Cp4.1LG09g09050 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g09050
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Tryptophan synthase
Location	Cp4.1LG09: 8152633 .. 8157114 (+)
RNA-Seq Expression	Cp4.1LG09g09050
Synteny	Cp4.1LG09g09050

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTCCGTGTTCACGTTCCCATGAAATTGACATCTACTCTTCTTTGCCAACCCTAATTTTCTAGGGAAAATGAATTAAGTGGATTTGGTACTAATTTATTAAAGACTTCAATATCAACGTTAGCCAAACTAAGAACGAAATTGATTATTAAAAAAAATTATTTAATTTCAGTGAGTACGAATTGTTTAATAAGAACGTCATAATGTAACTGTCTCTTAATAATCGTCGGAGGTAGATCCAAAAATATATTTATTATTTAATAATTATTAGTTATAAAAGTGAAATATTTTTACCATTTATCTTTTATTCATCGAGCTATCAATCATATAATAGCTTTGCTTTCGAATAACCTTGAGCCATATTGGACCAAACAACTATTTGTTGCGTATCTAATTTCTATAGCTATTACTTTTCCCTTTTTTTTTCCTTGCAAAACATGTGCAATAAGATGAATCAATTCACTCTCTATATAAACAACCCTTGTGTGCCTCTCTTAACACAAACCACTCAACTCCCTCCCTCTCTCTCTCTCTCAATTGTTAATACTCAAAGGGAAGAACATACCTAGAAATGGCATCCAATATTGTGCACAACATTGCTGCTGCTGTCACTAACCAATTAGATGCAACCACAGGCATAGCCAGGTCGCATGTAAAGCTTGGCAACGGAATTGGGTCACCCACTTTCACGCCAACAAGGAAGCCTACGCCAGCATTAAAGATGCAGCCTGTAATTGATGATCACCCCAAGAACTCTAAACAAACAATTCATGAAGGAAAGTTTGGGAAATTTGGTGGGAAGTTTGTGCCCGAGTCTCTAATCACTTGCTTGGCCAAATTGGAAGCCGAGTTCAACTTGGTTTTGAACGATTCCAAATTTCAGGTATGCGTTCTTTTCATAAATTATGTTGAGGATTGTTGGGGGAGGAGTCCTATATCGACTAATTAAGGGGTTGATCATGGTACGAGGCCTTTCGGGAAACAAAAAGTAAAGCCACGAGAGCTTATGCTCAAAGTGGATAATATCATACCATTGTAGAGATCGTGATTTCTAACATGGTATTACAGTCATGCCCTTAACTTAGTCATGTCAATAGAATCCTCAAATGTCGAACAAAAAAGTTGTGAGCCTCAAATGTCGAACAAAAAAGTTATGAGCCTCAAATGTGTAGTCAAAAGTGACTCCAGTGTCGAACAAAGGATGTATTTTGTTCGAGAGCTCCATAGAACGAGTCAAGATTTGATTAAGGAGAGGCTGTTTGAAGGTTCCATAGGCCTCAGGGGAGGCTCTATGGTGTATTTTATTCGAGAGAAGGATTGTGCTCAAAATGGACATTATCATACTATTACGAAGAGTCGTGATTCCTAATAAAGTATTAGTGAATTAATTTTGGGTGTTGTGGTTGTGCAGGAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAAGGCACTACAAAGACGAGGAAGGAAAAGGTCCAGAGATTTACATAAAAAGAGAGGATTTGAACCACTGCGGTGCACACAAGATGAACAACGCAATAGCGCAGGTTATGATTGCCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTGGGGCTGGCCAGCATGGTGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACTATTTTCATGGGCTCTGAAGATATCAAGAAACAATCCTCCAATGTTGTCTTAATCAAGCTGCTCGGTGCTCAGGTACTCCATTAATTTGTCGAGTATTGTTGTGAGGGAACTAATTTAGGGAATGATCATGAGTTTATAAGTAAAGAAGGGAAGCCCAAAGCTCACGAGAGCTTATGCTGAAAATGGACAATATCATGTCATTGTGGAGATTCGTGATTCCTAACAGATTTAATATCTCAGGTGAAATCTGTAGAAGGAAACTTCAAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGAACTTGGAAATGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCAATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGAGGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATGAACGAAGAAGATGTGCGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGCTATCTTCTCCAAGATGATGAAGGCCAAATCCTTGTCCCTCATTCCGTCGGTGTAGGGTAAGTATGTTAGAAATCACGAACTTCCATAATATTTTCATATTATCCACTTTAGGTGGAGATTTCTCGACGCGCATCATACCATACCAATGTAGATGTATTCCTTATTTATAAACTCATTATTAATCCCTTAATTAATCGACGTAGAACTCCTCTCTCAACAATCCTCAACCATCCTCCCTTCGAATAAAGTACACTATAGAGCCTCCCCTAAATCCTATGGAGCCTTCGAACAGTCTCCCCTTAATCGAGACTTGACTTCTTCTCTAGAGCCCTTAACAATCCTCCCTTCGAATAAAGTACACCATAGAGCCTCCCCTGATATCTATGGAGCCCTTGAACAACTTCTCGCCTTAATCGAGGCTCGATTCCTTCTCTGAAACACTCAAATAAAGTACACCCTTTATTCGATATTTCAGTCACTTTTGACTACACTTTCGAGAGTCACAAGTTCTTTATTCAACATTTGAGGATTCTATTGACATGACTAAGTTCAGGGCATGACTCCAATACCATGTTAGAAATAACGAACCTCCATAATTGTATGATATTGTCCACTTTGAGCATTAGCTCTCGTGACTCTACTTTTGGTTTCTCCAAAAGACTTCATAATACCAATGCATATGTATTCCTTGCTTATAAACTTATGATCAACCCTTTAATTAGTCGACGACGCGAGACTACTCTCCCACCAATCCTCACTAGAGTATTTTAAATTTCGAACTTTTTTGAATTGCAGGCTGGAATACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGCAGAGCTGAATTTCACACAGCAATAGACAAAGAAGCAGTGGAAGTGTTGAGATATATTATTTATGGAATATATCCTAGATATTATATCTTAATATTCTTCCATCTATGGTTAGATTTATAGTATATTTTATTTTATTCTAGTATTTAATTATTTTATATTTTCCTCATTTGTACCGGCTTGTATCCTATTTAAAGGATATCAATATCAATGATAATATACACCTTCCCAATTATATTTTCTATCTCATTCTTAACATGGTATCAGAGCACCGATCTTGATATTCCTAATATCAAAGCATCGATCGTGGTATTCCTAATACCCCCTTGGACTCTGAGTCCAAGGGGAGTATTACCTAAAGCCAAACCCTAACCAATCAGTGAAGTCTTTACCATCGTCAATCATCCAATATCAAGAATACGTTATAAGCATCTTCAAATTTCTCACCAAGCTCTAAGGTGACANATCGATCATTTGCAAATATGGCCGATGTTTCTCAGTTGAATACAGAAGAACAGTCGCCTAATTCCATCCCAAATTTTTCTTCTGGGCAATTACAACAGATTGTACAAGCCTTATCTGCACTCAATCATCGTCCTTTTAGTAATTCTGATAATAACATCAATGTTGCAGGTTTGTTTCCAGTATCTGCATTATCTATTAACTCTACGAGTTCTAATCCATGGATTCTTGATAGTGGAGCTACAGATCATATAGTATCCAAAGCTTCTGTTATCACTGAACCAAAGGCTACCACCATATCTACAATAAATTTGCCTAATGGAGGGACAGCACATGTGTCACATACCGGCAATGTTTCTTTTAATCCTGACCTTAAATTAAACAATGTTTTATGTGTGCCTTCATTCAACTTAAACCTAATGTCTGTCAGCAAACTTACCAATGACTTGAAATGTTATGTGACCTTTTATCCTGATTCTTGTGTTATGCAGGACTTGGCTACGGGGAAGATGATTGGCTCGGGTAAACAATCCGGAGGTCTCTATCATATTTCTTCATCTCCAATCAAGCCTTCAGCAAATCAAGTATCTCAGTCATCTGATTTGTGGCATTTACGCCTAGGTCATCCTTCTCCTTCTCGTTTTAAATTTCTAGCTGATCAATTGCATCTTACAAACGGGTATGCAAATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTCGACAAGCTTTGCCCCACTTTGCGCGACGGCTGCAAGGTGGTGGTCAACTGCAGTGGCCGTGGTGACAAAGACGCTGCCATAGTCTTCAATTATCAACCCTATCAAGAACACAACATATTTTAA

mRNA sequence

ATGGTCCGTGGAAGAACATACCTAGAAATGGCATCCAATATTGTGCACAACATTGCTGCTGCTGTCACTAACCAATTAGATGCAACCACAGGCATAGCCAGGTCGCATGTAAAGCTTGGCAACGGAATTGGGTCACCCACTTTCACGCCAACAAGGAAGCCTACGCCAGCATTAAAGATGCAGCCTGTAATTGATGATCACCCCAAGAACTCTAAACAAACAATTCATGAAGGAAAGTTTGGGAAATTTGGTGGGAAGTTTGTGCCCGAGTCTCTAATCACTTGCTTGGCCAAATTGGAAGCCGAGCACTACAAAGACGAGGAAGGAAAAGGTCCAGAGATTTACATAAAAAGAGAGGATTTGAACCACTGCGGTGCACACAAGATGAACAACGCAATAGCGCAGGTTATGATTGCCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTGGGGCTGGCCAGCATGGTGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGACTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCAATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGAGGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATGAACGAAGAAGATGTGCGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGCTTTGCCCCACTTTGCGCGACGGCTGCAAGGTGGTGGTCAACTGCAGTGGCCGTGGTGACAAAGACGCTGCCATAGTCTTCAATTATCAACCCTATCAAGAACACAACATATTTTAA

Coding sequence (CDS)

ATGGTCCGTGGAAGAACATACCTAGAAATGGCATCCAATATTGTGCACAACATTGCTGCTGCTGTCACTAACCAATTAGATGCAACCACAGGCATAGCCAGGTCGCATGTAAAGCTTGGCAACGGAATTGGGTCACCCACTTTCACGCCAACAAGGAAGCCTACGCCAGCATTAAAGATGCAGCCTGTAATTGATGATCACCCCAAGAACTCTAAACAAACAATTCATGAAGGAAAGTTTGGGAAATTTGGTGGGAAGTTTGTGCCCGAGTCTCTAATCACTTGCTTGGCCAAATTGGAAGCCGAGCACTACAAAGACGAGGAAGGAAAAGGTCCAGAGATTTACATAAAAAGAGAGGATTTGAACCACTGCGGTGCACACAAGATGAACAACGCAATAGCGCAGGTTATGATTGCCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTGGGGCTGGCCAGCATGGTGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGACTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCAATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGAGGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATGAACGAAGAAGATGTGCGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGCTTTGCCCCACTTTGCGCGACGGCTGCAAGGTGGTGGTCAACTGCAGTGGCCGTGGTGACAAAGACGCTGCCATAGTCTTCAATTATCAACCCTATCAAGAACACAACATATTTTAA

Protein sequence

MVRGRTYLEMASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPKNSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLDYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGLFNEFMNEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQPYQEHNIF

Homology

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BLAST of Cp4.1LG09g09050 vs. ExPASy Swiss-Prot
Match: O50046 (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=16922 GN=TSB PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.4e-65
Identity = 147/382 (38.48%), Postives = 183/382 (47.91%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLEAE----------------------------------- 137
           G+FGKFGGK+VPE+L+  L +LE+                                    
Sbjct: 75  GRFGKFGGKYVPETLMYALTELESAFRSLSGDQVFQKELDGILKDYVGRESPLYFAERLT 134

Query: 138 -HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATA 197
            HYK   G+GPEIY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA
Sbjct: 135 LHYKRPNGEGPEIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATA 194

Query: 198 AACAKHDLD--------------------------------------------------- 257
             CA+  L                                                    
Sbjct: 195 TVCARFGLQCVIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLKDATSEAIRDWVTN 254

Query: 258 ----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGL 286
               +Y+ G+V GPHP P MVREF +VIGKETRKQA+EKWGG P+VLVAC+G GSNA+GL
Sbjct: 255 VESTHYILGSVAGPHPYPMMVREFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGL 314

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy Swiss-Prot
Match: P14671 (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 4.5e-65
Identity = 147/389 (37.79%), Postives = 186/389 (47.81%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLEA------------------------------------ 137
           G+FGKFGGK+VPE+L+  L++LE+                                    
Sbjct: 79  GRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVGRESPLYFAERLT 138

Query: 138 EHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATA 197
           EHY+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA
Sbjct: 139 EHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATA 198

Query: 198 AACAKHDLD--------------------------------------------------- 257
             CA+  L+                                                   
Sbjct: 199 TVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKDATSEAIRDWVTN 258

Query: 258 ----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGL 293
               +Y+ G+V GPHP P MVR+F +VIGKETRKQA+EKWGG P+VLVAC+G GSNA+GL
Sbjct: 259 VETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGL 318

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy Swiss-Prot
Match: P25269 (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB2 PE=1 SV=2)

HSP 1 Score: 246.9 bits (629), Expect = 2.9e-64
Identity = 146/389 (37.53%), Postives = 183/389 (47.04%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLE------------------------------------A 137
           G+FGKFGGK+VPE+L+  L++LE                                     
Sbjct: 84  GRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEILKDYVGRESPLYFAERLT 143

Query: 138 EHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATA 197
           EHY+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA
Sbjct: 144 EHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATA 203

Query: 198 AACAKHDLD--------------------------------------------------- 257
             CA+  L                                                    
Sbjct: 204 TVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKDATSEAIRDWVTN 263

Query: 258 ----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGL 293
               +Y+ G+V GPHP P MVR+F +VIGKETRKQAMEKWGG P+VLVAC+G GSNA+GL
Sbjct: 264 VETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGKPDVLVACVGGGSNAMGL 323

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy Swiss-Prot
Match: P43284 (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 GN=TSB2 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.1e-62
Identity = 141/389 (36.25%), Postives = 179/389 (46.02%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLEA------------------------------------ 137
           G+FG+FGGK+VPE+L+  L +LE+                                    
Sbjct: 52  GRFGRFGGKYVPETLMHALTELESAFHALATDDEFQKELDGILKDYVGRESPLYFAERLT 111

Query: 138 EHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATA 197
           EHYK  +G GP IY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA
Sbjct: 112 EHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATA 171

Query: 198 AACAKHDLD--------------------------------------------------- 257
             C +  L                                                    
Sbjct: 172 TVCRRFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRAVHSGTATLKDATSEAIRDWVTN 231

Query: 258 ----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGL 293
               +Y+ G+V GPHP P MVREF  VIGKETR+QAM+KWGG P+VLVAC+G GSNA+GL
Sbjct: 232 VETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQAMDKWGGKPDVLVACVGGGSNAMGL 291

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy Swiss-Prot
Match: P43283 (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.5e-60
Identity = 141/386 (36.53%), Postives = 175/386 (45.34%), Query Frame = 0


Query: 81  GKFGGKFVPESLITCLAKLE------------------------------------AEHY 140
           G+FGGK+VPE+L+  L +LE                                     EHY
Sbjct: 1   GRFGGKYVPETLMHALTELENAFHALATDDEFQKELDGILKDYVGRESPLYFAERLTEHY 60

Query: 141 KDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAAC 200
           K  +G GP IY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA  C
Sbjct: 61  KRADGTGPLIYLKREDLNHRGAHKINNAVAQALLAKRLGKQRIIAETGAGQHGVATATVC 120

Query: 201 AKHDLD------------------------------------------------------ 260
           A+  L                                                       
Sbjct: 121 ARFGLQCIIYMGAQDMERQALNVFRMKLLGAEVRAVHSGTATLKDATSEAIRDWVTNVET 180

Query: 261 -YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGLFNE 293
            +Y+ G+V GPHP P MVREF  VIGKETR+QAM KWGG P+VLVAC+G GSNA+GLF+E
Sbjct: 181 THYILGSVAGPHPYPMMVREFHKVIGKETRRQAMHKWGGKPDVLVACVGGGSNAMGLFHE 240

[Close]

BLAST of Cp4.1LG09g09050 vs. NCBI nr
Match: XP_023542665.1 (tryptophan synthase beta chain 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 494 bits (1273), Expect = 3.79e-172
Identity = 281/459 (61.22%), Postives = 284/459 (61.87%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASNIVHNIAAAVTNQLDAT GIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK
Sbjct: 1   MASNIVHNIAAAVTNQLDATRGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    +YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 297
                    YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS
Sbjct: 241 EWVGNLEMSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. NCBI nr
Match: XP_022954968.1 (tryptophan synthase beta chain 1-like [Cucurbita moschata])

HSP 1 Score: 487 bits (1253), Expect = 4.14e-169
Identity = 276/459 (60.13%), Postives = 282/459 (61.44%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASN+VHNI AAVTNQLDAT GIARSHVKLGNGIGSPTFTPT+KPTPALKMQPVIDDH K
Sbjct: 1   MASNVVHNINAAVTNQLDATRGIARSHVKLGNGIGSPTFTPTKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYYAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 297
                    YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGNLERSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. NCBI nr
Match: KAG6573205.1 (Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 486 bits (1252), Expect = 5.88e-169
Identity = 277/459 (60.35%), Postives = 282/459 (61.44%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASNIVHNIAAAVTNQLDAT GIARSHVKLGNGIGSPTFTPT+KPTPALKMQPVIDDH K
Sbjct: 1   MASNIVHNIAAAVTNQLDATRGIARSHVKLGNGIGSPTFTPTKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    +YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 297
                    YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGNLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. NCBI nr
Match: XP_022954970.1 (tryptophan synthase beta chain 1-like [Cucurbita moschata])

HSP 1 Score: 478 bits (1229), Expect = 1.44e-165
Identity = 274/454 (60.35%), Postives = 279/454 (61.45%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASNIVHNIAA VTNQLDAT GIARSHVKLGNGIGSPTFTPT+KPTPALKMQPVIDDH K
Sbjct: 1   MASNIVHNIAA-VTNQLDATRGIARSHVKLGNGIGSPTFTPTKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    +YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 292
                    YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. NCBI nr
Match: XP_022994295.1 (tryptophan synthase beta chain 1-like [Cucurbita maxima])

HSP 1 Score: 474 bits (1221), Expect = 2.37e-164
Identity = 272/454 (59.91%), Postives = 277/454 (61.01%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASNIVHNIAA +TNQLDAT GIARSHVKLGNGIGSPTFTP +KPTPALKMQPVIDDH K
Sbjct: 1   MASNIVHNIAA-LTNQLDATRGIARSHVKLGNGIGSPTFTPAKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYHAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLINLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 292
                    YYL GTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGNLEMSYYLMGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy TrEMBL
Match: A0A6J1GSL9 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1)

HSP 1 Score: 487 bits (1253), Expect = 2.01e-169
Identity = 276/459 (60.13%), Postives = 282/459 (61.44%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASN+VHNI AAVTNQLDAT GIARSHVKLGNGIGSPTFTPT+KPTPALKMQPVIDDH K
Sbjct: 1   MASNVVHNINAAVTNQLDATRGIARSHVKLGNGIGSPTFTPTKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYYAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 297
                    YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGNLERSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy TrEMBL
Match: A0A6J1GTV6 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457069 PE=3 SV=1)

HSP 1 Score: 478 bits (1229), Expect = 6.98e-166
Identity = 274/454 (60.35%), Postives = 279/454 (61.45%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASNIVHNIAA VTNQLDAT GIARSHVKLGNGIGSPTFTPT+KPTPALKMQPVIDDH K
Sbjct: 1   MASNIVHNIAA-VTNQLDATRGIARSHVKLGNGIGSPTFTPTKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    +YKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 292
                    YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy TrEMBL
Match: A0A6J1K0T4 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1)

HSP 1 Score: 474 bits (1221), Expect = 1.15e-164
Identity = 272/454 (59.91%), Postives = 277/454 (61.01%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTFTPTRKPTPALKMQPVIDDHPK 69
           MASNIVHNIAA +TNQLDAT GIARSHVKLGNGIGSPTFTP +KPTPALKMQPVIDDH K
Sbjct: 1   MASNIVHNIAA-LTNQLDATRGIARSHVKLGNGIGSPTFTPAKKPTPALKMQPVIDDHHK 60

Query: 70  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE--------------------------- 129
           NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAE                           
Sbjct: 61  NSKQTIHEGKFGKFGGKFVPESLITCLAKLEAEFNLVLNDSKFQEELEVALRDFVGRETP 120

Query: 130 ---------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 189
                    HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA
Sbjct: 121 LYHAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGA 180

Query: 190 GQHGVATAAACAKHDLD------------------------------------------- 249
           GQHGVATAAACAKHDL+                                           
Sbjct: 181 GQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLINLLGAQVKSVEGNFKDASSEAIR 240

Query: 250 ---------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGS 292
                    YYL GTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLP+VLVACIGSGS
Sbjct: 241 EWVGNLEMSYYLMGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGS 300

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy TrEMBL
Match: A0A0A0LUA1 (Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G064170 PE=3 SV=1)

HSP 1 Score: 368 bits (945), Expect = 8.85e-123
Identity = 229/460 (49.78%), Postives = 247/460 (53.70%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGNGIGSPTF--TPTRKPTPALKMQPVIDDH 69
           MA N+V+N  AA+TNQL     +A+SHV +G     P    T  +     +KMQ VIDD 
Sbjct: 1   MACNLVNN--AAITNQL-----VAKSHVNVGKLGTGPNILATANKNYMRTIKMQLVIDDP 60

Query: 70  PKNSKQTIHE-GKFGKFGGKFVPESLITCLAKLEAE------------------------ 129
            K+      E GKFGKFGGKFVPESLITCL KLEAE                        
Sbjct: 61  KKSQNLGFGELGKFGKFGGKFVPESLITCLGKLEAEFNLVLNDSKFQEELEVALRDFVGR 120

Query: 130 ------------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAA 189
                       HYK+EEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGR+ VVAA
Sbjct: 121 ETPLYYAERLTKHYKNEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRKSVVAA 180

Query: 190 TGAGQHGVATAAACAKHDLD---------------------------------------- 249
           TGAGQHGVATAAACAKHDLD                                        
Sbjct: 181 TGAGQHGVATAAACAKHDLDCTIFMGTEDIKKQSSNVLLIKMLGAKVKAVEGNFKDASSE 240

Query: 250 ------------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIG 295
                       YYLTGTVVGPHPCPAMVREFQSVIGKETR+QAMEKWG  P+VL+ACIG
Sbjct: 241 AIRGWVGNLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRRQAMEKWGAKPDVLLACIG 300

[Close]

BLAST of Cp4.1LG09g09050 vs. ExPASy TrEMBL
Match: A0A1S3B3A1 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1)

HSP 1 Score: 359 bits (922), Expect = 2.67e-119
Identity = 228/463 (49.24%), Postives = 248/463 (53.56%), Query Frame = 0


Query: 10  MASNIVHNIAAAVTNQLDATTGIARSHVKLGN-GIGSPTFTPTRKP-TPALKMQPVIDDH 69
           MA N+V N  +A+TNQL     +A+ HV  G  G G+ T   T++     +KMQ V D+ 
Sbjct: 1   MACNMVKN--SAITNQL-----VAKPHVNFGKLGHGANTLATTKRNYMGTIKMQLVTDNP 60

Query: 70  PKNSKQTIHE-GKFGKFGGKFVPESLITCLAKLEAE------------------------ 129
            K+    I E GKFGKFGGKFVPESLITCL KLEAE                        
Sbjct: 61  KKSQNLGIGELGKFGKFGGKFVPESLITCLGKLEAEFNLVLNDSKFQEELEVALRDFVGR 120

Query: 130 ------------HYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAA 189
                       HYK+EEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGR+ VVAA
Sbjct: 121 ETPLYYAERLTKHYKNEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRKSVVAA 180

Query: 190 TGAGQHGVATAAACAKHDLD---------------------------------------- 249
           TGAGQHGVATAAACAKHDLD                                        
Sbjct: 181 TGAGQHGVATAAACAKHDLDCTIFMGSEDINKQSSNVLLIKLLGAKVKSVEGNFKDASSE 240

Query: 250 ------------YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIG 298
                       YYLTGTVVGP+PCPAMVREFQSVIGKETR+QA EKWG  P+VL+ACIG
Sbjct: 241 AIREWVGNLETSYYLTGTVVGPYPCPAMVREFQSVIGKETRRQAKEKWGAKPDVLLACIG 300

[Close]

BLAST of Cp4.1LG09g09050 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.5e-68
Identity = 162/383 (42.30%), Postives = 188/383 (49.09%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLE------------------------------------A 137
           GKFG+FGGKFVPE+L++ L +LE                                     
Sbjct: 70  GKFGRFGGKFVPETLMSRLIELEDEFNFVRCDHEFQEELTTALRDYVGRETPLYFAERLT 129

Query: 138 EHYKD----EEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHG 197
           EHYK+     EG GPEIY+KREDL+HCG+HK+NNA+AQ MI++R+G  RVVAATGAGQHG
Sbjct: 130 EHYKNIVPTIEG-GPEIYLKREDLSHCGSHKINNALAQAMISRRLGCSRVVAATGAGQHG 189

Query: 198 VATAAACAKHDLD----------------------------------------------- 257
           VATAAACAK  L+                                               
Sbjct: 190 VATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLLGAQVISVEGTFKDASSEAIRNWVE 249

Query: 258 -----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALG 286
                YYL+GTVVGPHPCP +VREFQSVIGKETR+QA + WGG P+VLVAC+GSGSNALG
Sbjct: 250 NLYTTYYLSGTVVGPHPCPIIVREFQSVIGKETRRQAKQLWGGKPDVLVACVGSGSNALG 309

[Close]

BLAST of Cp4.1LG09g09050 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 249.6 bits (636), Expect = 3.2e-66
Identity = 147/389 (37.79%), Postives = 186/389 (47.81%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLEA------------------------------------ 137
           G+FGKFGGK+VPE+L+  L++LE+                                    
Sbjct: 79  GRFGKFGGKYVPETLMHALSELESAFYALATDDDFQRELAGILKDYVGRESPLYFAERLT 138

Query: 138 EHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATA 197
           EHY+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA
Sbjct: 139 EHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATA 198

Query: 198 AACAKHDLD--------------------------------------------------- 257
             CA+  L+                                                   
Sbjct: 199 TVCARFGLECIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKDATSEAIRDWVTN 258

Query: 258 ----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGL 293
               +Y+ G+V GPHP P MVR+F +VIGKETRKQA+EKWGG P+VLVAC+G GSNA+GL
Sbjct: 259 VETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQALEKWGGKPDVLVACVGGGSNAMGL 318

[Close]

BLAST of Cp4.1LG09g09050 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 246.9 bits (629), Expect = 2.1e-65
Identity = 146/389 (37.53%), Postives = 183/389 (47.04%), Query Frame = 0


Query: 78  GKFGKFGGKFVPESLITCLAKLE------------------------------------A 137
           G+FGKFGGK+VPE+L+  L++LE                                     
Sbjct: 84  GRFGKFGGKYVPETLMHALSELETAFYSLATDEDFQRELAEILKDYVGRESPLYFAERLT 143

Query: 138 EHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATA 197
           EHY+ E G+GP IY+KREDLNH GAHK+NNA+AQ ++AKR+G++R++A TGAGQHGVATA
Sbjct: 144 EHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQALLAKRLGKKRIIAETGAGQHGVATA 203

Query: 198 AACAKHDLD--------------------------------------------------- 257
             CA+  L                                                    
Sbjct: 204 TVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAEVRGVHSGTATLKDATSEAIRDWVTN 263

Query: 258 ----YYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGL 293
               +Y+ G+V GPHP P MVR+F +VIGKETRKQAMEKWGG P+VLVAC+G GSNA+GL
Sbjct: 264 VETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQAMEKWGGKPDVLVACVGGGSNAMGL 323

[Close]

BLAST of Cp4.1LG09g09050 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 47.4 bits (111), Expect = 2.4e-05
Identity = 45/184 (24.46%), Postives = 66/184 (35.87%), Query Frame = 0


Query: 114 IYIKREDLNHCGAHKMNNAIAQVMIAKRMGRRRVVAATGAGQHGVATAAACAKHDLDYYL 173
           IY K E  +  G+HK N A+ Q     + G + VV  TGAGQ G + A A +   LD  +
Sbjct: 152 IYFKYEGGSPAGSHKPNTAVPQAYYNAKEGVKNVVTETGAGQWGSSLAFASSLFGLDCEV 211

Query: 174 TGTV-----------------VGPHPCPAMVRE--------------------------- 233
                                   HP P+ + E                           
Sbjct: 212 WQVANSYHTKPYRRLMMQTWGAKVHPSPSDLTEAGRRILESDPSSPGSLGIAISEAVEVA 271

Query: 234 -------------------FQSVIGKETRKQAMEKWGGLPEVLVACIGSGSNALGLFNEF 235
                               Q++IG+E  +Q ME +G  P++++ C G GSN  GL   F
Sbjct: 272 ARNEDTKYCLGSVLNHVLLHQTIIGEECIQQ-MENFGETPDLIIGCTGGGSNFAGLSFPF 331

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g09050 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O50046	3.4e-65	38.48	Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=1692...	[more]
P14671	4.5e-65	37.79	Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P25269	2.9e-64	37.53	Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P43284	6.1e-62	36.25	Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 G...	[more]
P43283	2.5e-60	36.53	Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=...	[more]

BLAST of Cp4.1LG09g09050 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023542665.1	3.79e-172	61.22	tryptophan synthase beta chain 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022954968.1	4.14e-169	60.13	tryptophan synthase beta chain 1-like [Cucurbita moschata]	[more]
KAG6573205.1	5.88e-169	60.35	Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_022954970.1	1.44e-165	60.35	tryptophan synthase beta chain 1-like [Cucurbita moschata]	[more]
XP_022994295.1	2.37e-164	59.91	tryptophan synthase beta chain 1-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG09g09050 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSL9	2.01e-169	60.13	Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1	[more]
A0A6J1GTV6	6.98e-166	60.35	Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457069 PE=3 SV=1	[more]
A0A6J1K0T4	1.15e-164	59.91	Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1	[more]
A0A0A0LUA1	8.85e-123	49.78	Tryptophan synthase OS=Cucumis sativus OX=3659 GN=Csa_1G064170 PE=3 SV=1	[more]
A0A1S3B3A1	2.67e-119	49.24	Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1	[more]

BLAST of Cp4.1LG09g09050 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G28237.1	1.5e-68	42.30	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]
AT5G54810.1	3.2e-66	37.79	tryptophan synthase beta-subunit 1	[more]
AT4G27070.1	2.1e-65	37.53	tryptophan synthase beta-subunit 2	[more]
AT5G38530.1	2.4e-05	24.46	tryptophan synthase beta type 2	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 171..191 e-value: 1.5E-36 score: 128.1
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 192..265 e-value: 1.5E-36 score: 128.1
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 80..169 e-value: 5.6E-29 score: 103.1
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 77..290
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 111..171 e-value: 1.4E-12 score: 47.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..75
None	No IPR available	PANTHER	PTHR48077	TRYPTOPHAN SYNTHASE-RELATED	coord: 102..170 coord: 264..294
None	No IPR available	PANTHER	PTHR48077	TRYPTOPHAN SYNTHASE-RELATED	coord: 64..102 coord: 170..263
None	No IPR available	PANTHER	PTHR48077:SF4	TRYPTOPHAN SYNTHASE	coord: 64..102 coord: 170..263
None	No IPR available	PANTHER	PTHR48077:SF4	TRYPTOPHAN SYNTHASE	coord: 102..170 coord: 264..294
IPR006653	Tryptophan synthase, beta chain, conserved site	PROSITE	PS00168	TRP_SYNTHASE_BETA	coord: 121..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g09050.1	Cp4.1LG09g09050.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006568	tryptophan metabolic process
molecular_function	GO:0004834	tryptophan synthase activity