Cp4.1LG09g04070 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g04070 vs. ExPASy Swiss-Prot
Match: Q8GTR4 (Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2)

HSP 1 Score: 1318.9 bits (3412), Expect = 0.0e+00
Identity = 617/899 (68.63%), Postives = 743/899 (82.65%), Query Frame = 0

Query: 65  SSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLRVKDG 124
           S+S+ MS+E S+  SQ  D L+Y+RA+WV++ VIAWNV+VG GSCY + SK A L   + 
Sbjct: 65  SASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSED 124

Query: 125 VVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATFNSYG 184
            ++GYD++IKLE + G LP +VI+KFPHI+NY  F VP    +  L+K QLAV  F++ G
Sbjct: 125 GIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEG 184

Query: 185 QCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGGRPFE 244
           +    T LQLPGVLD+LFSY+GPLGA ++ E VSL+LWAPTAQAV   IYK P+   P E
Sbjct: 185 RLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPME 244

Query: 245 VIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGRRTLF 304
           +  L+E +GVW T+G  SW GCYY Y+V+VYHPST+K+E C++ DPY+RG+S+DGR+T  
Sbjct: 245 ICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFL 304

Query: 305 VDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMGFTLQ 364
           V+L SDDLKPEGWD LAD+KP L SFSDISIYELHVRDFS +D++V P+ RGGY+ FT +
Sbjct: 305 VNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSK 364

Query: 365 DSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQSLIA 424
           DS G+ HL+KL +AG++H+HLLPTFQFG VDD+K  WKS+D  LLE L PDS E Q+ I 
Sbjct: 365 DSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARIT 424

Query: 425 DIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHL 484
           +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  GL VVLDVVYNHL
Sbjct: 425 EIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHL 484

Query: 485 HGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDY 544
           H  GP D  SVLDKIVPGYYLRRN DGFIENSTCVNNTASEH+MV+R+I DD+L+WVV+Y
Sbjct: 485 HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNY 544

Query: 545 KVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVN 604
           KVDGFRFDLMGH+M +T++ AK+A+  L +E +GVDGS IYLYGEGW+FGEVA+NGRG+N
Sbjct: 545 KVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGIN 604

Query: 605 ASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVEFTLA 664
           ASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HDHGS+   E  L+
Sbjct: 605 ASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLS 664

Query: 665 VSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNET 724
            +K+HIQ  MAANL+DY+LTN EGKEVKGSEV  +D + VAYAS PTET+NYVSAHDNET
Sbjct: 665 TAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNET 724

Query: 725 LFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDW 784
           LFD++SLKTP  I+V++RCR+NHLA+S+IALSQGIPFFH GDE+LRSKS+DRDSYNSGDW
Sbjct: 725 LFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDW 784

Query: 785 FNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIR 844
           FNRLDF+Y +NNWGVGLPPK KNE+NWPLIKPRL DPSFKP  SHI+A + NF +LL+IR
Sbjct: 785 FNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIR 844

Query: 845 YSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVV 904
           YSSPLFRL TA AIQ+RVRFHNTG S IPG IVMSIEDG  GIP ++Q+DPIYS IVV+ 
Sbjct: 845 YSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIF 904

Query: 905 NARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFVELR 964
           NARPSE S+P PAL+ R L+LHP+Q+MS D +VK S Y+  +G F VP+RTT+VFVE R
Sbjct: 905 NARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESR 963

BLAST of Cp4.1LG09g04070 vs. ExPASy Swiss-Prot
Match: P07206 (Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2)

HSP 1 Score: 542.3 bits (1396), Expect = 1.1e-152
Identity = 327/917 (35.66%), Postives = 490/917 (53.44%), Query Frame = 0

Query: 88   ARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLRVKDGVVEGYDVKIKLEKDRGPLPENVI 147
            A A WV +  + W          L+ S  + +   DG  +  D  +KL      + + V 
Sbjct: 184  AEAHWVDKNTLLWPGGQDKPIVRLYYSHSSKV-AADGEGKFTDRYLKLTPT--TVSQQVS 243

Query: 148  KKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATFNSYGQCNNITCLQLPGVLDDLFSYEG- 207
             +FPH+ +Y  F +P  + V+ LL+ +         G   + T +Q  GVLDD ++    
Sbjct: 244  MRFPHLSSYAAFKLPDNANVDELLQGETVAIAAAEDGILISATQVQTAGVLDDAYAEAAE 303

Query: 208  --PLGAVYSKEAVSLYLWAPTAQAVQAQIY---KEPVGGRPFEVIQLEELDGVWRTKGPK 267
                GA  +   V+  +WAPTAQ V   +Y   K+ +G  P   +  +   G W  +G  
Sbjct: 304  ALSYGAQLADGGVTFRVWAPTAQQVDVVVYSADKKVIGSHP---MTRDSASGAWSWQGGS 363

Query: 268  SWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGRRTLFVDLGSDDLKPEGWDKLA 327
              +G +Y Y +TVYHP + KVE+   TDPY+  +S++   +  VDL    LKP+GWD L 
Sbjct: 364  DLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNSEYSQVVDLNDSALKPDGWDNLT 423

Query: 328  --DEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMGFTLQDSDGITHLKKLSNAG 387
                +      + ++I+E H+RD S  DQ+V  +LRG Y+  T  DS+ + HLK LS +G
Sbjct: 424  MPHAQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAGDSNMVQHLKTLSASG 483

Query: 388  ISHVHLLPTF--------------------------------QFGGVDDDKSKWKSLDIE 447
            ++HV LLP F                                +F G  D  S  + +  +
Sbjct: 484  VTHVELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAGYCDSGSTVEEVLNQ 543

Query: 448  LLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQA 507
            L +    D+ + Q+L   +  +D YNWGY+P  + VP+GSYA+DP G  R+ EFR M+QA
Sbjct: 544  LKQSDSQDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTTRIKEFRTMIQA 603

Query: 508  LNQ-IGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRN-IDGFIENSTCVNNTASE 567
            + Q +G+ V++DVVYNH +  GP D +SVLDKIVP YY R N   G +E++TC +++A E
Sbjct: 604  IKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDSAPE 663

Query: 568  HFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIY 627
            H M  ++I D +  W  DYK+DGFRFDLMG+   + +L A   ++ L  +        IY
Sbjct: 664  HRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPD--------IY 723

Query: 628  LYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPF--GHPLQ--QGFV 687
             +GEGWD  +   + R   ASQ NL GTGIG+F+DR+RD++ GG PF  G  L+  QG  
Sbjct: 724  FFGEGWDSNQ---SDRFEIASQINLKGTGIGTFSDRLRDSVRGGGPFDSGDALRQNQGIG 783

Query: 688  TGLLLEPNDHDHGSDKEVEFTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDG 747
            +G  + PN+    SD +V        D  ++ MA NL D+V+ + +G   KGSE+  Y+G
Sbjct: 784  SGAGVLPNELASLSDDQVRHL----ADLTRLGMAGNLADFVMIDKDGAAKKGSEI-DYNG 843

Query: 748  SSVAYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPF 807
            +   YA+ PTE VNYVS HDN+TL+D++S K  +   +  R RM  ++ + + L QGI F
Sbjct: 844  APGGYAADPTEVVNYVSKHDNQTLWDMISYKASQEADLATRVRMQAVSLATVMLGQGIAF 903

Query: 808  FHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADP 867
               G ELLRSKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+ + 
Sbjct: 904  DQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRISDDGSNYEVI-TRVKEM 963

Query: 868  SFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIE 927
               P ++ +      +  L ++R SSPLF L   +A+ KRV F NTG+    G++VM+++
Sbjct: 964  VATPGEAELKQMTAFYQELTELRKSSPLFTLGDGSAVMKRVDFRNTGSDQQAGLLVMTVD 1023

Query: 928  DGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSA 959
            DG +       LD     +VV +NA P   S         TLQL  IQ  + +  + N  
Sbjct: 1024 DGMK---AGASLDSRLDGLVVAINAAPE--SRTLNEFAGETLQLSAIQQTAGENSLANGV 1072

BLAST of Cp4.1LG09g04070 vs. ExPASy Swiss-Prot
Match: P07811 (Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 5.3e-144
Identity = 308/852 (36.15%), Postives = 454/852 (53.29%), Query Frame = 0

Query: 88   ARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLRVKDGVVEGYDVKIKLEKDRGPLPENVI 147
            A A WV +  + W          L+ S  + +   D   E  D  +KL      + + V 
Sbjct: 195  ADAHWVDKTTLLWPGGENKPIVRLYYSHSSKV-AADSNGEFSDKYVKLTPT--TVNQQVS 254

Query: 148  KKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATFNSYGQCNNITCLQLPGV---------- 207
             +FPH+ +Y  F +P    V+ LL+            + + I  L  PG           
Sbjct: 255  MRFPHLASYPAFKLPDDVNVDELLQ-----GDDGGIAESDGILSLSHPGADRRRAGRYLC 314

Query: 208  -LDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIY---KEPVGGRPFEVIQLEELDG 267
               +  SY    GA  +   V+  +WAPTAQ V+  IY   K+ +   P   +  +   G
Sbjct: 315  RRAEALSY----GAQLTDSGVTFRVWAPTAQQVELVIYSADKKVIASHP---MTRDSASG 374

Query: 268  VWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGRRTLFVDLGSDDLK 327
             W  +G    +G +Y Y +TVYHP + KVE+   TDPY+  +S++   +  VDL    LK
Sbjct: 375  AWSWQGGSDLKGAFYRYAMTVYHPQSRKVEQYEVTDPYAHSLSTNSEYSQVVDLNDSALK 434

Query: 328  PEGWDKLA--DEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMGFTLQDSDGITH 387
            PEGWD L     +      + ++I+E H+RD S  DQ+V  +LRG Y+  T Q+S+ + H
Sbjct: 435  PEGWDGLTMPHAQKTKADLAKMTIHESHIRDLSAWDQTVPAELRGKYLALTAQESNMVQH 494

Query: 388  LKKLSNAGISHVHLLPTF--------------------------------QFGGVDDDKS 447
            LK+LS +G++H+ LLP F                                +F G  D  S
Sbjct: 495  LKQLSASGVTHIELLPVFDLATVNEFSDKVADIQQPFSRLCEVNSAVKSSEFAGYCDSGS 554

Query: 448  KWKSLDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLI 507
              + +  +L +    D+ + Q+L   +  +D YNWGY+P  + VP+GSYA+DP G  R+ 
Sbjct: 555  TVEEVLTQLKQNDSKDNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTARIK 614

Query: 508  EFRKMVQALNQ-IGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRN-IDGFIENST 567
            EFR M+QA+ Q +G+ V++DVVYNH +  GP D +SVLDKIVP YY R N   G +E++T
Sbjct: 615  EFRTMIQAIKQDLGMNVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESAT 674

Query: 568  CVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKN 627
            C +++A EH M  ++I D +  W  DYK+DGFRFDLMG+   + +L A   ++ L  +  
Sbjct: 675  CCSDSAPEHRMFAKLIADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPD-- 734

Query: 628  GVDGSSIYLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPF--GHP 687
                  IY +GEGWD  +   + R   ASQ NL GTGIG+F+DR+RDA+ GG PF  G  
Sbjct: 735  ------IYFFGEGWDSNQ---SDRFEIASQINLKGTGIGTFSDRLRDAVRGGGPFDSGDA 794

Query: 688  LQ--QGFVTGLLLEPNDHDHGSDKEVEFTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKG 747
            L+  QG  +G  + PN+    SD +         D  ++ MA NL D+VL + +G   +G
Sbjct: 795  LRQNQGVGSGAGVLPNELTTLSDDQARHL----ADLTRLGMAGNLADFVLIDKDGAVKRG 854

Query: 748  SEVRTYDGSSVAYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSII 807
            SE+  Y+G+   YA+ PTE VNYVS HDN+TL+D++S K  +   ++ R RM  ++ + +
Sbjct: 855  SEI-DYNGAPGGYAADPTEVVNYVSKHDNQTLWDMISYKAAQEADLDTRVRMQAVSLATV 914

Query: 808  ALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPL 867
             L QGI F   G ELLRSKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +
Sbjct: 915  MLGQGIAFDQQGSELLRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRSSDDGSNYDI 974

Query: 868  IKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIP 886
            I  R+ D    P ++ +      +  L  +R SSPLF L     + KRV F NTG     
Sbjct: 975  I-ARVKDAVATPGETELKQMTAFYQELTALRKSSPLFTLGDGATVMKRVDFRNTGADQQT 1014

BLAST of Cp4.1LG09g04070 vs. ExPASy Swiss-Prot
Match: O33840 (Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2)

HSP 1 Score: 281.6 bits (719), Expect = 3.4e-74
Identity = 211/734 (28.75%), Postives = 336/734 (45.78%), Query Frame = 0

Query: 197 VLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGGRPFEVIQLE-ELDGVW 256
           +LDD + Y+G LGAVYS E     +W+P ++ V+  ++K      P++V+ +E + +GVW
Sbjct: 215 ILDDYY-YDGELGAVYSPEKTIFRVWSPVSKWVKVLLFKNGEDTEPYQVVNMEYKGNGVW 274

Query: 257 RTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGRRTLFVDLGSDDLKPE 316
                   +G +Y Y++  Y      V      DPYS+ V ++ +++  V+L   +  PE
Sbjct: 275 EAVVEGDLDGVFYLYQLENYGKIRTTV------DPYSKAVYANSKKSAVVNLARTN--PE 334

Query: 317 GWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMGFTLQDSDG----ITH 376
           GW+   D  P +  + D  IYE+H+ D +  + S   + +G Y+G T +++ G     T 
Sbjct: 335 GWEN--DRGPKIEGYEDAIIYEIHIADITGLENSGVKN-KGLYLGLTEENTKGPGGVTTG 394

Query: 377 LKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQSLIADIQNSDG 436
           L  L   G++HVH+LP F F   D+       LD +  EK                    
Sbjct: 395 LSHLVELGVTHVHILPFFDFYTGDE-------LDKD-FEKY------------------- 454

Query: 437 YNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCGPF 496
           YNWGY+P L+ VP+G Y++DP  P  R+ E ++MV+AL++ G+ V++D+V+ H +G G  
Sbjct: 455 YNWGYDPYLFMVPEGRYSTDPKNPHTRIREVKEMVKALHKHGIGVIMDMVFPHTYGIGEL 514

Query: 497 DPSSVLDKIVPGYYLRRNIDG-FIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGF 556
              S  D+ VP Y+ R +  G ++  S C N  ASE  M+ + IVD + +WV +Y +DGF
Sbjct: 515 ---SAFDQTVPYYFYRIDKTGAYLNESGCGNVIASERPMMRKFIVDTVTYWVKEYHIDGF 574

Query: 557 RFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFN 616
           RFD MG +   TML+ + AL  +          +I LYGE W        G  +   + +
Sbjct: 575 RFDQMGLIDKKTMLEVERALHKI--------DPTIILYGEPW-----GGWGAPIRFGKSD 634

Query: 617 LFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVEFTLAVSKDH 676
           + GT + +FND  RDA + GS F +P  +GFV G          G  KE +    V    
Sbjct: 635 VAGTHVAAFNDEFRDA-IRGSVF-NPSVKGFVMG----------GYGKETKIKRGVVGS- 694

Query: 677 IQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFD-- 736
                          N++GK +K            ++A  P ET+NY + HDN TL+D  
Sbjct: 695 --------------INYDGKLIK------------SFALDPEETINYAACHDNHTLWDKN 754

Query: 737 --VVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWF 796
                    +  T  +      LA +I+  SQG+PF H G +  R+K+ + +SYN+    
Sbjct: 755 YLAAKADKKKEWTEEELKNAQKLAGAILLTSQGVPFLHGGQDFCRTKNFNDNSYNAPISI 805

Query: 797 NRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIRY 856
           N  D+                                    K   +        L+++R 
Sbjct: 815 NGFDY----------------------------------ERKLQFIDVFNYHKGLIKLRK 805

Query: 857 SSPLFRLKTANAIQKRVRFHNTGTSLIPG---VIVMSIEDGQEGIPGLTQLDPIYSYIVV 916
             P FRLK A  I+K + F       +PG   ++   ++D   G P        +  IVV
Sbjct: 875 EHPAFRLKNAEEIKKHLEF-------LPGGRRIVAFMLKDHAGGDP--------WKDIVV 805

BLAST of Cp4.1LG09g04070 vs. ExPASy Swiss-Prot
Match: C0SPA0 (Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 3.3e-69
Identity = 217/697 (31.13%), Postives = 318/697 (45.62%), Query Frame = 0

Query: 190 TCLQLPGVL-----DDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGGRPFE 249
           T LQ+  V+     DD F Y+G LGAVY+ +     +WAP A +   ++      GR F+
Sbjct: 83  TDLQIGAVIRTAAFDDEFYYDGELGAVYTADHTVFKVWAPAATSAAVKLSHPNKSGRTFQ 142

Query: 250 VIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGRRTLF 309
           + +LE+  GV+         G  YEY   + + S    E   + D Y++ V+ +G + + 
Sbjct: 143 MTRLEK--GVYAVTVTGDLHG--YEYLFCICNNS----EWMETVDQYAKAVTVNGEKGVV 202

Query: 310 VDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMGFTLQ 369
                  L+P+     A  KP  H   D  IYE H+RDFSI + S   + +G Y+  T  
Sbjct: 203 -------LRPDQMKWTAPLKPFSHPV-DAVIYETHLRDFSIHENSGMIN-KGKYLALTET 262

Query: 370 D-------SDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSA 429
           D       S G+ ++K+L   G++HV LLP   F GVD++K                   
Sbjct: 263 DTQTANGSSSGLAYVKEL---GVTHVELLPVNDFAGVDEEKP------------------ 322

Query: 430 EQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVV 489
                       D YNWGYNP+ +  P+GSYAS+P+ P  R  E ++M+  L+Q GLRV+
Sbjct: 323 -----------LDAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVI 382

Query: 490 LDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCV-NNTASEHFMVERMIVD 549
           LDVV+NH++       +S  +K VPGY+ R +  G   N T V N+ ASE  M  + I D
Sbjct: 383 LDVVFNHVYK----RENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIAD 442

Query: 550 DILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGE 609
            +++W+ +Y VDGFRFDL+G L   T+L  K   +  T+ K G     I L+GEGWD   
Sbjct: 443 CVVYWLEEYNVDGFRFDLLGILDIDTVLYMK---EKATKAKPG-----ILLFGEGWDLAT 502

Query: 610 VAKNGRG---VNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHD 669
              + +     NA +      GIG FND  RDA+ G +   H    GF  G        +
Sbjct: 503 PLPHEQKAALANAPRM----PGIGFFNDMFRDAVKGNT--FHLKATGFALG--------N 562

Query: 670 HGSDKEVEFTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTE 729
             S + V   +A S     +A                                    P++
Sbjct: 563 GESAQAVMHGIAGSSGWKALA-------------------------------PIVPEPSQ 622

Query: 730 TVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSK 789
           ++NYV +HDN T +D +S   P+      R R   LA +II L+QG+PF H G E  R+K
Sbjct: 623 SINYVESHDNHTFWDKMSFALPQENDSRKRSR-QRLAAAIILLAQGVPFIHSGQEFFRTK 665

Query: 790 SMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK-------- 849
               +SY S D  N+LD       W      KE   Y   LI  R A P+F+        
Sbjct: 683 QGVENSYQSSDSINQLD-------WDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQ 665

BLAST of Cp4.1LG09g04070 vs. NCBI nr
Match: XP_022945721.1 (pullulanase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1952 bits (5056), Expect = 0.0
Identity = 949/963 (98.55%), Postives = 955/963 (99.17%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAV SS  VGGVFS+AATSLSHYP GHSRCG
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFSVAATSLSHYPHGHSRCG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCSSSASMS+EEST ASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR
Sbjct: 61  PLYCSSSASMSLEESTPASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           V DGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF
Sbjct: 121 VNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
           RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPD+RGGYMG
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGYMG 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ
Sbjct: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHG+DK+ E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
           FTLAVSKDHIQVAMAANL+DYVL NFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH
Sbjct: 661 FTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960

Query: 961 ELR 963
           ELR
Sbjct: 961 ELR 963

BLAST of Cp4.1LG09g04070 vs. NCBI nr
Match: KAG6573853.1 (Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1945 bits (5039), Expect = 0.0
Identity = 946/963 (98.23%), Postives = 952/963 (98.86%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAV SS  VGGVFS+AATSLSHYPDGHSRCG
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFSVAATSLSHYPDGHSRCG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCSSSASMS+EEST ASQLEDSLLYARAFWVSRYVIAWNVEV SGSCYLFGSKMATLR
Sbjct: 61  PLYCSSSASMSLEESTPASQLEDSLLYARAFWVSRYVIAWNVEVRSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           V DGVVEGYDVKIKLEKD GPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF
Sbjct: 121 VNDGVVEGYDVKIKLEKDSGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAVQAQIYKEPVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEVVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
           RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPD+RGGYMG
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGYMG 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ
Sbjct: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGCGPFDP SVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPFDPISVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHG+DK+ E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
           FTLAVSKDHIQVAMAANL+DYVL NFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH
Sbjct: 661 FTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960

Query: 961 ELR 963
           ELR
Sbjct: 961 ELR 963

BLAST of Cp4.1LG09g04070 vs. NCBI nr
Match: XP_022966985.1 (LOW QUALITY PROTEIN: pullulanase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1827 bits (4732), Expect = 0.0
Identity = 898/963 (93.25%), Postives = 909/963 (94.39%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MSFLFSSSLHLLPPSNPATSF H+LPPRIAAVPSS P GG+FS+AATSLSHYPDGHSRCG
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFRHNLPPRIAAVPSSPPAGGIFSVAATSLSHYPDGHSRCG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR
Sbjct: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
           RPFEVIQLEELDGVWRTKGPKSW+GCYYEYEVTVYHPSTLKVEKCFSTDPYSRG+SSDG 
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWDGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGLSSDGG 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           F+LQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ
Sbjct: 361 FSLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGCGP DPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPLDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILT EKNGVDGSSIYLYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTLEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHG+DK+ E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
           FTLA+SKDHIQVAMAANLRDYVLTNFEGKEVKGSE+RTYDGSSVAY              
Sbjct: 661 FTLAMSKDHIQVAMAANLRDYVLTNFEGKEVKGSELRTYDGSSVAYXI------------ 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
              TLF                            L +GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 ---TLF----------------------------LFKGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARPSEISFPCP LRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 920

Query: 961 ELR 963
           ELR
Sbjct: 961 ELR 920

BLAST of Cp4.1LG09g04070 vs. NCBI nr
Match: XP_038892369.1 (pullulanase 1, chloroplastic [Benincasa hispida] >XP_038892370.1 pullulanase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1770 bits (4585), Expect = 0.0
Identity = 857/963 (88.99%), Postives = 907/963 (94.18%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MS LFSSSLHLLP S PATS  +   PRIAA  SS P GG FS++ATSLSH+ D + R G
Sbjct: 1   MSLLFSSSLHLLPSSTPATSICYQAHPRIAAFSSSPPAGGFFSVSATSLSHFLDDNFRSG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
            LYCSSS+SMS+EESTS SQLEDSLLY+RAFWVSRYVIAWNVEVGSGSCYLF SKMATLR
Sbjct: 61  SLYCSSSSSMSLEESTSTSQLEDSLLYSRAFWVSRYVIAWNVEVGSGSCYLFASKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           ++DGVVEGYDVK+KLEKDRG LPENVIKKFPHIQNYCPFTVPL S VE LLKCQLAVATF
Sbjct: 121 IEDGVVEGYDVKLKLEKDRGRLPENVIKKFPHIQNYCPFTVPLASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYG+CNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAV+AQI+++PVGG
Sbjct: 181 NSYGECNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
            PFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTL++EKCF+TDPYSRG+SSDGR
Sbjct: 241 TPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQIEKCFTTDPYSRGISSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVD+GS+DL P+GWDKLADEK P+ SFSDISIYELHVRDFSISDQSVHPDLRGG+M 
Sbjct: 301 RTLFVDIGSNDLIPKGWDKLADEKHPVDSFSDISIYELHVRDFSISDQSVHPDLRGGFMA 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDS GI HLKKLSNAGISHVHLLPTFQFGGVDDDK+KWK  D ELLE LPPDSAEQ+
Sbjct: 361 FTLQDSAGINHLKKLSNAGISHVHLLPTFQFGGVDDDKTKWKFFDTELLEDLPPDSAEQE 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADI NSDGYNWGYNPILWGVPKGSYASDPNGPCR+IEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADILNSDGYNWGYNPILWGVPKGSYASDPNGPCRVIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGHGPFDPNSVLDKIVPGYYLRRNADGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHG+DK  E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKVAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
             LAVSKDHI+VAMAANLRDYVLTNFEG+EVKG EV T+DGS VAYASCP+ETVNYVSAH
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYVLTNFEGQEVKGLEVLTHDGSPVAYASCPSETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFD+VSLKTPRNI V+DRCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTPRNIAVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTY TNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSK+HILAAVEN TNL
Sbjct: 781 SGDWFNRLDFTYTTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKTHILAAVENCTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKT+NAIQ+RVRFHN+G SLIPG+IVMSIEDG EGIPGL+QLD IYSYI
Sbjct: 841 LQIRYSSPLFRLKTSNAIQRRVRFHNSGASLIPGLIVMSIEDGHEGIPGLSQLDSIYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARP+EISFPCPALRARTLQLHPIQLMSTDP+VKNS YEPSTGCFMVP RTT+VFV
Sbjct: 901 VVVVNARPTEISFPCPALRARTLQLHPIQLMSTDPIVKNSTYEPSTGCFMVPPRTTAVFV 960

Query: 961 ELR 963
           E R
Sbjct: 961 EPR 963

BLAST of Cp4.1LG09g04070 vs. NCBI nr
Match: XP_004142505.1 (pullulanase 1, chloroplastic isoform X1 [Cucumis sativus])

HSP 1 Score: 1717 bits (4448), Expect = 0.0
Identity = 839/963 (87.12%), Postives = 888/963 (92.21%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MS LFSSSLH  PPS   TS  + L    AA  SS P  G      TSLS     + R G
Sbjct: 1   MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPPAAG------TSLSPGFRHNFRTG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCS S+SMS+EESTS SQLEDSLLY+RAFWVS++VIAWNVEV SGSCYLF SKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           V+DGVVEGYDVKI+LEKDR  LPENVIKKFPHIQNYCPFTVP  S VE LLKCQLAVATF
Sbjct: 121 VEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSLYLWAPTAQAV+AQI+++PVGG
Sbjct: 181 NSYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
            PFEVI LEE+DG+WRTKGPKSW+GCYYEYEVTVYHPSTL+VEKCF+TDPYSRGVSSDGR
Sbjct: 241 MPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDL SDDL P+GWDKLADEKPP+ SFSDISIYELHVRDFSISDQSVHPDLRGGYM 
Sbjct: 301 RTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDS GI HLKKLSNAG+SHVHLLPTFQFGGVDDDK+KWK +D ELLE LPPDSAEQQ
Sbjct: 361 FTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM STMLKAK+AL+ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHG+ +  E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTHEVAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
             LAVSKDHI+VAMAANLRDY+LTNFEGKEVKG EV T+D S VAYASCPTETVNYVSAH
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFD+VSLKTPRNITV+DRCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKT+NAIQKRVRFHN+GTSLIPG+IVMSIEDG  GIPGL+QLD  YSYI
Sbjct: 841 LQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGHNGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCFMVP RTTSVFV
Sbjct: 901 VVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEPSTGCFMVPPRTTSVFV 957

Query: 961 ELR 963
           E R
Sbjct: 961 EPR 957

BLAST of Cp4.1LG09g04070 vs. ExPASy TrEMBL
Match: A0A6J1G1P3 (pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 SV=1)

HSP 1 Score: 1952 bits (5056), Expect = 0.0
Identity = 949/963 (98.55%), Postives = 955/963 (99.17%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAV SS  VGGVFS+AATSLSHYP GHSRCG
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVSSSPAVGGVFSVAATSLSHYPHGHSRCG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCSSSASMS+EEST ASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR
Sbjct: 61  PLYCSSSASMSLEESTPASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           V DGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF
Sbjct: 121 VNDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
           RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPD+RGGYMG
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDMRGGYMG 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ
Sbjct: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHG+DK+ E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
           FTLAVSKDHIQVAMAANL+DYVL NFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH
Sbjct: 661 FTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960

Query: 961 ELR 963
           ELR
Sbjct: 961 ELR 963

BLAST of Cp4.1LG09g04070 vs. ExPASy TrEMBL
Match: A0A6J1HT34 (LOW QUALITY PROTEIN: pullulanase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466529 PE=4 SV=1)

HSP 1 Score: 1827 bits (4732), Expect = 0.0
Identity = 898/963 (93.25%), Postives = 909/963 (94.39%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MSFLFSSSLHLLPPSNPATSF H+LPPRIAAVPSS P GG+FS+AATSLSHYPDGHSRCG
Sbjct: 1   MSFLFSSSLHLLPPSNPATSFRHNLPPRIAAVPSSPPAGGIFSVAATSLSHYPDGHSRCG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR
Sbjct: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG
Sbjct: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
           RPFEVIQLEELDGVWRTKGPKSW+GCYYEYEVTVYHPSTLKVEKCFSTDPYSRG+SSDG 
Sbjct: 241 RPFEVIQLEELDGVWRTKGPKSWDGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGLSSDGG 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG
Sbjct: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           F+LQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ
Sbjct: 361 FSLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHGCGP DPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGCGPLDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILT EKNGVDGSSIYLYGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTLEKNGVDGSSIYLYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHG+DK+ E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
           FTLA+SKDHIQVAMAANLRDYVLTNFEGKEVKGSE+RTYDGSSVAY              
Sbjct: 661 FTLAMSKDHIQVAMAANLRDYVLTNFEGKEVKGSELRTYDGSSVAYXI------------ 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
              TLF                            L +GIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 ---TLF----------------------------LFKGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI
Sbjct: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARPSEISFPCP LRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV
Sbjct: 901 VVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 920

Query: 961 ELR 963
           ELR
Sbjct: 961 ELR 920

BLAST of Cp4.1LG09g04070 vs. ExPASy TrEMBL
Match: A0A0A0KTI2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1)

HSP 1 Score: 1717 bits (4448), Expect = 0.0
Identity = 839/963 (87.12%), Postives = 888/963 (92.21%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MS LFSSSLH  PPS   TS  + L    AA  SS P  G      TSLS     + R G
Sbjct: 1   MSLLFSSSLHFFPPSAFPTSLCYQLHHPFAAFSSSPPAAG------TSLSPGFRHNFRTG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCS S+SMS+EESTS SQLEDSLLY+RAFWVS++VIAWNVEV SGSCYLF SKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSKHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           V+DGVVEGYDVKI+LEKDR  LPENVIKKFPHIQNYCPFTVP  S VE LLKCQLAVATF
Sbjct: 121 VEDGVVEGYDVKIRLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           NSYG+C NITCLQLPGVLDDLFSYEGPLGA+YSKEAVSLYLWAPTAQAV+AQI+++PVGG
Sbjct: 181 NSYGECKNITCLQLPGVLDDLFSYEGPLGAIYSKEAVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
            PFEVI LEE+DG+WRTKGPKSW+GCYYEYEVTVYHPSTL+VEKCF+TDPYSRGVSSDGR
Sbjct: 241 MPFEVIPLEEVDGIWRTKGPKSWKGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDL SDDL P+GWDKLADEKPP+ SFSDISIYELHVRDFSISDQSVHPDLRGGYM 
Sbjct: 301 RTLFVDLFSDDLIPKGWDKLADEKPPVDSFSDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDS GI HLKKLSNAG+SHVHLLPTFQFGGVDDDK+KWK +D ELLE LPPDSAEQQ
Sbjct: 361 FTLQDSAGINHLKKLSNAGLSHVHLLPTFQFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM STMLKAK+AL+ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDHDHG+ +  E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHDHGTHEVAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
             LAVSKDHI+VAMAANLRDY+LTNFEGKEVKG EV T+D S VAYASCPTETVNYVSAH
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFD+VSLKTPRNITV+DRCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSSPLFRLKT+NAIQKRVRFHN+GTSLIPG+IVMSIEDG  GIPGL+QLD  YSYI
Sbjct: 841 LQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGHNGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVVVNARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCFMVP RTTSVFV
Sbjct: 901 VVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEPSTGCFMVPPRTTSVFV 957

Query: 961 ELR 963
           E R
Sbjct: 961 EPR 957

BLAST of Cp4.1LG09g04070 vs. ExPASy TrEMBL
Match: A0A5A7SZ60 (Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 PE=4 SV=1)

HSP 1 Score: 1715 bits (4441), Expect = 0.0
Identity = 837/963 (86.92%), Postives = 884/963 (91.80%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MS LFSSSLH  P     TS  + L PR AA  SS P  G+FS A TSLSH    + R G
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSAAGTSLSHGSHHNFRTG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCS S+SMS+EESTS SQLEDSLLY+RAFWVSR+VIAWNVEV SGSCYLF SKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           VKDGVVEGYDVKI LEKDR  LPENVIKKFPHIQNYCPFTVP  S VE LLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           +SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+++PVGG
Sbjct: 181 DSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
            PFEVI LEE+DGVWRTKGPKSWEGCYYEYEVTVYHPSTL+VEKCF+TDPYSRGVSSDGR
Sbjct: 241 MPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDL SDDL P+GWDKLAD+KPP+ SF+DISIYELHVRDFSISDQSVHPDLRGGYM 
Sbjct: 301 RTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDS GI HLK LSNAG+SHVHLLPTF FGGVDDDK+KWK +D ELLE LPPDSAEQQ
Sbjct: 361 FTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLH  GP D +SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDH HG+ +  E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEVAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
             LAVSKDHI+VAMAANLRDY+LTNFEGKEVKG EV T+D S VAYASCPTETVNYVSAH
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFD+VSLKT RNITV+DRCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSS LFRLKT++AIQKRVRFHN+GTSLIPG+IVMSIEDG EGIPGL+QLD  YSYI
Sbjct: 841 LQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVV NARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCF+VP RTTSVFV
Sbjct: 901 VVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSVFV 960

Query: 961 ELR 963
           E R
Sbjct: 961 EPR 963

BLAST of Cp4.1LG09g04070 vs. ExPASy TrEMBL
Match: A0A1S3BGM5 (pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1)

HSP 1 Score: 1715 bits (4441), Expect = 0.0
Identity = 837/963 (86.92%), Postives = 884/963 (91.80%), Query Frame = 0

Query: 1   MSFLFSSSLHLLPPSNPATSFSHHLPPRIAAVPSSHPVGGVFSLAATSLSHYPDGHSRCG 60
           MS LFSSSLH  P     TS  + L PR AA  SS P  G+FS A TSLSH    + R G
Sbjct: 1   MSLLFSSSLHFFPSLASPTSLRYQLHPRFAASSSSPPAAGLFSAAGTSLSHGSHHNFRTG 60

Query: 61  PLYCSSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLR 120
           PLYCS S+SMS+EESTS SQLEDSLLY+RAFWVSR+VIAWNVEV SGSCYLF SKMATLR
Sbjct: 61  PLYCSPSSSMSLEESTSTSQLEDSLLYSRAFWVSRHVIAWNVEVESGSCYLFASKMATLR 120

Query: 121 VKDGVVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATF 180
           VKDGVVEGYDVKI LEKDR  LPENVIKKFPHIQNYCPFTVP  S VE LLKCQLAVATF
Sbjct: 121 VKDGVVEGYDVKITLEKDRSQLPENVIKKFPHIQNYCPFTVPPASDVEALLKCQLAVATF 180

Query: 181 NSYGQCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGG 240
           +SYG+C NITCLQLPGVLDDLFSYEGPLGAVYSKE VSLYLWAPTAQAV+AQI+++PVGG
Sbjct: 181 DSYGECKNITCLQLPGVLDDLFSYEGPLGAVYSKEGVSLYLWAPTAQAVRAQIFRDPVGG 240

Query: 241 RPFEVIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGR 300
            PFEVI LEE+DGVWRTKGPKSWEGCYYEYEVTVYHPSTL+VEKCF+TDPYSRGVSSDGR
Sbjct: 241 MPFEVIPLEEVDGVWRTKGPKSWEGCYYEYEVTVYHPSTLQVEKCFTTDPYSRGVSSDGR 300

Query: 301 RTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMG 360
           RTLFVDL SDDL P+GWDKLAD+KPP+ SF+DISIYELHVRDFSISDQSVHPDLRGGYM 
Sbjct: 301 RTLFVDLCSDDLIPKGWDKLADDKPPVDSFTDISIYELHVRDFSISDQSVHPDLRGGYMA 360

Query: 361 FTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQ 420
           FTLQDS GI HLK LSNAG+SHVHLLPTF FGGVDDDK+KWK +D ELLE LPPDSAEQQ
Sbjct: 361 FTLQDSAGINHLKNLSNAGLSHVHLLPTFHFGGVDDDKTKWKFVDTELLENLPPDSAEQQ 420

Query: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVV 480
           SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKMVQALNQIGLRVVLDVV
Sbjct: 421 SLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKMVQALNQIGLRVVLDVV 480

Query: 481 YNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHW 540
           YNHLH  GP D +SVLDKIVPGYYLRRN DGFIENSTCVNNTASEHFMVERMIVDDILHW
Sbjct: 481 YNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTASEHFMVERMIVDDILHW 540

Query: 541 VVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNG 600
           VVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSIY+YGEGWDFGEVAKNG
Sbjct: 541 VVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSIYIYGEGWDFGEVAKNG 600

Query: 601 RGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVE 660
           RGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGLLLEPNDH HG+ +  E
Sbjct: 601 RGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGLLLEPNDHHHGTHEVAE 660

Query: 661 FTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAH 720
             LAVSKDHI+VAMAANLRDY+LTNFEGKEVKG EV T+D S VAYASCPTETVNYVSAH
Sbjct: 661 SMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPVAYASCPTETVNYVSAH 720

Query: 721 DNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780
           DNETLFD+VSLKT RNITV+DRCR+NHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN
Sbjct: 721 DNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYN 780

Query: 781 SGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840
           SGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFKPSKSHILAAVENFTNL
Sbjct: 781 SGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFKPSKSHILAAVENFTNL 840

Query: 841 LQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYI 900
           LQIRYSS LFRLKT++AIQKRVRFHN+GTSLIPG+IVMSIEDG EGIPGL+QLD  YSYI
Sbjct: 841 LQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGHEGIPGLSQLDSTYSYI 900

Query: 901 VVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFV 960
           VVV NARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEPSTGCF+VP RTTSVFV
Sbjct: 901 VVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEPSTGCFVVPPRTTSVFV 960

Query: 961 ELR 963
           E R
Sbjct: 961 EPR 963

BLAST of Cp4.1LG09g04070 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 1318.9 bits (3412), Expect = 0.0e+00
Identity = 617/899 (68.63%), Postives = 743/899 (82.65%), Query Frame = 0

Query: 65  SSSASMSVEESTSASQLEDSLLYARAFWVSRYVIAWNVEVGSGSCYLFGSKMATLRVKDG 124
           S+S+ MS+E S+  SQ  D L+Y+RA+WV++ VIAWNV+VG GSCY + SK A L   + 
Sbjct: 65  SASSPMSLEVSSPNSQFLDCLIYSRAYWVTQGVIAWNVDVGEGSCYFYASKSAGLSFSED 124

Query: 125 VVEGYDVKIKLEKDRGPLPENVIKKFPHIQNYCPFTVPLGSAVEPLLKCQLAVATFNSYG 184
            ++GYD++IKLE + G LP +VI+KFPHI+NY  F VP    +  L+K QLAV  F++ G
Sbjct: 125 GIDGYDLRIKLEAESGSLPADVIEKFPHIRNYKSFKVPKDLDIRDLVKSQLAVVCFDAEG 184

Query: 185 QCNNITCLQLPGVLDDLFSYEGPLGAVYSKEAVSLYLWAPTAQAVQAQIYKEPVGGRPFE 244
           +    T LQLPGVLD+LFSY+GPLGA ++ E VSL+LWAPTAQAV   IYK P+   P E
Sbjct: 185 RLIQGTGLQLPGVLDELFSYDGPLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLDKSPME 244

Query: 245 VIQLEELDGVWRTKGPKSWEGCYYEYEVTVYHPSTLKVEKCFSTDPYSRGVSSDGRRTLF 304
           +  L+E +GVW T+G  SW GCYY Y+V+VYHPST+K+E C++ DPY+RG+S+DGR+T  
Sbjct: 245 ICPLKEANGVWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYANDPYARGLSADGRKTFL 304

Query: 305 VDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDFSISDQSVHPDLRGGYMGFTLQ 364
           V+L SDDLKPEGWD LAD+KP L SFSDISIYELHVRDFS +D++V P+ RGGY+ FT +
Sbjct: 305 VNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPENRGGYLAFTSK 364

Query: 365 DSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWKSLDIELLEKLPPDSAEQQSLIA 424
           DS G+ HL+KL +AG++H+HLLPTFQFG VDD+K  WKS+D  LLE L PDS E Q+ I 
Sbjct: 365 DSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRPDSTEAQARIT 424

Query: 425 DIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHL 484
           +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKMVQALN  GL VVLDVVYNHL
Sbjct: 425 EIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKMVQALNCTGLNVVLDVVYNHL 484

Query: 485 HGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDY 544
           H  GP D  SVLDKIVPGYYLRRN DGFIENSTCVNNTASEH+MV+R+I DD+L+WVV+Y
Sbjct: 485 HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHYMVDRLIRDDLLNWVVNY 544

Query: 545 KVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVN 604
           KVDGFRFDLMGH+M +T++ AK+A+  L +E +GVDGS IYLYGEGW+FGEVA+NGRG+N
Sbjct: 545 KVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRIYLYGEGWNFGEVAENGRGIN 604

Query: 605 ASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVEFTLA 664
           ASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGLLL+PN HDHGS+   E  L+
Sbjct: 605 ASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGLLLQPNAHDHGSEATQELMLS 664

Query: 665 VSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNET 724
            +K+HIQ  MAANL+DY+LTN EGKEVKGSEV  +D + VAYAS PTET+NYVSAHDNET
Sbjct: 665 TAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPVAYASLPTETINYVSAHDNET 724

Query: 725 LFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDW 784
           LFD++SLKTP  I+V++RCR+NHLA+S+IALSQGIPFFH GDE+LRSKS+DRDSYNSGDW
Sbjct: 725 LFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHAGDEILRSKSLDRDSYNSGDW 784

Query: 785 FNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIR 844
           FNRLDF+Y +NNWGVGLPPK KNE+NWPLIKPRL DPSFKP  SHI+A + NF +LL+IR
Sbjct: 785 FNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFKPKSSHIVATLHNFLDLLRIR 844

Query: 845 YSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVV 904
           YSSPLFRL TA AIQ+RVRFHNTG S IPG IVMSIEDG  GIP ++Q+DPIYS IVV+ 
Sbjct: 845 YSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGHRGIPSVSQIDPIYSLIVVIF 904

Query: 905 NARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFVELR 964
           NARPSE S+P PAL+ R L+LHP+Q+MS D +VK S Y+  +G F VP+RTT+VFVE R
Sbjct: 905 NARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDSFSGGFTVPARTTTVFVESR 963

BLAST of Cp4.1LG09g04070 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 125.6 bits (314), Expect = 2.2e-28
Identity = 137/542 (25.28%), Postives = 204/542 (37.64%), Query Frame = 0

Query: 289 DPYSRGVS-----SDGRRTLFVDLGSDDLKPEGWDKLADEKPPLHSFSDISIYELHVRDF 348
           DPY++ V       D  +      G+ D +   +D   D K P     D+ IYE++VR F
Sbjct: 187 DPYAKLVKGHSSFGDSSQKFAQFYGTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAF 246

Query: 349 SISDQS-VHPDLRGGYMGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVDDDKSKWK 408
           +  + S + P + G Y+GF     + I HL+ L   GI+ V LLP F+F           
Sbjct: 247 TADESSGMDPAIGGSYLGFI----EKIPHLQDL---GINAVELLPVFEFD---------- 306

Query: 409 SLDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLI-EF 468
             ++EL  +  P            ++     WGY+ + +  P   YAS    P +   EF
Sbjct: 307 --ELELQRRSNP------------RDHMVNTWGYSTVNFFAPMSRYASGEGDPIKASKEF 366

Query: 469 RKMVQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPG--YYLRRNIDGFIENSTCV 528
           ++MV+AL+  G+ V+LDVVYNH +      P +   + +    YY+    +  +  S C 
Sbjct: 367 KEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQLLNFSGCG 426

Query: 529 NNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGV 588
           N     H +V  +I+D + HWV +Y VDGFRFDL   L  +T     +A  ++       
Sbjct: 427 NTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRATDGSPLSAPPLIRAIAKDS 486

Query: 589 DGSSIYLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQG 648
             S   +  E WD G +   G+  N   ++ +    G + D VR  + G S         
Sbjct: 487 VLSRCKIIAEPWDCGGLYLVGKFPN---WDRWAEWNGMYRDDVRRFIKGDS--------- 546

Query: 649 FVTGLLLEPNDHDHGSDKEVEFTLAVSKDHIQVAMAANLRDYVLTNFEGKEVKGSEVRTY 708
                                                              +KGS     
Sbjct: 547 --------------------------------------------------GMKGSFATRV 606

Query: 709 DGSSVAY---ASCPTETVNYVSAHDNETLFDVVSLKTPRNIT----VNDRCRMNH----- 768
            GSS  Y      P   VN+V AHD  TL D+VS     N       ND C  NH     
Sbjct: 607 SGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCG 635

Query: 769 ------------LATS-------IIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRL 791
                       L T         + +SQG P    GDE   ++  + +SY      N  
Sbjct: 667 FEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNF 635

BLAST of Cp4.1LG09g04070 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 99.8 bits (247), Expect = 1.3e-20
Identity = 135/537 (25.14%), Postives = 210/537 (39.11%), Query Frame = 0

Query: 289 DPYSRGVSSDGRRTLFVDLGSDDLKPEGWDKLA----------DEKPPLH---SFSDISI 348
           DPY++ + S   R  F  LG DD     W ++A          D +  +H      D+ I
Sbjct: 167 DPYAKAIIS---RDEFGVLGPDD---NCWPQMACMVPTREEEFDWEGDMHLKLPQKDLVI 226

Query: 349 YELHVRDFSISDQSVHPDLRGGYMGFTLQDSDGITHLKKLSNAGISHVHLLPTFQFGGVD 408
           YE+HVR F+   +S   +  G Y G     ++ + HLK+L   GI+ + L+P  +F    
Sbjct: 227 YEMHVRGFT-RHESSKIEFPGTYQGV----AEKLDHLKEL---GINCIELMPCHEF---- 286

Query: 409 DDKSKWKSLDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSY--ASDPN 468
                              +  E  S    + +     WGY+ I +  P   Y  AS  N
Sbjct: 287 -------------------NELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNN 346

Query: 469 GPCRLI-EFRKMVQALNQIGLRVVLDVVYNHL---HGCGPFDPSSVLDKIVPGYYLRRNI 528
              R I EF+ +V+  ++ G+ V++DVV NH    +  GP      +D  V  YY+    
Sbjct: 347 FAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSV--YYMLAPK 406

Query: 529 DGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM--TSTMLKAKN 588
             F   S C N     H +V + I+D + +WV +  VDGFRFDL G +M  +S++  A N
Sbjct: 407 GEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVDGFRFDL-GSIMSRSSSLWDAAN 466

Query: 589 AL------------------QILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFN 648
                                ++    N      + L  E WD G + + G       + 
Sbjct: 467 VYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGGLYQVGM---FPHWG 526

Query: 649 LFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGSDKEVEFTLAVSKDH 708
           ++    G F D VR  + G   F       F   L   PN +  G           + D 
Sbjct: 527 IWSEWNGKFRDVVRQFIKGTDGF----SGAFAECLCGSPNLYQGGRKPWHSINFICAHDG 586

Query: 709 IQVAMAANLRDYVLTNFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFDVV 768
             +A      D V  N +     G E    DG          E  NY      E  F  +
Sbjct: 587 FTLA------DLVTYNNKNNLANGEE--NNDG----------ENHNYSWNCGEEGDFASI 631

Query: 769 SLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFN 787
           S+K  R      + +M +   S++ +SQG+P  + GDE   +K  + ++Y   ++ N
Sbjct: 647 SVKRLR------KRQMRNFFVSLM-VSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMN 631