Cp4.1LG09g04930 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g04930
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAlpha-galactosidase
LocationCp4.1LG09: 3195596 .. 3199626 (-)
RNA-Seq ExpressionCp4.1LG09g04930
SyntenyCp4.1LG09g04930
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGCACTACAGAAGTCACTATAAATACTGACACTCCCACTAAGTGGCTTATCCACGATCATTAGAACCAGAGCTTCAATTTCTACGCTGCGCATACTGACGAAGACGATATTCTCGGTGAGTGAGAGATCTTTCAAGTGTAGAAATGGAGCTCCGGAGTTACTGCGGAGGAGCGGCAATGGCGGTGTTTGTTTTAGTGTTTTCTTCTGTAATGGTGGTGAGTGTTTTGGCTACTTCCAGATTGGCTGAGATCTCCGCTGCTGGTGAGTTGCTGAGGCGGAATCTGCTTGCAAATGGCCTTGGAGTTACTCCTCCGATGGGGTGAGTGCTTTGATCTTCCGCAATCTGATCGGTTTTTTGTTTTTGTTTGTTGATGTATATTCTCTGTTTTTTATTCAGATGACGCTGGTTACTTGACTGAATTTGTATGTATATATTTTTTTTTAATGTTAATTTGTTGTTCTTTTGGAACCGATTAGATTTTCGACTTTTCGTCTCTCTGTTTCAAACTTTTGATTATTATCCCGTGGAAATTTTAGGTAAACAGTGTTCTAGGAGATTTTCTTAATTTTGCTATTTTGTTATATTCGGAAGACTAGGAGAAAGGTTTGACGTATTTGGCACATACTTCTCTAATTATCTTATTCTTTTCTGTATTTGTCCTTTTCGTTCTGTTAGCGAGATGAATTTGGTCTCTCTCTCTCTGTCTTTCTTTTTGGATTAATTAAGATCATTTGTAATATCCGATTGGAGGAAATGGAATAAAGAACATTGTTGATGAAACTGTTCTTATTTTAATTTGAAAATTCCTTGTTGTTGGACAGATGGAATAGCTGGAATCATTTTGCATGCAATATCAATGAAAACATGATTAAAGATACCGGTAAATTTCATCATCCAACATCTCTCTTACTTCAGCTCTCAAAACACTAAATAATGATTAATAACAAAAAGCCCTGGTTCAACAGCTGATGCTTTGGTCTCCACTGGGCTTTCGAAGCTTGGATATGAATATGTCAATATCGGTATGGGCCGATCTCTTCTCTCTTTCTCTTATAGTTCTAGTTCAAGATTCTAAAGGTAAACTAAATGATATTTGGCTTTTCTTAGATGATTGCTGGGCTGAGATAGCTCGTGACGATAAGGTAATTTGAGATTGCATGAGAACTGTTTGATTTTATGTCTCTGAGAGTTCTGACTTCTCTTTAACATGATTTCAGGGTAATCTGGTGCCGAAGAAATCGACGTTTCCGTCTGGCATGAAGGCTCTTGCAGATTATGTTCACGCCAAGGGTCTTAAGCTTGGAATCTATTCAGATGCTGGGTAACGATAATTTTCAACTGCATAATCTATATTTTATTTTTCCATTTCAAGTCTTAATTTATCTTCTATGTTTATGTACATATTTTTTCAACTTCACTGTTTATTAGGGGCAAGACTATGCAAAGTAGATAGGTTTTACATATTTAGCTTATTGAATTAATATATGGGATAATTCCAACTCTACGGTATCTTAGAGACATTAATGCACATGAAAGATAATTGAAATTTTTTAAATGTTATAGGGAAAAAGAGGACAATTTTAGTATAGATTTTCATTTTTAGCGTTTTCAAATTTCTTTGCATATTCTCTTTATGTAAAGTCGATCTTCCATATTCACGCATTAAATTATCATGCATATGTGCAGGTATTTTACTTGCAGTAAAACCATGCCCGGCTCTCTTGGTCATGAGGAACAAGACGCTAAAACCTTTGCTTCATGGGTATGAACAACAGGGAATACTCCCTACCAAGTTTTTCAAATCATAGCATACTATGTTCACTTCTTGAAGTTCGTTGATGAACATTGCTTTATTTGGCAGGGGATTGACTATTTGAAGTATGATAATTGCAACAATGGTAACATTAAGCCAACCGTTAGGTAATGCAATTGTTACAATCATAAATCAATCAGTTCACTTCTTTTTTCAGTTTGGCCATTTGTGGGAGTTTGAAGGGTGAATCAGCCTGTTGAATATCTTTTCTTTTTCAGATATCCGGTCATGACTCGAGCGCTGATGAAGGCCGGGCGACCCATCTTCTTCTCTCTTTGTGAATGGTATAGCTAATCCTACTTATTCCCAACTCAGTATGTTCATATGAAACTATTTCTTTTCTATTATCATTTATGACAATTCCTCTCTTGCTTTGATCTGCAGGGGAGACCTGCACCCTGCTTTGTGGGGTGATAAGTTAGGAAACAGCTGGAGAACTACCAACGACATTAACGATTCGTGGGAAAGGTATCGATTCAAAGAAGATTTTGCTTAGTTTCTTATACTTTAGCATCTGAAACTCAAGGATTAAAGCATTGGAGATTGCATTTGCAGTATGATTTCTAGAGCAGACCTTAATGAACCATATGCCGATTTCGCACGGCCTGGTGGCTGGAACGGTAGTGTATTCGACATATCAGACCAAATCGTTTCTAGTAGAATATCTTTGGAATATTTTGATTAATCATTCTTTCTAACTGTTATCAGATCCTGATATGCTTGAGGTGGGAAATGGAGGGATGACTAAAGATGAGTACATTGTCCACTTTAGTCTTTGGGCCATTTCCAAGGTATTGGGCAGCCAGCAAAAACTTTAATGTTCTTGAAGATTGAAAAAAACCTGGTTGTTAATATAGTCCTTGTGGGGTGGTGGTGTTGCAGGCTCCCCTTCTTCTTGGTTGTGATTTGAGGAATCTAACAAAAGAAACAAAGGAGATCATTACAAACACGGAAGTCATTGCAGTTAACCAAGGTATAAAATATATCTCGTATGATTGAGGATATTTAGTCTTCCATCACGGTTTCTCGTAGGTATCATATTTGACTGTTTACTATAGGTAAATATCGATTATCGTGTATCCGCTTATTGTTCTCTCCATTTTCTTTGTAATTTATTTGCTTCTTTAAATGATTATAAATAGAGAACTTTCACCACCAATTAAATGATTATAAATAGAGAACTTTCACCACCAAATGGTGGTTTTATAAAATATTCTCATGGTACTAGAACCCCTTCGGTTTAGCAACCCCGATCCTTTTTTCTCCATGGCTTCTGAATCCTCTTCTCATCTTCTTCCTTTCAACACTCTAATCCATATGATCACCATCAAACTTTCCTCGTCCATTTAGGTGCTTTCCTCGTCCATTTAGGTGCTGTGGTTTTAGCAAATATTTTCAAAATACATATAAATGATCATTACACTTTCTCGGGTCATTCATGGTATTGCTCGCTTCTCAATCTCATCAACTTCGCTCGATAATTCCTGAAACAGATCCCCTCGGTATCCAGGCCAAGAAGGTCAGAAGCGAAGGCGACCTAGAGGTAAAATTCCAACACACACTCTTACTATTACCATATCATCATCCATTTTGTTGCACAACAAAGAAGACCTAAACATCCATTTGTTAACTGTCATTCCAGGTCTGGGCAGGACCTCTCTCCGACTACCGAGTAGCACTCGTGTTGCTGAACCGTGGCCCATGGCGCAGTTCGATCACCGCCCAATGGGACGACATCGGAATCCCTCCAAACAGCAACGTTCGAGCAAGAGATCTTTGGGAGGTAAGTTTCTAGCACCCACCCCCAAACAACATCATTCATGTATCCACAAAAATCTCTGATTGTGTATGAAATTTTGCAGCACACTACTTTGAAGACCATCTTTGTAGCGAATTTGACAGCCACTGTGGATTCTCATGCCTGCAAACTCTACATTTTGAACCCAATTTCTTGAGGTTAAAGACATTGTTGGCTCGGCAGAAATAAGGTTTTAAGCTGATTTATGGAACATTCTTATGAACATCTTTGTTCAATGCTTTGTTTTTAAAGCTGGGCTGTTAGTTTGAGGAAAAAGAAATAAAAGGTTCCCAACATTTCATATATTTTTATGGTTAGTACTATCTTGAAAAAAGATTCATTAATTTTCTAAATTTATATTAAACGTTTGTAGATGAGCTATGAGGTGTCGTTACTACGAGGTGTCATTAAATTTATAT

mRNA sequence

TGCACTACAGAAGTCACTATAAATACTGACACTCCCACTAAGTGGCTTATCCACGATCATTAGAACCAGAGCTTCAATTTCTACGCTGCGCATACTGACGAAGACGATATTCTCGGTGAGTGAGAGATCTTTCAAGTGTAGAAATGGAGCTCCGGAGTTACTGCGGAGGAGCGGCAATGGCGGTGTTTGTTTTAGTGTTTTCTTCTGTAATGGTGGTGAGTGTTTTGGCTACTTCCAGATTGGCTGAGATCTCCGCTGCTGGTGAGTTGCTGAGGCGGAATCTGCTTGCAAATGGCCTTGGAGTTACTCCTCCGATGGGATGGAATAGCTGGAATCATTTTGCATGCAATATCAATGAAAACATGATTAAAGATACCGCTGATGCTTTGGTCTCCACTGGGCTTTCGAAGCTTGGATATGAATATGTCAATATCGATGATTGCTGGGCTGAGATAGCTCGTGACGATAAGGGTAATCTGGTGCCGAAGAAATCGACGTTTCCGTCTGGCATGAAGGCTCTTGCAGATTATGTTCACGCCAAGGGTCTTAAGCTTGGAATCTATTCAGATGCTGGGTATTTTACTTGCAGTAAAACCATGCCCGGCTCTCTTGGTCATGAGGAACAAGACGCTAAAACCTTTGCTTCATGGGGGATTGACTATTTGAAGTATGATAATTGCAACAATGGTAACATTAAGCCAACCGTTAGATATCCGGTCATGACTCGAGCGCTGATGAAGGCCGGGCGACCCATCTTCTTCTCTCTTTGTGAATGGGGAGACCTGCACCCTGCTTTGTGGGGTGATAAGTTAGGAAACAGCTGGAGAACTACCAACGACATTAACGATTCGTGGGAAAGTATGATTTCTAGAGCAGACCTTAATGAACCATATGCCGATTTCGCACGGCCTGGTGGCTGGAACGATCCTGATATGCTTGAGGTGGGAAATGGAGGGATGACTAAAGATGAGTACATTGTCCACTTTAGTCTTTGGGCCATTTCCAAGGCCAAGAAGGTCAGAAGCGAAGGCGACCTAGAGGTCTGGGCAGGACCTCTCTCCGACTACCGAGTAGCACTCGTGTTGCTGAACCGTGGCCCATGGCGCAGTTCGATCACCGCCCAATGGGACGACATCGGAATCCCTCCAAACAGCAACGTTCGAGCAAGAGATCTTTGGGAGCACACTACTTTGAAGACCATCTTTGTAGCGAATTTGACAGCCACTGTGGATTCTCATGCCTGCAAACTCTACATTTTGAACCCAATTTCTTGAGGTTAAAGACATTGTTGGCTCGGCAGAAATAAGGTTTTAAGCTGATTTATGGAACATTCTTATGAACATCTTTGTTCAATGCTTTGTTTTTAAAGCTGGGCTGTTAGTTTGAGGAAAAAGAAATAAAAGGTTCCCAACATTTCATATATTTTTATGGTTAGTACTATCTTGAAAAAAGATTCATTAATTTTCTAAATTTATATTAAACGTTTGTAGATGAGCTATGAGGTGTCGTTACTACGAGGTGTCATTAAATTTATAT

Coding sequence (CDS)

ATGGAGCTCCGGAGTTACTGCGGAGGAGCGGCAATGGCGGTGTTTGTTTTAGTGTTTTCTTCTGTAATGGTGGTGAGTGTTTTGGCTACTTCCAGATTGGCTGAGATCTCCGCTGCTGGTGAGTTGCTGAGGCGGAATCTGCTTGCAAATGGCCTTGGAGTTACTCCTCCGATGGGATGGAATAGCTGGAATCATTTTGCATGCAATATCAATGAAAACATGATTAAAGATACCGCTGATGCTTTGGTCTCCACTGGGCTTTCGAAGCTTGGATATGAATATGTCAATATCGATGATTGCTGGGCTGAGATAGCTCGTGACGATAAGGGTAATCTGGTGCCGAAGAAATCGACGTTTCCGTCTGGCATGAAGGCTCTTGCAGATTATGTTCACGCCAAGGGTCTTAAGCTTGGAATCTATTCAGATGCTGGGTATTTTACTTGCAGTAAAACCATGCCCGGCTCTCTTGGTCATGAGGAACAAGACGCTAAAACCTTTGCTTCATGGGGGATTGACTATTTGAAGTATGATAATTGCAACAATGGTAACATTAAGCCAACCGTTAGATATCCGGTCATGACTCGAGCGCTGATGAAGGCCGGGCGACCCATCTTCTTCTCTCTTTGTGAATGGGGAGACCTGCACCCTGCTTTGTGGGGTGATAAGTTAGGAAACAGCTGGAGAACTACCAACGACATTAACGATTCGTGGGAAAGTATGATTTCTAGAGCAGACCTTAATGAACCATATGCCGATTTCGCACGGCCTGGTGGCTGGAACGATCCTGATATGCTTGAGGTGGGAAATGGAGGGATGACTAAAGATGAGTACATTGTCCACTTTAGTCTTTGGGCCATTTCCAAGGCCAAGAAGGTCAGAAGCGAAGGCGACCTAGAGGTCTGGGCAGGACCTCTCTCCGACTACCGAGTAGCACTCGTGTTGCTGAACCGTGGCCCATGGCGCAGTTCGATCACCGCCCAATGGGACGACATCGGAATCCCTCCAAACAGCAACGTTCGAGCAAGAGATCTTTGGGAGCACACTACTTTGAAGACCATCTTTGTAGCGAATTTGACAGCCACTGTGGATTCTCATGCCTGCAAACTCTACATTTTGAACCCAATTTCTTGA

Protein sequence

MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS
Homology
BLAST of Cp4.1LG09g04930 vs. ExPASy Swiss-Prot
Match: Q9FT97 (Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 6.4e-170
Identity = 284/401 (70.82%), Postives = 320/401 (79.80%), Query Frame = 0

Query: 12  MAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGWNSWNHFACNIN 71
           M + +++ SS+++  V ++  +       E+LRR+LL NGLGVTPPMGWNSWNHF+CNI+
Sbjct: 10  MPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNID 69

Query: 72  ENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFPSGMKALADYVH 131
           E MIK+TADALV+TGLSKLGY YVNIDDCWAEI+RD KG+LVPKKSTFPSG+KA+ADYVH
Sbjct: 70  EKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVH 129

Query: 132 AKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYP 191
           +KGLKLGIYSDAGYFTCSKTMPGSLG+EE DAKTFA WGIDYLKYDNCN+   KPTVRYP
Sbjct: 130 SKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYP 189

Query: 192 VMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYA 251
           VMTRALMK+GRPIF SLCEWGD+HPALWG  +GNSWRTTNDI D+W SMIS AD+NE YA
Sbjct: 190 VMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYA 249

Query: 252 DFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISK----------------------- 311
           + ARPGGWNDPDMLEVGNGGMTKDEYIVHFS+WAISK                       
Sbjct: 250 EHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANK 309

Query: 312 -------------AKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPP 371
                        AKKVR EGDLEVWAGPLS YRVAL+LLNRGP R+SITA W+DI IP 
Sbjct: 310 EVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPA 369

Query: 372 NSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 377
           NS V ARDLWEH TLK  FV NLTATVDSHACKLY+L P++
Sbjct: 370 NSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKPVA 410

BLAST of Cp4.1LG09g04930 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 1.4e-140
Identity = 227/367 (61.85%), Postives = 276/367 (75.20%), Query Frame = 0

Query: 44  RRNLLANGLGVTPPMGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAE 103
           RR+LLANGLG+TPPMGWNSWNHF CN++E +I++TADA+VS GL+ LGY+Y+N+DDCWAE
Sbjct: 12  RRSLLANGLGLTPPMGWNSWNHFRCNLDEKLIRETADAMVSKGLAALGYKYINLDDCWAE 71

Query: 104 IARDDKGNLVPKKSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDA 163
           + RD +GNLVPK STFPSG+KALADYVH+KGLKLGIYSDAG  TCSKTMPGSLGHEEQDA
Sbjct: 72  LNRDSQGNLVPKGSTFPSGIKALADYVHSKGLKLGIYSDAGTQTCSKTMPGSLGHEEQDA 131

Query: 164 KTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKL 223
           KTFASWG+DYLKYDNCNN NI P  RYP+M++AL+ +GR IFFSLCEWG+  PA W  ++
Sbjct: 132 KTFASWGVDYLKYDNCNNNNISPKERYPIMSKALLNSGRSIFFSLCEWGEEDPATWAKEV 191

Query: 224 GNSWRTTNDINDSWESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSL 283
           GNSWRTT DI+DSW SM SRAD+N+ +A +A PGGWNDPDMLEVGNGGMT  EY  HFS+
Sbjct: 192 GNSWRTTGDIDDSWSSMTSRADMNDKWASYAGPGGWNDPDMLEVGNGGMTTTEYRSHFSI 251

Query: 284 WAISKA------------------------------------KKVRSEGDLEVWAGPLSD 343
           WA++KA                                     KV++ GDLEVWAGPLS 
Sbjct: 252 WALAKAPLLIGCDIRSMDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGDLEVWAGPLSG 311

Query: 344 YRVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHAC 375
            RVA+ L NRG   ++ITA W D+G+P  + V ARDLW H+T K++    ++A VD+H  
Sbjct: 312 KRVAVALWNRGSSTATITAYWSDVGLPSTAVVNARDLWAHSTEKSV-KGQISAAVDAHDS 371

BLAST of Cp4.1LG09g04930 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 4.4e-134
Identity = 225/408 (55.15%), Postives = 281/408 (68.87%), Query Frame = 0

Query: 5   SYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGWNSWN 64
           S  GG  + V +++  S     +L         A    +RR L  NGLG TPPMGWNSWN
Sbjct: 6   SIIGGMIIVVLLMIIGS-EGGRLLEKKNRTSAEAEHYNVRRYLAENGLGQTPPMGWNSWN 65

Query: 65  HFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFPSGMK 124
           HF C+INEN++++TADA+VSTGL+ LGY+Y+N+DDCWAE+ RD +GN+VP  + FPSG+K
Sbjct: 66  HFGCDINENVVRETADAMVSTGLAALGYQYINLDDCWAELNRDSEGNMVPNAAAFPSGIK 125

Query: 125 ALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNI 184
           ALADYVH+KGLKLG+YSDAG  TCSK MPGSLGHEEQDAKTFASWG+DYLKYDNC N  I
Sbjct: 126 ALADYVHSKGLKLGVYSDAGNQTCSKRMPGSLGHEEQDAKTFASWGVDYLKYDNCENLGI 185

Query: 185 KPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRA 244
               RYP M +AL+ +GRPIFFS+CEWG   P +W   +GNSWRTT DI D+W SM S A
Sbjct: 186 SVKERYPPMGKALLSSGRPIFFSMCEWGWEDPQIWAKSIGNSWRTTGDIEDNWNSMTSIA 245

Query: 245 DLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA--------------- 304
           D N+ +A +A PGGWNDPDMLEVGNGGMT +EY  HFS+WA++KA               
Sbjct: 246 DSNDKWASYAGPGGWNDPDMLEVGNGGMTTEEYRSHFSIWALAKAPLLVGCDIRAMDDTT 305

Query: 305 ---------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSITAQW 364
                                KKV+S  DLEVWAGPLSD +VA++L NR   R+++TA W
Sbjct: 306 HELISNAEVIAVNQDKLGVQGKKVKSTNDLEVWAGPLSDNKVAVILWNRSSSRATVTASW 365

Query: 365 DDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 377
            DIG+   + V ARDLWEH+T +++    ++A +DSHACK+Y+L P S
Sbjct: 366 SDIGLQQGTTVDARDLWEHST-QSLVSGEISAEIDSHACKMYVLTPRS 411

BLAST of Cp4.1LG09g04930 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 1.2e-131
Identity = 225/393 (57.25%), Postives = 275/393 (69.97%), Query Frame = 0

Query: 16  VLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGWNSWNHFACNINENMI 75
           VL+  S+  ++   T  L +I  A     R L+ NGL ++P MGWNSWNHF CNINE +I
Sbjct: 2   VLLSFSLRFIAFTLTITLTQI--ADGFQSRMLMNNGLALSPQMGWNSWNHFQCNINETLI 61

Query: 76  KDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFPSGMKALADYVHAKGL 135
           K TADA+VS+GLS +GY+Y+NIDDCW E+ RD +G+LV K STFPSG+KAL+DYVH+KGL
Sbjct: 62  KQTADAMVSSGLSAIGYKYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGL 121

Query: 136 KLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTR 195
           KLGIYSDAG  TCS+TMPGSLGHEEQDAKTFASWGIDYLKYDNC N    P  RYP M++
Sbjct: 122 KLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSK 181

Query: 196 ALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYADFAR 255
           AL+ +GR IFFSLCEWG   PA W   +GNSWRTT DI D+W+SM   AD N+ +A +AR
Sbjct: 182 ALLNSGRSIFFSLCEWGQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYAR 241

Query: 256 PGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA-------------------------- 315
           PG WNDPDMLEVGNGGMTK+EY+ HFS+WA++KA                          
Sbjct: 242 PGSWNDPDMLEVGNGGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIA 301

Query: 316 ----------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPPNSNV 373
                     KKV+ EGDLEVWAGPLS  RVA++L NRG   ++ITA+W +IG+  +  V
Sbjct: 302 VNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIV 361

BLAST of Cp4.1LG09g04930 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 4.1e-124
Identity = 209/367 (56.95%), Postives = 254/367 (69.21%), Query Frame = 0

Query: 44  RRNLLANGLGVTPPMGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAE 103
           RR    NGLG TP MGWNSWNHF C INE +I++TADALV+TGL+KLGY+YVNIDDCWAE
Sbjct: 52  RRRAFENGLGRTPQMGWNSWNHFYCGINEQIIRETADALVNTGLAKLGYQYVNIDDCWAE 111

Query: 104 IARDDKGNLVPKKSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDA 163
            +RD +GN VP + TFPSG+KALADYVHAKGLKLGIYSDAG  TCS  MPGSL HEEQD 
Sbjct: 112 YSRDSQGNFVPNRQTFPSGIKALADYVHAKGLKLGIYSDAGSQTCSNKMPGSLDHEEQDV 171

Query: 164 KTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKL 223
           KTFASWG+DYLKYDNCN+       RY  M+ A+   G+ IFFSLCEWG  +PA W  ++
Sbjct: 172 KTFASWGVDYLKYDNCNDAGRSVMERYTRMSNAMKTYGKNIFFSLCEWGKENPATWAGRM 231

Query: 224 GNSWRTTNDINDSWESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSL 283
           GNSWRTT DI D+W SM SRAD N+ +A +A PGGWNDPDMLEVGNGGM++ EY  HFS+
Sbjct: 232 GNSWRTTGDIADNWGSMTSRADENDQWAAYAGPGGWNDPDMLEVGNGGMSEAEYRSHFSI 291

Query: 284 WAISKA------------------------------------KKVRSEGDLEVWAGPLSD 343
           WA++KA                                    KKV+S+  LEVWAGPLS+
Sbjct: 292 WALAKAPLLIGCDVRSMSQQTKNILSNSEVIAVNQDSLGVQGKKVQSDNGLEVWAGPLSN 351

Query: 344 YRVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHAC 375
            R A+VL NR  ++++ITA W +IG+  +  V ARDLW H++        ++A+V  H C
Sbjct: 352 NRKAVVLWNRQSYQATITAHWSNIGLAGSVAVTARDLWAHSSFAA--QGQISASVAPHDC 411

BLAST of Cp4.1LG09g04930 vs. NCBI nr
Match: XP_023541761.1 (alpha-galactosidase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 764 bits (1972), Expect = 5.15e-278
Identity = 376/412 (91.26%), Postives = 376/412 (91.26%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGIQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS
Sbjct: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 412

BLAST of Cp4.1LG09g04930 vs. NCBI nr
Match: KAG6573765.1 (Alpha-galactosidase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 759 bits (1959), Expect = 4.94e-276
Identity = 373/412 (90.53%), Postives = 375/412 (91.02%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAMAVFVLVFSSVMV+SVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVMSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNV+ARDLWEHTTLKTIFVANLTATVDSHACKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

BLAST of Cp4.1LG09g04930 vs. NCBI nr
Match: KAG7012838.1 (Alpha-galactosidase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 758 bits (1958), Expect = 7.01e-276
Identity = 373/412 (90.53%), Postives = 374/412 (90.78%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAMAVFV VFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNV+ARDLWEHTTLKTIFVANLTATVDSHACKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

BLAST of Cp4.1LG09g04930 vs. NCBI nr
Match: XP_022945946.1 (alpha-galactosidase 1-like [Cucurbita moschata])

HSP 1 Score: 758 bits (1958), Expect = 7.01e-276
Identity = 373/412 (90.53%), Postives = 373/412 (90.53%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAM VFV VFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMGVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

BLAST of Cp4.1LG09g04930 vs. NCBI nr
Match: XP_022966950.1 (alpha-galactosidase 1-like [Cucurbita maxima])

HSP 1 Score: 758 bits (1957), Expect = 9.96e-276
Identity = 373/412 (90.53%), Postives = 375/412 (91.02%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGIQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNV+ARDLWEHTTLKTIFVANLTATV+SHACKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVNSHACKLYILKPIS 412

BLAST of Cp4.1LG09g04930 vs. ExPASy TrEMBL
Match: A0A6J1G2A9 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111450037 PE=3 SV=1)

HSP 1 Score: 758 bits (1958), Expect = 3.39e-276
Identity = 373/412 (90.53%), Postives = 373/412 (90.53%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAM VFV VFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMGVFVFVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILKPIS 412

BLAST of Cp4.1LG09g04930 vs. ExPASy TrEMBL
Match: A0A6J1HT03 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466509 PE=3 SV=1)

HSP 1 Score: 758 bits (1957), Expect = 4.82e-276
Identity = 373/412 (90.53%), Postives = 375/412 (91.02%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60
           MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW
Sbjct: 1   MELRSYCGGAAMAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGW 60

Query: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120
           NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP
Sbjct: 61  NSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFP 120

Query: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180
           SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN
Sbjct: 121 SGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCN 180

Query: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240
           NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM
Sbjct: 181 NGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESM 240

Query: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA----------- 300
           ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA           
Sbjct: 241 ISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRNL 300

Query: 301 -------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360
                                    KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI
Sbjct: 301 TKETKEIITNTEVIAVNQDPLGIQAKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSI 360

Query: 361 TAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           TAQWDDIGIPPNSNV+ARDLWEHTTLKTIFVANLTATV+SHACKLYIL PIS
Sbjct: 361 TAQWDDIGIPPNSNVQARDLWEHTTLKTIFVANLTATVNSHACKLYILKPIS 412

BLAST of Cp4.1LG09g04930 vs. ExPASy TrEMBL
Match: A0A6J1CC22 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010280 PE=3 SV=1)

HSP 1 Score: 704 bits (1818), Expect = 8.19e-255
Identity = 345/415 (83.13%), Postives = 359/415 (86.51%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLV---FSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPP 60
           ME RSYCGGAAMA+ VLV   +SSVMV SV ATSR+ EI+A  ELLRRNLLANGLGVTPP
Sbjct: 1   MECRSYCGGAAMAMAVLVLVVYSSVMVASVSATSRMTEITADDELLRRNLLANGLGVTPP 60

Query: 61  MGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKS 120
           MGWNSWNHFACNINE MIK+TADAL+STGLSKLGY+YVNIDDCWAEIARDDKGNLVPKKS
Sbjct: 61  MGWNSWNHFACNINEKMIKETADALISTGLSKLGYKYVNIDDCWAEIARDDKGNLVPKKS 120

Query: 121 TFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYD 180
           TFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYD
Sbjct: 121 TFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYD 180

Query: 181 NCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW 240
           NCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW
Sbjct: 181 NCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSW 240

Query: 241 ESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA-------- 300
           ESMISRADLNE YAD ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWA+SKA        
Sbjct: 241 ESMISRADLNEVYADLARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWALSKAPLLLGCDL 300

Query: 301 ----------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWR 360
                                       KKVRSEGDLEVWAGPLS YRVA+VLLNRGPWR
Sbjct: 301 RNLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSSYRVAVVLLNRGPWR 360

Query: 361 SSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           S +TAQWDDIGIPPNS V+ARDLWEH TLK +FV NL+ATVDSHACK+YIL PIS
Sbjct: 361 SVLTAQWDDIGIPPNSIVQARDLWEHNTLKNVFVGNLSATVDSHACKMYILKPIS 415

BLAST of Cp4.1LG09g04930 vs. ExPASy TrEMBL
Match: Q2MK92 (Alpha-galactosidase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 698 bits (1802), Expect = 2.08e-252
Identity = 340/413 (82.32%), Postives = 357/413 (86.44%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMV-VSVLATSRLAEISAAGELLRRNLLANGLGVTPPMG 60
           ME RSYC   A+ VFVL FS VM+  +V ATSR+ EI++ G+LLRRNLLANGLGVTPPMG
Sbjct: 1   MECRSYCKAPAVVVFVLAFSLVMLETTVSATSRMTEIASDGDLLRRNLLANGLGVTPPMG 60

Query: 61  WNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTF 120
           WNSWNHFACNINE MI++TADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPK STF
Sbjct: 61  WNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNSTF 120

Query: 121 PSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNC 180
           PSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDNC
Sbjct: 121 PSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDNC 180

Query: 181 NNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWES 240
           NNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWES
Sbjct: 181 NNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWES 240

Query: 241 MISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA---------- 300
           MISRADLNE YAD+ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA          
Sbjct: 241 MISRADLNEIYADYARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLRN 300

Query: 301 --------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSS 360
                                     KKVRSEGDLEVWAGPLS YRVA+VLLNRGPWR++
Sbjct: 301 LTKETKAIVTNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRNA 360

Query: 361 ITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           I+AQWDDIGIPPNSNV ARDLWEHTTLKT FVANLTATVDSHACKLYIL PIS
Sbjct: 361 ISAQWDDIGIPPNSNVEARDLWEHTTLKTTFVANLTATVDSHACKLYILKPIS 413

BLAST of Cp4.1LG09g04930 vs. ExPASy TrEMBL
Match: A0A1S3BDT4 (Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488793 PE=3 SV=1)

HSP 1 Score: 697 bits (1800), Expect = 4.36e-252
Identity = 345/414 (83.33%), Postives = 357/414 (86.23%), Query Frame = 0

Query: 1   MELRSYCGGAAMAVFVLVFSSVMVVS-VLATSRLAEISAA-GELLRRNLLANGLGVTPPM 60
           ME RSYC GAA+AVFVL FS VM+ S V ATSR  EI A  G+LLRRNLLANGLGVTPPM
Sbjct: 1   MECRSYCTGAAVAVFVLAFSLVMLESTVSATSRRTEIIATDGDLLRRNLLANGLGVTPPM 60

Query: 61  GWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKST 120
           GWNSWNHFACNINE MI++TADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPK ST
Sbjct: 61  GWNSWNHFACNINEKMIRETADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKNST 120

Query: 121 FPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDN 180
           FPSGMKALADYVHAKGLK+GIYSDAGYFTCSKTMPGSLGHEEQDAKTFA+WGIDYLKYDN
Sbjct: 121 FPSGMKALADYVHAKGLKIGIYSDAGYFTCSKTMPGSLGHEEQDAKTFAAWGIDYLKYDN 180

Query: 181 CNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240
           CNNGNIKPT+RYPVMTRALMKAGRPIF SLCEWGDLHPALWGDKLGNSWRTTNDINDSWE
Sbjct: 181 CNNGNIKPTIRYPVMTRALMKAGRPIFLSLCEWGDLHPALWGDKLGNSWRTTNDINDSWE 240

Query: 241 SMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA--------- 300
           SMISRADLNE YAD+ARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA         
Sbjct: 241 SMISRADLNEIYADYARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKAPLLLGCDLR 300

Query: 301 ---------------------------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRS 360
                                      KKVRSEGDLEVWAGPLS YRVA+VLLNRGPWR+
Sbjct: 301 NLTKETKEIITNTEVIAVNQDPLGVQAKKVRSEGDLEVWAGPLSGYRVAVVLLNRGPWRN 360

Query: 361 SITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 376
           +ITAQWDDIGIPPNSNV ARDLWE TTLKT FVANLTATVDSHACKLYIL PIS
Sbjct: 361 AITAQWDDIGIPPNSNVEARDLWERTTLKTTFVANLTATVDSHACKLYILKPIS 414

BLAST of Cp4.1LG09g04930 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 598.2 bits (1541), Expect = 4.6e-171
Identity = 284/401 (70.82%), Postives = 320/401 (79.80%), Query Frame = 0

Query: 12  MAVFVLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGWNSWNHFACNIN 71
           M + +++ SS+++  V ++  +       E+LRR+LL NGLGVTPPMGWNSWNHF+CNI+
Sbjct: 10  MPILMILISSMVMTMVESSRSVNNGHDDSEILRRHLLTNGLGVTPPMGWNSWNHFSCNID 69

Query: 72  ENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFPSGMKALADYVH 131
           E MIK+TADALV+TGLSKLGY YVNIDDCWAEI+RD KG+LVPKKSTFPSG+KA+ADYVH
Sbjct: 70  EKMIKETADALVTTGLSKLGYNYVNIDDCWAEISRDSKGSLVPKKSTFPSGIKAVADYVH 129

Query: 132 AKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYP 191
           +KGLKLGIYSDAGYFTCSKTMPGSLG+EE DAKTFA WGIDYLKYDNCN+   KPTVRYP
Sbjct: 130 SKGLKLGIYSDAGYFTCSKTMPGSLGYEEHDAKTFAEWGIDYLKYDNCNSDGSKPTVRYP 189

Query: 192 VMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYA 251
           VMTRALMK+GRPIF SLCEWGD+HPALWG  +GNSWRTTNDI D+W SMIS AD+NE YA
Sbjct: 190 VMTRALMKSGRPIFHSLCEWGDMHPALWGSPVGNSWRTTNDIKDTWLSMISIADMNEVYA 249

Query: 252 DFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISK----------------------- 311
           + ARPGGWNDPDMLEVGNGGMTKDEYIVHFS+WAISK                       
Sbjct: 250 EHARPGGWNDPDMLEVGNGGMTKDEYIVHFSIWAISKAPLLLGCDIRNMTKETMEIVANK 309

Query: 312 -------------AKKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPP 371
                        AKKVR EGDLEVWAGPLS YRVAL+LLNRGP R+SITA W+DI IP 
Sbjct: 310 EVIAINQDPHGVQAKKVRMEGDLEVWAGPLSGYRVALLLLNRGPSRTSITALWEDIEIPA 369

Query: 372 NSNVRARDLWEHTTLKTIFVANLTATVDSHACKLYILNPIS 377
           NS V ARDLWEH TLK  FV NLTATVDSHACKLY+L P++
Sbjct: 370 NSIVEARDLWEHQTLKQKFVGNLTATVDSHACKLYVLKPVA 410

BLAST of Cp4.1LG09g04930 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 473.0 bits (1216), Expect = 2.2e-133
Identity = 218/366 (59.56%), Postives = 264/366 (72.13%), Query Frame = 0

Query: 43  LRRNLLANGLGVTPPMGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWA 102
           + R L+ NGL ++P MGWNSWNHF CNINE +IK TADA+VS+GLS +GY+Y+NIDDCW 
Sbjct: 1   MSRMLMNNGLALSPQMGWNSWNHFQCNINETLIKQTADAMVSSGLSAIGYKYINIDDCWG 60

Query: 103 EIARDDKGNLVPKKSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQD 162
           E+ RD +G+LV K STFPSG+KAL+DYVH+KGLKLGIYSDAG  TCS+TMPGSLGHEEQD
Sbjct: 61  ELKRDSQGSLVAKASTFPSGIKALSDYVHSKGLKLGIYSDAGTLTCSQTMPGSLGHEEQD 120

Query: 163 AKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDK 222
           AKTFASWGIDYLKYDNC N    P  RYP M++AL+ +GR IFFSLCEWG   PA W   
Sbjct: 121 AKTFASWGIDYLKYDNCENTGTSPRERYPKMSKALLNSGRSIFFSLCEWGQEDPATWAGD 180

Query: 223 LGNSWRTTNDINDSWESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFS 282
           +GNSWRTT DI D+W+SM   AD N+ +A +ARPG WNDPDMLEVGNGGMTK+EY+ HFS
Sbjct: 181 IGNSWRTTGDIQDNWKSMTLIADQNDRWASYARPGSWNDPDMLEVGNGGMTKEEYMSHFS 240

Query: 283 LWAISKA------------------------------------KKVRSEGDLEVWAGPLS 342
           +WA++KA                                    KKV+ EGDLEVWAGPLS
Sbjct: 241 IWALAKAPLLIGCDLRSMDKVTFELLSNKEVIAVNQDKLGIQGKKVKKEGDLEVWAGPLS 300

Query: 343 DYRVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHA 373
             RVA++L NRG   ++ITA+W +IG+  +  V ARDLWEH+T   +    L+A V+ HA
Sbjct: 301 KKRVAVILWNRGSASANITARWAEIGLNSSDIVNARDLWEHSTYSCV-KKQLSALVEPHA 360

BLAST of Cp4.1LG09g04930 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 471.1 bits (1211), Expect = 8.4e-133
Identity = 225/393 (57.25%), Postives = 275/393 (69.97%), Query Frame = 0

Query: 16  VLVFSSVMVVSVLATSRLAEISAAGELLRRNLLANGLGVTPPMGWNSWNHFACNINENMI 75
           VL+  S+  ++   T  L +I  A     R L+ NGL ++P MGWNSWNHF CNINE +I
Sbjct: 2   VLLSFSLRFIAFTLTITLTQI--ADGFQSRMLMNNGLALSPQMGWNSWNHFQCNINETLI 61

Query: 76  KDTADALVSTGLSKLGYEYVNIDDCWAEIARDDKGNLVPKKSTFPSGMKALADYVHAKGL 135
           K TADA+VS+GLS +GY+Y+NIDDCW E+ RD +G+LV K STFPSG+KAL+DYVH+KGL
Sbjct: 62  KQTADAMVSSGLSAIGYKYINIDDCWGELKRDSQGSLVAKASTFPSGIKALSDYVHSKGL 121

Query: 136 KLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFASWGIDYLKYDNCNNGNIKPTVRYPVMTR 195
           KLGIYSDAG  TCS+TMPGSLGHEEQDAKTFASWGIDYLKYDNC N    P  RYP M++
Sbjct: 122 KLGIYSDAGTLTCSQTMPGSLGHEEQDAKTFASWGIDYLKYDNCENTGTSPRERYPKMSK 181

Query: 196 ALMKAGRPIFFSLCEWGDLHPALWGDKLGNSWRTTNDINDSWESMISRADLNEPYADFAR 255
           AL+ +GR IFFSLCEWG   PA W   +GNSWRTT DI D+W+SM   AD N+ +A +AR
Sbjct: 182 ALLNSGRSIFFSLCEWGQEDPATWAGDIGNSWRTTGDIQDNWKSMTLIADQNDRWASYAR 241

Query: 256 PGGWNDPDMLEVGNGGMTKDEYIVHFSLWAISKA-------------------------- 315
           PG WNDPDMLEVGNGGMTK+EY+ HFS+WA++KA                          
Sbjct: 242 PGSWNDPDMLEVGNGGMTKEEYMSHFSIWALAKAPLLIGCDLRSMDKVTFELLSNKEVIA 301

Query: 316 ----------KKVRSEGDLEVWAGPLSDYRVALVLLNRGPWRSSITAQWDDIGIPPNSNV 373
                     KKV+ EGDLEVWAGPLS  RVA++L NRG   ++ITA+W +IG+  +  V
Sbjct: 302 VNQDKLGIQGKKVKKEGDLEVWAGPLSKKRVAVILWNRGSASANITARWAEIGLNSSDIV 361

BLAST of Cp4.1LG09g04930 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 412.1 bits (1058), Expect = 4.6e-115
Identity = 198/366 (54.10%), Postives = 241/366 (65.85%), Query Frame = 0

Query: 48  LANGLGVTPPMGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARD 107
           L NGL  TP MGWNSWN FACNINE +IK+TADALVS+GL+ LGY +VNIDDCW+ + RD
Sbjct: 65  LNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNIDDCWSNLLRD 124

Query: 108 DKGNLVPKKSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFA 167
            +G LVP   TFPSG+K LADYVH+KGLKLGIYSDAG FTC +  PGSL HE  DA  FA
Sbjct: 125 SEGQLVPHPETFPSGIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFA 184

Query: 168 SWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSW 227
           SWG+DYLKYDNC N  IKP  RYP M  AL   GR IF+SLCEWG   PALW  ++GNSW
Sbjct: 185 SWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSW 244

Query: 228 RTTNDINDSWESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAIS 287
           RTT+DIND+W SM + ADLN  +A +A PGGWNDPDMLE+GNGGMT +EY  HFS+WA+ 
Sbjct: 245 RTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALM 304

Query: 288 KA------------------------------------KKVRSEGD---LEVWAGPLSDY 347
           KA                                    +K+++ G+    +VW+GPLS  
Sbjct: 305 KAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGD 364

Query: 348 RVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACK 375
           R+ + L NR    ++ITA WD IG+    +V  RDLW+H  +      +  A VD+H C 
Sbjct: 365 RMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCH 424

BLAST of Cp4.1LG09g04930 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.4e-100
Identity = 184/366 (50.27%), Postives = 224/366 (61.20%), Query Frame = 0

Query: 48  LANGLGVTPPMGWNSWNHFACNINENMIKDTADALVSTGLSKLGYEYVNIDDCWAEIARD 107
           L NGL  TP MGWNSWN FACNINE +IK+TADALVS+GL+ LGY +VNI          
Sbjct: 65  LNNGLARTPQMGWNSWNFFACNINETVIKETADALVSSGLADLGYIHVNI---------- 124

Query: 108 DKGNLVPKKSTFPSGMKALADYVHAKGLKLGIYSDAGYFTCSKTMPGSLGHEEQDAKTFA 167
                         G+K LADYVH+KGLKLGIYSDAG FTC +  PGSL HE  DA  FA
Sbjct: 125 --------------GIKLLADYVHSKGLKLGIYSDAGVFTC-EVHPGSLFHEVDDADIFA 184

Query: 168 SWGIDYLKYDNCNNGNIKPTVRYPVMTRALMKAGRPIFFSLCEWGDLHPALWGDKLGNSW 227
           SWG+DYLKYDNC N  IKP  RYP M  AL   GR IF+SLCEWG   PALW  ++GNSW
Sbjct: 185 SWGVDYLKYDNCFNLGIKPIERYPPMRDALNATGRSIFYSLCEWGVDDPALWAKEVGNSW 244

Query: 228 RTTNDINDSWESMISRADLNEPYADFARPGGWNDPDMLEVGNGGMTKDEYIVHFSLWAIS 287
           RTT+DIND+W SM + ADLN  +A +A PGGWNDPDMLE+GNGGMT +EY  HFS+WA+ 
Sbjct: 245 RTTDDINDTWASMTTIADLNNKWAAYAGPGGWNDPDMLEIGNGGMTYEEYRGHFSIWALM 304

Query: 288 KA------------------------------------KKVRSEGD---LEVWAGPLSDY 347
           KA                                    +K+++ G+    +VW+GPLS  
Sbjct: 305 KAPLLIGCDVRNMTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGD 364

Query: 348 RVALVLLNRGPWRSSITAQWDDIGIPPNSNVRARDLWEHTTLKTIFVANLTATVDSHACK 375
           R+ + L NR    ++ITA WD IG+    +V  RDLW+H  +      +  A VD+H C 
Sbjct: 365 RMVVALWNRCSEPATITASWDMIGLESTISVSVRDLWQHKDVTENTSGSFEAQVDAHDCH 405

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FT976.4e-17070.82Alpha-galactosidase 1 OS=Arabidopsis thaliana OX=3702 GN=AGAL1 PE=2 SV=1[more]
Q426561.4e-14061.85Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1[more]
P147494.4e-13455.15Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1[more]
Q8RX861.2e-13157.25Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1[more]
Q9FXT44.1e-12456.95Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=... [more]
Match NameE-valueIdentityDescription
XP_023541761.15.15e-27891.26alpha-galactosidase 1-like [Cucurbita pepo subsp. pepo][more]
KAG6573765.14.94e-27690.53Alpha-galactosidase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7012838.17.01e-27690.53Alpha-galactosidase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022945946.17.01e-27690.53alpha-galactosidase 1-like [Cucurbita moschata][more]
XP_022966950.19.96e-27690.53alpha-galactosidase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G2A93.39e-27690.53Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111450037 PE=3 SV=1[more]
A0A6J1HT034.82e-27690.53Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111466509 PE=3 SV=1[more]
A0A6J1CC228.19e-25583.13Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111010280 PE=3 SV=1[more]
Q2MK922.08e-25282.32Alpha-galactosidase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
A0A1S3BDT44.36e-25283.33Alpha-galactosidase OS=Cucumis melo OX=3656 GN=LOC103488793 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G08380.14.6e-17170.82alpha-galactosidase 1 [more]
AT5G08370.22.2e-13359.56alpha-galactosidase 2 [more]
AT5G08370.18.4e-13357.25alpha-galactosidase 2 [more]
AT3G56310.14.6e-11554.10Melibiase family protein [more]
AT3G56310.21.4e-10050.27Melibiase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002241Glycoside hydrolase, family 27PRINTSPR00740GLHYDRLASE27coord: 254..273
score: 69.75
coord: 86..101
score: 65.94
coord: 50..69
score: 69.5
coord: 189..207
score: 55.79
coord: 275..296
score: 34.77
coord: 162..179
score: 76.39
coord: 127..148
score: 62.27
IPR002241Glycoside hydrolase, family 27PFAMPF16499Melibiase_2coord: 55..294
e-value: 6.4E-69
score: 232.3
IPR002241Glycoside hydrolase, family 27PANTHERPTHR11452ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASEcoord: 12..289
coord: 288..375
IPR002241Glycoside hydrolase, family 27CDDcd14792GH27coord: 56..289
e-value: 5.10117E-135
score: 384.6
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 291..375
e-value: 2.2E-25
score: 90.4
IPR041233Alpha galactosidase, C-terminal beta sandwich domainPFAMPF17801Melibiase_Ccoord: 295..372
e-value: 1.9E-19
score: 69.5
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 48..290
e-value: 2.6E-115
score: 386.7
NoneNo IPR availablePANTHERPTHR11452:SF82ALPHA-GALACTOSIDASEcoord: 12..289
coord: 288..375
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 284..372
IPR000111Glycoside hydrolase family 27/36, conserved sitePROSITEPS00512ALPHA_GALACTOSIDASEcoord: 91..107
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 49..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g04930.1Cp4.1LG09g04930.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009505 plant-type cell wall
molecular_function GO:0052692 raffinose alpha-galactosidase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds