Cp4.1LG09g06240 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g06240 vs. ExPASy Swiss-Prot
Match: A1L4V7 (D-cysteine desulfhydrase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g26115 PE=2 SV=1)

HSP 1 Score: 461.1 bits (1185), Expect = 1.6e-128
Identity = 238/428 (55.61%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 71  SSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLEGSPANHLCLA 130
           SS+++  +S++      +FV++LLDR+W L  P S I ++ +    ++++G   +     
Sbjct: 23  SSQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISV----SSVKG--IDKFSFL 82

Query: 131 VDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 190
            +T     +++S++     SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 83  NNTRPHLGDEMSKSKQG-SSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 142

Query: 191 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 250
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 143 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 202

Query: 251 LKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVINEG 310
           L+T A+LVAG  G++LW  D+ +         T N   +D DF    +   RKV+++NEG
Sbjct: 203 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 262

Query: 311 AGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALCLGLPWEVTAV 370
           AGD++ALLG+ RLV +LSQDHLLGK+R +KFV+DAGTGTTA+GLG+ A+ LGLPWE+ AV
Sbjct: 263 AGDALALLGMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAAMSLGLPWEINAV 322

Query: 371 MLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVLDGE 430
           MLAD +  YKR E  LI+EF + F  P  V     ++N  ++ W++R  PRKFG VL+GE
Sbjct: 323 MLADTLKNYKRHEDHLIAEFSRQF--PGSVFCSGLDMN-QMIKWIDRQHPRKFGKVLEGE 382

Query: 431 VEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQRYK 490
           VE+C++IAQETG+LVDP+YTLAAWE AT L Q   +  + VVMLHTGGTLGMFG+AQRYK
Sbjct: 383 VEMCRKIAQETGVLVDPMYTLAAWETATELVQD--EKSSIVVMLHTGGTLGMFGLAQRYK 427

Query: 491 SYFNNLKN 499
           + F NLK+
Sbjct: 443 TCFTNLKD 427

BLAST of Cp4.1LG09g06240 vs. ExPASy Swiss-Prot
Match: B9EYZ1 (Putative D-cysteine desulfhydrase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0695600 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 7.1e-108
Identity = 209/414 (50.48%), Postives = 269/414 (64.98%), Query Frame = 0

Query: 85  GGEDFVARLLD-RRWALANPESKINKVILLTADTNLEGSPANHLCLAVDTNICTYNDLSR 144
           GG   VA LL    W L +P ++++ + +L + +    SP +H   +  T     N    
Sbjct: 9   GGGRTVANLLSATEWMLPSPATQVHTISVLPSHS--PPSPPHHFAFSNLTTAPKRNGGKG 68

Query: 145 ANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVL 204
                  F VVRDDLLHPL NGNKARKLD  LPL+     TDVVTCGGCQSAHAAATAV 
Sbjct: 69  EEEGRPRFEVVRDDLLHPLANGNKARKLDALLPLLRRRGATDVVTCGGCQSAHAAATAVH 128

Query: 205 CAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMLKTQAELVAGSSG 264
           CAE G+R H+LLRGEQP++ TGYNL+S ++GNV Y  RS+YA+R++ML   A  VAG+ G
Sbjct: 129 CAEWGMRPHILLRGEQPDIPTGYNLISLMFGNVAYASRSVYAHRDEMLYNHARKVAGTGG 188

Query: 265 SILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVINEGAGDSIALLGLIRL 324
           ++LW DD+ +   +       N   +            R+V++I EGAGD  ALLG+IRL
Sbjct: 189 TVLWADDISKEDFV---LDEDNGCEIG----------SRRVVIIKEGAGDVQALLGVIRL 248

Query: 325 VNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALCLGLPWEVTAVMLADRIDGYKRQE 384
           V YL       K   +  V+DAGTGTTA+GL LGA+CLGL W VTAVMLAD ++ YK +E
Sbjct: 249 VEYLYNLSSFHKHENVHVVVDAGTGTTAVGLALGAVCLGLHWRVTAVMLADTLERYKERE 308

Query: 385 KRLISEFKK--HFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVLDGEVEICKQIAQET 444
           K LIS+FKK  H  Y   V  GE+++   +V WVERF PR+FG VL+GE+ +C+QIAQ+T
Sbjct: 309 KSLISDFKKLCHNNYHEMV--GENDIGDSLVEWVERFSPRRFGKVLNGEIALCRQIAQQT 368

Query: 445 GILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQRYKSYFNN 496
           GIL+DP+YTLA WE A  L    ++ K  VVM+HTGGTLG  G+AQRY S+F +
Sbjct: 369 GILLDPMYTLAGWEQAVDLCVGDSRTK--VVMIHTGGTLGFCGLAQRYSSHFTS 403

BLAST of Cp4.1LG09g06240 vs. ExPASy Swiss-Prot
Match: Q9WY68 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=TM_0225 PE=3 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.9e-20
Identity = 98/341 (28.74%), Postives = 144/341 (42.23%), Query Frame = 0

Query: 150 SFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGL 209
           + YV RDDL   + +GNK RKL+  L        T V TCGG QS HA ATA +    GL
Sbjct: 25  NIYVKRDDLTELVGSGNKIRKLEYLLWEALKKGATTVFTCGGLQSNHARATAYVSRRYGL 84

Query: 210 RSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMLKTQAELVAGSSGSILWFD 269
           +  L LR +  ++L G  L+  + G                    AE+V  S       D
Sbjct: 85  KPVLFLR-KGEKVLNGNLLLDILLG--------------------AEIVEVSPEEYERID 144

Query: 270 DVFQSSIMKQPCSTTNSVPVDYDFHIRAR-KHERKVIVINEGAGDSIALLGLIRLVNYLS 329
           ++F                   D H + R K   KV VI EG  +S+   G    V  L 
Sbjct: 145 EIF-------------------DVHKKMREKKGEKVYVIPEGGSNSLGAFGYFNAV--LE 204

Query: 330 QDHLLGKQRAIKFVIDAGTGTTAIGLGLGALCLGLPWEVTAVMLADR-IDGYKRQEKRLI 389
               L  +     V   G+G T  GL  G   L     V  V +  +  D +  + KR+I
Sbjct: 205 MKDQLNLESFDAIVCAVGSGGTIAGLSAGISFLEYHVPVVGVNVTTKNSDYFVGKVKRII 264

Query: 390 SEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVLDGEVEICKQIAQETGILVDP 449
           S  +++       GL    VN  +   V+ +R   +      +VEI K++A   GI++DP
Sbjct: 265 SGMEEY-------GL---RVNETVFEVVDDYRGPGYAIPSSEDVEILKEVASIEGIILDP 310

Query: 450 IYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQR 489
           +YT  A+     + +   K   +V+ +HTGG  G+F  ++R
Sbjct: 325 VYTAKAFRGMIEMFRNSEK---NVLFIHTGGIFGLFAQSRR 310

BLAST of Cp4.1LG09g06240 vs. ExPASy Swiss-Prot
Match: Q9V2L2 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus abyssi (strain GE5 / Orsay) OX=272844 GN=PYRAB00630 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.2e-17
Identity = 90/334 (26.95%), Postives = 142/334 (42.51%), Query Frame = 0

Query: 152 YVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGLRS 211
           YV RDDL    I GNK RKL+  L          V+T G   S HA  TA+   + GL +
Sbjct: 39  YVKRDDLTGLGIGGNKIRKLEFLLGDALSRGCDTVITIGAVHSNHAFVTALAAKKLGLGA 98

Query: 212 HLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKMLKTQAELVAGSSGSILWFDDV 271
            L+LRGE  E+L G  L+  + G  T +  +   N  +++K   E+     G        
Sbjct: 99  VLILRGE--EVLKGNYLLDKLMGIETRIYEA--DNSWELMKVAEEVAEELKG-------- 158

Query: 272 FQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVINEGAGDSIALLGLIRLVNYL-SQD 331
                                         +K  +I  G    +  LG IR V  L +Q 
Sbjct: 159 ----------------------------EGKKPYIIPPGGASPVGTLGYIRGVGELYTQV 218

Query: 332 HLLGKQRAIKFVIDA-GTGTTAIGLGLGALCLGLPWEVTAVMLADRIDGYKRQEKRLISE 391
             LG +  I  V+DA G+G T  GL LG+  +   W V  + ++   +  K + K L+ +
Sbjct: 219 KKLGLR--IDTVVDAVGSGGTYAGLLLGSAIVNAEWSVVGIDVSSATEKAKERVKNLVEK 278

Query: 392 FKKHFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVLDGEVEICKQIAQETGILVDPIY 451
            K+  G  + V + E  +          +    +G ++    ++ K +    G+L+DP+Y
Sbjct: 279 TKELLG--INVKVQEPRIYD--------YGFGAYGKIVKEVAKLIKSVGTMEGLLLDPVY 319

Query: 452 TLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMF 484
           T  A+     L++KG  +   V+ +HTGG  G+F
Sbjct: 339 TGKAFYGLMDLAKKG-DLGESVLFIHTGGLPGIF 319

BLAST of Cp4.1LG09g06240 vs. ExPASy Swiss-Prot
Match: O57809 (Putative 1-aminocyclopropane-1-carboxylate deaminase OS=Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) OX=70601 GN=PH0054 PE=1 SV=2)

HSP 1 Score: 78.2 bits (191), Expect = 3.0e-13
Identity = 85/336 (25.30%), Postives = 132/336 (39.29%), Query Frame = 0

Query: 152 YVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGLRS 211
           Y+ RDDL    I GNK RKL+  L          V+T G   S HA  T +   + GL +
Sbjct: 39  YIKRDDLTGLGIGGNKIRKLEYLLGDALSKGADVVITVGAVHSNHAFVTGLAAKKLGLDA 98

Query: 212 HLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM-LKTQAELVAGSSGSILWFDD 271
            L+LRG+  E L G  L+  I G    +   +Y  ++   L   AE +A           
Sbjct: 99  ILVLRGK--EELKGNYLLDKIMG----IETRVYDAKDSFELMKYAEEIAE---------- 158

Query: 272 VFQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVINEGAGDSIALLGLIRLVNYLSQD 331
                                      ++  RK  VI  G    I  LG +R V  ++  
Sbjct: 159 -------------------------ELKREGRKPYVIPPGGASPIGTLGYVRAVGEIA-- 218

Query: 332 HLLGKQRAIKF---VIDAGTGTTAIGLGLGALCLGLPWEVTAVMLADRIDGYKRQEKRLI 391
                Q  +KF   V+ AG+G T  GL LG   L        + +    +    +   LI
Sbjct: 219 ----TQSEVKFDSIVVAAGSGGTLAGLSLGLSILNEDIRPVGIAVGRFGEVMTSKLDNLI 278

Query: 392 SEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVLDGEVEICKQIAQETGILVDP 451
            E  +  G  V V     + + G           ++G +     +I +++    GI++DP
Sbjct: 279 KEAAELLGVKVEVRPELYDYSFG-----------EYGKITGEVAQIIRKVGTREGIILDP 315

Query: 452 IYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMF 484
           +YT  A+     L++KG ++   ++ +HTGG  G F
Sbjct: 339 VYTGKAFYGLVDLARKG-ELGEKILFIHTGGISGTF 315

BLAST of Cp4.1LG09g06240 vs. NCBI nr
Match: XP_023541195.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 999 bits (2583), Expect = 0.0
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK
Sbjct: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 499

BLAST of Cp4.1LG09g06240 vs. NCBI nr
Match: XP_023541196.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 984 bits (2543), Expect = 0.0
Identity = 494/499 (99.00%), Postives = 494/499 (99.00%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK
Sbjct: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
           GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 494

BLAST of Cp4.1LG09g06240 vs. NCBI nr
Match: XP_022944821.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 960 bits (2482), Expect = 0.0
Identity = 480/499 (96.19%), Postives = 487/499 (97.60%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS RSVGSFTSLP RPRD SSVFR RRTVVQSRMGG GFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T DTNLE
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNS+PVDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKV+VINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+G  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 497

BLAST of Cp4.1LG09g06240 vs. NCBI nr
Match: XP_022944822.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 [Cucurbita moschata])

HSP 1 Score: 945 bits (2442), Expect = 0.0
Identity = 475/499 (95.19%), Postives = 482/499 (96.59%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS RSVGSFTSLP RPRD SSVFR RRTVVQSRMGG GFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T DTNLE
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNS+PVDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKV+VINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+G  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 492

BLAST of Cp4.1LG09g06240 vs. NCBI nr
Match: XP_022966678.1 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 463/499 (92.79%), Postives = 477/499 (95.59%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS  SVGSFTS PN PR+VSSVFR RRTVVQSR+GG GFFFRATK RLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T  T+++
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNSV VDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKVI+INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVG  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 497

BLAST of Cp4.1LG09g06240 vs. ExPASy TrEMBL
Match: A0A6J1FZ47 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449244 PE=3 SV=1)

HSP 1 Score: 960 bits (2482), Expect = 0.0
Identity = 480/499 (96.19%), Postives = 487/499 (97.60%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS RSVGSFTSLP RPRD SSVFR RRTVVQSRMGG GFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T DTNLE
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNS+PVDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKV+VINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+G  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 497

BLAST of Cp4.1LG09g06240 vs. ExPASy TrEMBL
Match: A0A6J1FZ73 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449244 PE=3 SV=1)

HSP 1 Score: 945 bits (2442), Expect = 0.0
Identity = 475/499 (95.19%), Postives = 482/499 (96.59%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS RSVGSFTSLP RPRD SSVFR RRTVVQSRMGG GFFFR TKCRLLHSK
Sbjct: 1   MRQAIPKMASARSVGSFTSLPYRPRDASSVFRPRRTVVQSRMGGSGFFFRDTKCRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T DTNLE
Sbjct: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRQWALANPDSKINKVILSTEDTNLE 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNS+PVDYDFHIRARK+
Sbjct: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSLPVDYDFHIRARKY 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKV+VINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVVVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYP+G  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPIG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 492

BLAST of Cp4.1LG09g06240 vs. ExPASy TrEMBL
Match: A0A6J1HST3 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466307 PE=3 SV=1)

HSP 1 Score: 920 bits (2379), Expect = 0.0
Identity = 463/499 (92.79%), Postives = 477/499 (95.59%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS  SVGSFTS PN PR+VSSVFR RRTVVQSR+GG GFFFRATK RLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T  T+++
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNSV VDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKVI+INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVG  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 497

BLAST of Cp4.1LG09g06240 vs. ExPASy TrEMBL
Match: A0A6J1HUH2 (D-cysteine desulfhydrase 2, mitochondrial isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466307 PE=3 SV=1)

HSP 1 Score: 905 bits (2339), Expect = 0.0
Identity = 458/499 (91.78%), Postives = 472/499 (94.59%), Query Frame = 0

Query: 1   MRQAIPKMASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSK 60
           MRQAIPKMAS  SVGSFTS PN PR+VSSVFR RRTVVQSR+GG GFFFRATK RLLHSK
Sbjct: 1   MRQAIPKMASACSVGSFTSSPNWPRNVSSVFRPRRTVVQSRVGGSGFFFRATKRRLLHSK 60

Query: 61  TATSAMDNEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLE 120
           TA  AMDNEY SRKLT+SSQVVQFGGEDFVARLLDR+WALANP+SKINKVIL T  T+++
Sbjct: 61  TAKVAMDNEYYSRKLTYSSQVVQFGGEDFVARLLDRKWALANPDSKINKVILSTEGTDIK 120

Query: 121 GSPANHLCLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180
            SPANH CLAVDTNICTYNDL+R NASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED
Sbjct: 121 DSPANHSCLAVDTNICTYNDLARENASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIED 180

Query: 181 NLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240
           NLVTDVVTCGGCQSAHAAAT     ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP
Sbjct: 181 NLVTDVVTCGGCQSAHAAAT-----ERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVP 240

Query: 241 RSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKH 300
           RSIYANREKML+T+AELVAGSSGSILWFDD+FQSSIMKQPCSTTNSV VDYDFHIRARKH
Sbjct: 241 RSIYANREKMLETRAELVAGSSGSILWFDDIFQSSIMKQPCSTTNSVQVDYDFHIRARKH 300

Query: 301 ERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALC 360
           ERKVI+INEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFV+DAGTGTTAIGLGLGALC
Sbjct: 301 ERKVIIINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVVDAGTGTTAIGLGLGALC 360

Query: 361 LGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRP 420
           L LPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVG  LGEDEVNGGIVNWVERFRP
Sbjct: 361 LELPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVG--LGEDEVNGGIVNWVERFRP 420

Query: 421 RKFGNVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480
           RKFGNVL GEVE CKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL
Sbjct: 421 RKFGNVLVGEVETCKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTL 480

Query: 481 GMFGIAQRYKSYFNNLKNV 499
           GMFGIAQRYKSYFNNLKNV
Sbjct: 481 GMFGIAQRYKSYFNNLKNV 492

BLAST of Cp4.1LG09g06240 vs. ExPASy TrEMBL
Match: A0A6J1DFQ3 (D-cysteine desulfhydrase 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019685 PE=3 SV=1)

HSP 1 Score: 791 bits (2044), Expect = 7.57e-286
Identity = 393/493 (79.72%), Postives = 438/493 (88.84%), Query Frame = 0

Query: 8   MASVRSVGSFTSLPNRPRDVSSVFRSRRTVVQSRMGGFGFFFRATKCRLLHSKTATSAMD 67
           M S RSV SF S+P+RP DVS V R  RTVV+S MGG G+FF ATK RLLHSKTA +A+D
Sbjct: 1   MGSARSVCSFMSVPSRPGDVSGVLRWWRTVVESEMGGPGYFFSATKLRLLHSKTAIAAVD 60

Query: 68  NEYSSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLEGSPANHL 127
           NE+S R  T  SQ V+ GGE+FV+RLLDRRW LA+P++KI+KV    ADTNLE SPA+HL
Sbjct: 61  NEFSFRNFTDGSQGVKLGGEEFVSRLLDRRWTLADPDTKISKVTFSNADTNLENSPASHL 120

Query: 128 CLAVDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVV 187
           CLAVDTN CT+ND+SR +  EQSFYVVRDDLLHPLINGNKARKLDG LPLIEDN VTDVV
Sbjct: 121 CLAVDTNSCTFNDVSRTDYQEQSFYVVRDDLLHPLINGNKARKLDGVLPLIEDNSVTDVV 180

Query: 188 TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANR 247
           TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNL+STIYGNVTYVPR+IYANR
Sbjct: 181 TCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLISTIYGNVTYVPRAIYANR 240

Query: 248 EKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVI 307
           EKMLK+QA+LVAGSS  I+WFDD+F SS+ KQ CST NSV +DYDF+ RARKH RKVIVI
Sbjct: 241 EKMLKSQADLVAGSSDGIIWFDDIFPSSLKKQACST-NSVQIDYDFNRRARKHGRKVIVI 300

Query: 308 NEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALCLGLPWEV 367
           NEGAGD++ALLGLIRLV YLSQDHLLGKQRA+KFV+DAGTGTTA+GLGLGALCLGLPWEV
Sbjct: 301 NEGAGDAVALLGLIRLVKYLSQDHLLGKQRAVKFVVDAGTGTTAVGLGLGALCLGLPWEV 360

Query: 368 TAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVL 427
           TA+MLAD+IDGYK+QEKRLISEF+K+FGYP    LG + VNG +VNWVERF PRKFGNVL
Sbjct: 361 TALMLADKIDGYKQQEKRLISEFRKYFGYPAD--LGNNVVNGAVVNWVERFHPRKFGNVL 420

Query: 428 DGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVV-MLHTGGTLGMFGIA 487
           DGEVE C+QIAQ+TGILVDPIYTLAAWEMA+FLSQKGAK  ADVV +LHTGGTLGMFG+A
Sbjct: 421 DGEVETCRQIAQKTGILVDPIYTLAAWEMASFLSQKGAKTNADVVVLLHTGGTLGMFGLA 480

Query: 488 QRYKSYFNNLKNV 499
           QRYKSYFN+LKNV
Sbjct: 481 QRYKSYFNDLKNV 490

BLAST of Cp4.1LG09g06240 vs. TAIR 10
Match: AT3G26115.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 461.1 bits (1185), Expect = 1.2e-129
Identity = 238/428 (55.61%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 71  SSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLEGSPANHLCLA 130
           SS+++  +S++      +FV++LLDR+W L  P S I ++ +    ++++G   +     
Sbjct: 23  SSQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISV----SSVKG--IDKFSFL 82

Query: 131 VDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 190
            +T     +++S++     SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 83  NNTRPHLGDEMSKSKQG-SSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 142

Query: 191 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 250
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 143 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 202

Query: 251 LKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVINEG 310
           L+T A+LVAG  G++LW  D+ +         T N   +D DF    +   RKV+++NEG
Sbjct: 203 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 262

Query: 311 AGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALCLGLPWEVTAV 370
           AGD++ALLG+ RLV +LSQDHLLGK+R +KFV+DAGTGTTA+GLG+ A+ LGLPWE+ AV
Sbjct: 263 AGDALALLGMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAAMSLGLPWEINAV 322

Query: 371 MLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRPRKFGNVLDGE 430
           MLAD +  YKR E  LI+EF + F  P  V     ++N  ++ W++R  PRKFG VL+GE
Sbjct: 323 MLADTLKNYKRHEDHLIAEFSRQF--PGSVFCSGLDMN-QMIKWIDRQHPRKFGKVLEGE 382

Query: 431 VEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFGIAQRYK 490
           VE+C++IAQETG+LVDP+YTLAAWE AT L Q   +  + VVMLHTGGTLGMFG+AQRYK
Sbjct: 383 VEMCRKIAQETGVLVDPMYTLAAWETATELVQD--EKSSIVVMLHTGGTLGMFGLAQRYK 427

Query: 491 SYFNNLKN 499
           + F NLK+
Sbjct: 443 TCFTNLKD 427

BLAST of Cp4.1LG09g06240 vs. TAIR 10
Match: AT3G26115.2 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 453.4 bits (1165), Expect = 2.4e-127
Identity = 237/434 (54.61%), Postives = 315/434 (72.58%), Query Frame = 0

Query: 71  SSRKLTHSSQVVQFGGEDFVARLLDRRWALANPESKINKVILLTADTNLEGSPANHLCLA 130
           SS+++  +S++      +FV++LLDR+W L  P S I ++ +    ++++G   +     
Sbjct: 23  SSQRIPITSEI---DSREFVSKLLDRKWGLQCPASPIQQISV----SSVKG--IDKFSFL 82

Query: 131 VDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFLPLIEDNLVTDVVTCG 190
            +T     +++S++     SFY++RDDLLHPL+NGNKARKLD  LPL+ED+ VTD+VTCG
Sbjct: 83  NNTRPHLGDEMSKSKQG-SSFYILRDDLLHPLVNGNKARKLDALLPLVEDHKVTDLVTCG 142

Query: 191 GCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGNVTYVPRSIYANREKM 250
           GCQSAH AA AV CAERGLRSHLLLRGEQP++LTGYNL+ST+YGNV YVPRS YANRE+M
Sbjct: 143 GCQSAHTAAVAVSCAERGLRSHLLLRGEQPDVLTGYNLVSTMYGNVQYVPRSRYANREEM 202

Query: 251 LKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHIRARKHERKVIVINEG 310
           L+T A+LVAG  G++LW  D+ +         T N   +D DF    +   RKV+++NEG
Sbjct: 203 LRTYADLVAGEDGTVLWAKDIVEGR------DTMNVAKMD-DFS-SMKTSRRKVLIVNEG 262

Query: 311 AGDSIALLGLIRLVNYLSQDHLLGKQRAIKFVIDAGTGTTAIGLGLGALCLGLPWEVTAV 370
           AGD++ALLG+ RLV +LSQDHLLGK+R +KFV+DAGTGTTA+GLG+ A+ LGLPWE+ AV
Sbjct: 263 AGDALALLGMFRLVQHLSQDHLLGKKRPVKFVVDAGTGTTAVGLGVAAMSLGLPWEINAV 322

Query: 371 MLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIVNWVERFRPR------KFG 430
           MLAD +  YKR E  LI+EF + F  P  V     ++N  ++ W++R  PR      +FG
Sbjct: 323 MLADTLKNYKRHEDHLIAEFSRQF--PGSVFCSGLDMN-QMIKWIDRQHPRNSFCSCRFG 382

Query: 431 NVLDGEVEICKQIAQETGILVDPIYTLAAWEMATFLSQKGAKVKADVVMLHTGGTLGMFG 490
            VL+GEVE+C++IAQETG+LVDP+YTLAAWE AT L Q   +  + VVMLHTGGTLGMFG
Sbjct: 383 KVLEGEVEMCRKIAQETGVLVDPMYTLAAWETATELVQD--EKSSIVVMLHTGGTLGMFG 433

Query: 491 IAQRYKSYFNNLKN 499
           +AQRYK+ F NLK+
Sbjct: 443 LAQRYKTCFTNLKD 433

BLAST of Cp4.1LG09g06240 vs. TAIR 10
Match: AT1G48420.1 (D-cysteine desulfhydrase )

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-16
Identity = 95/389 (24.42%), Postives = 164/389 (42.16%), Query Frame = 0

Query: 117 TNLEGSPANHLCLA-VDTNICTYNDLSRANASEQSFYVVRDDLLHPLINGNKARKLDGFL 176
           ++L   P++   LA + T I  +N     N +E   ++ RDD     ++GNK RKL+  +
Sbjct: 44  SHLRPLPSHTFSLAHLPTPIHRWNLPGLPNGTE--LWIKRDDFTGMELSGNKVRKLEFLM 103

Query: 177 PLIEDNLVTDVVTCGGCQSAHAAATAVLCAERGLRSHLLLRGEQPELLTGYNLMSTIYGN 236
               D     V+T GG QS H  ATA       L SHL+LR    +LL   +    + GN
Sbjct: 104 AEAVDQHADTVITIGGIQSNHCRATATASNYLNLNSHLILR--TSKLLADED--PGLVGN 163

Query: 237 VTYVPRSIYANREKMLKTQAELVAGSSGSILWFDDVFQSSIMKQPCSTTNSVPVDYDFHI 296
           +           E+++     L++                  K+  S+  S  +      
Sbjct: 164 LLV---------ERLVGANVHLIS------------------KEEYSSIGSEALTNALKE 223

Query: 297 RARKHERKVIVINEGAGDSIALLGLIRLVNYLSQDHLLGKQRAIKF---VIDAGTGTTAI 356
           +  K  +K  VI  G  +S+   G I     + ++ L  +   +KF   V+  G+G T  
Sbjct: 224 KLEKEGKKPYVIPVGGSNSLGTWGYIEAAREI-EEQLNYRPDDLKFDDIVVACGSGGTIA 283

Query: 357 GLGLGALCLGLPWEVTAVMLADRIDGYKRQEKRLISEFKKHFGYPVGVGLGEDEVNGGIV 416
           G+ LG+    L  +V A  + D  D +    + L+       G   GV    D VN    
Sbjct: 284 GISLGSWLGALKAKVHAFSVCDDPDYFYDFVQGLLD------GLHAGVN-SRDIVN---- 343

Query: 417 NWVERFRPRKFGNVLDGEVEICKQIAQETGILVDPIYT-LAAWEMATFLSQ-----KGAK 476
             +   + + +      E+E  K++A  TG+++DP+Y+  AA+ +   +++     +G K
Sbjct: 344 --IHNAKGKGYAMNTSEELEFVKKVASSTGVILDPVYSGKAAYGLINEITKDPKCWEGRK 381

Query: 477 VKADVVMLHTGGTLGMFGIAQRYKSYFNN 496
               ++ +HTGG LG++    +  S   N
Sbjct: 404 ----ILFIHTGGLLGLYDKVDQMASLMGN 381