Cp4.1LG10g01480 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG10g01480 vs. ExPASy Swiss-Prot
Match: Q1HFZ0 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Mus musculus OX=10090 GN=Nsun2 PE=1 SV=2)

HSP 1 Score: 417.9 bits (1073), Expect = 2.7e-115
Identity = 272/768 (35.42%), Postives = 399/768 (51.95%), Query Frame = 0

Query: 52  QSHAFNEYYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQ 111
           ++  F  YY+E  IV   EWD F+E LR+ LPA  RI        EI   L+N + K L+
Sbjct: 44  ENKLFEHYYQELKIVPEGEWDQFMESLREPLPATLRITGYKSHAKEILHCLKNKYFKELE 103

Query: 112 SEVTDGDEAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEA 171
               DG +    +PLSWYP+ LAWH+N SR  LRK+  L +FH+FL  E E GNI+RQEA
Sbjct: 104 DLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPLLAKFHQFLVSETESGNISRQEA 163

Query: 172 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCN 231
           VSM+PPL L+V P+H +LDMCAAPGSKT QL+E++H +      P G V+AND D +RC 
Sbjct: 164 VSMIPPLLLNVEPHHKILDMCAAPGSKTTQLIEMLH-ADMSVPFPEGFVIANDVDNKRCY 223

Query: 232 LLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVP 291
           LL+HQ KR+ +  ++V NH+A   P                D++     L +DR+LCDVP
Sbjct: 224 LLVHQAKRLSSPCIMVVNHDASSIPRLTV------------DVDGRKEILFYDRILCDVP 283

Query: 292 CSGDGTLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEA 351
           CSGDGT+RK  D+W++W +     LHGLQ++I  RGA  L  GGRMVYSTCS+NPVE+EA
Sbjct: 284 CSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPVEDEA 343

Query: 352 VVAELLRKSEGSLELVDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIP 411
           V+A LL KSEG+LEL DVS ELP L   PG+ +WKV  R G W A + +V          
Sbjct: 344 VIAALLEKSEGALELADVSAELPGLKWMPGVSQWKVMTRDGQWFADWHEV---------- 403

Query: 412 SMFPSGRGRKNQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMR 471
              P GR          ++++R                PTM P  + +++    +ERC+R
Sbjct: 404 ---PQGR----------HTQIR----------------PTMFPPTDLEKLQAMHLERCLR 463

Query: 472 IVPHDQNSGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSAS 531
           I+PH QN+G FF+AVL K AP+P    +P  QN      + R     + T G   D S  
Sbjct: 464 ILPHHQNTGGFFVAVLVKKAPMPWNKRQPKVQNKSAEAREPRVSSHVAATEGNPSDQSEL 523

Query: 532 VGMDESPSEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLE 591
               ES    G      GD  +A N        ++ E  E  DG       K        
Sbjct: 524 ----ESQMITGA-----GDSETAHN-------TENTESNEKKDGVCGPPPSK-------- 583

Query: 592 VNLVDNEVSVNNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLF-KYETVIDSIK 651
                                           K+++ G  +  DP V   + + +   I+
Sbjct: 584 --------------------------------KMKLFGFKE--DPFVFIPEDDPLFPPIE 643

Query: 652 TFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFE 711
            FY ++ +FP   +L+TR T+    +++Y VSK +++VL  N    +++K+ + G+K++ 
Sbjct: 644 KFYALDPSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWC 689

Query: 712 RQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFG 771
           R +S  G    C+FR+  EG+  + P+I  +I+T+S  D K L+  ++  +   +  A+ 
Sbjct: 704 RNNS--GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKTLLTQENPFFRKLSSEAYS 689

Query: 772 EKASNLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 818
           +   +L  G  V++     + + D +   +  I +  W+G++S+   V
Sbjct: 764 Q-VKDLAKG-SVVLKYEPDSANPDTL---QCPIVLCGWRGKASIRTFV 689

BLAST of Cp4.1LG10g01480 vs. ExPASy Swiss-Prot
Match: Q5ZLV4 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Gallus gallus OX=9031 GN=NSUN2 PE=2 SV=1)

HSP 1 Score: 412.5 bits (1059), Expect = 1.1e-113
Identity = 271/816 (33.21%), Postives = 406/816 (49.75%), Query Frame = 0

Query: 7   GGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYYKEQGIV 66
           G RAR +R   +  +    +          D + G +  +     ++  F  YY+EQ IV
Sbjct: 2   GRRARDRRRQLQPQQR---RERSGGGGGGGDDQAGWAGGYPEIVKENELFERYYREQRIV 61

Query: 67  TAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEAGAIRPL 126
              EWD F+  LR+ LPA  RI        EI   L+  + + LQ    DG +    + L
Sbjct: 62  PDGEWDAFMAALREPLPATLRITGYKSHAREILHCLKEKYFRELQHLEVDGQKVEMPQAL 121

Query: 127 SWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNH 186
           SWYP+ LAWH+N SR  LRK+  LERFH+FL  E E GNI+RQEAVSM+PPL L+V+P+H
Sbjct: 122 SWYPEELAWHTNLSRKILRKSPQLERFHQFLVSETECGNISRQEAVSMIPPLLLNVNPDH 181

Query: 187 YVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRMCTSNLI 246
            +LDMCAAPGSKT QL+E++H        P G V+AND D +RC LL+HQ KR+ +  ++
Sbjct: 182 KILDMCAAPGSKTAQLIEMLHADMNV-PFPKGFVIANDVDNKRCYLLVHQAKRLNSPCIM 241

Query: 247 VTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRKAPDIWR 306
           V NH+A   P  +             D++     L +DR+LCDVPCSGDGT+RK  D+W+
Sbjct: 242 VVNHDASSIPNLQV------------DVDGRKETLFYDRILCDVPCSGDGTMRKNIDVWK 301

Query: 307 RWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSEGSLEL 366
           +W +     LHGLQ++I  RG   L  GGRMVYSTCS+NP+ENEAV+A LL KS+G+LEL
Sbjct: 302 KWTTQNSLQLHGLQLRIATRGVEQLAEGGRMVYSTCSLNPIENEAVIASLLEKSQGALEL 361

Query: 367 VDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPSGRGRKNQADK 426
            DVS+ELP L R PG+ KWKV  + G W   +K V  +RQ+   P+MF            
Sbjct: 362 ADVSSELPGLKRMPGITKWKVMLKDGQWFEEWKDVPSNRQTQIRPTMF------------ 421

Query: 427 SHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFFIAV 486
                                      P+ E +++    +ERC+RI+PH QN+G FF+AV
Sbjct: 422 ---------------------------PIKEEEKLKAMNLERCIRILPHHQNTGGFFVAV 481

Query: 487 LRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEVGVDSN 546
           L K +P+P    +P      P                                       
Sbjct: 482 LIKKSPMPWNKRQPKVHQKLP--------------------------------------- 541

Query: 547 NQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSVNNLEI 606
                                E TE    NA DG +    +A  +  L ++E      E+
Sbjct: 542 ------------------GKTEDTEVTATNAGDGSE----DATEKPTLAEDEEPKKVQEL 601

Query: 607 TAIEDNLEESKQSTV----KRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPLF 666
                + E+SK+        +K+++ G +K    V L + + +   I+ FY ++ +FP  
Sbjct: 602 Q--NSDTEQSKKGVCGPPPSKKMKLFG-FKEDPFVFLPEDDPLFLPIQKFYALDPSFPKM 661

Query: 667 GHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAPC 726
            +L+TR T     +++Y VSK +++VL  N    +++K+ + G+K++ R S  +G    C
Sbjct: 662 -NLLTR-TQEGKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWSRNS--DGEQFGC 686

Query: 727 SFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCCV 786
           +FR+  EG+  + P+I  +I+ +   D K L+  ++   + F+      K  ++ +G  V
Sbjct: 722 AFRLAQEGIYTLYPFIHARIVNVCIEDVKILLTQENPFLSKFSSET-QRKVKDMAMGSIV 686

Query: 787 IVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 818
           +          D+    +  I +  W+G++SL   V
Sbjct: 782 LKYEPDPEKPDDL----QCPIVLCGWQGKTSLRAFV 686

BLAST of Cp4.1LG10g01480 vs. ExPASy Swiss-Prot
Match: Q08J23 (RNA cytosine C(5)-methyltransferase NSUN2 OS=Homo sapiens OX=9606 GN=NSUN2 PE=1 SV=2)

HSP 1 Score: 410.2 bits (1053), Expect = 5.5e-113
Identity = 276/822 (33.58%), Postives = 412/822 (50.12%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQ---SQSHAFN 60
           MG + RG R + Q+           +    +  +    K G +   G +     ++  F 
Sbjct: 1   MGRRSRGRRLQQQQ-----------RPEDAEDGAEGGGKRGEAGWEGGYPEIVKENKLFE 60

Query: 61  EYYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDG 120
            YY+E  IV   EW  F++ LR+ LPA  RI        EI   L+N + K L+    DG
Sbjct: 61  HYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKELEDLEVDG 120

Query: 121 DEAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPP 180
            +    +PLSWYP+ LAWH+N SR  LRK+  LE+FH+FL  E E GNI+RQEAVSM+PP
Sbjct: 121 QKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISRQEAVSMIPP 180

Query: 181 LFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQT 240
           L L+V P+H +LDMCAAPGSKT QL+E++H        P G V+AND D +RC LL+HQ 
Sbjct: 181 LLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQA 240

Query: 241 KRMCTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGT 300
           KR+ +  ++V NH+A   P  +             D++     L +DR+LCDVPCSGDGT
Sbjct: 241 KRLSSPCIMVVNHDASSIPRLQI------------DVDGRKEILFYDRILCDVPCSGDGT 300

Query: 301 LRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELL 360
           +RK  D+W++W +     LHGLQ++I  RGA  L  GGRMVYSTCS+NP+E+EAV+A LL
Sbjct: 301 MRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIASLL 360

Query: 361 RKSEGSLELVDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPSG 420
            KSEG+LEL DVSNELP L   PG+ +WKV  + G W   +  V   R +   P+MFP  
Sbjct: 361 EKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTMFPPK 420

Query: 421 RGRKNQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQ 480
              K QA                                         +ERC+RI+PH Q
Sbjct: 421 DPEKLQA---------------------------------------MHLERCLRILPHHQ 480

Query: 481 NSGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDES 540
           N+G FF+AVL K + +P    +P  Q       +S    P  +T G   D S      ES
Sbjct: 481 NTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKL----ES 540

Query: 541 PSEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDN 600
           PS  G      GD   A          +D+E     +G+  DG+                
Sbjct: 541 PSFTGT-----GDTEIA-------HATEDLEN----NGSKKDGV---------------- 600

Query: 601 EVSVNNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLF-KYETVIDSIKTFYGIN 660
                                    +K+++ G  +  DP V   + + +   I+ FY ++
Sbjct: 601 -------------------CGPPPSKKMKLFGFKE--DPFVFIPEDDPLFPPIEKFYALD 660

Query: 661 EAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSRE 720
            +FP   +L+TR T+    +++Y VSK +++VL  N    +++K+ + G+K++ R +S  
Sbjct: 661 PSFPRM-NLLTRTTE-GKKRQLYMVSKELRNVLLNN---SEKMKVINTGIKVWCRNNS-- 690

Query: 721 GSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNL 780
           G    C+FR+  EG+  + P+I  +I+T+S  D K L+  ++  +   +   + + A +L
Sbjct: 721 GEEFDCAFRLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQ-AKDL 690

Query: 781 MLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 818
             G  +++     + + D +   +  I +  W+G++S+   V
Sbjct: 781 AKG-SIVLKYEPDSANPDAL---QCPIVLCGWRGKASIRTFV 690

BLAST of Cp4.1LG10g01480 vs. ExPASy Swiss-Prot
Match: Q28E61 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus tropicalis OX=8364 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.6e-110
Identity = 277/821 (33.74%), Postives = 409/821 (49.82%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MG + R  R R QR+  + S     +R K    +A +        +     ++  F  YY
Sbjct: 1   MGRRNRRNRQRHQRSTEQRSPAEEEQRRKAREQAAWEC------GYPEIIKENKLFEHYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           +E  IV   EWD F+  LR+ LPA  RI        EI   L+  + K LQ    DG + 
Sbjct: 61  QELKIVPDGEWDKFMASLREPLPATIRITGYKSHAKEILHCLKEKYFKELQDIEVDGQKI 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
            A +PLSWYP+ LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+PP+ L
Sbjct: 121 EAPQPLSWYPEELAWHTNLSRKIIRKSPELEKFHQFLVNETESGNISRQEAVSMIPPVLL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
            V P+H +LDMCAAPGSKT Q++E++H        P G V+AND D +RC LL+HQ KR+
Sbjct: 181 KVQPHHKILDMCAAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAKRL 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
            +  ++V NH+A   P      N       GS        L +DR+LCDVPCSGDGTLRK
Sbjct: 241 NSPCIMVVNHDASSIPRLLVENN-------GS-----REVLYYDRILCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
             D+W++W +     LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+A LL KS
Sbjct: 301 NIDVWKKWTTLNSLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIASLLDKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPSGRGR 420
           EGSLEL DV++E+P L   PG+ +WKV  + G W   ++ +   R +   P+MFP    +
Sbjct: 361 EGSLELADVASEIPGLKWMPGITQWKVMTKEGHWYEKWEDIPTSRHTQIRPTMFPP---K 420

Query: 421 KNQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSG 480
             +  KS N                                    + RCMRI+PH QN+G
Sbjct: 421 DEEKLKSMN------------------------------------LNRCMRILPHHQNTG 480

Query: 481 AFFIAVLRKVAPLPAATEKPSYQNLKPVN--DKSRCKPPQSITNGANVDVSASVGMDESP 540
            FF+AVL K AP+P    +P  Q   PV+  D S    P+ +          S GM + P
Sbjct: 481 GFFVAVLIKKAPMPWNKRQPKLQRRPPVSACDASIAVAPELV----KAVTENSAGMADEP 540

Query: 541 SEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNE 600
           +   VD+ N       + KP                                      N+
Sbjct: 541 A---VDTEN------GETKPC------------------------------------TNQ 600

Query: 601 VSVNNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDP-VVLFKYETVIDSIKTFYGINE 660
              +  +I       ++ K    K            DP V + + + + D I+TFY ++ 
Sbjct: 601 SDSSKTDIVCCPPPSKKMKLFGFKE-----------DPFVFVSEDDPIFDPIQTFYALDP 660

Query: 661 AFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREG 720
           +FP   +L+TR T     +++Y VSK +++VL  N    +++K+ + G+K+  R  + +G
Sbjct: 661 SFPK-KNLLTR-TQEGKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKVLCR--NNDG 691

Query: 721 SSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLM 780
               C++R+  EG+  + P+I  +I+T+S  D K L+  ++   + F+     ++A+N  
Sbjct: 721 EQYGCAYRLAQEGIYTLYPFINARIVTVSIEDIKVLLTQENPFLSKFSKET-QKQANNFD 691

Query: 781 LGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMV 818
           +G  V+           +    +  I +  W+G++S+   V
Sbjct: 781 MGSIVLKYEPDPQEPETL----QCPIVLCGWRGKTSIRSFV 691

BLAST of Cp4.1LG10g01480 vs. ExPASy Swiss-Prot
Match: Q4V7N2 (RNA cytosine-C(5)-methyltransferase NSUN2 OS=Xenopus laevis OX=8355 GN=nsun2 PE=2 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 3.7e-109
Identity = 262/807 (32.47%), Postives = 406/807 (50.31%), Query Frame = 0

Query: 18  RESRENVWKRSKPDSNSANDTKDGN-----SNHWGPFQSQSHAFNEYYKEQGIVTAEEWD 77
           R++R N  +R++  S +  + +           +     ++  F  YY+E  IV   EWD
Sbjct: 3   RKNRRNRQRRTEQRSPAEEERRKAREQAAWEGGYPEIIKENKLFEHYYQELKIVPDGEWD 62

Query: 78  TFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEAGAIRPLSWYPDN 137
            F++ LR+ LPA  RI        EI   L+  + K L     DG +  A +PLSWYP+ 
Sbjct: 63  KFMDALREPLPATIRITGYKSHAKEILHCLKEKYFKELPDIEVDGQKIEAPQPLSWYPEE 122

Query: 138 LAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFLDVHPNHYVLDMC 197
           LAWH+N SR  +RK+  LE+FH+FL  E E GNI+RQEAVSM+PP+ L+V P+H +LDMC
Sbjct: 123 LAWHTNLSRKIIRKSPELEKFHQFLVSETESGNISRQEAVSMIPPVLLNVQPHHKILDMC 182

Query: 198 AAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRMCTSNLIVTNHEA 257
           AAPGSKT Q++E++H        P G V+AND D +RC LL+HQ KR+ +  ++V NH+A
Sbjct: 183 AAPGSKTAQIIEMLHADMNV-PFPEGFVIANDVDNKRCYLLVHQAKRLNSPCIMVVNHDA 242

Query: 258 QHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRKAPDIWRRWNSGV 317
              P      N       GS        L +DR+LCDVPCSGDGT+RK  D+W++W +  
Sbjct: 243 SSIPRLLIENN-------GS-----REVLYYDRILCDVPCSGDGTMRKNIDVWKKWTTLN 302

Query: 318 GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKSEGSLELVDVSNE 377
              LHGLQ++I  RG   L  GGRMVYSTCS+NPVE+EAV+  LL KSEGSLEL DV++E
Sbjct: 303 SLQLHGLQIRIATRGVEQLAEGGRMVYSTCSLNPVEDEAVIVSLLDKSEGSLELADVASE 362

Query: 378 LPQLVRRPGLKKWKVRDR-GVWLASYKQVSDHRQSVAIPSMFPSGRGRKNQADKSHNSEL 437
           LP L   PG+ +W+V  + G W   ++ V                         S ++++
Sbjct: 363 LPGLKWMPGITQWRVMTKEGQWFEKWEDV-----------------------PTSRHTQI 422

Query: 438 RGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGAFFIAVLRKVAP 497
           R                PTM P+ + +++    + RCMRI+PH QN+G FF+AVL K AP
Sbjct: 423 R----------------PTMFPLKDEEKLKSMNLNRCMRILPHHQNTGGFFVAVLIKKAP 482

Query: 498 LPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVD-VSASVGMDESPSEVGVDSNNQGDF 557
           +P    +P  Q   PV+       P+ +   A++  ++    +D    E    +N  G  
Sbjct: 483 MPWNKRQPKLQRRPPVSVCDASVAPEIVKAVADISAIADEPAVDAENGETKPCTNQSG-- 542

Query: 558 NSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSVNNLEITAIED 617
           +S  +   C  P                                                
Sbjct: 543 SSKTDSVCCPPP------------------------------------------------ 602

Query: 618 NLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPLFGHLVTRNTD 677
                      +K+++ G +K    V L + + + + I+ FY ++ +FP   +L+TR T 
Sbjct: 603 ----------SKKMKLFG-FKEDPFVFLSEDDPIFEPIQKFYALDPSFPK-KNLLTR-TQ 662

Query: 678 TNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAPCSFRITSEGL 737
               +++Y VSK +++VL  N    +++K+ + G+K+  R  + +G    C++R+  EG+
Sbjct: 663 EGKKRQLYMVSKELRNVLLHN---SEKMKVINTGIKVLCR--NNDGEQYGCAYRLAQEGI 684

Query: 738 PVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCCVIVLGTGGNT 797
             + P+I  +ILT+S  D K L+  ++   + F+     ++A+NL +G  V+        
Sbjct: 723 YSLYPFINARILTVSVEDIKVLLTQENPFLSKFSKET-QKQANNLDMGSIVLKYEPDPQQ 684

Query: 798 SSDVIKVDESTIAIGCWKGRSSLSVMV 818
              +    +  I +  W+G++S+   V
Sbjct: 783 PETL----QCPIVLCGWRGKTSIRSFV 684

BLAST of Cp4.1LG10g01480 vs. NCBI nr
Match: XP_023543635.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1680 bits (4351), Expect = 0.0
Identity = 837/837 (100.00%), Postives = 837/837 (100.00%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL 660
           NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL
Sbjct: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL 660

Query: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP 720
           FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP
Sbjct: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP 720

Query: 721 CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780
           CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC
Sbjct: 721 CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780

Query: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837
           VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV
Sbjct: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837

BLAST of Cp4.1LG10g01480 vs. NCBI nr
Match: XP_022950405.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1652 bits (4277), Expect = 0.0
Identity = 823/837 (98.33%), Postives = 828/837 (98.92%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTNI NASAAGS+LNPHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHLDGSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLD NAADGIDKQFSEADLEVNLVDNEVSV
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL 660
           NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLF+ ETVIDSIKTFYGINEAFPL
Sbjct: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIKTFYGINEAFPL 660

Query: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP 720
           FGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NF VGQQLKITSIGLKMFERQSSREGS AP
Sbjct: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFERQSSREGSCAP 720

Query: 721 CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780
           CSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGEKASNLMLGCC
Sbjct: 721 CSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780

Query: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837
           VIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV
Sbjct: 781 VIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837

BLAST of Cp4.1LG10g01480 vs. NCBI nr
Match: XP_022950404.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1644 bits (4256), Expect = 0.0
Identity = 823/847 (97.17%), Postives = 828/847 (97.76%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTNI NASAAGS+LNPHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHLDGSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLD NAADGIDKQFSEADLEVNLVDNEVSV
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKYETVIDSIKT 660
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLF+ ETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIKT 660

Query: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFER 720
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NF VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFER 720

Query: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGE 780
           QSSREGS APCSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 781 KASNLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837
           KASNLMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

BLAST of Cp4.1LG10g01480 vs. NCBI nr
Match: XP_022978422.1 (tRNA (cytosine(34)-C(5))-methyltransferase-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1642 bits (4252), Expect = 0.0
Identity = 816/837 (97.49%), Postives = 826/837 (98.69%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTN  NASAAGS+LN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHL+GSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLD NAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL 660
           NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFK ETVIDSIKTFYGINEAFPL
Sbjct: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKTFYGINEAFPL 660

Query: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP 720
           FGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NFSVGQQLKITSIGLKMFERQSSREGSSAP
Sbjct: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFERQSSREGSSAP 720

Query: 721 CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780
           CSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGEKAS+LMLGCC
Sbjct: 721 CSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGEKASSLMLGCC 780

Query: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837
           VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV
Sbjct: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837

BLAST of Cp4.1LG10g01480 vs. NCBI nr
Match: KAG6604044.1 (RNA cytosine C(5)-methyltransferase NSUN2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034210.1 tRNA (cytosine(34)-C(5))-methyltransferase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1638 bits (4241), Expect = 0.0
Identity = 820/847 (96.81%), Postives = 827/847 (97.64%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVH NHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHRNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTN  NASAAG +LN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGIELNHHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHLDGSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           G+DSNNQGDFNSADNKPACEFPVQDI+GTEGLD NAADGIDKQFSEADLEVNLVDNEVSV
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIQGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKYETVIDSIKT 660
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLFK ETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFER 720
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NF+VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFAVGQQLKITSIGLKMFER 720

Query: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGE 780
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 781 KASNLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837
           KASNLMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

BLAST of Cp4.1LG10g01480 vs. ExPASy TrEMBL
Match: A0A6J1GFM2 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1652 bits (4277), Expect = 0.0
Identity = 823/837 (98.33%), Postives = 828/837 (98.92%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTNI NASAAGS+LNPHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHLDGSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLD NAADGIDKQFSEADLEVNLVDNEVSV
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL 660
           NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLF+ ETVIDSIKTFYGINEAFPL
Sbjct: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIKTFYGINEAFPL 660

Query: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP 720
           FGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NF VGQQLKITSIGLKMFERQSSREGS AP
Sbjct: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFERQSSREGSCAP 720

Query: 721 CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780
           CSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGEKASNLMLGCC
Sbjct: 721 CSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780

Query: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837
           VIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV
Sbjct: 781 VIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837

BLAST of Cp4.1LG10g01480 vs. ExPASy TrEMBL
Match: A0A6J1GFP9 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453515 PE=3 SV=1)

HSP 1 Score: 1644 bits (4256), Expect = 0.0
Identity = 823/847 (97.17%), Postives = 828/847 (97.76%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTA EWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAAEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTNI NASAAGS+LNPHNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNIFNASAAGSELNPHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK
Sbjct: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHLDGSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLDGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKLPQTITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLD NAADGIDKQFSEADLEVNLVDNEVSV
Sbjct: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDSNAADGIDKQFSEADLEVNLVDNEVSV 600

Query: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKYETVIDSIKT 660
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLF+ ETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFRDETVIDSIKT 660

Query: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFER 720
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NF VGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFLVGQQLKITSIGLKMFER 720

Query: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGE 780
           QSSREGS APCSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSCAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 781 KASNLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837
           KASNLMLGCCVIVLGTG NTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASNLMLGCCVIVLGTGSNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

BLAST of Cp4.1LG10g01480 vs. ExPASy TrEMBL
Match: A0A6J1IL24 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1642 bits (4252), Expect = 0.0
Identity = 816/837 (97.49%), Postives = 826/837 (98.69%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTN  NASAAGS+LN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHL+GSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLD NAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGINEAFPL 660
           NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFK ETVIDSIKTFYGINEAFPL
Sbjct: 601 NNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKTFYGINEAFPL 660

Query: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREGSSAP 720
           FGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NFSVGQQLKITSIGLKMFERQSSREGSSAP
Sbjct: 661 FGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFERQSSREGSSAP 720

Query: 721 CSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLMLGCC 780
           CSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGEKAS+LMLGCC
Sbjct: 721 CSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGEKASSLMLGCC 780

Query: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837
           VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV
Sbjct: 781 VIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNRLGNDV 837

BLAST of Cp4.1LG10g01480 vs. ExPASy TrEMBL
Match: A0A6J1IQ20 (tRNA (Cytosine(34)-C(5))-methyltransferase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111478413 PE=3 SV=1)

HSP 1 Score: 1634 bits (4231), Expect = 0.0
Identity = 816/847 (96.34%), Postives = 826/847 (97.52%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60
           MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY
Sbjct: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 60

Query: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120
           KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA
Sbjct: 61  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 120

Query: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180
           GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL
Sbjct: 121 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 180

Query: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240
           DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM
Sbjct: 181 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 240

Query: 241 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 300
           CTSNLIVTNHEAQHFPGCRAHTN  NASAAGS+LN HNTQLTFDRVLCDVPCSGDGTLRK
Sbjct: 241 CTSNLIVTNHEAQHFPGCRAHTNFFNASAAGSELNSHNTQLTFDRVLCDVPCSGDGTLRK 300

Query: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360
           APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS
Sbjct: 301 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 360

Query: 361 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 420
           EGSLE+VDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFP GRGRK
Sbjct: 361 EGSLEVVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPFGRGRK 420

Query: 421 NQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480
           NQADKSHNSELRGNHL+GSIGSS DVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA
Sbjct: 421 NQADKSHNSELRGNHLNGSIGSSVDVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSGA 480

Query: 481 FFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSEV 540
           FFIAVLRKVAPLPAATEKPSY NLKP NDKSRCK PQ+ITNGANVDVSASVGMDESPSEV
Sbjct: 481 FFIAVLRKVAPLPAATEKPSYHNLKPANDKSRCKLPQNITNGANVDVSASVGMDESPSEV 540

Query: 541 GVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVSV 600
           G+DSNNQGDFNSADNKPACEFPVQDIEG EGLD NAADGIDKQFSEADLEVNL+DNEV+V
Sbjct: 541 GIDSNNQGDFNSADNKPACEFPVQDIEGKEGLDSNAADGIDKQFSEADLEVNLIDNEVNV 600

Query: 601 NNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKYETVIDSIKT 660
           NNLEITAIEDNLEESKQ          STVKRKLQIQGKWKGVDPVVLFK ETVIDSIKT
Sbjct: 601 NNLEITAIEDNLEESKQPDDGEMDPKKSTVKRKLQIQGKWKGVDPVVLFKDETVIDSIKT 660

Query: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFER 720
           FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLE+NFSVGQQLKITSIGLKMFER
Sbjct: 661 FYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLELNFSVGQQLKITSIGLKMFER 720

Query: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGE 780
           QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSP+DFKHLIQHKSIKYADFADAAFGE
Sbjct: 721 QSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPVDFKHLIQHKSIKYADFADAAFGE 780

Query: 781 KASNLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 837
           KAS+LMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS
Sbjct: 781 KASSLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLS 840

BLAST of Cp4.1LG10g01480 vs. ExPASy TrEMBL
Match: A0A6J1BXA3 (tRNA (Cytosine(34)-C(5))-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111005554 PE=3 SV=1)

HSP 1 Score: 1392 bits (3603), Expect = 0.0
Identity = 701/850 (82.47%), Postives = 762/850 (89.65%), Query Frame = 0

Query: 1   MGGKGRGGRARTQRNHFRESRENVWKRSKPDSNSANDTKDGN--SNHWGPFQSQSHAFNE 60
           MGGKGRGGRARTQR HFRE+RENVWKR +PD NS ND KDGN  +N W PF SQS AFNE
Sbjct: 1   MGGKGRGGRARTQRKHFRENRENVWKRPRPDPNSGNDNKDGNGNTNPWEPFDSQSPAFNE 60

Query: 61  YYKEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGD 120
           YYKEQGIVTAEEWDTFIEVLRK LPAAFRINSSGQFCGEIRAQLQNDFMKSL++EV DGD
Sbjct: 61  YYKEQGIVTAEEWDTFIEVLRKPLPAAFRINSSGQFCGEIRAQLQNDFMKSLKAEVADGD 120

Query: 121 EAGAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180
           EA AIRPL WYP+NLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL
Sbjct: 121 EAEAIRPLPWYPENLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPL 180

Query: 181 FLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTK 240
           FLDVHP+HYVLDMCAAPGSKTFQLLEIIH SSKPG LP GLVVAND DVQRCNLLIHQTK
Sbjct: 181 FLDVHPSHYVLDMCAAPGSKTFQLLEIIHLSSKPGGLPEGLVVANDLDVQRCNLLIHQTK 240

Query: 241 RMCTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTL 300
           RMCT+NLIVTNHEAQHFPGCRA+ N  NASAAG++L P N+QL+FDRVLCDVPCSGDGTL
Sbjct: 241 RMCTANLIVTNHEAQHFPGCRANINFFNASAAGNELKPQNSQLSFDRVLCDVPCSGDGTL 300

Query: 301 RKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360
           RKAPDIWR+WN+G+GNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR
Sbjct: 301 RKAPDIWRKWNAGMGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLR 360

Query: 361 KSEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRG 420
           KS+GSLELVDVSNELPQLVRR GLKKW+VRD+GVWLASYKQV++ RQSV IPSMFPSGR 
Sbjct: 361 KSQGSLELVDVSNELPQLVRRSGLKKWRVRDKGVWLASYKQVNECRQSVVIPSMFPSGRS 420

Query: 421 RKNQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDE-VCEFPIERCMRIVPHDQN 480
           RKNQA+ +HNSEL    LDGS GSS DV++  +DP+HEFDE VCEFP+ERCMRIVPHDQN
Sbjct: 421 RKNQAEDNHNSELGETRLDGSTGSSVDVADAAVDPLHEFDEEVCEFPLERCMRIVPHDQN 480

Query: 481 SGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESP 540
           SGAFFIAVL KV+PLPAATEKP  Q  K  ND+S CK PQ+IT+  +VD++A  GMDESP
Sbjct: 481 SGAFFIAVLHKVSPLPAATEKPINQKSKSANDRSHCKLPQAITDVDSVDINAIDGMDESP 540

Query: 541 SEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNE 600
           SEV +D N QGD+NSAD K   + PV+D++  +GLD +AADG+DKQ SEA LE NL+DNE
Sbjct: 541 SEVEIDLNKQGDYNSADEKSPSKRPVEDVKDDDGLDSSAADGMDKQLSEAGLEDNLIDNE 600

Query: 601 VSVNNLEITAIEDNLEESKQ----------STVKRKLQIQGKWKGVDPVVLFKYETVIDS 660
           VS NNLEITAI+D  EESK+          STVKRKLQIQGKWKGVDPVVLFK ETVIDS
Sbjct: 601 VSENNLEITAIKDKAEESKEADDGGTDPNKSTVKRKLQIQGKWKGVDPVVLFKDETVIDS 660

Query: 661 IKTFYGINEAFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKM 720
           IKTFYGINE+FPL GHLVTRN+DTNHVKRIYY+SKSVKDVLE+NFSVGQQLKITSIGLKM
Sbjct: 661 IKTFYGINESFPLIGHLVTRNSDTNHVKRIYYISKSVKDVLELNFSVGQQLKITSIGLKM 720

Query: 721 FERQSSREGSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAA 780
           FERQSSREGSSAPCSFRI+SEGLPVILPYITKQILT+SP+DFKHL+ ++SIKYADF DAA
Sbjct: 721 FERQSSREGSSAPCSFRISSEGLPVILPYITKQILTISPVDFKHLLIYESIKYADFVDAA 780

Query: 781 FGEKASNLMLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLE 837
           FGEKASNLMLGCCVIVLG G N SSDVIKVDESTIAIGCWKGR+SLSVM+TAID QELL 
Sbjct: 781 FGEKASNLMLGCCVIVLGKGSNPSSDVIKVDESTIAIGCWKGRASLSVMLTAIDRQELLG 840

BLAST of Cp4.1LG10g01480 vs. TAIR 10
Match: AT2G22400.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 900.2 bits (2325), Expect = 1.3e-261
Identity = 482/836 (57.66%), Postives = 591/836 (70.69%), Query Frame = 0

Query: 5   GRGG--RARTQRNHFRESRENVWKRSKPDS--NSANDTKDGNSNHWGPFQSQSHAFNEYY 64
           GRGG  R RTQR  F+ESRENVWKR K D+  + +++        W P  + +  F EYY
Sbjct: 2   GRGGRHRGRTQRKDFKESRENVWKRPKSDASVDGSDNAVPEQKPTWEPIVTVNPNFEEYY 61

Query: 65  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 124
           K+QGIV AEEWD F+E+LRK LPAAFR+NS+GQFC EI + L+NDFMKSLQ+E  +G E 
Sbjct: 62  KKQGIVKAEEWDLFMEILRKPLPAAFRVNSNGQFCDEIISILENDFMKSLQAEAIEGGEL 121

Query: 125 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 184
            AI+PL WYP NLAWHSNFSR ++RKNQ LERFHEFLKLENE+GNITRQEAVSMVPPLFL
Sbjct: 122 EAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLENEVGNITRQEAVSMVPPLFL 181

Query: 185 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 244
           DVHP+H+VLDMCAAPGSKTFQLLEIIH++S+PG LP GLVVAND D +R NLLIHQTKRM
Sbjct: 182 DVHPDHFVLDMCAAPGSKTFQLLEIIHEASEPGSLPNGLVVANDVDFKRSNLLIHQTKRM 241

Query: 245 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLCDVPCSGDGTLRK 304
           CTSNLIVTNHE Q FPGCR   N   AS  G   N    QL FDRVLCDVPCSGDGTLRK
Sbjct: 242 CTSNLIVTNHEGQQFPGCR--LNKSRASEKGISENMPINQLAFDRVLCDVPCSGDGTLRK 301

Query: 305 APDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRKS 364
           APDIWR+WNSG+GNGLH LQ+ + MRG SLLKVGG+M+YSTCSMNPVE+EAVVAE+LR+ 
Sbjct: 302 APDIWRKWNSGMGNGLHSLQIILAMRGLSLLKVGGKMIYSTCSMNPVEDEAVVAEILRRC 361

Query: 365 EGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGRK 424
             S+EL+DVS++LP+L+RRPGLK WKVRD+G W  SYK V  +R+   + SMFPSG+  K
Sbjct: 362 GDSVELLDVSDKLPELIRRPGLKAWKVRDKGGWFTSYKDVPQNRRGGVLVSMFPSGKYLK 421

Query: 425 NQ---ADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQN 484
           +     +K+ N ++ G   DG      D+S     P  + +EV + P+ERCMRI+PHDQN
Sbjct: 422 DSTETTEKNENGDVNGCE-DGL--KETDISVVDATPEEQAEEVSDLPLERCMRIIPHDQN 481

Query: 485 SGAFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESP 544
           +GAFFIAVL+K +PLP   EKP+ +  +    KS     +S +  + V V A V     P
Sbjct: 482 TGAFFIAVLQKKSPLPEFQEKPNTK--RNSTAKSTDSTEKSPSKESVVTVDAGV-----P 541

Query: 545 SEVGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNE 604
            E  V+   + D N              IE  + L+       +K+ +E +         
Sbjct: 542 DESAVEKVIEADSN--------------IEKNDSLEP------EKKITEGE--------- 601

Query: 605 VSVNNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGI-NE 664
                   +  ED    S  +  KRK+ +QGKWKG DPVV  K ETVI+ IK FYGI +E
Sbjct: 602 --------SITEDKEANSSNAGGKRKVPMQGKWKGFDPVVFVKDETVINGIKEFYGIKDE 661

Query: 665 AFPLFGHLVTRNTDTNHVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSREG 724
           +FPL GHLV RNTDT+ VKRIYYVSKSVK+VL++NF+VGQQLKI S+GLKMFERQS++EG
Sbjct: 662 SFPLHGHLVARNTDTSSVKRIYYVSKSVKEVLQLNFAVGQQLKIASVGLKMFERQSAKEG 721

Query: 725 SSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNLM 784
           SS  C FRI+SEGLPVILPYITKQ+L     DFK L+Q KSIK+ DF +    +KA++L+
Sbjct: 722 SSTLCPFRISSEGLPVILPYITKQVLYTPMADFKLLLQDKSIKFHDFVNPQLAQKATDLV 781

Query: 785 LGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNR 833
           +G CV++L  G       +K D STIAI CW+G++SL+VM T  DCQELLER + +
Sbjct: 782 MGSCVVILSDG----EVPVKADASTIAISCWRGKASLAVMATVADCQELLERFAEK 784

BLAST of Cp4.1LG10g01480 vs. TAIR 10
Match: AT4G40000.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 826.6 bits (2134), Expect = 1.8e-239
Identity = 452/837 (54.00%), Postives = 570/837 (68.10%), Query Frame = 0

Query: 3   GKGRGGRART-QRNHFRESR-ENVWKRSKPDSNSANDTKDGNSNHWGPFQSQSHAFNEYY 62
           G+GR  R  T +RN    S  +NV K  K +++        ++N   P  S  H F EYY
Sbjct: 2   GRGRRHRGSTSKRNQIENSNDDNVRKPLKSEASF------DSNNLQKPICSNLH-FEEYY 61

Query: 63  KEQGIVTAEEWDTFIEVLRKQLPAAFRINSSGQFCGEIRAQLQNDFMKSLQSEVTDGDEA 122
           KEQ IV A+EWD+F+E LRK LPAAFR+ S+GQFC EIR +L+NDFM SLQ+E  +  E 
Sbjct: 62  KEQRIVKAQEWDSFMEFLRKPLPAAFRVYSNGQFCDEIRLKLENDFMISLQAEAIESGEL 121

Query: 123 GAIRPLSWYPDNLAWHSNFSRMQLRKNQALERFHEFLKLENEIGNITRQEAVSMVPPLFL 182
            AI+PL WYP NLAWHSNFSR ++RKNQ LERFHEFLKLE E GN+TRQE+VSMVPPLFL
Sbjct: 122 EAIKPLPWYPKNLAWHSNFSRKEIRKNQTLERFHEFLKLETEAGNMTRQESVSMVPPLFL 181

Query: 183 DVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCNLLIHQTKRM 242
           DVHP+H+VLDMCAAPGSKTFQLLEIIH+SS+PG LP G+VVAND D +R NLLIHQTKR 
Sbjct: 182 DVHPDHFVLDMCAAPGSKTFQLLEIIHESSEPGSLPNGMVVANDVDYKRSNLLIHQTKRT 241

Query: 243 CTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHN-TQLTFDRVLCDVPCSGDGTLR 302
           CT+NL+VTN+E QHFP C    N     +  S+ NPH   QL FDRVLCDVPCSGDGTLR
Sbjct: 242 CTTNLMVTNNEGQHFPSC----NTKRTLSVASETNPHPIDQLLFDRVLCDVPCSGDGTLR 301

Query: 303 KAPDIWRRWNSGVGNGLHGLQVQIGMRGASLLKVGGRMVYSTCSMNPVENEAVVAELLRK 362
           KAPDIWRRWNSG GNGLH LQV + MRG SLLKVGGRMVYSTCSMNP+E+EAVVAE+LR+
Sbjct: 302 KAPDIWRRWNSGSGNGLHSLQVVLAMRGLSLLKVGGRMVYSTCSMNPIEDEAVVAEILRR 361

Query: 363 SEGSLELVDVSNELPQLVRRPGLKKWKVRDRGVWLASYKQVSDHRQSVAIPSMFPSGRGR 422
              S+ELVDVS++LP+L+RRPGL KWKV DRG W  SYK V   ++   + SMFPSG+  
Sbjct: 362 CGCSVELVDVSDKLPELIRRPGLTKWKVHDRGGWYRSYKDVPKSQRDGVLRSMFPSGKSD 421

Query: 423 KNQADKSHNSELRGNHLDGSIGSSADVSEPTMDPVHEFDEVCEFPIERCMRIVPHDQNSG 482
           K                D S G ++     ++      +EVC+ P+E CMRI+PHDQN+G
Sbjct: 422 K----------------DSSGGKNSYEEMASISSDESAEEVCDLPLELCMRILPHDQNTG 481

Query: 483 AFFIAVLRKVAPLPAATEKPSYQNLKPVNDKSRCKPPQSITNGANVDVSASVGMDESPSE 542
            FFIAVL KV+PLP   EK                    +T   N+              
Sbjct: 482 GFFIAVLHKVSPLPDFQEK--------------------VTQKRNL-------------- 541

Query: 543 VGVDSNNQGDFNSADNKPACEFPVQDIEGTEGLDGNAADGIDKQFSEADLEVNLVDNEVS 602
                + + + NS++   + E  V D   T+  +G     ++   SE   E   +++  +
Sbjct: 542 -----STRNNINSSEKSSSYE-AVSDTVVTKPEEGTEEIVLEATVSENGFEPPEMESTWN 601

Query: 603 VNNLEITAIEDNLEESKQSTVKRKLQIQGKWKGVDPVVLFKYETVIDSIKTFYGI-NEAF 662
              +E+    D++E  ++        +QGKWKG+ PVV  + +T I+ IKTFYGI +E+F
Sbjct: 602 EGIVELAQKLDDIEGKREVP-----SMQGKWKGLYPVVFLRDQTGINGIKTFYGIKDESF 661

Query: 663 PLFGHLVTRNTDTN---HVKRIYYVSKSVKDVLEMNFSVGQQLKITSIGLKMFERQSSRE 722
           PL+GHLVTRN+D +   +VKRIYYVSK+VK+VLE+NF+VG+ LKI+S+GLKMFE+QS++E
Sbjct: 662 PLYGHLVTRNSDISSHGNVKRIYYVSKAVKNVLELNFAVGKPLKISSVGLKMFEKQSAKE 721

Query: 723 GSSAPCSFRITSEGLPVILPYITKQILTMSPMDFKHLIQHKSIKYADFADAAFGEKASNL 782
             +  CSFRITSEGLPVILPY+TKQIL  +  DFK+L+QHKSIK+ DF     GEKA+ L
Sbjct: 722 CEANCCSFRITSEGLPVILPYMTKQILYATMADFKNLLQHKSIKFLDFIHPQIGEKAAEL 766

Query: 783 MLGCCVIVLGTGGNTSSDVIKVDESTIAIGCWKGRSSLSVMVTAIDCQELLERLSNR 833
            LG CV+VL       S+  KV+ STIAIGCWKG++SL+VMVT +DC +L++RLS+R
Sbjct: 782 ALGSCVMVLVDDSQLGSEQFKVNSSTIAIGCWKGKASLTVMVTTVDCHQLIQRLSDR 766

BLAST of Cp4.1LG10g01480 vs. TAIR 10
Match: AT5G55920.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 1.9e-15
Identity = 61/200 (30.50%), Postives = 88/200 (44.00%), Query Frame = 0

Query: 169 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQ 228
           Q A S +P + L    N  ++D+ AAPG KT  +  ++  +        GL+ AN+  V 
Sbjct: 344 QGASSFLPVMALAPRENERIVDVAAAPGGKTTYIAALMKNT--------GLIYANEMKVP 403

Query: 229 RCNLLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLC 288
           R   L     RM  +N IV N++ +  P       +L              Q T DRVL 
Sbjct: 404 RLKSLTANLHRMGVTNTIVCNYDGRELP------KVLG-------------QNTVDRVLL 463

Query: 289 DVPCSGDGTLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 348
           D PCSG G + K   +              LQ Q+ +    ++    K GG +VYSTCS+
Sbjct: 464 DAPCSGTGIISKDESVKITKTMDEIKKFAHLQKQLLLAAIDMVDANSKTGGYIVYSTCSI 516

Query: 349 NPVENEAVVAELLRKSEGSL 365
              ENEAV+   L+K +  L
Sbjct: 524 MVTENEAVIDYALKKRDVKL 516

BLAST of Cp4.1LG10g01480 vs. TAIR 10
Match: AT3G13180.1 (NOL1/NOP2/sun family protein / antitermination NusB domain-containing protein )

HSP 1 Score: 78.6 bits (192), Expect = 2.7e-14
Identity = 65/201 (32.34%), Postives = 91/201 (45.27%), Query Frame = 0

Query: 172 VSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQRCN 231
           VS+V P      P   ++D CAAPG KT  +   +           G++ A D +  R  
Sbjct: 323 VSVVKP-----QPGERIMDACAAPGGKTLFMASCLKGQ--------GMIYAMDVNEGRLR 382

Query: 232 LLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLN--PHNTQLTFDRVLCD 291
           +L    K      LI T H                     SDL       ++ +D+VL D
Sbjct: 383 ILGETAKSHQVDGLITTIH---------------------SDLRVFAETNEVQYDKVLLD 442

Query: 292 VPCSGDGTLRKAPDIWRRWNSGVGNGLH--GLQVQIGMRGASLLKVGGRMVYSTCSMNPV 351
            PCSG G L K  D+  RWN  + + L    LQ ++    + L+K GG +VYSTCS++P 
Sbjct: 443 APCSGLGVLSKRADL--RWNRKLEDMLELTKLQDELLDSASKLVKHGGVLVYSTCSIDPE 487

Query: 352 ENEA-VVAELLRKSEGSLELV 368
           ENE  V A LLR  E +++ V
Sbjct: 503 ENEGRVEAFLLRHPEFTIDPV 487

BLAST of Cp4.1LG10g01480 vs. TAIR 10
Match: AT4G26600.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 77.8 bits (190), Expect = 4.6e-14
Identity = 61/200 (30.50%), Postives = 88/200 (44.00%), Query Frame = 0

Query: 169 QEAVSMVPPLFLDVHPNHYVLDMCAAPGSKTFQLLEIIHQSSKPGDLPGGLVVANDSDVQ 228
           Q A S +P + L       V+DM AAPG KT  +  ++  +        G++ AN+  V 
Sbjct: 327 QSASSFLPVMALAPREKERVVDMAAAPGGKTTYVAALMKNT--------GIIYANEMKVP 386

Query: 229 RCNLLIHQTKRMCTSNLIVTNHEAQHFPGCRAHTNILNASAAGSDLNPHNTQLTFDRVLC 288
           R   L     RM  +N IV N++       R  T +L  S+              DRVL 
Sbjct: 387 RLKSLSANLHRMGVTNTIVCNYDG------RELTKVLGQSSV-------------DRVLL 446

Query: 289 DVPCSGDGTLRKAPDIWRRWNSGVGNGLHGLQVQIGMRGASLL----KVGGRMVYSTCSM 348
           D PCSG G + K   +    ++        LQ Q+ +    L+    K GG +VYSTCS+
Sbjct: 447 DAPCSGTGVISKDESVKTSKSADDIKKFAHLQKQLILGAIDLVDANSKTGGYIVYSTCSV 499

Query: 349 NPVENEAVVAELLRKSEGSL 365
              ENEAV+   L+  +  L
Sbjct: 507 MIPENEAVIDYALKNRDVKL 499