Cp4.1LG09g11530 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g11530
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Peptidyl-tRNA hydrolase family protein
Location	Cp4.1LG09: 9769724 .. 9772854 (+)
RNA-Seq Expression	Cp4.1LG09g11530
Synteny	Cp4.1LG09g11530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGTGAAGGCTCAGGATTGAATTCATTTAAGTATTTATGTGAGAAATTAAATTCTGTTTCACTGAAAACTATTATGCCTTATTTTCTATTGGGTTAACTGGGTATGCCGTTTATCGTGTGGAACCCCCTTAGGATAGGTTTGTGGTTATCTCGAGGAAATTATGATTTATGTTGATTTACAAAGCTGTGGAATGATATTTTATTTATTGCTGTTTTTGTTATTAGCCATATCGAGTGTTGTCTGGATGCCCTTTATTAAGACGAATTGAAGGGATGGTGGATGGTGGCATATCGAGTGTTACAAAGCACTACATTTGAAAATTAATTCAGTTATTAGCCATATCAATTGTGGCTGATGCTATGATTAATTTTCCAGGTAGGTTTTGAGATGGTGGATGCTGTTGCTAAGATTGAAGGGATATCCATGAGTAGTGCTTCCTTCAAAGCTCTGATCGGGAAAGGTGTGCAGATTTTAACAATTCTTGATGAAATTAAAATATCTTGTTAGAACTTAGAAGTACATCTAGCTGCTACTTTTCTTGCCTTGAACGGACTTGCTAATAGATTACTGACTGCTTTCTTCCCTGTTTGAAGATGTGTAAACTTTTCTTCCTTATCCCTGTCATGAGATGGTTTGGCCATGAACTACAACTCGGTAAAATAACAATGGGTGCTATTGTTCCTACTATAAAATTCCATTGAAGCAAGTCCTTGTGGTAAATATTTTATTCCCCATGTAAGATTATCAGTTTTCTTGACAAGAGAGGAGGTTGGGCTGTGGGGTGGGTATCTATGTTATTCACTTTCTTTTTCCCCTACATATAAGCCTTGAATTGGGGTCCATCCTTCAACAGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGTAATTGTCTTTTATGGATTATAATTCAGCTTAACGCTTCATTTTGGGTGTCTCATTCTTCTGAGTNATTGAAGGGATATCCATGAGTAGTGCTTCCTTCAAAGCTCTGATCGGGAAAGGTGTGCAGATTTTAACAATTCTTGATGAAATTAAAATATCTTGTTAGAACTTAGAAGTACATCTAGCTGCTACTTTTCTTGCCTTGAACGGACTTGCTAATAGATTACTGACTGCTTTCTTCCCTGTTTGAAGATGTGTAAACTTTTCTTCCTTATCCCTGTCATGAGATGGTTTGGCCATGAACTACAACTCGGTAAAATAACAATGGGTGCTATTGTTCCTACTATAAAATTCCATTGAAGCAAGTCCTTGTGGTAAATATTTTATTCCCCATGTAAGATTATCAGTTTTCTTGACAAGAGAGGAGGTTGGGCTGTGGGGTGGGTATCTATGTTATTCACTTTCTTTTTCCCCTACATATAAGCCTTGAATTGGGGTCCATCCTTCAACAGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGTAATTGTCTTTTATGGATTATAATTCAGCTTAACGCTTCATTTTGGGTGTCTCATTCTTCTGAGTTGTGGCATTCATTTTCTAATATCCTTAATACTAGGAGGTATAGTGGTAGGTTTTCATATGTTTTCTTCAGGGTCATGCTGCATAATATCCCCGGTCTTTTTATTTTTATCAACAGAAGTTGCATAAAGTGAGCTTCTTAAAAAGAAAAGAACAACAAACACAAAAGTGTGATGGAGTGAGGGACATTTGATTTACTACATGATGAAAGGTTTATGTGGGCGTTTTGGCCTATCTACTTCTAGCCTTTGTATCCGAACAATGCCATTTTTTATTTACTTATTTTCTTTATTCTGTTAGGCAACCCTGCACGTGTTTGTTGTCCATTAGAAGAAAGAGAAGCATATTCCATTTTAAAGGATTTCTGGTTGAAAGATTGTGATTTTGCAGAATTTTATCCCTCAAGGAAAGGTATACTTTCTCTCTCTAAGTATGATCGTTTACTTGAAAAGTTTTGTCCGCAAAAGTTTTCCTCCCTGTAGAAAAGCATGCTTTCTTCACATATGAATATAGATTTTTACTCGAGTTGTTTAGCTTTTTAAGAGGAAAAATTTTCAGTTGACCAAGTAATAGTAAATGTAAAAGTTGATTAAAGATAACATGTGATCAAATAATTTAAGCTTAGTACAAGCATCTGGGAATACGAGTTTTTATTGAATGATTAAGATGTAATAGAATAAACATTAAGAGGAGCAAAAGACATAGAAGTGCAATTTTTAATTTGTTTATTTGGTGTATGTGTGGCAGGATGAGGAGTATTATTGATCACTTGAAAGGCAGTCGTGATTTCCCTCGGTTAAGAATAGGTACGTCCCCTGAGGCTCTGTTAACACCTCCACAGTGCTTCTACATAATATCAGCTGTTAAATGAATGAAAACATTCCAGGCATTGGGCGTCCTCCACAGCTTATGGGGGAGTAAGACAAGACAATCAGTGTAATTTTTGAACCCCATCAATGTAAGCACATTGTCTTTCTTATTGAAGCATCTCTCTCTGTCTCTTGGAATTGAAATATGTTAAGACATAAAGTAGTGATCTTTGAAGAGATCAATGAAGCCAATAACCTCCCCCACCATGCAGCAAAGAGACCGATAAAAGTTGAGAAGGACAGGAATTGATAGAACGGAGCAAGCTGCCTCTACGGCTCTACCTAACTTTGAATGCTTTAATTATCTCATCTTGACATCACCGAGGGAGGCTATTCTTTGCCTGTCTTTGTCATCTTCTTCTCCTTTATGCATTTTCTCTGTAGTTTAACCATTCGCGTCCTCTATTCTTCATGTTTGTCTTGCCTGTGTTTTCAGGTTTCTGGTGAATGCCTTCCCTATGGAATTGCCTCATACGTGGATGATTTCATGCAGGTGGATCCCACATACTTTTGAATGTACGTCATATGCTTCAAAAACTAATTATTTCATGGTATTTTTGCTGATTTTTTATTTTTATTTTTTATTATTTAATTTTGAAATTGATTCCTACTATTTTAGAAGTTTTTATTTAGTATTTATGGTTAAAGGTCGGGTTAGACCATATTGGCTTGAAGATTTAA

mRNA sequence

ATGAAGCCATATCGAGTGTTGTCTGGATGCCCTTTATTAAGACGAATTGAAGGGATGGTGGATGGTGGCATATCGAGTTTATTAGCCATATCAATTGTGGCTGATGCTATGATTAATTTTCCAGGTTTTGAGATGGTGGATGCTGTTGCTAAGATTGAAGGGATATCCATGAGTAGTGCTTCCTTCAAAGCTCTGATCGGGAAAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGCCTTGAATTGGGGTCCATCCTTCAACAGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGCAACCCTGCACGATGAGGAGTATTATTGATCACTTGAAAGGCAGTCGTGATTTCCCTCGGTTAAGAATAGGTACGTCCCCTGAGGCTCTGTTAACACCTCCACAGTGCTTCTACATAATATCAGCTGTTTCTGGTGAATGCCTTCCCTATGGAATTGCCTCATACGTGGATGATTTCATGCAGGTCGGGTTAGACCATATTGGCTTGAAGATTTAA

Coding sequence (CDS)

ATGAAGCCATATCGAGTGTTGTCTGGATGCCCTTTATTAAGACGAATTGAAGGGATGGTGGATGGTGGCATATCGAGTTTATTAGCCATATCAATTGTGGCTGATGCTATGATTAATTTTCCAGGTTTTGAGATGGTGGATGCTGTTGCTAAGATTGAAGGGATATCCATGAGTAGTGCTTCCTTCAAAGCTCTGATCGGGAAAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGCCTTGAATTGGGGTCCATCCTTCAACAGATATTCGATGACTTAGATTTGCCTTTTGCCAAATTGCGGATGCTGCCAAAGGGTGGCCATGGAGGACATAATGGGCAACCCTGCACGATGAGGAGTATTATTGATCACTTGAAAGGCAGTCGTGATTTCCCTCGGTTAAGAATAGGTACGTCCCCTGAGGCTCTGTTAACACCTCCACAGTGCTTCTACATAATATCAGCTGTTTCTGGTGAATGCCTTCCCTATGGAATTGCCTCATACGTGGATGATTTCATGCAGGTCGGGTTAGACCATATTGGCTTGAAGATTTAA

Protein sequence

MKPYRVLSGCPLLRRIEGMVDGGISSLLAISIVADAMINFPGFEMVDAVAKIEGISMSSASFKALIGKDLPFAKLRMLPKGGHGGHNGLELGSILQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIGTSPEALLTPPQCFYIISAVSGECLPYGIASYVDDFMQVGLDHIGLKI

Homology

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BLAST of Cp4.1LG09g11530 vs. ExPASy Swiss-Prot
Match: Q8GW64 (Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18440 PE=2 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 9.4e-24
Identity = 65/116 (56.03%), Postives = 76/116 (65.52%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGKDLPFAKLRMLPK-GGHGGHNGLELGSILQ---- 101
           GFEMVDA+A  EGISM++ +FKAL GK +      ML K       +G  +G I+     
Sbjct: 115 GFEMVDALADAEGISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQIVSFYKI 174

Query: 102 ------QIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
                  ++DDLDLPF KLR+LPKGGHGGHNG    MRSIID LKGSRDFPRLRIG
Sbjct: 175 PLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNG----MRSIIDRLKGSRDFPRLRIG 226

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy Swiss-Prot
Match: Q5N9Q7 (Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0693900 PE=2 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 3.6e-23
Identity = 62/116 (53.45%), Postives = 77/116 (66.38%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLPFAKLRMLPKGGHGGHNGLELGSI--- 101
           GFEM+D +A+ EGIS+SS  FKA++GK    D P    +        G +  +L S    
Sbjct: 76  GFEMIDVIAEAEGISLSSMQFKAMVGKGRIGDAPIMLAKPQTFMNASGESVGQLVSYFKI 135

Query: 102 -LQQI---FDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
            L Q+   +DDLDLPFAKLR+LPKGGHGGHNG    +RSII+HLK +RDFPRLRIG
Sbjct: 136 PLNQVLVMYDDLDLPFAKLRLLPKGGHGGHNG----VRSIINHLKQNRDFPRLRIG 187

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy Swiss-Prot
Match: Q6NLS8 (Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19830 PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 6.7e-14
Identity = 52/152 (34.21%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--------FAKLRMLPKGGHGGHNGL 101
           GFEM+D  A+  GI M+  +FKA++G+    DLP        +  L     G    +  L
Sbjct: 39  GFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVILAKPQTYMNLSGESSGPLAAYYKL 98

Query: 102 ELGSILQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIGTSP 161
            L  +L  + DD+ LP   LR+  KGGHG HNG    ++S+++H +G+R+F RLRIG   
Sbjct: 99  PLNRVL-VVHDDMQLPCGVLRLQEKGGHGCHNG----LKSVMNHFRGNREFARLRIGIGK 158

Query: 162 EALLTPPQCFYI--ISAVSGECLPYGIASYVD 180
                 P+ F +   S  + E +   +A  VD
Sbjct: 159 PPGQMDPKAFLLQKFSMPARERMDKALAEGVD 185

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy Swiss-Prot
Match: Q9M5P4 (Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea mays OX=4577 GN=CRS2 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 2.6e-13
Identity = 49/159 (30.82%), Postives = 81/159 (50.94%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIG-KDLPFAKLRMLPKGGHGGHNGLELGSI------ 101
           GFEMVD +A  EGI+M++   K+L+G   +    + ++    +  ++G  +G +      
Sbjct: 82  GFEMVDRIAAEEGITMNTIQSKSLLGIGSIGEVPVLVVKPQSYMNYSGEAIGPLAAYYQV 141

Query: 102 ----LQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIGTSPE 161
               +  I+DD  LP   LR+  KGGHG HNG    ++++I+HL G R+FPRL IG    
Sbjct: 142 PLRHILLIYDDTSLPNGVLRLQKKGGHGRHNG----LQNVIEHLDGRREFPRLSIGIGSP 201

Query: 162 ALLTPPQCFYIISAVSGECLPYGIASYVDDFMQVGLDHI 190
                P+ F +    S E +       +D  ++ G+D +
Sbjct: 202 PGKMDPRAFLLQKFSSEERV------QIDTALEQGVDAV 230

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy Swiss-Prot
Match: Q9LF14 (Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CRS2B PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 9.7e-13
Identity = 46/117 (39.32%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIG----KDLP--------FAKLRMLPKGGHGGHNGL 101
           GFEM+D +A+ EG+ M++   KALIG    +D+P        +        G    H  +
Sbjct: 66  GFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAKPQTYMNFSGESVGSLASHYRV 125

Query: 102 ELGSILQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
            L  IL  I+D++ LP   LR+ PKGG G HNG    ++S++ HL G R+FPRL IG
Sbjct: 126 PLRHIL-MIYDEMALPNGVLRLQPKGGQGYHNG----VKSVMGHLDGRRNFPRLSIG 177

[Close]

BLAST of Cp4.1LG09g11530 vs. NCBI nr
Match: XP_022994510.1 (peptidyl-tRNA hydrolase, chloroplastic isoform X4 [Cucurbita maxima])

HSP 1 Score: 129 bits (325), Expect = 3.99e-34
Identity = 87/170 (51.18%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEM+DAVA++EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 10  GFEMLDAVAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 69

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 161
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 70  SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 129

Query: 162 GTSPEALLTPPQCFYIISAVSGECLPYGIASY--VDDFMQVGLDHIGLKI 193
           G        PP     + A +    P+       +D  MQVG+D I + +
Sbjct: 130 GIG-----RPPGK---MDAANFVMRPFNKQEREELDFTMQVGVDAIRILL 162

[Close]

BLAST of Cp4.1LG09g11530 vs. NCBI nr
Match: XP_022994508.1 (peptidyl-tRNA hydrolase, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 129 bits (325), Expect = 1.33e-33
Identity = 87/170 (51.18%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEM+DAVA++EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 54  GFEMLDAVAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 113

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 161
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 114 SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 173

Query: 162 GTSPEALLTPPQCFYIISAVSGECLPYGIASY--VDDFMQVGLDHIGLKI 193
           G        PP     + A +    P+       +D  MQVG+D I + +
Sbjct: 174 GIG-----RPPGK---MDAANFVMRPFNKQEREELDFTMQVGVDAIRILL 206

[Close]

BLAST of Cp4.1LG09g11530 vs. NCBI nr
Match: KAG6572934.1 (Peptidyl-tRNA hydrolase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 130 bits (328), Expect = 1.50e-33
Identity = 88/170 (51.76%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEMVDAVA++EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 100 GFEMVDAVAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 159

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 161
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 160 SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 219

Query: 162 GTSPEALLTPPQCFYIISAVSGECLPYGIASY--VDDFMQVGLDHIGLKI 193
           G        PP     + A +    P+       +D  MQVG+D I + +
Sbjct: 220 GIG-----RPPGK---MDAANFVMRPFNKQEREELDFTMQVGVDAIRILL 252

[Close]

BLAST of Cp4.1LG09g11530 vs. NCBI nr
Match: XP_022954859.1 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X2 [Cucurbita moschata] >XP_022954860.1 peptidyl-tRNA hydrolase, chloroplastic-like isoform X2 [Cucurbita moschata] >XP_022954861.1 peptidyl-tRNA hydrolase, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 129 bits (324), Expect = 1.97e-33
Identity = 77/121 (63.64%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEMVDAVA +EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 100 GFEMVDAVAVVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 159

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 146
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 160 SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 211

[Close]

BLAST of Cp4.1LG09g11530 vs. NCBI nr
Match: ONI09114.1 (hypothetical protein PRUPE_5G218900 [Prunus persica])

HSP 1 Score: 127 bits (318), Expect = 3.80e-33
Identity = 73/123 (59.35%), Postives = 84/123 (68.29%), Query Frame = 0


Query: 40  FPGFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGS 99
           F GFEMVDA+A+ EGIS+SS SFKAL+GK    D+P  FAK +          +G  +GS
Sbjct: 2   FVGFEMVDAIAEAEGISLSSVSFKALVGKGLIGDVPVIFAKPQTFMN-----KSGESVGS 61

Query: 100 ILQQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRL 146
           I+            IFDDLDLPFAKLR+LPKGGHGGHNG    MRS+IDH KGSRDFPRL
Sbjct: 62  IVSYYKIPLKQVLVIFDDLDLPFAKLRLLPKGGHGGHNG----MRSVIDHFKGSRDFPRL 115

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy TrEMBL
Match: A0A6J1K1D8 (peptidyl-tRNA hydrolase, chloroplastic isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=4 SV=1)

HSP 1 Score: 129 bits (325), Expect = 1.93e-34
Identity = 87/170 (51.18%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEM+DAVA++EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 10  GFEMLDAVAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 69

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 161
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 70  SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 129

Query: 162 GTSPEALLTPPQCFYIISAVSGECLPYGIASY--VDDFMQVGLDHIGLKI 193
           G        PP     + A +    P+       +D  MQVG+D I + +
Sbjct: 130 GIG-----RPPGK---MDAANFVMRPFNKQEREELDFTMQVGVDAIRILL 162

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy TrEMBL
Match: A0A6J1K5D1 (peptidyl-tRNA hydrolase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=3 SV=1)

HSP 1 Score: 129 bits (325), Expect = 6.42e-34
Identity = 87/170 (51.18%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEM+DAVA++EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 54  GFEMLDAVAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 113

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 161
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 114 SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 173

Query: 162 GTSPEALLTPPQCFYIISAVSGECLPYGIASY--VDDFMQVGLDHIGLKI 193
           G        PP     + A +    P+       +D  MQVG+D I + +
Sbjct: 174 GIG-----RPPGK---MDAANFVMRPFNKQEREELDFTMQVGVDAIRILL 206

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy TrEMBL
Match: A0A6J1GTK7 (peptidyl-tRNA hydrolase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456993 PE=3 SV=1)

HSP 1 Score: 129 bits (324), Expect = 9.55e-34
Identity = 77/121 (63.64%), Postives = 84/121 (69.42%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEMVDAVA +EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 100 GFEMVDAVAVVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 159

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 146
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 160 SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 211

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy TrEMBL
Match: A0A251PC14 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G218900 PE=4 SV=1)

HSP 1 Score: 127 bits (318), Expect = 1.84e-33
Identity = 73/123 (59.35%), Postives = 84/123 (68.29%), Query Frame = 0


Query: 40  FPGFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGS 99
           F GFEMVDA+A+ EGIS+SS SFKAL+GK    D+P  FAK +          +G  +GS
Sbjct: 2   FVGFEMVDAIAEAEGISLSSVSFKALVGKGLIGDVPVIFAKPQTFMN-----KSGESVGS 61

Query: 100 ILQQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRL 146
           I+            IFDDLDLPFAKLR+LPKGGHGGHNG    MRS+IDH KGSRDFPRL
Sbjct: 62  IVSYYKIPLKQVLVIFDDLDLPFAKLRLLPKGGHGGHNG----MRSVIDHFKGSRDFPRL 115

[Close]

BLAST of Cp4.1LG09g11530 vs. ExPASy TrEMBL
Match: A0A6J1JW15 (peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490211 PE=3 SV=1)

HSP 1 Score: 129 bits (325), Expect = 2.02e-33
Identity = 87/170 (51.18%), Postives = 103/170 (60.59%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--FAKLRMLPKGGHGGHNGLELGSIL 101
           GFEM+DAVA++EGISMSSASFKALIGK    D+P   AK +          +G  +G+I+
Sbjct: 100 GFEMLDAVAEVEGISMSSASFKALIGKGFIGDVPVMLAKPQTFMNA-----SGESVGAIV 159

Query: 102 QQ----------IFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRI 161
                       IFDDLDLPFAKLRMLPKGGHGGHNG    MRSIIDHLKGSRDFPRLRI
Sbjct: 160 SYYKIPLKQVLVIFDDLDLPFAKLRMLPKGGHGGHNG----MRSIIDHLKGSRDFPRLRI 219

Query: 162 GTSPEALLTPPQCFYIISAVSGECLPYGIASY--VDDFMQVGLDHIGLKI 193
           G        PP     + A +    P+       +D  MQVG+D I + +
Sbjct: 220 GIG-----RPPGK---MDAANFVMRPFNKQEREELDFTMQVGVDAIRILL 252

[Close]

BLAST of Cp4.1LG09g11530 vs. TAIR 10
Match: AT1G18440.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 111.7 bits (278), Expect = 6.7e-25
Identity = 65/116 (56.03%), Postives = 76/116 (65.52%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGKDLPFAKLRMLPK-GGHGGHNGLELGSILQ---- 101
           GFEMVDA+A  EGISM++ +FKAL GK +      ML K       +G  +G I+     
Sbjct: 115 GFEMVDALADAEGISMNTVNFKALFGKGVIGNIPIMLAKPQTFMNLSGESVGQIVSFYKI 174

Query: 102 ------QIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
                  ++DDLDLPF KLR+LPKGGHGGHNG    MRSIID LKGSRDFPRLRIG
Sbjct: 175 PLKQVLVVYDDLDLPFGKLRLLPKGGHGGHNG----MRSIIDRLKGSRDFPRLRIG 226

[Close]

BLAST of Cp4.1LG09g11530 vs. TAIR 10
Match: AT5G19830.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 79.0 bits (193), Expect = 4.8e-15
Identity = 52/152 (34.21%), Postives = 78/152 (51.32%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIGK----DLP--------FAKLRMLPKGGHGGHNGL 101
           GFEM+D  A+  GI M+  +FKA++G+    DLP        +  L     G    +  L
Sbjct: 39  GFEMIDVFAESVGIQMNLVNFKAIMGQGFVADLPVILAKPQTYMNLSGESSGPLAAYYKL 98

Query: 102 ELGSILQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIGTSP 161
            L  +L  + DD+ LP   LR+  KGGHG HNG    ++S+++H +G+R+F RLRIG   
Sbjct: 99  PLNRVL-VVHDDMQLPCGVLRLQEKGGHGCHNG----LKSVMNHFRGNREFARLRIGIGK 158

Query: 162 EALLTPPQCFYI--ISAVSGECLPYGIASYVD 180
                 P+ F +   S  + E +   +A  VD
Sbjct: 159 PPGQMDPKAFLLQKFSMPARERMDKALAEGVD 185

[Close]

BLAST of Cp4.1LG09g11530 vs. TAIR 10
Match: AT5G16140.1 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.9e-14
Identity = 46/117 (39.32%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIG----KDLP--------FAKLRMLPKGGHGGHNGL 101
           GFEM+D +A+ EG+ M++   KALIG    +D+P        +        G    H  +
Sbjct: 66  GFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAKPQTYMNFSGESVGSLASHYRV 125

Query: 102 ELGSILQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
            L  IL  I+D++ LP   LR+ PKGG G HNG    ++S++ HL G R+FPRL IG
Sbjct: 126 PLRHIL-MIYDEMALPNGVLRLQPKGGQGYHNG----VKSVMGHLDGRRNFPRLSIG 177

[Close]

BLAST of Cp4.1LG09g11530 vs. TAIR 10
Match: AT5G16140.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 75.1 bits (183), Expect = 6.9e-14
Identity = 46/117 (39.32%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIG----KDLP--------FAKLRMLPKGGHGGHNGL 101
           GFEM+D +A+ EG+ M++   KALIG    +D+P        +        G    H  +
Sbjct: 66  GFEMIDVLARKEGVLMNTIQSKALIGIGAIEDVPILLAKPQTYMNFSGESVGSLASHYRV 125

Query: 102 ELGSILQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
            L  IL  I+D++ LP   LR+ PKGG G HNG    ++S++ HL G R+FPRL IG
Sbjct: 126 PLRHIL-MIYDEMALPNGVLRLQPKGGQGYHNG----VKSVMGHLDGRRNFPRLSIG 177

[Close]

BLAST of Cp4.1LG09g11530 vs. TAIR 10
Match: AT5G38290.2 (Peptidyl-tRNA hydrolase family protein )

HSP 1 Score: 70.5 bits (171), Expect = 1.7e-12
Identity = 39/116 (33.62%), Postives = 63/116 (54.31%), Query Frame = 0


Query: 42  GFEMVDAVAKIEGISMSSASFKALIG-KDLPFAKLRMLPKGGHGGHNGLELGSI------ 101
           GFEM+D +A+   ISM++   KAL+G   +    + ++   G+   +G  +G +      
Sbjct: 76  GFEMIDHIARATDISMNTIQSKALVGIGSVGEVPILLVKPQGYMNFSGESVGPLAAYYQI 135

Query: 102 ----LQQIFDDLDLPFAKLRMLPKGGHGGHNGQPCTMRSIIDHLKGSRDFPRLRIG 147
               +  I+DD+ L    LR+ PKGGH  HNG    ++++ +HL G R +PRL IG
Sbjct: 136 PLRHILMIYDDMGLSNGVLRLQPKGGHSQHNG----LKNVTEHLNGCRGYPRLSIG 187

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g11530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GW64	9.4e-24	56.03	Peptidyl-tRNA hydrolase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g18...	[more]
Q5N9Q7	3.6e-23	53.45	Peptidyl-tRNA hydrolase, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q6NLS8	6.7e-14	34.21	Peptidyl-tRNA hydrolase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g19...	[more]
Q9M5P4	2.6e-13	30.82	Chloroplastic group IIB intron splicing facilitator CRS2, chloroplastic OS=Zea m...	[more]
Q9LF14	9.7e-13	39.32	Chloroplastic group IIB intron splicing facilitator CRS2-B, chloroplastic OS=Ara...	[more]

BLAST of Cp4.1LG09g11530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022994510.1	3.99e-34	51.18	peptidyl-tRNA hydrolase, chloroplastic isoform X4 [Cucurbita maxima]	[more]
XP_022994508.1	1.33e-33	51.18	peptidyl-tRNA hydrolase, chloroplastic isoform X2 [Cucurbita maxima]	[more]
KAG6572934.1	1.50e-33	51.76	Peptidyl-tRNA hydrolase, chloroplastic, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022954859.1	1.97e-33	63.64	peptidyl-tRNA hydrolase, chloroplastic-like isoform X2 [Cucurbita moschata] >XP_...	[more]
ONI09114.1	3.80e-33	59.35	hypothetical protein PRUPE_5G218900 [Prunus persica]	[more]

BLAST of Cp4.1LG09g11530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K1D8	1.93e-34	51.18	peptidyl-tRNA hydrolase, chloroplastic isoform X4 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1K5D1	6.42e-34	51.18	peptidyl-tRNA hydrolase, chloroplastic isoform X2 OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1GTK7	9.55e-34	63.64	peptidyl-tRNA hydrolase, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=...	[more]
A0A251PC14	1.84e-33	59.35	Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_5G218900 PE=4 SV=1	[more]
A0A6J1JW15	2.02e-33	51.18	peptidyl-tRNA hydrolase, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN...	[more]

BLAST of Cp4.1LG09g11530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18440.1	6.7e-25	56.03	Peptidyl-tRNA hydrolase family protein	[more]
AT5G19830.1	4.8e-15	34.21	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.1	6.9e-14	39.32	Peptidyl-tRNA hydrolase family protein	[more]
AT5G16140.2	6.9e-14	39.32	Peptidyl-tRNA hydrolase family protein	[more]
AT5G38290.2	1.7e-12	33.62	Peptidyl-tRNA hydrolase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001328	Peptidyl-tRNA hydrolase	PFAM	PF01195	Pept_tRNA_hydro	coord: 98..149 e-value: 1.2E-10 score: 41.6
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 97..147
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 69..90
IPR001328	Peptidyl-tRNA hydrolase	PANTHER	PTHR17224	PEPTIDYL-TRNA HYDROLASE	coord: 42..70
IPR036416	Peptidyl-tRNA hydrolase superfamily	GENE3D	3.40.50.1470		coord: 95..168 e-value: 8.4E-13 score: 50.1
IPR036416	Peptidyl-tRNA hydrolase superfamily	SUPERFAMILY	53178	Peptidyl-tRNA hydrolase-like	coord: 98..148
None	No IPR available	PANTHER	PTHR17224:SF5	PEPTIDYL-TRNA HYDROLASE CHLOROPLASTIC	coord: 97..147
None	No IPR available	PANTHER	PTHR17224:SF5	PEPTIDYL-TRNA HYDROLASE CHLOROPLASTIC	coord: 69..90
None	No IPR available	PANTHER	PTHR17224:SF5	PEPTIDYL-TRNA HYDROLASE CHLOROPLASTIC	coord: 42..70

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g11530.1	Cp4.1LG09g11530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0004045	aminoacyl-tRNA hydrolase activity