Cp4.1LG09g06990 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g06990 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 1.6e-58
Identity = 160/554 (28.88%), Postives = 296/554 (53.43%), Query Frame = 0

Query: 431  TAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAALSKPP 490
            T++ ++     + + +   I+++C+S+ L +CE AV  + +   D+  +  I + LSKP 
Sbjct: 645  TSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPT 704

Query: 491  VVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFL 550
            ++ G++E+L AS + E+L   I +L+EL   ++ + + + S D        LLK+     
Sbjct: 705  IINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LA 764

Query: 551  KASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGF 610
            +A++++Y  +P   Q+ + E +P ++ V++   +    F +   P +AA  +L+Q L G 
Sbjct: 765  EAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGG 824

Query: 611  DEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNK 670
            DE     N   +I+  G+  L++ LE   +E R++ VS+++CC++A+ SC+N +A  +  
Sbjct: 825  DEYSRSLNASSVISANGIPTLVKYLE--RMEGRRSVVSVLLCCMQAEKSCKNLIANRIEL 884

Query: 671  ASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLSIMNVLSIYLQ 730
            + +LEL  H  N +     +  L +L+ L+RRT   +LL  +KD     S M+   +YLQ
Sbjct: 885  SPVLELF-HSGNDSVRGTCVEFLSELVQLNRRTSCNQLLHTIKDE-GAFSTMHTFLVYLQ 944

Query: 731  RARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKSQANLARALLI 790
             A  E Q  VA++LLQLD + +P   +I+REEA+ T+I +L  Q     +Q     ALL 
Sbjct: 945  MAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEAL-WQKDFSNTQMKALDALLF 1004

Query: 791  LGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHM---YDDFVQSYEEEEEVVN-WQLK 850
            L G  S +G+   E WLL+ AGF +        + +    +D +++ E+E+  +N WQ +
Sbjct: 1005 LIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKR 1064

Query: 851  AATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILV 910
             A+V  NH + S+  AL   + S    +AK+CLV+ +W+++ L+ + D  +  +A   L+
Sbjct: 1065 IASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLL 1124

Query: 911  PPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVTWTAEEL 970
              +IK L+  K +ED +L + SL           + R++ +     LR L   +  A ++
Sbjct: 1125 EEVIKVLHSSKSLEDMILVTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADI 1184

Query: 971  ISIVESGSTHQYTE 981
            +  + + ++   TE
Sbjct: 1185 LKALLNLNSVDVTE 1191

BLAST of Cp4.1LG09g06990 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 7.8e-58
Identity = 168/588 (28.57%), Postives = 308/588 (52.38%), Query Frame = 0

Query: 397  TDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSS 456
            TDS  +  K DY      +      +  S   + T++ ++     + + ++  I ++C+S
Sbjct: 619  TDSPSQKYKDDYI-----RQRNNRFTRVSVGASPTSVLSQAAVETIINSLTPYITSLCTS 678

Query: 457  DILNDCEIAVRVVTKAWLDAHGDAAIEAALSKPPVVEGMLEVLLASNDDEILELVISVLA 516
            + L DCE AV  + + W D+  D  I + LSKP VV G++E+L AS + E+L   I +L+
Sbjct: 679  ENLQDCEQAVLEIARLWKDSKTDPQIHSYLSKPTVVSGLVEILSASLNREVLRRSIYILS 738

Query: 517  ELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVL 576
            EL   +E + + + S D        LLK+     +A++++Y  +P   Q+   E +P ++
Sbjct: 739  ELIFSDERVGETLNSVDSDFDCLAMLLKNG--LAEAALLIYQLRPVFAQLSEHELIPSLI 798

Query: 577  RVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLE 636
            +V++   +    F +   P  AA  +L+Q+L G DE     N   +I+  G+  +++ L+
Sbjct: 799  QVIQNKSEDIDDFQLAIDPKAAAIAILEQILIGGDEYNRSVNASSVISANGIPAIVKYLD 858

Query: 637  EGEIEERKNSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLALLVDL 696
              + E R+  +SI++CC++A+ SC++ +A  +  + +LEL  H  N +     +  L +L
Sbjct: 859  --KTEGRRPVISILLCCMQAEKSCKSSIANRIELSPVLELF-HAGNDSVRGICVEFLSEL 918

Query: 697  LCLSRRTRITRLLDGLKDGWNGLSIMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNC 756
            + L+RRT   + L  +KD     S M+   +YLQ A  E Q  VA++LLQLD + +P   
Sbjct: 919  VRLNRRTSSNQTLQIIKDE-GAFSTMHTFLVYLQMAPMEHQIAVASLLLQLDLLAEPRKM 978

Query: 757  TIFREEAIVTIITSLNAQISRDKSQANLARALLILGGRFSCTGEPTIENWLLQQAGFKET 816
            +I+REEA+ T+I +L  Q     +Q     ALL L G  + +G+   E  LL+ AGF + 
Sbjct: 979  SIYREEAVETLIEAL-WQKDFSNNQMKALDALLFLIGHVTSSGKSYTEAGLLKIAGFDQP 1038

Query: 817  SGDSFHSK---HMYDDFVQSYEEEEEVV-NWQLKAATVFFNHGHKSLLSALSTSMTSCIP 876
                  ++   H  +DF+++ E+E+  + +WQ + A+V  NH + S+  AL   + S   
Sbjct: 1039 YNVLMKAEQLGHSDNDFMETMEDEKNAMKSWQKRVASVLCNHENGSIFQALEECLKSNSL 1098

Query: 877  SLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKDVEDRVLASYSLLNL 936
             +AK+CLV+ +W++  LF + D  +  +A   L+  L+  L   K++E+++LAS +L + 
Sbjct: 1099 KMAKSCLVLATWLTHMLFTLPDTGVRDVARKSLLEALMNVLQSSKNLEEKILASLALKSF 1158

Query: 937  SKYTECKHIFRLFDEEALDHLRNLSLVTWTAEELISIVESGSTHQYTE 981
                    + R++ +     LR L   +  A +++  + + ++   TE
Sbjct: 1159 ISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADILKALLNLNSVDVTE 1194

BLAST of Cp4.1LG09g06990 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 226.1 bits (575), Expect = 1.7e-57
Identity = 157/554 (28.34%), Postives = 295/554 (53.25%), Query Frame = 0

Query: 431  TAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAALSKPP 490
            T++ ++     + + +   I+++C+S+ L +CE AV  + +   D+  +  I + LSKP 
Sbjct: 645  TSVLSQAAVETIVNSLKPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPT 704

Query: 491  VVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFL 550
            ++ G++E+L AS + E+L   I +L+EL   ++ + + + S D        LLK+     
Sbjct: 705  IINGLVEILSASRNREVLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNG--LA 764

Query: 551  KASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGF 610
            +A++++Y  +P   Q+ + E +P ++ V++   +    F +   P +AA  +L+Q L G 
Sbjct: 765  EAALLIYQLRPVFAQLSAHELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGG 824

Query: 611  DEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNK 670
            DE     N   +I+  G+  L++ LE   +E R++ VS+++CC++A+ SC+N +A  +  
Sbjct: 825  DEYSRSLNASSVISANGIPTLVKYLE--RMEGRRSVVSVLLCCMQAEKSCKNLIANRIEL 884

Query: 671  ASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLSIMNVLSIYLQ 730
            + +LEL  H  N +     +  L +L+ L+RRT   ++L  +KD     S M+   +YLQ
Sbjct: 885  SPVLELF-HSGNDSVRGTCVEFLSELVQLNRRTSCNQILHTIKDE-GAFSTMHTFLVYLQ 944

Query: 731  RARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKSQANLARALLI 790
             A  E Q  VA++LLQLD + +P   +I+REEA+ T+I +L  Q     +Q     ALL 
Sbjct: 945  MAPMEHQLAVASLLLQLDLLAEPRKMSIYREEAVETLIEAL-WQKDFSNTQMKALDALLF 1004

Query: 791  LGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHM---YDDFVQSYEEEEEVVN-WQLK 850
            L G  S +G+   E WLL+ AGF +        + +    +D +++ E+E+  +N WQ +
Sbjct: 1005 LIGHISSSGKSYTEAWLLKIAGFDQPYNALMKVEQLGQHDNDLIETMEDEKNALNSWQKR 1064

Query: 851  AATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILV 910
             A+V  NH + S+  AL   + S    +AK+CLV+ +W++  L+ + D  +  +A   L+
Sbjct: 1065 IASVLCNHENGSIFKALEECLKSNSLKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLL 1124

Query: 911  PPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVTWTAEEL 970
              +I  L   K++E+++LA+ +L             R++ +     LR L   +  A ++
Sbjct: 1125 EEVINVLQSSKNLEEKILATLALKTFISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDI 1184

Query: 971  ISIVESGSTHQYTE 981
            + ++ +  +   TE
Sbjct: 1185 MKVILNLKSVDVTE 1191

BLAST of Cp4.1LG09g06990 vs. NCBI nr
Match: XP_023542467.1 (putative E3 ubiquitin-protein ligase LIN [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1896 bits (4912), Expect = 0.0
Identity = 985/985 (100.00%), Postives = 985/985 (100.00%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780
           IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS
Sbjct: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780

Query: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840
           QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV
Sbjct: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840

Query: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900
           VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM
Sbjct: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900

Query: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960
           APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT
Sbjct: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960

Query: 961 WTAEELISIVESGSTHQYTETSSRT 985
           WTAEELISIVESGSTHQYTETSSRT
Sbjct: 961 WTAEELISIVESGSTHQYTETSSRT 985

BLAST of Cp4.1LG09g06990 vs. NCBI nr
Match: XP_022954717.1 (putative E3 ubiquitin-protein ligase LIN [Cucurbita moschata])

HSP 1 Score: 1846 bits (4782), Expect = 0.0
Identity = 963/986 (97.67%), Postives = 974/986 (98.78%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRKP RPNGGGGGRRRRRRTVSDGSVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPLRPNGGGGGRRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNE+ELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEQELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFART+LLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTYLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYY+
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYV 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAKAPPVPVVSLPSKSI GMSRRSSDSYFSQSSSS NKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKAPPVPVVSLPSKSISGMSRRSSDSYFSQSSSS-NKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LRTAAKARSSNEKSVPVHDEPSNGISSSIL YKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILCYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAK AIR EGRNHNLSSVV+RAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKNAIRVEGRNHNLSSVVNRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIEA LSKP VVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEAVLSKPLVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRR RI+RLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRARISRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780
           IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNA+ISR+K+
Sbjct: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNARISREKT 780

Query: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840
           + NLARALLILGGRFSCTGEPTIENWLLQQAGFKE+SGDSFHSKHMYDDFVQSYEEEEEV
Sbjct: 781 RENLARALLILGGRFSCTGEPTIENWLLQQAGFKESSGDSFHSKHMYDDFVQSYEEEEEV 840

Query: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900
           VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM
Sbjct: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900

Query: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960
           APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEAL+HLRNLSLVT
Sbjct: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALEHLRNLSLVT 960

Query: 961 WTAEELISIVE-SGSTHQYTETSSRT 985
           WTAEELISIVE SGSTHQYTETSSRT
Sbjct: 961 WTAEELISIVEMSGSTHQYTETSSRT 985

BLAST of Cp4.1LG09g06990 vs. NCBI nr
Match: XP_022994727.1 (putative E3 ubiquitin-protein ligase LIN isoform X1 [Cucurbita maxima])

HSP 1 Score: 1845 bits (4779), Expect = 0.0
Identity = 960/985 (97.46%), Postives = 971/985 (98.58%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRKPSRPNGGGG RRRRRRTVSDGSVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGG-RRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVS+LSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSILSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSS NKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSS-NKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LR+AAKARSSNE SVPVHDE SNGI+SSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRSAAKARSSNESSVPVHDELSNGITSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAK AIR EGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKNAIRVEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIE ALSKPPV+EGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEVALSKPPVIEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRC PHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCMPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERK SVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKKSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRI+RLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRISRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780
           IMNVLS+YLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNA+IS +K+
Sbjct: 721 IMNVLSVYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNARISHEKT 780

Query: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840
           Q NLARALLILGGRFSCTGEPTIENWLLQQAGFKE SG SFHSKHMYDDFVQSYEEEEEV
Sbjct: 781 QENLARALLILGGRFSCTGEPTIENWLLQQAGFKENSGGSFHSKHMYDDFVQSYEEEEEV 840

Query: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900
           VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM
Sbjct: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900

Query: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960
           APSILVPPLIKYLNYDK+VED+VLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT
Sbjct: 901 APSILVPPLIKYLNYDKEVEDQVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960

Query: 961 WTAEELISIVESGSTHQYTETSSRT 985
           WTAEELISIVESGSTHQYTETSSRT
Sbjct: 961 WTAEELISIVESGSTHQYTETSSRT 983

BLAST of Cp4.1LG09g06990 vs. NCBI nr
Match: KAG6573444.1 (Protein ROOT HAIR DEFECTIVE 3-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1841 bits (4769), Expect = 0.0
Identity = 954/978 (97.55%), Postives = 964/978 (98.57%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRK SRPNGGGGGR RRRRTVSDGSVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKSSRPNGGGGGRGRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNE+ELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEQELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAK PPVPVVSLPSKSI G SRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKGPPVPVVSLPSKSICGRSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LRTAAKARSSNE SVPVHDEPSNGI+SS+LRYKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRTAAKARSSNESSVPVHDEPSNGITSSVLRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAK  IR EGRNHNLSSVV+RAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKNTIRVEGRNHNLSSVVNRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRR RI+RLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRARISRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780
           IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNA+ISR+K+
Sbjct: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNARISREKT 780

Query: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840
           Q NLARALLILGGRFSCTGEPTIENWLLQQAGFKE+SGDSFHSKHMYDDFVQSYEEEEEV
Sbjct: 781 QENLARALLILGGRFSCTGEPTIENWLLQQAGFKESSGDSFHSKHMYDDFVQSYEEEEEV 840

Query: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900
           V WQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM
Sbjct: 841 VTWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900

Query: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960
           APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT
Sbjct: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960

Query: 961 WTAEELISIVESGSTHQY 978
           WTAEELISIVESGSTH +
Sbjct: 961 WTAEELISIVESGSTHHF 978

BLAST of Cp4.1LG09g06990 vs. NCBI nr
Match: XP_038894766.1 (putative E3 ubiquitin-protein ligase LIN isoform X1 [Benincasa hispida])

HSP 1 Score: 1514 bits (3921), Expect = 0.0
Identity = 805/1009 (79.78%), Postives = 873/1009 (86.52%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEG NF   RK  R  G       R R   D SVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGSNFPTTRKSLRSKG-------RSRAAPDDSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKK +K L+RNGSSVYSSKRVGSVSET LCKSME     EPAID IAIRAVVS+LSGY
Sbjct: 61  SSKKKAEKPLIRNGSSVYSSKRVGSVSETLLCKSME-----EPAIDEIAIRAVVSILSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEKELRVK 180
           VGRY KDESFRE++RKKC  C         GIC NLEMGMKSVDRLVEE  GNE+ELR+K
Sbjct: 121 VGRYSKDESFREIMRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGQGNERELRMK 180

Query: 181 ASRNSIGLLNMVI----SATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLF 240
           ASRNSIGLLNMVI    SA K+ KNG +SHLS+CA LYLAIVYKIEKNEKVSAKH+LQ+F
Sbjct: 181 ASRNSIGLLNMVITSLNSAKKSTKNGAQSHLSSCAQLYLAIVYKIEKNEKVSAKHLLQVF 240

Query: 241 CDYPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEY 300
           CD PFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSN + EHK+R IKALSKVYNE+
Sbjct: 241 CDSPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALSKVYNEH 300

Query: 301 MDMGTAQFAVYYIQWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYH 360
           MD GT QFA+YYIQWLKDGA+APPVPVV  PSKSIH  SRRSSDSYF QSSS  NKNLYH
Sbjct: 301 MDRGTVQFALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFLQSSS--NKNLYH 360

Query: 361 AVFGPSLDQQLAELRT----AAKARSSNEKSV-----------PVHDEPSNGISSSILRY 420
           AVFGPSLDQQLAELR+    AAKARSSNEK +            V DE +N    SIL Y
Sbjct: 361 AVFGPSLDQQLAELRSGNVVAAKARSSNEKEILYSNKNYENIASVRDEHNNRRMPSILDY 420

Query: 421 KTHNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKTAI-RAEGRNHNLSSVVSRAIA 480
           + HNTDSWRETVKSDYF FFTCQNITKE+ ESSN++ K ++ R EGRNH LS+  S+AI 
Sbjct: 421 RGHNTDSWRETVKSDYFRFFTCQNITKEYLESSNVITKNSVVRVEGRNHLLSNDFSKAIT 480

Query: 481 TICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAALSKPPVVEGMLEVLLASNDDEILELV 540
            ICSSD+L++CEIA+RVVTKAWLDAH D  IE ALSKPPVVEGMLEVLLAS+DDEILELV
Sbjct: 481 AICSSDVLSECEIAIRVVTKAWLDAHDDTTIEVALSKPPVVEGMLEVLLASDDDEILELV 540

Query: 541 ISVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEW 600
           ISVLAELAA++E+IRQIIL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEW
Sbjct: 541 ISVLAELAARSEVIRQIILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEW 600

Query: 601 LPLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLL 660
           LPLVLRVLEFGGQLQTLFS+RC PHEAAFYLLDQLLKGFDEDRNLENCRHLI+LGGLSLL
Sbjct: 601 LPLVLRVLEFGGQLQTLFSIRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLISLGGLSLL 660

Query: 661 LRRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLA 720
           LRRLE GEIEERKNSVSII CCI+ADGSCRNYLAEN+NKASLLELIVHESN NSDRCGLA
Sbjct: 661 LRRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELIVHESNKNSDRCGLA 720

Query: 721 LLVDLLCLSRRTRITRLLDGLKDGWNGLSIMNVLSIYLQRARPEEQPLVATILLQLDFME 780
           LLVDLLCLSRRTRIT LLDGLK+GW+GL IMN+LS+YLQRA PEEQPLVATILLQLDFME
Sbjct: 721 LLVDLLCLSRRTRITNLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATILLQLDFME 780

Query: 781 DPLNCTIFREEAIVTIITSLNAQISRDKSQANLARALLILGGRFSCTGEPTIENWLLQQA 840
           D LNC+IFREEAIVTIIT+LN++I R+K Q NLARALLILGGRFSCTGEP+ ENWLL+ A
Sbjct: 781 DSLNCSIFREEAIVTIITALNSRICREKGQDNLARALLILGGRFSCTGEPSTENWLLKLA 840

Query: 841 GFKETSGDSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCI 900
           GFKE SGDS HS+ +YDD VQ YEEEE+VVNWQLKAATV FNHGHKSLLSALST MTSCI
Sbjct: 841 GFKENSGDSSHSQPLYDDVVQLYEEEEDVVNWQLKAATVLFNHGHKSLLSALSTLMTSCI 900

Query: 901 PSLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKDVEDRVLASYSLLN 960
            SLAKA L+ VSWMS+YLFV+ D+KLCLMAPSILVPPLIKYLN+DKDVED+VLASYSLLN
Sbjct: 901 LSLAKASLITVSWMSRYLFVIKDEKLCLMAPSILVPPLIKYLNHDKDVEDQVLASYSLLN 960

Query: 961 LSKYTECKHIFRLFDEEALDHLRNLSLVTWTAEELISIVESGSTHQYTE 980
           LSKYTECKHIFRLFDE+ LD+L+NLSLVTWTAEEL  I++S S H YTE
Sbjct: 961 LSKYTECKHIFRLFDEDTLDYLQNLSLVTWTAEELTLILKSRSMHPYTE 995

BLAST of Cp4.1LG09g06990 vs. ExPASy TrEMBL
Match: A0A6J1GRP8 (putative E3 ubiquitin-protein ligase LIN OS=Cucurbita moschata OX=3662 GN=LOC111456892 PE=4 SV=1)

HSP 1 Score: 1846 bits (4782), Expect = 0.0
Identity = 963/986 (97.67%), Postives = 974/986 (98.78%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRKP RPNGGGGGRRRRRRTVSDGSVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPLRPNGGGGGRRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNE+ELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEQELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFART+LLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTYLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYY+
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYV 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAKAPPVPVVSLPSKSI GMSRRSSDSYFSQSSSS NKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKAPPVPVVSLPSKSISGMSRRSSDSYFSQSSSS-NKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LRTAAKARSSNEKSVPVHDEPSNGISSSIL YKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILCYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAK AIR EGRNHNLSSVV+RAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKNAIRVEGRNHNLSSVVNRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIEA LSKP VVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEAVLSKPLVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRR RI+RLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRARISRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780
           IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNA+ISR+K+
Sbjct: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNARISREKT 780

Query: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840
           + NLARALLILGGRFSCTGEPTIENWLLQQAGFKE+SGDSFHSKHMYDDFVQSYEEEEEV
Sbjct: 781 RENLARALLILGGRFSCTGEPTIENWLLQQAGFKESSGDSFHSKHMYDDFVQSYEEEEEV 840

Query: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900
           VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM
Sbjct: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900

Query: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960
           APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEAL+HLRNLSLVT
Sbjct: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALEHLRNLSLVT 960

Query: 961 WTAEELISIVE-SGSTHQYTETSSRT 985
           WTAEELISIVE SGSTHQYTETSSRT
Sbjct: 961 WTAEELISIVEMSGSTHQYTETSSRT 985

BLAST of Cp4.1LG09g06990 vs. ExPASy TrEMBL
Match: A0A6J1JWN9 (putative E3 ubiquitin-protein ligase LIN isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490369 PE=4 SV=1)

HSP 1 Score: 1845 bits (4779), Expect = 0.0
Identity = 960/985 (97.46%), Postives = 971/985 (98.58%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRKPSRPNGGGG RRRRRRTVSDGSVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGG-RRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVS+LSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSILSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSS NKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSS-NKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LR+AAKARSSNE SVPVHDE SNGI+SSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRSAAKARSSNESSVPVHDELSNGITSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAK AIR EGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKNAIRVEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIE ALSKPPV+EGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEVALSKPPVIEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRC PHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCMPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERK SVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKKSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRI+RLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRISRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNAQISRDKS 780
           IMNVLS+YLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNA+IS +K+
Sbjct: 721 IMNVLSVYLQRARPEEQPLVATILLQLDFMEDPLNCTIFREEAIVTIITSLNARISHEKT 780

Query: 781 QANLARALLILGGRFSCTGEPTIENWLLQQAGFKETSGDSFHSKHMYDDFVQSYEEEEEV 840
           Q NLARALLILGGRFSCTGEPTIENWLLQQAGFKE SG SFHSKHMYDDFVQSYEEEEEV
Sbjct: 781 QENLARALLILGGRFSCTGEPTIENWLLQQAGFKENSGGSFHSKHMYDDFVQSYEEEEEV 840

Query: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900
           VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM
Sbjct: 841 VNWQLKAATVFFNHGHKSLLSALSTSMTSCIPSLAKACLVMVSWMSKYLFVVTDDKLCLM 900

Query: 901 APSILVPPLIKYLNYDKDVEDRVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960
           APSILVPPLIKYLNYDK+VED+VLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT
Sbjct: 901 APSILVPPLIKYLNYDKEVEDQVLASYSLLNLSKYTECKHIFRLFDEEALDHLRNLSLVT 960

Query: 961 WTAEELISIVESGSTHQYTETSSRT 985
           WTAEELISIVESGSTHQYTETSSRT
Sbjct: 961 WTAEELISIVESGSTHQYTETSSRT 983

BLAST of Cp4.1LG09g06990 vs. ExPASy TrEMBL
Match: A0A6J1CLV0 (putative E3 ubiquitin-protein ligase LIN-1 OS=Momordica charantia OX=3673 GN=LOC111012647 PE=4 SV=1)

HSP 1 Score: 1502 bits (3889), Expect = 0.0
Identity = 797/1008 (79.07%), Postives = 878/1008 (87.10%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEG N+   RKPSRP G       RRRT +DGSVTLPIYICHD K ID
Sbjct: 1   MASLQELLTREGFEGTNYPKTRKPSRPRG-------RRRTPADGSVTLPIYICHDKKFID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKK DKA VRNGSSVYSSKRVGSVSE+SLCKSME     EPAID IAIRAVVS+LSGY
Sbjct: 61  SSKKKADKAFVRNGSSVYSSKRVGSVSESSLCKSME-----EPAIDDIAIRAVVSILSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEKELRVK 180
           VG Y KDE FRE VRKKC  C         GIC NLEMGMKSVDRLVEE +GNEKELRVK
Sbjct: 121 VGSYAKDEIFRETVRKKCNLCLVRSGEMGVGICSNLEMGMKSVDRLVEEGHGNEKELRVK 180

Query: 181 ASRNSIGLLNMVISA---TKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFC 240
           ASRNSIGLLNMV+++    ++M+NG+ S LSACA LYLAIVYKIEKNEK+SAKH+LQ+FC
Sbjct: 181 ASRNSIGLLNMVVASLNSVRSMENGS-SDLSACAKLYLAIVYKIEKNEKISAKHLLQVFC 240

Query: 241 DYPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYM 300
           D PF ARTHLLPELWEHFFLPHLLHLKVWY+QELEFVSN++ EHK+R IKALSKVYNE+M
Sbjct: 241 DSPFLARTHLLPELWEHFFLPHLLHLKVWYSQELEFVSNVECEHKDRRIKALSKVYNEHM 300

Query: 301 DMGTAQFAVYYIQWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHA 360
           D GT QFA+YYIQWLKDGAKAPPVPVV  PSKSIHG SRRSSDSYFSQSSS  NKNLYHA
Sbjct: 301 DRGTVQFALYYIQWLKDGAKAPPVPVVPSPSKSIHGASRRSSDSYFSQSSS--NKNLYHA 360

Query: 361 VFGPSLDQQLAELRT----AAKARSSNEK-----------SVPVHDEPSNGISSSILRYK 420
           VFGPSL++Q+ ELR+    AAK RSSNEK           S  VH+E SN   SS+L Y+
Sbjct: 361 VFGPSLEEQVIELRSGNMAAAKTRSSNEKEILFAEKNYESSASVHEELSNHRMSSVLDYR 420

Query: 421 THNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAK-TAIRAEGRNHNLSSVVSRAIAT 480
             N DSWRETVKSDY  FFTCQN+ KE+ E+SN++ + +A+R +G++H LS+ +SRAI+T
Sbjct: 421 HQNADSWRETVKSDYLRFFTCQNVAKEYLENSNVITRNSAVRVDGKSHLLSNDLSRAIST 480

Query: 481 ICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAALSKPPVVEGMLEVLLASNDDEILELVI 540
           ICSSDIL++CEIA+RVVTKAWLDAHGD AIE ALSKPPVVEGMLEVLLAS+DDEILELVI
Sbjct: 481 ICSSDILSECEIAIRVVTKAWLDAHGDTAIEVALSKPPVVEGMLEVLLASDDDEILELVI 540

Query: 541 SVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEWL 600
           SVLAELAA++E+IRQ+IL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEWL
Sbjct: 541 SVLAELAARDEVIRQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEWL 600

Query: 601 PLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL 660
           PLVLRVLEFGGQLQTLFSVRC PHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL
Sbjct: 601 PLVLRVLEFGGQLQTLFSVRCKPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLLL 660

Query: 661 RRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLAL 720
           RRLE GEIEERKN+VSII CCI+ADGSCRNYL +N+NK SLLELIVHESN +SDRCGLAL
Sbjct: 661 RRLERGEIEERKNAVSIISCCIQADGSCRNYLVDNLNKTSLLELIVHESNKSSDRCGLAL 720

Query: 721 LVDLLCLSRRTRITRLLDGLKDGWNGLSIMNVLSIYLQRARPEEQPLVATILLQLDFMED 780
           LVDLLCLSRRTRITRLLDGLK+GW+GL IMN+LS+YLQRA PEEQPLVA ILLQLDFMED
Sbjct: 721 LVDLLCLSRRTRITRLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVAAILLQLDFMED 780

Query: 781 PLNCTIFREEAIVTIITSLNAQISRDKSQANLARALLILGGRFSCTGEPTIENWLLQQAG 840
             NC+I+REEAIVTIIT+L ++ISR++ Q NLARALLILGGRF  TGEP+ ENWLL +AG
Sbjct: 781 TQNCSIYREEAIVTIITALKSRISREEVQENLARALLILGGRFLYTGEPSAENWLLLRAG 840

Query: 841 FKETSGDSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCIP 900
           FKE+S DSFHSKH+YDDFVQSYEEEE+V NWQLKAATV FNHGHKSLLSALS S++S IP
Sbjct: 841 FKESSEDSFHSKHVYDDFVQSYEEEEDVENWQLKAATVLFNHGHKSLLSALSISLSSSIP 900

Query: 901 SLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKDVEDRVLASYSLLNL 960
           SLAKA LV VSWMS YLFVV D+KL L+ PSILVPPLIKYLNYDKDVEDRVLASYSLLNL
Sbjct: 901 SLAKASLVTVSWMSTYLFVVRDEKLQLITPSILVPPLIKYLNYDKDVEDRVLASYSLLNL 960

Query: 961 SKYTECKHIFRLFDEEALDHLRNLSLVTWTAEELISIVESGSTHQYTE 980
           SKYTECKHIFRLFDEE LD L+NLSLVTWTAEEL+ IV SGS HQYT+
Sbjct: 961 SKYTECKHIFRLFDEEVLDRLQNLSLVTWTAEELVLIVRSGSAHQYTK 993

BLAST of Cp4.1LG09g06990 vs. ExPASy TrEMBL
Match: A0A0A0LYR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G713660 PE=4 SV=1)

HSP 1 Score: 1501 bits (3887), Expect = 0.0
Identity = 800/1010 (79.21%), Postives = 876/1010 (86.73%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEG N+ + RK SRP G       R RT  D SVTLPIYICHD K ID
Sbjct: 1   MASLQELLTREGFEGSNYPSTRKLSRPKG-------RSRTAPDDSVTLPIYICHDKKTID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKK DK LVRNGSSVYSSKRVGSVSET  CKSME     EPAID IAIRAVVS+LSGY
Sbjct: 61  SSKKKHDKPLVRNGSSVYSSKRVGSVSETLPCKSME-----EPAIDEIAIRAVVSILSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSC---------GICGNLEMGMKSVDRLVEERYGNEKELRVK 180
           VGRY KDE+FRE+VRKKC  C         GIC NLEMGMKSVDRLVEE +GNE+ELR+K
Sbjct: 121 VGRYSKDENFREIVRKKCNPCLIRKGEMESGICSNLEMGMKSVDRLVEEGHGNERELRIK 180

Query: 181 ASRNSIGLLNMVI----SATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLF 240
           ASRNSIGLLNMVI    SA  + KNG  SHLSACA LYLAIV KIEKNE+VSAKH+LQ+F
Sbjct: 181 ASRNSIGLLNMVITSLDSAKNSTKNGAHSHLSACAQLYLAIVNKIEKNERVSAKHLLQVF 240

Query: 241 CDYPFFARTHLLPELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEY 300
           CD P+FARTHLLP+LWEHFFLPHLLHLKVWYNQELEFVSN + EHK+R IKAL+KVYN++
Sbjct: 241 CDSPYFARTHLLPDLWEHFFLPHLLHLKVWYNQELEFVSNFECEHKDRKIKALNKVYNDH 300

Query: 301 MDMGTAQFAVYYIQWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYH 360
           MD GT QFA+YYIQWLKDGA+APPVPVV  PSKSIH  SRRSSDSYFSQ SS  NKNLYH
Sbjct: 301 MDRGTVQFALYYIQWLKDGARAPPVPVVPSPSKSIHKASRRSSDSYFSQPSS--NKNLYH 360

Query: 361 AVFGPSLDQQLAELR----TAAKARSSNEK-----------SVPVHDEPSNGISSSILRY 420
           AVFGPSLDQQLAELR     AA ARSS+EK           S  V DE SN   SS+L Y
Sbjct: 361 AVFGPSLDQQLAELRRGNLVAAMARSSHEKEILFSDKHYENSASVQDEHSNRRMSSVLDY 420

Query: 421 KTHNTDSWRETVKSDYFSFFTCQNITKEHSESSNIVAKTA-IRAEGRNHNLSSVVSRAIA 480
           ++HNT+SWRETVKSDYF FFTCQNITKE+ ESSN++ K + ++ EGRNH LS+  S+AI 
Sbjct: 421 RSHNTESWRETVKSDYFRFFTCQNITKEYLESSNVITKNSFVKVEGRNHLLSNDFSKAIT 480

Query: 481 TICSSDILNDCEIAVRVVTKAWLDAHGDAAIEAALSKPPVVEGMLEVLLASNDDEILELV 540
            ICSSDIL++CEIAVRVVTKAWLDAHGD+ IE ALS+PPVVEGMLEVLLAS+DDEILELV
Sbjct: 481 AICSSDILSECEIAVRVVTKAWLDAHGDSNIEVALSQPPVVEGMLEVLLASDDDEILELV 540

Query: 541 ISVLAELAAKNEIIRQIILSSDPQLQVFLKLLKSSSLFLKASIVLYLSKPQAKQMVSVEW 600
           ISVLAELAA++EII+Q+IL+SDPQLQVFLKLLKSSSLFLKASI+LYLSKPQAKQM+SVEW
Sbjct: 541 ISVLAELAARSEIIQQMILNSDPQLQVFLKLLKSSSLFLKASILLYLSKPQAKQMISVEW 600

Query: 601 LPLVLRVLEFGGQLQTLFSVRCTPHEAAFYLLDQLLKGFDEDRNLENCRHLIALGGLSLL 660
           LPLVLRVLEFGGQLQTLFSV+C PHEAAFYLLDQLLKGFDEDRNLEN RHLIALGGLSLL
Sbjct: 601 LPLVLRVLEFGGQLQTLFSVQCKPHEAAFYLLDQLLKGFDEDRNLENSRHLIALGGLSLL 660

Query: 661 LRRLEEGEIEERKNSVSIIMCCIRADGSCRNYLAENMNKASLLELIVHESNTNSDRCGLA 720
           LRRLE GEIEERKNSVSII CCI+ADGSCRNYLAEN+NKASLLEL+VHESN NSDR GLA
Sbjct: 661 LRRLERGEIEERKNSVSIISCCIQADGSCRNYLAENLNKASLLELVVHESNKNSDRGGLA 720

Query: 721 LLVDLLCLSRRTRITRLLDGLKDGWNGLSIMNVLSIYLQRARPEEQPLVATILLQLDFME 780
           LLVDLLCLSRRTRIT+LLDGLK+GW+GL IMN+LS+YLQRA PEEQPLVAT LLQLDFME
Sbjct: 721 LLVDLLCLSRRTRITKLLDGLKEGWSGLGIMNILSVYLQRALPEEQPLVATTLLQLDFME 780

Query: 781 DPLNCTIFREEAIVTIITSLNAQISRDKSQANLARALLILGGRFSCTGEPTIENWLLQQA 840
           D LNC+IFREEAIVTIIT+LNA+I  +K+Q NLARALLILGGRFSCTGEP+ ENWLL+ A
Sbjct: 781 DALNCSIFREEAIVTIITALNARICGEKAQDNLARALLILGGRFSCTGEPSTENWLLKLA 840

Query: 841 GFKETSGDSFHSKHMYDDFVQSYEEEEEVVNWQLKAATVFFNHGHKSLLSALSTSMTSCI 900
           GFKE SGDS HSKH+YDD VQ YEEEEEV+NWQLKAA+V FNHGHKSLLS+LSTSMTSCI
Sbjct: 841 GFKENSGDSSHSKHLYDDVVQLYEEEEEVMNWQLKAASVLFNHGHKSLLSSLSTSMTSCI 900

Query: 901 -PSLAKACLVMVSWMSKYLFVVTDDKLCLMAPSILVPPLIKYLNYDKDVEDRVLASYSLL 960
            PSLAKACL+ +SWMS+YLFV+ ++KLCLMAPSILVPPLIKYLN+DK VED+VLASYSLL
Sbjct: 901 RPSLAKACLITLSWMSRYLFVIREEKLCLMAPSILVPPLIKYLNHDKAVEDQVLASYSLL 960

Query: 961 NLSKYTECKHIFRLFDEEALDHLRNLSLVTWTAEELISIVESGSTHQYTE 980
           NL KYTECKHIFRLFD+EALDHLRNLSLVTWTAEELI I+ SGS   YTE
Sbjct: 961 NLGKYTECKHIFRLFDDEALDHLRNLSLVTWTAEELILIITSGSMDPYTE 996

BLAST of Cp4.1LG09g06990 vs. ExPASy TrEMBL
Match: A0A6J1K5Y5 (putative E3 ubiquitin-protein ligase LIN isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490369 PE=4 SV=1)

HSP 1 Score: 1404 bits (3635), Expect = 0.0
Identity = 734/750 (97.87%), Postives = 740/750 (98.67%), Query Frame = 0

Query: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGGGRRRRRRTVSDGSVTLPIYICHDMKIID 60
           MASLQELLTREGFEGENFSNNRKPSRPNGGGG RRRRRRTVSDGSVTLPIYICHD KIID
Sbjct: 1   MASLQELLTREGFEGENFSNNRKPSRPNGGGG-RRRRRRTVSDGSVTLPIYICHDKKIID 60

Query: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSVLSGY 120
           SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVS+LSGY
Sbjct: 61  SSKKKLDKALVRNGSSVYSSKRVGSVSETSLCKSMEGSKVEEPAIDGIAIRAVVSILSGY 120

Query: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180
           VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL
Sbjct: 121 VGRYLKDESFREMVRKKCKSCGICGNLEMGMKSVDRLVEERYGNEKELRVKASRNSIGLL 180

Query: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240
           NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP
Sbjct: 181 NMVISATKTMKNGTRSHLSACALLYLAIVYKIEKNEKVSAKHVLQLFCDYPFFARTHLLP 240

Query: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300
           ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI
Sbjct: 241 ELWEHFFLPHLLHLKVWYNQELEFVSNLDYEHKNRTIKALSKVYNEYMDMGTAQFAVYYI 300

Query: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSSSNKNLYHAVFGPSLDQQLAE 360
           QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSS NKNLYHAVFGPSLDQQLAE
Sbjct: 301 QWLKDGAKAPPVPVVSLPSKSIHGMSRRSSDSYFSQSSSS-NKNLYHAVFGPSLDQQLAE 360

Query: 361 LRTAAKARSSNEKSVPVHDEPSNGISSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420
           LR+AAKARSSNE SVPVHDE SNGI+SSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE
Sbjct: 361 LRSAAKARSSNESSVPVHDELSNGITSSILRYKTHNTDSWRETVKSDYFSFFTCQNITKE 420

Query: 421 HSESSNIVAKTAIRAEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480
           HSESSNIVAK AIR EGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA
Sbjct: 421 HSESSNIVAKNAIRVEGRNHNLSSVVSRAIATICSSDILNDCEIAVRVVTKAWLDAHGDA 480

Query: 481 AIEAALSKPPVVEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540
           AIE ALSKPPV+EGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL
Sbjct: 481 AIEVALSKPPVIEGMLEVLLASNDDEILELVISVLAELAAKNEIIRQIILSSDPQLQVFL 540

Query: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCTPHEAAF 600
           KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRC PHEAAF
Sbjct: 541 KLLKSSSLFLKASIVLYLSKPQAKQMVSVEWLPLVLRVLEFGGQLQTLFSVRCMPHEAAF 600

Query: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKNSVSIIMCCIRADGSC 660
           YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERK SVSIIMCCIRADGSC
Sbjct: 601 YLLDQLLKGFDEDRNLENCRHLIALGGLSLLLRRLEEGEIEERKKSVSIIMCCIRADGSC 660

Query: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRITRLLDGLKDGWNGLS 720
           RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRI+RLLDGLKDGWNGLS
Sbjct: 661 RNYLAENMNKASLLELIVHESNTNSDRCGLALLVDLLCLSRRTRISRLLDGLKDGWNGLS 720

Query: 721 IMNVLSIYLQRARPEEQPLVATILLQLDFM 750
           IMNVLS+YLQRARPEEQPLVATILLQLDFM
Sbjct: 721 IMNVLSVYLQRARPEEQPLVATILLQLDFM 748