Cp4.1LG09g07620 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g07620
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	gibberellin 2-beta-dioxygenase-like
Location	Cp4.1LG09: 7059885 .. 7061618 (-)
RNA-Seq Expression	Cp4.1LG09g07620
Synteny	Cp4.1LG09g07620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGTTCAATCACAGCTTGTGTTCGACCATTTTGCTCCGATCAAATCCTGCAAACACACCGCCCTGTTCACCGGAATTCCGGTGATCGACCTTTCCGACCCAGAAGCCAAAACCCATATCGTCAAAGCCTGTGAAGATTTCGGATTCTTTAAGGTTGTCAACCATGGCGTCTCCGACGACCTCATCGGAGCTCTCGAAGCCCAAGCTTTAAAATTCTTCCACCTCCCTCAATCTGAGAAGGAAAAAGCAGGGCCAGCTGACCCATTTGGATATGGCAGCAAGAGAATTGGCCCAAACGGCGATGTGGGTTGGATCGAATATCTCCTCCTAAACACGAATCATGAACTCATTTCTGAAAAATCCCTCTTCATTCTCCGTGAAAATCCAGAGATTTTCAGGTAAAAACACGTAAAATTACGAAGAACAGATTGCTCTGTTTTTTGGTTGCACTGTTTTTTGTTTGCTCTGTTTTTTGGTTGCTCTGTTTTCTAATTTGTCGTGTTTTTTGTCGTAGGTGTGCGGTAGAGGAGTATATATTGGCAGTGAAAAAGATGGCTTGTGAAGTGCTGGAAGCAATGGCGGAAGGGCTGGGGATAGAGAAACATGATTTGAGCAGGCTGTTGAAGGACGAGAGAAGTGATTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATTGGGTTTGGGGAACATACAGACCCACAAATAATATCTGTTCTGAGATCCAATAACACGACCGGACTCCATATTTGTTTGAGAGATGGAAGCTGGGTTCCAGTCCCACCTGATCAGAGGTCCTTTTTCATCAATGTTGGCGACTCATTGCAGGTATAGCCCACTCTTTCTCTGCCATTTTCATCTCAAGAAATGGCAATGGAATCTTGGGATGGTTTTGTTTATATTTTTGTTTCAAAGCCTACCCTTTTCTTAGGATTCTTGTGTCATCCTAACGAAGCATGCTTTATAAACATGTGGAAACCTCTCACACTAGTCGACCGTTTAAAAATCTTGAGGAAAGCTCAAAAAGACACTATTTACTAGTAGTGGACTTGAGCTGTTACTAATGGTATCAGAGCCAAACATCGGACAATGTGCTGAGGGGGTGGGCACGAGGAAATGTGCAAGCAAGGACCAAACACAGGGCGATGTGCCAGTGAAGAAGCTGGGCCCCAAAAGGGAGTGGACAGTGAGATCCCACATCAGTTGAGGAGGAGAACGAGGAGGCTGAGTCTCAAAGCGGGGTGGACACAAGGCGGTGTGCCAACAAGGACCAACACACCAGGTGATGTGCCAGCAAAGAGGTTGAGCCTCAAAGGGGTAGACACGAGGCGGTGTGCCAACAAGGACACTGGATCACGAGGCGGTGGCTTAGGCTGTTACGATTCTTGTTCTTATTGTTCTCGAGTGAATCATAATTCGGGTTGTATTGGTGCAGGTTATGACTAATGGGAGGTTCAACAGTGTGAAGCACAAGGTTTTAGCTGACCCAACAAAACCAAGGTTTTCAATGATATACTTTGGTGGGCCACCATTGAATGAAAAGATTGCACCTTTAGCATCACTATTGGAGGAGGGAGAGCAAAGGTTATACAAAGAGTTCACATGGTGGGATTACAAAAACTCAGCATATAAATCAAGGCTGGCTGCTTGTAGATTACGCCCTTTTGAGCTCAAAAATCTCACCCCTTTGTTGACTTGA

mRNA sequence

ATGGTGGTTCAATCACAGCTTGTGTTCGACCATTTTGCTCCGATCAAATCCTGCAAACACACCGCCCTGTTCACCGGAATTCCGGTGATCGACCTTTCCGACCCAGAAGCCAAAACCCATATCGTCAAAGCCTGTGAAGATTTCGGATTCTTTAAGGTTGTCAACCATGGCGTCTCCGACGACCTCATCGGAGCTCTCGAAGCCCAAGCTTTAAAATTCTTCCACCTCCCTCAATCTGAGAAGGAAAAAGCAGGGCCAGCTGACCCATTTGGATATGGCAGCAAGAGAATTGGCCCAAACGGCGATGTGGGTTGGATCGAATATCTCCTCCTAAACACGAATCATGAACTCATTTCTGAAAAATCCCTCTTCATTCTCCGTGAAAATCCAGAGATTTTCAGGTGTGCGGTAGAGGAGTATATATTGGCAGTGAAAAAGATGGCTTGTGAAGTGCTGGAAGCAATGGCGGAAGGGCTGGGGATAGAGAAACATGATTTGAGCAGGCTGTTGAAGGACGAGAGAAGTGATTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATTGGGTTTGGGGAACATACAGACCCACAAATAATATCTGTTCTGAGATCCAATAACACGACCGGACTCCATATTTGTTTGAGAGATGGAAGCTGGGTTCCAGTCCCACCTGATCAGAGGTCCTTTTTCATCAATGTTGGCGACTCATTGCAGGTTATGACTAATGGGAGGTTCAACAGTGTGAAGCACAAGGTTTTAGCTGACCCAACAAAACCAAGGTTTTCAATGATATACTTTGGTGGGCCACCATTGAATGAAAAGATTGCACCTTTAGCATCACTATTGGAGGAGGGAGAGCAAAGGTTATACAAAGAGTTCACATGGTGGGATTACAAAAACTCAGCATATAAATCAAGGCTGGCTGCTTGTAGATTACGCCCTTTTGAGCTCAAAAATCTCACCCCTTTGTTGACTTGA

Coding sequence (CDS)

ATGGTGGTTCAATCACAGCTTGTGTTCGACCATTTTGCTCCGATCAAATCCTGCAAACACACCGCCCTGTTCACCGGAATTCCGGTGATCGACCTTTCCGACCCAGAAGCCAAAACCCATATCGTCAAAGCCTGTGAAGATTTCGGATTCTTTAAGGTTGTCAACCATGGCGTCTCCGACGACCTCATCGGAGCTCTCGAAGCCCAAGCTTTAAAATTCTTCCACCTCCCTCAATCTGAGAAGGAAAAAGCAGGGCCAGCTGACCCATTTGGATATGGCAGCAAGAGAATTGGCCCAAACGGCGATGTGGGTTGGATCGAATATCTCCTCCTAAACACGAATCATGAACTCATTTCTGAAAAATCCCTCTTCATTCTCCGTGAAAATCCAGAGATTTTCAGGTGTGCGGTAGAGGAGTATATATTGGCAGTGAAAAAGATGGCTTGTGAAGTGCTGGAAGCAATGGCGGAAGGGCTGGGGATAGAGAAACATGATTTGAGCAGGCTGTTGAAGGACGAGAGAAGTGATTGTTGTTTCAGGCTGAACCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGGAATTTGATTGGGTTTGGGGAACATACAGACCCACAAATAATATCTGTTCTGAGATCCAATAACACGACCGGACTCCATATTTGTTTGAGAGATGGAAGCTGGGTTCCAGTCCCACCTGATCAGAGGTCCTTTTTCATCAATGTTGGCGACTCATTGCAGGTTATGACTAATGGGAGGTTCAACAGTGTGAAGCACAAGGTTTTAGCTGACCCAACAAAACCAAGGTTTTCAATGATATACTTTGGTGGGCCACCATTGAATGAAAAGATTGCACCTTTAGCATCACTATTGGAGGAGGGAGAGCAAAGGTTATACAAAGAGTTCACATGGTGGGATTACAAAAACTCAGCATATAAATCAAGGCTGGCTGCTTGTAGATTACGCCCTTTTGAGCTCAAAAATCTCACCCCTTTGTTGACTTGA

Protein sequence

MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSFFINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRLYKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT

Homology

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BLAST of Cp4.1LG09g07620 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 5.3e-131
Identity = 229/328 (69.82%), Postives = 265/328 (80.79%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVV SQ   + F  +K  K T LFTGIPV+DL+ P+AK  IV AC DFGFFK+VNHGV  
Sbjct: 1   MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +L+  LE +AL+FF   QSEK++AGP DPFGYGSKRIGPNGDVGW+EYLLLNTN ++IS 
Sbjct: 61  ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGI-EKHDLSRLLKDERSDCCF 180
           KSL I RENP  FR  VE YI AVK M   VLE MAEGLGI +++ LSRLLKDE+SD CF
Sbjct: 121 KSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCF 180

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRS 240
           RLNHYPPCPE++AL+ RNL+GFGEHTDPQIISVLRSN+T+GL ICL DG+WV VPPDQ S
Sbjct: 181 RLNHYPPCPEVQALN-RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTS 240

Query: 241 FFINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQR 300
           FFINVGD+LQVMTNGRF SVKH+VLAD TK R SMIYFGGP L+E IAPL S++ +GE+ 
Sbjct: 241 FFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEEC 300

Query: 301 LYKEFTWWDYKNSAYKSRLAACRLRPFE 328
           LYKEFTW +YK +AY SRLA  RL PF+
Sbjct: 301 LYKEFTWCEYKKAAYTSRLADNRLAPFQ 327

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.8e-113
Identity = 199/328 (60.67%), Postives = 254/328 (77.44%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MV+ S+   + +  +++       + IP++DLS P+AKT IVKACEDFGFFKV+NHG+  
Sbjct: 1   MVLLSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPL 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           D I  LE++A KFF LPQ+EKEKAGPA+PFGYG+KRIG NGD+GWIEYLLL TN     +
Sbjct: 61  DAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTN----QD 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIE-KHDLSRLLKDERSDCCF 180
            +  +  E+ + FR  +++Y  A++ MACE+L+ MAEGL I+ K+  S+L+ D++SDC F
Sbjct: 121 HNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLF 180

Query: 181 RLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRS 240
           R+NHYP CPEL A++G NLIGFGEHTDPQIIS+LRSNNT+G  I LRDGSW+ VPPD  S
Sbjct: 181 RVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSS 240

Query: 241 FFINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQR 300
           FFINVGDSLQVMTNGRF SV+H+VLA+   PR SMIYF GPPL+EKIAPL SL+ +G++ 
Sbjct: 241 FFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKES 300

Query: 301 LYKEFTWWDYKNSAYKSRLAACRLRPFE 328
           LYKEFTW++YK+S Y SRLA  RL  +E
Sbjct: 301 LYKEFTWFEYKSSTYGSRLADNRLGNYE 322

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 4.9e-113
Identity = 206/336 (61.31%), Postives = 253/336 (75.30%), Query Frame = 0


Query: 1   MVVQSQLVF--DHFAPIKSCKHTALFT--GIPVIDLSDPEAKTHIVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   + T   IPV++L+DPEAKT IVKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDDLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHE 120
           GV  +L+  LE +A+ FF LPQS K +AGP +P+GYG+KRIGPNGDVGWIEYLLLN N +
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISEKSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHD-LSRLLKDERS 180
           L S K+  + R+ P+IFR +VEEY+  +K+++ +VLE +AE LGIE  D LS++L+DE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPP 240
           D C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DGSWV VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQRSFFINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEE 300
           D  SFFINVGD+LQVMTNGRF SVKH+VLAD  + R SMIYFGGPPL++KIAPL  L+ E
Sbjct: 241 DHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPE 300

Query: 301 GEQRLYKEFTWWDYKNSAYKSRLAACRLRPFELKNL 332
            +  LYKEFTW  YK+SAYKS+L   RL  FE + L
Sbjct: 301 QDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEKQPL 334

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 381.3 bits (978), Expect = 1.1e-104
Identity = 185/315 (58.73%), Postives = 235/315 (74.60%), Query Frame = 0


Query: 24  FTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGALEAQALKFFHLPQSEKEK 83
           F+ IPVID+SDPE+K  +VKACEDFGFFKV+NHGVS +L+  LE + + FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 84  AGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILA 143
                PFGYG+ +IG NGDVGW+EYLL+N NH+  S      L ++P  FR A+EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 144 VKKMACEVLEAMAEGLGIE-KHDLSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 203
           V+KM  +VLE + +GLGI+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 204 FGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSFFINVGDSLQVMTNGRFNSVK 263
           FGEHTDPQIISVLRSNNT+GL I L DGSW+ VPPD  SFF NVGDSLQVMTNGRF SV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 264 HKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRLYKEFTWWDYKNSAYKSRLAA 323
           H+VLA+  K R SMIYF GP L ++IAPL  L++  ++RLY+EFTW +YKNS Y SRL+ 
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 324 CRLRPFELKNLTPLL 336
            RL+ FE K +  LL
Sbjct: 315 NRLQQFERKTIKNLL 328

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.5e-101
Identity = 183/302 (60.60%), Postives = 224/302 (74.17%), Query Frame = 0


Query: 27  IPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGALEAQALKFFHLPQSEKEKAGP 86
           IPVIDL+D +AKT IVKACE+FGFFKV+NHGV  DL+  LE +A+ FF L  S K+KAGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 87  ADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKK 146
            DPFGYG+KRIGPNGD+GW+EY+LLN N  L S K+  I R  P IFR AVEEYI  +K+
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 147 MACEVLEAMAEGLGIE-KHDLSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHT 206
           M+ + LE + E L IE K  LSRL+K + SD C R+NHY   PE E    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 207 DPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSFFINVGDSLQVMTNGRFNSVKHKVLA 266
           DPQ+IS+LRSN+T GL IC++DG+WV V PD  SFF+ VGD+LQVMTNGRF SVKH+V+ 
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 267 DPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRLYKEFTWWDYKNSAYKSRLAACRLRP 326
           +  + R SMIYF GPPL+EKIAPL+ L+ + +  LY EFTW  YK SAYK++L   RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 325

Query: 327 FE 328
           FE
Sbjct: 327 FE 325

[Close]

BLAST of Cp4.1LG09g07620 vs. NCBI nr
Match: XP_023542402.1 (gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 695 bits (1794), Expect = 7.90e-253
Identity = 336/336 (100.00%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. NCBI nr
Match: XP_022994838.1 (gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 694 bits (1792), Expect = 1.59e-252
Identity = 335/336 (99.70%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLAS+LEEGEQRL
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASILEEGEQRL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. NCBI nr
Match: XP_022955233.1 (gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 689 bits (1778), Expect = 2.17e-250
Identity = 333/336 (99.11%), Postives = 335/336 (99.70%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVS+
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSN 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  ELIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLL EGEQRL
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLVEGEQRL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. NCBI nr
Match: KAG6573363.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 686 bits (1770), Expect = 8.55e-249
Identity = 334/341 (97.95%), Postives = 336/341 (98.53%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 19  MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 78

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 79  ELIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 138

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 139 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 198

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 199 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 258

Query: 241 FINVGDSLQV-----MTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEE 300
           FINVGDSLQV     MTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEE
Sbjct: 259 FINVGDSLQVVLVQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEE 318

Query: 301 GEQRLYKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           GE+RLYKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 319 GEKRLYKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 359

[Close]

BLAST of Cp4.1LG09g07620 vs. NCBI nr
Match: KAG7012527.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 664 bits (1712), Expect = 1.66e-240
Identity = 324/336 (96.43%), Postives = 325/336 (96.73%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  ELIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQ           HKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL
Sbjct: 241 FINVGDSLQ-----------HKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 325

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy TrEMBL
Match: A0A6J1K680 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490445 PE=3 SV=1)

HSP 1 Score: 694 bits (1792), Expect = 7.72e-253
Identity = 335/336 (99.70%), Postives = 336/336 (100.00%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLAS+LEEGEQRL
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASILEEGEQRL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy TrEMBL
Match: A0A6J1GTD6 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457257 PE=3 SV=1)

HSP 1 Score: 689 bits (1778), Expect = 1.05e-250
Identity = 333/336 (99.11%), Postives = 335/336 (99.70%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVS+
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSN 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE
Sbjct: 61  ELIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLL EGEQRL
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLVEGEQRL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy TrEMBL
Match: I0J1I9 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1)

HSP 1 Score: 624 bits (1608), Expect = 8.63e-225
Identity = 303/336 (90.18%), Postives = 311/336 (92.56%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCK TALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIG LEAQALKFF LPQSEKEKAGP DPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPEIFRCAVEEYILAVK+MACEVLEAMA GL IEK  LSRLLKDERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDG+WV VPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRF SVKHKVLADP K R SMIYFGGPPLNEKIAPL SL+EEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           Y+EFTWWDYK SAYKSRLA CRL PFE+K LTPLLT
Sbjct: 301 YREFTWWDYKTSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy TrEMBL
Match: A0A6J1EMS7 (gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC111433993 PE=3 SV=1)

HSP 1 Score: 622 bits (1605), Expect = 2.47e-224
Identity = 302/336 (89.88%), Postives = 311/336 (92.56%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCK TALFTGIPVIDL DPEAKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
            LIG LEAQALKFF LPQSEKEKAGP DPFGYGSKRIGPNGDVGWIEY+LLNTNHELISE
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KSLFILRENPE+FRCAVEEYILAVK+MACEVLEAMA GL IEK  LS LLKDERSDCCFR
Sbjct: 121 KSLFILRENPEVFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSWLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDG+WV VPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRF SVKHKVLADP K R SMIYFGGPPLNEKIAPL SL+EEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           Y+EFTWWDYKNSAYKSRLA CRL PFE+K LTPLLT
Sbjct: 301 YREFTWWDYKNSAYKSRLADCRLGPFEIKTLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. ExPASy TrEMBL
Match: S0BE08 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1)

HSP 1 Score: 609 bits (1570), Expect = 5.33e-219
Identity = 293/336 (87.20%), Postives = 309/336 (91.96%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPIKSCK TALFTGIPVI+L DPEAK  IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKIQIVKACEDFGFFKLVNHGVSD 60

Query: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120
           +LI ALE+Q LKFFHLPQSEKEKAGP DPFGYGSKRIGPNGDVGWIEYLLLNTN +LIS+
Sbjct: 61  ELIAALESQTLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120

Query: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180
           KS+ I  ENPEIFRC VEEYI A+K+MAC+VLE MAEGL IEK  LS+LLKDERSDCCFR
Sbjct: 121 KSISIFHENPEIFRCLVEEYISAMKEMACQVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240
           LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WV VPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300
           FINVGDSLQVMTNGRF SVKHKVLADP K RFSMIYFGGPPLNEKIAPL SLLEEGE+ L
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEKSL 300

Query: 301 YKEFTWWDYKNSAYKSRLAACRLRPFELKNLTPLLT 336
           Y+EFTWWDYK+SAYKSRLA CRL PFE+KNLTPLLT
Sbjct: 301 YREFTWWDYKSSAYKSRLADCRLTPFEIKNLTPLLT 336

[Close]

BLAST of Cp4.1LG09g07620 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 409.1 bits (1050), Expect = 3.5e-114
Identity = 206/336 (61.31%), Postives = 253/336 (75.30%), Query Frame = 0


Query: 1   MVVQSQLVF--DHFAPIKSCKHTALFT--GIPVIDLSDPEAKTHIVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   + T   IPV++L+DPEAKT IVKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDDLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHE 120
           GV  +L+  LE +A+ FF LPQS K +AGP +P+GYG+KRIGPNGDVGWIEYLLLN N +
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISEKSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHD-LSRLLKDERS 180
           L S K+  + R+ P+IFR +VEEY+  +K+++ +VLE +AE LGIE  D LS++L+DE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPP 240
           D C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DGSWV VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQRSFFINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEE 300
           D  SFFINVGD+LQVMTNGRF SVKH+VLAD  + R SMIYFGGPPL++KIAPL  L+ E
Sbjct: 241 DHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPE 300

Query: 301 GEQRLYKEFTWWDYKNSAYKSRLAACRLRPFELKNL 332
            +  LYKEFTW  YK+SAYKS+L   RL  FE + L
Sbjct: 301 QDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEKQPL 334

[Close]

BLAST of Cp4.1LG09g07620 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 381.3 bits (978), Expect = 7.8e-106
Identity = 185/315 (58.73%), Postives = 235/315 (74.60%), Query Frame = 0


Query: 24  FTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGALEAQALKFFHLPQSEKEK 83
           F+ IPVID+SDPE+K  +VKACEDFGFFKV+NHGVS +L+  LE + + FF LP+SEK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 84  AGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILA 143
                PFGYG+ +IG NGDVGW+EYLL+N NH+  S      L ++P  FR A+EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 144 VKKMACEVLEAMAEGLGIE-KHDLSRLLKDERSDCCFRLNHYPPCP--ELEALSGRNLIG 203
           V+KM  +VLE + +GLGI+ ++ LS+L+ D+ +D   RLNHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 204 FGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSFFINVGDSLQVMTNGRFNSVK 263
           FGEHTDPQIISVLRSNNT+GL I L DGSW+ VPPD  SFF NVGDSLQVMTNGRF SV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 264 HKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRLYKEFTWWDYKNSAYKSRLAA 323
           H+VLA+  K R SMIYF GP L ++IAPL  L++  ++RLY+EFTW +YKNS Y SRL+ 
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

Query: 324 CRLRPFELKNLTPLL 336
            RL+ FE K +  LL
Sbjct: 315 NRLQQFERKTIKNLL 328

[Close]

BLAST of Cp4.1LG09g07620 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-102
Identity = 183/302 (60.60%), Postives = 224/302 (74.17%), Query Frame = 0


Query: 27  IPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSDDLIGALEAQALKFFHLPQSEKEKAGP 86
           IPVIDL+D +AKT IVKACE+FGFFKV+NHGV  DL+  LE +A+ FF L  S K+KAGP
Sbjct: 27  IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALHHSLKDKAGP 86

Query: 87  ADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAVKK 146
            DPFGYG+KRIGPNGD+GW+EY+LLN N  L S K+  I R  P IFR AVEEYI  +K+
Sbjct: 87  PDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAVEEYIKEMKR 146

Query: 147 MACEVLEAMAEGLGIE-KHDLSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGEHT 206
           M+ + LE + E L IE K  LSRL+K + SD C R+NHY   PE E    +  IGFGEHT
Sbjct: 147 MSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVKEEIGFGEHT 206

Query: 207 DPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSFFINVGDSLQVMTNGRFNSVKHKVLA 266
           DPQ+IS+LRSN+T GL IC++DG+WV V PD  SFF+ VGD+LQVMTNGRF SVKH+V+ 
Sbjct: 207 DPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRFKSVKHRVVT 266

Query: 267 DPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRLYKEFTWWDYKNSAYKSRLAACRLRP 326
           +  + R SMIYF GPPL+EKIAPL+ L+ + +  LY EFTW  YK SAYK++L   RL  
Sbjct: 267 NTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKTKLGDYRLGL 325

Query: 327 FE 328
           FE
Sbjct: 327 FE 325

[Close]

BLAST of Cp4.1LG09g07620 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 307.0 bits (785), Expect = 1.9e-83
Identity = 153/305 (50.16%), Postives = 199/305 (65.25%), Query Frame = 0


Query: 28  PVIDLS---DPEAKTHIVKACEDFGFFKVVNHGVSDDLIGALEAQALKFFHLPQSEKEKA 87
           PVID S     +    IVKACE  GFFKV+NHGV  ++I   E +  +FF+ P+S+K +A
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 88  GPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISEKSLFILRENPEIFRCAVEEYILAV 147
           GPA PFGYG K IG NGD+G +EYLLL+ N   +++KS  I  ++P  F  A  +YI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 148 KKMACEVLEAMAEGL-GIEKHDLSRLLKDERSDCCFRLNHYPPCPELEALSGRNLIGFGE 207
           + +ACE+++   E L G +  ++S L++D RSD   RLNHYPP P   ALSG   IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 208 HTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSFFINVGDSLQVMTNGRFNSVKHKV 267
           H+DPQI++VLRSN+  GL IC RDG W+P+P D   FF+ VGD LQ +TNGRF SV+H+V
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRV 264

Query: 268 LADPT-KPRFSMIYFGGPPLNEKIAPLASLLEEGEQRLYKEFTWWDYKNSAYKSRLAACR 327
           LA+   KPR S +YF  PPL  KI+PL  ++     R Y  FTW DYK + Y  RL   R
Sbjct: 265 LANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRLDVPR 324

[Close]

BLAST of Cp4.1LG09g07620 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 306.2 bits (783), Expect = 3.2e-83
Identity = 154/255 (60.39%), Postives = 192/255 (75.29%), Query Frame = 0


Query: 1   MVVQSQLVF--DHFAPIKSCKHTALFT--GIPVIDLSDPEAKTHIVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   + T   IPV++L+DPEAKT IVKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDDLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHE 120
           GV  +L+  LE +A+ FF LPQS K +AGP +P+GYG+KRIGPNGDVGWIEYLLLN N +
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISEKSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHD-LSRLLKDERS 180
           L S K+  + R+ P+IFR +VEEY+  +K+++ +VLE +AE LGIE  D LS++L+DE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRLNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPP 240
           D C RLNHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DGSWV VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQRSFFINVGDSLQV 251
           D  SFFINVGD+LQV
Sbjct: 241 DHSSFFINVGDALQV 253

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g07620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	5.3e-131	69.82	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9SQ80	3.8e-113	60.67	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XFR9	4.9e-113	61.31	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q8LEA2	1.1e-104	58.73	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]
O64692	2.5e-101	60.60	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]

BLAST of Cp4.1LG09g07620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023542402.1	7.90e-253	100.00	gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022994838.1	1.59e-252	99.70	gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita maxima]	[more]
XP_022955233.1	2.17e-250	99.11	gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita moschata]	[more]
KAG6573363.1	8.55e-249	97.95	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7012527.1	1.66e-240	96.43	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of Cp4.1LG09g07620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K680	7.72e-253	99.70	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A6J1GTD6	1.05e-250	99.11	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
I0J1I9	8.63e-225	90.18	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1	[more]
A0A6J1EMS7	2.47e-224	89.88	gibberellin 2-beta-dioxygenase-like OS=Cucurbita moschata OX=3662 GN=LOC11143399...	[more]
S0BE08	5.33e-219	87.20	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1	[more]

BLAST of Cp4.1LG09g07620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	3.5e-114	61.31	gibberellin 2-oxidase	[more]
AT1G78440.1	7.8e-106	58.73	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT2G34555.1	1.8e-102	60.60	gibberellin 2-oxidase 3	[more]
AT1G02400.1	1.9e-83	50.16	gibberellin 2-oxidase 6	[more]
AT1G30040.2	3.2e-83	60.39	gibberellin 2-oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 204..225 score: 27.69 coord: 240..266 score: 28.96 coord: 41..58 score: 56.06
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 7..330
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 7..330
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 13..317
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..278 e-value: 2.8E-25 score: 88.7
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 27..97 e-value: 1.5E-16 score: 61.2
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..319 e-value: 2.9E-82 score: 278.6
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 174..280 score: 11.273666

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g07620.1	Cp4.1LG09g07620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding