLDTRGADSSNQREKRTRRWWIEI
Homology
BLAST of Cp4.1LG10g07330 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 9.2e-27
Identity = 56/137 (40.88%), Postives = 81/137 (59.12%), Query Frame = 0

Query: 330 YSITNYAQSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVI 389
           + I N+         G+ GDY      E+L++Q+AE   +R G PPA+ S +  L  + +
Sbjct: 145 FVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKV 204

Query: 390 SKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDD 449
           +K+ LK +   CA+C D F  G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD
Sbjct: 205 TKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD 264

Query: 450 RDYEEGKQSSTNAAEIQ 467
            DYE   Q S  + + Q
Sbjct: 265 PDYENRSQGSQGSGDGQ 281

BLAST of Cp4.1LG10g07330 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.3e-25
Identity = 59/143 (41.26%), Postives = 82/143 (57.34%), Query Frame = 0

Query: 311 DYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELLEQIAETTSSR 370
           ++L+   SS  QI      + I N    +      + GDY      E+L++Q+AE   +R
Sbjct: 139 NHLNDLRSSGAQIE-----FVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNR 198

Query: 371 RGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILP 430
            G PPA+ S ++ L  + I+K +L  +   CA+C D F  G E  Q+PC HLYH  C+LP
Sbjct: 199 YGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLP 258

Query: 431 WLTARNSCPLCRYELPTDDRDYE 454
           WL   NSCP+CR+ELPTDD DYE
Sbjct: 259 WLELHNSCPVCRHELPTDDPDYE 276

BLAST of Cp4.1LG10g07330 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 3.1e-22
Identity = 53/129 (41.09%), Postives = 73/129 (56.59%), Query Frame = 0

Query: 345 GDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAIC 404
           G++GDY      EEL EQ++  T +RRG PPA  S +  L  + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 405 KDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELP------------TDDRDY 462
           KD F +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

BLAST of Cp4.1LG10g07330 vs. ExPASy Swiss-Prot
Match: Q9SPL2 (E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.0e-22
Identity = 55/128 (42.97%), Postives = 77/128 (60.16%), Query Frame = 0

Query: 339 ESVSYVGDSGDYL-DRQSFEELLEQIAETT----SSRRGAPPAAISFVKNLSRLVISKEH 398
           ES  Y G+  DY+ D   +E LL+ +AE        RRGAPPAA S ++ L    +S   
Sbjct: 191 ESDRYAGNPADYIDDAAGYEALLQNLAEGDGGGGGGRRGAPPAAKSAIEALETFEVSSSE 250

Query: 399 LKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYE 458
            +   + CA+CKD  ++G    +LPC H YH  CI+PWL  RNSCP+CR++L TDD +YE
Sbjct: 251 GEMVMV-CAVCKDGMVMGETGKKLPCGHCYHGDCIVPWLGTRNSCPVCRFQLETDDAEYE 310

Query: 459 EGKQSSTN 462
           E ++  T+
Sbjct: 311 EERKKRTS 317

BLAST of Cp4.1LG10g07330 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 7.6e-21
Identity = 52/132 (39.39%), Postives = 74/132 (56.06%), Query Frame = 0

Query: 346 DSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICK 405
           ++GDY      EEL+EQ++  T   RG PPA  S +  L  + I+++HLK     C +CK
Sbjct: 132 NAGDYFFGPGLEELIEQLSSGT-HHRGPPPAPKSSIDALPTIKITQKHLKSSDSHCPVCK 191

Query: 406 DFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPT--DDRDYEEGKQSSTNAA 465
           D F +  E  Q+PC H+YH  CI+PWL   NSCP+CR ELP+       +  +  STN  
Sbjct: 192 DEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGSSSSTQSSQNRSTNGR 251

Query: 466 EIQRLQHQVTRL 476
           E  R ++  + L
Sbjct: 252 ENSRRRNIFSNL 262

BLAST of Cp4.1LG10g07330 vs. NCBI nr
Match: XP_023543725.1 (E3 ubiquitin-protein ligase Praja-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1149 bits (2971), Expect = 0.0
Identity = 570/572 (99.65%), Postives = 572/572 (100.00%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF
Sbjct: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300
           HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300

Query: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360
           NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL
Sbjct: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360

Query: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420
           EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL
Sbjct: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420

Query: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480
           HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD
Sbjct: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480

Query: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540
           LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP
Sbjct: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540

Query: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWWIE 572
           SGGRITDRRQLDTRGADSSNQREKRTRRWW++
Sbjct: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWWLK 572

BLAST of Cp4.1LG10g07330 vs. NCBI nr
Match: KAG7033695.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1126 bits (2912), Expect = 0.0
Identity = 559/570 (98.07%), Postives = 562/570 (98.60%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTC FCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCVFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           SGHGDRYTDGSNAADFMQHSSSH TPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF
Sbjct: 121 SGHGDRYTDGSNAADFMQHSSSHMTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNF+LPDEG  VDTDTDIDPMRAGVV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFILPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300
           HWDSSDEEDDRGELTEADTEHDALQDFHVSSA+GRGNYGNWNEELS SEYEGSARDSITN
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSASGRGNYGNWNEELSLSEYEGSARDSITN 300

Query: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360
           NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL
Sbjct: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360

Query: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420
           EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHD ISCAICKDFFLVGVEVNQLPCL
Sbjct: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDGISCAICKDFFLVGVEVNQLPCL 420

Query: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480
           HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD
Sbjct: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480

Query: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540
           LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP
Sbjct: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540

Query: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           SGGRITDRRQLDTR ADSSNQRE RTRRWW
Sbjct: 541 SGGRITDRRQLDTRSADSSNQRENRTRRWW 570

BLAST of Cp4.1LG10g07330 vs. NCBI nr
Match: KAG6603514.1 (E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 559/570 (98.07%), Postives = 562/570 (98.60%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTC FCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCVFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF
Sbjct: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNF+LPDEG  VDTDTDIDPMRAGVV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFILPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300
           HWDSSDEEDDRGELTEADTEHDALQDFHVSSA+GRGNYGNWNEELS SEYEGSARDSITN
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSASGRGNYGNWNEELSLSEYEGSARDSITN 300

Query: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360
           NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL
Sbjct: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360

Query: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420
           EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHD ISCAICKDFFLVGVEVNQLPCL
Sbjct: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDGISCAICKDFFLVGVEVNQLPCL 420

Query: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480
           HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD
Sbjct: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480

Query: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540
           LNGVGANPE DTSQG ELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP
Sbjct: 481 LNGVGANPELDTSQGVELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540

Query: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           SGGRITDRRQLDTRGADSSNQRE RTRRWW
Sbjct: 541 SGGRITDRRQLDTRGADSSNQRENRTRRWW 570

BLAST of Cp4.1LG10g07330 vs. NCBI nr
Match: XP_022950070.1 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 560/570 (98.25%), Postives = 562/570 (98.60%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEFP STA IIMDNNQQVNELGPVQPLTCAFCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           SGH DRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEG  VDTDTDIDPMRAGVV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300
           HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300

Query: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360
           NA+SELGDSGDYLDRQGSSPGQIRRNMRFYSITNYA SESVSYVGDSGDYLDRQSFEELL
Sbjct: 301 NAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELL 360

Query: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420
           EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL
Sbjct: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420

Query: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480
           HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD
Sbjct: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480

Query: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540
           LN VGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP
Sbjct: 481 LNEVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540

Query: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           SGGRITDRRQLDTRGADSSNQRE RTRRWW
Sbjct: 541 SGGRITDRRQLDTRGADSSNQRENRTRRWW 570

BLAST of Cp4.1LG10g07330 vs. NCBI nr
Match: XP_022977372.1 (E3 ubiquitin-protein ligase Praja-2 [Cucurbita maxima])

HSP 1 Score: 1104 bits (2856), Expect = 0.0
Identity = 555/582 (95.36%), Postives = 564/582 (96.91%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEF QSTAHIIMD+NQQVNELGPVQPLTCAFCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFSQSTAHIIMDSNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLV-RQRQSS 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSS SAENLFSQEFTQMINLV RQRQSS
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSVSAENLFSQEFTQMINLVVRQRQSS 120

Query: 121 ISGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFS 180
           ISGHGDRYT+GS+AADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFS
Sbjct: 121 ISGHGDRYTNGSSAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFS 180

Query: 181 FGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGV 240
           FGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPD G DVDTDTDIDPMRAGV
Sbjct: 181 FGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDGGSDVDTDTDIDPMRAGV 240

Query: 241 VHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARD--- 300
           VHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSAR+   
Sbjct: 241 VHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSAREQVR 300

Query: 301 ------SITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDY 360
                 SITNNA+SELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDY
Sbjct: 301 RNRQFYSITNNAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDY 360

Query: 361 LDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLV 420
           LDRQSFEELLEQIAETT SRRGAPPAAISFVKNLSRLVISKEHLKHD ISCAICKDFFLV
Sbjct: 361 LDRQSFEELLEQIAETTISRRGAPPAAISFVKNLSRLVISKEHLKHDIISCAICKDFFLV 420

Query: 421 GVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQH 480
           GVEVNQLPCLHLYHPPCILPWL+ARNSCPLCRYELPTDD DYEEGKQSSTNAAEIQRLQH
Sbjct: 421 GVEVNQLPCLHLYHPPCILPWLSARNSCPLCRYELPTDDHDYEEGKQSSTNAAEIQRLQH 480

Query: 481 QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVG 540
           QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVG
Sbjct: 481 QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVG 540

Query: 541 IALMFWVGNPSGGRITDRRQLDTRGADSSNQREKRTRRWWIE 572
           IALMFWVGNPSGGRITDRRQLDTRGADSSNQRE RTRRWW++
Sbjct: 541 IALMFWVGNPSGGRITDRRQLDTRGADSSNQRENRTRRWWLK 582

BLAST of Cp4.1LG10g07330 vs. ExPASy TrEMBL
Match: A0A6J1GEL6 (E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 1123 bits (2906), Expect = 0.0
Identity = 560/570 (98.25%), Postives = 562/570 (98.60%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEFP STA IIMDNNQQVNELGPVQPLTCAFCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           SGH DRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEG  VDTDTDIDPMRAGVV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300
           HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300

Query: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360
           NA+SELGDSGDYLDRQGSSPGQIRRNMRFYSITNYA SESVSYVGDSGDYLDRQSFEELL
Sbjct: 301 NAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELL 360

Query: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420
           EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL
Sbjct: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420

Query: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480
           HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD
Sbjct: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480

Query: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540
           LN VGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP
Sbjct: 481 LNEVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540

Query: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           SGGRITDRRQLDTRGADSSNQRE RTRRWW
Sbjct: 541 SGGRITDRRQLDTRGADSSNQRENRTRRWW 570

BLAST of Cp4.1LG10g07330 vs. ExPASy TrEMBL
Match: A0A6J1II95 (E3 ubiquitin-protein ligase Praja-2 OS=Cucurbita maxima OX=3661 GN=LOC111477725 PE=4 SV=1)

HSP 1 Score: 1104 bits (2856), Expect = 0.0
Identity = 555/582 (95.36%), Postives = 564/582 (96.91%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEF QSTAHIIMD+NQQVNELGPVQPLTCAFCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFSQSTAHIIMDSNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLV-RQRQSS 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSS SAENLFSQEFTQMINLV RQRQSS
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSVSAENLFSQEFTQMINLVVRQRQSS 120

Query: 121 ISGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFS 180
           ISGHGDRYT+GS+AADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFS
Sbjct: 121 ISGHGDRYTNGSSAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFS 180

Query: 181 FGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGV 240
           FGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPD G DVDTDTDIDPMRAGV
Sbjct: 181 FGPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDGGSDVDTDTDIDPMRAGV 240

Query: 241 VHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARD--- 300
           VHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSAR+   
Sbjct: 241 VHWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSAREQVR 300

Query: 301 ------SITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDY 360
                 SITNNA+SELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDY
Sbjct: 301 RNRQFYSITNNAQSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDY 360

Query: 361 LDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLV 420
           LDRQSFEELLEQIAETT SRRGAPPAAISFVKNLSRLVISKEHLKHD ISCAICKDFFLV
Sbjct: 361 LDRQSFEELLEQIAETTISRRGAPPAAISFVKNLSRLVISKEHLKHDIISCAICKDFFLV 420

Query: 421 GVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQH 480
           GVEVNQLPCLHLYHPPCILPWL+ARNSCPLCRYELPTDD DYEEGKQSSTNAAEIQRLQH
Sbjct: 421 GVEVNQLPCLHLYHPPCILPWLSARNSCPLCRYELPTDDHDYEEGKQSSTNAAEIQRLQH 480

Query: 481 QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVG 540
           QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVG
Sbjct: 481 QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVG 540

Query: 541 IALMFWVGNPSGGRITDRRQLDTRGADSSNQREKRTRRWWIE 572
           IALMFWVGNPSGGRITDRRQLDTRGADSSNQRE RTRRWW++
Sbjct: 541 IALMFWVGNPSGGRITDRRQLDTRGADSSNQRENRTRRWWLK 582

BLAST of Cp4.1LG10g07330 vs. ExPASy TrEMBL
Match: A0A6J1GDT0 (uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453262 PE=4 SV=1)

HSP 1 Score: 1062 bits (2746), Expect = 0.0
Identity = 535/570 (93.86%), Postives = 537/570 (94.21%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDIDSQRILEEFP STA IIMDNNQQVNELGPVQPLTCAFCRSVLEP+NDATAESEAIGI
Sbjct: 1   MDIDSQRILEEFPHSTARIIMDNNQQVNELGPVQPLTCAFCRSVLEPDNDATAESEAIGI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI
Sbjct: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           SGH DRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF
Sbjct: 121 SGHDDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEG  VDTDTDIDPMRAGVV
Sbjct: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGSGVDTDTDIDPMRAGVV 240

Query: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARDSITN 300
           HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSAR+    
Sbjct: 241 HWDSSDEEDDRGELTEADTEHDALQDFHVSSATGRGNYGNWNEELSSSEYEGSARE---- 300

Query: 301 NARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLDRQSFEELL 360
                                QIRRNMRFYSITNYA SESVSYVGDSGDYLDRQSFEELL
Sbjct: 301 ---------------------QIRRNMRFYSITNYAHSESVSYVGDSGDYLDRQSFEELL 360

Query: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420
           EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL
Sbjct: 361 EQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGVEVNQLPCL 420

Query: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480
           HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD
Sbjct: 421 HLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQVTRLESRSD 480

Query: 481 LNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540
           LN VGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP
Sbjct: 481 LNEVGANPEFDTSQGEELSNIDPAVSNSYNSGRGRRSWLFVAAPVVGLVGIALMFWVGNP 540

Query: 541 SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           SGGRITDRRQLDTRGADSSNQRE RTRRWW
Sbjct: 541 SGGRITDRRQLDTRGADSSNQRENRTRRWW 545

BLAST of Cp4.1LG10g07330 vs. ExPASy TrEMBL
Match: A0A0A0L2Z4 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 PE=4 SV=1)

HSP 1 Score: 833 bits (2151), Expect = 9.52e-300
Identity = 436/587 (74.28%), Postives = 470/587 (80.07%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDID QRILEEFPQS  H+IM+NNQ+V+E  P+Q  TCAFC+ VL P++DA +E E I I
Sbjct: 1   MDIDLQRILEEFPQSNVHVIMNNNQRVDEHSPIQLPTCAFCQRVLVPDSDAPSEPEPIDI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRR    SSES ENLFSQEFT MIN+VRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRAMRSSSESPENLFSQEFTHMINMVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           S HGDRY DGSNA DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMF ESESNFSF
Sbjct: 121 SSHGDRYADGSNAMDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFEESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLD-GHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGV 240
           GPYRHSYGDSDVVS RTYGG+SD SLD GHSFLDTD F+LP EG D  TDTDIDPMRAGV
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGESDVSLDDGHSFLDTDVFILPGEGSD--TDTDIDPMRAGV 240

Query: 241 VHWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYE 300
            HWDSSDEEDD GE TEA+TE +         LQ+FHVSSA+ RGN  NWNE+LSS EYE
Sbjct: 241 GHWDSSDEEDDHGEFTEAETEQEVGSSEARPQLQNFHVSSASRRGNSSNWNEQLSSPEYE 300

Query: 301 GSARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYL 360
                                    GS+PG IRRN RFYSITNYAQSE +SYVGDSGDYL
Sbjct: 301 -------------------------GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYL 360

Query: 361 DRQSFEELLEQIAETT-SSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLV 420
           DRQ FEELLEQIAETT SSRRGAPPAA+SFVKNL RLVISKEHLKHDSISCAICKDF  +
Sbjct: 361 DRQGFEELLEQIAETTTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLHL 420

Query: 421 GVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQH 480
           GVEVNQLPCLHLYHP CILPWL+ARNSCPLCRYELPTDDRDYEE KQSS NAA +  LQ 
Sbjct: 421 GVEVNQLPCLHLYHPSCILPWLSARNSCPLCRYELPTDDRDYEEVKQSSINAAAVHGLQP 480

Query: 481 QVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRG-RRSWLFVAAPVVGLV 540
           +V  LE+ S LNGVGANPEF+TSQ E+  NID AV +S NSGRG RR WLFVAAP+VGLV
Sbjct: 481 RVVGLENPSGLNGVGANPEFETSQEEDQRNIDSAVISSNNSGRGARRRWLFVAAPIVGLV 540

Query: 541 GIALMFWVGNP------SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           GIALM W GNP      SGG++TD RQLD   A+SSNQRE R+RRWW
Sbjct: 541 GIALMLWFGNPLCDQRVSGGQLTDGRQLDAHSAESSNQRENRSRRWW 560

BLAST of Cp4.1LG10g07330 vs. ExPASy TrEMBL
Match: A0A5D3C795 (E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold287G00690 PE=4 SV=1)

HSP 1 Score: 828 bits (2138), Expect = 6.98e-298
Identity = 433/585 (74.02%), Postives = 468/585 (80.00%), Query Frame = 0

Query: 1   MDIDSQRILEEFPQSTAHIIMDNNQQVNELGPVQPLTCAFCRSVLEPENDATAESEAIGI 60
           MDID QRILEEFPQS  H+IM++NQ+V+E  P+Q  TCAFC+ VL+  +DAT+E E I I
Sbjct: 1   MDIDLQRILEEFPQSAVHVIMNSNQRVDEHSPIQLPTCAFCQRVLD--SDATSEPEPIDI 60

Query: 61  CGDCKFLLFEDIETPLRDSSHRTTPRGRRTRHGSSESAENLFSQEFTQMINLVRQRQSSI 120
           CGDCKFLL EDIETP+RDS HR TPRGRRT   SSES ENLFSQEFT MINLVRQRQSSI
Sbjct: 61  CGDCKFLLLEDIETPVRDSYHRMTPRGRRTMRSSSESPENLFSQEFTHMINLVRQRQSSI 120

Query: 121 SGHGDRYTDGSNAADFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSLDSMFGESESNFSF 180
           S HGDRY DGSNA DFMQHSSSHTTPNQSRRWRQVYSDSENDGLDS DSMFGESESNFSF
Sbjct: 121 SSHGDRYADGSNAVDFMQHSSSHTTPNQSRRWRQVYSDSENDGLDSFDSMFGESESNFSF 180

Query: 181 GPYRHSYGDSDVVSYRTYGGDSDASLDGHSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVV 240
           GPYRHSYGDSDVVS RTYGGDSDASLDGHSFLDTD F+LP EG D  TDTDIDPMRAG+ 
Sbjct: 181 GPYRHSYGDSDVVSNRTYGGDSDASLDGHSFLDTDIFILPGEGSD--TDTDIDPMRAGIG 240

Query: 241 HWDSSDEEDDRGELTEADTEHDA--------LQDFHVSSATGRGNYGNWNEELSSSEYEG 300
           +WDSSDEEDD GE TEA+TE +         LQ+ +VSSA+ RGN  NW E+LSS EYE 
Sbjct: 241 NWDSSDEEDDHGEFTEAETEQEIGSSEARPQLQNSNVSSASRRGNSSNWTEQLSSPEYE- 300

Query: 301 SARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMRFYSITNYAQSESVSYVGDSGDYLD 360
                                   GS+PG IRRN RFYSITNYAQSE +SYVGDSGDYLD
Sbjct: 301 ------------------------GSTPGHIRRNRRFYSITNYAQSELLSYVGDSGDYLD 360

Query: 361 RQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAICKDFFLVGV 420
           RQ FE+LLEQIAETTSSRRGAPPAA+SFVKNL RLVISKEHLKHDSISCAICKDF LVGV
Sbjct: 361 RQGFEDLLEQIAETTSSRRGAPPAAVSFVKNLPRLVISKEHLKHDSISCAICKDFLLVGV 420

Query: 421 EVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDDRDYEEGKQSSTNAAEIQRLQHQV 480
           EVNQLPCLHLYHP CILPWL+ARNSCPLCRYELPTDDRDYEE K+SS N A    LQ  V
Sbjct: 421 EVNQLPCLHLYHPTCILPWLSARNSCPLCRYELPTDDRDYEEVKRSSINTAATHALQPWV 480

Query: 481 TRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNSYNSGRG-RRSWLFVAAPVVGLVGI 540
             LE+ S LNGVGAN EF++SQ E+  NID AV +S NSGRG RR WLFVAAP+VGLVGI
Sbjct: 481 VGLENPSGLNGVGANSEFESSQEEDYRNIDSAVISSNNSGRGGRRRWLFVAAPIVGLVGI 540

Query: 541 ALMFWVGNP------SGGRITDRRQLDTRGADSSNQREKRTRRWW 570
           ALM W GNP       GG++TD RQLD R A+SSNQRE RTRRWW
Sbjct: 541 ALMLWFGNPLCDQRVPGGQLTDGRQLDARSAESSNQRENRTRRWW 556

BLAST of Cp4.1LG10g07330 vs. TAIR 10
Match: AT5G01980.1 (RING/U-box superfamily protein )

HSP 1 Score: 321.2 bits (822), Expect = 1.6e-87
Identity = 222/542 (40.96%), Postives = 297/542 (54.80%), Query Frame = 0

Query: 38  CAFCRSVLEPENDATAESEAIGICGDCKFLLFEDIETPLRDSSHRTTPRGRR-------T 97
           C  C  V++  +D   + E   +C DCKFLL ED  TP    + R T R RR       T
Sbjct: 24  CNLCGRVVQSASD---DLEIFSLCVDCKFLLLEDFGTPAPPLARRQTIRRRRMRRRRRTT 83

Query: 98  RHGSSES--AENLFSQEFTQMINLVRQRQSSISGHGDRYTDGSNAADFMQHSSSHTTPNQ 157
           RH SSES    +L SQ+FT +I++ RQ  S++    D   D          +SSHTTP+ 
Sbjct: 84  RHDSSESVGVGDLSSQQFTHLISIARQSLSTVQASEDLRDD--------LRTSSHTTPSG 143

Query: 158 SRRWRQVYSDSENDGLDSLDSMFGESESNFSFGPYRHSYGDSDVVSYRTYGGDSDASLDG 217
           S RWR ++S+SE+D  D+    FGE+ESN SF  YR  + D+D +S+  YGG+SDAS D 
Sbjct: 144 STRWR-LFSESESDDFDN----FGETESNASFSLYRFPHADNDTISFSAYGGESDASTDR 203

Query: 218 HSFLDTDNFVLPDEGYDVDTDTDIDPMRAGVVHWDSSDEEDDRGELTEADTEHDALQDFH 277
           H+    D FV PD+  D+D DTDIDPMRAG+  W+S  +E+DR     A     A   + 
Sbjct: 204 HA----DIFVQPDDRSDIDFDTDIDPMRAGLNQWNS--DEEDREWEEGAGPSGVAGTRYR 263

Query: 278 VSSATGRGNYGNWNEELSSSEYEGSARDSITNNARSELGDSGDYLDRQGSSPGQIRRNMR 337
              A+   +Y +      S E E S R  I     S                  + RN+ 
Sbjct: 264 NYLASPSESYSSMT-RFDSPELERSFRQRIIERRHS------------------LSRNI- 323

Query: 338 FYSITNYAQSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLV 397
               T     +   Y  + GDYLD + F+ELLEQ+AE+ +SRRGAPPA++S V+NL R++
Sbjct: 324 ---FTGLEDLDFSPYAANVGDYLDERGFDELLEQLAESDNSRRGAPPASVSCVRNLPRVI 383

Query: 398 ISKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTD 457
           I++EH+    + CAICK+ F +  E  QLPCLHLYH  CI+PWL+ARNSCPLCRYELPTD
Sbjct: 384 IAEEHVM-KGLVCAICKELFSLRNETTQLPCLHLYHAHCIVPWLSARNSCPLCRYELPTD 443

Query: 458 DRDYEEGKQSSTNAAEIQRLQHQVTRLESRSDLNGVGANPEFDTSQGEELSNIDPAVSNS 517
           D+DYEEGK++  + +E           +S S  +G  +  E    +GE  S +       
Sbjct: 444 DKDYEEGKRNVLDVSE-----------DSSSSDDGTESGEEEYVERGESESGVS------ 489

Query: 518 YNSGRGRRSWLFV-AAPVVGLVGIALMFWVGNPSGGRITDRRQLDTRGADSSNQREKRTR 570
               RGR  WLF+ AAPVV LVG+ L  W+ +P       RR +    A S +QRE RTR
Sbjct: 504 -RVSRGR--WLFLAAAPVVSLVGVVLAMWLSSP------HRRGI----AISHSQRENRTR 489

BLAST of Cp4.1LG10g07330 vs. TAIR 10
Match: AT3G19950.1 (RING/U-box superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 6.6e-28
Identity = 56/137 (40.88%), Postives = 81/137 (59.12%), Query Frame = 0

Query: 330 YSITNYAQSESVSYVGDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVI 389
           + I N+         G+ GDY      E+L++Q+AE   +R G PPA+ S +  L  + +
Sbjct: 145 FVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPASKSAIDALPTVKV 204

Query: 390 SKEHLKHDSISCAICKDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELPTDD 449
           +K+ LK +   CA+C D F  G +V Q+PC H++H  C+LPWL   NSCP+CR+ELPTDD
Sbjct: 205 TKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDD 264

Query: 450 RDYEEGKQSSTNAAEIQ 467
            DYE   Q S  + + Q
Sbjct: 265 PDYENRSQGSQGSGDGQ 281

BLAST of Cp4.1LG10g07330 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 108.2 bits (269), Expect = 2.2e-23
Identity = 53/129 (41.09%), Postives = 73/129 (56.59%), Query Frame = 0

Query: 345 GDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAIC 404
           G++GDY      EEL EQ++  T +RRG PPA  S +  L  + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 405 KDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELP------------TDDRDY 462
           KD F +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

BLAST of Cp4.1LG10g07330 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 108.2 bits (269), Expect = 2.2e-23
Identity = 53/129 (41.09%), Postives = 73/129 (56.59%), Query Frame = 0

Query: 345 GDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAIC 404
           G++GDY      EEL EQ++  T +RRG PPA  S +  L  + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 405 KDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELP------------TDDRDY 462
           KD F +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

BLAST of Cp4.1LG10g07330 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 108.2 bits (269), Expect = 2.2e-23
Identity = 53/129 (41.09%), Postives = 73/129 (56.59%), Query Frame = 0

Query: 345 GDSGDYLDRQSFEELLEQIAETTSSRRGAPPAAISFVKNLSRLVISKEHLKHDSISCAIC 404
           G++GDY      EEL EQ++  T +RRG PPA  S +  L  + I++ HL+    +C +C
Sbjct: 135 GNTGDYFFGPGLEELFEQLSAGT-TRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVC 194

Query: 405 KDFFLVGVEVNQLPCLHLYHPPCILPWLTARNSCPLCRYELP------------TDDRDY 462
           KD F +G E  Q+PC H+YH  CI+PWL   NSCP+CR ELP            T  R+Y
Sbjct: 195 KDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPSASGPSSSQNRTTPTRNY 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LPN79.2e-2740.88E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19... [more]
P0CH301.3e-2541.26E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV... [more]
O221973.1e-2241.09Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q9SPL24.0e-2242.97E3 ubiquitin-protein ligase CIP8 OS=Arabidopsis thaliana OX=3702 GN=CIP8 PE=1 SV... [more]
Q94AK47.6e-2139.39E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV... [more]
Match NameE-valueIdentityDescription
XP_023543725.10.099.65E3 ubiquitin-protein ligase Praja-2 isoform X1 [Cucurbita pepo subsp. pepo][more]
KAG7033695.10.098.07E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6603514.10.098.07E3 ubiquitin-protein ligase RING1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022950070.10.098.25E3 ubiquitin-protein ligase Praja-2-like isoform X1 [Cucurbita moschata][more]
XP_022977372.10.095.36E3 ubiquitin-protein ligase Praja-2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GEL60.098.25E3 ubiquitin-protein ligase Praja-2-like isoform X1 OS=Cucurbita moschata OX=366... [more]
A0A6J1II950.095.36E3 ubiquitin-protein ligase Praja-2 OS=Cucurbita maxima OX=3661 GN=LOC111477725 ... [more]
A0A6J1GDT00.093.86uncharacterized protein LOC111453262 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A0A0L2Z49.52e-30074.28RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G337330 P... [more]
A0A5D3C7956.98e-29874.02E3 ubiquitin-protein ligase Praja-2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5... [more]
Match NameE-valueIdentityDescription
AT5G01980.11.6e-8740.96RING/U-box superfamily protein [more]
AT3G19950.16.6e-2840.88RING/U-box superfamily protein [more]
AT2G40830.22.2e-2341.09RING-H2 finger C1A [more]
AT2G40830.12.2e-2341.09RING-H2 finger C1A [more]
AT2G40830.32.2e-2341.09RING-H2 finger C1A [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 401..441
e-value: 2.6E-5
score: 33.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 400..442
e-value: 7.0E-9
score: 35.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 401..442
score: 11.545086
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 362..448
e-value: 1.6E-16
score: 61.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..100
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..95
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 118..152
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 270..306
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 270..320
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 133..152
NoneNo IPR availablePANTHERPTHR15710:SF75E3 UBIQUITIN-PROTEIN LIGASE RING1coord: 340..482
NoneNo IPR availablePANTHERPTHR15710E3 UBIQUITIN-PROTEIN LIGASE PRAJAcoord: 340..482
NoneNo IPR availableCDDcd16667RING-H2_RNF126_likecoord: 400..442
e-value: 1.65643E-21
score: 85.4472
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 390..448

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g07330.1Cp4.1LG10g07330.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0061630 ubiquitin protein ligase activity