Cp4.1LG09g07280 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g07280 vs. ExPASy Swiss-Prot
Match: Q2UVJ5 (Cation-chloride cotransporter 1 OS=Arabidopsis thaliana OX=3702 GN=CCC1 PE=1 SV=1)

HSP 1 Score: 1556.6 bits (4029), Expect = 0.0e+00
Identity = 793/1010 (78.51%), Postives = 879/1010 (87.03%), Query Frame = 0

Query: 77   MDNADIE----SGEEDFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHP 136
            MD+ DIE    +GEE+F  G R  G KYRPV AHDRAV+EMSS+DPG +SST        
Sbjct: 1    MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSST-------- 60

Query: 137  SMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFD 196
                          L     V    VG+  G  G E        +NG Q ESKLE FGFD
Sbjct: 61   --------------LKNIKVVAPGDVGA--GVRGPEDG------VNGHQKESKLELFGFD 120

Query: 197  SLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGII 256
            SLVNILGLKSM GEQIQAPSSPRDGE++SI+QG PK   +K GT+MGVF+PCLQNILGII
Sbjct: 121  SLVNILGLKSMTGEQIQAPSSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGII 180

Query: 257  YYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPE 316
            YYIRF+WIVGMAGIG+ LVLV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPE
Sbjct: 181  YYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPE 240

Query: 317  VGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTV-EPIQSPSSH 376
            VG+SIGLCFFLGNAVAGALYVLGAVETFL A P+AGIFRET+TKVNG+ V E IQSP+SH
Sbjct: 241  VGISIGLCFFLGNAVAGALYVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSH 300

Query: 377  DLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTG 436
            DLQVYGI+VTI LCFIVFGGVKMINRVAPAFL+PVL S+ CIF+GIFLAK +DP +G+TG
Sbjct: 301  DLQVYGIVVTILLCFIVFGGVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITG 360

Query: 437  LSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDT 496
            L + +FK+NW S YQMTN AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDT
Sbjct: 361  LRLKSFKDNWGSAYQMTNDAGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDT 420

Query: 497  QRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILS 556
            Q+SIP+GTL ATL TT LYL+SVL FGA+ATR +LLTDRL TAT+AWPFPAI++VGIILS
Sbjct: 421  QKSIPVGTLAATLTTTSLYLISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILS 480

Query: 557  TLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLD 616
            TLGAALQSLTGAPRLLAAIAND+ILPILNYFKV D  EP+ ATLFTA +CIGCV+IGNLD
Sbjct: 481  TLGAALQSLTGAPRLLAAIANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLD 540

Query: 617  LITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISW 676
            LITPTVTMF+LLCY+GVNLSCFLLDLLDAPSWRPRWK+HHWSLS +GASLC+VIMFLISW
Sbjct: 541  LITPTVTMFYLLCYSGVNLSCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISW 600

Query: 677  SFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPL 736
            SFT+V++ALASLIY YV L+GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL
Sbjct: 601  SFTVVAIALASLIYKYVGLKGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPL 660

Query: 737  IFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLAT 796
            +FCRPWG+LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDY+E  E++K ACKQLAT
Sbjct: 661  VFCRPWGQLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLAT 720

Query: 797  YIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATF 856
            YI+YKRCEGVAEIVVAP+M+EGFRGI+QTMGLGNLKPNI+VMRYPEIWRRENLTEIP+TF
Sbjct: 721  YIEYKRCEGVAEIVVAPNMTEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTF 780

Query: 857  VGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 916
            VGIINDCI ANKAVVI+KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE
Sbjct: 781  VGIINDCITANKAVVIIKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 840

Query: 917  SCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEA 976
            SCKIQ+FCIAEEDSDAEALKADVKKFLYDLRM AEVIV+T+KSWD  + EG  Q++S+EA
Sbjct: 841  SCKIQLFCIAEEDSDAEALKADVKKFLYDLRMHAEVIVVTMKSWD-IRSEGNSQEDSLEA 900

Query: 977  FTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSR 1036
            F A Q RI+ YLGE+K       +  L+A+GKP+ VNE+QV+KFL T LKLNS+IL YSR
Sbjct: 901  FDAAQRRISDYLGEIK----RQGSNPLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSR 960

Query: 1037 MAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1079
            MAAVVLVSLPPPP NHPAYFYMEY+DLLVENVPR+LIVRGY RDVVTL+T
Sbjct: 961  MAAVVLVSLPPPPLNHPAYFYMEYMDLLVENVPRMLIVRGYHRDVVTLFT 975

BLAST of Cp4.1LG09g07280 vs. ExPASy Swiss-Prot
Match: Q6Z0E2 (Cation-chloride cotransporter 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CCC1 PE=2 SV=1)

HSP 1 Score: 1513.4 bits (3917), Expect = 0.0e+00
Identity = 770/1022 (75.34%), Postives = 865/1022 (84.64%), Query Frame = 0

Query: 77   MDNADIESGEEDF----HGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHPSMK 136
            M+N +IE   +D         GR+YRPV + DRAV++M+SM+PG +SST           
Sbjct: 1    MENGEIEGAADDGVPVPAPPNGRRYRPVGSSDRAVIQMTSMEPGSSSST----------- 60

Query: 137  VNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTL-----VEINGPQGESKLEFFG 196
                                  V + +G   +   +LT+      +    QG+SKLE FG
Sbjct: 61   ---------------------AVAAVSGITPQPPRNLTVDPSMQEDHTVSQGDSKLELFG 120

Query: 197  FDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILG 256
            FDSLVNILGLKSM GEQIQAPSSPRDGE+V+I+ G PK    K GT+MGVF+PCLQNILG
Sbjct: 121  FDSLVNILGLKSMTGEQIQAPSSPRDGEDVAITIGRPKETGPKFGTMMGVFVPCLQNILG 180

Query: 257  IIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALG 316
            IIYYIRF+WIVGMAG+ +SLVLV+FCG CTFLT ISLSAIATNGAMKGGGPYYLIGRALG
Sbjct: 181  IIYYIRFTWIVGMAGVWQSLVLVSFCGACTFLTGISLSAIATNGAMKGGGPYYLIGRALG 240

Query: 317  PEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE------- 376
            PEVGVSIGLCFFLGNAVAG++YVLGAVETFLDAVPSAG F+E+VT VN + V        
Sbjct: 241  PEVGVSIGLCFFLGNAVAGSMYVLGAVETFLDAVPSAGFFKESVTVVNNTLVNGTATAST 300

Query: 377  -PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKK 436
              I +PS HDLQVYG+IVTI LCFIVFGGVK+IN+VAPAFLIPVLFS++CI+LG+F+A +
Sbjct: 301  ATISTPSLHDLQVYGVIVTILLCFIVFGGVKIINKVAPAFLIPVLFSLLCIYLGVFIAPR 360

Query: 437  NDPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGS 496
            ++   G+TGLS+ TFK+NW S+YQ TN+AG+PDP G++YW+FNALVGLFFPAVTGIMAGS
Sbjct: 361  HNAPKGITGLSITTFKDNWGSEYQRTNNAGVPDPNGSIYWDFNALVGLFFPAVTGIMAGS 420

Query: 497  NRSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPA 556
            NRSASLKDTQRSIP+GTL ATL TT +YL SVLLFGALATR+ LLTDRL TATVAWP PA
Sbjct: 421  NRSASLKDTQRSIPIGTLSATLTTTAMYLFSVLLFGALATREELLTDRLLTATVAWPAPA 480

Query: 557  IIYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCI 616
            +IY+GIILSTLGAALQSLTGAPRLLAAIAND+ILP+LNYFKV +G EP+ ATLFTA +CI
Sbjct: 481  VIYIGIILSTLGAALQSLTGAPRLLAAIANDDILPVLNYFKVSEGAEPHSATLFTAFICI 540

Query: 617  GCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLC 676
             CV+IGNLDLITPT+TMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSL+GA LC
Sbjct: 541  CCVVIGNLDLITPTITMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLVGALLC 600

Query: 677  VVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVH 736
            VVIMFLISWSFT+VSLALASLIYYYV L+GKAGDWGDGFKSAYFQLALRSLRSLGA+QVH
Sbjct: 601  VVIMFLISWSFTVVSLALASLIYYYVSLKGKAGDWGDGFKSAYFQLALRSLRSLGANQVH 660

Query: 737  PKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDS 796
            PKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSI+DGDYHE  ED+
Sbjct: 661  PKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSIIDGDYHELAEDA 720

Query: 797  KAACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRE 856
            K AC+QL TYI+YKRCEGVAEI+VAPSMSEGFR IVQTMGLGNLKPNIIVMRYPEIWRRE
Sbjct: 721  KTACRQLDTYIEYKRCEGVAEIIVAPSMSEGFRSIVQTMGLGNLKPNIIVMRYPEIWRRE 780

Query: 857  NLTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQ 916
            NL +IP+TFV IINDCI ANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQ
Sbjct: 781  NLIQIPSTFVSIINDCIIANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQ 840

Query: 917  LLLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWD---TTQ 976
            LLLTKE+FESCKIQVFCIAEED+DAE LKADVKKFLYDLRM AEVIV+T+KSW+    + 
Sbjct: 841  LLLTKETFESCKIQVFCIAEEDTDAEELKADVKKFLYDLRMHAEVIVVTMKSWEPHMESS 900

Query: 977  VEGGQQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTT 1036
              G  QD+S EA+T+ Q RI++YL EMK   A+     LM DGK V VNE++++KFL T 
Sbjct: 901  SSGAPQDDSQEAYTSAQRRISTYLSEMK-ETAQREGHPLMEDGKQVVVNEQKIEKFLYTM 960

Query: 1037 LKLNSSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTL 1079
             KLNS+ILRYSRMAAVVLVSLPPPP NHPAYFYMEY+DLLVENVPR+LIVRGYRRDVVT 
Sbjct: 961  FKLNSTILRYSRMAAVVLVSLPPPPLNHPAYFYMEYMDLLVENVPRMLIVRGYRRDVVTF 989

BLAST of Cp4.1LG09g07280 vs. ExPASy Swiss-Prot
Match: Q657W3 (Cation-chloride cotransporter 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CCC2 PE=2 SV=2)

HSP 1 Score: 1448.7 bits (3749), Expect = 0.0e+00
Identity = 731/994 (73.54%), Postives = 840/994 (84.51%), Query Frame = 0

Query: 95   RKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHPSMKVNFGFGFAALFLNRYHDVMKVK 154
            ++YR VE+HDRAV++M+ M+ G  SS  +  +  P                RY     +K
Sbjct: 25   QRYRTVESHDRAVVQMAPMEFG--SSADASASAGP----------------RY-----IK 84

Query: 155  VGSQTGTDGK-EGNSLTLVEINGPQGESKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRD 214
             G+   TD +    S      NG QG+SKLE FGFDSLVNILGLK M+GEQ QA +S RD
Sbjct: 85   PGTNLRTDARMHMASSNGRSSNGSQGDSKLELFGFDSLVNILGLKRMVGEQAQASASTRD 144

Query: 215  GENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGESLVLVAFC 274
            GEN  I+ G PK  E K  T+MGVF+PCLQNILGIIYYIRF+WIVGM G+ +SLVLVAFC
Sbjct: 145  GENAGIAIGHPKETETKLDTMMGVFVPCLQNILGIIYYIRFTWIVGMGGVWQSLVLVAFC 204

Query: 275  GLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGALYVLGA 334
            G CTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGA+YVLGA
Sbjct: 205  GSCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAGAMYVLGA 264

Query: 335  VETFLDAVPSAGIFRETVTKVNGSTVE--------PIQSPSSHDLQVYGIIVTIFLCFIV 394
            VETFLDAVPSA  F+E+VT V  + V          I +P+ HDLQVYGIIVTI LCFIV
Sbjct: 265  VETFLDAVPSAEFFQESVTVVTNTFVNGTAAGNATTISTPNLHDLQVYGIIVTILLCFIV 324

Query: 395  FGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFKENWSSDYQMT 454
            FGGVK+IN+VAPAFLIPVLFS++CI++G+F+A + + +  +TGLS+ T K+NWSSDYQ T
Sbjct: 325  FGGVKIINKVAPAFLIPVLFSILCIYIGVFIAPRPNASKWITGLSITTLKDNWSSDYQRT 384

Query: 455  NSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMGTLFATLLTTI 514
            N+AG+PDP G++YW+FNAL+GL+FPAVTGIMAGSNRSASLKDTQRSIP+GTL AT+ TT+
Sbjct: 385  NNAGVPDPNGSIYWDFNALLGLYFPAVTGIMAGSNRSASLKDTQRSIPIGTLHATISTTM 444

Query: 515  LYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQSLTGAPRLLA 574
            +YL+SV LFGAL+TR+ LLTDRL  A VAWP PA++Y GIILSTLGAALQSLTGAPRLLA
Sbjct: 445  MYLLSVFLFGALSTREGLLTDRLLCAAVAWPSPAVVYAGIILSTLGAALQSLTGAPRLLA 504

Query: 575  AIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVTMFFLLCYAGV 634
            AIAND+ILP+LNYFK  +G EP+ ATLFT+ +CI CVIIGNLD+ITPT+TMFFLLCYAGV
Sbjct: 505  AIANDDILPVLNYFKAYEGSEPHVATLFTSFICISCVIIGNLDVITPTITMFFLLCYAGV 564

Query: 635  NLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSLALASLIYYYV 694
            NLSCFLLDLLDAPSWRPRWK HHWSLSL+GA LC+VIMF+ISW+FT+VSLALASLIYYYV
Sbjct: 565  NLSCFLLDLLDAPSWRPRWKLHHWSLSLIGALLCIVIMFMISWTFTVVSLALASLIYYYV 624

Query: 695  CLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGKLPENVPCHP 754
             L+GKAGDWGDGFKSAYFQLALRSLRS+GA+QVHPKNWYPIPLIFCRPWGKLPE+VPCHP
Sbjct: 625  SLKGKAGDWGDGFKSAYFQLALRSLRSMGANQVHPKNWYPIPLIFCRPWGKLPEDVPCHP 684

Query: 755  KLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRCEGVAEIVVAP 814
            KLADFANCMKKKGRGMSIFVSI+DGDYHE  ED+K AC+QL+ YIDY+RCEGVAEI+VAP
Sbjct: 685  KLADFANCMKKKGRGMSIFVSIIDGDYHESAEDAKTACRQLSAYIDYRRCEGVAEIIVAP 744

Query: 815  SMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDCIDANKAVVIV 874
            S S GFR IVQTMGLGNLKPNI+VMRYPEIWRRENLT+IP+TFV IINDCI ANKAVVIV
Sbjct: 745  STSIGFRSIVQTMGLGNLKPNIVVMRYPEIWRRENLTQIPSTFVSIINDCITANKAVVIV 804

Query: 875  KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDSDAE 934
            KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEED++AE
Sbjct: 805  KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFCIAEEDTEAE 864

Query: 935  ALKADVKKFLYDLRMQAEVIVITIKSWDT-TQVEGGQQDESIEAFTAGQGRIASYLGEMK 994
             LKADVKKFLYDLRMQA+VIV+T+KSW+      GG + +  E + + Q RI +Y+ ++K
Sbjct: 865  ELKADVKKFLYDLRMQADVIVVTVKSWEADPDRSGGSKKDDPEVYRSAQSRIRTYISQLK 924

Query: 995  AAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLVSLPPPPPNH 1054
             AA   R   LM  GK V V+E++V+KFL T LKLN++ILR+SRMA VVLVSLPPPP NH
Sbjct: 925  EAAERER-RPLMEGGKQVVVDEQKVEKFLYTMLKLNATILRHSRMAVVVLVSLPPPPLNH 984

Query: 1055 PAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1079
             AY YMEY+DLLVEN+PR+LIVRGYRRDVVTL+T
Sbjct: 985  LAYCYMEYMDLLVENIPRILIVRGYRRDVVTLFT 994

BLAST of Cp4.1LG09g07280 vs. ExPASy Swiss-Prot
Match: Q9Y666 (Solute carrier family 12 member 7 OS=Homo sapiens OX=9606 GN=SLC12A7 PE=1 SV=3)

HSP 1 Score: 539.3 bits (1388), Expect = 1.0e-151
Identity = 335/1002 (33.43%), Postives = 534/1002 (53.29%), Query Frame = 0

Query: 218  SISQGLPKNEE-----------VKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGES 277
            ++SQG+ ++EE            + GT +GV++PCLQNILG+I ++R +WIVG+AG+ ES
Sbjct: 94   NLSQGVVEHEEDEESRRREAKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGVAGVLES 153

Query: 278  LVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAG 337
             ++VA C  CT LT+IS+SAIATNG +  GG YY+I R+LGPE G ++GLCF+LG   AG
Sbjct: 154  FLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAG 213

Query: 338  ALYVLGAVETFLDAV-PSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGIIVTIFLCFIV 397
            A+Y+LG +E FL  + P A IF+        + +        H+++VYG    + +  +V
Sbjct: 214  AMYILGTIEIFLTYISPGAAIFQAEAAGGEAAAM-------LHNMRVYGTCTLVLMALVV 273

Query: 398  FGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPAD--------------------- 457
            F GVK +N++A  FL  V+ S++ I+ G+ +    DP D                     
Sbjct: 274  FVGVKYVNKLALVFLACVVLSILAIYAGV-IKSAFDPPDIPVCLLGNRTLSRRSFDACVK 333

Query: 458  ----------------------------------------GVTGLSMDTFKENWSSDY-- 517
                                                    G+ G +   F EN  S Y  
Sbjct: 334  AYGIHNNSATSALWGLFCNGSQPSAACDEYFIQNNVTEIQGIPGAASGVFLENLWSTYAH 393

Query: 518  ----------------QMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLK 577
                            + + ++ +P    ++  +F  LVG++FP+VTGIMAGSNRS  LK
Sbjct: 394  AGAFVEKKGVPSVPVAEESRASALPYVLTDIAASFTLLVGIYFPSVTGIMAGSNRSGDLK 453

Query: 578  DTQRSIPMGTLFATLLTTILYLVSVLLFGA----LATRKRL---LTDRLFTATVAWPFPA 637
            D Q+SIP GT+ A + T+ +YL  ++LFGA    +  R +    L   L    +AWP P 
Sbjct: 454  DAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPW 513

Query: 638  IIYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYF-KVEDGCEPYFATLFTAILC 697
            +I +G   ST GA LQSLTGAPRLL AIA D I+P L  F   +   EP +A L T ++C
Sbjct: 514  VIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIVPFLQVFGHGKANGEPTWALLLTVLIC 573

Query: 698  IGCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASL 757
               ++I +LD + P ++MFFL+CY  VNL+C +  LL  P+WRPR+KF+HW+LS LG SL
Sbjct: 574  ETGILIASLDSVAPILSMFFLMCYLFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSL 633

Query: 758  CVVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQV 817
            C+ +MF+ SW + + ++ +A  IY Y+  RG   +WGDG +      A  +L  +     
Sbjct: 634  CLALMFICSWYYALSAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPP 693

Query: 818  HPKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVED 877
            H KNW P  L+         E    HP+L  F + + K G+G++I  S+L+G Y ++  +
Sbjct: 694  HTKNWRPQVLVML---NLDAEQAVKHPRLLSFTSQL-KAGKGLTIVGSVLEGTYLDKHME 753

Query: 878  SKAACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRR 937
            ++ A + + + +  ++ +G  ++VV+ S+ +G   ++Q+ GLG LK N ++M +P  W++
Sbjct: 754  AQRAEENIRSLMSTEKTKGFCQLVVSSSLRDGMSHLIQSAGLGGLKHNTVLMAWPASWKQ 813

Query: 938  ENLTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQR-QYGTIDLYWIVRDGGLMLLL 997
            E+       FV  + D   A++A+++ K +D +P   +R   G ID++WIV DGG+++LL
Sbjct: 814  EDNPFSWKNFVDTVRDTTAAHQALLVAKNVDSFPQNQERFGGGHIDVWWIVHDGGMLMLL 873

Query: 998  SQLLLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTT-- 1057
              LL   + +  C++++F +A+ D ++  +K D++ FLY LR+ AEV V+ +   D +  
Sbjct: 874  PFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAF 933

Query: 1058 -------QVEGGQQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNV---- 1079
                     +  Q  + ++     Q R A  + +   A+  + A    A   P  V    
Sbjct: 934  TYERTLMMEQRSQMLKQMQLSKNEQEREAQLIHDRNTASHTAAAARTQAPPTPDKVQMTW 993

BLAST of Cp4.1LG09g07280 vs. ExPASy Swiss-Prot
Match: Q9WVL3 (Solute carrier family 12 member 7 OS=Mus musculus OX=10090 GN=Slc12a7 PE=1 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-149
Identity = 331/1001 (33.07%), Postives = 530/1001 (52.95%), Query Frame = 0

Query: 218  SISQGLPKNEE-----------VKSGTLMGVFIPCLQNILGIIYYIRFSWIVGMAGIGES 277
            ++SQG+ ++EE            + GT +GV++PCLQNILG+I ++R +WIVG AG+ ES
Sbjct: 94   NLSQGVVEHEEDEDSRRREVKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGAAGVMES 153

Query: 278  LVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGLCFFLGNAVAG 337
             ++VA C  CT LT+IS+SAIATNG +  GG YY+I R+LGPE G ++GLCF+LG   AG
Sbjct: 154  FLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAG 213

Query: 338  ALYVLGAVETFLDAV-PSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGIIVTIFLCFIV 397
            A+Y+LG +E FL  + PSA IF+         T +   +   ++++VYG      +  +V
Sbjct: 214  AMYILGTIEIFLTYISPSAAIFQ-------AETADGEAAALLNNMRVYGSCALALMAVVV 273

Query: 398  FGGVKMINRVAPAFLIPVLFSVVCIFLGIF------------------LAKKN------- 457
            F GVK +N++A  FL  V+ S++ I+ G+                   LA +N       
Sbjct: 274  FVGVKYVNKLALVFLACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKM 333

Query: 458  -----------------------------------DPADGVTGLSMDTFKENWSSDY--- 517
                                                   G+ G++   F +N  S Y   
Sbjct: 334  QVVSNGTVTTALWRLFCNGSSLGATCDEYFAQNNVTEIQGIPGVASGVFLDNLWSTYSDK 393

Query: 518  ---------------QMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKD 577
                           + +   G+P    ++   F  LVG++FP+VTGIMAGSNRS  LKD
Sbjct: 394  GAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGDLKD 453

Query: 578  TQRSIPMGTLFATLLTTILYLVSVLLFGA----LATRKRL---LTDRLFTATVAWPFPAI 637
             Q+SIP GT+ A + T+ +YL  ++LFGA    +  R +    L   L    +AWP P +
Sbjct: 454  AQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWV 513

Query: 638  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYF-KVEDGCEPYFATLFTAILCI 697
            I +G   ST GA LQSLTGAPRLL AIA D I+P L  F   +   EP +A L TA++C 
Sbjct: 514  IVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTALICE 573

Query: 698  GCVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLC 757
              ++I +LD + P ++MFFL+CY  VNL+C +  LL  P+WRPR+KF+HW+LS LG SLC
Sbjct: 574  TGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLC 633

Query: 758  VVIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVH 817
            + +MF+ SW + + ++ +A  IY Y+  RG   +WGDG +      A  +L  +     H
Sbjct: 634  LALMFICSWYYALFAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPH 693

Query: 818  PKNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDS 877
             KNW P  L+         E    HP+L  F + + K G+G++I  S+L+G Y ++  ++
Sbjct: 694  TKNWRPQVLVML---NLDSEQCVKHPRLLSFTSQL-KAGKGLTIVGSVLEGTYLDKHVEA 753

Query: 878  KAACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRE 937
            + A + + + +  ++ +G  ++VV+ ++ +G   ++Q+ GLG +K N ++M +PE W+  
Sbjct: 754  QRAEENIRSLMSAEKTKGFCQLVVSSNLRDGASHLIQSAGLGGMKHNTVLMAWPEAWKEA 813

Query: 938  NLTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQR-QYGTIDLYWIVRDGGLMLLLS 997
            +       FV  + D   A++A+++ K +D +P   +R   G ID++WIV DGG+++LL 
Sbjct: 814  DNPFSWKNFVDTVRDTTAAHQALLVAKNIDLFPQNQERFSDGNIDVWWIVHDGGMLMLLP 873

Query: 998  QLLLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTT--- 1057
             LL   + +  C++++F +A+ D ++  +K D++ FLY LR+ AEV V+ +   D +   
Sbjct: 874  FLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFT 933

Query: 1058 ------QVEGGQQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNV----- 1079
                    +  Q  + ++     + R A  + +   A+  +      A   P  V     
Sbjct: 934  YEKTLMMEQRSQMLKQMQLSKNEREREAQLIHDRNTASHTTATARTQAPPTPDKVQMTWT 993

BLAST of Cp4.1LG09g07280 vs. NCBI nr
Match: XP_023541076.1 (cation-chloride cotransporter 1-like [Cucurbita pepo subsp. pepo] >KAG7012559.1 Cation-chloride cotransporter 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2070 bits (5362), Expect = 0.0
Identity = 1059/1078 (98.24%), Postives = 1059/1078 (98.24%), Query Frame = 0

Query: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60
            MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ
Sbjct: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60

Query: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120
            RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS
Sbjct: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120

Query: 121  TPSITTRHPSMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGE 180
            TPSITTRHPSMK                   KVKVGSQTGTDGKEGNSLTLVEINGPQGE
Sbjct: 121  TPSITTRHPSMK-------------------KVKVGSQTGTDGKEGNSLTLVEINGPQGE 180

Query: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240
            SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP
Sbjct: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240

Query: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300
            CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY
Sbjct: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300

Query: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360
            LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE
Sbjct: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360

Query: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420
            PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN
Sbjct: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420

Query: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480
            DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN
Sbjct: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480

Query: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540
            RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI
Sbjct: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540

Query: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600
            IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG
Sbjct: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600

Query: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660
            CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV
Sbjct: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660

Query: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720
            VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP
Sbjct: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720

Query: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780
            KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK
Sbjct: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780

Query: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840
            AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN
Sbjct: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840

Query: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900
            LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL
Sbjct: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900

Query: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960
            LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG
Sbjct: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960

Query: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020
            QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN
Sbjct: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020

Query: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT
Sbjct: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1059

BLAST of Cp4.1LG09g07280 vs. NCBI nr
Match: XP_022955334.1 (cation-chloride cotransporter 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 2068 bits (5358), Expect = 0.0
Identity = 1058/1078 (98.14%), Postives = 1059/1078 (98.24%), Query Frame = 0

Query: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60
            MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ
Sbjct: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60

Query: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120
            RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS
Sbjct: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120

Query: 121  TPSITTRHPSMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGE 180
            TPSITTRHPSMK                   KVKVGSQTGTDGKEGNSLTLVEINGPQGE
Sbjct: 121  TPSITTRHPSMK-------------------KVKVGSQTGTDGKEGNSLTLVEINGPQGE 180

Query: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240
            SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP
Sbjct: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240

Query: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300
            CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY
Sbjct: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300

Query: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360
            LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE
Sbjct: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360

Query: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420
            PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN
Sbjct: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420

Query: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480
            DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN
Sbjct: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480

Query: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540
            RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI
Sbjct: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540

Query: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600
            IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG
Sbjct: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600

Query: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660
            CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV
Sbjct: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660

Query: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720
            VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP
Sbjct: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720

Query: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780
            KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK
Sbjct: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780

Query: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840
            AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN
Sbjct: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840

Query: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900
            LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL
Sbjct: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900

Query: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960
            LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG
Sbjct: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960

Query: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020
            QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTL+ADGKPVNVNEKQVQKFLNTTLKLN
Sbjct: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLVADGKPVNVNEKQVQKFLNTTLKLN 1020

Query: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT
Sbjct: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1059

BLAST of Cp4.1LG09g07280 vs. NCBI nr
Match: XP_022994722.1 (cation-chloride cotransporter 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 2060 bits (5336), Expect = 0.0
Identity = 1053/1078 (97.68%), Postives = 1056/1078 (97.96%), Query Frame = 0

Query: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60
            MSLSPLFFSSF+SSFNFSPWSPTSFACFLLYTLS TWICISFPVFVLDLHLVL+FTDSLQ
Sbjct: 1    MSLSPLFFSSFTSSFNFSPWSPTSFACFLLYTLSLTWICISFPVFVLDLHLVLIFTDSLQ 60

Query: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120
            RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS
Sbjct: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120

Query: 121  TPSITTRHPSMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGE 180
            T SITTRHPSMK                   KVKVGSQTGTDGKEGNSLTLVEINGPQGE
Sbjct: 121  TSSITTRHPSMK-------------------KVKVGSQTGTDGKEGNSLTLVEINGPQGE 180

Query: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240
            SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP
Sbjct: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240

Query: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300
            CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY
Sbjct: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300

Query: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360
            LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE
Sbjct: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360

Query: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420
            PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN
Sbjct: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420

Query: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480
            DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN
Sbjct: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480

Query: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540
            RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI
Sbjct: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540

Query: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600
            IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYF+VEDGCEPYFATLFTAILCIG
Sbjct: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFQVEDGCEPYFATLFTAILCIG 600

Query: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660
            CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV
Sbjct: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660

Query: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720
            VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP
Sbjct: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720

Query: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780
            KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK
Sbjct: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780

Query: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840
            AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN
Sbjct: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840

Query: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900
            LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL
Sbjct: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900

Query: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960
            LLTKESFESCKIQVFCIAEEDSDAE LKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG
Sbjct: 901  LLTKESFESCKIQVFCIAEEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960

Query: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020
            QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN
Sbjct: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020

Query: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT
Sbjct: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1059

BLAST of Cp4.1LG09g07280 vs. NCBI nr
Match: KAG6573392.1 (Cation-chloride cotransporter 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1924 bits (4985), Expect = 0.0
Identity = 982/1002 (98.00%), Postives = 983/1002 (98.10%), Query Frame = 0

Query: 77   MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHPSMKVNFG 136
            MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHPSMK    
Sbjct: 1    MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHPSMK---- 60

Query: 137  FGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNILG 196
                           KVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNILG
Sbjct: 61   ---------------KVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNILG 120

Query: 197  LKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFSW 256
            LKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFSW
Sbjct: 121  LKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFSW 180

Query: 257  IVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 316
            IVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL
Sbjct: 181  IVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 240

Query: 317  CFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGII 376
            CFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGII
Sbjct: 241  CFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGII 300

Query: 377  VTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFKE 436
            VTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFKE
Sbjct: 301  VTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFKE 360

Query: 437  NWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMGT 496
            NWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMGT
Sbjct: 361  NWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMGT 420

Query: 497  LFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQS 556
            LFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQS
Sbjct: 421  LFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQS 480

Query: 557  LTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVTM 616
            LTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVTM
Sbjct: 481  LTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVTM 540

Query: 617  FFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSLA 676
            FFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSLA
Sbjct: 541  FFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSLA 600

Query: 677  LASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGK 736
            LASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGK
Sbjct: 601  LASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGK 660

Query: 737  LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRCE 796
            LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRCE
Sbjct: 661  LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRCE 720

Query: 797  GVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDCI 856
            GVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDCI
Sbjct: 721  GVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDCI 780

Query: 857  DANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFC 916
            DANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFC
Sbjct: 781  DANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFC 840

Query: 917  IAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGRI 976
            IAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGRI
Sbjct: 841  IAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGRI 900

Query: 977  ASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLVS 1036
            ASYLGEMKAAAAESRATTL+ADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLVS
Sbjct: 901  ASYLGEMKAAAAESRATTLVADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLVS 960

Query: 1037 LPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            LPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT
Sbjct: 961  LPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 983

BLAST of Cp4.1LG09g07280 vs. NCBI nr
Match: XP_011659141.1 (cation-chloride cotransporter 1 isoform X1 [Cucumis sativus] >KGN65931.1 hypothetical protein Csa_023216 [Cucumis sativus])

HSP 1 Score: 1791 bits (4638), Expect = 0.0
Identity = 915/1003 (91.23%), Postives = 950/1003 (94.72%), Query Frame = 0

Query: 77   MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITT-RHPSMKVNF 136
            MDN DIESGEE+FHGQRGRKYRPVEAHDRAVLEMSSMDPG +SS+ S+   RHPS+K   
Sbjct: 1    MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSVPPIRHPSLK--- 60

Query: 137  GFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNIL 196
                            KVKVGSQTGT+ KEGNS T +++NGPQ ESKLEFFGFDSLVNIL
Sbjct: 61   ----------------KVKVGSQTGTE-KEGNSPTRIDVNGPQRESKLEFFGFDSLVNIL 120

Query: 197  GLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFS 256
            GLKSMMGEQIQAPSSPRDGENVS++QGLPK  EVKSGTLMGVFIPCLQNILGIIYYIRFS
Sbjct: 121  GLKSMMGEQIQAPSSPRDGENVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFS 180

Query: 257  WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG 316
            WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG
Sbjct: 181  WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG 240

Query: 317  LCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGI 376
            LCFFLGNAVAGALYVLGAVETFL+AVP+AGIFRETVTKVNG+TVEPIQSPSSHDLQVYGI
Sbjct: 241  LCFFLGNAVAGALYVLGAVETFLNAVPAAGIFRETVTKVNGTTVEPIQSPSSHDLQVYGI 300

Query: 377  IVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFK 436
            IVTI LCFIVFGGVKMINRVAPAFLIPVLFSV+CIFLG+FLA KNDP +GVTGLS+++FK
Sbjct: 301  IVTILLCFIVFGGVKMINRVAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFK 360

Query: 437  ENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMG 496
            ENWSSDYQMTNSAGIPDP G VYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIP+G
Sbjct: 361  ENWSSDYQMTNSAGIPDPLGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIG 420

Query: 497  TLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQ 556
            TL ATL TT++YLVSVLLFGALATRK+LLTDRL TATVAWPFPAIIYVGIILSTLGAALQ
Sbjct: 421  TLAATLTTTVMYLVSVLLFGALATRKKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQ 480

Query: 557  SLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVT 616
            SLTGAPRLLAAIAND+ILPILNYFKV DG EPYFATLFTA LCIGCVIIGNLDLITPTVT
Sbjct: 481  SLTGAPRLLAAIANDDILPILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVT 540

Query: 617  MFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSL 676
            MFFLLCYAGVNLSCFLLDLLDAPSWRPRW+FHHWSLSLLGASLCVVIMFLISWSFTIVSL
Sbjct: 541  MFFLLCYAGVNLSCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSL 600

Query: 677  ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG 736
            ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG
Sbjct: 601  ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG 660

Query: 737  KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRC 796
            KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRC
Sbjct: 661  KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRC 720

Query: 797  EGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDC 856
            EGVAEIVVAP+MSEGFRGIVQTMGLGNLKPNI+VMRYPEIWRRENL EIPATFVGIINDC
Sbjct: 721  EGVAEIVVAPTMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLIEIPATFVGIINDC 780

Query: 857  IDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF 916
            IDANKAVVIVKGLDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF
Sbjct: 781  IDANKAVVIVKGLDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF 840

Query: 917  CIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGR 976
            CIAEEDSDAE LKADVKKFLYDLRMQAEVIVITIKSWDT QVEGGQQDES+EAFTA QGR
Sbjct: 841  CIAEEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDT-QVEGGQQDESMEAFTAAQGR 900

Query: 977  IASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLV 1036
            IASYL EMK  A ESR TTLMADGKPV+VNE+QV+KFLNTTLKLNS+ILRYSRMAAVVLV
Sbjct: 901  IASYLSEMKETA-ESRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLV 960

Query: 1037 SLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SLPPPP NHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTL+T
Sbjct: 961  SLPPPPVNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLFT 981

BLAST of Cp4.1LG09g07280 vs. ExPASy TrEMBL
Match: A0A6J1GVY5 (cation-chloride cotransporter 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457327 PE=3 SV=1)

HSP 1 Score: 2068 bits (5358), Expect = 0.0
Identity = 1058/1078 (98.14%), Postives = 1059/1078 (98.24%), Query Frame = 0

Query: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60
            MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ
Sbjct: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60

Query: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120
            RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS
Sbjct: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120

Query: 121  TPSITTRHPSMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGE 180
            TPSITTRHPSMK                   KVKVGSQTGTDGKEGNSLTLVEINGPQGE
Sbjct: 121  TPSITTRHPSMK-------------------KVKVGSQTGTDGKEGNSLTLVEINGPQGE 180

Query: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240
            SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP
Sbjct: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240

Query: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300
            CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY
Sbjct: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300

Query: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360
            LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE
Sbjct: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360

Query: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420
            PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN
Sbjct: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420

Query: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480
            DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN
Sbjct: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480

Query: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540
            RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI
Sbjct: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540

Query: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600
            IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG
Sbjct: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600

Query: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660
            CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV
Sbjct: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660

Query: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720
            VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP
Sbjct: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720

Query: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780
            KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK
Sbjct: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780

Query: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840
            AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN
Sbjct: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840

Query: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900
            LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL
Sbjct: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900

Query: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960
            LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG
Sbjct: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960

Query: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020
            QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTL+ADGKPVNVNEKQVQKFLNTTLKLN
Sbjct: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLVADGKPVNVNEKQVQKFLNTTLKLN 1020

Query: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT
Sbjct: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1059

BLAST of Cp4.1LG09g07280 vs. ExPASy TrEMBL
Match: A0A6J1JWN5 (cation-chloride cotransporter 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490367 PE=3 SV=1)

HSP 1 Score: 2060 bits (5336), Expect = 0.0
Identity = 1053/1078 (97.68%), Postives = 1056/1078 (97.96%), Query Frame = 0

Query: 1    MSLSPLFFSSFSSSFNFSPWSPTSFACFLLYTLSFTWICISFPVFVLDLHLVLVFTDSLQ 60
            MSLSPLFFSSF+SSFNFSPWSPTSFACFLLYTLS TWICISFPVFVLDLHLVL+FTDSLQ
Sbjct: 1    MSLSPLFFSSFTSSFNFSPWSPTSFACFLLYTLSLTWICISFPVFVLDLHLVLIFTDSLQ 60

Query: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120
            RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS
Sbjct: 61   RKSGELSWNSILACSEMDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASS 120

Query: 121  TPSITTRHPSMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGE 180
            T SITTRHPSMK                   KVKVGSQTGTDGKEGNSLTLVEINGPQGE
Sbjct: 121  TSSITTRHPSMK-------------------KVKVGSQTGTDGKEGNSLTLVEINGPQGE 180

Query: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240
            SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP
Sbjct: 181  SKLEFFGFDSLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIP 240

Query: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300
            CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY
Sbjct: 241  CLQNILGIIYYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYY 300

Query: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360
            LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE
Sbjct: 301  LIGRALGPEVGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVE 360

Query: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420
            PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN
Sbjct: 361  PIQSPSSHDLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKN 420

Query: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480
            DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN
Sbjct: 421  DPADGVTGLSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSN 480

Query: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540
            RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI
Sbjct: 481  RSASLKDTQRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAI 540

Query: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIG 600
            IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYF+VEDGCEPYFATLFTAILCIG
Sbjct: 541  IYVGIILSTLGAALQSLTGAPRLLAAIANDNILPILNYFQVEDGCEPYFATLFTAILCIG 600

Query: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660
            CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV
Sbjct: 601  CVIIGNLDLITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCV 660

Query: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720
            VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP
Sbjct: 661  VIMFLISWSFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHP 720

Query: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780
            KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK
Sbjct: 721  KNWYPIPLIFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK 780

Query: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840
            AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN
Sbjct: 781  AACKQLATYIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRREN 840

Query: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900
            LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL
Sbjct: 841  LTEIPATFVGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQL 900

Query: 901  LLTKESFESCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960
            LLTKESFESCKIQVFCIAEEDSDAE LKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG
Sbjct: 901  LLTKESFESCKIQVFCIAEEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGG 960

Query: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020
            QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN
Sbjct: 961  QQDESIEAFTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLN 1020

Query: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT
Sbjct: 1021 SSILRYSRMAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1059

BLAST of Cp4.1LG09g07280 vs. ExPASy TrEMBL
Match: A0A0A0M162 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G538780 PE=3 SV=1)

HSP 1 Score: 1791 bits (4638), Expect = 0.0
Identity = 915/1003 (91.23%), Postives = 950/1003 (94.72%), Query Frame = 0

Query: 77   MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITT-RHPSMKVNF 136
            MDN DIESGEE+FHGQRGRKYRPVEAHDRAVLEMSSMDPG +SS+ S+   RHPS+K   
Sbjct: 1    MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSVPPIRHPSLK--- 60

Query: 137  GFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNIL 196
                            KVKVGSQTGT+ KEGNS T +++NGPQ ESKLEFFGFDSLVNIL
Sbjct: 61   ----------------KVKVGSQTGTE-KEGNSPTRIDVNGPQRESKLEFFGFDSLVNIL 120

Query: 197  GLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFS 256
            GLKSMMGEQIQAPSSPRDGENVS++QGLPK  EVKSGTLMGVFIPCLQNILGIIYYIRFS
Sbjct: 121  GLKSMMGEQIQAPSSPRDGENVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFS 180

Query: 257  WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG 316
            WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG
Sbjct: 181  WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG 240

Query: 317  LCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGI 376
            LCFFLGNAVAGALYVLGAVETFL+AVP+AGIFRETVTKVNG+TVEPIQSPSSHDLQVYGI
Sbjct: 241  LCFFLGNAVAGALYVLGAVETFLNAVPAAGIFRETVTKVNGTTVEPIQSPSSHDLQVYGI 300

Query: 377  IVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFK 436
            IVTI LCFIVFGGVKMINRVAPAFLIPVLFSV+CIFLG+FLA KNDP +GVTGLS+++FK
Sbjct: 301  IVTILLCFIVFGGVKMINRVAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFK 360

Query: 437  ENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMG 496
            ENWSSDYQMTNSAGIPDP G VYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIP+G
Sbjct: 361  ENWSSDYQMTNSAGIPDPLGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIG 420

Query: 497  TLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQ 556
            TL ATL TT++YLVSVLLFGALATRK+LLTDRL TATVAWPFPAIIYVGIILSTLGAALQ
Sbjct: 421  TLAATLTTTVMYLVSVLLFGALATRKKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQ 480

Query: 557  SLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVT 616
            SLTGAPRLLAAIAND+ILPILNYFKV DG EPYFATLFTA LCIGCVIIGNLDLITPTVT
Sbjct: 481  SLTGAPRLLAAIANDDILPILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVT 540

Query: 617  MFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSL 676
            MFFLLCYAGVNLSCFLLDLLDAPSWRPRW+FHHWSLSLLGASLCVVIMFLISWSFTIVSL
Sbjct: 541  MFFLLCYAGVNLSCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSL 600

Query: 677  ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG 736
            ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG
Sbjct: 601  ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG 660

Query: 737  KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRC 796
            KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRC
Sbjct: 661  KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRC 720

Query: 797  EGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDC 856
            EGVAEIVVAP+MSEGFRGIVQTMGLGNLKPNI+VMRYPEIWRRENL EIPATFVGIINDC
Sbjct: 721  EGVAEIVVAPTMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLIEIPATFVGIINDC 780

Query: 857  IDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF 916
            IDANKAVVIVKGLDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF
Sbjct: 781  IDANKAVVIVKGLDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF 840

Query: 917  CIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGR 976
            CIAEEDSDAE LKADVKKFLYDLRMQAEVIVITIKSWDT QVEGGQQDES+EAFTA QGR
Sbjct: 841  CIAEEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDT-QVEGGQQDESMEAFTAAQGR 900

Query: 977  IASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLV 1036
            IASYL EMK  A ESR TTLMADGKPV+VNE+QV+KFLNTTLKLNS+ILRYSRMAAVVLV
Sbjct: 901  IASYLSEMKETA-ESRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLV 960

Query: 1037 SLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SLPPPP NHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTL+T
Sbjct: 961  SLPPPPVNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLFT 981

BLAST of Cp4.1LG09g07280 vs. ExPASy TrEMBL
Match: A0A1S3BBY4 (cation-chloride cotransporter 1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488259 PE=3 SV=1)

HSP 1 Score: 1791 bits (4638), Expect = 0.0
Identity = 917/1003 (91.43%), Postives = 950/1003 (94.72%), Query Frame = 0

Query: 77   MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITT-RHPSMKVNF 136
            MDN DIESGEE+FHGQRGRKYRPVEAHDRAVLEMSSMDPG +SS+ SI   RHPS+K   
Sbjct: 1    MDNGDIESGEEEFHGQRGRKYRPVEAHDRAVLEMSSMDPGSSSSSSSIPPIRHPSLK--- 60

Query: 137  GFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNIL 196
                            KVKVGSQTGT+ K+GNS T +E+NGPQ ESKLEFFGFDSLVNIL
Sbjct: 61   ----------------KVKVGSQTGTE-KDGNSPTRIEVNGPQRESKLEFFGFDSLVNIL 120

Query: 197  GLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFS 256
            GLKSMMGEQIQAPSSPRDGENVS++QGLPK  EVKSGTLMGVFIPCLQNILGIIYYIRFS
Sbjct: 121  GLKSMMGEQIQAPSSPRDGENVSMTQGLPKTNEVKSGTLMGVFIPCLQNILGIIYYIRFS 180

Query: 257  WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG 316
            WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG
Sbjct: 181  WIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIG 240

Query: 317  LCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGI 376
            LCFFLGNAVAGALYVLGAVETFL+AVP+AGIFRETVTKVNG+TVEPIQSPSSHDLQVYGI
Sbjct: 241  LCFFLGNAVAGALYVLGAVETFLNAVPAAGIFRETVTKVNGTTVEPIQSPSSHDLQVYGI 300

Query: 377  IVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFK 436
            IVTI LCFIVFGGVKMINRVAPAFLIPVLFSV+CIFLG+FLA KNDP +GVTGLS+++FK
Sbjct: 301  IVTILLCFIVFGGVKMINRVAPAFLIPVLFSVLCIFLGVFLAGKNDPTEGVTGLSLESFK 360

Query: 437  ENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMG 496
            ENWSSDYQMTNSAGIPDP G VYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIP+G
Sbjct: 361  ENWSSDYQMTNSAGIPDPLGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIG 420

Query: 497  TLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQ 556
            TL ATL TT LYLVSVLLFGALATR++LLTDRL TATVAWPFPAIIYVGIILSTLGAALQ
Sbjct: 421  TLAATLTTTALYLVSVLLFGALATREKLLTDRLLTATVAWPFPAIIYVGIILSTLGAALQ 480

Query: 557  SLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVT 616
            SLTGAPRLLAAIAND+ILPILNYFKV DG EPYFATLFTA LCIGCVIIGNLDLITPTVT
Sbjct: 481  SLTGAPRLLAAIANDDILPILNYFKVADGSEPYFATLFTAFLCIGCVIIGNLDLITPTVT 540

Query: 617  MFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSL 676
            MFFLLCYAGVNLSCFLLDLLDAPSWRPRW+FHHWSLSLLGASLCVVIMFLISWSFTIVSL
Sbjct: 541  MFFLLCYAGVNLSCFLLDLLDAPSWRPRWRFHHWSLSLLGASLCVVIMFLISWSFTIVSL 600

Query: 677  ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG 736
            ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG
Sbjct: 601  ALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWG 660

Query: 737  KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRC 796
            KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVED KAACKQLATYIDYKRC
Sbjct: 661  KLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDGKAACKQLATYIDYKRC 720

Query: 797  EGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDC 856
            EGVAEIVVAP+MSEGFRGIVQTMGLGNLKPNI+VMRYPEIWRRENLTEIPATFVGIINDC
Sbjct: 721  EGVAEIVVAPTMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLTEIPATFVGIINDC 780

Query: 857  IDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF 916
            IDANKAVVIVKGLDEWPNE+QRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF
Sbjct: 781  IDANKAVVIVKGLDEWPNEFQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVF 840

Query: 917  CIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGR 976
            CIAEEDSDAE LKADVKKFLYDLRMQAEVIVITIKSWDT QVEG QQDES+EAFTA QGR
Sbjct: 841  CIAEEDSDAEGLKADVKKFLYDLRMQAEVIVITIKSWDT-QVEGAQQDESMEAFTAAQGR 900

Query: 977  IASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLV 1036
            IASYL EMKA A ESR TTLMADGKPV+VNE+QV+KFLNTTLKLNS+ILRYSRMAAVVLV
Sbjct: 901  IASYLSEMKATA-ESRGTTLMADGKPVSVNEQQVEKFLNTTLKLNSTILRYSRMAAVVLV 960

Query: 1037 SLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            SLPPPP NHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTL+T
Sbjct: 961  SLPPPPVNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLFT 981

BLAST of Cp4.1LG09g07280 vs. ExPASy TrEMBL
Match: A0A6J1CH20 (cation-chloride cotransporter 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010778 PE=3 SV=1)

HSP 1 Score: 1785 bits (4622), Expect = 0.0
Identity = 907/1002 (90.52%), Postives = 948/1002 (94.61%), Query Frame = 0

Query: 77   MDNADIESGEEDFHGQRGRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHPSMKVNFG 136
            MDNADIESGEEDFHG+RGRKYRPVEAHDRAVLEMSSMDPG +SS+PS+  RHPSMK    
Sbjct: 1    MDNADIESGEEDFHGKRGRKYRPVEAHDRAVLEMSSMDPGSSSSSPSVPMRHPSMK---- 60

Query: 137  FGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFDSLVNILG 196
                           KVKVGSQ G+D KEGNS + +E+NGPQ +SKLEFFGFDSLVNILG
Sbjct: 61   ---------------KVKVGSQGGSDEKEGNSPSRIEVNGPQRDSKLEFFGFDSLVNILG 120

Query: 197  LKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGIIYYIRFSW 256
            LKSMMGEQIQAPSSPRDGE+V I+QGLPK  EVKSGT+MGVFIPCLQNILGIIYYIRF+W
Sbjct: 121  LKSMMGEQIQAPSSPRDGESVPITQGLPKTNEVKSGTMMGVFIPCLQNILGIIYYIRFTW 180

Query: 257  IVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 316
            IVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL
Sbjct: 181  IVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPEVGVSIGL 240

Query: 317  CFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTVEPIQSPSSHDLQVYGII 376
            CFFLGNAVAGALYVLGAVETFL+AVP+AGIFRE VTKVNG+TV PIQSPSSHDLQVYGII
Sbjct: 241  CFFLGNAVAGALYVLGAVETFLNAVPAAGIFREAVTKVNGTTVAPIQSPSSHDLQVYGII 300

Query: 377  VTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTGLSMDTFKE 436
            VTI LCFIVFGGVKMINRVAPAFLIPVLFS++CIFLG+FLA KNDPADGVTGLS+++FKE
Sbjct: 301  VTILLCFIVFGGVKMINRVAPAFLIPVLFSLLCIFLGVFLAGKNDPADGVTGLSLESFKE 360

Query: 437  NWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPMGT 496
            NWSSDYQMTNSAGIPDP G VYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIP+GT
Sbjct: 361  NWSSDYQMTNSAGIPDPLGKVYWNFNALVGLFFPAVTGIMAGSNRSASLKDTQRSIPIGT 420

Query: 497  LFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILSTLGAALQS 556
            L AT +TT LYLVSVLLFGALATR++LLTDRL TAT+AWPFPAIIYVGIILSTLGAALQS
Sbjct: 421  LAATFVTTSLYLVSVLLFGALATREKLLTDRLLTATIAWPFPAIIYVGIILSTLGAALQS 480

Query: 557  LTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLDLITPTVTM 616
            LTGAPRLLAAIAND+ILPILNYFKV DG EPYFATLFTA LC+GCVIIGNLDLITPTVTM
Sbjct: 481  LTGAPRLLAAIANDDILPILNYFKVADGSEPYFATLFTAFLCVGCVIIGNLDLITPTVTM 540

Query: 617  FFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVSLA 676
            FFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIV+LA
Sbjct: 541  FFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISWSFTIVALA 600

Query: 677  LASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGK 736
            LASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGK
Sbjct: 601  LASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPLIFCRPWGK 660

Query: 737  LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLATYIDYKRCE 796
            LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSK+ACKQLATYIDYKRCE
Sbjct: 661  LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKSACKQLATYIDYKRCE 720

Query: 797  GVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATFVGIINDCI 856
            GVAEIVVAPSMSEGFRGIVQTMGLGNLKPNI+VMRYPEIWRRENLTEIPATFVGIINDCI
Sbjct: 721  GVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIVVMRYPEIWRRENLTEIPATFVGIINDCI 780

Query: 857  DANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFESCKIQVFC 916
            DANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTK SFESCKIQVFC
Sbjct: 781  DANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKASFESCKIQVFC 840

Query: 917  IAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEAFTAGQGRI 976
            IAEE+SDAE LKADVKKFLYDLRMQAEVIVITIKSW   Q +GGQ DES+EAFTA QGRI
Sbjct: 841  IAEEESDAEGLKADVKKFLYDLRMQAEVIVITIKSWGV-QGDGGQPDESMEAFTAAQGRI 900

Query: 977  ASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSRMAAVVLVS 1036
            ASYLGEMKAAA ESR TTLMADGKPV+VNEKQV+KFL+TTLKLN++ILRYSRMAAVVLVS
Sbjct: 901  ASYLGEMKAAA-ESRGTTLMADGKPVSVNEKQVEKFLDTTLKLNTTILRYSRMAAVVLVS 960

Query: 1037 LPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1078
            LPPPP NHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTL+T
Sbjct: 961  LPPPPVNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLFT 981

BLAST of Cp4.1LG09g07280 vs. TAIR 10
Match: AT1G30450.1 (cation-chloride co-transporter 1 )

HSP 1 Score: 1556.6 bits (4029), Expect = 0.0e+00
Identity = 793/1010 (78.51%), Postives = 879/1010 (87.03%), Query Frame = 0

Query: 77   MDNADIE----SGEEDFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHP 136
            MD+ DIE    +GEE+F  G R  G KYRPV AHDRAV+EMSS+DPG +SST        
Sbjct: 1    MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSST-------- 60

Query: 137  SMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFD 196
                          L     V    VG+  G  G E        +NG Q ESKLE FGFD
Sbjct: 61   --------------LKNIKVVAPGDVGA--GVRGPEDG------VNGHQKESKLELFGFD 120

Query: 197  SLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGII 256
            SLVNILGLKSM GEQIQAPSSPRDGE++SI+QG PK   +K GT+MGVF+PCLQNILGII
Sbjct: 121  SLVNILGLKSMTGEQIQAPSSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGII 180

Query: 257  YYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPE 316
            YYIRF+WIVGMAGIG+ LVLV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPE
Sbjct: 181  YYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPE 240

Query: 317  VGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTV-EPIQSPSSH 376
            VG+SIGLCFFLGNAVAGALYVLGAVETFL A P+AGIFRET+TKVNG+ V E IQSP+SH
Sbjct: 241  VGISIGLCFFLGNAVAGALYVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSH 300

Query: 377  DLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTG 436
            DLQVYGI+VTI LCFIVFGGVKMINRVAPAFL+PVL S+ CIF+GIFLAK +DP +G+TG
Sbjct: 301  DLQVYGIVVTILLCFIVFGGVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITG 360

Query: 437  LSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDT 496
            L + +FK+NW S YQMTN AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDT
Sbjct: 361  LRLKSFKDNWGSAYQMTNDAGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDT 420

Query: 497  QRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILS 556
            Q+SIP+GTL ATL TT LYL+SVL FGA+ATR +LLTDRL TAT+AWPFPAI++VGIILS
Sbjct: 421  QKSIPVGTLAATLTTTSLYLISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILS 480

Query: 557  TLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLD 616
            TLGAALQSLTGAPRLLAAIAND+ILPILNYFKV D  EP+ ATLFTA +CIGCV+IGNLD
Sbjct: 481  TLGAALQSLTGAPRLLAAIANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLD 540

Query: 617  LITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISW 676
            LITPTVTMF+LLCY+GVNLSCFLLDLLDAPSWRPRWK+HHWSLS +GASLC+VIMFLISW
Sbjct: 541  LITPTVTMFYLLCYSGVNLSCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISW 600

Query: 677  SFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPL 736
            SFT+V++ALASLIY YV L+GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL
Sbjct: 601  SFTVVAIALASLIYKYVGLKGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPL 660

Query: 737  IFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLAT 796
            +FCRPWG+LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDY+E  E++K ACKQLAT
Sbjct: 661  VFCRPWGQLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLAT 720

Query: 797  YIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATF 856
            YI+YKRCEGVAEIVVAP+M+EGFRGI+QTMGLGNLKPNI+VMRYPEIWRRENLTEIP+TF
Sbjct: 721  YIEYKRCEGVAEIVVAPNMTEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTF 780

Query: 857  VGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 916
            VGIINDCI ANKAVVI+KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE
Sbjct: 781  VGIINDCITANKAVVIIKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 840

Query: 917  SCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEA 976
            SCKIQ+FCIAEEDSDAEALKADVKKFLYDLRM AEVIV+T+KSWD  + EG  Q++S+EA
Sbjct: 841  SCKIQLFCIAEEDSDAEALKADVKKFLYDLRMHAEVIVVTMKSWD-IRSEGNSQEDSLEA 900

Query: 977  FTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSR 1036
            F A Q RI+ YLGE+K       +  L+A+GKP+ VNE+QV+KFL T LKLNS+IL YSR
Sbjct: 901  FDAAQRRISDYLGEIK----RQGSNPLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSR 960

Query: 1037 MAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1079
            MAAVVLVSLPPPP NHPAYFYMEY+DLLVENVPR+LIVRGY RDVVTL+T
Sbjct: 961  MAAVVLVSLPPPPLNHPAYFYMEYMDLLVENVPRMLIVRGYHRDVVTLFT 975

BLAST of Cp4.1LG09g07280 vs. TAIR 10
Match: AT1G30450.3 (cation-chloride co-transporter 1 )

HSP 1 Score: 1556.6 bits (4029), Expect = 0.0e+00
Identity = 793/1010 (78.51%), Postives = 879/1010 (87.03%), Query Frame = 0

Query: 77   MDNADIE----SGEEDFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHP 136
            MD+ DIE    +GEE+F  G R  G KYRPV AHDRAV+EMSS+DPG +SST        
Sbjct: 1    MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSST-------- 60

Query: 137  SMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFD 196
                          L     V    VG+  G  G E        +NG Q ESKLE FGFD
Sbjct: 61   --------------LKNIKVVAPGDVGA--GVRGPEDG------VNGHQKESKLELFGFD 120

Query: 197  SLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGII 256
            SLVNILGLKSM GEQIQAPSSPRDGE++SI+QG PK   +K GT+MGVF+PCLQNILGII
Sbjct: 121  SLVNILGLKSMTGEQIQAPSSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGII 180

Query: 257  YYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPE 316
            YYIRF+WIVGMAGIG+ LVLV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPE
Sbjct: 181  YYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPE 240

Query: 317  VGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTV-EPIQSPSSH 376
            VG+SIGLCFFLGNAVAGALYVLGAVETFL A P+AGIFRET+TKVNG+ V E IQSP+SH
Sbjct: 241  VGISIGLCFFLGNAVAGALYVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSH 300

Query: 377  DLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTG 436
            DLQVYGI+VTI LCFIVFGGVKMINRVAPAFL+PVL S+ CIF+GIFLAK +DP +G+TG
Sbjct: 301  DLQVYGIVVTILLCFIVFGGVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITG 360

Query: 437  LSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDT 496
            L + +FK+NW S YQMTN AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDT
Sbjct: 361  LRLKSFKDNWGSAYQMTNDAGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDT 420

Query: 497  QRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILS 556
            Q+SIP+GTL ATL TT LYL+SVL FGA+ATR +LLTDRL TAT+AWPFPAI++VGIILS
Sbjct: 421  QKSIPVGTLAATLTTTSLYLISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILS 480

Query: 557  TLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLD 616
            TLGAALQSLTGAPRLLAAIAND+ILPILNYFKV D  EP+ ATLFTA +CIGCV+IGNLD
Sbjct: 481  TLGAALQSLTGAPRLLAAIANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLD 540

Query: 617  LITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISW 676
            LITPTVTMF+LLCY+GVNLSCFLLDLLDAPSWRPRWK+HHWSLS +GASLC+VIMFLISW
Sbjct: 541  LITPTVTMFYLLCYSGVNLSCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISW 600

Query: 677  SFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPL 736
            SFT+V++ALASLIY YV L+GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL
Sbjct: 601  SFTVVAIALASLIYKYVGLKGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPL 660

Query: 737  IFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLAT 796
            +FCRPWG+LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDY+E  E++K ACKQLAT
Sbjct: 661  VFCRPWGQLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLAT 720

Query: 797  YIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATF 856
            YI+YKRCEGVAEIVVAP+M+EGFRGI+QTMGLGNLKPNI+VMRYPEIWRRENLTEIP+TF
Sbjct: 721  YIEYKRCEGVAEIVVAPNMTEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTF 780

Query: 857  VGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 916
            VGIINDCI ANKAVVI+KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE
Sbjct: 781  VGIINDCITANKAVVIIKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 840

Query: 917  SCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEA 976
            SCKIQ+FCIAEEDSDAEALKADVKKFLYDLRM AEVIV+T+KSWD  + EG  Q++S+EA
Sbjct: 841  SCKIQLFCIAEEDSDAEALKADVKKFLYDLRMHAEVIVVTMKSWD-IRSEGNSQEDSLEA 900

Query: 977  FTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSR 1036
            F A Q RI+ YLGE+K       +  L+A+GKP+ VNE+QV+KFL T LKLNS+IL YSR
Sbjct: 901  FDAAQRRISDYLGEIK----RQGSNPLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSR 960

Query: 1037 MAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1079
            MAAVVLVSLPPPP NHPAYFYMEY+DLLVENVPR+LIVRGY RDVVTL+T
Sbjct: 961  MAAVVLVSLPPPPLNHPAYFYMEYMDLLVENVPRMLIVRGYHRDVVTLFT 975

BLAST of Cp4.1LG09g07280 vs. TAIR 10
Match: AT1G30450.2 (cation-chloride co-transporter 1 )

HSP 1 Score: 1556.6 bits (4029), Expect = 0.0e+00
Identity = 793/1010 (78.51%), Postives = 879/1010 (87.03%), Query Frame = 0

Query: 77   MDNADIE----SGEEDFH-GQR--GRKYRPVEAHDRAVLEMSSMDPGPASSTPSITTRHP 136
            MD+ DIE    +GEE+F  G R  G KYRPV AHDRAV+EMSS+DPG +SST        
Sbjct: 1    MDSGDIEEAGGNGEEEFRSGPRLGGSKYRPVVAHDRAVVEMSSIDPGSSSST-------- 60

Query: 137  SMKVNFGFGFAALFLNRYHDVMKVKVGSQTGTDGKEGNSLTLVEINGPQGESKLEFFGFD 196
                          L     V    VG+  G  G E        +NG Q ESKLE FGFD
Sbjct: 61   --------------LKNIKVVAPGDVGA--GVRGPEDG------VNGHQKESKLELFGFD 120

Query: 197  SLVNILGLKSMMGEQIQAPSSPRDGENVSISQGLPKNEEVKSGTLMGVFIPCLQNILGII 256
            SLVNILGLKSM GEQIQAPSSPRDGE++SI+QG PK   +K GT+MGVF+PCLQNILGII
Sbjct: 121  SLVNILGLKSMTGEQIQAPSSPRDGEDISITQGHPKPPALKMGTMMGVFVPCLQNILGII 180

Query: 257  YYIRFSWIVGMAGIGESLVLVAFCGLCTFLTSISLSAIATNGAMKGGGPYYLIGRALGPE 316
            YYIRF+WIVGMAGIG+ LVLV  CGLCTFLT+ISLSAIATNGAMKGGGPYYLIGRALGPE
Sbjct: 181  YYIRFTWIVGMAGIGQGLVLVFLCGLCTFLTTISLSAIATNGAMKGGGPYYLIGRALGPE 240

Query: 317  VGVSIGLCFFLGNAVAGALYVLGAVETFLDAVPSAGIFRETVTKVNGSTV-EPIQSPSSH 376
            VG+SIGLCFFLGNAVAGALYVLGAVETFL A P+AGIFRET+TKVNG+ V E IQSP+SH
Sbjct: 241  VGISIGLCFFLGNAVAGALYVLGAVETFLKAFPAAGIFRETITKVNGTAVSESIQSPNSH 300

Query: 377  DLQVYGIIVTIFLCFIVFGGVKMINRVAPAFLIPVLFSVVCIFLGIFLAKKNDPADGVTG 436
            DLQVYGI+VTI LCFIVFGGVKMINRVAPAFL+PVL S+ CIF+GIFLAK +DP +G+TG
Sbjct: 301  DLQVYGIVVTILLCFIVFGGVKMINRVAPAFLVPVLLSIFCIFIGIFLAKTDDPDNGITG 360

Query: 437  LSMDTFKENWSSDYQMTNSAGIPDPQGNVYWNFNALVGLFFPAVTGIMAGSNRSASLKDT 496
            L + +FK+NW S YQMTN AGIPDP G  YW+FN LVGLFFPAVTGIMAGSNRSASLKDT
Sbjct: 361  LRLKSFKDNWGSAYQMTNDAGIPDPTGGTYWSFNELVGLFFPAVTGIMAGSNRSASLKDT 420

Query: 497  QRSIPMGTLFATLLTTILYLVSVLLFGALATRKRLLTDRLFTATVAWPFPAIIYVGIILS 556
            Q+SIP+GTL ATL TT LYL+SVL FGA+ATR +LLTDRL TAT+AWPFPAI++VGIILS
Sbjct: 421  QKSIPVGTLAATLTTTSLYLISVLFFGAVATRDKLLTDRLLTATIAWPFPAIVHVGIILS 480

Query: 557  TLGAALQSLTGAPRLLAAIANDNILPILNYFKVEDGCEPYFATLFTAILCIGCVIIGNLD 616
            TLGAALQSLTGAPRLLAAIAND+ILPILNYFKV D  EP+ ATLFTA +CIGCV+IGNLD
Sbjct: 481  TLGAALQSLTGAPRLLAAIANDDILPILNYFKVADTSEPHIATLFTAFICIGCVVIGNLD 540

Query: 617  LITPTVTMFFLLCYAGVNLSCFLLDLLDAPSWRPRWKFHHWSLSLLGASLCVVIMFLISW 676
            LITPTVTMF+LLCY+GVNLSCFLLDLLDAPSWRPRWK+HHWSLS +GASLC+VIMFLISW
Sbjct: 541  LITPTVTMFYLLCYSGVNLSCFLLDLLDAPSWRPRWKYHHWSLSFVGASLCIVIMFLISW 600

Query: 677  SFTIVSLALASLIYYYVCLRGKAGDWGDGFKSAYFQLALRSLRSLGASQVHPKNWYPIPL 736
            SFT+V++ALASLIY YV L+GKAGDWGDGFKSAYFQLALRSLRSLGA+QVHPKNWYPIPL
Sbjct: 601  SFTVVAIALASLIYKYVGLKGKAGDWGDGFKSAYFQLALRSLRSLGANQVHPKNWYPIPL 660

Query: 737  IFCRPWGKLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYHERVEDSKAACKQLAT 796
            +FCRPWG+LPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDY+E  E++K ACKQLAT
Sbjct: 661  VFCRPWGQLPENVPCHPKLADFANCMKKKGRGMSIFVSILDGDYYECAEEAKEACKQLAT 720

Query: 797  YIDYKRCEGVAEIVVAPSMSEGFRGIVQTMGLGNLKPNIIVMRYPEIWRRENLTEIPATF 856
            YI+YKRCEGVAEIVVAP+M+EGFRGI+QTMGLGNLKPNI+VMRYPEIWRRENLTEIP+TF
Sbjct: 721  YIEYKRCEGVAEIVVAPNMTEGFRGIIQTMGLGNLKPNIVVMRYPEIWRRENLTEIPSTF 780

Query: 857  VGIINDCIDANKAVVIVKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 916
            VGIINDCI ANKAVVI+KGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE
Sbjct: 781  VGIINDCITANKAVVIIKGLDEWPNEYQRQYGTIDLYWIVRDGGLMLLLSQLLLTKESFE 840

Query: 917  SCKIQVFCIAEEDSDAEALKADVKKFLYDLRMQAEVIVITIKSWDTTQVEGGQQDESIEA 976
            SCKIQ+FCIAEEDSDAEALKADVKKFLYDLRM AEVIV+T+KSWD  + EG  Q++S+EA
Sbjct: 841  SCKIQLFCIAEEDSDAEALKADVKKFLYDLRMHAEVIVVTMKSWD-IRSEGNSQEDSLEA 900

Query: 977  FTAGQGRIASYLGEMKAAAAESRATTLMADGKPVNVNEKQVQKFLNTTLKLNSSILRYSR 1036
            F A Q RI+ YLGE+K       +  L+A+GKP+ VNE+QV+KFL T LKLNS+IL YSR
Sbjct: 901  FDAAQRRISDYLGEIK----RQGSNPLLANGKPMVVNEQQVEKFLYTMLKLNSTILSYSR 960

Query: 1037 MAAVVLVSLPPPPPNHPAYFYMEYLDLLVENVPRLLIVRGYRRDVVTLYT 1079
            MAAVVLVSLPPPP NHPAYFYMEY+DLLVENVPR+LIVRGY RDVVTL+T
Sbjct: 961  MAAVVLVSLPPPPLNHPAYFYMEYMDLLVENVPRMLIVRGYHRDVVTLFT 975