Cp4.1LG09g04500 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g04500
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	aspartyl protease AED3-like
Location	Cp4.1LG09: 2910782 .. 2913386 (+)
RNA-Seq Expression	Cp4.1LG09g04500
Synteny	Cp4.1LG09g04500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCCACACTCTATTTCAAATCAAAAGCCATGAACACAAAACCATCCACTTTCCTCTTCTCTCTCCTCCTCCTCCTCCTCCTCCGCCTCCTCGCCGCCGCTTCCGGCGGCTCCCTCCGCGTCTTCCACATCTCCACCCACTCCAAGCCGCTGTCATGGGCCGACGGAGTACTCCAAATGCAGGCCAAGGACGAAGCTCGTCTCCAGCTACTCTCCTCCCTAGTCGCCCGCCGCTCCGACGCGCCCATCGCCTCCGGCCGCCAGGTGATCCAGAGCCCCTCGTACGTGGTCCGGGCAAACATCGGAACCCCTGCCCAGACGCTGCTATTGGCCCTGGATACCAGCAATGATGCCACGTGGATTCCGTGTTCCGGCTGCGTGGGCTGCCCGGGAACCTCCGTTTTCTCCTCTGATAAATCCTCCTCCTTCCGGCCGCTCCCCTGCCAGTCGCCGCAATGCAGCCAGGTAGGGCCCACTCCATTTTCTTTAATTATAAATAAATAAATAAATTCATGATATTTTAAGGGCATTATTGTCAGAAAAACAGTTCTTATTGGATTTCCGTACTAATTAAAAAAAAGTCTTTTTATTTTCATATATTATTGGGCATCTCTATAATGTCTGTCTGTCAGTCACCGACTGACTGTTCAGTCACGGACCAACAAAGTTTTCCAATTCCCTGTCTACACTTGCACGTGATCTCTTGTTTTTTTCTAATTAATAAATATATTATTCATTTTTGTACTAATAATATTCTTACCATTTAAAAATAAAAGATAAAATACACTTATTGTAATTTTTTAATAATTTTATTTAATATTATTGAATTTTTTAAACATTTTTTTATAGAAATTTTAGAAATTTTAATTATATTTTTCAAATAAATTACAAAATTTAAGAAATTTTTTATTAATTAATGTACTCTTAAAAGTTTTAATAATATTCTAAACTTATTTAAATAAAAACTATTAATATTTTATTTAAAACTATTTGACTTTTAGAAAAAAAAAATTGGAGGGTATTTTTGAAAATTTAAGTATAAGTTTTAAACCAATTACAAAATTTAAGAGGTTTTTTATTAATTTAATATTTTTTTTTACTTTTAACCAAATTATAAATTTAGGTTATGAATTTTCAAAATTAAAATTTGGAATTTTAGTATGAAATAAGATTATATTTAAATTTATAATTAATTAATTATTTAATTTTAGAATTTGAAAAAATATTTATGACGCAGAAAATTTATTTTAACGTTAATCTCAGGTACCGAACCCCAGTTGCGGTACCAGCGCGTGCGGTTTCAACATGACATACGGCAGCTCGACGGTGGCGGCGGATTTGGTTCAAGACAACATAACGCTGGCTAGGGACTCGGTTCCGGCGTTCACATTCGGGTGCATCACGAAGGCCACCGGGAGCTCAGTGCCGCCGCAGGGGCTGTTGGGGCTGGGCCGTGGCTCATTGTCGCTCTTGTCGCAGACCCAGAGTCTGTACCAGTCCACATTCTCCTACTGCCTTCCCAGCTTCAAATCGGCTAGCTTTTCTGGGTCGTTGCGGCTCGGGCCAGTGGCTCAGCCTAAGAGGATTAAGTATACGCCGCTCCTCCGCAACCCGAGACGGTCGTCGTTGTATTATGTCAATTTGATCGGGATTAGAGTCGGTAGTAAAGTCGTCGACATTCCTCCTAGTGCTTTCGCTTTTAACACCGCCACCGGCTCCGGCACCATCATTGATTCTGGTAAGATTGTTGGTCGAGAGTCCTACCTTAACTAATTTAAGGAATGATCGTAGATTTATAAATAAATAATACATTTAAAAAATCAAAAACAAAATTATTATAGCTTATGATCATATTATTAAATGTAAGTTTAATTTTGATTGGTATAATTATTTAGGGACGACGTTCACAAGACTAGTGGCGCCGGCGTACACGGCGGTGAGGGACGAGTTCCAGCGAAGAGTAGGCAACGCAACCGTCTCATCTCTTGGTGGCTTCGACACTTGCTACACCGTCCCCATCGTCTCCCCCACCATAACCTTCATGTTCGACGCAATGAACGTCACTCTCCCACCGGACAACTTCCTCATCCACAGCACCGCCGGATCCACCACCTGCCTCGCCATGGCCTCGGCACCCGACAATGTCAACTCTGTCCTCAACGTTATTGCCAACATGCAACAACAAAACCACCGTATCGTCTTCGACATTCCCAATTCCCGTGTCGGTGTCGCCCGCGAACCGTGCTCTTAGAACGTGTCGTCTCCGATTCCGGTGATGTTTTACTTTCGGAGGTCTTAGGATAAGAGTGTTCGGTCGTGGTTTGAGTTGGTTATGGGGAAGGGGTAAAATCGTAATTTTGAATTGGTGTTGAATTGAAACGAGTGTTTTTATGGTTGTGTTATTGTGTTTCTTTTTATTTATTATTAGTTTGACATTTTTATAAAAATATTTATTTTTAAATTTATATTTCAAAGACCATTAATATAATTATGATGTTTTATTTAATTATATTATAAAGTTGTTAAATTATAATAATGTCGAACTGTGGACAAAATTAAAATACCATCTACAAGAAAAGAATATTTGAGACGACACAAAAAAAAAAAA

mRNA sequence

TCCACACTCTATTTCAAATCAAAAGCCATGAACACAAAACCATCCACTTTCCTCTTCTCTCTCCTCCTCCTCCTCCTCCTCCGCCTCCTCGCCGCCGCTTCCGGCGGCTCCCTCCGCGTCTTCCACATCTCCACCCACTCCAAGCCGCTGTCATGGGCCGACGGAGTACTCCAAATGCAGGCCAAGGACGAAGCTCGTCTCCAGCTACTCTCCTCCCTAGTCGCCCGCCGCTCCGACGCGCCCATCGCCTCCGGCCGCCAGGTGATCCAGAGCCCCTCGTACGTGGTCCGGGCAAACATCGGAACCCCTGCCCAGACGCTGCTATTGGCCCTGGATACCAGCAATGATGCCACGTGGATTCCGTGTTCCGGCTGCGTGGGCTGCCCGGGAACCTCCGTTTTCTCCTCTGATAAATCCTCCTCCTTCCGGCCGCTCCCCTGCCAGTCGCCGCAATGCAGCCAGGTACCGAACCCCAGTTGCGGTACCAGCGCGTGCGGTTTCAACATGACATACGGCAGCTCGACGGTGGCGGCGGATTTGGTTCAAGACAACATAACGCTGGCTAGGGACTCGGTTCCGGCGTTCACATTCGGGTGCATCACGAAGGCCACCGGGAGCTCAGTGCCGCCGCAGGGGCTGTTGGGGCTGGGCCGTGGCTCATTGTCGCTCTTGTCGCAGACCCAGAGTCTGTACCAGTCCACATTCTCCTACTGCCTTCCCAGCTTCAAATCGGCTAGCTTTTCTGGGTCGTTGCGGCTCGGGCCAGTGGCTCAGCCTAAGAGGATTAAGTATACGCCGCTCCTCCGCAACCCGAGACGGTCGTCGTTGTATTATGTCAATTTGATCGGGATTAGAGTCGGTAGTAAAGTCGTCGACATTCCTCCTAGGACGACGTTCACAAGACTAGTGGCGCCGGCGTACACGGCGGTGAGGGACGAGTTCCAGCGAAGAGTAGGCAACGCAACCGTCTCATCTCTTGGTGGCTTCGACACTTGCTACACCGTCCCCATCGTCTCCCCCACCATAACCTTCATGTTCGACGCAATGAACGTCACTCTCCCACCGGACAACTTCCTCATCCACAGCACCGCCGGATCCACCACCTGCCTCGCCATGGCCTCGGCACCCGACAATGTCAACTCTGTCCTCAACGTTATTGCCAACATGCAACAACAAAACCACCGTATCGTCTTCGACATTCCCAATTCCCGTGTCGGTGTCGCCCGCGAACCGTGCTCTTAGAACGTGTCGTCTCCGATTCCGGTGATGTTTTACTTTCGGAGGTCTTAGGATAAGAGTGTTCGGTCGTGGTTTGAGTTGGTTATGGGGAAGGGGTAAAATCGTAATTTTGAATTGGTGTTGAATTGAAACGAGTGTTTTTATGGTTGTGTTATTGTGTTTCTTTTTATTTATTATTAGTTTGACATTTTTATAAAAATATTTATTTTTAAATTTATATTTCAAAGACCATTAATATAATTATGATGTTTTATTTAATTATATTATAAAGTTGTTAAATTATAATAATGTCGAACTGTGGACAAAATTAAAATACCATCTACAAGAAAAGAATATTTGAGACGACACAAAAAAAAAAAA

Coding sequence (CDS)

ATGAACACAAAACCATCCACTTTCCTCTTCTCTCTCCTCCTCCTCCTCCTCCTCCGCCTCCTCGCCGCCGCTTCCGGCGGCTCCCTCCGCGTCTTCCACATCTCCACCCACTCCAAGCCGCTGTCATGGGCCGACGGAGTACTCCAAATGCAGGCCAAGGACGAAGCTCGTCTCCAGCTACTCTCCTCCCTAGTCGCCCGCCGCTCCGACGCGCCCATCGCCTCCGGCCGCCAGGTGATCCAGAGCCCCTCGTACGTGGTCCGGGCAAACATCGGAACCCCTGCCCAGACGCTGCTATTGGCCCTGGATACCAGCAATGATGCCACGTGGATTCCGTGTTCCGGCTGCGTGGGCTGCCCGGGAACCTCCGTTTTCTCCTCTGATAAATCCTCCTCCTTCCGGCCGCTCCCCTGCCAGTCGCCGCAATGCAGCCAGGTACCGAACCCCAGTTGCGGTACCAGCGCGTGCGGTTTCAACATGACATACGGCAGCTCGACGGTGGCGGCGGATTTGGTTCAAGACAACATAACGCTGGCTAGGGACTCGGTTCCGGCGTTCACATTCGGGTGCATCACGAAGGCCACCGGGAGCTCAGTGCCGCCGCAGGGGCTGTTGGGGCTGGGCCGTGGCTCATTGTCGCTCTTGTCGCAGACCCAGAGTCTGTACCAGTCCACATTCTCCTACTGCCTTCCCAGCTTCAAATCGGCTAGCTTTTCTGGGTCGTTGCGGCTCGGGCCAGTGGCTCAGCCTAAGAGGATTAAGTATACGCCGCTCCTCCGCAACCCGAGACGGTCGTCGTTGTATTATGTCAATTTGATCGGGATTAGAGTCGGTAGTAAAGTCGTCGACATTCCTCCTAGGACGACGTTCACAAGACTAGTGGCGCCGGCGTACACGGCGGTGAGGGACGAGTTCCAGCGAAGAGTAGGCAACGCAACCGTCTCATCTCTTGGTGGCTTCGACACTTGCTACACCGTCCCCATCGTCTCCCCCACCATAACCTTCATGTTCGACGCAATGAACGTCACTCTCCCACCGGACAACTTCCTCATCCACAGCACCGCCGGATCCACCACCTGCCTCGCCATGGCCTCGGCACCCGACAATGTCAACTCTGTCCTCAACGTTATTGCCAACATGCAACAACAAAACCACCGTATCGTCTTCGACATTCCCAATTCCCGTGTCGGTGTCGCCCGCGAACCGTGCTCTTAG

Protein sequence

MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQLLSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCPGTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPRTTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS

Homology

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BLAST of Cp4.1LG09g04500 vs. ExPASy Swiss-Prot
Match: O04496 (Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1)

HSP 1 Score: 372.9 bits (956), Expect = 4.7e-102
Identity = 200/389 (51.41%), Postives = 262/389 (67.35%), Query Frame = 0


Query: 45  DGVLQMQAKDEARLQLLSSLVA---RRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLA 104
           D VL M + D  RL  LSSLVA   + +  P+ASG Q +   +YVVRA +GTP Q + + 
Sbjct: 62  DTVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQ-LHIGNYVVRAKLGTPPQLMFMV 121

Query: 105 LDTSNDATWIPCSGCVGCPGTSV-FSSDKSSSFRPLPCQSPQCSQV-----PNPSCGTSA 164
           LDTSNDA W+PCSGC GC   S  F+++ SS++  + C + QC+Q      P+ S   S 
Sbjct: 122 LDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSV 181

Query: 165 CGFNMTY-GSSTVAADLVQDNITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSL 224
           C FN +Y G S+ +A LVQD +TLA D +P F+FGCI  A+G+S+PPQGL+GLGRG +SL
Sbjct: 182 CSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSL 241

Query: 225 LSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIG 284
           +SQT SLY   FSYCLPSF+S  FSGSL+LG + QPK I+YTPLLRNPRR SLYYVNL G
Sbjct: 242 VSQTTSLYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTG 301

Query: 285 IRVGSKVVDIPP-----------------RTTFTRLVAPAYTAVRDEFQRRVGNATVSSL 344
           + VGS  V + P                  T  TR   P Y A+RDEF+++V  ++ S+L
Sbjct: 302 VSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTL 361

Query: 345 GGFDTCYTV--PIVSPTITFMFDAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLN 404
           G FDTC++     V+P IT    ++++ LP +N LIHS+AG+ TCL+MA    N N+VLN
Sbjct: 362 GAFDTCFSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLN 421

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy Swiss-Prot
Match: Q6F4N5 (Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-90
Identity = 201/434 (46.31%), Postives = 263/434 (60.60%), Query Frame = 0


Query: 13  LLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQLLSSLVARR--SD 72
           LLLLLL    AA+   L V+H + H    S  + ++ +   D+ARL  LSS  A    S 
Sbjct: 9   LLLLLLAATVAAAAAELSVYH-NVHPSSPSPLESIIALARDDDARLLFLSSKAATAGVSS 68

Query: 73  APIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCPGTSVFSSDKS 132
           AP+ASG+     PSYVVRA +G+P+Q LLLALDTS DATW  CS C  CP +S+F+   S
Sbjct: 69  APVASGQ---APPSYVVRAGLGSPSQQLLLALDTSADATWAHCSPCGTCPSSSLFAPANS 128

Query: 133 SSFRPLPCQSPQC-----SQVPNPSCGTSA---------CGFNMTYGSSTVAADLVQDNI 192
           SS+  LPC S  C        P P  G  A         C F+  +  ++  A L  D +
Sbjct: 129 SSYASLPCSSSWCPLFQGQACPAPQGGGDAAPPPATLPTCAFSKPFADASFQAALASDTL 188

Query: 193 TLARDSVPAFTFGCITKATG--SSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFK 252
            L +D++P +TFGC++  TG  +++P QGLLGLGRG ++LLSQ  SLY   FSYCLPS++
Sbjct: 189 RLGKDAIPNYTFGCVSSVTGPTTNMPRQGLLGLGRGPMALLSQAGSLYNGVFSYCLPSYR 248

Query: 253 SASFSGSLRLGP-VAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIP-------- 312
           S  FSGSLRLG    QP+ ++YTP+LRNP RSSLYYVN+ G+ VG   V +P        
Sbjct: 249 SYYFSGSLRLGAGGGQPRSVRYTPMLRNPHRSSLYYVNVTGLSVGHAWVKVPAGSFAFDA 308

Query: 313 ---------PRTTFTRLVAPAYTAVRDEFQRRVGNAT-VSSLGGFDTCYTVPIV----SP 372
                      T  TR  AP Y A+R+EF+R+V   +  +SLG FDTC+    V    +P
Sbjct: 309 ATGAGTVVDSGTVITRWTAPVYAALREEFRRQVAAPSGYTSLGAFDTCFNTDEVAAGGAP 368

Query: 373 TITFMFD-AMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFD 405
            +T   D  +++ LP +N LIHS+A    CLAMA AP NVNSV+NVIAN+QQQN R+VFD
Sbjct: 369 AVTVHMDGGVDLALPMENTLIHSSATPLACLAMAEAPQNVNSVVNVIANLQQQNIRVVFD 428

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 184.1 bits (466), Expect = 3.1e-45
Identity = 120/353 (33.99%), Postives = 170/353 (48.16%), Query Frame = 0


Query: 82  SPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCPGTS--VFSSDKSSSFRPLPCQ 141
           S  Y  R  +GTPA+ + + LDT +D  W+ C+ C  C   S  +F   KS ++  +PC 
Sbjct: 139 SGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCS 198

Query: 142 SPQCSQVPNPSCGT--SACGFNMTYG-SSTVAADLVQDNITLARDSVPAFTFGCITKATG 201
           SP C ++ +  C T    C + ++YG  S    D   + +T  R+ V     GC     G
Sbjct: 199 SPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEG 258

Query: 202 SSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYT 261
             V   GLLGLG+G LS   QT   +   FSYCL    ++S   S+  G  A  +  ++T
Sbjct: 259 LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFT 318

Query: 262 PLLRNPRRSSLYYVNLIGIRVGSKVVD------------------IPPRTTFTRLVAPAY 321
           PLL NP+  + YYV L+GI VG   V                   I   T+ TRL+ PAY
Sbjct: 319 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 378

Query: 322 TAVRDEFQRRVGNATVS---SLGGFDTCYTV----PIVSPTITFMFDAMNVTLPPDNFLI 381
            A+RD F  RVG  T+        FDTC+ +     +  PT+   F   +V+LP  N+LI
Sbjct: 379 IAMRDAF--RVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLI 438

Query: 382 HSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS 405
                   C A A         L++I N+QQQ  R+V+D+ +SRVG A   C+
Sbjct: 439 PVDTNGKFCFAFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.3e-39
Identity = 113/348 (32.47%), Postives = 160/348 (45.98%), Query Frame = 0


Query: 82  SPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCPGTS--VFSSDKSSSFRPLPCQ 141
           S  Y VR  +G+P +   + +D+ +D  W+ C  C  C   S  VF   KS S+  + C 
Sbjct: 128 SGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAKSGSYTGVSCG 187

Query: 142 SPQCSQVPNPSCGTSACGFNMTYG-SSTVAADLVQDNITLARDSVPAFTFGCITKATGSS 201
           S  C ++ N  C +  C + + YG  S     L  + +T A+  V     GC  +  G  
Sbjct: 188 SSVCDRIENSGCHSGGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVAMGCGHRNRGMF 247

Query: 202 VPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPL 261
           +   GLLG+G GS+S + Q        F YCL S +    +GSL  G  A P    + PL
Sbjct: 248 IGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVS-RGTDSTGSLVFGREALPVGASWVPL 307

Query: 262 LRNPRRSSLYYVNLIGIRVGSKVVDIP-----------------PRTTFTRLVAPAYTAV 321
           +RNPR  S YYV L G+ VG   + +P                   T  TRL   AY A 
Sbjct: 308 VRNPRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAF 367

Query: 322 RDEFQRRVGN-ATVSSLGGFDTCYT----VPIVSPTITFMF-DAMNVTLPPDNFLIHSTA 381
           RD F+ +  N    S +  FDTCY     V +  PT++F F +   +TLP  NFL+    
Sbjct: 368 RDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDD 427

Query: 382 GSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
             T C A A++P      L++I N+QQ+  ++ FD  N  VG     C
Sbjct: 428 SGTYCFAFAASPTG----LSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.1e-37
Identity = 129/416 (31.01%), Postives = 191/416 (45.91%), Query Frame = 0


Query: 22  AAASGGSLRVFHISTHSKPLSWADGVL---QMQAKDEARLQLLSSLVARRSDAPIASGRQ 81
           A+ +  SLRV H+      LS +D  +   ++  +D+AR++ + S +++ S   ++  + 
Sbjct: 58  ASNTKSSLRVVHMHGACSHLS-SDARVDHDEIIRRDQARVESIYSKLSKNSANEVSEAKS 117

Query: 82  ---------VIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVG---CPGTSVFS 141
                     + S +Y+V   IGTP   L L  DT +D TW  C  C+G         F+
Sbjct: 118 TELPAKSGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLTWTQCEPCLGSCYSQKEPKFN 177

Query: 142 SDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYG-SSTVAADLVQDNITLAR-DSVP 201
              SS+++ + C SP C      SC  S C +++ YG  S     L ++  TL   D + 
Sbjct: 178 PSSSSTYQNVSCSSPMCEDA--ESCSASNCVYSIVYGDKSFTQGFLAKEKFTLTNSDVLE 237

Query: 202 AFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSGSLRL 261
              FGC     G      GLLGLG G LSL +QT + Y + FSYCLPSF S S +G L  
Sbjct: 238 DVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYNNIFSYCLPSFTSNS-TGHLTF 297

Query: 262 GPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------TTFTR 321
           G     + +K+TP+   P   + Y +++IGI VG K + I P             T FTR
Sbjct: 298 GSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELAITPNSFSTEGAIIDSGTVFTR 357

Query: 322 LVAPAYTAVRDEFQRRVGN-ATVSSLGGFDTCYTV----PIVSPTITFMFDAMNVTLPPD 381
           L    Y  +R  F+ ++ +  + S  G FDTCY       +  PTI F F    V     
Sbjct: 358 LPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCYDFTGLDTVTYPTIAFSFAGSTVVELDG 417

Query: 382 NFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           + +      S  CLA A   D    +  +  N+QQ    +V+D+   RVG A   C
Sbjct: 418 SGISLPIKISQVCLAFAGNDD----LPAIFGNVQQTTLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of Cp4.1LG09g04500 vs. NCBI nr
Match: XP_023542384.1 (aspartyl protease AED3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 783 bits (2022), Expect = 5.38e-285
Identity = 403/421 (95.72%), Postives = 403/421 (95.72%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL
Sbjct: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------- 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPP              
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPSAFAFNTATGSGTI 300

Query: 301 ----TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360
               TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT
Sbjct: 301 IDSGTTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360

Query: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC
Sbjct: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 420

[Close]

BLAST of Cp4.1LG09g04500 vs. NCBI nr
Match: KAG6573797.1 (Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 774 bits (1998), Expect = 1.88e-281
Identity = 399/415 (96.14%), Postives = 401/415 (96.63%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFL SLLLLLL RLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL
Sbjct: 1   MNTKPSTFLLSLLLLLL-RLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSD+SSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDQSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR-----------TT 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPP            TT
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPSAFAFNTTTGSGTT 300

Query: 301 FTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVTLPPDNF 360
           FTRLVAPAYTAVR+EFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVTLPPDNF
Sbjct: 301 FTRLVAPAYTAVRNEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVTLPPDNF 360

Query: 361 LIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS 404
           LIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS
Sbjct: 361 LIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS 414

[Close]

BLAST of Cp4.1LG09g04500 vs. NCBI nr
Match: KAG7012873.1 (Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 771 bits (1991), Expect = 2.74e-280
Identity = 398/421 (94.54%), Postives = 401/421 (95.25%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFL SLLLLLL RLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL
Sbjct: 1   MNTKPSTFLLSLLLLLL-RLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSD+SSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDQSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------- 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPP              
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPSAFAFNTTTGSGTI 300

Query: 301 ----TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360
               TTFTRLVAPAYTAVR+EFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT
Sbjct: 301 IDSGTTFTRLVAPAYTAVRNEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360

Query: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRI+FDIPNSRVGVAREPC
Sbjct: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIIFDIPNSRVGVAREPC 420

[Close]

BLAST of Cp4.1LG09g04500 vs. NCBI nr
Match: XP_022945370.1 (aspartyl protease AED3-like [Cucurbita moschata])

HSP 1 Score: 766 bits (1979), Expect = 1.78e-278
Identity = 397/421 (94.30%), Postives = 398/421 (94.54%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFL SLLLLL  RLLAAASGGSLRVFHIS HSKPLSWADGVLQMQAKDEARLQL
Sbjct: 1   MNTKPSTFLLSLLLLL--RLLAAASGGSLRVFHISIHSKPLSWADGVLQMQAKDEARLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSD+SSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDQSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------- 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIP               
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPSSAFAFNTATGSGTI 300

Query: 301 ----TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360
               TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT
Sbjct: 301 IDSGTTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360

Query: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC
Sbjct: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 419

[Close]

BLAST of Cp4.1LG09g04500 vs. NCBI nr
Match: XP_022966895.1 (aspartyl protease AED3-like [Cucurbita maxima])

HSP 1 Score: 757 bits (1955), Expect = 8.09e-275
Identity = 391/421 (92.87%), Postives = 396/421 (94.06%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFLFSLLLLL  RLLAA SGGSLR+FHISTHSKPLSWAD VLQMQAKDE+RLQL
Sbjct: 1   MNTKPSTFLFSLLLLL--RLLAATSGGSLRIFHISTHSKPLSWADEVLQMQAKDESRLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSD PIASGRQVIQSPSYVVRA+IGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDVPIASGRQVIQSPSYVVRASIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSD+SSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDQSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------- 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLI IRVGSKVV+IP               
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGSKVVNIPSSAFTFNTATGSGTI 300

Query: 301 ----TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360
               TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT
Sbjct: 301 IDSGTTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360

Query: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC
Sbjct: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 419

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy TrEMBL
Match: A0A6J1G0S0 (aspartyl protease AED3-like OS=Cucurbita moschata OX=3662 GN=LOC111449626 PE=4 SV=1)

HSP 1 Score: 766 bits (1979), Expect = 8.62e-279
Identity = 397/421 (94.30%), Postives = 398/421 (94.54%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFL SLLLLL  RLLAAASGGSLRVFHIS HSKPLSWADGVLQMQAKDEARLQL
Sbjct: 1   MNTKPSTFLLSLLLLL--RLLAAASGGSLRVFHISIHSKPLSWADGVLQMQAKDEARLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSD+SSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDQSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------- 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIP               
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPSSAFAFNTATGSGTI 300

Query: 301 ----TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360
               TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT
Sbjct: 301 IDSGTTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360

Query: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC
Sbjct: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 419

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy TrEMBL
Match: A0A6J1HSV1 (aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111466462 PE=4 SV=1)

HSP 1 Score: 757 bits (1955), Expect = 3.92e-275
Identity = 391/421 (92.87%), Postives = 396/421 (94.06%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAASGGSLRVFHISTHSKPLSWADGVLQMQAKDEARLQL 60
           MNTKPSTFLFSLLLLL  RLLAA SGGSLR+FHISTHSKPLSWAD VLQMQAKDE+RLQL
Sbjct: 1   MNTKPSTFLFSLLLLL--RLLAATSGGSLRIFHISTHSKPLSWADEVLQMQAKDESRLQL 60

Query: 61  LSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120
           LSSLVARRSD PIASGRQVIQSPSYVVRA+IGTPAQTLLLALDTSNDATWIPCSGCVGCP
Sbjct: 61  LSSLVARRSDVPIASGRQVIQSPSYVVRASIGTPAQTLLLALDTSNDATWIPCSGCVGCP 120

Query: 121 GTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180
           GTSVFSSD+SSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR
Sbjct: 121 GTSVFSSDQSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLAR 180

Query: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240
           DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG
Sbjct: 181 DSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSG 240

Query: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------------- 300
           SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLI IRVGSKVV+IP               
Sbjct: 241 SLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGSKVVNIPSSAFTFNTATGSGTI 300

Query: 301 ----TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360
               TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT
Sbjct: 301 IDSGTTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVT 360

Query: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
           LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC
Sbjct: 361 LPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 419

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy TrEMBL
Match: A0A6J1GYP2 (aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1)

HSP 1 Score: 657 bits (1694), Expect = 4.32e-235
Identity = 339/438 (77.40%), Postives = 372/438 (84.93%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRL-----------LAAASGGSLRVFHISTH------SKPLSW 60
           M TK S FL SLLLLL +              AA    +L+VFHI +       SKPLSW
Sbjct: 1   MKTK-SPFLLSLLLLLSISAESHHHHHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPLSW 60

Query: 61  ADGVLQMQAKDEARLQLLSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALD 120
           AD VLQMQAKD+ARLQ LSSLVAR+S APIAS RQ+IQSP++VVRA IGTPAQTLL+ALD
Sbjct: 61  ADNVLQMQAKDQARLQFLSSLVARKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMALD 120

Query: 121 TSNDATWIPCSGCVGCPGTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTYG 180
           TSNDA WIPCSGCVGCP T+VFSSDKSSSFRPLPC+SPQC+QVPNPSC +SACGFN+TYG
Sbjct: 121 TSNDAAWIPCSGCVGCPSTTVFSSDKSSSFRPLPCRSPQCNQVPNPSCSSSACGFNLTYG 180

Query: 181 SSTVAADLVQDNITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQ 240
           SSTVAADLVQDNITLA DSVPA+TFGCITKATGSSVPPQGLLGLGRG LSLL Q+QSLY+
Sbjct: 181 SSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSLYR 240

Query: 241 STFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVD 300
           STFSYCLPSFKS  FSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLI IRVG ++VD
Sbjct: 241 STFSYCLPSFKSTGFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRIVD 300

Query: 301 IPPR-----------------TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTV 360
           IPP                  TTFTRLVAPAYTAVR+EF+RR+GNAT+SSLGGFDTCYTV
Sbjct: 301 IPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCYTV 360

Query: 361 PIVSPTITFMFDAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHR 404
           PI+SPTITFMF  MNVTLPPDNFL+HS+AG+TTCLAMA+APDNVNSVLNVIA+MQQQNHR
Sbjct: 361 PIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQNHR 420

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy TrEMBL
Match: A0A6J1JIM3 (aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=1)

HSP 1 Score: 652 bits (1682), Expect = 3.12e-233
Identity = 339/440 (77.05%), Postives = 372/440 (84.55%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLL-------------AAASGGSLRVFHISTH------SKPL 60
           M TK S FL SLLLLLL                 AA    +L+VFHI +       SKPL
Sbjct: 1   MKTK-SPFLLSLLLLLLSISAESLHHHHHPNCNAAADRSSTLQVFHIFSPCSPFRPSKPL 60

Query: 61  SWADGVLQMQAKDEARLQLLSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLA 120
           SWAD VLQMQAKD+ARLQ LSSLVA +S APIAS RQ+IQSP++VVRA IGTPAQTLL+A
Sbjct: 61  SWADNVLQMQAKDQARLQFLSSLVACKSVAPIASARQLIQSPTFVVRAKIGTPAQTLLMA 120

Query: 121 LDTSNDATWIPCSGCVGCPGTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMT 180
           LDTSNDA WIPCSGCVGCP T+VFSSDKS+SFRPLPC+SPQC+QVPNPSC +SACGFN+T
Sbjct: 121 LDTSNDAAWIPCSGCVGCPSTTVFSSDKSTSFRPLPCRSPQCNQVPNPSCSSSACGFNLT 180

Query: 181 YGSSTVAADLVQDNITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSL 240
           YGSSTVAADLVQDNITLA DSVPA+TFGCITKATGSSVPPQGLLGLGRG LSLL Q+QSL
Sbjct: 181 YGSSTVAADLVQDNITLANDSVPAYTFGCITKATGSSVPPQGLLGLGRGPLSLLGQSQSL 240

Query: 241 YQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKV 300
           Y+STFSYCLPSFKS SFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLI IRVG ++
Sbjct: 241 YRSTFSYCLPSFKSTSFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIAIRVGRRI 300

Query: 301 VDIPPR-----------------TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCY 360
           VDIPP                  TTFTRLVAPAYTAVR+EF+RR+GNAT+SSLGGFDTCY
Sbjct: 301 VDIPPSALAFNSATGAGTIIDSGTTFTRLVAPAYTAVRNEFRRRIGNATISSLGGFDTCY 360

Query: 361 TVPIVSPTITFMFDAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQN 404
           TVPI+SPTITFMF  MNVTLPPDNFL+HS+AG+TTCLAMA+APDNVNSVLNVIA+MQQQN
Sbjct: 361 TVPIISPTITFMFAGMNVTLPPDNFLLHSSAGTTTCLAMAAAPDNVNSVLNVIASMQQQN 420

[Close]

BLAST of Cp4.1LG09g04500 vs. ExPASy TrEMBL
Match: A0A0A0KTR5 (Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=4 SV=1)

HSP 1 Score: 650 bits (1678), Expect = 1.36e-232
Identity = 342/440 (77.73%), Postives = 365/440 (82.95%), Query Frame = 0


Query: 1   MNTKPSTFLFSLLLLLLLRLLAAAS------------GGSLRVFHISTH------SKPLS 60
           MN   S FL  LLLL L  +  A S              +L+VFHI +       SKPLS
Sbjct: 1   MNNTKSPFLLLLLLLHLFSISTAKSHIPSNCNPAADRSSTLQVFHIFSPCSPFRPSKPLS 60

Query: 61  WADGVLQMQAKDEARLQLLSSLVARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLAL 120
           WAD VLQMQAKD+ARLQ LSSLVARRS  PIAS RQ+IQSP++VVRA IGTPAQTLLLAL
Sbjct: 61  WADNVLQMQAKDQARLQFLSSLVARRSFVPIASARQLIQSPTFVVRAKIGTPAQTLLLAL 120

Query: 121 DTSNDATWIPCSGCVGCPGTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTSACGFNMTY 180
           DTSNDA WIPCSGC+GCP T+VFSSDKSSSFRPLPCQSPQC+QVPNPSC  SACGFN+TY
Sbjct: 121 DTSNDAAWIPCSGCIGCPSTTVFSSDKSSSFRPLPCQSPQCNQVPNPSCSGSACGFNLTY 180

Query: 181 GSSTVAADLVQDNITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLY 240
           GSSTVAADLVQDN+TLA DSVP++TFGCI KATGSSVPPQGLLGLGRG LSLL Q+QSLY
Sbjct: 181 GSSTVAADLVQDNLTLATDSVPSYTFGCIRKATGSSVPPQGLLGLGRGPLSLLGQSQSLY 240

Query: 241 QSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVV 300
           QSTFSYCLPSFKS +FSGSLRLGPVAQP RIKYTPLLRNPRRSSLYYVNLI IRVG K+V
Sbjct: 241 QSTFSYCLPSFKSVNFSGSLRLGPVAQPIRIKYTPLLRNPRRSSLYYVNLISIRVGRKIV 300

Query: 301 DIPPR-----------------TTFTRLVAPAYTAVRDEFQRRVG-NATVSSLGGFDTCY 360
           DIPP                  TTFTRLVAPAYTAVRDEF+RRVG N TVSSLGGFDTCY
Sbjct: 301 DIPPSALAFNSATGAGTVIDSGTTFTRLVAPAYTAVRDEFRRRVGRNVTVSSLGGFDTCY 360

Query: 361 TVPIVSPTITFMFDAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQN 404
           TVPI+SPTITFMF  MNVTLPPDNFLIHSTAGSTTCLAMA+APDNVNSVLNVIA+MQQQN
Sbjct: 361 TVPIISPTITFMFAGMNVTLPPDNFLIHSTAGSTTCLAMAAAPDNVNSVLNVIASMQQQN 420

[Close]

BLAST of Cp4.1LG09g04500 vs. TAIR 10
Match: AT5G07030.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 504.6 bits (1298), Expect = 7.4e-143
Identity = 254/406 (62.56%), Postives = 310/406 (76.35%), Query Frame = 0


Query: 26  GGSLRVFHIST------HSKPLSWADGVLQMQAKDEARLQLLSSLVARRSDAPIASGRQV 85
           G +LR+FHI +       S PLSW   VLQ  A+D+ARLQ LSSLVA RS  PIASGRQ+
Sbjct: 50  GSTLRIFHIDSPCSPFKSSSPLSWEARVLQTLAQDQARLQYLSSLVAGRSVVPIASGRQM 109

Query: 86  IQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCPGTSVFSSDKSSSFRPLPCQ 145
           +QS +Y+V+A IGTPAQ LLLA+DTS+D  WIPCSGCVGCP  + FS  KS+SF+ + C 
Sbjct: 110 LQSTTYIVKALIGTPAQPLLLAMDTSSDVAWIPCSGCVGCPSNTAFSPAKSTSFKNVSCS 169

Query: 146 SPQCSQVPNPSCGTSACGFNMTYGSSTVAADLVQDNITLARDSVPAFTFGCITKATGSSV 205
           +PQC QVPNP+CG  AC FN+TYGSS++AA+L QD I LA D + AFTFGC+ K  G   
Sbjct: 170 APQCKQVPNPTCGARACSFNLTYGSSSIAANLSQDTIRLAADPIKAFTFGCVNKVAGGGT 229

Query: 206 --PPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTP 265
             PPQGLLGLGRG LSL+SQ QS+Y+STFSYCLPSF+S +FSGSLRLGP +QP+R+KYT 
Sbjct: 230 IPPPQGLLGLGRGPLSLMSQAQSIYKSTFSYCLPSFRSLTFSGSLRLGPTSQPQRVKYTQ 289

Query: 266 LLRNPRRSSLYYVNLIGIRVGSKVVDIPPR-----------------TTFTRLVAPAYTA 325
           LLRNPRRSSLYYVNL+ IRVG KVVD+PP                  T +TRL  P Y A
Sbjct: 290 LLRNPRRSSLYYVNLVAIRVGRKVVDLPPAAIAFNPSTGAGTIFDSGTVYTRLAKPVYEA 349

Query: 326 VRDEFQRRV--GNATVSSLGGFDTCYTVPIVSPTITFMFDAMNVTLPPDNFLIHSTAGST 385
           VR+EF++RV    A V+SLGGFDTCY+  +  PTITFMF  +N+T+P DN ++HSTAGST
Sbjct: 350 VRNEFRKRVKPTTAVVTSLGGFDTCYSGQVKVPTITFMFKGVNMTMPADNLMLHSTAGST 409

Query: 386 TCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS 405
           +CLAMA+AP+NVNSV+NVIA+MQQQNHR++ D+PN R+G+ARE CS
Sbjct: 410 SCLAMAAAPENVNSVVNVIASMQQQNHRVLIDVPNGRLGLARERCS 455

[Close]

BLAST of Cp4.1LG09g04500 vs. TAIR 10
Match: AT3G54400.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.1e-141
Identity = 264/427 (61.83%), Postives = 321/427 (75.18%), Query Frame = 0


Query: 11  SLLLLLLLRLLAAAS----------GGSLRVFHISTHSKP----LSWADGVLQMQAKDEA 70
           S LL+LL+ LL   S             LRVFHI++   P    +SWAD +LQ    D+A
Sbjct: 3   SHLLILLISLLILKSESINCNEKSHSSDLRVFHINSLCSPFKTSVSWADTLLQ----DKA 62

Query: 71  RLQLLSSLV-ARRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLALDTSNDATWIPCSG 130
           R   LSSL   R+S  PIASGR ++QSP+Y+VRANIGTPAQ +L+ALDTSNDA WIPCSG
Sbjct: 63  RFLYLSSLAGVRKSSVPIASGRAIVQSPTYIVRANIGTPAQPMLVALDTSNDAAWIPCSG 122

Query: 131 CVGCPGTSVFSSDKSSSFRPLPCQSPQCSQVPNPSCGTS-ACGFNMTYGSSTVAADLVQD 190
           CVGC  + +F   KSSS R L C++PQC Q PNPSC  S +CGFNMTYG ST+ A L QD
Sbjct: 123 CVGCSSSVLFDPSKSSSSRTLQCEAPQCKQAPNPSCTVSKSCGFNMTYGGSTIEAYLTQD 182

Query: 191 NITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFK 250
            +TLA D +P +TFGCI KA+G+S+P QGL+GLGRG LSL+SQ+Q+LYQSTFSYCLP+ K
Sbjct: 183 TLTLASDVIPNYTFGCINKASGTSLPAQGLMGLGRGPLSLISQSQNLYQSTFSYCLPNSK 242

Query: 251 SASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIGIRVGSKVVDIPPR------- 310
           S++FSGSLRLGP  QP RIK TPLL+NPRRSSLYYVNL+GIRVG+K+VDIP         
Sbjct: 243 SSNFSGSLRLGPKNQPIRIKTTPLLKNPRRSSLYYVNLVGIRVGNKIVDIPTSALAFDPA 302

Query: 311 ----------TTFTRLVAPAYTAVRDEFQRRVGNATVSSLGGFDTCYTVPIVSPTITFMF 370
                     T +TRLV PAY AVR+EF+RRV NA  +SLGGFDTCY+  +V P++TFMF
Sbjct: 303 TGAGTIFDSGTVYTRLVEPAYVAVRNEFRRRVKNANATSLGGFDTCYSGSVVFPSVTFMF 362

Query: 371 DAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVG 405
             MNVTLPPDN LIHS+AG+ +CLAMA+AP NVNSVLNVIA+MQQQNHR++ D+PNSR+G
Sbjct: 363 AGMNVTLPPDNLLIHSSAGNLSCLAMAAAPVNVNSVLNVIASMQQQNHRVLIDVPNSRLG 422

[Close]

BLAST of Cp4.1LG09g04500 vs. TAIR 10
Match: AT1G09750.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 372.9 bits (956), Expect = 3.4e-103
Identity = 200/389 (51.41%), Postives = 262/389 (67.35%), Query Frame = 0


Query: 45  DGVLQMQAKDEARLQLLSSLVA---RRSDAPIASGRQVIQSPSYVVRANIGTPAQTLLLA 104
           D VL M + D  RL  LSSLVA   + +  P+ASG Q +   +YVVRA +GTP Q + + 
Sbjct: 62  DTVLHMASSDSHRLTYLSSLVAGKPKPTSVPVASGNQ-LHIGNYVVRAKLGTPPQLMFMV 121

Query: 105 LDTSNDATWIPCSGCVGCPGTSV-FSSDKSSSFRPLPCQSPQCSQV-----PNPSCGTSA 164
           LDTSNDA W+PCSGC GC   S  F+++ SS++  + C + QC+Q      P+ S   S 
Sbjct: 122 LDTSNDAVWLPCSGCSGCSNASTSFNTNSSSTYSTVSCSTAQCTQARGLTCPSSSPQPSV 181

Query: 165 CGFNMTY-GSSTVAADLVQDNITLARDSVPAFTFGCITKATGSSVPPQGLLGLGRGSLSL 224
           C FN +Y G S+ +A LVQD +TLA D +P F+FGCI  A+G+S+PPQGL+GLGRG +SL
Sbjct: 182 CSFNQSYGGDSSFSASLVQDTLTLAPDVIPNFSFGCINSASGNSLPPQGLMGLGRGPMSL 241

Query: 225 LSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYTPLLRNPRRSSLYYVNLIG 284
           +SQT SLY   FSYCLPSF+S  FSGSL+LG + QPK I+YTPLLRNPRR SLYYVNL G
Sbjct: 242 VSQTTSLYSGVFSYCLPSFRSFYFSGSLKLGLLGQPKSIRYTPLLRNPRRPSLYYVNLTG 301

Query: 285 IRVGSKVVDIPP-----------------RTTFTRLVAPAYTAVRDEFQRRVGNATVSSL 344
           + VGS  V + P                  T  TR   P Y A+RDEF+++V  ++ S+L
Sbjct: 302 VSVGSVQVPVDPVYLTFDANSGAGTIIDSGTVITRFAQPVYEAIRDEFRKQVNVSSFSTL 361

Query: 345 GGFDTCYTV--PIVSPTITFMFDAMNVTLPPDNFLIHSTAGSTTCLAMASAPDNVNSVLN 404
           G FDTC++     V+P IT    ++++ LP +N LIHS+AG+ TCL+MA    N N+VLN
Sbjct: 362 GAFDTCFSADNENVAPKITLHMTSLDLKLPMENTLIHSSAGTLTCLSMAGIRQNANAVLN 421

[Close]

BLAST of Cp4.1LG09g04500 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 184.1 bits (466), Expect = 2.2e-46
Identity = 120/353 (33.99%), Postives = 170/353 (48.16%), Query Frame = 0


Query: 82  SPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGCPGTS--VFSSDKSSSFRPLPCQ 141
           S  Y  R  +GTPA+ + + LDT +D  W+ C+ C  C   S  +F   KS ++  +PC 
Sbjct: 139 SGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRKSKTYATIPCS 198

Query: 142 SPQCSQVPNPSCGT--SACGFNMTYG-SSTVAADLVQDNITLARDSVPAFTFGCITKATG 201
           SP C ++ +  C T    C + ++YG  S    D   + +T  R+ V     GC     G
Sbjct: 199 SPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGVALGCGHDNEG 258

Query: 202 SSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFSGSLRLGPVAQPKRIKYT 261
             V   GLLGLG+G LS   QT   +   FSYCL    ++S   S+  G  A  +  ++T
Sbjct: 259 LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNAAVSRIARFT 318

Query: 262 PLLRNPRRSSLYYVNLIGIRVGSKVVD------------------IPPRTTFTRLVAPAY 321
           PLL NP+  + YYV L+GI VG   V                   I   T+ TRL+ PAY
Sbjct: 319 PLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVTRLIRPAY 378

Query: 322 TAVRDEFQRRVGNATVS---SLGGFDTCYTV----PIVSPTITFMFDAMNVTLPPDNFLI 381
            A+RD F  RVG  T+        FDTC+ +     +  PT+   F   +V+LP  N+LI
Sbjct: 379 IAMRDAF--RVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSLPATNYLI 438

Query: 382 HSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPCS 405
                   C A A         L++I N+QQQ  R+V+D+ +SRVG A   C+
Sbjct: 439 PVDTNGKFCFAFAGTMGG----LSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Cp4.1LG09g04500 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 171.0 bits (432), Expect = 1.9e-42
Identity = 124/358 (34.64%), Postives = 170/358 (47.49%), Query Frame = 0


Query: 82  SPSYVVRANIGTPAQTLLLALDTSNDATWIPCSGCVGC--PGTSVFSSDKSSSFRPLPCQ 141
           S  Y +R  +GTPA  + + LDT +D  W+ CS C  C     ++F   KS +F  +PC 
Sbjct: 132 SGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATVPCG 191

Query: 142 SPQCSQVPNPS-CGT---SACGFNMTYG-SSTVAADLVQDNITLARDSVPAFTFGCITKA 201
           S  C ++ + S C T     C + ++YG  S    D   + +T     V     GC    
Sbjct: 192 SRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCGHDN 251

Query: 202 TGSSVPPQGLLGLGRGSLSLLSQTQSLYQSTFSYCLPSFKSASFS----GSLRLGPVAQP 261
            G  V   GLLGLGRG LS  SQT++ Y   FSYCL    S+  S     ++  G  A P
Sbjct: 252 EGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNAAVP 311

Query: 262 KRIKYTPLLRNPRRSSLYYVNLIGIRV-GSKV-----------------VDIPPRTTFTR 321
           K   +TPLL NP+  + YY+ L+GI V GS+V                 V I   T+ TR
Sbjct: 312 KTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTSVTR 371

Query: 322 LVAPAYTAVRDEFQRRVGNATVS---SLGGFDTCYTV----PIVSPTITFMFDAMNVTLP 381
           L  PAY A+RD F  R+G   +    S   FDTC+ +     +  PT+ F F    V+LP
Sbjct: 372 LTQPAYVALRDAF--RLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSLP 431

Query: 382 PDNFLIHSTAGSTTCLAMASAPDNVNSVLNVIANMQQQNHRIVFDIPNSRVGVAREPC 404
             N+LI        C A A    +    L++I N+QQQ  R+ +D+  SRVG     C
Sbjct: 432 ASNYLIPVNTEGRFCFAFAGTMGS----LSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g04500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04496	4.7e-102	51.41	Aspartyl protease AED3 OS=Arabidopsis thaliana OX=3702 GN=AED3 PE=1 SV=1	[more]
Q6F4N5	1.4e-90	46.31	Aspartyl protease 25 OS=Oryza sativa subsp. japonica OX=39947 GN=AP25 PE=2 SV=1	[more]
Q9LNJ3	3.1e-45	33.99	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	3.3e-39	32.47	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LEW3	1.1e-37	31.01	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]

BLAST of Cp4.1LG09g04500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023542384.1	5.38e-285	95.72	aspartyl protease AED3-like [Cucurbita pepo subsp. pepo]	[more]
KAG6573797.1	1.88e-281	96.14	Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7012873.1	2.74e-280	94.54	Aspartyl protease AED3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022945370.1	1.78e-278	94.30	aspartyl protease AED3-like [Cucurbita moschata]	[more]
XP_022966895.1	8.09e-275	92.87	aspartyl protease AED3-like [Cucurbita maxima]	[more]

BLAST of Cp4.1LG09g04500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0S0	8.62e-279	94.30	aspartyl protease AED3-like OS=Cucurbita moschata OX=3662 GN=LOC111449626 PE=4 S...	[more]
A0A6J1HSV1	3.92e-275	92.87	aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111466462 PE=4 SV=...	[more]
A0A6J1GYP2	4.32e-235	77.40	aspartyl protease AED3 OS=Cucurbita moschata OX=3662 GN=LOC111458686 PE=4 SV=1	[more]
A0A6J1JIM3	3.12e-233	77.05	aspartyl protease AED3-like OS=Cucurbita maxima OX=3661 GN=LOC111486144 PE=4 SV=...	[more]
A0A0A0KTR5	1.36e-232	77.73	Aspartic proteinase nepenthesin-1 OS=Cucumis sativus OX=3659 GN=Csa_5G623870 PE=...	[more]

BLAST of Cp4.1LG09g04500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G07030.1	7.4e-143	62.56	Eukaryotic aspartyl protease family protein	[more]
AT3G54400.1	1.1e-141	61.83	Eukaryotic aspartyl protease family protein	[more]
AT1G09750.1	3.4e-103	51.41	Eukaryotic aspartyl protease family protein	[more]
AT1G01300.1	2.2e-46	33.99	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.9e-42	34.64	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 65..245 e-value: 1.8E-38 score: 134.5
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 247..404 e-value: 9.2E-36 score: 125.3
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 83..403
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 268..397 e-value: 4.6E-18 score: 65.5
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 85..245 e-value: 6.2E-38 score: 130.8
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 10..404
None	No IPR available	PANTHER	PTHR13683:SF798	ASPARTYL PROTEASE AED3-LIKE	coord: 10..404
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 85..399 score: 33.311874

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g04500.1	Cp4.1LG09g04500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity