Cp4.1LG10g01970 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g01970
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description1-aminocyclopropane-1-carboxylate synthase
LocationCp4.1LG10: 2963038 .. 2964676 (+)
RNA-Seq ExpressionCp4.1LG10g01970
SyntenyCp4.1LG10g01970
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGGATGCTATCCACAAAGGCTACGTGCAACTCCCACGGTCAAGATTCCTCCTACTTCCTAGGGTGGGAGGCTTATGAGAAGAATCCCTTTGATGAGGCTTCTAATCCCAAGGGCATAATTCAGATGGGTCTTGCGGAGAATCAAGTAATTGATTGTTCATATTCATCCATTTTGGCTACCTTTTTTATGCCTCAATTATTCACCTTTTCTTCTTCCTCTCATTTTGCAGCTATCATTTGACCTTCTTGAATCATGGCTCACAAAGAACCCTGACGCCGCCAGCTTCAAACGAGATGGCCAATCCATTTTCAGGGAGCTCGCTCTCTTCCAAGATTACCACGGCTTACCCGCATTCAAAAAGGCATTGGTTGAATTTATGGCGGAAATTAGAGGGAACAAAGTAACATTTGAGGCTAATAACATAGTCCTCACAGCTGGCGCAACTTCAGCCAATGAGACACTTATGTTCTGCCTTGCCGAGGCTGGCGATGCTTTTCTCCTCCCAACTCCATACTATCCAGGGTTCGTATTCCTATAAATAATAATAATAATGATAATCTTTACTAAAATTTGAAGCTATATTATGAAGTTATTAAGAATCATAATTCTGATCTTTACTTGCATTTGTTGTACAAACAGATTTGATAGAGATTTGAAATGGAGAACCGGCGTTGAGATTGTACCAATTCATTGCACCAGCTCCAACGCCTTCCAAGTCACCCAACCGGCTCTAGAACAAGCCTATAAAGAAGCCGAAGCTCGCAACCTACGTGTCAAAGGCGTGTTGGTCACAAACCCATCCAACCCATTGGGCACCACAATGAGCCGACACGAACTCGACTTGGTTTTCGACTTTATAACTTCGAAAGGCATTCACTTGATCAGTGACGAGATCTACTCTGGGACCGTTTTCGGGTCGCCGGGATTCGTGAGTGCGATGGAGGTGCTTAGGGAGAGGAGTAGCGAGGAGGAGGAGGTTTGGAAGAGAGTACATATTGTTTACAGTTTGTCAAAGGATTTGGGGCTTCCAGGTTTTCGAGTTGGTGCAATTTACTCCAACGACGACATGGTGGTGGCTGCGGCTACCAAAATGTCTAGCTTTGGATTGGTGTCGTCTCAAACACAATACCTTCTCTCAGCCATGCTGTCCGACAAGAAGTTCACTAGAAACTACATTACCGAGAATCAGAGAAGGCTGAAACAGAGACAAAAGATGCTGGTATCTGGTTTGGAAAAGGCGGGTATTAACTGCCTGGACAGCAATGCAGGGTTGTTCTGTTGGGTGGACATGAGGCATTTGTTGGAGTCGGACACGTTTGAATCCGAGTTAGAGCTGTGGAAGAAGATAGTGTACGAGGTGGGTTTGAACATTTCTCCTGGCTCATCCTGCCACTGCTCTGAACCAGGGTGGTTCCGTGTTTGCTTTGCCAACATGTCCGAATCAACATTGAAACTAGCTATTCGAAGATTGAAGTCGTTTGTAGCAGAATTGAGGTCCGCCGCTTCTCCAAACGCTCAGTCACAATTAAACAATGACAACAAATTTTGCAAGAACATCAAGAAAAACATTTTCACCAAATGGGTTTTCCGGCTATCGGTTCAAGAAGCCAACCGCAAGCTGCAACATGAACGATAG

mRNA sequence

ATGAGGATGCTATCCACAAAGGCTACGTGCAACTCCCACGGTCAAGATTCCTCCTACTTCCTAGGGTGGGAGGCTTATGAGAAGAATCCCTTTGATGAGGCTTCTAATCCCAAGGGCATAATTCAGATGGGTCTTGCGGAGAATCAACTATCATTTGACCTTCTTGAATCATGGCTCACAAAGAACCCTGACGCCGCCAGCTTCAAACGAGATGGCCAATCCATTTTCAGGGAGCTCGCTCTCTTCCAAGATTACCACGGCTTACCCGCATTCAAAAAGGCATTGGTTGAATTTATGGCGGAAATTAGAGGGAACAAAGTAACATTTGAGGCTAATAACATAGTCCTCACAGCTGGCGCAACTTCAGCCAATGAGACACTTATGTTCTGCCTTGCCGAGGCTGGCGATGCTTTTCTCCTCCCAACTCCATACTATCCAGGATTTGATAGAGATTTGAAATGGAGAACCGGCGTTGAGATTGTACCAATTCATTGCACCAGCTCCAACGCCTTCCAAGTCACCCAACCGGCTCTAGAACAAGCCTATAAAGAAGCCGAAGCTCGCAACCTACGTGTCAAAGGCGTGTTGGTCACAAACCCATCCAACCCATTGGGCACCACAATGAGCCGACACGAACTCGACTTGGTTTTCGACTTTATAACTTCGAAAGGCATTCACTTGATCAGTGACGAGATCTACTCTGGGACCGTTTTCGGGTCGCCGGGATTCGTGAGTGCGATGGAGGTGCTTAGGGAGAGGAGTAGCGAGGAGGAGGAGGTTTGGAAGAGAGTACATATTGTTTACAGTTTGTCAAAGGATTTGGGGCTTCCAGGTTTTCGAGTTGGTGCAATTTACTCCAACGACGACATGGTGGTGGCTGCGGCTACCAAAATGTCTAGCTTTGGATTGGTGTCGTCTCAAACACAATACCTTCTCTCAGCCATGCTGTCCGACAAGAAGTTCACTAGAAACTACATTACCGAGAATCAGAGAAGGCTGAAACAGAGACAAAAGATGCTGGTATCTGGTTTGGAAAAGGCGGGTATTAACTGCCTGGACAGCAATGCAGGGTTGTTCTGTTGGGTGGACATGAGGCATTTGTTGGAGTCGGACACGTTTGAATCCGAGTTAGAGCTGTGGAAGAAGATAGTGTACGAGGTGGGTTTGAACATTTCTCCTGGCTCATCCTGCCACTGCTCTGAACCAGGGTGGTTCCGTGTTTGCTTTGCCAACATGTCCGAATCAACATTGAAACTAGCTATTCGAAGATTGAAGTCGTTTGTAGCAGAATTGAGGTCCGCCGCTTCTCCAAACGCTCAGTCACAATTAAACAATGACAACAAATTTTGCAAGAACATCAAGAAAAACATTTTCACCAAATGGGTTTTCCGGCTATCGGTTCAAGAAGCCAACCGCAAGCTGCAACATGAACGATAG

Coding sequence (CDS)

ATGAGGATGCTATCCACAAAGGCTACGTGCAACTCCCACGGTCAAGATTCCTCCTACTTCCTAGGGTGGGAGGCTTATGAGAAGAATCCCTTTGATGAGGCTTCTAATCCCAAGGGCATAATTCAGATGGGTCTTGCGGAGAATCAACTATCATTTGACCTTCTTGAATCATGGCTCACAAAGAACCCTGACGCCGCCAGCTTCAAACGAGATGGCCAATCCATTTTCAGGGAGCTCGCTCTCTTCCAAGATTACCACGGCTTACCCGCATTCAAAAAGGCATTGGTTGAATTTATGGCGGAAATTAGAGGGAACAAAGTAACATTTGAGGCTAATAACATAGTCCTCACAGCTGGCGCAACTTCAGCCAATGAGACACTTATGTTCTGCCTTGCCGAGGCTGGCGATGCTTTTCTCCTCCCAACTCCATACTATCCAGGATTTGATAGAGATTTGAAATGGAGAACCGGCGTTGAGATTGTACCAATTCATTGCACCAGCTCCAACGCCTTCCAAGTCACCCAACCGGCTCTAGAACAAGCCTATAAAGAAGCCGAAGCTCGCAACCTACGTGTCAAAGGCGTGTTGGTCACAAACCCATCCAACCCATTGGGCACCACAATGAGCCGACACGAACTCGACTTGGTTTTCGACTTTATAACTTCGAAAGGCATTCACTTGATCAGTGACGAGATCTACTCTGGGACCGTTTTCGGGTCGCCGGGATTCGTGAGTGCGATGGAGGTGCTTAGGGAGAGGAGTAGCGAGGAGGAGGAGGTTTGGAAGAGAGTACATATTGTTTACAGTTTGTCAAAGGATTTGGGGCTTCCAGGTTTTCGAGTTGGTGCAATTTACTCCAACGACGACATGGTGGTGGCTGCGGCTACCAAAATGTCTAGCTTTGGATTGGTGTCGTCTCAAACACAATACCTTCTCTCAGCCATGCTGTCCGACAAGAAGTTCACTAGAAACTACATTACCGAGAATCAGAGAAGGCTGAAACAGAGACAAAAGATGCTGGTATCTGGTTTGGAAAAGGCGGGTATTAACTGCCTGGACAGCAATGCAGGGTTGTTCTGTTGGGTGGACATGAGGCATTTGTTGGAGTCGGACACGTTTGAATCCGAGTTAGAGCTGTGGAAGAAGATAGTGTACGAGGTGGGTTTGAACATTTCTCCTGGCTCATCCTGCCACTGCTCTGAACCAGGGTGGTTCCGTGTTTGCTTTGCCAACATGTCCGAATCAACATTGAAACTAGCTATTCGAAGATTGAAGTCGTTTGTAGCAGAATTGAGGTCCGCCGCTTCTCCAAACGCTCAGTCACAATTAAACAATGACAACAAATTTTGCAAGAACATCAAGAAAAACATTTTCACCAAATGGGTTTTCCGGCTATCGGTTCAAGAAGCCAACCGCAAGCTGCAACATGAACGATAG

Protein sequence

MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLTKNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGATSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQAYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGSPGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSSFGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFCWVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLAIRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER
Homology
BLAST of Cp4.1LG10g01970 vs. ExPASy Swiss-Prot
Match: Q00257 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN=ACS2 PE=2 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 8.2e-255
Identity = 434/478 (90.79%), Postives = 455/478 (95.19%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYE NPF   SNP GIIQMGLAENQLSFDLLESWL+
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYENNPFHHTSNPNGIIQMGLAENQLSFDLLESWLS 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKV+FEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVSFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQ+TQ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITQSALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYK+A+ RNLRVKGVLVTNPSNPLGTTM+R EL+LVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKDAQTRNLRVKGVLVTNPSNPLGTTMNRDELNLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVL+ERSSE+EEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFT +YI+ENQ+RLKQRQKMLVSGL+KAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTISYISENQKRLKQRQKMLVSGLQKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESD FESELELWKKIVYEVGLNISPGSSCHC+EPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDKFESELELWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 479
           +RRLKSFV ELRS  + N +   N+DNK CKNIKKNIFTKWVFR S QEANRK+Q ER
Sbjct: 421 VRRLKSFVTELRSTTTSNHR---NHDNKICKNIKKNIFTKWVFRQSAQEANRKMQAER 475

BLAST of Cp4.1LG10g01970 vs. ExPASy Swiss-Prot
Match: Q42881 (1-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=ACS3 PE=1 SV=1)

HSP 1 Score: 746.9 bits (1927), Expect = 1.4e-214
Identity = 357/473 (75.48%), Postives = 414/473 (87.53%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M++LS KATCNSHGQDSSYFLGW+ YEKNP+DE  NPKGIIQMGLAENQLSFDLLESWL 
Sbjct: 1   MKLLSEKATCNSHGQDSSYFLGWQEYEKNPYDEIQNPKGIIQMGLAENQLSFDLLESWLA 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           +NPDAA FKR+G+SIFRELALFQDYHGLPAFK A+ +FM+EIRGN+V+F++NN+VLTAGA
Sbjct: 61  QNPDAAGFKRNGESIFRELALFQDYHGLPAFKNAMTKFMSEIRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLA  GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN F++T+ ALE+
Sbjct: 121 TSANETLMFCLANQGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFRITESALEE 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY +A+ RNL+VKGVLVTNPSNPLGTT++R+EL+L+  FI  KGIHLISDEIYSGTVF S
Sbjct: 181 AYLDAKKRNLKVKGVLVTNPSNPLGTTLNRNELELLLTFIDEKGIHLISDEIYSGTVFNS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVS MEVL E++  +  VW+RVHIVYSLSKDLGLPGFR+GAIYSND+MVV+AATKMSS
Sbjct: 241 PGFVSVMEVLIEKNYMKTRVWERVHIVYSLSKDLGLPGFRIGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLS MLSDKKFT+ YI+ENQ+RLK+R  MLV GL+ AGINCL+SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSCMLSDKKFTKKYISENQKRLKKRHAMLVKGLKSAGINCLESNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL S+ F++E++LWKKIVY+VGLNISPGSSCHC+EPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLSSNNFDAEMDLWKKIVYDVGLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRK 474
           +RR+K FV     + +PNA +   N  +   N KK  F+KWVFRLS  +  R+
Sbjct: 421 MRRIKDFV----ESTAPNATNH-QNQQQSNANSKKKSFSKWVFRLSFNDRQRE 468

BLAST of Cp4.1LG10g01970 vs. ExPASy Swiss-Prot
Match: P37821 (1-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 PE=1 SV=2)

HSP 1 Score: 728.4 bits (1879), Expect = 5.2e-209
Identity = 353/470 (75.11%), Postives = 405/470 (86.17%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           MRMLS  AT NSHGQDSSYFLGW+ YEKNP+ E  N  GIIQMGLAENQL FDLLESWL 
Sbjct: 1   MRMLSRNATFNSHGQDSSYFLGWQEYEKNPYHEVHNTNGIIQMGLAENQLCFDLLESWLA 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNP+AA+FK++G+SIF ELALFQDYHGLPAFKKA+V+FMAEIRGNKVTF+ N++VLTAGA
Sbjct: 61  KNPEAAAFKKNGESIFAELALFQDYHGLPAFKKAMVDFMAEIRGNKVTFDPNHLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANET +FCLA+ G+A L+PTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQ+T+ ALE+
Sbjct: 121 TSANETFIFCLADPGEAVLIPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQITETALEE 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+EAE RNLRVKGVLVTNPSNPLGTTM+R+EL L+  F+  KGIHLISDEIYSGT F S
Sbjct: 181 AYQEAEKRNLRVKGVLVTNPSNPLGTTMTRNELYLLLSFVEDKGIHLISDEIYSGTAFSS 240

Query: 241 PGFVSAMEVLRERSSEE-EEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300
           P F+S MEVL++R+ +E  EVW+RVH+VYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS
Sbjct: 241 PSFISVMEVLKDRNCDENSEVWQRVHVVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLF 360
           SFGLVSSQTQ+LLSAMLSDKK T+NYI EN +RLKQRQK LVSGL+K+GI+CL+ NAGLF
Sbjct: 301 SFGLVSSQTQHLLSAMLSDKKLTKNYIAENHKRLKQRQKKLVSGLQKSGISCLNGNAGLF 360

Query: 361 CWVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKL 420
           CWVDMRHLL S+TFE+E+ELWKKIVYEV LNISPGSSCHC+EPGWFRVCFAN+ E TL L
Sbjct: 361 CWVDMRHLLRSNTFEAEMELWKKIVYEVHLNISPGSSCHCTEPGWFRVCFANLPERTLDL 420

Query: 421 AIRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQE 470
           A++RLK+FV E  +    N  SQ    +    + ++   TKWV RLS  +
Sbjct: 421 AMQRLKAFVGEYYNVPEVNGGSQ----SSHLSHSRRQSLTKWVSRLSFDD 466

BLAST of Cp4.1LG10g01970 vs. ExPASy Swiss-Prot
Match: Q9M2Y8 (1-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=ACS9 PE=1 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 1.7e-204
Identity = 348/466 (74.68%), Postives = 399/466 (85.62%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+ LS K T N+HGQDSSYFLGWE YEKNP+DE  NP GIIQMGLAENQL FDL+E+WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAA  K+DGQSIF+ELALFQDYHGLP FKKAL EFM EIRGN+VTF+ + IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQ+T+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY++A+  +L+VKGVLVTNPSNPLGT ++R EL+L+ DFITSK IHLISDEIYSGTVFG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             FVS M+VL++++ E  EV KRVHIVYSLSKDLGLPGFRVGAIYSND+MVV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT  Y+ ENQ+RLK RQK LVSGLE AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+ELELWKKIVY+V LNISPGSSCHC+EPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLS 467
           ++RLK +V    S  S    S+ ++D    K+++K   + WVFR+S
Sbjct: 421 MKRLKEYV---ESTDSRRVISKSSHDR--IKSLRKRTVSNWVFRVS 461

BLAST of Cp4.1LG10g01970 vs. ExPASy Swiss-Prot
Match: Q37001 (1-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=ACS5 PE=1 SV=1)

HSP 1 Score: 712.2 bits (1837), Expect = 3.9e-204
Identity = 342/466 (73.39%), Postives = 397/466 (85.19%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+ LSTK T N HGQDSSYFLGWE YEKNP+DE  NP G+IQMGLAENQL FDL+ESWLT
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAAS KR+GQSIFRELALFQDYHG+P FKKA+ EFM EIRGN+VTF+   IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQ+T+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY++A+  +L+VKGVLVTNPSNPLGT ++R EL+L+ DFITSK IHLISDEIYSGT+FG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             F+S M+VL+++  E+ EV KRVH+VYSLSKDLGLPGFRVGAIYSND+M+V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT  Y+ ENQ+RLK RQ+ LVSGLE AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+EL+LWKKIVY V LNISPGSSCHC+EPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLS 467
           ++RLK+FV         +  S     ++  K+++K   + WVFR+S
Sbjct: 421 LKRLKTFVESTDCGRMISRSS-----HERLKSLRKKTVSNWVFRVS 461

BLAST of Cp4.1LG10g01970 vs. NCBI nr
Match: XP_023544912.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 957 bits (2475), Expect = 0.0
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478

BLAST of Cp4.1LG10g01970 vs. NCBI nr
Match: XP_022950799.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita moschata])

HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 474/478 (99.16%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVT+PALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTRPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFR+CFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRLCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478

BLAST of Cp4.1LG10g01970 vs. NCBI nr
Match: KAG7034265.1 (1-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 952 bits (2460), Expect = 0.0
Identity = 473/478 (98.95%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 4   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 63

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 64  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 123

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVT+PALEQ
Sbjct: 124 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTRPALEQ 183

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 184 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 243

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 244 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 303

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 304 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 363

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFR+CFANMSESTLKLA
Sbjct: 364 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRLCFANMSESTLKLA 423

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKN+KKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 424 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNVKKNIFTKWVFRLSVQEANRKLQHER 481

BLAST of Cp4.1LG10g01970 vs. NCBI nr
Match: KAG6604101.1 (1-aminocyclopropane-1-carboxylate synthase 101, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 951 bits (2457), Expect = 0.0
Identity = 473/478 (98.95%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVT+PALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTRPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSS+EEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSKEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFR+CFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRLCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478

BLAST of Cp4.1LG10g01970 vs. NCBI nr
Match: XP_022978599.1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita maxima])

HSP 1 Score: 947 bits (2448), Expect = 0.0
Identity = 470/478 (98.33%), Postives = 476/478 (99.58%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVTQPALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTQPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFG 
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGF 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSS+EEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSQEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYI+ENQ+RLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYISENQKRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCS+PGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSQPGWFRVCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKN+KKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNVKKNIFTKWVFRLSVQEANRKLQHER 478

BLAST of Cp4.1LG10g01970 vs. ExPASy TrEMBL
Match: A0A6J1GGW6 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita moschata OX=3662 GN=LOC111453796 PE=3 SV=1)

HSP 1 Score: 952 bits (2461), Expect = 0.0
Identity = 474/478 (99.16%), Postives = 477/478 (99.79%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVT+PALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTRPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFR+CFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRLCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478

BLAST of Cp4.1LG10g01970 vs. ExPASy TrEMBL
Match: A0A6J1IUH1 (1-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita maxima OX=3661 GN=LOC111478535 PE=3 SV=1)

HSP 1 Score: 947 bits (2448), Expect = 0.0
Identity = 470/478 (98.33%), Postives = 476/478 (99.58%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVTQPALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTQPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFG 
Sbjct: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGF 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSS+EEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSQEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYI+ENQ+RLKQRQKMLVSGLEKAGINCLDSNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYISENQKRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCS+PGWFRVCFANMSESTLKLA
Sbjct: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSQPGWFRVCFANMSESTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFVAELRSAASPNAQSQLNNDNKFCKN+KKNIFTKWVFRLSVQEANRKLQHER
Sbjct: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNVKKNIFTKWVFRLSVQEANRKLQHER 478

BLAST of Cp4.1LG10g01970 vs. ExPASy TrEMBL
Match: I1VWI5 (1-aminocyclopropane-1-carboxylate synthase OS=Citrullus lanatus subsp. vulgaris OX=260674 GN=ACS1 PE=2 SV=1)

HSP 1 Score: 896 bits (2315), Expect = 0.0
Identity = 446/478 (93.31%), Postives = 460/478 (96.23%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDE  NPKGIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDETCNPKGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVT+ ALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTKSALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEA+ RNLRVKGVLVTNPSNPLGTTM+RHELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAQTRNLRVKGVLVTNPSNPLGTTMTRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVLRERSSE+EEVWKRVHIVYSLSKDLGLPGFRVGAIYSND MVVAAATKMSS
Sbjct: 241 PGFVSAMEVLRERSSEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDQMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTRNYI+ENQ+RLKQRQ+MLVSGL KAGI CL+SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRNYISENQKRLKQRQQMLVSGLGKAGIKCLESNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESEL+LWKKIVYEVGLNISPGSSCHC+EPGWFRVCFANMS+STLKLA
Sbjct: 361 WVDMRHLLESDTFESELKLWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSQSTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQEANRKLQHER 478
           IRRLKSFV ELRS  +PN  S  N DNKFCKNIKKNIFTKWVFR SVQEANRKL HER
Sbjct: 421 IRRLKSFVRELRSLNTPNVVST-NYDNKFCKNIKKNIFTKWVFRQSVQEANRKLHHER 477

BLAST of Cp4.1LG10g01970 vs. ExPASy TrEMBL
Match: A0A5A7SZ75 (1-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G003750 PE=3 SV=1)

HSP 1 Score: 887 bits (2292), Expect = 0.0
Identity = 443/481 (92.10%), Postives = 462/481 (96.05%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDE SNP GIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDETSNPNGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVTQPALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTQPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AYKEAE+RNLRVKGVLVTNPSNPLGTTM+R+ELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYKEAESRNLRVKGVLVTNPSNPLGTTMTRNELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVL+ERS+EEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSND+MVVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSNEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTR YI+ENQ+RLKQRQKMLVSGLEKAGI CL+SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRTYISENQKRLKQRQKMLVSGLEKAGIKCLESNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFESEL+LWKKIVYEVGLNISPGSSCHC+EPGWFRVCFANMS+STLKLA
Sbjct: 361 WVDMRHLLESDTFESELKLWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSQSTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNN--DNKFCKNIKKNIFTKWVFRLSVQE-ANRKLQHE 478
           IRRLKSFV ELRS ++ N  +   N  DNKF KNIKKNIFTKWVFR SVQ+ ANRKL +E
Sbjct: 421 IRRLKSFVQELRSVSTANVSTTATNIHDNKFSKNIKKNIFTKWVFRQSVQDQANRKLHYE 480

BLAST of Cp4.1LG10g01970 vs. ExPASy TrEMBL
Match: Q09PK3 (1-aminocyclopropane-1-carboxylate synthase OS=Cucumis sativus OX=3659 GN=ACS1 PE=3 SV=1)

HSP 1 Score: 884 bits (2283), Expect = 0.0
Identity = 440/481 (91.48%), Postives = 462/481 (96.05%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+MLSTKATCNSHGQDSSYFLGWEAYEKNPFDE SNP GIIQMGLAENQLSFDLLESWLT
Sbjct: 1   MKMLSTKATCNSHGQDSSYFLGWEAYEKNPFDETSNPNGIIQMGLAENQLSFDLLESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAASFKRDG+SIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA
Sbjct: 61  KNPDAASFKRDGKSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSN FQVTQPALEQ
Sbjct: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNGFQVTQPALEQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY+EA+ARNLRVKGVLVTNPSNPLGTTM+R+ELDLVFDFITSKGIHLISDEIYSGTVFGS
Sbjct: 181 AYQEAQARNLRVKGVLVTNPSNPLGTTMTRNELDLVFDFITSKGIHLISDEIYSGTVFGS 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGFVSAMEVL+ERS+E+EEVWKRVHIVYSLSKDLGLPGFRVGAIYSND+MVVAAATKMSS
Sbjct: 241 PGFVSAMEVLKERSNEDEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVAAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSAMLSDKKFTR YI+ENQ+RLKQRQKMLVSGLEKAGI CL+SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSAMLSDKKFTRTYISENQKRLKQRQKMLVSGLEKAGIKCLESNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLLESDTFE EL+LWKKIVYEVGLNISPGSSCHC+EPGWFRVCFANMS+STLKLA
Sbjct: 361 WVDMRHLLESDTFECELKLWKKIVYEVGLNISPGSSCHCTEPGWFRVCFANMSQSTLKLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNN--DNKFCKNIKKNIFTKWVFRLSVQE-ANRKLQHE 478
           IRRLKSFV ELRS ++PN  +   N  D+KF KNIKKNIFTKWVFR SVQ+ ANRKL +E
Sbjct: 421 IRRLKSFVQELRSVSTPNVSTTTTNIHDSKFSKNIKKNIFTKWVFRQSVQDQANRKLHYE 480

BLAST of Cp4.1LG10g01970 vs. TAIR 10
Match: AT3G49700.1 (1-aminocyclopropane-1-carboxylate synthase 9 )

HSP 1 Score: 713.4 bits (1840), Expect = 1.2e-205
Identity = 348/466 (74.68%), Postives = 399/466 (85.62%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+ LS K T N+HGQDSSYFLGWE YEKNP+DE  NP GIIQMGLAENQL FDL+E+WL 
Sbjct: 1   MKQLSRKVTSNAHGQDSSYFLGWEEYEKNPYDEIKNPNGIIQMGLAENQLCFDLIETWLA 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAA  K+DGQSIF+ELALFQDYHGLP FKKAL EFM EIRGN+VTF+ + IVL AG+
Sbjct: 61  KNPDAAGLKKDGQSIFKELALFQDYHGLPEFKKALAEFMEEIRGNRVTFDPSKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQ+T+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY++A+  +L+VKGVLVTNPSNPLGT ++R EL+L+ DFITSK IHLISDEIYSGTVFG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTMLTRRELNLLVDFITSKNIHLISDEIYSGTVFGF 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             FVS M+VL++++ E  EV KRVHIVYSLSKDLGLPGFRVGAIYSND+MVV+AATKMSS
Sbjct: 241 EQFVSVMDVLKDKNLENSEVSKRVHIVYSLSKDLGLPGFRVGAIYSNDEMVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT  Y+ ENQ+RLK RQK LVSGLE AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSTYLDENQKRLKIRQKKLVSGLEAAGITCLKSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+ELELWKKIVY+V LNISPGSSCHC+EPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELELWKKIVYDVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLS 467
           ++RLK +V    S  S    S+ ++D    K+++K   + WVFR+S
Sbjct: 421 MKRLKEYV---ESTDSRRVISKSSHDR--IKSLRKRTVSNWVFRVS 461

BLAST of Cp4.1LG10g01970 vs. TAIR 10
Match: AT5G65800.1 (ACC synthase 5 )

HSP 1 Score: 712.2 bits (1837), Expect = 2.8e-205
Identity = 342/466 (73.39%), Postives = 397/466 (85.19%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M+ LSTK T N HGQDSSYFLGWE YEKNP+DE  NP G+IQMGLAENQL FDL+ESWLT
Sbjct: 1   MKQLSTKVTSNGHGQDSSYFLGWEEYEKNPYDEIKNPNGMIQMGLAENQLCFDLIESWLT 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAAS KR+GQSIFRELALFQDYHG+P FKKA+ EFM EIRGN+VTF+   IVL AG+
Sbjct: 61  KNPDAASLKRNGQSIFRELALFQDYHGMPEFKKAMAEFMEEIRGNRVTFDPKKIVLAAGS 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLAE GDAFLLPTPYYPGFDRDLKWRTG EIVPIHC+SSN FQ+T+ AL+Q
Sbjct: 121 TSANETLMFCLAEPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIHCSSSNGFQITESALQQ 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY++A+  +L+VKGVLVTNPSNPLGT ++R EL+L+ DFITSK IHLISDEIYSGT+FG 
Sbjct: 181 AYQQAQKLDLKVKGVLVTNPSNPLGTALTRRELNLLVDFITSKNIHLISDEIYSGTMFGF 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
             F+S M+VL+++  E+ EV KRVH+VYSLSKDLGLPGFRVGAIYSND+M+V+AATKMSS
Sbjct: 241 EQFISVMDVLKDKKLEDTEVSKRVHVVYSLSKDLGLPGFRVGAIYSNDEMIVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGLVSSQTQYLLSA+LSDKKFT  Y+ ENQ+RLK RQ+ LVSGLE AGI CL SNAGLFC
Sbjct: 301 FGLVSSQTQYLLSALLSDKKFTSQYLEENQKRLKSRQRRLVSGLESAGITCLRSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+++TFE+EL+LWKKIVY V LNISPGSSCHC+EPGWFRVCFANMSE TL LA
Sbjct: 361 WVDMRHLLDTNTFEAELDLWKKIVYNVKLNISPGSSCHCTEPGWFRVCFANMSEDTLDLA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLS 467
           ++RLK+FV         +  S     ++  K+++K   + WVFR+S
Sbjct: 421 LKRLKTFVESTDCGRMISRSS-----HERLKSLRKKTVSNWVFRVS 461

BLAST of Cp4.1LG10g01970 vs. TAIR 10
Match: AT4G37770.1 (1-amino-cyclopropane-1-carboxylate synthase 8 )

HSP 1 Score: 706.4 bits (1822), Expect = 1.5e-203
Identity = 341/469 (72.71%), Postives = 399/469 (85.07%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M +LS KA+CN+HGQDSSYF GWE YEKNP+DE  NP GIIQMGLAENQLSFDL+ESWL 
Sbjct: 1   MGLLSKKASCNTHGQDSSYFWGWEEYEKNPYDEIKNPDGIIQMGLAENQLSFDLIESWLA 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           KNPDAA+F+R+GQSIFRELALFQDYHGLP+FK A+ +FM+E RGN+V+F  N +VLTAGA
Sbjct: 61  KNPDAANFQREGQSIFRELALFQDYHGLPSFKNAMADFMSENRGNRVSFNPNKLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           T ANETLMFCLA+ GDAFLLPTPYYPGFDRDLKWRTG EIVPI C S+N F++T+ ALE+
Sbjct: 121 TPANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGAEIVPIQCKSANGFRITKVALEE 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGS 240
           AY++A+  NL+VKGVL+TNPSNPLGTT +R EL+ + DFI+ K IHLISDEIYSGTVF +
Sbjct: 181 AYEQAQKLNLKVKGVLITNPSNPLGTTTTRTELNHLLDFISRKKIHLISDEIYSGTVFTN 240

Query: 241 PGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSS 300
           PGF+S MEVL++R  E  +V+ RVHIVYSLSKDLGLPGFRVG IYSNDD VV+AATKMSS
Sbjct: 241 PGFISVMEVLKDRKLENTDVFDRVHIVYSLSKDLGLPGFRVGVIYSNDDFVVSAATKMSS 300

Query: 301 FGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFC 360
           FGL+SSQTQYLLSA+LSDK FT+NY+ ENQ RLK R K LVSGLE AGI CL SNAGLFC
Sbjct: 301 FGLISSQTQYLLSALLSDKTFTKNYLEENQIRLKNRHKKLVSGLEAAGIECLKSNAGLFC 360

Query: 361 WVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLA 420
           WVDMRHLL+S+TFE+E+ELWKKIVYEV LNISPGSSCHC+EPGWFRVCFAN+SE TLK+A
Sbjct: 361 WVDMRHLLKSNTFEAEIELWKKIVYEVKLNISPGSSCHCNEPGWFRVCFANLSEETLKVA 420

Query: 421 IRRLKSFVAELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQE 470
           + RLK FV       SP  +SQ  ++++  KN++K   + WVFRLS  +
Sbjct: 421 LDRLKRFV----DGPSPTRRSQ--SEHQRLKNLRKMKVSNWVFRLSFHD 463

BLAST of Cp4.1LG10g01970 vs. TAIR 10
Match: AT2G22810.1 (1-aminocyclopropane-1-carboxylate synthase 4 )

HSP 1 Score: 667.2 bits (1720), Expect = 1.0e-191
Identity = 330/471 (70.06%), Postives = 388/471 (82.38%), Query Frame = 0

Query: 1   MRMLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLT 60
           M  LS KATCNSHGQ SSYFLGWE YEKNP+D   NP+GIIQMGLAENQL FDLLESWL 
Sbjct: 1   MVQLSRKATCNSHGQVSSYFLGWEEYEKNPYDVTKNPQGIIQMGLAENQLCFDLLESWLA 60

Query: 61  KNPDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGA 120
           +N DAA FKRDGQS+FRELALFQDYHGL +FK A  +FM+E RGN+V+F++NN+VLTAGA
Sbjct: 61  QNTDAACFKRDGQSVFRELALFQDYHGLSSFKNAFADFMSENRGNRVSFDSNNLVLTAGA 120

Query: 121 TSANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQ 180
           TSANETLMFCLA+ GDAFLLPTPYYPGFDRDLKWRTGVEIVPI  +S+N F++T+ ALE+
Sbjct: 121 TSANETLMFCLADPGDAFLLPTPYYPGFDRDLKWRTGVEIVPIQSSSTNGFRITKLALEE 180

Query: 181 AYKEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFIT-SKGIHLISDEIYSGTVFG 240
           AY++A+  +L VKG+L+TNPSNPLGTT ++ EL+++FDFIT +K IHL+SDEIYSGTVF 
Sbjct: 181 AYEQAKKLDLNVKGILITNPSNPLGTTTTQTELNILFDFITKNKNIHLVSDEIYSGTVFN 240

Query: 241 SPGFVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMS 300
           S  F+S ME+L+    E  +V  RVHIV SLSKDLGLPGFRVGAIYSND  V++AATKMS
Sbjct: 241 SSEFISVMEILKNNQLENTDVLNRVHIVCSLSKDLGLPGFRVGAIYSNDKDVISAATKMS 300

Query: 301 SFGLVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLF 360
           SFGLVSSQTQYLLS++LSDKKFT+NY+ ENQ+RLK RQ+ LV GLE  GI CL SNAGLF
Sbjct: 301 SFGLVSSQTQYLLSSLLSDKKFTKNYLRENQKRLKNRQRKLVLGLEAIGIKCLKSNAGLF 360

Query: 361 CWVDMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKL 420
           CWVDMR LL S TFE+E++LWKKIVYEV LNISPGSSCHC EPGWFRVCFANM + TLKL
Sbjct: 361 CWVDMRPLLRSKTFEAEMDLWKKIVYEVKLNISPGSSCHCEEPGWFRVCFANMIDETLKL 420

Query: 421 AIRRLKSFV-AELRSAASPNAQSQLNNDNKFCKNIKKNIFTKWVFRLSVQE 470
           A++RLK  V  E  S     ++S+  N ++  K    N+ + WVFRLS  +
Sbjct: 421 ALKRLKMLVDDENSSRRCQKSKSERLNGSR--KKTMSNV-SNWVFRLSFHD 468

BLAST of Cp4.1LG10g01970 vs. TAIR 10
Match: AT4G08040.1 (1-aminocyclopropane-1-carboxylate synthase 11 )

HSP 1 Score: 617.8 bits (1592), Expect = 7.1e-177
Identity = 295/468 (63.03%), Postives = 373/468 (79.70%), Query Frame = 0

Query: 3   MLSTKATCNSHGQDSSYFLGWEAYEKNPFDEASNPKGIIQMGLAENQLSFDLLESWLTKN 62
           MLS+K   +SHGQDSSYFLGW+ YEKNPF E+ N  GI+QMGLAENQLSFDL+E WL ++
Sbjct: 1   MLSSKVVGDSHGQDSSYFLGWQEYEKNPFHESFNTSGIVQMGLAENQLSFDLIEKWLEEH 60

Query: 63  PDAASFKRDGQSIFRELALFQDYHGLPAFKKALVEFMAEIRGNKVTFEANNIVLTAGATS 122
           P+    K++ +S+FR+LALFQDYHGLPAFK A+ +FM +IR NKV F+ N +VLTAG+TS
Sbjct: 61  PEVLGLKKNDESVFRQLALFQDYHGLPAFKDAMAKFMGKIRENKVKFDTNKMVLTAGSTS 120

Query: 123 ANETLMFCLAEAGDAFLLPTPYYPGFDRDLKWRTGVEIVPIHCTSSNAFQVTQPALEQAY 182
           ANETLMFCLA  GDAFL+P PYYPGFDRDLKWRTGVEIVPIHC SSN +++T+ ALE AY
Sbjct: 121 ANETLMFCLANPGDAFLIPAPYYPGFDRDLKWRTGVEIVPIHCVSSNGYKITEDALEDAY 180

Query: 183 KEAEARNLRVKGVLVTNPSNPLGTTMSRHELDLVFDFITSKGIHLISDEIYSGTVFGSPG 242
           + A   NL VKGVL+TNPSNPLGT+ +R ELDL+  F ++K IH++SDEIYSGTVF SP 
Sbjct: 181 ERALKHNLNVKGVLITNPSNPLGTSTTREELDLLLTFTSTKKIHMVSDEIYSGTVFDSPE 240

Query: 243 FVSAMEVLRERSSEEEEVWKRVHIVYSLSKDLGLPGFRVGAIYSNDDMVVAAATKMSSFG 302
           F S +EV ++++   +    ++H+VYSLSKDLGLPGFRVG IYSN++ VV+AATKMSSFG
Sbjct: 241 FTSVLEVAKDKNMGLD---GKIHVVYSLSKDLGLPGFRVGLIYSNNEKVVSAATKMSSFG 300

Query: 303 LVSSQTQYLLSAMLSDKKFTRNYITENQRRLKQRQKMLVSGLEKAGINCLDSNAGLFCWV 362
           L+SSQTQ+LL+ +LSD++FT NY+ EN++RL++R+  LVSGL++AGI+CL SNAGLFCWV
Sbjct: 301 LISSQTQHLLANLLSDERFTTNYLEENKKRLRERKDRLVSGLKEAGISCLKSNAGLFCWV 360

Query: 363 DMRHLLESDTFESELELWKKIVYEVGLNISPGSSCHCSEPGWFRVCFANMSESTLKLAIR 422
           D+RHLL+S+TFE+E  LW KIV EVGLNISPGSSCHC EPGWFRVCFANMS+ T+++A+ 
Sbjct: 361 DLRHLLKSNTFEAEHSLWTKIVCEVGLNISPGSSCHCDEPGWFRVCFANMSDQTMEVAMD 420

Query: 423 RLKSFVAELRSAASPNAQSQLNNDNKFCKNI----KKNIFTKWVFRLS 467
           R+K FV               NN  K  + +    ++++  KWV +LS
Sbjct: 421 RVKGFVDN-------------NNGGKQKRTMWDTRRRSLINKWVSKLS 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q002578.2e-25590.791-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucurbita maxima OX=3661 GN... [more]
Q428811.4e-21475.481-aminocyclopropane-1-carboxylate synthase 3 OS=Solanum lycopersicum OX=4081 GN=... [more]
P378215.2e-20975.111-aminocyclopropane-1-carboxylate synthase OS=Malus domestica OX=3750 GN=ACS-1 P... [more]
Q9M2Y81.7e-20474.681-aminocyclopropane-1-carboxylate synthase 9 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q370013.9e-20473.391-aminocyclopropane-1-carboxylate synthase 5 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
XP_023544912.10.0100.001-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita pepo subsp. pe... [more]
XP_022950799.10.099.161-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita moschata][more]
KAG7034265.10.098.951-aminocyclopropane-1-carboxylate synthase, partial [Cucurbita argyrosperma subs... [more]
KAG6604101.10.098.951-aminocyclopropane-1-carboxylate synthase 101, partial [Cucurbita argyrosperma ... [more]
XP_022978599.10.098.331-aminocyclopropane-1-carboxylate synthase CMA101-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GGW60.099.161-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita moschata OX=... [more]
A0A6J1IUH10.098.331-aminocyclopropane-1-carboxylate synthase CMA101-like OS=Cucurbita maxima OX=36... [more]
I1VWI50.093.311-aminocyclopropane-1-carboxylate synthase OS=Citrullus lanatus subsp. vulgaris ... [more]
A0A5A7SZ750.092.101-aminocyclopropane-1-carboxylate synthase CMA101 OS=Cucumis melo var. makuwa OX... [more]
Q09PK30.091.481-aminocyclopropane-1-carboxylate synthase OS=Cucumis sativus OX=3659 GN=ACS1 PE... [more]
Match NameE-valueIdentityDescription
AT3G49700.11.2e-20574.681-aminocyclopropane-1-carboxylate synthase 9 [more]
AT5G65800.12.8e-20573.39ACC synthase 5 [more]
AT4G37770.11.5e-20372.711-amino-cyclopropane-1-carboxylate synthase 8 [more]
AT2G22810.11.0e-19170.061-aminocyclopropane-1-carboxylate synthase 4 [more]
AT4G08040.17.1e-17763.031-aminocyclopropane-1-carboxylate synthase 11 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00753ACCSYNTHASEcoord: 225..248
score: 67.35
coord: 263..287
score: 83.28
coord: 135..156
score: 86.36
coord: 43..69
score: 66.42
coord: 189..213
score: 68.66
coord: 295..318
score: 81.76
coord: 76..92
score: 74.54
coord: 113..133
score: 66.9
NoneNo IPR availablePANTHERPTHR43795BIFUNCTIONAL ASPARTATE AMINOTRANSFERASE AND GLUTAMATE/ASPARTATE-PREPHENATE AMINOTRANSFERASE-RELATEDcoord: 1..442
NoneNo IPR availablePANTHERPTHR43795:SF411-AMINOCYCLOPROPANE-1-CARBOXYLATE SYNTHASEcoord: 1..442
NoneNo IPR availableCDDcd00609AAT_likecoord: 45..425
e-value: 1.10481E-64
score: 211.046
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 63..318
e-value: 3.3E-169
score: 564.7
IPR004839Aminotransferase, class I/classIIPFAMPF00155Aminotran_1_2coord: 41..424
e-value: 1.6E-105
score: 353.3
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 29..423
e-value: 3.3E-169
score: 564.7
IPR004838Aminotransferases, class-I, pyridoxal-phosphate-binding sitePROSITEPS00105AA_TRANSFER_CLASS_1coord: 269..282
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 3..428

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g01970.1Cp4.1LG10g01970.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042218 1-aminocyclopropane-1-carboxylate biosynthetic process
biological_process GO:0009058 biosynthetic process
molecular_function GO:0016847 1-aminocyclopropane-1-carboxylate synthase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0008483 transaminase activity
molecular_function GO:0003824 catalytic activity