Cp4.1LG10g04080 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g04080
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionGermin-like protein
LocationCp4.1LG10: 1653099 .. 1653743 (-)
RNA-Seq ExpressionCp4.1LG10g04080
SyntenyCp4.1LG10g04080
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAATGTGTCTCAAAATCCTCATACCCATCTTCGCATTTCACCTAATTCTCACAACCGTCGTGGCCTCAGATCCCGACCCACTCCAAGATTTCTGCATCCCAAATCCCAAATCCACAAAAAAAGACGACCTTCCATGTAAGAATTCAACCGCCGTCACCGCCGACGACTTCGTCTTCTCCGGCGGAAAATCCGCCGGACCCTTCTCGGAAAGTGGGTTCGCATCGGTAGCGGCAAACCCATCTCTTTTCCCGGGATTGAACACGCTGGGAATGTCGTTCGTACACGCGGATTTCAAGGAGGGGGCCATAAATCCGCCGCACGTGCACCCACGAGCAGCGGAGATGGTGTACGTGGTTCAGGGGAGGGTGTACGCCGGATTTGTTGACTCGGCGAACCGGGTGTACGCGAAGGTGATCGGAGAAGGGGAAGTGATGGTGATTCCGAAGGGGCTGGTGCACTTTCAGATGAACGTGGGGAAGGGAATGGCGAGGGTTTTTGGGTGTTTTAATAGTCAAAATCCGGGGACTCAGAGAATGGCGTCGGCCATATTTGGGTCTGGGATTAAAGAGGAGCTTTTGGAGAAGGCTTTTGGTTTGAGTTCTAATGAGCTTAGGAGGATGAGGAGACGGTTCGAATCTTGA

mRNA sequence

ATGAAAATGTGTCTCAAAATCCTCATACCCATCTTCGCATTTCACCTAATTCTCACAACCGTCGTGGCCTCAGATCCCGACCCACTCCAAGATTTCTGCATCCCAAATCCCAAATCCACAAAAAAAGACGACCTTCCATGTAAGAATTCAACCGCCGTCACCGCCGACGACTTCGTCTTCTCCGGCGGAAAATCCGCCGGACCCTTCTCGGAAAGTGGGTTCGCATCGGTAGCGGCAAACCCATCTCTTTTCCCGGGATTGAACACGCTGGGAATGTCGTTCGTACACGCGGATTTCAAGGAGGGGGCCATAAATCCGCCGCACGTGCACCCACGAGCAGCGGAGATGGTGTACGTGGTTCAGGGGAGGGTGTACGCCGGATTTGTTGACTCGGCGAACCGGGTGTACGCGAAGGTGATCGGAGAAGGGGAAGTGATGGTGATTCCGAAGGGGCTGGTGCACTTTCAGATGAACGTGGGGAAGGGAATGGCGAGGGTTTTTGGGTGTTTTAATAGTCAAAATCCGGGGACTCAGAGAATGGCGTCGGCCATATTTGGGTCTGGGATTAAAGAGGAGCTTTTGGAGAAGGCTTTTGGTTTGAGTTCTAATGAGCTTAGGAGGATGAGGAGACGGTTCGAATCTTGA

Coding sequence (CDS)

ATGAAAATGTGTCTCAAAATCCTCATACCCATCTTCGCATTTCACCTAATTCTCACAACCGTCGTGGCCTCAGATCCCGACCCACTCCAAGATTTCTGCATCCCAAATCCCAAATCCACAAAAAAAGACGACCTTCCATGTAAGAATTCAACCGCCGTCACCGCCGACGACTTCGTCTTCTCCGGCGGAAAATCCGCCGGACCCTTCTCGGAAAGTGGGTTCGCATCGGTAGCGGCAAACCCATCTCTTTTCCCGGGATTGAACACGCTGGGAATGTCGTTCGTACACGCGGATTTCAAGGAGGGGGCCATAAATCCGCCGCACGTGCACCCACGAGCAGCGGAGATGGTGTACGTGGTTCAGGGGAGGGTGTACGCCGGATTTGTTGACTCGGCGAACCGGGTGTACGCGAAGGTGATCGGAGAAGGGGAAGTGATGGTGATTCCGAAGGGGCTGGTGCACTTTCAGATGAACGTGGGGAAGGGAATGGCGAGGGTTTTTGGGTGTTTTAATAGTCAAAATCCGGGGACTCAGAGAATGGCGTCGGCCATATTTGGGTCTGGGATTAAAGAGGAGCTTTTGGAGAAGGCTTTTGGTTTGAGTTCTAATGAGCTTAGGAGGATGAGGAGACGGTTCGAATCTTGA

Protein sequence

MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVFSGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES
Homology
BLAST of Cp4.1LG10g04080 vs. ExPASy Swiss-Prot
Match: Q9SR72 (Germin-like protein subfamily 3 member 2 OS=Arabidopsis thaliana OX=3702 GN=At3g10080 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 5.4e-65
Identity = 125/209 (59.81%), Postives = 158/209 (75.60%), Query Frame = 0

Query: 11  IFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDD------LPCKNSTAVTADDFVFSGGK 70
           +  F++  T  +ASDPDP+QDFCIP P ++   D      LPCKNS+ VT +DFVFSG K
Sbjct: 14  LLCFNVCFT--LASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGLK 73

Query: 71  SAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGRV 130
           +AG F+E+GFA+V   P  FPGLNTLG+SFV AD K G+INPPH HPRA E+ ++V+GRV
Sbjct: 74  TAGNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGRV 133

Query: 131 YAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASAI 190
           Y+GFVDS N+VYAKV+ EGE+MV PKGLVHFQMNVG   A + G  NSQNPG Q++ S +
Sbjct: 134 YSGFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNVGDVTATIVGGLNSQNPGIQKIPSVV 193

Query: 191 FGSGIKEELLEKAFGLSSNELRRMRRRFE 214
           FGSGI EELL KAFGLS  ++  +++RF+
Sbjct: 194 FGSGINEELLMKAFGLSLKQIGTLKKRFD 220

BLAST of Cp4.1LG10g04080 vs. ExPASy Swiss-Prot
Match: Q6ESF0 (Germin-like protein 2-4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0532500 PE=2 SV=1)

HSP 1 Score: 211.8 bits (538), Expect = 7.4e-54
Identity = 110/219 (50.23%), Postives = 149/219 (68.04%), Query Frame = 0

Query: 2   KMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDL------PCKNSTAVTA 61
           + CL +L+ +    L+       DPD +QDFC+P+    +  +L      PC++   +TA
Sbjct: 5   RRCLLLLLAV----LLPAMAARGDPDAVQDFCVPDAGRGRPVELAMLPAYPCRSPANLTA 64

Query: 62  DDFVFSGGKSAGPFS-ESGFASVAANPSLFPGLNTLGMSFVHADFK-EGAINPPHVHPRA 121
            DF FSG ++AG FS E+GFA V+  P+ FPGL+TLGMSF  AD    G +NPPH HPRA
Sbjct: 65  GDFAFSGVRAAGNFSPETGFAGVSVTPAQFPGLHTLGMSFARADLSAAGGVNPPHYHPRA 124

Query: 122 AEMVYVVQGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQ 181
            E   V+ GRVYAGFVDS  R++AKV+ +GEVMV P+ +VHFQ+NVG   A V+G FNS+
Sbjct: 125 TETALVLAGRVYAGFVDSGGRLFAKVLEQGEVMVFPRAMVHFQLNVGDTPATVYGAFNSE 184

Query: 182 NPGTQRMASAIFGSGIKEELLEKAFGLSSNELRRMRRRF 213
           NPG  R+ + +FGSGI+E +LE+AFGL+  ELRR+ +RF
Sbjct: 185 NPGIVRIPATVFGSGIREAVLERAFGLTPAELRRLEKRF 219

BLAST of Cp4.1LG10g04080 vs. ExPASy Swiss-Prot
Match: Q10BU2 (Germin-like protein 3-7 OS=Oryza sativa subsp. japonica OX=39947 GN=GER7 PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.5e-38
Identity = 87/215 (40.47%), Postives = 129/215 (60.00%), Query Frame = 0

Query: 7   ILIPIFAFHLILTTVVASDPDPLQDFCIPNPK-----STKKDDLPCKNSTAVTADDFVFS 66
           +L+   A   +L +  A+DP+P+QDFC+  P+     S      PCK ++AV +DDF F+
Sbjct: 18  LLVLTVAVLAVLASTCAADPEPIQDFCVAVPRAGGEASPAYPGFPCKPASAVVSDDFFFA 77

Query: 67  GGKSAGPFSESGFASV-AANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 126
           G  +AG       AS+   N   FP LNTLG++    D   G +NP H HPRAAE+V+V+
Sbjct: 78  GLAAAGSTDNPFGASLKPGNVEAFPALNTLGVAINRVDLAPGGVNPLHSHPRAAELVHVI 137

Query: 127 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 186
            GR+  GFV +A + Y+KV+GEGE   IP+GL+HFQ N G   AR    FNSQ PG    
Sbjct: 138 TGRMLVGFVSTAGKYYSKVVGEGETFAIPRGLMHFQYNPGNASARAMTVFNSQLPGVVPA 197

Query: 187 ASAIFGSG--IKEELLEKAFGLSSNELRRMRRRFE 214
           A+A+FG+   I + +L K+F + +  ++ ++ +F+
Sbjct: 198 ATALFGADPEIPDAVLAKSFQVDAEIIKLLKSKFK 232

BLAST of Cp4.1LG10g04080 vs. ExPASy Swiss-Prot
Match: Q9FZ27 (Germin-like protein subfamily 2 member 2 OS=Arabidopsis thaliana OX=3702 GN=At1g02335 PE=2 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.3e-38
Identity = 88/210 (41.90%), Postives = 125/210 (59.52%), Query Frame = 0

Query: 6   KILIPIFAFHLILTTVVASDPDPLQDFCIPNPK-STKKDDLPCKNSTAVTADDFVFSG-G 65
           +I I I    +++T++ A DPD LQD C+ +    TK +  PCK +  +T  DF F+G  
Sbjct: 5   RISIIIALSCIMITSIRAYDPDALQDLCVADKSHGTKLNGFPCKETLNITESDFFFAGIS 64

Query: 66  KSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGR 125
           K A   S  G A   AN    PGLNTL +S    D+  G +NPPH HPRA E+VYV++G 
Sbjct: 65  KPAVINSTMGSAVTGANVEKIPGLNTLSVSLARIDYAPGGLNPPHTHPRATEVVYVLEGE 124

Query: 126 VYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASA 185
           +  GF+ +AN+++ K I  GEV V P+GLVHFQ N GK  A V   FNSQ PGT  +A+ 
Sbjct: 125 LEVGFITTANKLFTKTIKIGEVFVFPRGLVHFQKNNGKSPASVLSAFNSQLPGTASVAAT 184

Query: 186 IFGS--GIKEELLEKAFGLSSNELRRMRRR 212
           +F +   + E++L K F + S  + +++ R
Sbjct: 185 LFAAEPALPEDVLTKTFQVGSKMVDKIKER 214

BLAST of Cp4.1LG10g04080 vs. ExPASy Swiss-Prot
Match: P93000 (Germin-like protein subfamily 2 member 3 OS=Arabidopsis thaliana OX=3702 GN=GLP8 PE=2 SV=1)

HSP 1 Score: 158.7 bits (400), Expect = 7.4e-38
Identity = 85/211 (40.28%), Postives = 131/211 (62.09%), Query Frame = 0

Query: 8   LIPIF-AFHLILTTVVASDPDPLQDFCIPN-PKSTKKDDLPCKNSTAVTADDFVFSGGKS 67
           +IPIF  F L+   +  +D + LQDFC+ +     K +  PCK+   VT +DF F G  +
Sbjct: 5   MIPIFVTFMLVAAHMALADTNMLQDFCVADLSNGLKVNGYPCKDPAKVTPEDFYFIGLAT 64

Query: 68  AGPFSES--GFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGR 127
           A   + S  G A   AN    PGLNTLG+S    D+  G +NPPH+HPRA+E ++V++GR
Sbjct: 65  AAATANSSMGSAVTGANVEKVPGLNTLGVSISRIDYAPGGLNPPHLHPRASEAIFVLEGR 124

Query: 128 VYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASA 187
           ++ GF+ +  ++ +K + +G+V V PK L+HFQ N  K  A V   F+SQ PGTQ +  +
Sbjct: 125 LFVGFLTTTGKLISKHVNKGDVFVFPKALLHFQQNPNKAPASVLAAFDSQLPGTQVVGPS 184

Query: 188 IFGSG--IKEELLEKAFGLSSNELRRMRRRF 213
           +FGS   I ++LL KAFG ++ E+++++ +F
Sbjct: 185 LFGSNPPIPDDLLAKAFGAAAPEIQKIKGKF 215

BLAST of Cp4.1LG10g04080 vs. NCBI nr
Match: XP_023545026.1 (germin-like protein subfamily 3 member 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 423 bits (1088), Expect = 2.68e-149
Identity = 214/214 (100.00%), Postives = 214/214 (100.00%), Query Frame = 0

Query: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60
           MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF
Sbjct: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60

Query: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120
           SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV
Sbjct: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120

Query: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180
           QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM
Sbjct: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180

Query: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES
Sbjct: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214

BLAST of Cp4.1LG10g04080 vs. NCBI nr
Match: XP_022977900.1 (germin-like protein subfamily 3 member 2 [Cucurbita maxima])

HSP 1 Score: 407 bits (1047), Expect = 4.79e-143
Identity = 206/214 (96.26%), Postives = 209/214 (97.66%), Query Frame = 0

Query: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60
           MKMCLKILIPIFAFHLILT VVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF
Sbjct: 1   MKMCLKILIPIFAFHLILTIVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60

Query: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120
           SGGKSAGPFSESGFA+VAANPSLFPGLNTLGMSFV ADFKEGAINPPHVHPRAAEMVYVV
Sbjct: 61  SGGKSAGPFSESGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYVV 120

Query: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180
           QGRVYAGFVDSANR+YAKVIGEGEVMVIP+GLVHFQMNVGKG A VFGCFNSQNPGTQRM
Sbjct: 121 QGRVYAGFVDSANRMYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQRM 180

Query: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           ASAIFGSGIKEELLEKAFGLSS ELRRMRRRFES
Sbjct: 181 ASAIFGSGIKEELLEKAFGLSSKELRRMRRRFES 214

BLAST of Cp4.1LG10g04080 vs. NCBI nr
Match: KAG6604355.1 (Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 407 bits (1047), Expect = 4.79e-143
Identity = 205/214 (95.79%), Postives = 210/214 (98.13%), Query Frame = 0

Query: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60
           MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF
Sbjct: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60

Query: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120
           SGGKSAGPFS++GFA+VAANPSLFPGLNTLGMSFV ADFKEGAINPPHVHPRAAEMVYVV
Sbjct: 61  SGGKSAGPFSDNGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYVV 120

Query: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180
           QGRVYAGFVDSANRVYAKVIGEGEVMVIP+GLVHFQMNVGKG A VFGCFNSQNPGTQRM
Sbjct: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQRM 180

Query: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           ASAIFGSGIKEELLEKAFGLSS +LRRMRRRFES
Sbjct: 181 ASAIFGSGIKEELLEKAFGLSSKDLRRMRRRFES 214

BLAST of Cp4.1LG10g04080 vs. NCBI nr
Match: XP_022925793.1 (germin-like protein subfamily 3 member 2 [Cucurbita moschata] >KAG7034505.1 Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 405 bits (1042), Expect = 2.77e-142
Identity = 204/214 (95.33%), Postives = 209/214 (97.66%), Query Frame = 0

Query: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60
           MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPK TKKDDLPCKNSTAVTADDFVF
Sbjct: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKPTKKDDLPCKNSTAVTADDFVF 60

Query: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120
           SGGKSAGPFS++GFA+VAANPSLFPGLNTLGMSFV ADFKEGAINPPHVHPRAAEMVYVV
Sbjct: 61  SGGKSAGPFSDNGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYVV 120

Query: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180
           QGRVYAGFVDSANRVYAKVIGEGEVMVIP+GLVHFQMNVGKG A VFGCFNSQNPGTQRM
Sbjct: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQRM 180

Query: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           ASAIFGSGIKEELLEKAFGLSS +LRRMRRRFES
Sbjct: 181 ASAIFGSGIKEELLEKAFGLSSKDLRRMRRRFES 214

BLAST of Cp4.1LG10g04080 vs. NCBI nr
Match: XP_038883343.1 (germin-like protein subfamily 3 member 2 [Benincasa hispida])

HSP 1 Score: 332 bits (851), Expect = 2.77e-113
Identity = 177/214 (82.71%), Postives = 187/214 (87.38%), Query Frame = 0

Query: 3   MCLKILIPIFAFHLILTTVVASDPDPLQDFCIP--NPKSTKKDDLPCKNSTAVTADDFVF 62
           M LKILIPI    LILT  +ASDPDPLQDFCIP  NPKS  K    CKNSTAVTA+DF+F
Sbjct: 1   MSLKILIPI----LILTIAMASDPDPLQDFCIPISNPKSPNK---LCKNSTAVTANDFIF 60

Query: 63  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 122
           SGGKS G FSESGFA+V+ANPS FPGLNTLGMSFV ADFKEGAINPPHVHPR AEMVYVV
Sbjct: 61  SGGKSCGSFSESGFATVSANPSNFPGLNTLGMSFVRADFKEGAINPPHVHPRGAEMVYVV 120

Query: 123 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 182
           QGRVY GFVDSANRVYAKVI EGEVMVIP+GLVHFQMNVGK  A VFGCFNSQNPGTQ+M
Sbjct: 121 QGRVYVGFVDSANRVYAKVIEEGEVMVIPRGLVHFQMNVGKRKATVFGCFNSQNPGTQKM 180

Query: 183 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           A+AIFGSGIKEELLEKAFGLSS E+RRM+R FES
Sbjct: 181 AAAIFGSGIKEELLEKAFGLSSKEIRRMKRMFES 207

BLAST of Cp4.1LG10g04080 vs. ExPASy TrEMBL
Match: A0A6J1INL7 (Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111478053 PE=3 SV=1)

HSP 1 Score: 407 bits (1047), Expect = 2.32e-143
Identity = 206/214 (96.26%), Postives = 209/214 (97.66%), Query Frame = 0

Query: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60
           MKMCLKILIPIFAFHLILT VVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF
Sbjct: 1   MKMCLKILIPIFAFHLILTIVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60

Query: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120
           SGGKSAGPFSESGFA+VAANPSLFPGLNTLGMSFV ADFKEGAINPPHVHPRAAEMVYVV
Sbjct: 61  SGGKSAGPFSESGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYVV 120

Query: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180
           QGRVYAGFVDSANR+YAKVIGEGEVMVIP+GLVHFQMNVGKG A VFGCFNSQNPGTQRM
Sbjct: 121 QGRVYAGFVDSANRMYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQRM 180

Query: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           ASAIFGSGIKEELLEKAFGLSS ELRRMRRRFES
Sbjct: 181 ASAIFGSGIKEELLEKAFGLSSKELRRMRRRFES 214

BLAST of Cp4.1LG10g04080 vs. ExPASy TrEMBL
Match: A0A6J1EG93 (Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433096 PE=3 SV=1)

HSP 1 Score: 405 bits (1042), Expect = 1.34e-142
Identity = 204/214 (95.33%), Postives = 209/214 (97.66%), Query Frame = 0

Query: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVF 60
           MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPK TKKDDLPCKNSTAVTADDFVF
Sbjct: 1   MKMCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKPTKKDDLPCKNSTAVTADDFVF 60

Query: 61  SGGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVV 120
           SGGKSAGPFS++GFA+VAANPSLFPGLNTLGMSFV ADFKEGAINPPHVHPRAAEMVYVV
Sbjct: 61  SGGKSAGPFSDNGFATVAANPSLFPGLNTLGMSFVRADFKEGAINPPHVHPRAAEMVYVV 120

Query: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRM 180
           QGRVYAGFVDSANRVYAKVIGEGEVMVIP+GLVHFQMNVGKG A VFGCFNSQNPGTQRM
Sbjct: 121 QGRVYAGFVDSANRVYAKVIGEGEVMVIPRGLVHFQMNVGKGTATVFGCFNSQNPGTQRM 180

Query: 181 ASAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           ASAIFGSGIKEELLEKAFGLSS +LRRMRRRFES
Sbjct: 181 ASAIFGSGIKEELLEKAFGLSSKDLRRMRRRFES 214

BLAST of Cp4.1LG10g04080 vs. ExPASy TrEMBL
Match: A0A1S3CIT4 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103500894 PE=3 SV=1)

HSP 1 Score: 328 bits (841), Expect = 4.62e-112
Identity = 174/213 (81.69%), Postives = 185/213 (86.85%), Query Frame = 0

Query: 3   MCLKI-LIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVFS 62
           MCLKI LIPI    LILT  +ASDPDPLQDFCIPNPKS       CKNSTAVTADDFVFS
Sbjct: 1   MCLKIVLIPIL---LILTIAMASDPDPLQDFCIPNPKSFPNQ--LCKNSTAVTADDFVFS 60

Query: 63  GGKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQ 122
           G KSAGPFSE+GFA+VAANP  FPGLNTLGMS V  D KEGAINPPHVHPRAAEMVYVVQ
Sbjct: 61  GAKSAGPFSETGFATVAANPINFPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVQ 120

Query: 123 GRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMA 182
           G+VYAGFVDS NRVYAKVI EGEVMVIP+G+VHFQMNVGK  A VFGCFNSQNPGTQ+MA
Sbjct: 121 GKVYAGFVDSGNRVYAKVIEEGEVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMA 180

Query: 183 SAIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           +AIFGSGIKEELLEKAFGLSS E+RRM+R F+S
Sbjct: 181 AAIFGSGIKEELLEKAFGLSSKEIRRMKRMFDS 208

BLAST of Cp4.1LG10g04080 vs. ExPASy TrEMBL
Match: A0A0A0KHH1 (Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G452110 PE=3 SV=1)

HSP 1 Score: 318 bits (816), Expect = 2.87e-108
Identity = 169/212 (79.72%), Postives = 181/212 (85.38%), Query Frame = 0

Query: 3   MCLKILIPIFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVFSG 62
           M LKI I I    LILT  +ASDPDPLQDFCIPNPKS    D  CKNSTAVTADDFVFSG
Sbjct: 1   MFLKIPISIL---LILTIAIASDPDPLQDFCIPNPKSFP--DQLCKNSTAVTADDFVFSG 60

Query: 63  GKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQG 122
            KSAGPFS +GFA+VAAN   FPGLNTLGMS V  D KEGAINPPHVHPRAAEMVYVV+G
Sbjct: 61  AKSAGPFSGTGFATVAANSINFPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVEG 120

Query: 123 RVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMAS 182
           +VYAGFVDS NRVYAKVI EGEVMVIP+G+VHFQMNVGK  A VFGCFNSQNPGTQ+MA+
Sbjct: 121 KVYAGFVDSGNRVYAKVIEEGEVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMAA 180

Query: 183 AIFGSGIKEELLEKAFGLSSNELRRMRRRFES 214
           AIFGSGIKEELLEKAFGLSS E+RRM+R F+S
Sbjct: 181 AIFGSGIKEELLEKAFGLSSKEIRRMKRMFDS 207

BLAST of Cp4.1LG10g04080 vs. ExPASy TrEMBL
Match: A0A5A7SGJ6 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001100 PE=3 SV=1)

HSP 1 Score: 317 bits (813), Expect = 4.70e-108
Identity = 161/193 (83.42%), Postives = 172/193 (89.12%), Query Frame = 0

Query: 22  VASDPDPLQDFCIPNPKSTKKDDLPCKNSTAVTADDFVFSGGKSAGPFSESGFASVAANP 81
           +ASDPDPLQDFCIPNPKS       CKNSTAVTADDFVFSG KSAGPFSE+GFA+VAANP
Sbjct: 1   MASDPDPLQDFCIPNPKSFPNQ--LCKNSTAVTADDFVFSGAKSAGPFSETGFATVAANP 60

Query: 82  SLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGRVYAGFVDSANRVYAKVIG 141
             FPGLNTLGMS V  D KEGAINPPHVHPRAAEMVYVVQG+VYAGFVDS NRVYAKVI 
Sbjct: 61  INFPGLNTLGMSLVRVDLKEGAINPPHVHPRAAEMVYVVQGKVYAGFVDSGNRVYAKVIE 120

Query: 142 EGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASAIFGSGIKEELLEKAFGLS 201
           EGEVMVIP+G+VHFQMNVGK  A VFGCFNSQNPGTQ+MA+AIFGSGIKEELLEKAFGLS
Sbjct: 121 EGEVMVIPRGVVHFQMNVGKRRATVFGCFNSQNPGTQKMAAAIFGSGIKEELLEKAFGLS 180

Query: 202 SNELRRMRRRFES 214
           S E+RRM+R F+S
Sbjct: 181 SKEIRRMKRMFDS 191

BLAST of Cp4.1LG10g04080 vs. TAIR 10
Match: AT3G10080.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 248.8 bits (634), Expect = 3.9e-66
Identity = 125/209 (59.81%), Postives = 158/209 (75.60%), Query Frame = 0

Query: 11  IFAFHLILTTVVASDPDPLQDFCIPNPKSTKKDD------LPCKNSTAVTADDFVFSGGK 70
           +  F++  T  +ASDPDP+QDFCIP P ++   D      LPCKNS+ VT +DFVFSG K
Sbjct: 14  LLCFNVCFT--LASDPDPIQDFCIPKPVTSPYHDHHFSTNLPCKNSSEVTTEDFVFSGLK 73

Query: 71  SAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGRV 130
           +AG F+E+GFA+V   P  FPGLNTLG+SFV AD K G+INPPH HPRA E+ ++V+GRV
Sbjct: 74  TAGNFTETGFATVPVGPENFPGLNTLGISFVRADLKPGSINPPHYHPRATEVAHLVKGRV 133

Query: 131 YAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASAI 190
           Y+GFVDS N+VYAKV+ EGE+MV PKGLVHFQMNVG   A + G  NSQNPG Q++ S +
Sbjct: 134 YSGFVDSNNKVYAKVMEEGEMMVYPKGLVHFQMNVGDVTATIVGGLNSQNPGIQKIPSVV 193

Query: 191 FGSGIKEELLEKAFGLSSNELRRMRRRFE 214
           FGSGI EELL KAFGLS  ++  +++RF+
Sbjct: 194 FGSGINEELLMKAFGLSLKQIGTLKKRFD 220

BLAST of Cp4.1LG10g04080 vs. TAIR 10
Match: AT1G02335.1 (germin-like protein subfamily 2 member 2 precursor )

HSP 1 Score: 159.8 bits (403), Expect = 2.4e-39
Identity = 88/210 (41.90%), Postives = 125/210 (59.52%), Query Frame = 0

Query: 6   KILIPIFAFHLILTTVVASDPDPLQDFCIPNPK-STKKDDLPCKNSTAVTADDFVFSG-G 65
           +I I I    +++T++ A DPD LQD C+ +    TK +  PCK +  +T  DF F+G  
Sbjct: 5   RISIIIALSCIMITSIRAYDPDALQDLCVADKSHGTKLNGFPCKETLNITESDFFFAGIS 64

Query: 66  KSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGR 125
           K A   S  G A   AN    PGLNTL +S    D+  G +NPPH HPRA E+VYV++G 
Sbjct: 65  KPAVINSTMGSAVTGANVEKIPGLNTLSVSLARIDYAPGGLNPPHTHPRATEVVYVLEGE 124

Query: 126 VYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASA 185
           +  GF+ +AN+++ K I  GEV V P+GLVHFQ N GK  A V   FNSQ PGT  +A+ 
Sbjct: 125 LEVGFITTANKLFTKTIKIGEVFVFPRGLVHFQKNNGKSPASVLSAFNSQLPGTASVAAT 184

Query: 186 IFGS--GIKEELLEKAFGLSSNELRRMRRR 212
           +F +   + E++L K F + S  + +++ R
Sbjct: 185 LFAAEPALPEDVLTKTFQVGSKMVDKIKER 214

BLAST of Cp4.1LG10g04080 vs. TAIR 10
Match: AT3G05930.1 (germin-like protein 8 )

HSP 1 Score: 158.7 bits (400), Expect = 5.3e-39
Identity = 85/211 (40.28%), Postives = 131/211 (62.09%), Query Frame = 0

Query: 8   LIPIF-AFHLILTTVVASDPDPLQDFCIPN-PKSTKKDDLPCKNSTAVTADDFVFSGGKS 67
           +IPIF  F L+   +  +D + LQDFC+ +     K +  PCK+   VT +DF F G  +
Sbjct: 5   MIPIFVTFMLVAAHMALADTNMLQDFCVADLSNGLKVNGYPCKDPAKVTPEDFYFIGLAT 64

Query: 68  AGPFSES--GFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGR 127
           A   + S  G A   AN    PGLNTLG+S    D+  G +NPPH+HPRA+E ++V++GR
Sbjct: 65  AAATANSSMGSAVTGANVEKVPGLNTLGVSISRIDYAPGGLNPPHLHPRASEAIFVLEGR 124

Query: 128 VYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASA 187
           ++ GF+ +  ++ +K + +G+V V PK L+HFQ N  K  A V   F+SQ PGTQ +  +
Sbjct: 125 LFVGFLTTTGKLISKHVNKGDVFVFPKALLHFQQNPNKAPASVLAAFDSQLPGTQVVGPS 184

Query: 188 IFGSG--IKEELLEKAFGLSSNELRRMRRRF 213
           +FGS   I ++LL KAFG ++ E+++++ +F
Sbjct: 185 LFGSNPPIPDDLLAKAFGAAAPEIQKIKGKF 215

BLAST of Cp4.1LG10g04080 vs. TAIR 10
Match: AT1G18980.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.2e-37
Identity = 90/217 (41.47%), Postives = 131/217 (60.37%), Query Frame = 0

Query: 5   LKILIPIFAFHLILTTV-----VASDPDPLQDFCIPNPKSTKK-DDLPCKNSTAVTADDF 64
           L+I   +F   L++  V     ++SD DPLQDFC+ + K++   +  PCK+S  V+A DF
Sbjct: 4   LQISSSLFRSFLLVICVFVIPSLSSDSDPLQDFCVGDLKASPSINGFPCKSS--VSASDF 63

Query: 65  VFSG-GKSAGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMV 124
            FSG G      + +G A   AN   FPGLNTLG+S  + +F  G +NPPH HPRA E  
Sbjct: 64  FFSGLGGPLNTSTPNGVAVSPANVLTFPGLNTLGLSMNNVEFAPGGVNPPHSHPRATEAG 123

Query: 125 YVVQGRVYAGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGT 184
            V++G V+ GF+ + N +++KV+  GE+ V+P+GLVHFQ NVGK  AR+   FNSQ PG+
Sbjct: 124 VVIEGSVFVGFLTTNNTLFSKVLNAGEMFVVPRGLVHFQWNVGKVKARLITSFNSQLPGS 183

Query: 185 QRMASAIFGSG--IKEELLEKAFGLSSNELRRMRRRF 213
             + S +FGS   I   +L K F      + +++ +F
Sbjct: 184 AVLPSTLFGSNPTIPNAVLTKTFRTDDVTVNKLKSKF 218

BLAST of Cp4.1LG10g04080 vs. TAIR 10
Match: AT5G26700.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.2e-37
Identity = 77/207 (37.20%), Postives = 127/207 (61.35%), Query Frame = 0

Query: 12  FAFHLILTT----VVASDPDPLQDFCIPN-PKSTKKDDLPCKNSTAVTADDFVFSG-GKS 71
           FA HL++      V  +  + LQD C+ +   + K +   CK+ST +T +DF F G    
Sbjct: 4   FATHLVVVVTMLFVAMASAEMLQDVCVADLSNAVKVNGYTCKDSTQITPEDFYFKGLANI 63

Query: 72  AGPFSESGFASVAANPSLFPGLNTLGMSFVHADFKEGAINPPHVHPRAAEMVYVVQGRVY 131
           A   + +G     AN    PGLNTLG+S    D+    +NPPHVHPRA+E+++V++G++Y
Sbjct: 64  AATNTSTGSVVTGANVEKLPGLNTLGLSMSRIDYAPNGLNPPHVHPRASEIIFVLEGQLY 123

Query: 132 AGFVDSANRVYAKVIGEGEVMVIPKGLVHFQMNVGKGMARVFGCFNSQNPGTQRMASAIF 191
            GFV +A ++ AK + +G+V   PKGL+HFQ N+    A V   F+SQ PGTQ + +++F
Sbjct: 124 VGFVTTAGKLIAKNLNKGDVFTFPKGLIHFQKNIANSPASVLAAFDSQLPGTQSLVASLF 183

Query: 192 GSGIKEELLEKAFGLSSNELRRMRRRF 213
           G+ + +++L K+F L   ++++++ R+
Sbjct: 184 GA-LPDDILAKSFQLKHKQVKKIKLRY 209

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SR725.4e-6559.81Germin-like protein subfamily 3 member 2 OS=Arabidopsis thaliana OX=3702 GN=At3g... [more]
Q6ESF07.4e-5450.23Germin-like protein 2-4 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0532500... [more]
Q10BU22.5e-3840.47Germin-like protein 3-7 OS=Oryza sativa subsp. japonica OX=39947 GN=GER7 PE=2 SV... [more]
Q9FZ273.3e-3841.90Germin-like protein subfamily 2 member 2 OS=Arabidopsis thaliana OX=3702 GN=At1g... [more]
P930007.4e-3840.28Germin-like protein subfamily 2 member 3 OS=Arabidopsis thaliana OX=3702 GN=GLP8... [more]
Match NameE-valueIdentityDescription
XP_023545026.12.68e-149100.00germin-like protein subfamily 3 member 2 [Cucurbita pepo subsp. pepo][more]
XP_022977900.14.79e-14396.26germin-like protein subfamily 3 member 2 [Cucurbita maxima][more]
KAG6604355.14.79e-14395.79Germin-like protein subfamily 3 member 2, partial [Cucurbita argyrosperma subsp.... [more]
XP_022925793.12.77e-14295.33germin-like protein subfamily 3 member 2 [Cucurbita moschata] >KAG7034505.1 Germ... [more]
XP_038883343.12.77e-11382.71germin-like protein subfamily 3 member 2 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1INL72.32e-14396.26Germin-like protein OS=Cucurbita maxima OX=3661 GN=LOC111478053 PE=3 SV=1[more]
A0A6J1EG931.34e-14295.33Germin-like protein OS=Cucurbita moschata OX=3662 GN=LOC111433096 PE=3 SV=1[more]
A0A1S3CIT44.62e-11281.69Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103500894 PE=3 SV=1[more]
A0A0A0KHH12.87e-10879.72Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_6G452110 PE=3 SV=1[more]
A0A5A7SGJ64.70e-10883.42Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G... [more]
Match NameE-valueIdentityDescription
AT3G10080.13.9e-6659.81RmlC-like cupins superfamily protein [more]
AT1G02335.12.4e-3941.90germin-like protein subfamily 2 member 2 precursor [more]
AT3G05930.15.3e-3940.28germin-like protein 8 [more]
AT1G18980.12.2e-3741.47RmlC-like cupins superfamily protein [more]
AT5G26700.12.2e-3737.20RmlC-like cupins superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001929GerminPRINTSPR00325GERMINcoord: 108..128
score: 50.5
coord: 138..158
score: 52.16
coord: 171..186
score: 48.26
IPR006045Cupin 1SMARTSM00835Cupin_1_3coord: 58..206
e-value: 3.1E-42
score: 156.3
IPR006045Cupin 1PFAMPF00190Cupin_1coord: 69..204
e-value: 1.8E-39
score: 134.7
IPR014710RmlC-like jelly roll foldGENE3D2.60.120.10Jelly Rollscoord: 24..214
e-value: 4.3E-68
score: 230.7
NoneNo IPR availablePIRSRPIRSR617774-2PIRSR617774-2coord: 92..208
e-value: 1.1E-6
score: 25.7
NoneNo IPR availablePANTHERPTHR31238:SF8GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 2coord: 11..213
NoneNo IPR availablePANTHERPTHR31238GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3coord: 11..213
NoneNo IPR availableCDDcd02241cupin_OxOxcoord: 25..212
e-value: 2.38093E-78
score: 231.331
IPR011051RmlC-like cupin domain superfamilySUPERFAMILY51182RmlC-like cupinscoord: 56..212

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g04080.1Cp4.1LG10g04080.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0048046 apoplast
molecular_function GO:0030145 manganese ion binding