Cp4.1LG09g11290 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g11290 vs. ExPASy Swiss-Prot
Match: Q9LMT2 (Nuclear poly(A) polymerase 1 OS=Arabidopsis thaliana OX=3702 GN=PAPS1 PE=1 SV=1)

HSP 1 Score: 879.4 bits (2271), Expect = 4.0e-254
Identity = 472/744 (63.44%), Postives = 546/744 (73.39%), Query Frame = 0

Query: 30  RNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVK 89
           + NGQR G+++PIS+ GPTE DVIKTRELEK+LQD GLYES+EEAV REEVLG LDQIVK
Sbjct: 5   QQNGQRFGVSEPISMGGPTEFDVIKTRELEKHLQDVGLYESKEEAVRREEVLGILDQIVK 64

Query: 90  IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 149
            W+K ISRAKGLN+QL+ EANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATRE DFFGE
Sbjct: 65  TWIKTISRAKGLNDQLLHEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREGDFFGE 124

Query: 150 LHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQN 209
           L +MLSEMPEV+ELHPVPDAHVP+M FK  GVSIDLLYA+L LWVIPEDLD+SQDSILQN
Sbjct: 125 LQRMLSEMPEVTELHPVPDAHVPLMGFKLNGVSIDLLYAQLPLWVIPEDLDLSQDSILQN 184

Query: 210 ADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 269
           ADEQTVRSLNGCRVTD+ILRLVPNIQ+FRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL
Sbjct: 185 ADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 244

Query: 270 VARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHL 329
           VARICQL+PNALPN+LVSRFFRVF QW WPN + LCS +EGSLGL VWDPR NPKDR H+
Sbjct: 245 VARICQLYPNALPNILVSRFFRVFYQWNWPNAIFLCSPDEGSLGLQVWDPRINPKDRLHI 304

Query: 330 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYK 389
           MPIITPAYPCMNSSYNVS STLRIM  EFQRG++ICE ME NKADWDTLFEP+ FFEAYK
Sbjct: 305 MPIITPAYPCMNSSYNVSESTLRIMKGEFQRGNEICEAMESNKADWDTLFEPFAFFEAYK 364

Query: 390 NYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC 449
           NYLQIDI+A N DDLRKWKGWVESRLRQLTLKIERH +  L CHPHP DF D S+P H  
Sbjct: 365 NYLQIDISAANVDDLRKWKGWVESRLRQLTLKIERH-FKMLHCHPHPHDFQDTSRPLHCS 424

Query: 450 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 509
           YFMGLQRKQGVPA+ GEQFD+R TVEEFKH+VN YTLW PGMEI V H+K+R++P FVFP
Sbjct: 425 YFMGLQRKQGVPAAEGEQFDIRRTVEEFKHTVNAYTLWIPGMEISVGHIKRRSLPNFVFP 484

Query: 510 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEV---------AESIDGVDDRRKRMRL 569
           GGVRP   SK TWDS R SE + S ++                 A S   VD ++++   
Sbjct: 485 GGVRPSHTSKGTWDSNRRSEHRNSSTSSAPAATTTTTEMSSESKAGSNSPVDGKKRKWGD 544

Query: 570 DDNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQ 629
            +      RN + + V  S P E  E      + + S+  + S+P        + D  + 
Sbjct: 545 SETLTDQPRNSKHIAV--SVPVENCE----GGSPNPSVGSICSSP--------MKDYCT- 604

Query: 630 NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDY 689
            NG    +  D   N+V+ + D       E L I+KI +    +H+    ELEE   FD+
Sbjct: 605 -NGKSEPISKDPPENVVAFSKD-----PPESLPIEKIATP--QAHET--EELEE--SFDF 664

Query: 690 KNQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSS 749
            NQ       +Q S  ++ ++ T+++ P     EA  + S +    +EELE      P +
Sbjct: 665 GNQ-----VIEQISHKVAVLSATATIPPF----EATSNGSPFPYEAVEELEVLPTRQPDA 709

Query: 750 TGTAPAPVAARKPIIRLSFTSLGK 765
                  V  RKPII+LSFTSLGK
Sbjct: 725 AHR--PSVQQRKPIIKLSFTSLGK 709

BLAST of Cp4.1LG09g11290 vs. ExPASy Swiss-Prot
Match: Q8VYW1 (Nuclear poly(A) polymerase 4 OS=Arabidopsis thaliana OX=3702 GN=PAPS4 PE=1 SV=1)

HSP 1 Score: 700.3 bits (1806), Expect = 3.3e-200
Identity = 358/660 (54.24%), Postives = 457/660 (69.24%), Query Frame = 0

Query: 31  NNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKI 90
           N+ +  GIT P+SL+GP+  D+ +  ELEKYL D GLYES+++ + REEVLGR+DQIVK 
Sbjct: 16  NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRIDQIVKH 75

Query: 91  WVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGEL 150
           WVK +++ +G  +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP +  REEDFF  L
Sbjct: 76  WVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFIIL 135

Query: 151 HKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNA 210
           H +L+EM EV+ELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLD+S  S+L   
Sbjct: 136 HDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCEV 195

Query: 211 DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLV 270
           DE TVRSLNGCRV D+IL+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLV
Sbjct: 196 DEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLV 255

Query: 271 ARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLM 330
           AR+CQL+PNA+P+MLVSRFFRV+TQWRWPNPVMLC+ EE  LG PVWD R+N +DRYHLM
Sbjct: 256 ARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHLM 315

Query: 331 PIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKN 390
           PIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK  W +LFE Y FFEAYKN
Sbjct: 316 PIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYKN 375

Query: 391 YLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC- 450
           YLQ+DI A +A+DL  WKGWVESR RQLTLKIER T   L CHP P ++ D ++ F HC 
Sbjct: 376 YLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHCA 435

Query: 451 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 510
           +FMGLQR +GV     +QFD+R TV+EF+  VNMY  WKPGM+++VSHV++R +P FVFP
Sbjct: 436 FFMGLQRAEGVGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLPPFVFP 495

Query: 511 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSR 570
            G R  R S+     +     K+ +   V     V E        R   R +D+   + R
Sbjct: 496 NGYRRPRQSR----HQNLPGGKSGEDGSVSHSGSVVE--------RHAKRKNDSEMMDVR 555

Query: 571 NGECLVVVHSHPEEVHEVSQVS-------TTSSCSIKDLNSTPTSANNLE----NLADVS 630
             +        P+ +  VS  +       T   C+++   S    A+NL      L D++
Sbjct: 556 PEKPEKRASLSPQSLDIVSPENSAITTGWTPPVCNLRRPPSEEIEADNLNTECTELTDLA 615

Query: 631 SQ--NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELD 677
               N+G    +EVD       + A    C +  +   + +  DS D    +  + E LD
Sbjct: 616 RNECNSGSEQVLEVD-------SMAVVQECSDPAEPLGKCVTPDSVDVVACVSGQEENLD 656

BLAST of Cp4.1LG09g11290 vs. ExPASy Swiss-Prot
Match: O82312 (Nuclear poly(A) polymerase 2 OS=Arabidopsis thaliana OX=3702 GN=PAPS2 PE=1 SV=2)

HSP 1 Score: 697.6 bits (1799), Expect = 2.2e-199
Identity = 320/484 (66.12%), Postives = 393/484 (81.20%), Query Frame = 0

Query: 37  GITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKIWVKAIS 96
           GIT+P+S++GP+  DV +  ELEK+L D GLYES+EE + REEV+ R+DQIVK WVK ++
Sbjct: 24  GITEPLSIAGPSAADVKRNLELEKFLVDEGLYESKEETMRREEVVVRIDQIVKHWVKQLT 83

Query: 97  RAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGELHKMLSE 156
           R +G  +Q+V++ANA IFTFGSYRLGVHGP ADIDTLCVGP +  REEDFF     +L+E
Sbjct: 84  RQRGYTDQMVEDANAVIFTFGSYRLGVHGPMADIDTLCVGPSYVNREEDFFIFFRDILAE 143

Query: 157 MPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNADEQTVR 216
           M EV+EL PV DAHVPVMKFKF G+SIDLLYA +SL VIP+DLD+S  S+L + DEQTVR
Sbjct: 144 MEEVTELQPVTDAHVPVMKFKFQGISIDLLYASISLLVIPQDLDISNSSVLCDVDEQTVR 203

Query: 217 SLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQL 276
           SLNGCRV D+IL+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLVAR+CQ 
Sbjct: 204 SLNGCRVADQILKLVPNSEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLVARLCQF 263

Query: 277 FPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLMPIITPA 336
           +PNA+P+MLVSRFFRV+TQWRWPNPVMLC+ EE  L  PVWDPR+N +DRYHLMPIITPA
Sbjct: 264 YPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDDLSFPVWDPRKNHRDRYHLMPIITPA 323

Query: 337 YPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKNYLQIDI 396
           YPCMNSSYNVS STLR+MTE+FQ G+ IC+ +E NK  W +LF+ Y FFEAYKNYLQ+D+
Sbjct: 324 YPCMNSSYNVSQSTLRVMTEQFQFGNTICQEIELNKQHWSSLFQQYMFFEAYKNYLQVDV 383

Query: 397 TAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC-YFMGLQ 456
            A +A+DL  WKGWVESR RQLTLKIER T   L CHP P ++ D SK F HC +FMGLQ
Sbjct: 384 LAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTSKQFRHCAFFMGLQ 443

Query: 457 RKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFPGGVRPL 516
           R  G      +QFD+R TV+EF+  VNMY  W+PGM+++VSHV++R +P+FVFP G +  
Sbjct: 444 RADGFGGQECQQFDIRGTVDEFRQEVNMYMFWRPGMDVHVSHVRRRQLPSFVFPNGYKRS 503

Query: 517 RASK 520
           R S+
Sbjct: 504 RQSR 507

BLAST of Cp4.1LG09g11290 vs. ExPASy Swiss-Prot
Match: Q54J73 (Poly(A) polymerase OS=Dictyostelium discoideum OX=44689 GN=papA PE=3 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 8.4e-127
Identity = 262/638 (41.07%), Postives = 373/638 (58.46%), Query Frame = 0

Query: 36  LGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKIWVKAI 95
           LG+T+PIS + P+  D   + ELE  L    L+ES EE+  REE+LG+L+QIV+ W K +
Sbjct: 53  LGVTEPISTAPPSSIDFKLSTELENTLISFNLFESPEESRKREEILGKLNQIVREWAKQV 112

Query: 96  SRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGELHKMLS 155
           S  KG  EQ   E  AKIFTFGSYRLGVHGPG+DIDTLCVGP+H  R  DFF +L  +L 
Sbjct: 113 SLKKGYPEQTASEVVAKIFTFGSYRLGVHGPGSDIDTLCVGPKHIMR-SDFFDDLSDILK 172

Query: 156 EMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDL-DVSQDSILQNADEQT 215
             PE++E   V DA VPV+   F+G+ IDL+YAKL+L  IPE+L D+  +S L+N DE++
Sbjct: 173 VHPEITEFTTVKDAFVPVITMVFSGIPIDLIYAKLALTAIPEELNDLIDESFLKNIDEKS 232

Query: 216 VRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARIC 275
           + SLNGCRVTD+IL+LVPNI +FR  LRC++ WA RRG+YSN+ GFLGG++WALL ARIC
Sbjct: 233 ILSLNGCRVTDQILKLVPNIPNFRMALRCIKLWAIRRGIYSNILGFLGGVSWALLTARIC 292

Query: 276 QLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGS-LGLPVWDPRRNPKDRYHLMPII 335
           QL+PN+ P+ ++ RFF+V+  W+WP P++LC  +EG  LG  VW+P+R   D+ HLMPII
Sbjct: 293 QLYPNSAPSTIIHRFFKVYEIWKWPAPILLCHIQEGGILGPKVWNPKR---DKAHLMPII 352

Query: 336 TPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKNYLQ 395
           TPAYP MNS+YNVS STL++M  EF RG +I   +E  +  W  L E   FF  Y  Y++
Sbjct: 353 TPAYPSMNSTYNVSKSTLQLMKSEFVRGAEITRKIETGECTWKNLLEKCDFFTRYSFYIE 412

Query: 396 IDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDK----SKPFHHC 455
           ID  + N +D RKW+GW+ES+LR L   +E     K    P+P  F++     + P   C
Sbjct: 413 IDCYSMNEEDSRKWEGWIESKLRFLISNLESTPKMKFAV-PYPKGFTNNLHKANNPDQIC 472

Query: 456 --YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPG---MEIYVSHVKKRNIP 515
             +FMGL           +  D+   V EF   +  +   +P    M+I V ++KK+ +P
Sbjct: 473 TSFFMGLSFNFSNTPGADKSVDLTKAVTEFTGIIKDWLRTQPNPDTMDIKVQYIKKKQLP 532

Query: 516 TFV-FPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDN 575
            FV   G   P++ +K     +RSS  + S +                   RK+++ +++
Sbjct: 533 AFVKDEGPEEPVKTTK-----KRSSTGEPSAT-------------------RKKLKSENS 592

Query: 576 AN-TNSRNGECLVVVHSHP---------EEVHEVSQVSTTSSCSIKDLNSTPTS------ 635
            N  NS        ++S P               +  +TT++ +   + STPTS      
Sbjct: 593 DNKLNSPKSPITTNINSTPTTSTPTTTANTTTNTTTATTTTTTTTVPITSTPTSNISSPT 652

Query: 636 ANNLENLADVSSQNNGDHGSMEVDHSTNIVSAAADTSS 646
            N+ E     S+     + S+    +T  +++    S+
Sbjct: 653 MNSTELTTPTSTSTTTSNDSITTPPTTTTINSVQPPSA 661

BLAST of Cp4.1LG09g11290 vs. ExPASy Swiss-Prot
Match: Q9BWT3 (Poly(A) polymerase gamma OS=Homo sapiens OX=9606 GN=PAPOLG PE=1 SV=2)

HSP 1 Score: 441.8 bits (1135), Expect = 2.1e-122
Identity = 226/511 (44.23%), Postives = 323/511 (63.21%), Query Frame = 0

Query: 27  LNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQ 86
           L+ +   +  GIT PISL+ P E D I T++L   ++  G++E +EE   R  VLG+L+ 
Sbjct: 10  LDSQRQQKHYGITSPISLASPKEIDHIYTQKLIDAMKPFGVFEDEEELNHRLVVLGKLNN 69

Query: 87  IVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDF 146
           +VK W+  +S +K L   +V     KIFTFGSYRLGVH  GADID LCV PRH  R  DF
Sbjct: 70  LVKEWISDVSESKNLPPSVVATVGGKIFTFGSYRLGVHTKGADIDALCVAPRHVER-SDF 129

Query: 147 FGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSI 206
           F    + L     +  L  V DA VPV+KF+F G+ IDL++A+L++  I ++LD+  DS 
Sbjct: 130 FQSFFEKLKHQDGIRNLRAVEDAFVPVIKFEFDGIEIDLVFARLAIQTISDNLDLRDDSR 189

Query: 207 LQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINW 266
           L++ D + +RSLNGCRVTD IL LVPN ++FR TLR ++ WAKRRG+YSN+ GFLGG++W
Sbjct: 190 LRSLDIRCIRSLNGCRVTDEILHLVPNKETFRLTLRAVKLWAKRRGIYSNMLGFLGGVSW 249

Query: 267 ALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDR 326
           A+LVAR CQL+PNA  + LV +FF VF++W WPNPV+L   EE +L LPVWDPR NP DR
Sbjct: 250 AMLVARTCQLYPNAAASTLVHKFFLVFSKWEWPNPVLLKQPEESNLNLPVWDPRVNPSDR 309

Query: 327 YHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFE 386
           YHLMPIITPAYP  NS+YNVS+ST  +M EEF++G  + + + + K+DW  L EP  FF+
Sbjct: 310 YHLMPIITPAYPQQNSTYNVSTSTRTVMVEEFKQGLAVTDEILQGKSDWSKLLEPPNFFQ 369

Query: 387 AYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDF-----SD 446
            Y++Y+ +  +A   ++  +W G VES++R L   +ER+ +  L  H +P  F       
Sbjct: 370 KYRHYIVLTASASTEENHLEWVGLVESKIRVLVGNLERNEFITL-AHVNPQSFPGNKEHH 429

Query: 447 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSV----NMYTLWKPGMEIYVSH 506
           K   +   +F+G+  ++ V  +     D+   ++ F  +V    N   + K GM+I  +H
Sbjct: 430 KDNNYVSMWFLGIIFRR-VENAESVNIDLTYDIQSFTDTVYRQANNINMLKEGMKIEATH 489

Query: 507 VKKRNIPTFVFPGGVRPLRASKVTWDSRRSS 529
           VKK+ +  ++ P  +   +  +   D  RSS
Sbjct: 490 VKKKQLHHYL-PAEILQKKKKQSLSDVNRSS 516

BLAST of Cp4.1LG09g11290 vs. NCBI nr
Match: XP_023541715.1 (nuclear poly(A) polymerase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1497 bits (3875), Expect = 0.0
Identity = 748/748 (100.00%), Postives = 748/748 (100.00%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 120

Query: 142 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 201
           REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV
Sbjct: 121 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 180

Query: 202 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 261
           SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL
Sbjct: 181 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 240

Query: 262 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 321
           GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR
Sbjct: 241 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 300

Query: 322 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 381
           NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP
Sbjct: 301 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 360

Query: 382 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 441
           YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD
Sbjct: 361 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 420

Query: 442 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 501
           KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR
Sbjct: 421 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 480

Query: 502 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 561
           NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD
Sbjct: 481 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 540

Query: 562 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 621
           DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN
Sbjct: 541 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 600

Query: 622 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 681
           NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK
Sbjct: 601 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 660

Query: 682 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 741
           NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST
Sbjct: 661 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 720

Query: 742 GTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           GTAPAPVAARKPIIRLSFTSLGKAGKSS
Sbjct: 721 GTAPAPVAARKPIIRLSFTSLGKAGKSS 748

BLAST of Cp4.1LG09g11290 vs. NCBI nr
Match: XP_022954916.1 (nuclear poly(A) polymerase 1-like [Cucurbita moschata])

HSP 1 Score: 1490 bits (3857), Expect = 0.0
Identity = 744/748 (99.47%), Postives = 745/748 (99.60%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 120

Query: 142 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 201
           REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV
Sbjct: 121 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 180

Query: 202 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 261
           SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL
Sbjct: 181 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 240

Query: 262 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 321
           GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR
Sbjct: 241 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 300

Query: 322 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 381
           NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP
Sbjct: 301 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 360

Query: 382 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 441
           YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD
Sbjct: 361 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 420

Query: 442 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 501
           KS+PFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR
Sbjct: 421 KSRPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 480

Query: 502 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 561
           NIP FVFPGGVRP RASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD
Sbjct: 481 NIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 540

Query: 562 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 621
           DNANTNSRNGECL VVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN
Sbjct: 541 DNANTNSRNGECLAVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 600

Query: 622 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 681
           NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK
Sbjct: 601 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 660

Query: 682 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 741
           NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST
Sbjct: 661 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 720

Query: 742 GTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           GTAPAPVAARKPIIRLSFTSLGKAGKSS
Sbjct: 721 GTAPAPVAARKPIIRLSFTSLGKAGKSS 748

BLAST of Cp4.1LG09g11290 vs. NCBI nr
Match: KAG6572954.1 (Nuclear poly(A) polymerase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1483 bits (3839), Expect = 0.0
Identity = 741/748 (99.06%), Postives = 741/748 (99.06%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 120

Query: 142 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 201
           REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV
Sbjct: 121 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 180

Query: 202 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 261
           SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL
Sbjct: 181 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 240

Query: 262 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 321
           GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR
Sbjct: 241 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 300

Query: 322 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 381
           NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP
Sbjct: 301 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 360

Query: 382 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 441
           YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD
Sbjct: 361 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 420

Query: 442 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 501
           KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR
Sbjct: 421 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 480

Query: 502 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 561
           NIP FVFPGGVRP RASKVTWDSRRS ELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD
Sbjct: 481 NIPNFVFPGGVRPSRASKVTWDSRRSLELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 540

Query: 562 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 621
           DNANTNSRNGECL VVHSHPEEVHEVSQVS TSSCSIKDLNSTPTSANNLENLADVSSQN
Sbjct: 541 DNANTNSRNGECLAVVHSHPEEVHEVSQVSATSSCSIKDLNSTPTSANNLENLADVSSQN 600

Query: 622 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 681
           NGDHGSMEVDHSTN VSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK
Sbjct: 601 NGDHGSMEVDHSTNSVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 660

Query: 682 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 741
           NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST
Sbjct: 661 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 720

Query: 742 GTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           GTAPAPVAARKPIIRLSFTSLGK GKSS
Sbjct: 721 GTAPAPVAARKPIIRLSFTSLGKTGKSS 748

BLAST of Cp4.1LG09g11290 vs. NCBI nr
Match: XP_022994133.1 (nuclear poly(A) polymerase 1-like [Cucurbita maxima])

HSP 1 Score: 1473 bits (3813), Expect = 0.0
Identity = 738/748 (98.66%), Postives = 741/748 (99.06%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDV KTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVRKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 120

Query: 142 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 201
           REEDFFGELHKMLSEMPEVSELHPVPDAHVPVM+FKFAGVSIDLLYAKLSLWVIPEDLDV
Sbjct: 121 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKFAGVSIDLLYAKLSLWVIPEDLDV 180

Query: 202 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 261
           SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL
Sbjct: 181 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 240

Query: 262 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 321
           GGINWALLVARICQLFPNALPNMLV RFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR
Sbjct: 241 GGINWALLVARICQLFPNALPNMLVYRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 300

Query: 322 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 381
           NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP
Sbjct: 301 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 360

Query: 382 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 441
           YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD
Sbjct: 361 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 420

Query: 442 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 501
           KS+PFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR
Sbjct: 421 KSRPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 480

Query: 502 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 561
           NIP FVFPGGVRP RASKVTWDSRRSSELKASDSAQVDRPFEVAES+DGVDDRRKRMRLD
Sbjct: 481 NIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESMDGVDDRRKRMRLD 540

Query: 562 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 621
           DN NTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN
Sbjct: 541 DNVNTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 600

Query: 622 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 681
           NGDHGSMEVDHSTNIVSAAADTSSCK AEKLAIQKILSDSYDSHQAL SELEELDDFDYK
Sbjct: 601 NGDHGSMEVDHSTNIVSAAADTSSCK-AEKLAIQKILSDSYDSHQALSSELEELDDFDYK 660

Query: 682 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 741
           NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST
Sbjct: 661 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 720

Query: 742 GTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           GTAPAPVAARKPIIRLSFTSLGKAGKSS
Sbjct: 721 GTAPAPVAARKPIIRLSFTSLGKAGKSS 747

BLAST of Cp4.1LG09g11290 vs. NCBI nr
Match: KAG7012136.1 (Nuclear poly(A) polymerase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1459 bits (3777), Expect = 0.0
Identity = 740/780 (94.87%), Postives = 740/780 (94.87%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLG------------------- 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLG                   
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGSQRFSTSIMYKPEDYTMIA 120

Query: 142 ----VHGPGADIDTLCVGPRHATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKF 201
               VHGPGADIDTLCVGPRHATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKF
Sbjct: 121 TVFEVHGPGADIDTLCVGPRHATREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKF 180

Query: 202 AGVSIDLLYAKLSLWVIPEDLDVSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFR 261
           AGVSIDLLYAKLSLWVIPEDLDVSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFR
Sbjct: 181 AGVSIDLLYAKLSLWVIPEDLDVSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFR 240

Query: 262 TTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRW 321
           TTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRW
Sbjct: 241 TTLRCMRFWAKRRGVYSNVSGFLGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRW 300

Query: 322 PNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEF 381
           PNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEF
Sbjct: 301 PNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEF 360

Query: 382 QRGHDICEVMEENKADWDTLFEPYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQL 441
           QRGHDICEVMEENKADWDTLFEPYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQL
Sbjct: 361 QRGHDICEVMEENKADWDTLFEPYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQL 420

Query: 442 TLKIE---------RHTYNKLQCHPHPGDFSDKSKPFHHCYFMGLQRKQGVPASGGEQFD 501
           TLKIE         RHTYNKLQCHPHPGDFSDKSKPFHHCYFMGLQRKQGVPASGGEQFD
Sbjct: 421 TLKIELGSIVMQIERHTYNKLQCHPHPGDFSDKSKPFHHCYFMGLQRKQGVPASGGEQFD 480

Query: 502 MRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFPGGVRPLRASKVTWDSRRSSE 561
           MRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIP FVFPGGVRP RASKVTWDSRRS E
Sbjct: 481 MRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPNFVFPGGVRPSRASKVTWDSRRSLE 540

Query: 562 LKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSRNGECLVVVHSHPEEVHEVSQ 621
           LKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSRNGECL VVHSHPEEVHEVSQ
Sbjct: 541 LKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSRNGECLAVVHSHPEEVHEVSQ 600

Query: 622 VSTTSSCSIKDLNSTPTSANNLENLADVSSQNNGDHGSMEVDHSTNIVSAAADTSSCKEA 681
           VS TSSCSIKDLNSTPTSANNLENLADVSSQNNGDHGSMEVDHSTN VSAAADTSSCKEA
Sbjct: 601 VSATSSCSIKDLNSTPTSANNLENLADVSSQNNGDHGSMEVDHSTNSVSAAADTSSCKEA 660

Query: 682 EKLAIQKILSDSYDSHQALPSELEELDDFDYKNQAKDFGATKQGSPLMSSVANTSSVVPM 741
           EKLAIQKILSDSYDSHQALPSELEELDDFDYKNQAKDFGATKQGSPLMSSVANTSSVVPM
Sbjct: 661 EKLAIQKILSDSYDSHQALPSELEELDDFDYKNQAKDFGATKQGSPLMSSVANTSSVVPM 720

Query: 742 TSCNEARQSSSSYSNGGLEELEPAELVAPSSTGTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           TSCNEARQSSSSYSNGGLEELEPAELVAPSSTGTA APVAARKPIIRLSFTSLGK GKSS
Sbjct: 721 TSCNEARQSSSSYSNGGLEELEPAELVAPSSTGTAQAPVAARKPIIRLSFTSLGKTGKSS 780

BLAST of Cp4.1LG09g11290 vs. ExPASy TrEMBL
Match: A0A6J1GS98 (Polynucleotide adenylyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111457031 PE=3 SV=1)

HSP 1 Score: 1490 bits (3857), Expect = 0.0
Identity = 744/748 (99.47%), Postives = 745/748 (99.60%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 120

Query: 142 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 201
           REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV
Sbjct: 121 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 180

Query: 202 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 261
           SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL
Sbjct: 181 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 240

Query: 262 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 321
           GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR
Sbjct: 241 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 300

Query: 322 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 381
           NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP
Sbjct: 301 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 360

Query: 382 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 441
           YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD
Sbjct: 361 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 420

Query: 442 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 501
           KS+PFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR
Sbjct: 421 KSRPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 480

Query: 502 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 561
           NIP FVFPGGVRP RASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD
Sbjct: 481 NIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 540

Query: 562 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 621
           DNANTNSRNGECL VVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN
Sbjct: 541 DNANTNSRNGECLAVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 600

Query: 622 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 681
           NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK
Sbjct: 601 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 660

Query: 682 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 741
           NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST
Sbjct: 661 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 720

Query: 742 GTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           GTAPAPVAARKPIIRLSFTSLGKAGKSS
Sbjct: 721 GTAPAPVAARKPIIRLSFTSLGKAGKSS 748

BLAST of Cp4.1LG09g11290 vs. ExPASy TrEMBL
Match: A0A6J1K4A6 (Polynucleotide adenylyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111489961 PE=3 SV=1)

HSP 1 Score: 1473 bits (3813), Expect = 0.0
Identity = 738/748 (98.66%), Postives = 741/748 (99.06%), Query Frame = 0

Query: 22  MGSPALNGRNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVL 81
           MGSPALNGRNNGQRLGITDPISLSGPTEDDV KTRELEKYLQDAGLYESQEEAVSREEVL
Sbjct: 1   MGSPALNGRNNGQRLGITDPISLSGPTEDDVRKTRELEKYLQDAGLYESQEEAVSREEVL 60

Query: 82  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 141
           GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT
Sbjct: 61  GRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHAT 120

Query: 142 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDV 201
           REEDFFGELHKMLSEMPEVSELHPVPDAHVPVM+FKFAGVSIDLLYAKLSLWVIPEDLDV
Sbjct: 121 REEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKFAGVSIDLLYAKLSLWVIPEDLDV 180

Query: 202 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 261
           SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL
Sbjct: 181 SQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFL 240

Query: 262 GGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 321
           GGINWALLVARICQLFPNALPNMLV RFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR
Sbjct: 241 GGINWALLVARICQLFPNALPNMLVYRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRR 300

Query: 322 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 381
           NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP
Sbjct: 301 NPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEP 360

Query: 382 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 441
           YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD
Sbjct: 361 YPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSD 420

Query: 442 KSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 501
           KS+PFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR
Sbjct: 421 KSRPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKR 480

Query: 502 NIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLD 561
           NIP FVFPGGVRP RASKVTWDSRRSSELKASDSAQVDRPFEVAES+DGVDDRRKRMRLD
Sbjct: 481 NIPNFVFPGGVRPSRASKVTWDSRRSSELKASDSAQVDRPFEVAESMDGVDDRRKRMRLD 540

Query: 562 DNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 621
           DN NTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN
Sbjct: 541 DNVNTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQN 600

Query: 622 NGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDYK 681
           NGDHGSMEVDHSTNIVSAAADTSSCK AEKLAIQKILSDSYDSHQAL SELEELDDFDYK
Sbjct: 601 NGDHGSMEVDHSTNIVSAAADTSSCK-AEKLAIQKILSDSYDSHQALSSELEELDDFDYK 660

Query: 682 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 741
           NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST
Sbjct: 661 NQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSST 720

Query: 742 GTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           GTAPAPVAARKPIIRLSFTSLGKAGKSS
Sbjct: 721 GTAPAPVAARKPIIRLSFTSLGKAGKSS 747

BLAST of Cp4.1LG09g11290 vs. ExPASy TrEMBL
Match: A0A0A0LQF9 (Polynucleotide adenylyltransferase OS=Cucumis sativus OX=3659 GN=Csa_1G042640 PE=3 SV=1)

HSP 1 Score: 1334 bits (3453), Expect = 0.0
Identity = 673/751 (89.61%), Postives = 704/751 (93.74%), Query Frame = 0

Query: 22  MGSPALNGRNNGQ-RLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEV 81
           MGSPAL GRNNGQ RLGITDPISLSGPTE DV+KTRELEKYLQDAGLYESQE+AV+REEV
Sbjct: 1   MGSPALCGRNNGQQRLGITDPISLSGPTEYDVLKTRELEKYLQDAGLYESQEDAVNREEV 60

Query: 82  LGRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 141
           LGRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61  LGRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120

Query: 142 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 201
           TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVM+FK +GVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKLSGVSIDLLYAKLSLWVIPEDLD 180

Query: 202 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 261
           +SQDSILQN DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 ISQDSILQNTDEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240

Query: 262 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 321
           LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGL VWDPR
Sbjct: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLQVWDPR 300

Query: 322 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 381
           RNPKDRYHLMPIITPAYPCMNSSYNVS+STLRIMTEEF+RGHDICEVMEENK+DWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSASTLRIMTEEFRRGHDICEVMEENKSDWDTLFE 360

Query: 382 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 441
           PYPFFEAYKNYLQIDITAEN DD+R WKGWVESRLRQLTLKIERHTYN LQCHP+PGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENDDDIRIWKGWVESRLRQLTLKIERHTYNMLQCHPYPGDFS 420

Query: 442 DKSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKK 501
           DKS+PFHHCYFMGLQRKQG PASGGEQFD+RLTV+EFKHSVN+YT  K GMEIYVSHVK+
Sbjct: 421 DKSRPFHHCYFMGLQRKQGGPASGGEQFDIRLTVDEFKHSVNVYTQRKRGMEIYVSHVKR 480

Query: 502 RNIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRL 561
           R+IP FVFPGGVRP RASK+TWD RRSSELKASDS QVD P E  ES+DG DDRRKR+R+
Sbjct: 481 RSIPNFVFPGGVRPSRASKLTWDIRRSSELKASDSTQVDSPSEATESLDG-DDRRKRIRI 540

Query: 562 DDNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQ 621
           DDNANTN RNGECL + HSHPEEVHEVSQVS TSSCSIKD+N  PTSANNLENLADVSSQ
Sbjct: 541 DDNANTNLRNGECLAMAHSHPEEVHEVSQVSNTSSCSIKDVNFIPTSANNLENLADVSSQ 600

Query: 622 NNGDHGSMEVDHSTNIVS-AAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFD 681
           NNGDHGS+ V  STN VS AAADTS+CKEAEKLAIQKILSDSYDSHQ  P E EEL+DFD
Sbjct: 601 NNGDHGSLRVSPSTNNVSDAAADTSNCKEAEKLAIQKILSDSYDSHQDFPCETEELEDFD 660

Query: 682 YKNQAKDFGATKQGSPLMSSVANTSSVV-PMTSCNEARQSSSSYSNGGLEELEPAELVAP 741
           Y NQAKDFGATKQGSP+MSSVANTS +V P  SCNEARQSSSSY NGGLEELEPAE+VAP
Sbjct: 661 YNNQAKDFGATKQGSPMMSSVANTSPLVLPTVSCNEARQSSSSYYNGGLEELEPAEIVAP 720

Query: 742 SSTGTAPAPVAARKPIIRLSFTSLGKAGKSS 769
            STGTAP  VA RKPIIRLSFTSLGKAGKSS
Sbjct: 721 LSTGTAP--VAERKPIIRLSFTSLGKAGKSS 748

BLAST of Cp4.1LG09g11290 vs. ExPASy TrEMBL
Match: A0A5D3BLG8 (Polynucleotide adenylyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002220 PE=3 SV=1)

HSP 1 Score: 1324 bits (3426), Expect = 0.0
Identity = 667/750 (88.93%), Postives = 699/750 (93.20%), Query Frame = 0

Query: 22  MGSPALNGRNNGQ-RLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEV 81
           MGSPAL GRNNGQ RLGITDPISLSGPTE DV+KTRELEKYLQDAGLYESQEEAV+REEV
Sbjct: 1   MGSPALCGRNNGQQRLGITDPISLSGPTEYDVLKTRELEKYLQDAGLYESQEEAVNREEV 60

Query: 82  LGRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 141
           LGRLDQIVKIWVKAISR+KGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61  LGRLDQIVKIWVKAISRSKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120

Query: 142 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 201
           TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVM+FK +GVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKLSGVSIDLLYAKLSLWVIPEDLD 180

Query: 202 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 261
           +SQ+SILQN DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 ISQESILQNTDEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240

Query: 262 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 321
           LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGLPVWDPR
Sbjct: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLPVWDPR 300

Query: 322 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 381
           RNPKDRYHLMPIITPAYPCMNSSYNVS+STLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSASTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360

Query: 382 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 441
           PYPFFEAYKNYLQIDITAEN DD+R WKGWVESRLRQLTLKIERHTYN LQCHP+PGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENDDDIRIWKGWVESRLRQLTLKIERHTYNMLQCHPYPGDFS 420

Query: 442 DKSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKK 501
           DKS+PFHHCYFMGLQRKQG PASG EQFD+RLTV+EFK SVN+YT  K GMEIYVSHVK+
Sbjct: 421 DKSRPFHHCYFMGLQRKQGGPASGSEQFDIRLTVDEFKRSVNVYTQRKRGMEIYVSHVKR 480

Query: 502 RNIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRL 561
           R+IP FVFPGGVRP RASK+TWD RRSSELKASDS QVD P EV ES+DG DDRRK++R+
Sbjct: 481 RSIPNFVFPGGVRPSRASKLTWDIRRSSELKASDSTQVDSPSEVTESLDG-DDRRKKIRI 540

Query: 562 DDNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQ 621
           DD+ANTN RNGECL   +SH EEVHEVSQVS TSSCSIKD+N  PTS NNLENLADVSSQ
Sbjct: 541 DDDANTNLRNGECLAAANSHHEEVHEVSQVSNTSSCSIKDVNFIPTSTNNLENLADVSSQ 600

Query: 622 NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDY 681
           NNGDHGSM V+ S N+   AADTS+CKEAEKLAIQKILSDSYDSHQ  P E EEL+DFDY
Sbjct: 601 NNGDHGSMGVNPSKNVSDTAADTSNCKEAEKLAIQKILSDSYDSHQDFPCEPEELEDFDY 660

Query: 682 KNQAKDFGATKQGSPLMSSVANTSSVV-PMTSCNEARQSSSSYSNGGLEELEPAELVAPS 741
             QAKDFGATKQGSP+MSSVANTS VV P  SCNEARQSSSSYSNGGLEELEPAE+VAP 
Sbjct: 661 NKQAKDFGATKQGSPMMSSVANTSPVVLPTVSCNEARQSSSSYSNGGLEELEPAEIVAPL 720

Query: 742 STGTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           STGTAP  VA RKPIIRLSFTSLGKAGKSS
Sbjct: 721 STGTAP--VAERKPIIRLSFTSLGKAGKSS 747

BLAST of Cp4.1LG09g11290 vs. ExPASy TrEMBL
Match: A0A1S3CFS4 (Polynucleotide adenylyltransferase OS=Cucumis melo OX=3656 GN=LOC103500202 PE=3 SV=1)

HSP 1 Score: 1324 bits (3426), Expect = 0.0
Identity = 667/750 (88.93%), Postives = 699/750 (93.20%), Query Frame = 0

Query: 22  MGSPALNGRNNGQ-RLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEV 81
           MGSPAL GRNNGQ RLGITDPISLSGPTE DV+KTRELEKYLQDAGLYESQEEAV+REEV
Sbjct: 1   MGSPALCGRNNGQQRLGITDPISLSGPTEYDVLKTRELEKYLQDAGLYESQEEAVNREEV 60

Query: 82  LGRLDQIVKIWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 141
           LGRLDQIVKIWVKAISR+KGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA
Sbjct: 61  LGRLDQIVKIWVKAISRSKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHA 120

Query: 142 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLD 201
           TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVM+FK +GVSIDLLYAKLSLWVIPEDLD
Sbjct: 121 TREEDFFGELHKMLSEMPEVSELHPVPDAHVPVMRFKLSGVSIDLLYAKLSLWVIPEDLD 180

Query: 202 VSQDSILQNADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 261
           +SQ+SILQN DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF
Sbjct: 181 ISQESILQNTDEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGF 240

Query: 262 LGGINWALLVARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPR 321
           LGGINWALLVARICQL+PNALPNMLVSRFFRVFTQWRWPNPVMLC+NEEGSLGLPVWDPR
Sbjct: 241 LGGINWALLVARICQLYPNALPNMLVSRFFRVFTQWRWPNPVMLCANEEGSLGLPVWDPR 300

Query: 322 RNPKDRYHLMPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFE 381
           RNPKDRYHLMPIITPAYPCMNSSYNVS+STLRIMTEEFQRGHDICEVMEENKADWDTLFE
Sbjct: 301 RNPKDRYHLMPIITPAYPCMNSSYNVSASTLRIMTEEFQRGHDICEVMEENKADWDTLFE 360

Query: 382 PYPFFEAYKNYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFS 441
           PYPFFEAYKNYLQIDITAEN DD+R WKGWVESRLRQLTLKIERHTYN LQCHP+PGDFS
Sbjct: 361 PYPFFEAYKNYLQIDITAENDDDIRIWKGWVESRLRQLTLKIERHTYNMLQCHPYPGDFS 420

Query: 442 DKSKPFHHCYFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKK 501
           DKS+PFHHCYFMGLQRKQG PASG EQFD+RLTV+EFK SVN+YT  K GMEIYVSHVK+
Sbjct: 421 DKSRPFHHCYFMGLQRKQGGPASGSEQFDIRLTVDEFKRSVNVYTQRKRGMEIYVSHVKR 480

Query: 502 RNIPTFVFPGGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRL 561
           R+IP FVFPGGVRP RASK+TWD RRSSELKASDS QVD P EV ES+DG DDRRK++R+
Sbjct: 481 RSIPNFVFPGGVRPSRASKLTWDIRRSSELKASDSTQVDSPSEVTESLDG-DDRRKKIRI 540

Query: 562 DDNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQ 621
           DD+ANTN RNGECL   +SH EEVHEVSQVS TSSCSIKD+N  PTS NNLENLADVSSQ
Sbjct: 541 DDDANTNLRNGECLAAANSHHEEVHEVSQVSNTSSCSIKDVNFIPTSTNNLENLADVSSQ 600

Query: 622 NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDY 681
           NNGDHGSM V+ S N+   AADTS+CKEAEKLAIQKILSDSYDSHQ  P E EEL+DFDY
Sbjct: 601 NNGDHGSMGVNPSKNVSDTAADTSNCKEAEKLAIQKILSDSYDSHQDFPCEPEELEDFDY 660

Query: 682 KNQAKDFGATKQGSPLMSSVANTSSVV-PMTSCNEARQSSSSYSNGGLEELEPAELVAPS 741
             QAKDFGATKQGSP+MSSVANTS VV P  SCNEARQSSSSYSNGGLEELEPAE+VAP 
Sbjct: 661 NKQAKDFGATKQGSPMMSSVANTSPVVLPTVSCNEARQSSSSYSNGGLEELEPAEIVAPL 720

Query: 742 STGTAPAPVAARKPIIRLSFTSLGKAGKSS 769
           STGTAP  VA RKPIIRLSFTSLGKAGKSS
Sbjct: 721 STGTAP--VAERKPIIRLSFTSLGKAGKSS 747

BLAST of Cp4.1LG09g11290 vs. TAIR 10
Match: AT1G17980.1 (poly(A) polymerase 1 )

HSP 1 Score: 879.4 bits (2271), Expect = 2.8e-255
Identity = 472/744 (63.44%), Postives = 546/744 (73.39%), Query Frame = 0

Query: 30  RNNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVK 89
           + NGQR G+++PIS+ GPTE DVIKTRELEK+LQD GLYES+EEAV REEVLG LDQIVK
Sbjct: 5   QQNGQRFGVSEPISMGGPTEFDVIKTRELEKHLQDVGLYESKEEAVRREEVLGILDQIVK 64

Query: 90  IWVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGE 149
            W+K ISRAKGLN+QL+ EANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATRE DFFGE
Sbjct: 65  TWIKTISRAKGLNDQLLHEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREGDFFGE 124

Query: 150 LHKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQN 209
           L +MLSEMPEV+ELHPVPDAHVP+M FK  GVSIDLLYA+L LWVIPEDLD+SQDSILQN
Sbjct: 125 LQRMLSEMPEVTELHPVPDAHVPLMGFKLNGVSIDLLYAQLPLWVIPEDLDLSQDSILQN 184

Query: 210 ADEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 269
           ADEQTVRSLNGCRVTD+ILRLVPNIQ+FRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL
Sbjct: 185 ADEQTVRSLNGCRVTDQILRLVPNIQNFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALL 244

Query: 270 VARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHL 329
           VARICQL+PNALPN+LVSRFFRVF QW WPN + LCS +EGSLGL VWDPR NPKDR H+
Sbjct: 245 VARICQLYPNALPNILVSRFFRVFYQWNWPNAIFLCSPDEGSLGLQVWDPRINPKDRLHI 304

Query: 330 MPIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYK 389
           MPIITPAYPCMNSSYNVS STLRIM  EFQRG++ICE ME NKADWDTLFEP+ FFEAYK
Sbjct: 305 MPIITPAYPCMNSSYNVSESTLRIMKGEFQRGNEICEAMESNKADWDTLFEPFAFFEAYK 364

Query: 390 NYLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC 449
           NYLQIDI+A N DDLRKWKGWVESRLRQLTLKIERH +  L CHPHP DF D S+P H  
Sbjct: 365 NYLQIDISAANVDDLRKWKGWVESRLRQLTLKIERH-FKMLHCHPHPHDFQDTSRPLHCS 424

Query: 450 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 509
           YFMGLQRKQGVPA+ GEQFD+R TVEEFKH+VN YTLW PGMEI V H+K+R++P FVFP
Sbjct: 425 YFMGLQRKQGVPAAEGEQFDIRRTVEEFKHTVNAYTLWIPGMEISVGHIKRRSLPNFVFP 484

Query: 510 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEV---------AESIDGVDDRRKRMRL 569
           GGVRP   SK TWDS R SE + S ++                 A S   VD ++++   
Sbjct: 485 GGVRPSHTSKGTWDSNRRSEHRNSSTSSAPAATTTTTEMSSESKAGSNSPVDGKKRKWGD 544

Query: 570 DDNANTNSRNGECLVVVHSHPEEVHEVSQVSTTSSCSIKDLNSTPTSANNLENLADVSSQ 629
            +      RN + + V  S P E  E      + + S+  + S+P        + D  + 
Sbjct: 545 SETLTDQPRNSKHIAV--SVPVENCE----GGSPNPSVGSICSSP--------MKDYCT- 604

Query: 630 NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELDDFDY 689
            NG    +  D   N+V+ + D       E L I+KI +    +H+    ELEE   FD+
Sbjct: 605 -NGKSEPISKDPPENVVAFSKD-----PPESLPIEKIATP--QAHET--EELEE--SFDF 664

Query: 690 KNQAKDFGATKQGSPLMSSVANTSSVVPMTSCNEARQSSSSYSNGGLEELEPAELVAPSS 749
            NQ       +Q S  ++ ++ T+++ P     EA  + S +    +EELE      P +
Sbjct: 665 GNQ-----VIEQISHKVAVLSATATIPPF----EATSNGSPFPYEAVEELEVLPTRQPDA 709

Query: 750 TGTAPAPVAARKPIIRLSFTSLGK 765
                  V  RKPII+LSFTSLGK
Sbjct: 725 AHR--PSVQQRKPIIKLSFTSLGK 709

BLAST of Cp4.1LG09g11290 vs. TAIR 10
Match: AT4G32850.2 (nuclear poly(a) polymerase )

HSP 1 Score: 700.3 bits (1806), Expect = 2.4e-201
Identity = 358/660 (54.24%), Postives = 457/660 (69.24%), Query Frame = 0

Query: 31  NNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKI 90
           N+ +  GIT P+SL+GP+  D+ +  ELEKYL D GLYES+++ + REEVLGR+DQIVK 
Sbjct: 16  NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRIDQIVKH 75

Query: 91  WVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGEL 150
           WVK +++ +G  +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP +  REEDFF  L
Sbjct: 76  WVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFIIL 135

Query: 151 HKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNA 210
           H +L+EM EV+ELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLD+S  S+L   
Sbjct: 136 HDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCEV 195

Query: 211 DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLV 270
           DE TVRSLNGCRV D+IL+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLV
Sbjct: 196 DEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLV 255

Query: 271 ARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLM 330
           AR+CQL+PNA+P+MLVSRFFRV+TQWRWPNPVMLC+ EE  LG PVWD R+N +DRYHLM
Sbjct: 256 ARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHLM 315

Query: 331 PIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKN 390
           PIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK  W +LFE Y FFEAYKN
Sbjct: 316 PIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYKN 375

Query: 391 YLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC- 450
           YLQ+DI A +A+DL  WKGWVESR RQLTLKIER T   L CHP P ++ D ++ F HC 
Sbjct: 376 YLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHCA 435

Query: 451 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 510
           +FMGLQR +GV     +QFD+R TV+EF+  VNMY  WKPGM+++VSHV++R +P FVFP
Sbjct: 436 FFMGLQRAEGVGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLPPFVFP 495

Query: 511 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSR 570
            G R  R S+     +     K+ +   V     V E        R   R +D+   + R
Sbjct: 496 NGYRRPRQSR----HQNLPGGKSGEDGSVSHSGSVVE--------RHAKRKNDSEMMDVR 555

Query: 571 NGECLVVVHSHPEEVHEVSQVS-------TTSSCSIKDLNSTPTSANNLE----NLADVS 630
             +        P+ +  VS  +       T   C+++   S    A+NL      L D++
Sbjct: 556 PEKPEKRASLSPQSLDIVSPENSAITTGWTPPVCNLRRPPSEEIEADNLNTECTELTDLA 615

Query: 631 SQ--NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELD 677
               N+G    +EVD       + A    C +  +   + +  DS D    +  + E LD
Sbjct: 616 RNECNSGSEQVLEVD-------SMAVVQECSDPAEPLGKCVTPDSVDVVACVSGQEENLD 656

BLAST of Cp4.1LG09g11290 vs. TAIR 10
Match: AT4G32850.3 (nuclear poly(a) polymerase )

HSP 1 Score: 700.3 bits (1806), Expect = 2.4e-201
Identity = 358/660 (54.24%), Postives = 457/660 (69.24%), Query Frame = 0

Query: 31  NNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKI 90
           N+ +  GIT P+SL+GP+  D+ +  ELEKYL D GLYES+++ + REEVLGR+DQIVK 
Sbjct: 16  NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRIDQIVKH 75

Query: 91  WVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGEL 150
           WVK +++ +G  +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP +  REEDFF  L
Sbjct: 76  WVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFIIL 135

Query: 151 HKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNA 210
           H +L+EM EV+ELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLD+S  S+L   
Sbjct: 136 HDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCEV 195

Query: 211 DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLV 270
           DE TVRSLNGCRV D+IL+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLV
Sbjct: 196 DEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLV 255

Query: 271 ARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLM 330
           AR+CQL+PNA+P+MLVSRFFRV+TQWRWPNPVMLC+ EE  LG PVWD R+N +DRYHLM
Sbjct: 256 ARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHLM 315

Query: 331 PIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKN 390
           PIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK  W +LFE Y FFEAYKN
Sbjct: 316 PIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYKN 375

Query: 391 YLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC- 450
           YLQ+DI A +A+DL  WKGWVESR RQLTLKIER T   L CHP P ++ D ++ F HC 
Sbjct: 376 YLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHCA 435

Query: 451 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 510
           +FMGLQR +GV     +QFD+R TV+EF+  VNMY  WKPGM+++VSHV++R +P FVFP
Sbjct: 436 FFMGLQRAEGVGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLPPFVFP 495

Query: 511 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSR 570
            G R  R S+     +     K+ +   V     V E        R   R +D+   + R
Sbjct: 496 NGYRRPRQSR----HQNLPGGKSGEDGSVSHSGSVVE--------RHAKRKNDSEMMDVR 555

Query: 571 NGECLVVVHSHPEEVHEVSQVS-------TTSSCSIKDLNSTPTSANNLE----NLADVS 630
             +        P+ +  VS  +       T   C+++   S    A+NL      L D++
Sbjct: 556 PEKPEKRASLSPQSLDIVSPENSAITTGWTPPVCNLRRPPSEEIEADNLNTECTELTDLA 615

Query: 631 SQ--NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELD 677
               N+G    +EVD       + A    C +  +   + +  DS D    +  + E LD
Sbjct: 616 RNECNSGSEQVLEVD-------SMAVVQECSDPAEPLGKCVTPDSVDVVACVSGQEENLD 656

BLAST of Cp4.1LG09g11290 vs. TAIR 10
Match: AT4G32850.6 (nuclear poly(a) polymerase )

HSP 1 Score: 700.3 bits (1806), Expect = 2.4e-201
Identity = 358/660 (54.24%), Postives = 457/660 (69.24%), Query Frame = 0

Query: 31  NNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKI 90
           N+ +  GIT P+SL+GP+  D+ +  ELEKYL D GLYES+++ + REEVLGR+DQIVK 
Sbjct: 16  NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRIDQIVKH 75

Query: 91  WVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGEL 150
           WVK +++ +G  +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP +  REEDFF  L
Sbjct: 76  WVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFIIL 135

Query: 151 HKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNA 210
           H +L+EM EV+ELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLD+S  S+L   
Sbjct: 136 HDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCEV 195

Query: 211 DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLV 270
           DE TVRSLNGCRV D+IL+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLV
Sbjct: 196 DEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLV 255

Query: 271 ARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLM 330
           AR+CQL+PNA+P+MLVSRFFRV+TQWRWPNPVMLC+ EE  LG PVWD R+N +DRYHLM
Sbjct: 256 ARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHLM 315

Query: 331 PIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKN 390
           PIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK  W +LFE Y FFEAYKN
Sbjct: 316 PIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYKN 375

Query: 391 YLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC- 450
           YLQ+DI A +A+DL  WKGWVESR RQLTLKIER T   L CHP P ++ D ++ F HC 
Sbjct: 376 YLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHCA 435

Query: 451 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 510
           +FMGLQR +GV     +QFD+R TV+EF+  VNMY  WKPGM+++VSHV++R +P FVFP
Sbjct: 436 FFMGLQRAEGVGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLPPFVFP 495

Query: 511 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSR 570
            G R  R S+     +     K+ +   V     V E        R   R +D+   + R
Sbjct: 496 NGYRRPRQSR----HQNLPGGKSGEDGSVSHSGSVVE--------RHAKRKNDSEMMDVR 555

Query: 571 NGECLVVVHSHPEEVHEVSQVS-------TTSSCSIKDLNSTPTSANNLE----NLADVS 630
             +        P+ +  VS  +       T   C+++   S    A+NL      L D++
Sbjct: 556 PEKPEKRASLSPQSLDIVSPENSAITTGWTPPVCNLRRPPSEEIEADNLNTECTELTDLA 615

Query: 631 SQ--NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELD 677
               N+G    +EVD       + A    C +  +   + +  DS D    +  + E LD
Sbjct: 616 RNECNSGSEQVLEVD-------SMAVVQECSDPAEPLGKCVTPDSVDVVACVSGQEENLD 656

BLAST of Cp4.1LG09g11290 vs. TAIR 10
Match: AT4G32850.5 (nuclear poly(a) polymerase )

HSP 1 Score: 700.3 bits (1806), Expect = 2.4e-201
Identity = 358/660 (54.24%), Postives = 457/660 (69.24%), Query Frame = 0

Query: 31  NNGQRLGITDPISLSGPTEDDVIKTRELEKYLQDAGLYESQEEAVSREEVLGRLDQIVKI 90
           N+ +  GIT P+SL+GP+  D+ +  ELEKYL D GLYES+++ + REEVLGR+DQIVK 
Sbjct: 16  NSPKSYGITKPLSLAGPSSADIKRNVELEKYLVDEGLYESKDDTMRREEVLGRIDQIVKH 75

Query: 91  WVKAISRAKGLNEQLVQEANAKIFTFGSYRLGVHGPGADIDTLCVGPRHATREEDFFGEL 150
           WVK +++ +G  +Q+V++ANA IFTFGSYRLGVHGPGADIDTLCVGP +  REEDFF  L
Sbjct: 76  WVKQLTQQRGYTDQMVEDANAVIFTFGSYRLGVHGPGADIDTLCVGPSYVNREEDFFIIL 135

Query: 151 HKMLSEMPEVSELHPVPDAHVPVMKFKFAGVSIDLLYAKLSLWVIPEDLDVSQDSILQNA 210
           H +L+EM EV+ELHPVPDAHVPVMKFKF G+ IDLLYA +SL V+P+DLD+S  S+L   
Sbjct: 136 HDILAEMEEVTELHPVPDAHVPVMKFKFQGIPIDLLYASISLLVVPQDLDISSSSVLCEV 195

Query: 211 DEQTVRSLNGCRVTDRILRLVPNIQSFRTTLRCMRFWAKRRGVYSNVSGFLGGINWALLV 270
           DE TVRSLNGCRV D+IL+LVPN + FRTTLRC+++WAK+RGVYSNV+GFLGG+NWALLV
Sbjct: 196 DEPTVRSLNGCRVADQILKLVPNFEHFRTTLRCLKYWAKKRGVYSNVTGFLGGVNWALLV 255

Query: 271 ARICQLFPNALPNMLVSRFFRVFTQWRWPNPVMLCSNEEGSLGLPVWDPRRNPKDRYHLM 330
           AR+CQL+PNA+P+MLVSRFFRV+TQWRWPNPVMLC+ EE  LG PVWD R+N +DRYHLM
Sbjct: 256 ARVCQLYPNAIPSMLVSRFFRVYTQWRWPNPVMLCAIEEDELGFPVWDRRKNHRDRYHLM 315

Query: 331 PIITPAYPCMNSSYNVSSSTLRIMTEEFQRGHDICEVMEENKADWDTLFEPYPFFEAYKN 390
           PIITPAYPCMNSSYNVS STLR+MTE+FQ G++I + +E NK  W +LFE Y FFEAYKN
Sbjct: 316 PIITPAYPCMNSSYNVSQSTLRVMTEQFQFGNNILQEIELNKQHWSSLFEQYMFFEAYKN 375

Query: 391 YLQIDITAENADDLRKWKGWVESRLRQLTLKIERHTYNKLQCHPHPGDFSDKSKPFHHC- 450
           YLQ+DI A +A+DL  WKGWVESR RQLTLKIER T   L CHP P ++ D ++ F HC 
Sbjct: 376 YLQVDIVAADAEDLLAWKGWVESRFRQLTLKIERDTNGMLMCHPQPNEYVDTARQFLHCA 435

Query: 451 YFMGLQRKQGVPASGGEQFDMRLTVEEFKHSVNMYTLWKPGMEIYVSHVKKRNIPTFVFP 510
           +FMGLQR +GV     +QFD+R TV+EF+  VNMY  WKPGM+++VSHV++R +P FVFP
Sbjct: 436 FFMGLQRAEGVGGQECQQFDIRGTVDEFRQEVNMYMFWKPGMDVFVSHVRRRQLPPFVFP 495

Query: 511 GGVRPLRASKVTWDSRRSSELKASDSAQVDRPFEVAESIDGVDDRRKRMRLDDNANTNSR 570
            G R  R S+     +     K+ +   V     V E        R   R +D+   + R
Sbjct: 496 NGYRRPRQSR----HQNLPGGKSGEDGSVSHSGSVVE--------RHAKRKNDSEMMDVR 555

Query: 571 NGECLVVVHSHPEEVHEVSQVS-------TTSSCSIKDLNSTPTSANNLE----NLADVS 630
             +        P+ +  VS  +       T   C+++   S    A+NL      L D++
Sbjct: 556 PEKPEKRASLSPQSLDIVSPENSAITTGWTPPVCNLRRPPSEEIEADNLNTECTELTDLA 615

Query: 631 SQ--NNGDHGSMEVDHSTNIVSAAADTSSCKEAEKLAIQKILSDSYDSHQALPSELEELD 677
               N+G    +EVD       + A    C +  +   + +  DS D    +  + E LD
Sbjct: 616 RNECNSGSEQVLEVD-------SMAVVQECSDPAEPLGKCVTPDSVDVVACVSGQEENLD 656