Cp4.1LG10g03120 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG10g03120
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	iron-sulfur assembly protein IscA, chloroplastic-like
Location	Cp4.1LG10: 2222210 .. 2225070 (-)
RNA-Seq Expression	Cp4.1LG10g03120
Synteny	Cp4.1LG10g03120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAACCACTCGATCTTAAAGAAAACAATATATCCTGACCGCACGATAACGATTCGCAAACATTTCCGACTAGTAAAAGGTGGCGGTTCGCATATAAACCTTTCGCCTTCGCCGTTCGCCGGACTAATCGCGACCGGTTTTCTTCGATGGCGTTCTCTTCGATTACCATGAAATGCCCCCCTGTTCTACACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCTATTCGATCGGTTTCAGTCCCAGGTACGCCTCTATTTTATTTTTTCCCCTCTGCTTTCCTCCTTGATTTTTGGGTATTATATTTGACTGTATCACCGTAGTTCATCGTTGTACTGTTTGGGGGAAAATTAATCAGGAATCTGTTTCTTTTGGATTGGAACTATGAACTTGGGAAGAACGTCAGGATTCGAGTTATTCTTTTAATTAATTAAACGCGGGATTCAATTTCTGGTTGCTTGGGATCGAAATTGTTAGCTCGTGGAAGTTTTATATTGAAATTAAGAAACAGAATTTTGGGGATTTTCTTATTGAGTTACACGGAAAATAAATTTCCCTGCTACTTGGAACGGATGATTTGTTTGTGAGTTCTAATTCGAGATCCAGTTAGTGATATACAAACCATGAGTGTGCATATTTGTTTTGTATCAATGCCTAAATGATTAGGATGGATTGATCTTCATGTGGGAGCAAAGATGTTTACTAATTCTTGTGTTTGATAGCATTGATCTTTAATTCTTTTTTAGAGCATACAGAATGGATTTGAAATGATTAAACAACTATCTTTCATGTTGAAACCACTCCGAAATATGTCTTAAATCAATTGAATGTTTGCTTTTATACTTTTAAATGCAATTTTCATACTATTTGAATGATTATAAGTATGTTTTGGAGTGATTTTGAGTATGAAAAAAAGTGATTTCAACCAATTTCAAAATCACTCCTAAACATGACATGTAATATGGTATTGTGAATGGCACTTTACCTTTGCCGCTTACTAGAGATCAAAGATAGAATCTACTGAATCTTGCAGTTTCTGCATATGAACTATGATGAGTATATTATGCGTTATGATCACAGTTTTGCATTATCATCTTATGTTGTTGAACATGTAAATTGTGATTTAACAGCTGCGCCAGCATCTGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGGTTTGTGATTGGTAAGAGTTATAGCTTTTCGTTTTTCGCTCTGAATTTCTAGTGGGGTTCTTAGATTTATCCTTTTCGAATTCATTATTTTTGTAGATGACCCATTTCTTTATATATATATATATATACTCTTAGTTCTATGCCTTCAAAAGAACATGGTGGATCTGAGTTGAGTTTTGCATGTTTTTGGATCGATACTTTCTTTCCCATGGAATGGTAGAGGGAAAGTTTTCAATTCTTCTTAGGATGAAAGAAGCTTACACTTTTATAAATTCGGATATAGACGGGCATTTCGTGAGCCATATGCCATAAATTTGAAGGACTCAATGTAACAGCTCAAGCCCACCGCTAGCAAATATTGTCCGCTTTGGCCCGTTACGTATCATCGTCAGCCTCACTGTTTTAACACTCGACTATTAGGGAGATGTTTCCACATTCTTATAAGCAATGCTTCGTTCTCCTCTCCAATCGTTGTGGGATCTCACAATCCACCCCCCTTGGGGACTAGCGTTCTCGTTGGTACACCGCTCAGTGTCTACCTCAGATACCATTTGTAATAACTCAAGCCCACCACTAGCATATATTGTTTGCTTTGGATTGAGAGATCCCACATCGATTGGAGAGGAGAACGAAACATTACTTATAAGAGCATGGAAATCTCTCCCTAATAGACGCGTGTTAAAAACGTGAAGCTGACGTCGATACGTAACGGGCCAAAGCAGACAATGTTTGTTAGCAGTGAGCTTGAACTAATAATTTTACTCAATAGTTCATAGTCTATCTCCTCTTGGACACTCGATGACTTGAAGGAATTGATATCATAAGTTTTTTTATGCTGACTGATAAGCAGGTTGTGCAACTTTTGTCAAATATACTTTTCTTTAATGTATACTATTTCAACATGAGCTTGTATATTGTTTCGATTCCTTGACAAATGCTCTCCTACTCTATTGATAAAGGAACCTAAGCATCTTTCAGTGAACTATGAAGGTATCATTTTATCTACTTCATTTTCGACTGTGCCCTCTTAAGTCGTAGGCTCGACGACGAATATCAGTTCACTTATATCACTTCCTTTATAGCTTGAGCTTTTGATACAAACTTCCCTCTCTGCTTGTTTTGTAGTATGCGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAGTCATTTGCTGCAGAAATGTAGGATCATCATTTGTATATATATATATATATGAATGATTCAACACAAACTTGATAGTTTCATATTAAAGAAAACTTAGAACGTTGTATAAACTCTTAGTTTGGTAGGCGCTCGATCCTAATAACATACCTTCTATTACAAGTCTTGTGTTCGTGATGTTTCTGCTTCAGCCCTTCTGGAAAAATTAGCTCTTCCTGCCTAC

mRNA sequence

TGAACCACTCGATCTTAAAGAAAACAATATATCCTGACCGCACGATAACGATTCGCAAACATTTCCGACTAGTAAAAGGTGGCGGTTCGCATATAAACCTTTCGCCTTCGCCGTTCGCCGGACTAATCGCGACCGGTTTTCTTCGATGGCGTTCTCTTCGATTACCATGAAATGCCCCCCTGTTCTACACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCTATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCTGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGGTTTGTGATTGTATGCGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAGTCATTTGCTGCAGAAATGTAGGATCATCATTTGTATATATATATATATATGAATGATTCAACACAAACTTGATAGTTTCATATTAAAGAAAACTTAGAACGTTGTATAAACTCTTAGTTTGGTAGGCGCTCGATCCTAATAACATACCTTCTATTACAAGTCTTGTGTTCGTGATGTTTCTGCTTCAGCCCTTCTGGAAAAATTAGCTCTTCCTGCCTAC

Coding sequence (CDS)

ATGGCGTTCTCTTCGATTACCATGAAATGCCCCCCTGTTCTACACCTGCGAGCCCTTCCTAAAAGCTCTGCACCTCAAAGTTCAATTTCCTTTCGATATTCACCTACGAAATTCAGCCGGAGAAAAACCTTATCTATTCGATCGGTTTCAGTCCCAGCTGCGCCAGCATCTGAGGGTATAGCACCGGCTATCTCATTAACCGATAATGCATTGAAACACTTGAATAAAATGAGATCTGAACGTAATGAAGATTTGTGCTTGAGAATTGGTGTCAGACAGGGTGGATGCTCTGGTATGTCCTACACGATGGAGTTTGAGAACAGGGCAAATGCTAGACCTGATGACTCAATTATAGAATATAATGGGTTTGTGATTGTATGCGATCCCAAGAGCCTACTTTTCTTGTTTGGAATGCAATTGGACTACAGCGACGCACTTATTGGTGGGGGCTTCTCCTTCAAGAACCCAAACGCTACACAAACTTGTGGCTGTGGAAAGTCATTTGCTGCAGAAATGTAG

Protein sequence

MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM

Homology

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BLAST of Cp4.1LG10g03120 vs. ExPASy Swiss-Prot
Match: Q9XIK3 (Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISCA PE=2 SV=2)

HSP 1 Score: 244.2 bits (622), Expect = 1.1e-63
Identity = 120/149 (80.54%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 27  SSISF---RYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENRANARPDDS IEY GF IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           DALIGGGFSF NPNATQTCGCGKSFAAEM
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy Swiss-Prot
Match: P72731 (Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=slr1417 PE=3 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.3e-36
Identity = 68/108 (62.96%), Postives = 83/108 (76.85%), Query Frame = 0


Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           A  I L+D ALKHL  ++ ++ +DLCLR+GVRQGGCSGMSY M+FE    A   D + +Y
Sbjct: 9   AKGIQLSDAALKHLLALKEQQGKDLCLRVGVRQGGCSGMSYMMDFEEPNRATEHDEVFDY 68

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            GF I+CD KSLL+L+G+ LDYS+ALIGGGF F NPNA QTCGCGKSF
Sbjct: 69  EGFQIICDRKSLLYLYGLMLDYSNALIGGGFQFTNPNANQTCGCGKSF 116

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy Swiss-Prot
Match: P51217 (Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 3.7e-32
Identity = 59/106 (55.66%), Postives = 83/106 (78.30%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           I+LT+ A + +  ++S  + ++CLRIGV+QGGCSGMSY+M FE+ ++ R  D ++  + F
Sbjct: 8   ITLTEPAARQIKILKSNFDNEVCLRIGVKQGGCSGMSYSMNFESVSSLRETDEVLMLDSF 67

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           ++ CDPKSLL+L+G+ LDYS  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 68  LVACDPKSLLYLYGLSLDYSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy Swiss-Prot
Match: Q1XDR9 (Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-30
Identity = 61/112 (54.46%), Postives = 81/112 (72.32%), Query Frame = 0


Query: 58  EGIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSI 117
           EG    I++T  ALK +  ++++   D+ LRIGVRQGGCSGMSY+M FE+    +  D  
Sbjct: 2   EGNMGFITITKPALKQIAILKNDHENDVHLRIGVRQGGCSGMSYSMNFEHVDKLKDTDER 61

Query: 118 IEYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           +  + F +VCDPKSLL+L+G+ LD+S  LIGGGF F NPNA+QTCGCGKSF+
Sbjct: 62  LRLDNFSVVCDPKSLLYLYGLSLDFSSELIGGGFQFSNPNASQTCGCGKSFS 113

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy Swiss-Prot
Match: Q9MSA1 (Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 7.8e-30
Identity = 58/107 (54.21%), Postives = 81/107 (75.70%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNED-LCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNG 123
           I++T +AL HLN +    +ED L +RIG++QGGCSG+SY M +E ++N   +D + +Y+ 
Sbjct: 6   INITKSALDHLNSISKLNSEDKLYIRIGIKQGGCSGLSYFMTYEKKSNISDNDLVYDYDN 65

Query: 124 FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFA 170
           F +VCD KS+L+L+G+ LDYS +LI GGF F NPNA QTCGCGKSF+
Sbjct: 66  FTLVCDNKSILYLYGISLDYSSSLIDGGFKFLNPNAKQTCGCGKSFS 112

[Close]

BLAST of Cp4.1LG10g03120 vs. NCBI nr
Match: XP_022950286.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_023544833.1 iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita pepo subsp. pepo] >KAG6604246.1 Iron-sulfur assembly protein IscA, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 347 bits (889), Expect = 2.40e-120
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. NCBI nr
Match: KAG7034403.1 (Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 345 bits (885), Expect = 9.78e-120
Identity = 171/172 (99.42%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSIT+KCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITVKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. NCBI nr
Match: XP_022977954.1 (iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 342 bits (876), Expect = 2.31e-118
Identity = 170/172 (98.84%), Postives = 170/172 (98.84%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. NCBI nr
Match: XP_038880838.1 (iron-sulfur assembly protein IscA, chloroplastic [Benincasa hispida])

HSP 1 Score: 337 bits (865), Expect = 1.10e-116
Identity = 166/172 (96.51%), Postives = 171/172 (99.42%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKC P+LHLRALP+SSAPQSSISFRYSPTKFSRRK+LSIRSVSVPAAPA+EGI
Sbjct: 1   MAFSSITMKCTPILHLRALPQSSAPQSSISFRYSPTKFSRRKSLSIRSVSVPAAPATEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAE+
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEV 172

[Close]

BLAST of Cp4.1LG10g03120 vs. NCBI nr
Match: XP_008440129.1 (PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo])

HSP 1 Score: 330 bits (847), Expect = 6.10e-114
Identity = 165/172 (95.93%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMK PPVLHLRALPKSSAPQSSISFR+SPTKFSR K+LSIRSVSVPAAPASE I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy TrEMBL
Match: A0A6J1GEF0 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111453419 PE=3 SV=1)

HSP 1 Score: 347 bits (889), Expect = 1.16e-120
Identity = 172/172 (100.00%), Postives = 172/172 (100.00%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy TrEMBL
Match: A0A6J1ISS5 (iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111478089 PE=3 SV=1)

HSP 1 Score: 342 bits (876), Expect = 1.12e-118
Identity = 170/172 (98.84%), Postives = 170/172 (98.84%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI
Sbjct: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENR NARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRENARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           N FVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM
Sbjct: 121 NEFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy TrEMBL
Match: A0A1S3B105 (iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103484688 PE=3 SV=1)

HSP 1 Score: 330 bits (847), Expect = 2.95e-114
Identity = 165/172 (95.93%), Postives = 168/172 (97.67%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSSITMK PPVLHLRALPKSSAPQSSISFR+SPTKFSR K+LSIRSVSVPAAPASE I
Sbjct: 1   MAFSSITMKFPPVLHLRALPKSSAPQSSISFRFSPTKFSRSKSLSIRSVSVPAAPASESI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNALKHLNKMRSER+EDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALKHLNKMRSERDEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy TrEMBL
Match: A0A6J1BTR2 (iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111005271 PE=3 SV=1)

HSP 1 Score: 321 bits (823), Expect = 1.35e-110
Identity = 160/172 (93.02%), Postives = 165/172 (95.93%), Query Frame = 0


Query: 1   MAFSSITMKCPPVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGI 60
           MAFSS TMK P VLHLRALP+SSA QSSISFRYS TKFSRRK+LS+RSVSVPAAP SEGI
Sbjct: 1   MAFSSATMKFPSVLHLRALPESSATQSSISFRYSLTKFSRRKSLSVRSVSVPAAPTSEGI 60

Query: 61  APAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120
           APAISLTDNAL HLNKMRS+RNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY
Sbjct: 61  APAISLTDNALNHLNKMRSDRNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEY 120

Query: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAA+M
Sbjct: 121 NGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAADM 172

[Close]

BLAST of Cp4.1LG10g03120 vs. ExPASy TrEMBL
Match: A0A0A0KJ66 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950 PE=3 SV=1)

HSP 1 Score: 319 bits (818), Expect = 8.37e-110
Identity = 161/174 (92.53%), Postives = 166/174 (95.40%), Query Frame = 0


Query: 1   MAFSSITMKCP--PVLHLRALPKSSAPQSSISFRYSPTKFSRRKTLSIRSVSVPAAPASE 60
           MAFSSI+MKCP  PVLHL ALPKS APQ+SISFR+  TKFSRRK+LSIRSVSVPAAPASE
Sbjct: 1   MAFSSISMKCPCPPVLHLPALPKSYAPQTSISFRFPSTKFSRRKSLSIRSVSVPAAPASE 60

Query: 61  GIAPAISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSII 120
           GIAPAISLTD ALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFE+RANARPDDSII
Sbjct: 61  GIAPAISLTDTALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFESRANARPDDSII 120

Query: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFAAEM 172
           EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF AEM
Sbjct: 121 EYNGFVIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSFNAEM 174

[Close]

BLAST of Cp4.1LG10g03120 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 244.2 bits (622), Expect = 7.7e-65
Identity = 120/149 (80.54%), Postives = 130/149 (87.25%), Query Frame = 0


Query: 27  SSISF---RYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHLNKMRSERNE 86
           +SISF    Y     +RR  LS+RS SVPAAPA EG+ PAISL++NALKHL+KMRSER E
Sbjct: 32  NSISFPSLSYVNLNLNRRNRLSVRSASVPAAPAMEGLKPAISLSENALKHLSKMRSERGE 91

Query: 87  DLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLFLFGMQLDYS 146
           DLCLRIGV+QGGCSGMSYTM+FENRANARPDDS IEY GF IVCDPKS+LFLFGMQLDYS
Sbjct: 92  DLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGFTIVCDPKSMLFLFGMQLDYS 151

Query: 147 DALIGGGFSFKNPNATQTCGCGKSFAAEM 173
           DALIGGGFSF NPNATQTCGCGKSFAAEM
Sbjct: 152 DALIGGGFSFSNPNATQTCGCGKSFAAEM 180

[Close]

BLAST of Cp4.1LG10g03120 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.1e-15
Identity = 53/159 (33.33%), Postives = 75/159 (47.17%), Query Frame = 0


Query: 17  RALPKSSAP--QSSISFRYSPTKFSRRKTLSIRSVSVPAAPASEGIAPAISLTDNALKHL 76
           R+L K  AP     I   +    FS    +   S S  + P S      + L+DN ++ +
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSS-NDVVHLSDNCIRRM 62

Query: 77  NKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGFVIVCDPKSLLF 136
            +++S   E   LR+GV  GGCSG  Y  E +NR N  PDD + E NG  +V D  S   
Sbjct: 63  KELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTN--PDDRVFEKNGVKLVVDNVSYDL 122

Query: 137 LFGMQLDYSDALIGGGFSFK-NPNATQTCGCGKSFAAEM 173
           + G  +DY + LI   F    NP+A   C C  SF  ++
Sbjct: 123 VKGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMVKL 158

[Close]

BLAST of Cp4.1LG10g03120 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-12
Identity = 34/105 (32.38%), Postives = 60/105 (57.14%), Query Frame = 0


Query: 64  ISLTDNALKHLNKMRSERNEDLCLRIGVRQGGCSGMSYTMEFENRANARPDDSIIEYNGF 123
           ++L+D A   + ++   + +   LR+ V   GC+G+SY + +         D ++E  G 
Sbjct: 6   LALSDTAAARIRQLLQHQQKPF-LRLAVEAKGCNGLSYVLNYAQEKG--KFDEVVEEKGV 65

Query: 124 VIVCDPKSLLFLFGMQLDYSDALIGGGFSFKNPNATQTCGCGKSF 169
            I+ DPK+++ + G ++D+ D  +   F F NPNAT+ CGCG+SF
Sbjct: 66  KILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNATK-CGCGESF 106

The following BLAST results are available for this feature:

BLAST of Cp4.1LG10g03120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XIK3	1.1e-63	80.54	Iron-sulfur assembly protein IscA, chloroplastic OS=Arabidopsis thaliana OX=3702...	[more]
P72731	3.3e-36	62.96	Uncharacterized protein slr1417 OS=Synechocystis sp. (strain PCC 6803 / Kazusa) ...	[more]
P51217	3.7e-32	55.66	Uncharacterized protein ycf83 OS=Porphyra purpurea OX=2787 GN=ycf83 PE=3 SV=1	[more]
Q1XDR9	1.6e-30	54.46	Uncharacterized protein ycf83 OS=Pyropia yezoensis OX=2788 GN=ycf83 PE=3 SV=1	[more]
Q9MSA1	7.8e-30	54.21	Uncharacterized protein ycf83 OS=Galdieria sulphuraria OX=130081 GN=ycf83 PE=3 S...	[more]

BLAST of Cp4.1LG10g03120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022950286.1	2.40e-120	100.00	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita moschata] >XP_0...	[more]
KAG7034403.1	9.78e-120	99.42	Iron-sulfur assembly protein IscA, chloroplastic [Cucurbita argyrosperma subsp. ...	[more]
XP_022977954.1	2.31e-118	98.84	iron-sulfur assembly protein IscA, chloroplastic-like [Cucurbita maxima]	[more]
XP_038880838.1	1.10e-116	96.51	iron-sulfur assembly protein IscA, chloroplastic [Benincasa hispida]	[more]
XP_008440129.1	6.10e-114	95.93	PREDICTED: iron-sulfur assembly protein IscA, chloroplastic [Cucumis melo]	[more]

BLAST of Cp4.1LG10g03120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GEF0	1.16e-120	100.00	iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita moschata OX=3...	[more]
A0A6J1ISS5	1.12e-118	98.84	iron-sulfur assembly protein IscA, chloroplastic-like OS=Cucurbita maxima OX=366...	[more]
A0A1S3B105	2.95e-114	95.93	iron-sulfur assembly protein IscA, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A6J1BTR2	1.35e-110	93.02	iron-sulfur assembly protein IscA, chloroplastic isoform X1 OS=Momordica charant...	[more]
A0A0A0KJ66	8.37e-110	92.53	Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G486950...	[more]

BLAST of Cp4.1LG10g03120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G10500.1	7.7e-65	80.54	chloroplast-localized ISCA-like protein	[more]
AT5G03905.1	1.1e-15	33.33	Iron-sulphur cluster biosynthesis family protein	[more]
AT2G36260.1	1.5e-12	32.38	Iron-sulphur cluster biosynthesis family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016092	FeS cluster insertion protein	TIGRFAM	TIGR00049	TIGR00049	coord: 64..169 e-value: 2.8E-37 score: 125.1
IPR000361	FeS cluster biogenesis	PFAM	PF01521	Fe-S_biosyn	coord: 64..166 e-value: 4.5E-22 score: 78.3
IPR035903	HesB-like domain superfamily	GENE3D	2.60.300.12		coord: 63..171 e-value: 1.4E-35 score: 123.7
IPR035903	HesB-like domain superfamily	SUPERFAMILY	89360	HesB-like domain	coord: 63..159
None	No IPR available	PANTHER	PTHR47265:SF1	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 1..172
IPR031108	FeS assembly protein IscA, plant/cyanobacteria type	PANTHER	PTHR47265	IRON-SULFUR ASSEMBLY PROTEIN ISCA, CHLOROPLASTIC	coord: 1..172
IPR017870	FeS cluster insertion, C-terminal, conserved site	PROSITE	PS01152	HESB	coord: 151..168

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG10g03120.1	Cp4.1LG10g03120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0051536	iron-sulfur cluster binding
molecular_function	GO:0030674	protein-macromolecule adaptor activity