Cp4.1LG10g01740 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g01740
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionPhosphoenolpyruvate carboxykinase (ATP)
LocationCp4.1LG10: 3059096 .. 3062599 (-)
RNA-Seq ExpressionCp4.1LG10g01740
SyntenyCp4.1LG10g01740
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTTGTTTCCTAATAAATTGGTGGGTTGACCTTATTTGGGAAAAAGAAAAGAGAGATATGACAATGATCCGGAGACAAGGGGAATGAACATCACAAAATCTAACTCTAGGTTACCCGTGTACTTCTCACATATACTTGGAAATTTGGAGAAGACTTTTAACCATGGTCAAGTTAGACCCTGGTTAATTAACTGCTAAAGCTGTGCTCCTGAAGCCTTTAAAATGGGATCCCCCACAACTCCCATTTCGCACTCTCATCCGCTCTGTATCTCCACACTTTGGATGATCCAAGCTTTCACCTAAACTTCTGTGCCTGTACATTTCTCTTATTCATATTCCCTCTGTCTAAACAACTATTCCATTCCGATGGCCGATTCCAAGATGAAGAACGGGAACGGAGTTGCCAACGGATCTTCCACGCAAGTTCTGACGTCGCCGAAGCATGGAAATGGAATCTGCCACGACGGCAGCTCGTCGCCTATCAAGGCCCAAACCATCGATGAACTTCATAGCCTGCAGAAGAAGCGATCCACGCCCACTACTCCCACCGGCTCCTCTCCCTCGGCCTTCTCTGTTCTTTCAGAAGAAGATCGCCAGAGGCAGCAGCTCCAATCAATCAGGTACAACACATGCAACCAAAAAGAAACCACCGAAATACGGCTTCGTTTTGTTTTAAATATAAGATTTGTTTGGGTGGAAATTGTGATTCAGTGCATCGTTAGCATCGTTGACGAGAGAAACTGGGCCAAAAGTGGTGAAAGGAGACCCGGCTCGAAAAACTCCAACTTCGAAGATTGCACACGTGGCGCACCACCAATTCGCACCCACCATCAACATCAGCGACAGCTCATTGAAATTCACACACGTCCTATACAATCTCTCCCCTGCAGGTCCAAAGAAAACATAAAATTCCATTATTTAATCTAAATATATTTTATCATTTTTAGCTGAATGTATACGGCGTCGTTTTGCAGAGTTGTACGAGCAGGCCATAAGGCACGAGAGGGGCTCGTTTTTTACATCCACAGGAGCATTGGCCACGCTTTCTGGAGCCAAGACCGGACGGTCTCCAAGAGACAAGCGAATTGTTAAGGATGCGAATACCGAGAAAGAGCTCTGGTGGGGAAAGTAAGCATCATCCTTCTTCAAACCACGCCGTCTTCCACAGTTTGGTGTTTACCTCATTCGATCATTATTAATAATCTCTGCCTTTTTGTTTTGGACTGCAGGGGATCTCCAAACATTGAAATGGATGAGCACACTTTCACGGTCAACAGAGAAAGAGCCGTGGATTACTTGAACTCTCTCGACAAGGTCTTCGTCAACGACCAATTCTTGAATTGGGATCCCCAGAACAAACTCAAAGTCCGAATTGTCTCTGCCAGGGCTTACCACTCGTTGTTCATGCATAACATGTAAGTAACACAAAAATACTTGAGCCTTTTAAAACGGCGGTTAAATTAAATTGATGGGTTTTGTTTTGGTAGGTGCATCCGGCCAACGGAGGAAGAGTTGGAGAATTTTGGGACGCCGGATTTCACAATATACAATGCCGGACAGTTCCCCTGCAACCGTTTCACTCATTACATGACATCTTCAACCAGCATAGATATGAATCTGGCTAGGAGGGAAATGGTGATCCTTGGAACCCAATACGCGGGTGAGATGAAGAAGGGGCTGTTCAGTCTTATGCACTATCTCATGCCCAAACGACAGATTCTCTCCTTGCATTCTGGCTGCAACATGGGAAAAGATGGCGACGTCGCCCTCTTCTTTGGATTATCAGGTTCACACCAACATCTTTTTTTTTTGACAAATTTAATATTATGTTGTCTCTTAAAATTATTCCTTTTCTTTTAGGTACCGGAAAGACTACTTTATCCACCGATCACAACCGTTATTTGATTGGAGATGATGAGCATTGTTGGGGCGACAATGGTGTCTCAAATATCGAAGGAGGTTGCTATGCTAAGTGCATTGACCTTTCTCGAGAGAAAGAGCCTGATATTTGGAACGCCATCAAATTTGGAACTGGTAATAGCAATAACAACTCTCCTTCTCGGGAATTTTGTGTGAGAGTAAGTCTCTTTTATTGAAATGGTTCGTTTGGTTTGATAGTGTTAGAAAATGTCGTGTTTGATGAGCACACCAGAGAAGTGGACTATTCGGACAATTCAGTTACAGGTATTAATTAATTAAAATAGGAATCAATTATCATAATTGATATATATATATATATATATATATATATTTTTGTTTTTAATGACACAGAGAATACTCGAGCGGCGTACCCAATCGAGTACATACCGAACGCTAAGATACCATGCGTGGGGCCACACCCAAAGAACGTTATATTATTGGCTTGTGACGCATTTGGGGTGCTTCCACCAGTGAGCAAGCTCAATTTGGCTCAAACCATGTACCATTTCATCAGTGGATATACTGCATTGGTAAAAATAAAAAATAAACTTTTTAAATTGTAAGTAATTACTTTGTTGGACGAAGGCTGATTCGAAATTGAAATTGTAGGTGGCTGGAACCGAAGACGGTATAAAAGAGCCACAAGCGACATTCTCGGCTTGCTTTGGTGCGGCTTTCATTATGTTACATCCCACAAAGTACGCTGCCATGCTCGCCCAAAAGATGCAGAAGCACGGTGCCACCGGCTGGCTAGTCAACACCGGCTGGTCTGGCGGCCGATATGGCTCCGGTAGCCGTATTAAGCTTTCTTACACTAGAAAAATCATCGACGCCATTCATTCCGGCGAGCTTCTCAAAGCGAATTACCGTAAAACTAAAGTGTTTGGCCTCGAAATCCCCACCGCCATTGAAGGAGTTCCCTCCGAGATTTTGGACCCAATAAGCACGGTAAGAAAAGTGATATTATAATCTATACTATCTAGTAATTAAAAACTTTCTTCCATTATTATTATTATTATTTTTTTTTTTTGCAGTGGTCGAACAAAGATGCGTACAATGAAACGCTGCTGAAACTGGCTACTCTTTTCAAGAACAATTTCGAGACATTTACCAACTACAAGATTGGTGAAGACAATACTCTGGCTGAGCAAATCCTTGCTGCTGGTCCCATTTTTTAACCCAACAAAAAGGGGTTTACANATAAACATTTCTTATAAGGGTGTGAAAATCTATTTCTAATTTATGTATTTTAAAAATTTTGTGAAAAACTCGAAAGGAAAAATTCAAAGATGTGATAGGCTTGAATTATTACCAATTATATTCAGACACCGGCCGTGTGCCAATAAGAATGTTGGTTCCCAAAACACATTTCTTATAAAACTTTCCCTCAAAAATTTTAATTAGGTCAAATATATATTTTTAACATGAACATCTAATAATTAACCAATATAAATTTCAAAGAATAAAGAATAGAGGTAATTAATCTCCCTCTTAAATCAAATAATAGAGGTAATTAATTAACGTAATGGAAGAAGTACAGTTATTATTATTGTTTTTTAATTGTTTAAGATTTGATTCTCTAG

mRNA sequence

ATGGTTATGAAGAACGGGAACGGAGTTGCCAACGGATCTTCCACGCAAGTTCTGACGTCGCCGAAGCATGGAAATGGAATCTGCCACGACGGCAGCTCGTCGCCTATCAAGGCCCAAACCATCGATGAACTTCATAGCCTGCAGAAGAAGCGATCCACGCCCACTACTCCCACCGGCTCCTCTCCCTCGGCCTTCTCTGTTCTTTCAGAAGAAGATCGCCAGAGGCAGCAGCTCCAATCAATCAGTGCATCGTTAGCATCGTTGACGAGAGAAACTGGGCCAAAAGTGGTGAAAGGAGACCCGGCTCGAAAAACTCCAACTTCGAAGATTGCACACGTGGCGCACCACCAATTCGCACCCACCATCAACATCAGCGACAGCTCATTGAAATTCACACACGTCCTATACAATCTCTCCCCTGCAGAGTTGTACGAGCAGGCCATAAGGCACGAGAGGGGCTCGTTTTTTACATCCACAGGAGCATTGGCCACGCTTTCTGGAGCCAAGACCGGACGGTCTCCAAGAGACAAGCGAATTGTTAAGGATGCGAATACCGAGAAAGAGCTCTGGTGGGGAAAGGGATCTCCAAACATTGAAATGGATGAGCACACTTTCACGGTCAACAGAGAAAGAGCCGTGGATTACTTGAACTCTCTCGACAAGGTCTTCGTCAACGACCAATTCTTGAATTGGGATCCCCAGAACAAACTCAAAGTCCGAATTGTCTCTGCCAGGGCTTACCACTCGTTGTTCATGCATAACATGTGCATCCGGCCAACGGAGGAAGAGTTGGAGAATTTTGGGACGCCGGATTTCACAATATACAATGCCGGACAGTTCCCCTGCAACCGTTTCACTCATTACATGACATCTTCAACCAGCATAGATATGAATCTGGCTAGGAGGGAAATGGTGATCCTTGGAACCCAATACGCGGGTGAGATGAAGAAGGGGCTGTTCAGTCTTATGCACTATCTCATGCCCAAACGACAGATTCTCTCCTTGCATTCTGGCTGCAACATGGGAAAAGATGGCGACGTCGCCCTCTTCTTTGGATTATCAGGTACCGGAAAGACTACTTTATCCACCGATCACAACCGTTATTTGATTGGAGATGATGAGCATTGTTGGGGCGACAATGGTGTCTCAAATATCGAAGGAGGTTGCTATGCTAAGTGCATTGACCTTTCTCGAGAGAAAGAGCCTGATATTTGGAACGCCATCAAATTTGGAACTGTGTTAGAAAATGTCGTGTTTGATGAGCACACCAGAGAAGTGGACTATTCGGACAATTCAGTTACAGAGAATACTCGAGCGGCGTACCCAATCGAGTACATACCGAACGCTAAGATACCATGCGTGGGGCCACACCCAAAGAACGTTATATTATTGGCTTGTGACGCATTTGGGGTGCTTCCACCAGTGAGCAAGCTCAATTTGGCTCAAACCATGTACCATTTCATCAGTGGATATACTGCATTGGTGGCTGGAACCGAAGACGGTATAAAAGAGCCACAAGCGACATTCTCGGCTTGCTTTGGTGCGGCTTTCATTATGTTACATCCCACAAAGTACGCTGCCATGCTCGCCCAAAAGATGCAGAAGCACGGTGCCACCGGCTGGCTAGTCAACACCGGCTGGTCTGGCGGCCGATATGGCTCCGGTAGCCGTATTAAGCTTTCTTACACTAGAAAAATCATCGACGCCATTCATTCCGGCGAGCTTCTCAAAGCGAATTACCGTAAAACTAAAGTGTTTGGCCTCGAAATCCCCACCGCCATTGAAGGAGTTCCCTCCGAGATTTTGGACCCAATAAGCACGTGGTCGAACAAAGATGCGTACAATGAAACGCTGCTGAAACTGGCTACTCTTTTCAAGAACAATTTCGAGACATTTACCAACTACAAGATTGATTTGATTCTCTAG

Coding sequence (CDS)

ATGGTTATGAAGAACGGGAACGGAGTTGCCAACGGATCTTCCACGCAAGTTCTGACGTCGCCGAAGCATGGAAATGGAATCTGCCACGACGGCAGCTCGTCGCCTATCAAGGCCCAAACCATCGATGAACTTCATAGCCTGCAGAAGAAGCGATCCACGCCCACTACTCCCACCGGCTCCTCTCCCTCGGCCTTCTCTGTTCTTTCAGAAGAAGATCGCCAGAGGCAGCAGCTCCAATCAATCAGTGCATCGTTAGCATCGTTGACGAGAGAAACTGGGCCAAAAGTGGTGAAAGGAGACCCGGCTCGAAAAACTCCAACTTCGAAGATTGCACACGTGGCGCACCACCAATTCGCACCCACCATCAACATCAGCGACAGCTCATTGAAATTCACACACGTCCTATACAATCTCTCCCCTGCAGAGTTGTACGAGCAGGCCATAAGGCACGAGAGGGGCTCGTTTTTTACATCCACAGGAGCATTGGCCACGCTTTCTGGAGCCAAGACCGGACGGTCTCCAAGAGACAAGCGAATTGTTAAGGATGCGAATACCGAGAAAGAGCTCTGGTGGGGAAAGGGATCTCCAAACATTGAAATGGATGAGCACACTTTCACGGTCAACAGAGAAAGAGCCGTGGATTACTTGAACTCTCTCGACAAGGTCTTCGTCAACGACCAATTCTTGAATTGGGATCCCCAGAACAAACTCAAAGTCCGAATTGTCTCTGCCAGGGCTTACCACTCGTTGTTCATGCATAACATGTGCATCCGGCCAACGGAGGAAGAGTTGGAGAATTTTGGGACGCCGGATTTCACAATATACAATGCCGGACAGTTCCCCTGCAACCGTTTCACTCATTACATGACATCTTCAACCAGCATAGATATGAATCTGGCTAGGAGGGAAATGGTGATCCTTGGAACCCAATACGCGGGTGAGATGAAGAAGGGGCTGTTCAGTCTTATGCACTATCTCATGCCCAAACGACAGATTCTCTCCTTGCATTCTGGCTGCAACATGGGAAAAGATGGCGACGTCGCCCTCTTCTTTGGATTATCAGGTACCGGAAAGACTACTTTATCCACCGATCACAACCGTTATTTGATTGGAGATGATGAGCATTGTTGGGGCGACAATGGTGTCTCAAATATCGAAGGAGGTTGCTATGCTAAGTGCATTGACCTTTCTCGAGAGAAAGAGCCTGATATTTGGAACGCCATCAAATTTGGAACTGTGTTAGAAAATGTCGTGTTTGATGAGCACACCAGAGAAGTGGACTATTCGGACAATTCAGTTACAGAGAATACTCGAGCGGCGTACCCAATCGAGTACATACCGAACGCTAAGATACCATGCGTGGGGCCACACCCAAAGAACGTTATATTATTGGCTTGTGACGCATTTGGGGTGCTTCCACCAGTGAGCAAGCTCAATTTGGCTCAAACCATGTACCATTTCATCAGTGGATATACTGCATTGGTGGCTGGAACCGAAGACGGTATAAAAGAGCCACAAGCGACATTCTCGGCTTGCTTTGGTGCGGCTTTCATTATGTTACATCCCACAAAGTACGCTGCCATGCTCGCCCAAAAGATGCAGAAGCACGGTGCCACCGGCTGGCTAGTCAACACCGGCTGGTCTGGCGGCCGATATGGCTCCGGTAGCCGTATTAAGCTTTCTTACACTAGAAAAATCATCGACGCCATTCATTCCGGCGAGCTTCTCAAAGCGAATTACCGTAAAACTAAAGTGTTTGGCCTCGAAATCCCCACCGCCATTGAAGGAGTTCCCTCCGAGATTTTGGACCCAATAAGCACGTGGTCGAACAAAGATGCGTACAATGAAACGCTGCTGAAACTGGCTACTCTTTTCAAGAACAATTTCGAGACATTTACCAACTACAAGATTGATTTGATTCTCTAG

Protein sequence

MVMKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSPSAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTINISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAYNETLLKLATLFKNNFETFTNYKIDLIL
Homology
BLAST of Cp4.1LG10g01740 vs. ExPASy Swiss-Prot
Match: Q9T074 (Phosphoenolpyruvate carboxykinase (ATP) 1 OS=Arabidopsis thaliana OX=3702 GN=PCK1 PE=1 SV=1)

HSP 1 Score: 1068.5 bits (2762), Expect = 2.8e-311
Identity = 517/623 (82.99%), Postives = 569/623 (91.33%), Query Frame = 0

Query: 22  KHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTG-SSPSAFSVLSEEDRQRQQLQS 81
           K G+G+CHD S   + A TIDELHSLQKKRS PTTP   ++ +AF+ +SEE+RQ+ QLQS
Sbjct: 32  KRGSGVCHDDSGPTVNATTIDELHSLQKKRSAPTTPINQNAAAAFAAVSEEERQKIQLQS 91

Query: 82  ISASLASLTRETGPKVVKGDPARK----TPTSKIAHVAHHQ-FAP-TINISDSSLKFTHV 141
           ISASLASLTRE+GPKVV+GDPA K    + T   AH  HH  F+P T  +SDSSLKFTHV
Sbjct: 92  ISASLASLTRESGPKVVRGDPAEKKTDGSTTPAYAHGQHHSIFSPATGAVSDSSLKFTHV 151

Query: 142 LYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKG 201
           LYNLSPAELYEQAI++E+GSF TS GALATLSGAKTGR+PRDKR+V+DA TE ELWWGKG
Sbjct: 152 LYNLSPAELYEQAIKYEKGSFITSNGALATLSGAKTGRAPRDKRVVRDATTEDELWWGKG 211

Query: 202 SPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHN 261
           SPNIEMDEHTF VNRERAVDYLNSL+KVFVNDQ+LNWDP+N++KVRIVSARAYHSLFMHN
Sbjct: 212 SPNIEMDEHTFMVNRERAVDYLNSLEKVFVNDQYLNWDPENRIKVRIVSARAYHSLFMHN 271

Query: 262 MCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGE 321
           MCIRPT+EELE+FGTPDFTIYNAGQFPCNR+THYMTSSTS+D+NLARREMVILGTQYAGE
Sbjct: 272 MCIRPTQEELESFGTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVILGTQYAGE 331

Query: 322 MKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDE 381
           MKKGLFS+MHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDE
Sbjct: 332 MKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDE 391

Query: 382 HCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVT 441
           HCW + GVSNIEGGCYAKC+DLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSD SVT
Sbjct: 392 HCWTETGVSNIEGGCYAKCVDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVT 451

Query: 442 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL 501
           ENTRAAYPIE+IPNAKIPCVGPHP NVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL
Sbjct: 452 ENTRAAYPIEFIPNAKIPCVGPHPTNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL 511

Query: 502 VAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGS 561
           VAGTEDGIKEP ATFSACFGAAFIMLHPTKYAAMLA+KM+  GATGWLVNTGWSGG YG 
Sbjct: 512 VAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMKSQGATGWLVNTGWSGGSYGV 571

Query: 562 GSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAY 621
           G+RIKL+YTRKIIDAIHSG LLKANY+KT++FG EIPT IEG+PSEILDP+++WS+K A+
Sbjct: 572 GNRIKLAYTRKIIDAIHSGSLLKANYKKTEIFGFEIPTEIEGIPSEILDPVNSWSDKKAH 631

Query: 622 NETLLKLATLFKNNFETFTNYKI 638
            +TL+KL  LFK NFE F N+KI
Sbjct: 632 KDTLVKLGGLFKKNFEVFANHKI 654

BLAST of Cp4.1LG10g01740 vs. ExPASy Swiss-Prot
Match: P42066 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=PCK PE=2 SV=1)

HSP 1 Score: 1064.3 bits (2751), Expect = 5.4e-310
Identity = 513/620 (82.74%), Postives = 564/620 (90.97%), Query Frame = 0

Query: 20  SPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSPSAFSVLSEEDRQRQQLQ 79
           +P     ICHD S++P++A+T++ LHSLQKKRSTPTTP   S   FS +SE +RQ+QQL 
Sbjct: 35  APTTERDICHDDSTTPMRARTLEHLHSLQKKRSTPTTPLTDSQGVFSPVSEAERQKQQLI 94

Query: 80  SISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFA-PTINISDSSLKFTHVLYNL 139
           SISASLASLTRETGPK+VKGDP +K    K + + H  F  P +N+SDS+LK TH+LYN 
Sbjct: 95  SISASLASLTRETGPKLVKGDPEKKKEAHKASVLDHLHFGEPILNLSDSALKSTHILYNP 154

Query: 140 SPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNI 199
           SPAELYEQAI++E+GSF TSTGALATLSGAKTGRSP DKR+VKD  TEKELWWGKGSPNI
Sbjct: 155 SPAELYEQAIKYEKGSFITSTGALATLSGAKTGRSPIDKRVVKDDTTEKELWWGKGSPNI 214

Query: 200 EMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIR 259
           EMDEHTF +NRERAVDYLNSLDKVFVNDQFLNWDP+N++KVRIVSARAYHSLFMHNMCIR
Sbjct: 215 EMDEHTFLINRERAVDYLNSLDKVFVNDQFLNWDPENRIKVRIVSARAYHSLFMHNMCIR 274

Query: 260 PTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGEMKKG 319
           PT  ELE+FGTPDFTIY+AGQFPCNR+THYMTSSTSIDMNL R+EMVILGTQYAGEMKKG
Sbjct: 275 PTAGELEDFGTPDFTIYHAGQFPCNRYTHYMTSSTSIDMNLDRKEMVILGTQYAGEMKKG 334

Query: 320 LFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWG 379
           LFSLMHYLMP RQILSLHSGCNMGK+GDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW 
Sbjct: 335 LFSLMHYLMPMRQILSLHSGCNMGKNGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWS 394

Query: 380 DNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVTENTR 439
           DNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYS+ SVTENTR
Sbjct: 395 DNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSEKSVTENTR 454

Query: 440 AAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGT 499
           AAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKL+L QTMYHFISGYTALVAGT
Sbjct: 455 AAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLSLPQTMYHFISGYTALVAGT 514

Query: 500 EDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRI 559
           E+G+KEPQATFSACFGAAFIMLHP++YAAMLA+KM+KHGATGWLVNTGWSGG YGSG+RI
Sbjct: 515 EEGVKEPQATFSACFGAAFIMLHPSRYAAMLAEKMKKHGATGWLVNTGWSGGSYGSGNRI 574

Query: 560 KLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAYNETL 619
           KL+YTRKIIDAIHSG LL+ANY KT+VFGLEIP AIEGVPS ILDPI+TWS+KD Y+ETL
Sbjct: 575 KLAYTRKIIDAIHSGALLEANYSKTRVFGLEIPDAIEGVPSHILDPINTWSDKDGYHETL 634

Query: 620 LKLATLFKNNFETFTNYKID 639
           LKL  LFK N+E    Y+++
Sbjct: 635 LKLGGLFKKNYEGIHTYQVE 654

BLAST of Cp4.1LG10g01740 vs. ExPASy Swiss-Prot
Match: Q9SLZ0 (Phosphoenolpyruvate carboxykinase (ATP) OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 6.4e-303
Identity = 523/653 (80.09%), Postives = 568/653 (86.98%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           M   NG+A   +T      K  NG+ +D SS+P++AQTIDELHSLQ+KRS PTTP  S+P
Sbjct: 1   MATPNGLARIETT---GKKKQDNGVWYDDSSAPVRAQTIDELHSLQRKRSAPTTPNRSAP 60

Query: 63  ---------SAFSV-LSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARK-------T 122
                    S F+V +SEE+R  QQ+QSISASLASLTRETGPKVVKGDPA K        
Sbjct: 61  TTPIKGGAHSPFAVAISEEERHTQQMQSISASLASLTRETGPKVVKGDPAAKGEAAAQGA 120

Query: 123 PTSKIAHVAH-HQFAPTINISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALAT 182
           P++  AH  H H  AP I +SDSSLKFTHVL NLSPAELYEQAI++E+GSF TSTGALAT
Sbjct: 121 PSTPRAHQQHRHPAAPAIAVSDSSLKFTHVLNNLSPAELYEQAIKYEKGSFITSTGALAT 180

Query: 183 LSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFV 242
           LSGAKTGRSPRDKR+VKD  T ++LWWGKGSPNIEMDE TF +NRERAVDYLNSL KV  
Sbjct: 181 LSGAKTGRSPRDKRVVKDEVTAQDLWWGKGSPNIEMDEKTFLINRERAVDYLNSL-KVVR 240

Query: 243 NDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNR 302
             Q LNWDP+N++KVRI+SARAYHSLFMHNMCIRPT+EELE+FGTPDFTIYNAGQFPCNR
Sbjct: 241 QRQLLNWDPENRIKVRIISARAYHSLFMHNMCIRPTDEELEDFGTPDFTIYNAGQFPCNR 300

Query: 303 FTHYMTSSTSIDMNLARREMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKD 362
           +THYMTSSTSID+NLARREMVI+GTQYAGEMKKGLF +MHYLMPKR ILSLHSGCNMGKD
Sbjct: 301 YTHYMTSSTSIDLNLARREMVIMGTQYAGEMKKGLFGVMHYLMPKRGILSLHSGCNMGKD 360

Query: 363 GDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDI 422
           GDVALFFGLSGTGKTTLSTDHN  LIGDDEHCW DNGVSNIEGGCYAKCIDL++EKEPDI
Sbjct: 361 GDVALFFGLSGTGKTTLSTDHNGLLIGDDEHCWSDNGVSNIEGGCYAKCIDLAQEKEPDI 420

Query: 423 WNAIKFGTVLENVVFDEHTREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILL 482
           WNAIKFGTVLENVVFDEHTREVDY+D SVTENTRAAYPIEYIP AKIPCVGPHPKNVILL
Sbjct: 421 WNAIKFGTVLENVVFDEHTREVDYADYSVTENTRAAYPIEYIPIAKIPCVGPHPKNVILL 480

Query: 483 ACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTK 542
           ACDAFGVLPPVSKL LAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTK
Sbjct: 481 ACDAFGVLPPVSKLILAQTMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTK 540

Query: 543 YAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTK 602
           YAAMLA+KMQK+GATGWLVNTGWSGGRYG G RI+L YTRKIIDAIHSGELL ANY+KT+
Sbjct: 541 YAAMLAEKMQKYGATGWLVNTGWSGGRYGVGKRIRLPYTRKIIDAIHSGELLTANYQKTE 600

Query: 603 VFGLEIPTAIEGVPSEILDPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 638
           VFGLEIPT I GVPSEILDPI TW++K AY E LL+L  LFKNNFE F +YKI
Sbjct: 601 VFGLEIPTEINGVPSEILDPIYTWTDKAAYKENLLRLGGLFKNNFEVFASYKI 649

BLAST of Cp4.1LG10g01740 vs. ExPASy Swiss-Prot
Match: B5X574 (Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Arabidopsis thaliana OX=3702 GN=PCK2 PE=1 SV=1)

HSP 1 Score: 1026.9 bits (2654), Expect = 9.6e-299
Identity = 499/616 (81.01%), Postives = 548/616 (88.96%), Query Frame = 0

Query: 27  ICHDGSSSPIKAQTIDELHSLQKKRSTPTTP----TGSSPSAFSVLSEEDRQRQQLQSIS 86
           +C D  +  +  QTIDELHSLQKKRS PTTP    + S  S  S  +        LQS+S
Sbjct: 41  VCQDDIAPRVNFQTIDELHSLQKKRSAPTTPLRDGSASGVSGTSGPTTPVSSETMLQSVS 100

Query: 87  ASLASLTRETGPKVVKGDPARKTPTSKIAHV-AHHQFAPTINISDSSLKFTHVLYNLSPA 146
           ASLASLTRETGPK+++GDP   T  +K+AHV       P  ++SDS LKFTH+L+NLSPA
Sbjct: 101 ASLASLTRETGPKLIRGDP---TSAAKVAHVPVTPTSLPAADVSDSGLKFTHILHNLSPA 160

Query: 147 ELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNIEMD 206
           ELYEQAI+ E+GSF TSTGALATLSGAKTGRSP+DKR+VKD  TE ELWWGKGSPNIEMD
Sbjct: 161 ELYEQAIKFEKGSFVTSTGALATLSGAKTGRSPKDKRVVKDDTTEAELWWGKGSPNIEMD 220

Query: 207 EHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIRPTE 266
           E TF VNRERAVDYLNSLDKVFVNDQ+LNWDP+NK+KVRIVSARAYHSLFMHNMCIRPT 
Sbjct: 221 EKTFLVNRERAVDYLNSLDKVFVNDQYLNWDPENKIKVRIVSARAYHSLFMHNMCIRPTP 280

Query: 267 EELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGEMKKGLFS 326
           EELENFGTPDFTIYNAG+FPCNRFTHYMTSSTS+D+NL RREMVILGTQYAGEMKKGLF 
Sbjct: 281 EELENFGTPDFTIYNAGKFPCNRFTHYMTSSTSVDINLGRREMVILGTQYAGEMKKGLFG 340

Query: 327 LMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGDNG 386
           +MHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW + G
Sbjct: 341 VMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSEAG 400

Query: 387 VSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVTENTRAAY 446
           VSNIEGGCYAKCIDL+R+KEPDIWNAIKFGTVLENVVFDEHTREVDY+D SVTENTRAAY
Sbjct: 401 VSNIEGGCYAKCIDLARDKEPDIWNAIKFGTVLENVVFDEHTREVDYTDKSVTENTRAAY 460

Query: 447 PIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDG 506
           PIEYIPN+KIPCVGPHPKNVILLACDAFGVLPP+SKLNLAQTMYHFISGYTALVAGTE+G
Sbjct: 461 PIEYIPNSKIPCVGPHPKNVILLACDAFGVLPPISKLNLAQTMYHFISGYTALVAGTEEG 520

Query: 507 IKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRIKLS 566
           +KEP+ATFSACFGAAFIMLHPTKYAAMLA+KMQ  GATGWLVNTGWSGG YG+GSRIKL+
Sbjct: 521 VKEPRATFSACFGAAFIMLHPTKYAAMLAEKMQAQGATGWLVNTGWSGGSYGTGSRIKLA 580

Query: 567 YTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAYNETLLKL 626
           YTRKIIDAIHSG LL A+YRKT +FGLEIP  +EGVPSEIL+PI+ W +K AY +TLLKL
Sbjct: 581 YTRKIIDAIHSGSLLNASYRKTDIFGLEIPNEVEGVPSEILEPINAWPDKMAYEDTLLKL 640

Query: 627 ATLFKNNFETFTNYKI 638
           A LFK+NFETFT++KI
Sbjct: 641 AGLFKSNFETFTSHKI 653

BLAST of Cp4.1LG10g01740 vs. ExPASy Swiss-Prot
Match: Q9XFA2 (Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Urochloa panicoides OX=37563 GN=PCK2 PE=2 SV=1)

HSP 1 Score: 990.7 bits (2560), Expect = 7.6e-288
Identity = 486/612 (79.41%), Postives = 537/612 (87.75%), Query Frame = 0

Query: 29  HDG-SSSPIKAQTIDELHSLQKKRSTPTTPTGSSPSAFSVLSEEDRQRQQLQSISASLAS 88
           HD  S++P+ AQTI+ELHSLQ+K +T TT  G+SP               LQSISASLAS
Sbjct: 14  HDSDSAAPVNAQTIEELHSLQRKAAT-TTKDGASP---------------LQSISASLAS 73

Query: 89  LTRETGPKVVKGDP--ARKTPTSKIAHVAHHQFAPTINISDSSLKFTHVLYNLSPAELYE 148
           L RE GP +VKGDP   +  P   I H      A TI  SDSSLKFTHVLYNLSPAELYE
Sbjct: 74  LAREYGPNLVKGDPEATKGAPPVPIKHQQPSAAAATIAASDSSLKFTHVLYNLSPAELYE 133

Query: 149 QAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNIEMDEHTF 208
           QA   ++ SF TSTGALATLSGAKTGRSPRDKR+VKD  T +ELWWGKGSPNIEMDE  F
Sbjct: 134 QAFGQKKSSFITSTGALATLSGAKTGRSPRDKRVVKDETTSQELWWGKGSPNIEMDERQF 193

Query: 209 TVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIRPTEEELE 268
            +NRERA+DYLNSLDKV+VNDQFLNWD +N++KVRI+++RAYH+LFMHNMCIRPTEEELE
Sbjct: 194 VINRERALDYLNSLDKVYVNDQFLNWDSENRIKVRIITSRAYHALFMHNMCIRPTEEELE 253

Query: 269 NFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGEMKKGLFSLMHY 328
           +FGTPDFTIYNAG+FP NR+ +YMTSSTSI+++LARREMVILGTQYAGEMKKGLF +MHY
Sbjct: 254 SFGTPDFTIYNAGEFPANRYANYMTSSTSINISLARREMVILGTQYAGEMKKGLFGVMHY 313

Query: 329 LMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGDNGVSNI 388
           LMPKR ILSLHSGCNMGK+GDVALFFGLSGTGKTTLSTDHNR LIGDDEHCW DNGVSNI
Sbjct: 314 LMPKRGILSLHSGCNMGKEGDVALFFGLSGTGKTTLSTDHNRLLIGDDEHCWSDNGVSNI 373

Query: 389 EGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVTENTRAAYPIEY 448
           EGGCYAKCIDLS+EKEPDIWNAIKFGTVLENVVF+E TREVDY+D S+TENTRAAYPIE+
Sbjct: 374 EGGCYAKCIDLSQEKEPDIWNAIKFGTVLENVVFNERTREVDYADKSITENTRAAYPIEF 433

Query: 449 IPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIKEP 508
           IPNAKIPCVGPHPKNVILLACDA+GVLPPVSKLNLAQTMYHFISGYTA+VAGTEDG+KEP
Sbjct: 434 IPNAKIPCVGPHPKNVILLACDAYGVLPPVSKLNLAQTMYHFISGYTAIVAGTEDGVKEP 493

Query: 509 QATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRIKLSYTRK 568
            ATFSACFGAAFIM HPTKYAAMLA+KMQK+GATGWLVNTGWSGGRYG G+RIKL YTRK
Sbjct: 494 TATFSACFGAAFIMYHPTKYAAMLAEKMQKYGATGWLVNTGWSGGRYGVGNRIKLPYTRK 553

Query: 569 IIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAYNETLLKLATLF 628
           IIDAIHSGELL A+Y+KT+VFGLEIPTAI GVPSEIL P++TW++K AY ETLLKLA LF
Sbjct: 554 IIDAIHSGELLNASYKKTEVFGLEIPTAINGVPSEILGPVNTWTDKAAYKETLLKLAGLF 609

Query: 629 KNNFETFTNYKI 638
           KNNFE F +YKI
Sbjct: 614 KNNFEVFASYKI 609

BLAST of Cp4.1LG10g01740 vs. NCBI nr
Match: XP_023544791.1 (phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1293 bits (3346), Expect = 0.0
Identity = 635/635 (100.00%), Postives = 635/635 (100.00%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI
Sbjct: 66  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. NCBI nr
Match: XP_022950481.1 (phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita moschata])

HSP 1 Score: 1286 bits (3327), Expect = 0.0
Identity = 631/635 (99.37%), Postives = 633/635 (99.69%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           MKNGNGVANGSSTQV TSPKHGNG+CHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   MKNGNGVANGSSTQVQTSPKHGNGVCHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI
Sbjct: 66  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIR+ERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRYERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELE FGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELETFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. NCBI nr
Match: KAG6604082.1 (Phosphoenolpyruvate carboxykinase (ATP), partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1284 bits (3323), Expect = 0.0
Identity = 631/635 (99.37%), Postives = 631/635 (99.37%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           MKNGNGVANGSSTQV TSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   MKNGNGVANGSSTQVQTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPT KIAHVAHHQFAPTI
Sbjct: 66  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTPKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELE FGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELETFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMP RQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPMRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. NCBI nr
Match: KAG7034246.1 (Phosphoenolpyruvate carboxykinase (ATP), partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1283 bits (3319), Expect = 0.0
Identity = 630/635 (99.21%), Postives = 631/635 (99.37%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           MKNGNGVANGSSTQV TSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   MKNGNGVANGSSTQVQTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFSVLSEE+RQRQQLQSISASLASLTRETGPKVVKGDPARKTPT KIAHVAHHQFAPTI
Sbjct: 66  SAFSVLSEEERQRQQLQSISASLASLTRETGPKVVKGDPARKTPTPKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELE FGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELETFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMP RQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPMRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. NCBI nr
Match: XP_022977875.1 (phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita maxima])

HSP 1 Score: 1279 bits (3309), Expect = 0.0
Identity = 628/635 (98.90%), Postives = 631/635 (99.37%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           +KNGNGVANGSSTQV TSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   IKNGNGVANGSSTQVQTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFS LSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI
Sbjct: 66  SAFSALSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIR ERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRCERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFT+NRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTINRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE 
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEQ 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSN+DAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNRDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. ExPASy TrEMBL
Match: A0A6J1GEY4 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita moschata OX=3662 GN=LOC111453577 PE=3 SV=1)

HSP 1 Score: 1286 bits (3327), Expect = 0.0
Identity = 631/635 (99.37%), Postives = 633/635 (99.69%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           MKNGNGVANGSSTQV TSPKHGNG+CHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   MKNGNGVANGSSTQVQTSPKHGNGVCHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI
Sbjct: 66  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIR+ERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRYERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELE FGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELETFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. ExPASy TrEMBL
Match: A0A6J1INJ3 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita maxima OX=3661 GN=LOC111478035 PE=3 SV=1)

HSP 1 Score: 1279 bits (3309), Expect = 0.0
Identity = 628/635 (98.90%), Postives = 631/635 (99.37%), Query Frame = 0

Query: 3   MKNGNGVANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 62
           +KNGNGVANGSSTQV TSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP
Sbjct: 6   IKNGNGVANGSSTQVQTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSP 65

Query: 63  SAFSVLSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 122
           SAFS LSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI
Sbjct: 66  SAFSALSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTI 125

Query: 123 NISDSSLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 182
           NISDSSLKFTHVLYNLSPAELYEQAIR ERGSFFTSTGALATLSGAKTGRSPRDKRIVKD
Sbjct: 126 NISDSSLKFTHVLYNLSPAELYEQAIRCERGSFFTSTGALATLSGAKTGRSPRDKRIVKD 185

Query: 183 ANTEKELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 242
           ANTEKELWWGKGSPNIEMDEHTFT+NRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV
Sbjct: 186 ANTEKELWWGKGSPNIEMDEHTFTINRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIV 245

Query: 243 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 302
           SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR
Sbjct: 246 SARAYHSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARR 305

Query: 303 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 362
           EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS
Sbjct: 306 EMVILGTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLS 365

Query: 363 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEH 422
           TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDE 
Sbjct: 366 TDHNRYLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEQ 425

Query: 423 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 482
           TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ
Sbjct: 426 TREVDYSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQ 485

Query: 483 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 542
           TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL
Sbjct: 486 TMYHFISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWL 545

Query: 543 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 602
           VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL
Sbjct: 546 VNTGWSGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEIL 605

Query: 603 DPISTWSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           DPISTWSN+DAYNETLLKLATLFKNNFETFTNYKI
Sbjct: 606 DPISTWSNRDAYNETLLKLATLFKNNFETFTNYKI 640

BLAST of Cp4.1LG10g01740 vs. ExPASy TrEMBL
Match: A0A1S3B0T3 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis melo OX=3656 GN=LOC103484904 PE=3 SV=1)

HSP 1 Score: 1182 bits (3058), Expect = 0.0
Identity = 572/630 (90.79%), Postives = 605/630 (96.03%), Query Frame = 0

Query: 9   VANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSPS-AFSV 68
           ++NG + ++ TS K GNG+CHD SSSPIKAQTIDELHSLQKKRSTPTTP GS+PS AFS+
Sbjct: 6   ISNGFAKKINTSTKQGNGVCHDDSSSPIKAQTIDELHSLQKKRSTPTTPIGSAPSSAFSL 65

Query: 69  LSEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTINISDS 128
           ++EEDRQRQQLQSISA+LASLTRETGPKVVKGDPARK  ++K+AHVAHHQ+AP INISDS
Sbjct: 66  ITEEDRQRQQLQSISATLASLTRETGPKVVKGDPARKETSTKVAHVAHHQYAPAINISDS 125

Query: 129 SLKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEK 188
           SLKFTHVLYNLSPAELYEQAIR+E+GSF TS GALATLSGAKTGRSP+DKR+VKD NTE+
Sbjct: 126 SLKFTHVLYNLSPAELYEQAIRYEKGSFLTSVGALATLSGAKTGRSPKDKRVVKDENTER 185

Query: 189 ELWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAY 248
           ELWWGKGSPNIEMDEHTF +NRERAVDYLNSLDKVFVNDQFLNWDP+NK+KVRIVSARAY
Sbjct: 186 ELWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIKVRIVSARAY 245

Query: 249 HSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVIL 308
           HSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSID+NLAR+EMVIL
Sbjct: 246 HSLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDVNLARKEMVIL 305

Query: 309 GTQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR 368
           GTQYAGEMKKGLFSLMHYLMPKR ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTD NR
Sbjct: 306 GTQYAGEMKKGLFSLMHYLMPKRHILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDQNR 365

Query: 369 YLIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD 428
           YLIGDDEHCW +NGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD
Sbjct: 366 YLIGDDEHCWSNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD 425

Query: 429 YSDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHF 488
           YSDNSVTENTRAAYPIEYIPNA IPCVGPHPKNVILLACDAFGVLPPVSKL+LAQTMYHF
Sbjct: 426 YSDNSVTENTRAAYPIEYIPNALIPCVGPHPKNVILLACDAFGVLPPVSKLSLAQTMYHF 485

Query: 489 ISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGW 548
           ISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KMQ HGATGWLVNTGW
Sbjct: 486 ISGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMQNHGATGWLVNTGW 545

Query: 549 SGGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPIST 608
           SGGRYG GSRIKLSYTRKIIDAIHSGELL ANY KTKVFGLEIPTAIEG+PSEILDP++T
Sbjct: 546 SGGRYGYGSRIKLSYTRKIIDAIHSGELLNANYCKTKVFGLEIPTAIEGIPSEILDPMNT 605

Query: 609 WSNKDAYNETLLKLATLFKNNFETFTNYKI 637
           W +KDAY+ETLLKLA LFKNNFETFTNYKI
Sbjct: 606 WPDKDAYDETLLKLANLFKNNFETFTNYKI 635

BLAST of Cp4.1LG10g01740 vs. ExPASy TrEMBL
Match: A0A0A0KJU6 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=Csa_6G497180 PE=3 SV=1)

HSP 1 Score: 1169 bits (3025), Expect = 0.0
Identity = 561/629 (89.19%), Postives = 602/629 (95.71%), Query Frame = 0

Query: 9   VANGSSTQVLTSPKHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSPSAFSVL 68
           ++NG S ++ TS K GNG+C DGSSSPIKAQTIDELHSLQKKRSTP+TP+GS  SAFSV+
Sbjct: 6   ISNGFSKKINTSMKQGNGVCQDGSSSPIKAQTIDELHSLQKKRSTPSTPSGSVSSAFSVI 65

Query: 69  SEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTINISDSS 128
           +EEDRQRQQLQSISASLASLTRETGPKVVKGDPARK  ++K+AHV HHQ  P I+ISDSS
Sbjct: 66  TEEDRQRQQLQSISASLASLTRETGPKVVKGDPARKETSTKVAHVVHHQLGPNISISDSS 125

Query: 129 LKFTHVLYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKE 188
           +KFTHVLYNLSP ELYEQAIR+E+GSF TSTGALATLSGAKTGRSPRDKR+VKD NTEKE
Sbjct: 126 MKFTHVLYNLSPGELYEQAIRYEKGSFLTSTGALATLSGAKTGRSPRDKRVVKDENTEKE 185

Query: 189 LWWGKGSPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYH 248
           LWWGKGSPNIEMDEHTF +NRERAVDYLNSLDKVFVNDQFLNWDP+NK++VRIVSARAYH
Sbjct: 186 LWWGKGSPNIEMDEHTFMINRERAVDYLNSLDKVFVNDQFLNWDPENKIRVRIVSARAYH 245

Query: 249 SLFMHNMCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILG 308
           SLFMHNMCIRPTEEELENFGTPDFTIYN+GQFPCNRFTHYMTSSTSID++LAR+EMVILG
Sbjct: 246 SLFMHNMCIRPTEEELENFGTPDFTIYNSGQFPCNRFTHYMTSSTSIDVSLARKEMVILG 305

Query: 309 TQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRY 368
           TQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNR+
Sbjct: 306 TQYAGEMKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRF 365

Query: 369 LIGDDEHCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDY 428
           LIGDDEHCWG+NGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVD+
Sbjct: 366 LIGDDEHCWGNNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDF 425

Query: 429 SDNSVTENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFI 488
           +D SVTENTRAAYPIEYIPNA IPCVGPHPKN+ILLACDAFGVLPPVSKL LAQTMYHFI
Sbjct: 426 TDKSVTENTRAAYPIEYIPNALIPCVGPHPKNIILLACDAFGVLPPVSKLTLAQTMYHFI 485

Query: 489 SGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWS 548
           SGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLA+KM+ HGATGWLVNTGWS
Sbjct: 486 SGYTALVAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAEKMKTHGATGWLVNTGWS 545

Query: 549 GGRYGSGSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTW 608
           GGRYG GSRIKLSYTRKIIDAIHSGELL A+Y KTK+FGLEIP AIEG+PSEILDP +TW
Sbjct: 546 GGRYGYGSRIKLSYTRKIIDAIHSGELLNADYHKTKIFGLEIPNAIEGIPSEILDPTNTW 605

Query: 609 SNKDAYNETLLKLATLFKNNFETFTNYKI 637
           S+KDAY+ETLLKLA+LFKNNFETFT+Y+I
Sbjct: 606 SDKDAYDETLLKLASLFKNNFETFTSYRI 634

BLAST of Cp4.1LG10g01740 vs. ExPASy TrEMBL
Match: A0A6J1HFF7 (Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita moschata OX=3662 GN=LOC111463084 PE=3 SV=1)

HSP 1 Score: 1156 bits (2991), Expect = 0.0
Identity = 559/614 (91.04%), Postives = 585/614 (95.28%), Query Frame = 0

Query: 24  GNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTGSSPSAFSVLSEEDRQRQQLQSISA 83
           GN +CHD SSSPIKAQTIDELH LQKKRS+P+TP  SSP   S LSEEDRQRQQLQSISA
Sbjct: 11  GNEVCHDDSSSPIKAQTIDELHCLQKKRSSPSTPIDSSPGGVSALSEEDRQRQQLQSISA 70

Query: 84  SLASLTRETGPKVVKGDPARKTPTSKIAHVAHHQFAPTINISDSSLKFTHVLYNLSPAEL 143
           SLASLTRETGP VVKGDPARKT +SK+AH+A HQ  PTINISDSS+KFTHVLYNLSPAEL
Sbjct: 71  SLASLTRETGPNVVKGDPARKTGSSKVAHMAQHQLVPTINISDSSMKFTHVLYNLSPAEL 130

Query: 144 YEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNIEMDEH 203
           YEQAIRHE+GSF TSTGALATLSGAKTGRSPRDKR+VKD NT KELWWGKGSPNIEMDEH
Sbjct: 131 YEQAIRHEKGSFMTSTGALATLSGAKTGRSPRDKRVVKDENTTKELWWGKGSPNIEMDEH 190

Query: 204 TFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIRPTEEE 263
           TF +NRERAVDYLNSLDKVFVNDQFLNWDP+N++KVRIVSARAYHSLFMHNMCIRPT+ E
Sbjct: 191 TFMINRERAVDYLNSLDKVFVNDQFLNWDPENRIKVRIVSARAYHSLFMHNMCIRPTDAE 250

Query: 264 LENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGEMKKGLFSLM 323
           L NFG PDFTIYNAGQFPCNRFTHYMTSSTSIDMNLAR+EMVILGTQYAGEMKKGLFSLM
Sbjct: 251 LANFGRPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARKEMVILGTQYAGEMKKGLFSLM 310

Query: 324 HYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGDNGVS 383
           HYLMPKR+ILSLHSGCNMGK+GDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW + GVS
Sbjct: 311 HYLMPKREILSLHSGCNMGKNGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWTNTGVS 370

Query: 384 NIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVTENTRAAYPI 443
           NIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVF+EHTREVDYSDNSVTENTRAAYPI
Sbjct: 371 NIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFEEHTREVDYSDNSVTENTRAAYPI 430

Query: 444 EYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIK 503
           EYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIK
Sbjct: 431 EYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDGIK 490

Query: 504 EPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRIKLSYT 563
           EPQATFSACFGAAFIM+HPTKYAAMLA KM+KHGATGWLVNTGWSGGRYGSGSRIKLSYT
Sbjct: 491 EPQATFSACFGAAFIMMHPTKYAAMLADKMRKHGATGWLVNTGWSGGRYGSGSRIKLSYT 550

Query: 564 RKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAYNETLLKLAT 623
           RKIIDAIHSGELLKA +RKT+VFGLEIP AIEGVPSEILDP++TWS+K AY ETL KLAT
Sbjct: 551 RKIIDAIHSGELLKAQFRKTEVFGLEIPAAIEGVPSEILDPMNTWSDKGAYKETLQKLAT 610

Query: 624 LFKNNFETFTNYKI 637
           LFKNNFETFTNYKI
Sbjct: 611 LFKNNFETFTNYKI 624

BLAST of Cp4.1LG10g01740 vs. TAIR 10
Match: AT4G37870.1 (phosphoenolpyruvate carboxykinase 1 )

HSP 1 Score: 1068.5 bits (2762), Expect = 2.0e-312
Identity = 517/623 (82.99%), Postives = 569/623 (91.33%), Query Frame = 0

Query: 22  KHGNGICHDGSSSPIKAQTIDELHSLQKKRSTPTTPTG-SSPSAFSVLSEEDRQRQQLQS 81
           K G+G+CHD S   + A TIDELHSLQKKRS PTTP   ++ +AF+ +SEE+RQ+ QLQS
Sbjct: 32  KRGSGVCHDDSGPTVNATTIDELHSLQKKRSAPTTPINQNAAAAFAAVSEEERQKIQLQS 91

Query: 82  ISASLASLTRETGPKVVKGDPARK----TPTSKIAHVAHHQ-FAP-TINISDSSLKFTHV 141
           ISASLASLTRE+GPKVV+GDPA K    + T   AH  HH  F+P T  +SDSSLKFTHV
Sbjct: 92  ISASLASLTRESGPKVVRGDPAEKKTDGSTTPAYAHGQHHSIFSPATGAVSDSSLKFTHV 151

Query: 142 LYNLSPAELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKG 201
           LYNLSPAELYEQAI++E+GSF TS GALATLSGAKTGR+PRDKR+V+DA TE ELWWGKG
Sbjct: 152 LYNLSPAELYEQAIKYEKGSFITSNGALATLSGAKTGRAPRDKRVVRDATTEDELWWGKG 211

Query: 202 SPNIEMDEHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHN 261
           SPNIEMDEHTF VNRERAVDYLNSL+KVFVNDQ+LNWDP+N++KVRIVSARAYHSLFMHN
Sbjct: 212 SPNIEMDEHTFMVNRERAVDYLNSLEKVFVNDQYLNWDPENRIKVRIVSARAYHSLFMHN 271

Query: 262 MCIRPTEEELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGE 321
           MCIRPT+EELE+FGTPDFTIYNAGQFPCNR+THYMTSSTS+D+NLARREMVILGTQYAGE
Sbjct: 272 MCIRPTQEELESFGTPDFTIYNAGQFPCNRYTHYMTSSTSVDLNLARREMVILGTQYAGE 331

Query: 322 MKKGLFSLMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDE 381
           MKKGLFS+MHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDE
Sbjct: 332 MKKGLFSVMHYLMPKRRILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDE 391

Query: 382 HCWGDNGVSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVT 441
           HCW + GVSNIEGGCYAKC+DLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSD SVT
Sbjct: 392 HCWTETGVSNIEGGCYAKCVDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDKSVT 451

Query: 442 ENTRAAYPIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL 501
           ENTRAAYPIE+IPNAKIPCVGPHP NVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL
Sbjct: 452 ENTRAAYPIEFIPNAKIPCVGPHPTNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTAL 511

Query: 502 VAGTEDGIKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGS 561
           VAGTEDGIKEP ATFSACFGAAFIMLHPTKYAAMLA+KM+  GATGWLVNTGWSGG YG 
Sbjct: 512 VAGTEDGIKEPTATFSACFGAAFIMLHPTKYAAMLAEKMKSQGATGWLVNTGWSGGSYGV 571

Query: 562 GSRIKLSYTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAY 621
           G+RIKL+YTRKIIDAIHSG LLKANY+KT++FG EIPT IEG+PSEILDP+++WS+K A+
Sbjct: 572 GNRIKLAYTRKIIDAIHSGSLLKANYKKTEIFGFEIPTEIEGIPSEILDPVNSWSDKKAH 631

Query: 622 NETLLKLATLFKNNFETFTNYKI 638
            +TL+KL  LFK NFE F N+KI
Sbjct: 632 KDTLVKLGGLFKKNFEVFANHKI 654

BLAST of Cp4.1LG10g01740 vs. TAIR 10
Match: AT5G65690.1 (phosphoenolpyruvate carboxykinase 2 )

HSP 1 Score: 1026.9 bits (2654), Expect = 6.8e-300
Identity = 499/616 (81.01%), Postives = 548/616 (88.96%), Query Frame = 0

Query: 27  ICHDGSSSPIKAQTIDELHSLQKKRSTPTTP----TGSSPSAFSVLSEEDRQRQQLQSIS 86
           +C D  +  +  QTIDELHSLQKKRS PTTP    + S  S  S  +        LQS+S
Sbjct: 41  VCQDDIAPRVNFQTIDELHSLQKKRSAPTTPLRDGSASGVSGTSGPTTPVSSETMLQSVS 100

Query: 87  ASLASLTRETGPKVVKGDPARKTPTSKIAHV-AHHQFAPTINISDSSLKFTHVLYNLSPA 146
           ASLASLTRETGPK+++GDP   T  +K+AHV       P  ++SDS LKFTH+L+NLSPA
Sbjct: 101 ASLASLTRETGPKLIRGDP---TSAAKVAHVPVTPTSLPAADVSDSGLKFTHILHNLSPA 160

Query: 147 ELYEQAIRHERGSFFTSTGALATLSGAKTGRSPRDKRIVKDANTEKELWWGKGSPNIEMD 206
           ELYEQAI+ E+GSF TSTGALATLSGAKTGRSP+DKR+VKD  TE ELWWGKGSPNIEMD
Sbjct: 161 ELYEQAIKFEKGSFVTSTGALATLSGAKTGRSPKDKRVVKDDTTEAELWWGKGSPNIEMD 220

Query: 207 EHTFTVNRERAVDYLNSLDKVFVNDQFLNWDPQNKLKVRIVSARAYHSLFMHNMCIRPTE 266
           E TF VNRERAVDYLNSLDKVFVNDQ+LNWDP+NK+KVRIVSARAYHSLFMHNMCIRPT 
Sbjct: 221 EKTFLVNRERAVDYLNSLDKVFVNDQYLNWDPENKIKVRIVSARAYHSLFMHNMCIRPTP 280

Query: 267 EELENFGTPDFTIYNAGQFPCNRFTHYMTSSTSIDMNLARREMVILGTQYAGEMKKGLFS 326
           EELENFGTPDFTIYNAG+FPCNRFTHYMTSSTS+D+NL RREMVILGTQYAGEMKKGLF 
Sbjct: 281 EELENFGTPDFTIYNAGKFPCNRFTHYMTSSTSVDINLGRREMVILGTQYAGEMKKGLFG 340

Query: 327 LMHYLMPKRQILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWGDNG 386
           +MHYLMPKR+ILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCW + G
Sbjct: 341 VMHYLMPKRKILSLHSGCNMGKDGDVALFFGLSGTGKTTLSTDHNRYLIGDDEHCWSEAG 400

Query: 387 VSNIEGGCYAKCIDLSREKEPDIWNAIKFGTVLENVVFDEHTREVDYSDNSVTENTRAAY 446
           VSNIEGGCYAKCIDL+R+KEPDIWNAIKFGTVLENVVFDEHTREVDY+D SVTENTRAAY
Sbjct: 401 VSNIEGGCYAKCIDLARDKEPDIWNAIKFGTVLENVVFDEHTREVDYTDKSVTENTRAAY 460

Query: 447 PIEYIPNAKIPCVGPHPKNVILLACDAFGVLPPVSKLNLAQTMYHFISGYTALVAGTEDG 506
           PIEYIPN+KIPCVGPHPKNVILLACDAFGVLPP+SKLNLAQTMYHFISGYTALVAGTE+G
Sbjct: 461 PIEYIPNSKIPCVGPHPKNVILLACDAFGVLPPISKLNLAQTMYHFISGYTALVAGTEEG 520

Query: 507 IKEPQATFSACFGAAFIMLHPTKYAAMLAQKMQKHGATGWLVNTGWSGGRYGSGSRIKLS 566
           +KEP+ATFSACFGAAFIMLHPTKYAAMLA+KMQ  GATGWLVNTGWSGG YG+GSRIKL+
Sbjct: 521 VKEPRATFSACFGAAFIMLHPTKYAAMLAEKMQAQGATGWLVNTGWSGGSYGTGSRIKLA 580

Query: 567 YTRKIIDAIHSGELLKANYRKTKVFGLEIPTAIEGVPSEILDPISTWSNKDAYNETLLKL 626
           YTRKIIDAIHSG LL A+YRKT +FGLEIP  +EGVPSEIL+PI+ W +K AY +TLLKL
Sbjct: 581 YTRKIIDAIHSGSLLNASYRKTDIFGLEIPNEVEGVPSEILEPINAWPDKMAYEDTLLKL 640

Query: 627 ATLFKNNFETFTNYKI 638
           A LFK+NFETFT++KI
Sbjct: 641 AGLFKSNFETFTSHKI 653

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9T0742.8e-31182.99Phosphoenolpyruvate carboxykinase (ATP) 1 OS=Arabidopsis thaliana OX=3702 GN=PCK... [more]
P420665.4e-31082.74Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=PCK PE=2 S... [more]
Q9SLZ06.4e-30380.09Phosphoenolpyruvate carboxykinase (ATP) OS=Zea mays OX=4577 PE=2 SV=1[more]
B5X5749.6e-29981.01Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Arabidopsis thaliana OX=3702 GN=PCK... [more]
Q9XFA27.6e-28879.41Phosphoenolpyruvate carboxykinase (ATP) 2 OS=Urochloa panicoides OX=37563 GN=PCK... [more]
Match NameE-valueIdentityDescription
XP_023544791.10.0100.00phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita pepo subsp. pepo][more]
XP_022950481.10.099.37phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita moschata][more]
KAG6604082.10.099.37Phosphoenolpyruvate carboxykinase (ATP), partial [Cucurbita argyrosperma subsp. ... [more]
KAG7034246.10.099.21Phosphoenolpyruvate carboxykinase (ATP), partial [Cucurbita argyrosperma subsp. ... [more]
XP_022977875.10.098.90phosphoenolpyruvate carboxykinase (ATP)-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GEY40.099.37Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1INJ30.098.90Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita maxima OX=3661 GN=LOC111478... [more]
A0A1S3B0T30.090.79Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis melo OX=3656 GN=LOC103484904 ... [more]
A0A0A0KJU60.089.19Phosphoenolpyruvate carboxykinase (ATP) OS=Cucumis sativus OX=3659 GN=Csa_6G4971... [more]
A0A6J1HFF70.091.04Phosphoenolpyruvate carboxykinase (ATP) OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
Match NameE-valueIdentityDescription
AT4G37870.12.0e-31282.99phosphoenolpyruvate carboxykinase 1 [more]
AT5G65690.16.8e-30081.01phosphoenolpyruvate carboxykinase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingPFAMPF01293PEPCK_ATPcoord: 133..600
e-value: 7.0E-214
score: 710.7
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingTIGRFAMTIGR00224TIGR00224coord: 131..635
e-value: 3.7E-224
score: 743.4
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingPANTHERPTHR30031PHOSPHOENOLPYRUVATE CARBOXYKINASE ATPcoord: 17..637
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingHAMAPMF_00453PEPCK_ATPcoord: 127..641
score: 47.47728
IPR001272Phosphoenolpyruvate carboxykinase, ATP-utilisingCDDcd00484PEPCK_ATPcoord: 132..641
e-value: 0.0
score: 988.261
IPR013035Phosphoenolpyruvate carboxykinase, C-terminalGENE3D3.90.228.20coord: 339..633
e-value: 2.0E-218
score: 728.1
NoneNo IPR availableGENE3D2.170.8.10Phosphoenolpyruvate Carboxykinase, domain 2coord: 152..454
e-value: 2.0E-218
score: 728.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 47..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..35
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..22
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 50..89
NoneNo IPR availablePANTHERPTHR30031:SF10PHOSPHOENOLPYRUVATE CARBOXYKINASE [ATP] PROTEINcoord: 17..637
NoneNo IPR availableSUPERFAMILY53795PEP carboxykinase-likecoord: 332..635
IPR008210Phosphoenolpyruvate carboxykinase, N-terminalGENE3D3.40.449.10Phosphoenolpyruvate Carboxykinase, domain 1coord: 131..338
e-value: 2.0E-218
score: 728.1
IPR008210Phosphoenolpyruvate carboxykinase, N-terminalSUPERFAMILY68923PEP carboxykinase N-terminal domaincoord: 122..330
IPR015994Phosphoenolpyruvate carboxykinase (ATP), conserved sitePROSITEPS00532PEPCK_ATPcoord: 369..384

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g01740.1Cp4.1LG10g01740.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0016310 phosphorylation
cellular_component GO:0005829 cytosol
molecular_function GO:0005524 ATP binding
molecular_function GO:0016301 kinase activity
molecular_function GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
molecular_function GO:0004611 phosphoenolpyruvate carboxykinase activity
molecular_function GO:0017076 purine nucleotide binding