Cp4.1LG09g03490 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g03490
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectinesterase
Location	Cp4.1LG09: 2104944 .. 2109803 (+)
RNA-Seq Expression	Cp4.1LG09g03490
Synteny	Cp4.1LG09g03490

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CACCGAGTCACTCCCGACTTGAATAAATTATGAACTCAGTTCTCATTTTCCTTCTCCTTCTCTCTCTCCGCCGCGCCGGAGCTTTCGATTCTCCTCCACCACCACCACCACCATCCGCCGACCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCCATCCGATTCTCTCCCTCCGATCCCTACGGCTACGGGAAATTCTCCGTCAAGAACTGCCTCAAACAGGCGATCAAAATGTCGAGCGTAATCGGCGACTATCTCGATCGAGGAAGCGATTCGAGGCTGAATCAGCGGGAGGCCGGCGCTCTGGAAGATTGCAGAGATCTTTCCGATTCAAACGTGGAGTTTCTCCGGTCGATTGAGAGCGTATTGGAGGCGGCGAAGAGCGTGGATGAGGAACTAGTGGAGCGCGTTCAGAGTATTTTGAGCGCGATCGTCACGAACGGCCAGACTTGTATAGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTAACGGCGCCGATGTCGAGCGCCGGGCAACTGTACAGTGTGTCGTTGGGATTGGTTACGAACTCGTTGTCGCGACTGTGGAAGAAGCGGCGGATGAAGGGAGACGGAGGGGATTCCGCGGCGGGCGCGCGGAACAGAGAGCCTCTGAACACGCTGATCAAGGTACGAATTACGCTGTTTGTCTGTTCGTTCAGTTACACGGAACTTTTCATTTGCTTTATTCAATGCATAAAACAAAAATTGGGGAAAATATATATATATATATTGGTTTAAAAAAAATAAATTAAAATGTAGCCCCTACCATTTTCAAAATTACAGTTTTGTCCCCTATGATAAAATTTTATAAATAGTTTGAAAACTATATTTATGAAATTTTCTAATTTTTAAAATTATATAGACTAAATTTTAATTTTTTCTATATTATAGGGATGAAATTTGTAATTTATGGTTATTTTTTTAAAAATATTACCATATCTATAAGAGGATAATTTGGATCAAGAAAGGGTAAAAATGTAATTACACTCGTGGATAAATATGATTTAAGAAGAAGAAAAAAGAAAATATTAAATAGAAAAAACATTTAAAAATATCTAATAAAATTATAAAATTTTAATTTTGATCTATTTTGTGTTAAAATAAGTTTTTTTTTTATTATTTTTTTTTAATTTCGTGTTTAATTGACATTTAAACTTTTAATAATTAGTAAAATAAAATTTAATTACAGAATTAAAAATTCTTAAAAATTTATTAAATATATATTTGTAAAAAGGGTATGAAATTCACCTATTATTACACACATTCAATTTCTTTGATTATGCACTATGTTTAAAAGAAAATTAGTTGAATTATTATTTTGACTTTCAAATTTAATTCTATTTTAACATTTTTTTAATATTCAAATTTAGTTCAAGATCTTAAAATTAGTTCATTATGTTAAATTATTAATAAATTTTCTAAAAAAATATTATAGGTTATTCTTATATTTATAAATTTTAGAAATATATTTATCTTCGTAAAATAATAATATTATTTATCTATATTCTGTAAGTACATACTTCATTTTTTTTTTTTTAAATGTATTGCTTTAAAAGTGGATCCAACTCTCCCATGACCTTATTATTCTCCCCCACAAAGGGATATTAATGGGGCAAAAACCAGTTGTTTATATTAAAATATTAATTGTATTAAATATCCTTCAACCATATGAAATTAAATTGTGGGTAGATATTATTTAGAGGAGTTAGGAGTTGGGAAGTGGTACATAATTATATAATGTAAAAACTATACATTAATTTTATTTAATAACTTTATCCTGTTTTGGCTTCTTGGCGGAGCTGTAGACTTATCTCTCTTAATACTGTTAATTAACGTGTGAAATTAGGGGGAAAAAACAGAGGCATGTGCAAATATTAATGGAATTTAATATCCACCAACCAAAATAATTTAAATATGGAGGAAAATTATCACAAGAGGGGTGGAATATAATAATAAATTATGCACAAATTTAGCTTAATCTGTATTTAATTAATTGCTGATCTCTCTTAATTAGAATATTAGTATGAATATATTTGTTCTCTTTCTTGGATGATGAAAGTGATGAAAGTACCACGTCAGTTCATTTAGGGAATGATCGTGGGTTTATAAACAAAGAATACTCTCTCCATTGGTATGAGGCCTTTTGGGGAAGTCCAAAATAAAGCCACGAGAGCTTATACTAGTGGACAATATCAAAAAAAAAGAAAAAGTACGGAGGTGTTAGATGATGAAAGTCCCACGTCGGCTAATTTAGGGAATGAGGCTTTTGGAGAAGTCCAAAGCAAAGTCATGAATACCATTATGGAGAGTCATGTTCTTTTCATTTATTTATGTTTTCTCGGTGTCACGAGAGAAATTTAACTTAGTTTAATATTGTTTTATTCTTATCTAGACATTTGGTTCTTGAAATTTAAAATGGACATATTTGATGGAGATATTTGAAGTAATCGATATAATAGAACGATGACATAACCGATCGAATAGGGTGACTAGAAATTGCTTGCATTTATTAAATTTGTTGGCTGATTCTTTAGGAAACTATAGGCTTAATGGGTTAAGTTTGCATCAGGGACTACATAAAATGGGTTCATGCAACCAATCCACGGGCTGCTCAGGAAGGAAGCGTTTGTTGGCTGATTTAGGCACCACAGGAATCTTCATCAACGATACTGTGGTTGTTAGCTCTACTGGTGCAGACAACTTCACCTCAATTGGAGATGCTATTGCCTTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGGAGGATACTATGAAGAATACGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCGTTGGTGATGGCATTAACCGAACCGTCATCACTGGGAACCATAGCGTTGTCGATGGTTGGACGACGTATAATTCCTCTACATTTAGTAAGTAAATCGTAAACTCATAACCTGTTTACTTATTTTCGCTGAATTCTAAGTAGAGATCTTCATGAGGTAGGATGAAGACGGGGAAACTGTGAGATCCCATATTAGTTGGAGAGGAGACTGAAACATTCTTTATTAAGTGTGTGGAATCCTCTCTCTCTAACAGATGCGTTTTAAAAATCTCAAGGGGAAGTCTCAAAGAGGACCATATTTGTGAGTGGTGGGCTTGAGCTGTTACTAATGGTATTAGAGCTAAACACGGGGTGATGTGTTAGCAAGGAGGTTGAGCCCCAAAGGGGGGTGGACACGAGGTGGTGTGCCAAGAAGGACATTGGGCCCCGAAGGGTGGTGGATTGGAGGGTCCCACATCGATTGGAGAGGGAAATGAATGCCAGCGAGGACGCTGGGGATCGAAAGGGGGTGGATTGTGAGATCCCACATCAGTTGGGGAGGAGAACGAAACATTCCTTATAAGAGTGTGGAAACCTCTCCCTAGGAGACGTGTTTTAAAAACCTTGAGGGGAAGCTCGAAAGGGAAAGCCTAAAGAAGACCATATTTGCTAGCACTGGGTTTGGGTTGTTATTAATGGTATCAGAGCCAGACACTGGGCGATGCGTTAGCGAGGAGGCTAAGCCCCGAAGGGGGGTGGACACGAGGCGGTGTACCAGCAAGGACGCTGGACCCCGAAGGGTGGTAGACACGAGACAGTGTACCAACAAGGACACTAGACCTCGAAGGGTGGTGGAGTGGGTAGTCCCATGTCGATTGGACAAATGAATGTCAGCTAGGACGCTGAGCCCTGAAGGGGGGGGGGGGGGGNGGAGGAGAACGAAGCATTCTTTATAAGAGCGTGGAAACCTCTCCGTAGCAGACGCGTTTTACAAACTTTGAGGGAAAGTCCGAAAGAGAATGCCCAAAGAGGACAATATTTGCTAGCGATGAACTTGAGTTCTTACATAAGCCTGCTTTGTCTCTGTCCTCACTTCCTATTCATACATCATCCTCACGATGGCGGTCCAATCTAGTTGACAGGGACGCCCAGCCTCGTTTAACTAATAGGAAGAAATCTCTCACCACTCCCTCTCCATTCTTCGACCCGTTCAGTGCAGTTCCCGTGTATCATGTTAGTTTTACGCTGTTTGATATATAAATCTTAGTTTAATGCTTGGATTTTATGTGCTATATGTTTGCAGCTGTTTGTGGGGATAGATTTGTAGCAATAGATGTGACATTTAGGAACACAGCAGGGCCAGAGAAGCACCAAGCAGTAGCACTAAGGAGCAGTGCAGATCTCTCAACATTCTACCGCTGCAGCTTCGAGGGCTATCAAGACACTCTCTACGTCCATTCCCTAAGACAATTCTACCGAGAATGCGACATTTACGGCACAGTAGATTTCATATTTGGCAATGCCGCTGCAGTTTTCCAAAAATGCAATCTATACGCCAGAAAGCCACTCCCGAACCAAAAGATCGCCATCACGGCACAGGGCCGAACCGATCCGAACCAAAACACAGGTATCTCGATCCACGACTGCACGATCAAGGCAGCCCCCGACTGGGAAACGGATTACTCAAACTCGACGACGACAAACTATCTTGGACGGCCGTGGAAGCAGTACTCAAGAACAGTGTATATGCAGTGTTACATTGGGGATTTGATCAACCCTGTGGGGTGGTTGGAGTGGAATGGAACAGCAGGGTTGGACACTTTGTATTATGGGGAGTTTGAGAATTATGGGCCAGGAGCTAACACAAGCTTGAGGGTGGATTGGCCTGGCTATAGCTTGATGAATATTACTCAAGCTATGAACTTTACTGTCTATAATCTTACTATGGGAGATACATGGCTGCCTTACACTGATATTCCCTTCTCTGGTGGTTTGGTC

mRNA sequence

CACCGAGTCACTCCCGACTTGAATAAATTATGAACTCAGTTCTCATTTTCCTTCTCCTTCTCTCTCTCCGCCGCGCCGGAGCTTTCGATTCTCCTCCACCACCACCACCACCATCCGCCGACCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCCATCCGATTCTCTCCCTCCGATCCCTACGGCTACGGGAAATTCTCCGTCAAGAACTGCCTCAAACAGGCGATCAAAATGTCGAGCGTAATCGGCGACTATCTCGATCGAGGAAGCGATTCGAGGCTGAATCAGCGGGAGGCCGGCGCTCTGGAAGATTGCAGAGATCTTTCCGATTCAAACGTGGAGTTTCTCCGGTCGATTGAGAGCGTATTGGAGGCGGCGAAGAGCGTGGATGAGGAACTAGTGGAGCGCGTTCAGAGTATTTTGAGCGCGATCGTCACGAACGGCCAGACTTGTATAGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTAACGGCGCCGATGTCGAGCGCCGGGCAACTGTACAGTGTGTCGTTGGGATTGGTTACGAACTCGTTGTCGCGACTGTGGAAGAAGCGGCGGATGAAGGGAGACGGAGGGGATTCCGCGGCGGGCGCGCGGAACAGAGAGCCTCTGAACACGCTGATCAAGGGACTACATAAAATGGGTTCATGCAACCAATCCACGGGCTGCTCAGGAAGGAAGCGTTTGTTGGCTGATTTAGGCACCACAGGAATCTTCATCAACGATACTGTGGTTGTTAGCTCTACTGGTGCAGACAACTTCACCTCAATTGGAGATGCTATTGCCTTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGGAGGATACTATGAAGAATACGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCGTTGGTGATGGCATTAACCGAACCGTCATCACTGGGAACCATAGCGTTGTCGATGGTTGGACGACGTATAATTCCTCTACATTTAGAGCTAACACAAGCTTGAGGGTGGATTGGCCTGGCTATAGCTTGATGAATATTACTCAAGCTATGAACTTTACTGTCTATAATCTTACTATGGGAGATACATGGCTGCCTTACACTGATATTCCCTTCTCTGGTGGTTTGGTC

Coding sequence (CDS)

ATGAACTCAGTTCTCATTTTCCTTCTCCTTCTCTCTCTCCGCCGCGCCGGAGCTTTCGATTCTCCTCCACCACCACCACCACCATCCGCCGACCTCTGTAAATCCACTCTCTACCCTAAGCTCTGCCGCTCCATTCTCTCCTCCATCCGATTCTCTCCCTCCGATCCCTACGGCTACGGGAAATTCTCCGTCAAGAACTGCCTCAAACAGGCGATCAAAATGTCGAGCGTAATCGGCGACTATCTCGATCGAGGAAGCGATTCGAGGCTGAATCAGCGGGAGGCCGGCGCTCTGGAAGATTGCAGAGATCTTTCCGATTCAAACGTGGAGTTTCTCCGGTCGATTGAGAGCGTATTGGAGGCGGCGAAGAGCGTGGATGAGGAACTAGTGGAGCGCGTTCAGAGTATTTTGAGCGCGATCGTCACGAACGGCCAGACTTGTATAGACGGATTGGTGGAATCGAGGAGTAGTTTGGGGAATGCGCTAACGGCGCCGATGTCGAGCGCCGGGCAACTGTACAGTGTGTCGTTGGGATTGGTTACGAACTCGTTGTCGCGACTGTGGAAGAAGCGGCGGATGAAGGGAGACGGAGGGGATTCCGCGGCGGGCGCGCGGAACAGAGAGCCTCTGAACACGCTGATCAAGGGACTACATAAAATGGGTTCATGCAACCAATCCACGGGCTGCTCAGGAAGGAAGCGTTTGTTGGCTGATTTAGGCACCACAGGAATCTTCATCAACGATACTGTGGTTGTTAGCTCTACTGGTGCAGACAACTTCACCTCAATTGGAGATGCTATTGCCTTTGCTCCCAACAATTCAATGCCTCAAGATGGCTACTTTGTTATCTATGTTAGAGGAGGATACTATGAAGAATACGTCGTCGTCCCCAAATACAAAACCAACATAATGCTCGTTGGTGATGGCATTAACCGAACCGTCATCACTGGGAACCATAGCGTTGTCGATGGTTGGACGACGTATAATTCCTCTACATTTAGAGCTAACACAAGCTTGAGGGTGGATTGGCCTGGCTATAGCTTGATGAATATTACTCAAGCTATGAACTTTACTGTCTATAATCTTACTATGGGAGATACATGGCTGCCTTACACTGATATTCCCTTCTCTGGTGGTTTGGTC

Protein sequence

MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFRANTSLRVDWPGYSLMNITQAMNFTVYNLTMGDTWLPYTDIPFSGGLV

Homology

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BLAST of Cp4.1LG09g03490 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 8.2e-80
Identity = 197/555 (35.50%), Postives = 256/555 (46.13%), Query Frame = 0


Query: 21  SPPPPPPPSAD-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMS 80
           S PP  PPS        CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA ++S
Sbjct: 66  SQPPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLS 125

Query: 81  SVIGDYLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERV 140
            VI  Y  R           E GA+ DC +LS+ +V +L ++ + L+ A+ +   LVE V
Sbjct: 126 KVITSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHV 185

Query: 141 QSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRM 200
            S+LS +VTN QTC+DGLVE++S    A+ +PM +  +LYS+SLGLV+++L+R    +R 
Sbjct: 186 NSLLSGVVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNR--NLKRF 245

Query: 201 KGDGGDSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFIND 260
           K   G    G  +  REPL TLIKGL K  +C+    C    R L +LG T    I ++ 
Sbjct: 246 KASKGKILGGGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSK 305

Query: 261 TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGD 320
            V+V    +DNFT+I DAIA APNN+ P+DGYFVIY R G YEEY+VVP  K N+ML+GD
Sbjct: 306 AVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGD 365

Query: 321 GINRTVITGNHSVVDGWTTYNSSTFR---------------------------------- 380
           GIN+T+ITGNH+VVDGWTTYN S+F                                   
Sbjct: 366 GINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGS 425

Query: 381 ------------------------------------------------------------ 382
                                                                       
Sbjct: 426 SFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNA 485

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BLAST of Cp4.1LG09g03490 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 4.2e-76
Identity = 190/550 (34.55%), Postives = 250/550 (45.45%), Query Frame = 0


Query: 21  SPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGD 80
           S P P PP+   CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA ++S VI  
Sbjct: 77  SQPSPLPPNI-ACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINR 136

Query: 81  YLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILS 140
           +  R           E  A+ DC +L++ +VE+L ++   L+AA+ +   LV+RV S+L 
Sbjct: 137 FAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLG 196

Query: 141 AIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGDGG 200
            +VTN QTC+DGLV+++S    A+  P+ +  +LYSVSLGLV+++L+R    +R KG  G
Sbjct: 197 GVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNR--NLKRYKGSKG 256

Query: 201 DSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFINDTVVVS 260
               G     REPL TLIK L K  +C++   C    R L +LG T    I + + V V 
Sbjct: 257 KIFGGGNKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVG 316

Query: 261 STGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGDGINRT 320
               DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIML+GDGIN+T
Sbjct: 317 PYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKT 376

Query: 321 VITGNHSVVDGWTTYNSSTFR--------------------------------------- 380
           +I+GNHS +DGWTTYNSSTF                                        
Sbjct: 377 IISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRC 436

Query: 381 ------------------------------------------------------------ 382
                                                                       
Sbjct: 437 SFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHG 496

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BLAST of Cp4.1LG09g03490 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 184.1 bits (466), Expect = 2.9e-45
Identity = 133/368 (36.14%), Postives = 201/368 (54.62%), Query Frame = 0


Query: 1   MNSVLIFL--LLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           M S+ +FL  L LSL+          P   S+ +CK+T  PK C+S+    + S  D   
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVRE 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFL-RSIES 120
           YG+FS++  L Q+ K +  I  YL R +++ L+Q   GAL+DCR L+    ++L  S E+
Sbjct: 61  YGRFSLRKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFET 120

Query: 121 V--LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSS---LGNALTAPMSSAGQL 180
           V    ++K++     + +Q++LSA +TN QTC+DG+  + SS   + N +  P+ +  +L
Sbjct: 121 VNITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKL 180

Query: 181 YSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARN----REPLNTLIKGL--HKMGSCNQS 240
           +SVSL L T      W  ++ K     S A  +N     +P      G    KM    ++
Sbjct: 181 FSVSLALFTKG----WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRA 240

Query: 241 TGCSGRKRLLA--DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 300
              S  +R LA  D     + ++D V V+  G  NFT+I +A+  APN +    GYFVIY
Sbjct: 241 VYESLSRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 300

Query: 301 VRGGYYEEYVVVPKYKTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFRANTSLRVDWP 353
           V  G YEE VV+ K K  +M++GDGINRTV+TGN +VVDGWTT+NS+TF   +      P
Sbjct: 301 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTS------P 355

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BLAST of Cp4.1LG09g03490 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 175.6 bits (444), Expect = 1.0e-42
Identity = 117/312 (37.50%), Postives = 168/312 (53.85%), Query Frame = 0


Query: 30  ADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDSR 89
           +++C+    P  CRS+L +    P D Y YG+ S++  L +A +  S+I   LDR     
Sbjct: 36  SNICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRFISMIDAELDR-KGKV 95

Query: 90  LNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILSAIVTNGQTCID 149
             +   GALEDC+ L+   +++L S     ++ K++     E V + LSA +TN QTC++
Sbjct: 96  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLE 155

Query: 150 GLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG-------DGGDSAA 209
           GL  + S   N L+  + +  +LY VSL L     S+ W  RR +               
Sbjct: 156 GLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQRSRPIWQPQARFKKFF 215

Query: 210 GARN-REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINDTVVVSSTGADNFT 269
           G RN + PL        KM    ++   +  +R L       + ++D V V   G  NFT
Sbjct: 216 GFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFT 275

Query: 270 SIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGDGINRTVITGNHSV 329
           +I  AIA APN +   +GYF+IYV  G YEEYV VPK K  +M++GDGIN+TVITGN SV
Sbjct: 276 TINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSV 329

Query: 330 VDGWTTYNSSTF 334
           VDGWTT+NS+TF
Sbjct: 336 VDGWTTFNSATF 329

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BLAST of Cp4.1LG09g03490 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 3.1e-39
Identity = 119/345 (34.49%), Postives = 182/345 (52.75%), Query Frame = 0


Query: 29  SADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDS 88
           S+ +CK+T  PK C+S+      S  +   YG FS++  L Q+ K    +  Y+ R  ++
Sbjct: 28  SSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSIRKSLSQSRKFIRTVDRYIKR--NA 87

Query: 89  RLNQREA-GALEDCRDLSDSNVEF-LRSIESVLEAA-----KSVDEELVERVQSILSAIV 148
            L+Q     AL+DCR L+   +++ L S E+V + +     K +     + +Q++LSA +
Sbjct: 88  HLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSFPKADDIQTLLSAAL 147

Query: 149 TNGQTCIDGLVESRS-----SLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGD 208
           TN QTC++GL  + S     ++   +  P+ +  +L  VSL L T       KKR     
Sbjct: 148 TNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTKGWVPKKKKRAGFAW 207

Query: 209 GGDSAAGARNREPLNTLIKGL--HKMGSCNQSTGCSGRKRLLADLGTTG-------IFIN 268
               +  + + +P      G    KM    ++   S  +R LAD  + G       + I+
Sbjct: 208 AQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGDSNGDGDDGSMVLIS 267

Query: 269 DTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVG 328
           D V VS  G  NFT+I  A+A APNN+    G+F+IYV  G YEEY+ + K K  +M++G
Sbjct: 268 DIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIG 327

Query: 329 DGINRTVITGNHSVVDGWTTYNSSTFRANTSLRVDWPGYSLMNIT 353
           DGIN+TV+TGN SVVDGWTT+NS+TF       V  P +  +NIT
Sbjct: 328 DGINQTVVTGNRSVVDGWTTFNSATF------AVTAPNFVAVNIT 361

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BLAST of Cp4.1LG09g03490 vs. NCBI nr
Match: XP_023541032.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 676 bits (1745), Expect = 6.42e-241
Identity = 380/565 (67.26%), Postives = 380/565 (67.26%), Query Frame = 0


Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300

Query: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR-------------------------- 360
           TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTF                           
Sbjct: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

[Close]

BLAST of Cp4.1LG09g03490 vs. NCBI nr
Match: XP_022945508.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata] >XP_022945509.1 probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata])

HSP 1 Score: 673 bits (1737), Expect = 1.06e-239
Identity = 377/565 (66.73%), Postives = 379/565 (67.08%), Query Frame = 0


Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYV+VPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKYK 300

Query: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR-------------------------- 360
           TNIML+GDGINRTVITGNHSVVDGWTTYNSSTF                           
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

[Close]

BLAST of Cp4.1LG09g03490 vs. NCBI nr
Match: KAG6573907.1 (putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667 bits (1721), Expect = 2.97e-237
Identity = 377/566 (66.61%), Postives = 379/566 (66.96%), Query Frame = 0


Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPP-SADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPP SADLCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL
Sbjct: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL
Sbjct: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           VTNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL
Sbjct: 181 VTNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240

Query: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY
Sbjct: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300

Query: 301 KTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR------------------------- 360
           KTNIML+GDGINRTVITGNHSVVDGWTTYNSSTF                          
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 ALRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYAR 420

[Close]

BLAST of Cp4.1LG09g03490 vs. NCBI nr
Match: XP_022968171.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 665 bits (1716), Expect = 1.59e-236
Identity = 375/565 (66.37%), Postives = 377/565 (66.73%), Query Frame = 0


Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPP SADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPP-SADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300

Query: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR-------------------------- 360
           TNIML+GDGINRTVITGNHSVVDGWTTYNSSTF                           
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

[Close]

BLAST of Cp4.1LG09g03490 vs. NCBI nr
Match: XP_022976564.1 (probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima])

HSP 1 Score: 561 bits (1446), Expect = 1.62e-195
Identity = 324/567 (57.14%), Postives = 350/567 (61.73%), Query Frame = 0


Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNS   IFL LLSLR AGA DSPPP    SA LCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPP----SAVLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSV+ CLKQAIKMSSVIG+YLDRG DSRLN++E GALEDCR+LSDSNVEFL+SI+ VL
Sbjct: 61  GKFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAA+ VDEEL+ER++SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+ SAG+LYSVSLGL
Sbjct: 121 EAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGD--GGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLA 240
           V NSLSR WKKR  KGD  GG+  A A++REPLNTLIKGLH MGSCNQST CSGRKR+L 
Sbjct: 181 VKNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLT 240

Query: 241 DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVP 300
           DLGTTGI IN+TVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR GYY+EYVVVP
Sbjct: 241 DLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVP 300

Query: 301 KYKTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR----------------------- 360
           KYKTNIML+GDGINRTVITGNH+VVDGWTTYNSSTF                        
Sbjct: 301 KYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQ 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 AVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLY 420

[Close]

BLAST of Cp4.1LG09g03490 vs. ExPASy TrEMBL
Match: A0A6J1G137 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449722 PE=3 SV=1)

HSP 1 Score: 673 bits (1737), Expect = 5.12e-240
Identity = 377/565 (66.73%), Postives = 379/565 (67.08%), Query Frame = 0


Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGD AAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDIAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYV+VPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVIVPKYK 300

Query: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR-------------------------- 360
           TNIML+GDGINRTVITGNHSVVDGWTTYNSSTF                           
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

[Close]

BLAST of Cp4.1LG09g03490 vs. ExPASy TrEMBL
Match: A0A6J1HU46 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1)

HSP 1 Score: 665 bits (1716), Expect = 7.72e-237
Identity = 375/565 (66.37%), Postives = 377/565 (66.73%), Query Frame = 0


Query: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60
           MNSVLIFLLLLSLRRAGAFDSPPPPPP SADLCKSTLYPKLCRSILSSIRFSPSDPYGYG
Sbjct: 1   MNSVLIFLLLLSLRRAGAFDSPPPPPP-SADLCKSTLYPKLCRSILSSIRFSPSDPYGYG 60

Query: 61  KFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120
           KFSVKNCLKQAIKMSSVIGDYLDRG DSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE
Sbjct: 61  KFSVKNCLKQAIKMSSVIGDYLDRGGDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLE 120

Query: 121 AAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180
           AAK VDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV
Sbjct: 121 AAKGVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLV 180

Query: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240
           TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG
Sbjct: 181 TNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLG 240

Query: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300
           TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK
Sbjct: 241 TTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYK 300

Query: 301 TNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR-------------------------- 360
           TNIML+GDGINRTVITGNHSVVDGWTTYNSSTF                           
Sbjct: 301 TNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDRFVAIDVTFRNTAGPEKHQAVA 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 LRSSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAVFQKCNLYARK 420

[Close]

BLAST of Cp4.1LG09g03490 vs. ExPASy TrEMBL
Match: A0A6J1INW0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1)

HSP 1 Score: 561 bits (1446), Expect = 7.84e-196
Identity = 324/567 (57.14%), Postives = 350/567 (61.73%), Query Frame = 0


Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNS   IFL LLSLR AGA DSPPP    SA LCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSAPFIFLFLLSLRLAGASDSPPP----SAVLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSV+ CLKQAIKMSSVIG+YLDRG DSRLN++E GALEDCR+LSDSNVEFL+SI+ VL
Sbjct: 61  GKFSVRKCLKQAIKMSSVIGEYLDRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAA+ VDEEL+ER++SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+ SAG+LYSVSLGL
Sbjct: 121 EAAEGVDEELMERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGD--GGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLA 240
           V NSLSR WKKR  KGD  GG+  A A++REPLNTLIKGLH MGSCNQST CSGRKR+L 
Sbjct: 181 VKNSLSRRWKKRSQKGDREGGNFPAAAQSREPLNTLIKGLHSMGSCNQSTKCSGRKRMLT 240

Query: 241 DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVP 300
           DLGTTGI IN+TVVVSSTGADNFTSIGDAI+FAPNNSMPQDGYFVIYVR GYY+EYVVVP
Sbjct: 241 DLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMPQDGYFVIYVRQGYYDEYVVVP 300

Query: 301 KYKTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR----------------------- 360
           KYKTNIML+GDGINRTVITGNH+VVDGWTTYNSSTF                        
Sbjct: 301 KYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQ 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 AVAVRNSADLSTFYRCSFEGYQDTLYVHSLRQFYRECDVYGTVDFIFGNAAAVFQQCNLY 420

[Close]

BLAST of Cp4.1LG09g03490 vs. ExPASy TrEMBL
Match: A0A6J1D8L4 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1)

HSP 1 Score: 547 bits (1410), Expect = 1.79e-190
Identity = 321/565 (56.81%), Postives = 344/565 (60.88%), Query Frame = 0


Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNS+  IFLLLLSLR AGA DSPPP    SA LCK+TLYPKLC SILSSIRFSPSDPYGY
Sbjct: 1   MNSLPYIFLLLLSLRLAGASDSPPP----SAALCKTTLYPKLCHSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVK CLKQAIKMSSVIG YL RG  SRLNQREAGAL+DCR+L+D NVEFLRSIESVL
Sbjct: 61  GKFSVKQCLKQAIKMSSVIGGYLRRGGGSRLNQREAGALDDCRELADLNVEFLRSIESVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAA+ VDE++VERV+SILSAIVTNGQTCIDGLVES+SSLG+AL+AP+S+AG+LYSVSLGL
Sbjct: 121 EAAEGVDEQVVERVESILSAIVTNGQTCIDGLVESKSSLGSALSAPLSTAGELYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDGGDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLADL 240
           V NSLSR WKKRR    GG       NREPL TLIKGL KMGSCNQST CSGR+RLL DL
Sbjct: 181 VKNSLSRRWKKRRQSFPGGGG-----NREPLITLIKGLRKMGSCNQSTICSGRERLLTDL 240

Query: 241 GTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKY 300
           GTTGIFIN+TVVVSSTGADNFTSIGDAIAF+PNNSMP+DGYFVIYVR GYYEEYVVVPKY
Sbjct: 241 GTTGIFINNTVVVSSTGADNFTSIGDAIAFSPNNSMPEDGYFVIYVREGYYEEYVVVPKY 300

Query: 301 KTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR------------------------- 360
           KTNIML+GDGINRTVITGNHSVVDGWTTYNSSTF                          
Sbjct: 301 KTNIMLIGDGINRTVITGNHSVVDGWTTYNSSTFTVCGDGFVAIDVTFRNTAGPEKHQAV 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 AVRNSADLSTFYRCSFEGYQDTLYVHSFRQFYRECDIYGTVDFIFGNAAAVFQQCNLYAR 420

[Close]

BLAST of Cp4.1LG09g03490 vs. ExPASy TrEMBL
Match: A0A6J1GYC3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1)

HSP 1 Score: 541 bits (1393), Expect = 9.25e-188
Identity = 320/570 (56.14%), Postives = 346/570 (60.70%), Query Frame = 0


Query: 1   MNSV-LIFLLLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGY 60
           MNSV  IFLL LSL  AGA DS PP    SA LCKSTLYPKLCRSILSSIRFSPSDPYGY
Sbjct: 1   MNSVPFIFLLFLSLGLAGASDSAPP----SAFLCKSTLYPKLCRSILSSIRFSPSDPYGY 60

Query: 61  GKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVL 120
           GKFSVK CLKQAIKMSSVIG+YL+RG DSRLN++E GALEDCR+LSDSNVEFL+SI+ VL
Sbjct: 61  GKFSVKKCLKQAIKMSSVIGEYLNRGGDSRLNRQEVGALEDCRELSDSNVEFLQSIDGVL 120

Query: 121 EAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGL 180
           EAA+ VDEELVER++SILSAIVTNGQTCIDGLVESRSSLGNAL+AP+ SAG+LYSVSLGL
Sbjct: 121 EAAEGVDEELVERIESILSAIVTNGQTCIDGLVESRSSLGNALSAPLLSAGELYSVSLGL 180

Query: 181 VTNSLSRLWKKRRMKGDG--GDSAAGARNREPLNTLIKGLHKMGSCNQSTGCSGRKRLLA 240
           V NSLSR WKKRR KGDG  G+  A A++REPLNTLIKGLH M SCNQST C GRKRLL 
Sbjct: 181 VKNSLSRRWKKRRQKGDGEGGNFPAAAQSREPLNTLIKGLH-MSSCNQSTKCWGRKRLLT 240

Query: 241 DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVP 300
           DLGTTGI IN+TVVVSSTGADNFTSIGDAI+FAPNNSM QDGYFVIYVR GYY+EYV+VP
Sbjct: 241 DLGTTGILINNTVVVSSTGADNFTSIGDAISFAPNNSMLQDGYFVIYVRQGYYDEYVIVP 300

Query: 301 KYKTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFR----------------------- 360
           KYKTNIML+GDGINRTVITGNH+VVDGWTTYNSSTF                        
Sbjct: 301 KYKTNIMLIGDGINRTVITGNHNVVDGWTTYNSSTFTVYGDGFVAIDMTFRNTAGAEKHQ 360

Query: 361 ------------------------------------------------------------ 381
                                                                       
Sbjct: 361 AVAVRNSADLSTFYRCSFEGYQDTLYVHSMRQFYRECDVYGTVDFIFGNAAAVFQQCNLY 420

[Close]

BLAST of Cp4.1LG09g03490 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 298.9 bits (764), Expect = 5.8e-81
Identity = 197/555 (35.50%), Postives = 256/555 (46.13%), Query Frame = 0


Query: 21  SPPPPPPPSAD-----LCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMS 80
           S PP  PPS        CKST YPKLCR+IL++++ SPSDPY YGKF++K CLKQA ++S
Sbjct: 66  SQPPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLS 125

Query: 81  SVIGDYLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERV 140
            VI  Y  R           E GA+ DC +LS+ +V +L ++ + L+ A+ +   LVE V
Sbjct: 126 KVITSYARRVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELKTAQVMTAALVEHV 185

Query: 141 QSILSAIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRM 200
            S+LS +VTN QTC+DGLVE++S    A+ +PM +  +LYS+SLGLV+++L+R    +R 
Sbjct: 186 NSLLSGVVTNQQTCLDGLVEAKSGFAAAIGSPMGNLTRLYSISLGLVSHALNR--NLKRF 245

Query: 201 KGDGGDSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFIND 260
           K   G    G  +  REPL TLIKGL K  +C+    C    R L +LG T    I ++ 
Sbjct: 246 KASKGKILGGGNSTYREPLETLIKGLRK--TCDNDKDCRKTSRNLGELGETSGGSILVSK 305

Query: 261 TVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGD 320
            V+V    +DNFT+I DAIA APNN+ P+DGYFVIY R G YEEY+VVP  K N+ML+GD
Sbjct: 306 AVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGD 365

Query: 321 GINRTVITGNHSVVDGWTTYNSSTFR---------------------------------- 380
           GIN+T+ITGNH+VVDGWTTYN S+F                                   
Sbjct: 366 GINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGS 425

Query: 381 ------------------------------------------------------------ 382
                                                                       
Sbjct: 426 SFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNA 485

[Close]

BLAST of Cp4.1LG09g03490 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 286.6 bits (732), Expect = 3.0e-77
Identity = 190/550 (34.55%), Postives = 250/550 (45.45%), Query Frame = 0


Query: 21  SPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGD 80
           S P P PP+   CKST YPKLCR+ILS+++ SPSDPY YGKF++K CLKQA ++S VI  
Sbjct: 77  SQPSPLPPNI-ACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINR 136

Query: 81  YLDR--GSDSRLNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILS 140
           +  R           E  A+ DC +L++ +VE+L ++   L+AA+ +   LV+RV S+L 
Sbjct: 137 FAQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAELMTAALVDRVTSLLG 196

Query: 141 AIVTNGQTCIDGLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGDGG 200
            +VTN QTC+DGLV+++S    A+  P+ +  +LYSVSLGLV+++L+R    +R KG  G
Sbjct: 197 GVVTNQQTCLDGLVDAKSGFATAIGTPLGNLTRLYSVSLGLVSHALNR--NLKRYKGSKG 256

Query: 201 DSAAGARN--REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTT---GIFINDTVVVS 260
               G     REPL TLIK L K  +C++   C    R L +LG T    I + + V V 
Sbjct: 257 KIFGGGNKPVREPLETLIKVLRK--TCDKGKDCRKANRNLGELGETSGGSILVREAVTVG 316

Query: 261 STGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGDGINRT 320
               DNF +I +A+A APN++ P+ GYFVIY R G YEEYVV+   K NIML+GDGIN+T
Sbjct: 317 PYETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKT 376

Query: 321 VITGNHSVVDGWTTYNSSTFR--------------------------------------- 380
           +I+GNHS +DGWTTYNSSTF                                        
Sbjct: 377 IISGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRC 436

Query: 381 ------------------------------------------------------------ 382
                                                                       
Sbjct: 437 SFEGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHG 496

[Close]

BLAST of Cp4.1LG09g03490 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 184.1 bits (466), Expect = 2.1e-46
Identity = 133/368 (36.14%), Postives = 201/368 (54.62%), Query Frame = 0


Query: 1   MNSVLIFL--LLLSLRRAGAFDSPPPPPPPSADLCKSTLYPKLCRSILSSIRFSPSDPYG 60
           M S+ +FL  L LSL+          P   S+ +CK+T  PK C+S+    + S  D   
Sbjct: 1   MLSLKLFLVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFP--QTSQGDVRE 60

Query: 61  YGKFSVKNCLKQAIKMSSVIGDYLDRGSDSRLNQREAGALEDCRDLSDSNVEFL-RSIES 120
           YG+FS++  L Q+ K +  I  YL R +++ L+Q   GAL+DCR L+    ++L  S E+
Sbjct: 61  YGRFSLRKSLTQSRKFTRTIDRYLKR-NNALLSQSAVGALQDCRYLASLTTDYLITSFET 120

Query: 121 V--LEAAKSVDEELVERVQSILSAIVTNGQTCIDGLVESRSS---LGNALTAPMSSAGQL 180
           V    ++K++     + +Q++LSA +TN QTC+DG+  + SS   + N +  P+ +  +L
Sbjct: 121 VNITTSSKTLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKL 180

Query: 181 YSVSLGLVTNSLSRLWKKRRMKGDGGDSAAGARN----REPLNTLIKGL--HKMGSCNQS 240
           +SVSL L T      W  ++ K     S A  +N     +P      G    KM    ++
Sbjct: 181 FSVSLALFTKG----WVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRA 240

Query: 241 TGCSGRKRLLA--DLGTTGIFINDTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIY 300
              S  +R LA  D     + ++D V V+  G  NFT+I +A+  APN +    GYFVIY
Sbjct: 241 VYESLSRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIY 300

Query: 301 VRGGYYEEYVVVPKYKTNIMLVGDGINRTVITGNHSVVDGWTTYNSSTFRANTSLRVDWP 353
           V  G YEE VV+ K K  +M++GDGINRTV+TGN +VVDGWTT+NS+TF   +      P
Sbjct: 301 VTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTS------P 355

[Close]

BLAST of Cp4.1LG09g03490 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 175.6 bits (444), Expect = 7.4e-44
Identity = 117/312 (37.50%), Postives = 168/312 (53.85%), Query Frame = 0


Query: 30  ADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDSR 89
           +++C+    P  CRS+L +    P D Y YG+ S++  L +A +  S+I   LDR     
Sbjct: 36  SNICRFAPDPSYCRSVLPN---QPGDIYSYGRLSLRRSLSRARRFISMIDAELDR-KGKV 95

Query: 90  LNQREAGALEDCRDLSDSNVEFLRSIESVLEAAKSVDEELVERVQSILSAIVTNGQTCID 149
             +   GALEDC+ L+   +++L S     ++ K++     E V + LSA +TN QTC++
Sbjct: 96  AAKSTVGALEDCKFLASLTMDYLLSSSQTADSTKTLSLSRAEDVHTFLSAAITNEQTCLE 155

Query: 150 GLVESRSSLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKG-------DGGDSAA 209
           GL  + S   N L+  + +  +LY VSL L     S+ W  RR +               
Sbjct: 156 GLKSTASE--NGLSGDLFNDTKLYGVSLAL----FSKGWVPRRQRSRPIWQPQARFKKFF 215

Query: 210 GARN-REPLNTLIKGLHKMGSCNQSTGCSGRKRLLADLGTTGIFINDTVVVSSTGADNFT 269
           G RN + PL        KM    ++   +  +R L       + ++D V V   G  NFT
Sbjct: 216 GFRNGKLPL--------KMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFT 275

Query: 270 SIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVGDGINRTVITGNHSV 329
           +I  AIA APN +   +GYF+IYV  G YEEYV VPK K  +M++GDGIN+TVITGN SV
Sbjct: 276 TINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSV 329

Query: 330 VDGWTTYNSSTF 334
           VDGWTT+NS+TF
Sbjct: 336 VDGWTTFNSATF 329

[Close]

BLAST of Cp4.1LG09g03490 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 164.1 bits (414), Expect = 2.2e-40
Identity = 119/345 (34.49%), Postives = 182/345 (52.75%), Query Frame = 0


Query: 29  SADLCKSTLYPKLCRSILSSIRFSPSDPYGYGKFSVKNCLKQAIKMSSVIGDYLDRGSDS 88
           S+ +CK+T  PK C+S+      S  +   YG FS++  L Q+ K    +  Y+ R  ++
Sbjct: 28  SSTICKTTPDPKYCKSVFP---HSQGNVQQYGCFSIRKSLSQSRKFIRTVDRYIKR--NA 87

Query: 89  RLNQREA-GALEDCRDLSDSNVEF-LRSIESVLEAA-----KSVDEELVERVQSILSAIV 148
            L+Q     AL+DCR L+   +++ L S E+V + +     K +     + +Q++LSA +
Sbjct: 88  HLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFKPLSFPKADDIQTLLSAAL 147

Query: 149 TNGQTCIDGLVESRS-----SLGNALTAPMSSAGQLYSVSLGLVTNSLSRLWKKRRMKGD 208
           TN QTC++GL  + S     ++   +  P+ +  +L  VSL L T       KKR     
Sbjct: 148 TNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLALFTKGWVPKKKKRAGFAW 207

Query: 209 GGDSAAGARNREPLNTLIKGL--HKMGSCNQSTGCSGRKRLLADLGTTG-------IFIN 268
               +  + + +P      G    KM    ++   S  +R LAD  + G       + I+
Sbjct: 208 AQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKLADGDSNGDGDDGSMVLIS 267

Query: 269 DTVVVSSTGADNFTSIGDAIAFAPNNSMPQDGYFVIYVRGGYYEEYVVVPKYKTNIMLVG 328
           D V VS  G  NFT+I  A+A APNN+    G+F+IYV  G YEEY+ + K K  +M++G
Sbjct: 268 DIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIG 327

Query: 329 DGINRTVITGNHSVVDGWTTYNSSTFRANTSLRVDWPGYSLMNIT 353
           DGIN+TV+TGN SVVDGWTT+NS+TF       V  P +  +NIT
Sbjct: 328 DGINQTVVTGNRSVVDGWTTFNSATF------AVTAPNFVAVNIT 361

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g03490 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94CB1	8.2e-80	35.50	Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF77	4.2e-76	34.55	Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3...	[more]
Q8RXK7	2.9e-45	36.14	Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3...	[more]
O22256	1.0e-42	37.50	Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3...	[more]
Q9SRX4	3.1e-39	34.49	Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=37...	[more]

BLAST of Cp4.1LG09g03490 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541032.1	6.42e-241	67.26	probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita pepo subsp. pepo]	[more]
XP_022945508.1	1.06e-239	66.73	probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita moschata] >XP_022...	[more]
KAG6573907.1	2.97e-237	66.61	putative pectinesterase/pectinesterase inhibitor 25, partial [Cucurbita argyrosp...	[more]
XP_022968171.1	1.59e-236	66.37	probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima]	[more]
XP_022976564.1	1.62e-195	57.14	probable pectinesterase/pectinesterase inhibitor 25 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG09g03490 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G137	5.12e-240	66.73	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111449722 PE=3 SV=1	[more]
A0A6J1HU46	7.72e-237	66.37	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467487 PE=3 SV=1	[more]
A0A6J1INW0	7.84e-196	57.14	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111476893 PE=3 SV=1	[more]
A0A6J1D8L4	1.79e-190	56.81	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111018622 PE=3 SV=1	[more]
A0A6J1GYC3	9.25e-188	56.14	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458594 PE=3 SV=1	[more]

BLAST of Cp4.1LG09g03490 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10720.2	5.8e-81	35.50	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G04970.1	3.0e-77	34.55	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G02330.1	2.1e-46	36.14	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT2G47550.1	7.4e-44	37.50	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G02810.1	2.2e-40	34.49	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 24..179 e-value: 3.0E-23 score: 93.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 29..180 e-value: 2.0E-10 score: 38.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 31..178 e-value: 1.9E-20 score: 73.7
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 250..336 e-value: 1.9E-22 score: 79.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 30..183 e-value: 3.1E-25 score: 90.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 31..182
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 244..338 e-value: 6.0E-21 score: 77.1
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 335..380 coord: 12..333
None	No IPR available	PANTHER	PTHR31707:SF231	PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATED	coord: 12..333
None	No IPR available	PANTHER	PTHR31707:SF231	PECTINESTERASE/PECTINESTERASE INHIBITOR 47-RELATED	coord: 335..380
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 32..182 e-value: 1.77307E-29 score: 109.45
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 246..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g03490.1	Cp4.1LG09g03490.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity