Cp4.1LG09g02780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g02780
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCTL-like protein DDB_G0274487
LocationCp4.1LG09: 1676550 .. 1680551 (+)
RNA-Seq ExpressionCp4.1LG09g02780
SyntenyCp4.1LG09g02780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAAGAATATGAGTTGAACGCATGATTGATTGTCCATTAGTGGAGGTGTCCGGAGTGATTCTGTCCAAACTTCTGATACTTGTTTTCTGTAAAGAAAGCCCTAAAAGGCAACTGCTTCATCCCTTCCCCCTGTCGTTTTCTTTACCCACTTTTCACGTTCTTCTCAACCTCATTATGTTTCAGTTTAAAATCCTCCATTTCTCAAACCCATTTCAAAACAATGCTCCTTAGTTGTTAATCCGTTCGCTATGGGCGACTCCGGCAGCGTCTCCTCTTCTTCATTGGATTCCGCCTCCGTTGCCACCGCCGATCAGGTCCAGGTAAATTCAACTAATCTGCTTTGTTCTGTTCAATTTCATGTTCTTACTGCCTTTTTCCTCACTGGGTTTCGTTTACTTTACTGATAACTGATCAGAATGCAGCTCGGGGTCGAGGCCAAGCGTTGTCGAGTGAATCGTCACGTTCTTGGCGGGATATTTTCTGGTCCATTGTGTTTATAATTCACTTGATCATTGTGGGATTTGTGCTCGTGATTCTGGGGCTTAATAGGTTCAAGAAGAGCAATAGGTTACAAATCGATAAGTTCACCAATACAATTATGGAGAATCGGGTTGGATTGACAGAGGATTACTGGCCGCTTTTTGCTTTGGCAGGTGGAGTTGGAACCTTACTTGGCTGGACTTGGTTGTTCTTGCTTGGTTCTTTTGCAAATCATGTAATGAAAATTTCTGTGCATATTTTGACTACTTATCTTGCTGTGATAAGTGTATTGTGCTTCTGGGGCCAGCTTTTTTTCTGGGGTGTAGCGTTTGCAATCGGTGCAGGGCTGCAGTTCCTGTATGTTATATCAGTTATAGACAGGTACGAGCTTGAACCATGAAGATTTTTGTTCTTCTTGAGCTTGATTGAGCTTGATTTATTGTCGTGTTTATGAAATTCTCCTGCTTTTGGTTAGGAATAACGACTCTCTACAATAGTATGATATTGTCCGCTTTGAGCATAAGTTCTCATGGCTTTGCTTTGGGCTTTACCGAGAGTATTCCTCACTTATAAACCCATGATCTTCTACTAGATTAGTCAAGGTGGGAGTCCCTCCCAACATTCCTCAACAATACTCCCCAACAAAGTGTACCATAGAATCTCCCTTGAGGTTTATAGAACCCTCGAACAGCCTCTCCTTAATCAAGGTTCAACACCTTCTCTAGAGTCCTACATCGGCTAATTAAGGAGTTGATTATGAGTTTATAAGTAAGGAATACATCTCAATTGGTATGAGGCCTTTTGGGGAAACCAAAAGTAAAGTCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGGTATGTGATTCCTAACATGGTATCAGAGTCATACCCTTAGCTTAGCCATGTCAATAGAATCCTCAAATGTCGAATAAAGAAGTTGTGAGCCTCAAAGGTGTAGTCAAAAGTGACTCAAGTGTCAAACAAAGGGTTTACTTTGTTCGAGGGCTCCAGAGAAAGAGTCGAGCCTTGATTATGGGAAAGTTGTTCGAGAACTCCATAGGCTTCAGGGGAGGCTCTATGGTGTGCTTTGTTCGAGGGGAGGATTGTTGGGAGAGGAGTCCTACATCGACTAATTAAGGAGTTGATTGTGAGTTTATAAGTAAGGAATGCATCTCCATTGGTATGAGGCCTTTAGGGGAAACCAAAAGTAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGATCTGTGATTCCTAACACTTTCCCCTCTTAACTCACCTTTATGAGAATTAGAAATGCAGAAGAAAAACATATTAGATTAGGTGATCATTGCAGTCTTCTCTGATCATGCATTTTTGCTTCCATAGACTACCATTTACATTGCTAGTGTTGCAAAAGGCTGTAATGATGGTATCCGGGCTACCCGAAGTTATTAGAGTTGCGTATACTTTCATGATTGTCATGCTTTTGTGTATGGGAGTTTGGTCTTTCGGAGTCGCTGGTATCGTGGCTTCGAGTATGGGGGACGGTGGACGCTGGTGGCTAATTGTGGTGAGCTCACAAGTTTTCTCTTTCTTATGTAGCTTTAGGTGGAACATTAGCTGAGTTTCTATGATTTGGCATTTCTATTTGCAGGTTTTCTCCATAAGCTTATTCTGGGTTGGTGCTGTTTTTTGTAATACTCTACATGTTATAGTTTCTGGAATGGTGTTTCTAGTTCTATTCCATGGCGATGGAGAATCATCATCTTTGCCATCAAAGTCATTGGTTCGGGCTTCACGGTATGCTGTGACGACATCTTTTGGTAGCATTTGCTATGGATCTCTTTTTACAGCAGCCATTCGGACTCTGCGGTGGGAGGTAGGTACTCATGGAAGCTCTTTATTTCGTCTCTCCCTGCACGTTGATAAGCTGTATGTTACCTTTTTAGATCAGGGGAATTCGATCGAAGATCGGCAAGAACGAGTGTCTACTTTGTTGTGTTGATTTTTTGTTTCATCTCGTCGAGACTCTTGTTCGTTTCTTTAACAAATATGCGTATGTCCAGGTATTCCCTTTTAGCTAATGTCGATTCGAATAATTGATGCTGATTCGTACTCCGAATTTTATGAATTTCAGTATGGTTGCACTGCTTGTTTTTGCCTCAGATAGCAGTTTATGGTAAAAGCTTCAACCGTTCAGCTAGGGATGCGTGGGAGCTGTTCCAGTCAACTGGAGTTGAAGCTCTTATTGCTTATGATTGCTCAGGTGCTGTTCTGCTGATGAGCACCATGATGGGCGGGCTCGTTGCTGGGACTTGCTCGGGCATTTGGACATGGTTTAAGTGGAAGGATAAGGTGTCGATGGTGGCGTGTACCGCAACATTGATGGGAATGGTTATGGTAAATTCTATGCAACTTTTTTGCTTGATGATTCTAGTCTATGAATGGAGGTTTATGTTACAGCCATTTGTAACGGCCCAAGCCCACCGTTAGCAGATATTGTCCTCTTTGAGATTTCCTTATAAAGAATGACTCGTTCTCCTCCCCAAGCAATGTGGATCTCACAATCCACCCCCTTCGAGGACCAGCATCCTCACTAGCACTCGTTCCCTTCTCTAATCGATGTGGGACCCCTCAATCTACCTCCTTCGTGACCCAGCATCCTTCTGGCACACCGCCTCGTGTCCACCCCCCTTCAGGACTCAGCCTCCTTGCTAGCACATCACCCGTTGTCTGGCTCCGATACCATGTGTAACGGCTCAAGCCTACCGCTAGCAGGATTTTGTCCTATGTTTCTTTCTCCTCCCCAACCGATGTGGGATCTCACAATCCACCTACCTCCCTTCGGGCCCAGCATCCTTGCTGGCACTCTTTCCCTTCTCCCATCAATGTGGGACCCCCCAATCCAGGATCTCAAAATCCACTCCTCCCTTTAGGGCCTGCATCCTCACTGGTACTCGTTCCCTTCTCTAATCGATGTGGAACCCCCCAATCTACACCCCTTCGGGCCCAGTGTCCTTGTTGACACACTACCTCGTGTCCACCCCCCTTCAAGGCTCAGCCTCCTCGCTGGCACATCGCCCGGTGTCTGGCTCTGATACCATTTGTAACGGCTCAAGCCCACCGCTAGCAGGATATTGTCGTCTTTGGGCTTTCCCCTGAAAGTTTCTAAAACGCGTATACTAGGAAGAGATTTCAACACCCTTATAAAGAATGTTTCGTTCTCCTCTCCAATCGATGTGAGATCTCACACTTTATAACTCCACTAATCAAGTTTTGGTTCTAATTTTCAGGCGGGCCTTGCAATAGTGGTCGTGGAAAGTGCCGTTACATCCATATACATCTGTTATGCGGAAGATCCATTGCTGATTCACAGATGGGATATTGAATTCTTCGACCAGATGTCGGAGATGCTTCACCAGCGCCTTCAGCATCGGAGTGCACGAGCAAGGGAAGTGTTAGCGCAGTATCGGTTCGACAGCCGCAGAGAAGAACTGGCACAGGCATGA

mRNA sequence

CAAAGAATATGAGTTGAACGCATGATTGATTGTCCATTAGTGGAGGTGTCCGGAGTGATTCTGTCCAAACTTCTGATACTTGTTTTCTGTAAAGAAAGCCCTAAAAGGCAACTGCTTCATCCCTTCCCCCTGTCGTTTTCTTTACCCACTTTTCACGTTCTTCTCAACCTCATTATGTTTCAGTTTAAAATCCTCCATTTCTCAAACCCATTTCAAAACAATGCTCCTTAGTTGTTAATCCGTTCGCTATGGGCGACTCCGGCAGCGTCTCCTCTTCTTCATTGGATTCCGCCTCCGTTGCCACCGCCGATCAGGTCCAGAATGCAGCTCGGGGTCGAGGCCAAGCGTTGTCGAGTGAATCGTCACGTTCTTGGCGGGATATTTTCTGGTCCATTGTGTTTATAATTCACTTGATCATTGTGGGATTTGTGCTCGTGATTCTGGGGCTTAATAGGTTCAAGAAGAGCAATAGGTTACAAATCGATAAGTTCACCAATACAATTATGGAGAATCGGGTTGGATTGACAGAGGATTACTGGCCGCTTTTTGCTTTGGCAGGTGGAGTTGGAACCTTACTTGGCTGGACTTGGTTGTTCTTGCTTGGTTCTTTTGCAAATCATGTAATGAAAATTTCTGTGCATATTTTGACTACTTATCTTGCTGTGATAAGTGTATTGTGCTTCTGGGGCCAGCTTTTTTTCTGGGGTGTAGCGTTTGCAATCGGTGCAGGGCTGCAGTTCCTGTATGTTATATCAGTTATAGACAGACTACCATTTACATTGCTAGTGTTGCAAAAGGCTGTAATGATGGTATCCGGGCTACCCGAAGTTATTAGAGTTGCGTATACTTTCATGATTGTCATGCTTTTGTGTATGGGAGTTTGGTCTTTCGGAGTCGCTGGTATCGTGGCTTCGAGTATGGGGGACGGTGGACGCTGGTGGCTAATTGTGGTTTTCTCCATAAGCTTATTCTGGGTTGGTGCTGTTTTTTGTAATACTCTACATGTTATAGTTTCTGGAATGGTGTTTCTAGTTCTATTCCATGGCGATGGAGAATCATCATCTTTGCCATCAAAGTCATTGGTTCGGGCTTCACGGTATGCTGTGACGACATCTTTTGGTAGCATTTGCTATGGATCTCTTTTTACAGCAGCCATTCGGACTCTGCGGTGGGAGATCAGGGGAATTCGATCGAAGATCGGCAAGAACGAGTGTCTACTTTGTTGTGTTGATTTTTTGTTTCATCTCGTCGAGACTCTTGTTCGTTTCTTTAACAAATATGCGTATGTCCAGATAGCAGTTTATGGTAAAAGCTTCAACCGTTCAGCTAGGGATGCGTGGGAGCTGTTCCAGTCAACTGGAGTTGAAGCTCTTATTGCTTATGATTGCTCAGGTGCTGTTCTGCTGATGAGCACCATGATGGGCGGGCTCGTTGCTGGGACTTGCTCGGGCATTTGGACATGGTTTAAGTGGAAGGATAAGGTGTCGATGGTGGCGTGTACCGCAACATTGATGGGAATGGTTATGATGTCGGAGATGCTTCACCAGCGCCTTCAGCATCGGAGTGCACGAGCAAGGGAAGTGTTAGCGCAGTATCGGTTCGACAGCCGCAGAGAAGAACTGGCACAGGCATGA

Coding sequence (CDS)

ATGGGCGACTCCGGCAGCGTCTCCTCTTCTTCATTGGATTCCGCCTCCGTTGCCACCGCCGATCAGGTCCAGAATGCAGCTCGGGGTCGAGGCCAAGCGTTGTCGAGTGAATCGTCACGTTCTTGGCGGGATATTTTCTGGTCCATTGTGTTTATAATTCACTTGATCATTGTGGGATTTGTGCTCGTGATTCTGGGGCTTAATAGGTTCAAGAAGAGCAATAGGTTACAAATCGATAAGTTCACCAATACAATTATGGAGAATCGGGTTGGATTGACAGAGGATTACTGGCCGCTTTTTGCTTTGGCAGGTGGAGTTGGAACCTTACTTGGCTGGACTTGGTTGTTCTTGCTTGGTTCTTTTGCAAATCATGTAATGAAAATTTCTGTGCATATTTTGACTACTTATCTTGCTGTGATAAGTGTATTGTGCTTCTGGGGCCAGCTTTTTTTCTGGGGTGTAGCGTTTGCAATCGGTGCAGGGCTGCAGTTCCTGTATGTTATATCAGTTATAGACAGACTACCATTTACATTGCTAGTGTTGCAAAAGGCTGTAATGATGGTATCCGGGCTACCCGAAGTTATTAGAGTTGCGTATACTTTCATGATTGTCATGCTTTTGTGTATGGGAGTTTGGTCTTTCGGAGTCGCTGGTATCGTGGCTTCGAGTATGGGGGACGGTGGACGCTGGTGGCTAATTGTGGTTTTCTCCATAAGCTTATTCTGGGTTGGTGCTGTTTTTTGTAATACTCTACATGTTATAGTTTCTGGAATGGTGTTTCTAGTTCTATTCCATGGCGATGGAGAATCATCATCTTTGCCATCAAAGTCATTGGTTCGGGCTTCACGGTATGCTGTGACGACATCTTTTGGTAGCATTTGCTATGGATCTCTTTTTACAGCAGCCATTCGGACTCTGCGGTGGGAGATCAGGGGAATTCGATCGAAGATCGGCAAGAACGAGTGTCTACTTTGTTGTGTTGATTTTTTGTTTCATCTCGTCGAGACTCTTGTTCGTTTCTTTAACAAATATGCGTATGTCCAGATAGCAGTTTATGGTAAAAGCTTCAACCGTTCAGCTAGGGATGCGTGGGAGCTGTTCCAGTCAACTGGAGTTGAAGCTCTTATTGCTTATGATTGCTCAGGTGCTGTTCTGCTGATGAGCACCATGATGGGCGGGCTCGTTGCTGGGACTTGCTCGGGCATTTGGACATGGTTTAAGTGGAAGGATAAGGTGTCGATGGTGGCGTGTACCGCAACATTGATGGGAATGGTTATGATGTCGGAGATGCTTCACCAGCGCCTTCAGCATCGGAGTGCACGAGCAAGGGAAGTGTTAGCGCAGTATCGGTTCGACAGCCGCAGAGAAGAACTGGCACAGGCATGA

Protein sequence

MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTATLMGMVMMSEMLHQRLQHRSARAREVLAQYRFDSRREELAQA
Homology
BLAST of Cp4.1LG09g02780 vs. ExPASy Swiss-Prot
Match: Q869R1 (CTL-like protein DDB_G0274487 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0274487 PE=3 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.0e-24
Identity = 105/364 (28.85%), Postives = 180/364 (49.45%), Query Frame = 0

Query: 42  WRDIFWSIVFIIHLIIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFA 101
           ++DI++SI+F ++      +L+++  + F K+     D   +   +N  G +  +  LFA
Sbjct: 135 YQDIWFSILFGLNF----GLLIVVSASAFAKTPYTYYD---SNYYDNTSGGSFGF--LFA 194

Query: 102 LAGGV---GTLLGWTWLFLLGSFANHVMKISVHILTTYLAVISVLCF-WGQLFFWGVAFA 161
           +         L  W WL L  + A  ++K S       +    V  F WG ++  G+ FA
Sbjct: 195 ILPFTIVFSLLYIWAWLKLAANHAESLIKYSFFGAMGLMIGYCVFFFVWGAIYL-GIIFA 254

Query: 162 IGAGLQFLYVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVA 221
           I A    L+ IS   R+PFT  +L  AV ++   P VIR  Y  + +  +   VW    A
Sbjct: 255 IMAFFIILFYISCRSRIPFTATLLSNAVAIIKEYPSVIRAGYVSIFINFVWFIVWGSAFA 314

Query: 222 GIVASSMGDGGRWWLIVVFSI-SLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSK 281
            +  + +  G     I ++ + +L+WV  V  NTLH  VSG++    F        +P  
Sbjct: 315 RV--NMVYTGAIQTCINIYLVFTLYWVFHVIKNTLHTTVSGLLATWYFCSGPNGVGMPHN 374

Query: 282 SLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFH---- 341
             + ++R A+TTSFGSIC+GSL  + I TLR+     +  I     ++  + ++F+    
Sbjct: 375 PTLGSARRALTTSFGSICFGSLIISLIETLRYL---SQMMINNRNVVVKIIGYIFNCILS 434

Query: 342 LVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMG 397
           ++ ++V+FFN YA+  +A+YGKSF  SA+  + +F++     +I  +  G  +     +G
Sbjct: 435 MLSSIVQFFNTYAFTHVAIYGKSFCDSAKSTFTMFENRLGSTIINDNFVGTTI----AIG 479

BLAST of Cp4.1LG09g02780 vs. ExPASy Swiss-Prot
Match: P0CM93 (Protein PNS1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-3501A) OX=283643 GN=PNS1 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-14
Identity = 88/373 (23.59%), Postives = 166/373 (44.50%), Query Frame = 0

Query: 44  DIFWSIVFIIHLIIVGFVLVI-LGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFAL 103
           DI   I+FI    +VGF +V  + ++ F + N L      ++I      +T DY  ++ L
Sbjct: 94  DIIPLILFI--AAVVGFAVVSGIAIHGFVQVNGLGGGMGDSSIGRTGSSITLDYHTVYLL 153

Query: 104 --AGGVGTLLGWTWLFLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGA 163
                +G ++   +L  L +F   ++++++ +       I +  F  Q +   + F I A
Sbjct: 154 LVVVALGLVIASLYLAALRAFTKIILEVTLALTVILNIGICIYYFIIQYWSGAIIFLIIA 213

Query: 164 GLQFLYVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTFMIVM--------LLCMGV- 223
            +   +   +  R+P   L+LQ  + +    P V  V +  +I+           C+ + 
Sbjct: 214 LVSVFFYWGMRKRIPLAKLLLQTTIDVTKHHPSVYVVVFIGLIIQAAVSVWYTFTCIAIY 273

Query: 224 --WSFGVAGIVASSMGDGGRWWLIVVFSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGE 283
             W+ G A              L+   + S  W+  V  N +   ++G VF   ++    
Sbjct: 274 VKWTPGSAACSDGGCSSSKVAGLVFYATFSYLWLSQVIGNVILCTLAGGVFGGWYYYGPR 333

Query: 284 S--SSLPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRS-KIGKNE----C 343
           +    +P ++ + A   A T S GSI +GSL    +  LR  ++  R  + G+ +     
Sbjct: 334 TPGGGVPKRASLLAFVRASTLSLGSIAFGSLLVTILELLRLILQLFRQYEAGQGDMIGSI 393

Query: 344 LLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCSG 396
           L+C        ++ +V +FNKYAY++IA+YGKS+  +A+D W L +  G++AL+     G
Sbjct: 394 LICIAQCCIGCIQWMVEYFNKYAYIEIALYGKSYIPAAKDTWRLLKDRGIDALVNDSLVG 453

BLAST of Cp4.1LG09g02780 vs. ExPASy Swiss-Prot
Match: P0CM92 (Protein PNS1 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC MYA-565) OX=214684 GN=PNS1 PE=3 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.1e-14
Identity = 88/373 (23.59%), Postives = 166/373 (44.50%), Query Frame = 0

Query: 44  DIFWSIVFIIHLIIVGFVLVI-LGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFAL 103
           DI   I+FI    +VGF +V  + ++ F + N L      ++I      +T DY  ++ L
Sbjct: 94  DIIPLILFI--AAVVGFAVVSGIAIHGFVQVNGLGGGMGDSSIGRTGSSITLDYHTVYLL 153

Query: 104 --AGGVGTLLGWTWLFLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGA 163
                +G ++   +L  L +F   ++++++ +       I +  F  Q +   + F I A
Sbjct: 154 LVVVALGLVIASLYLAALRAFTKIILEVTLALTVILNIGICIYYFIIQYWSGAIIFLIIA 213

Query: 164 GLQFLYVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTFMIVM--------LLCMGV- 223
            +   +   +  R+P   L+LQ  + +    P V  V +  +I+           C+ + 
Sbjct: 214 LVSVFFYWGMRKRIPLAKLLLQTTIDVTKHHPSVYVVVFIGLIIQAAVSVWYTFTCIAIY 273

Query: 224 --WSFGVAGIVASSMGDGGRWWLIVVFSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGE 283
             W+ G A              L+   + S  W+  V  N +   ++G VF   ++    
Sbjct: 274 VKWTPGSAACSDGGCSSSKVAGLVFYATFSYLWLSQVIGNVILCTLAGGVFGGWYYYGPR 333

Query: 284 S--SSLPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRS-KIGKNE----C 343
           +    +P ++ + A   A T S GSI +GSL    +  LR  ++  R  + G+ +     
Sbjct: 334 TPGGGVPKRASLLAFVRASTLSLGSIAFGSLLVTILELLRLILQLFRQYEAGQGDMIGSI 393

Query: 344 LLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCSG 396
           L+C        ++ +V +FNKYAY++IA+YGKS+  +A+D W L +  G++AL+     G
Sbjct: 394 LICIAQCCIGCIQWMVEYFNKYAYIEIALYGKSYIPAAKDTWRLLKDRGIDALVNDSLVG 453

BLAST of Cp4.1LG09g02780 vs. ExPASy Swiss-Prot
Match: Q870V7 (Protein PNS1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=pns-1 PE=3 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.9e-14
Identity = 91/359 (25.35%), Postives = 155/359 (43.18%), Query Frame = 0

Query: 47  WSIVFIIHLIIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGV 106
           W    +    + GFV V          NR Q +   N    N  GLT     LF      
Sbjct: 105 WWAGLLFLATVAGFVAVSAISIHGYADNRSQNNGSLNG-QRNTFGLTTHTIYLFVWVLIC 164

Query: 107 GTLLGWTWLFLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFF-WGVAFAIGAGLQFL 166
             +L + ++++   F    +  +  IL   + +++ L    + ++  G+ F I   LQ L
Sbjct: 165 AIVLSYAYMWMARKFTKQFI-YATGILNIVMGLVTALYMLSRKYWSGGIVFLIFVVLQAL 224

Query: 167 YVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIV----- 226
           +  S   R+PF+ L+LQ A+ +      V  V+    ++  L    W+  +  +      
Sbjct: 225 FFWSCRSRIPFSTLMLQTAIDVSKVHGHVYLVSAVGGVIGTLFAAYWAITLVAVYVKFEP 284

Query: 227 ---------ASSMGDGGRWWLIVVFSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESS 286
                    A     G    LIV  + + +W+     NT+H  V+G+     F+    S 
Sbjct: 285 DPNNAACRNAGGCSSGKVIGLIVFITFAGYWISEWLKNTIHTTVAGIYGSWYFN----SR 344

Query: 287 SLPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRSKIGKNECLL-----CC 346
           + P+K    A + ++T SFGSI  GSLF A I  +R   +  +    +   +L     C 
Sbjct: 345 NYPTKVTRGALKRSLTYSFGSISLGSLFIAIINLIRQLAQAAQQNAAQEGDILGTILWCI 404

Query: 347 VDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCSGAVL 386
              L  +++ LV F N+YA+  IA+YGK++  +A+D W++ +  G++ALI     G VL
Sbjct: 405 FGCLIGILDWLVEFINRYAFCHIALYGKAYFAAAKDTWKMVKDRGIDALINECLIGPVL 457

BLAST of Cp4.1LG09g02780 vs. ExPASy Swiss-Prot
Match: Q4I8E9 (Protein PNS1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 / NRRL 31084 / PH-1) OX=229533 GN=PNS1 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.6e-13
Identity = 87/379 (22.96%), Postives = 162/379 (42.74%), Query Frame = 0

Query: 47  WSIVFIIHLIIVGFVLVI-LGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGG 106
           W+ + +I L+  GFV+V  L L  +  +     D   N   +N          LF     
Sbjct: 90  WAGILLI-LVFAGFVVVSGLALQGYSANKGNAGDGIYNN--KNDFSPNTSTVILFMFVLA 149

Query: 107 VGTLLGWTWLFLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFL 166
           V  +L + ++++   F    + ++  +   +    ++   W + +  G+ F I       
Sbjct: 150 VAFVLSYAYVWMARLFPKQFIWVTGILNVCWAIGTAIFYLWRKYWSAGIVFLIFGLFMAF 209

Query: 167 YVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVAS--- 226
              + I R+PF+ L+L+  + +      V  V+    I+       ++  + GI      
Sbjct: 210 CFWTWISRIPFSALMLKTTIDVSKKYGHVYLVSLIGGIIATAFSAWYAITLVGIYVKYQP 269

Query: 227 -----SMGDGGRW-----WLIVVFSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSS 286
                S  DGG        LI   + +++W      NT+H  ++G+     F+      +
Sbjct: 270 AQDNPSCADGGCGKGKVIGLIAFITFAMYWFSEWLKNTIHTTIAGVYGSWYFN----PHN 329

Query: 287 LPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRSKIGKN-----ECLLCCV 346
            P  +   +++ A+T SFGSI  GSL  A I+ LR      R++ G +       + CC+
Sbjct: 330 FPKDATRASAKRALTYSFGSIALGSLLVAIIQFLRQICNAARNQEGADGSFVGYAIFCCI 389

Query: 347 DFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCSGAVLLM 406
             L  L+E  V F N+YA+  IA+YGK++  +A+D W++ +  G++ALI     G VL  
Sbjct: 390 SCLLGLLEWAVEFINRYAFCHIALYGKAYFAAAKDTWKMIKDRGIDALINDCLIGPVLSF 449

BLAST of Cp4.1LG09g02780 vs. NCBI nr
Match: XP_023542360.1 (CTL-like protein DDB_G0274487 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 868 bits (2244), Expect = 0.0
Identity = 461/497 (92.76%), Postives = 461/497 (92.76%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60
           MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF
Sbjct: 1   MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60

Query: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120
           VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS
Sbjct: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120

Query: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLV 180
           FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLV
Sbjct: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLV 180

Query: 181 LQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240
           LQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL
Sbjct: 181 LQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240

Query: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300
           FWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT
Sbjct: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300

Query: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360
           AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA
Sbjct: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360

Query: 361 RDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTAT 420
           RDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTAT
Sbjct: 361 RDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTAT 420

Query: 421 LMGMVM------------------------------------MSEMLHQRLQHRSARARE 461
           LMGMVM                                    MSEMLHQRLQHRSARARE
Sbjct: 421 LMGMVMAGLAIVVVESAVTSIYICYAEDPLLIHRWDIEFFDQMSEMLHQRLQHRSARARE 480

BLAST of Cp4.1LG09g02780 vs. NCBI nr
Match: KAG6573984.1 (CTL-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013042.1 hypothetical protein SDJN02_25798, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 852 bits (2200), Expect = 7.04e-310
Identity = 451/497 (90.74%), Postives = 455/497 (91.55%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60
           M DSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF
Sbjct: 1   MADSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60

Query: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120
           VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS
Sbjct: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120

Query: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLV 180
           FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFA GAGLQFLYVISVIDRLPFTLLV
Sbjct: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFASGAGLQFLYVISVIDRLPFTLLV 180

Query: 181 LQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240
           LQKAV MVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL
Sbjct: 181 LQKAVTMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240

Query: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300
           FWVGAVFCNTLHVIVSGMVFLVL HGDGESSSLPSKSLV ASRYAVTTSFGSICYGSLFT
Sbjct: 241 FWVGAVFCNTLHVIVSGMVFLVLCHGDGESSSLPSKSLVLASRYAVTTSFGSICYGSLFT 300

Query: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360
           AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA
Sbjct: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360

Query: 361 RDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTAT 420
           RDAWELFQSTGVEAL+AYDCSGAVLLMST+MGGL+AGTCSGIWTWFKWKDKVSMVACTAT
Sbjct: 361 RDAWELFQSTGVEALVAYDCSGAVLLMSTIMGGLIAGTCSGIWTWFKWKDKVSMVACTAT 420

Query: 421 LMGMVM------------------------------------MSEMLHQRLQHRSARARE 461
           LMGM+M                                    MSEMLHQRLQHRSARARE
Sbjct: 421 LMGMIMAGLAIVVVESAVTSIYICYAEDPLLIHRWDIEFFDQMSEMLHQRLQHRSARARE 480

BLAST of Cp4.1LG09g02780 vs. NCBI nr
Match: XP_022945781.1 (CTL-like protein DDB_G0274487 [Cucurbita moschata])

HSP 1 Score: 852 bits (2201), Expect = 8.08e-310
Identity = 451/496 (90.93%), Postives = 454/496 (91.53%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60
           M DSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF
Sbjct: 1   MADSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60

Query: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120
           VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS
Sbjct: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120

Query: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLV 180
           FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFA GAGLQFLYVISVIDRLPFTLLV
Sbjct: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFASGAGLQFLYVISVIDRLPFTLLV 180

Query: 181 LQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240
           LQKAV MVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL
Sbjct: 181 LQKAVTMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240

Query: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300
           FWVGAVFCNTLHVIVSGMVFLVLFHG GESSSLPSKSLVRASRYAVTTSFGSICYGSLFT
Sbjct: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGGGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300

Query: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360
           AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA
Sbjct: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360

Query: 361 RDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTAT 420
           RDAWELFQSTGVE L+AYDCSGAVLLMST+MGGL+AGTCSGIWTWFKWKDKVSMVACTAT
Sbjct: 361 RDAWELFQSTGVETLVAYDCSGAVLLMSTIMGGLIAGTCSGIWTWFKWKDKVSMVACTAT 420

Query: 421 LMGMVM------------------------------------MSEMLHQRLQHRSARARE 460
           LMGMVM                                    MSEMLHQRLQHRSARARE
Sbjct: 421 LMGMVMAGLAIVVVESAVTSIYICYAEDPLLIHRWDIEFFDQMSEMLHQRLQHRSARARE 480

BLAST of Cp4.1LG09g02780 vs. NCBI nr
Match: XP_022968496.1 (CTL-like protein DDB_G0274487 [Cucurbita maxima])

HSP 1 Score: 845 bits (2183), Expect = 4.63e-307
Identity = 447/494 (90.49%), Postives = 451/494 (91.30%), Query Frame = 0

Query: 3   DSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGFVL 62
           DSGSVSSSS DSASVATADQ+QNAARGRGQALSSES RSWRDIFWSIVFIIHLIIVGFVL
Sbjct: 4   DSGSVSSSSFDSASVATADQLQNAARGRGQALSSESPRSWRDIFWSIVFIIHLIIVGFVL 63

Query: 63  VILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFA 122
           VILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFA
Sbjct: 64  VILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFA 123

Query: 123 NHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQ 182
           NHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQ
Sbjct: 124 NHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQ 183

Query: 183 KAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFW 242
           KAV MVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFW
Sbjct: 184 KAVKMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFW 243

Query: 243 VGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFTAA 302
           VGAVFCNTLHVIVSGMVFLVLFHG GESSSLPSKSL  ASRYAVTTSFGSICYGSLFTAA
Sbjct: 244 VGAVFCNTLHVIVSGMVFLVLFHGGGESSSLPSKSLAWASRYAVTTSFGSICYGSLFTAA 303

Query: 303 IRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARD 362
           IRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARD
Sbjct: 304 IRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARD 363

Query: 363 AWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTATLM 422
           AWELFQSTGVEAL+AYDCSGAVLLMST+MGGL+AGTCSGIWTWFKWKDKVSMVACTATLM
Sbjct: 364 AWELFQSTGVEALVAYDCSGAVLLMSTVMGGLIAGTCSGIWTWFKWKDKVSMVACTATLM 423

Query: 423 GMVM------------------------------------MSEMLHQRLQHRSARAREVL 460
           GMVM                                    MSEMLHQRLQHRSARAREVL
Sbjct: 424 GMVMAGLAIVVVESAVTSIYICYAEDPLLIHRWDIEFFDQMSEMLHQRLQHRSARAREVL 483

BLAST of Cp4.1LG09g02780 vs. NCBI nr
Match: XP_038891301.1 (CTL-like protein DDB_G0274487 [Benincasa hispida])

HSP 1 Score: 806 bits (2082), Expect = 7.98e-292
Identity = 423/502 (84.26%), Postives = 443/502 (88.25%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGR-----GQALSSESSRSWRDIFWSIVFIIHL 60
           MGDSGSVSSSS DSASV TADQ QN A+GR     GQALS ESSRSWRD+FWS VF+IHL
Sbjct: 1   MGDSGSVSSSSFDSASVVTADQAQNVAQGRMSGDGGQALSDESSRSWRDVFWSFVFMIHL 60

Query: 61  IIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWL 120
           I VGFVLV+LGLNRFKKSNRLQIDK+TNTIMENRVGLTEDYWPL+ALAGGVG+LLGW WL
Sbjct: 61  ISVGFVLVVLGLNRFKKSNRLQIDKYTNTIMENRVGLTEDYWPLYALAGGVGSLLGWAWL 120

Query: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLP 180
           FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAF+IGAGLQFLYV+SVIDRLP
Sbjct: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFSIGAGLQFLYVVSVIDRLP 180

Query: 181 FTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVV 240
           FTLLVLQKAV MVSGLPEVIRVAY FMIVMLLCMG+WSFGVAGIVASSMGDGGRWWL+VV
Sbjct: 181 FTLLVLQKAVKMVSGLPEVIRVAYAFMIVMLLCMGIWSFGVAGIVASSMGDGGRWWLLVV 240

Query: 241 FSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICY 300
           FSISLFW GAVFCNTLHVIVSGMVFLVL HG  ESSS+PSKSLV+ASRYAVTTSFGSICY
Sbjct: 241 FSISLFWAGAVFCNTLHVIVSGMVFLVLIHGGRESSSMPSKSLVKASRYAVTTSFGSICY 300

Query: 301 GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360
           GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS
Sbjct: 301 GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360

Query: 361 FNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMV 420
           FNRSARDAWELFQSTGVEALIAYDCSGAVLLMST+MGGL AGTCSGIWTW KWKDKVSMV
Sbjct: 361 FNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTIMGGLTAGTCSGIWTWIKWKDKVSMV 420

Query: 421 ACTATLMGMVMM------------------------------------SEMLHQRLQHRS 461
           ACTATLMGMV++                                    S+MLHQRLQHRS
Sbjct: 421 ACTATLMGMVLVGLAIVVVESAVTSIYICYAENPLLMQKWDAEFFNQISDMLHQRLQHRS 480

BLAST of Cp4.1LG09g02780 vs. ExPASy TrEMBL
Match: A0A6J1G1U8 (CTL-like protein DDB_G0274487 OS=Cucurbita moschata OX=3662 GN=LOC111449926 PE=3 SV=1)

HSP 1 Score: 852 bits (2201), Expect = 3.91e-310
Identity = 451/496 (90.93%), Postives = 454/496 (91.53%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60
           M DSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF
Sbjct: 1   MADSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGF 60

Query: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120
           VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS
Sbjct: 61  VLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGS 120

Query: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLV 180
           FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFA GAGLQFLYVISVIDRLPFTLLV
Sbjct: 121 FANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFASGAGLQFLYVISVIDRLPFTLLV 180

Query: 181 LQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240
           LQKAV MVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL
Sbjct: 181 LQKAVTMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISL 240

Query: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300
           FWVGAVFCNTLHVIVSGMVFLVLFHG GESSSLPSKSLVRASRYAVTTSFGSICYGSLFT
Sbjct: 241 FWVGAVFCNTLHVIVSGMVFLVLFHGGGESSSLPSKSLVRASRYAVTTSFGSICYGSLFT 300

Query: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360
           AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA
Sbjct: 301 AAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSA 360

Query: 361 RDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTAT 420
           RDAWELFQSTGVE L+AYDCSGAVLLMST+MGGL+AGTCSGIWTWFKWKDKVSMVACTAT
Sbjct: 361 RDAWELFQSTGVETLVAYDCSGAVLLMSTIMGGLIAGTCSGIWTWFKWKDKVSMVACTAT 420

Query: 421 LMGMVM------------------------------------MSEMLHQRLQHRSARARE 460
           LMGMVM                                    MSEMLHQRLQHRSARARE
Sbjct: 421 LMGMVMAGLAIVVVESAVTSIYICYAEDPLLIHRWDIEFFDQMSEMLHQRLQHRSARARE 480

BLAST of Cp4.1LG09g02780 vs. ExPASy TrEMBL
Match: A0A6J1HV06 (CTL-like protein DDB_G0274487 OS=Cucurbita maxima OX=3661 GN=LOC111467716 PE=3 SV=1)

HSP 1 Score: 845 bits (2183), Expect = 2.24e-307
Identity = 447/494 (90.49%), Postives = 451/494 (91.30%), Query Frame = 0

Query: 3   DSGSVSSSSLDSASVATADQVQNAARGRGQALSSESSRSWRDIFWSIVFIIHLIIVGFVL 62
           DSGSVSSSS DSASVATADQ+QNAARGRGQALSSES RSWRDIFWSIVFIIHLIIVGFVL
Sbjct: 4   DSGSVSSSSFDSASVATADQLQNAARGRGQALSSESPRSWRDIFWSIVFIIHLIIVGFVL 63

Query: 63  VILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFA 122
           VILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFA
Sbjct: 64  VILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFA 123

Query: 123 NHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQ 182
           NHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQ
Sbjct: 124 NHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQ 183

Query: 183 KAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFW 242
           KAV MVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFW
Sbjct: 184 KAVKMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFW 243

Query: 243 VGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFTAA 302
           VGAVFCNTLHVIVSGMVFLVLFHG GESSSLPSKSL  ASRYAVTTSFGSICYGSLFTAA
Sbjct: 244 VGAVFCNTLHVIVSGMVFLVLFHGGGESSSLPSKSLAWASRYAVTTSFGSICYGSLFTAA 303

Query: 303 IRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARD 362
           IRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARD
Sbjct: 304 IRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARD 363

Query: 363 AWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTATLM 422
           AWELFQSTGVEAL+AYDCSGAVLLMST+MGGL+AGTCSGIWTWFKWKDKVSMVACTATLM
Sbjct: 364 AWELFQSTGVEALVAYDCSGAVLLMSTVMGGLIAGTCSGIWTWFKWKDKVSMVACTATLM 423

Query: 423 GMVM------------------------------------MSEMLHQRLQHRSARAREVL 460
           GMVM                                    MSEMLHQRLQHRSARAREVL
Sbjct: 424 GMVMAGLAIVVVESAVTSIYICYAEDPLLIHRWDIEFFDQMSEMLHQRLQHRSARAREVL 483

BLAST of Cp4.1LG09g02780 vs. ExPASy TrEMBL
Match: A0A6J1GZB3 (CTL-like protein DDB_G0274487 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111458882 PE=3 SV=1)

HSP 1 Score: 799 bits (2064), Expect = 2.13e-289
Identity = 422/502 (84.06%), Postives = 439/502 (87.45%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGR-----GQALSSESSRSWRDIFWSIVFIIHL 60
           M DSGSVSSSS DSAS+AT DQVQNA RGR     GQALS ESSRSWRD+FWS VFIIHL
Sbjct: 1   MADSGSVSSSSFDSASIATVDQVQNAVRGRMSGVGGQALSDESSRSWRDVFWSFVFIIHL 60

Query: 61  IIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWL 120
           I VGFVLV+LGLNRFKKSNRLQIDK+TNTIMENRVGLTEDYWPL+ALAGGVG+LLGWTWL
Sbjct: 61  ISVGFVLVVLGLNRFKKSNRLQIDKYTNTIMENRVGLTEDYWPLYALAGGVGSLLGWTWL 120

Query: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLP 180
           FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAF+IGAGLQFLYVISVIDRLP
Sbjct: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFSIGAGLQFLYVISVIDRLP 180

Query: 181 FTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVV 240
           FTLLVLQKAV MVSGLPEVI+VAY FMIVMLLCMGVWSFGVAGIVASSMGDGGRWWL+VV
Sbjct: 181 FTLLVLQKAVKMVSGLPEVIKVAYAFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLLVV 240

Query: 241 FSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICY 300
           FSISLFW G VFCNTLHVIVSGMVFLVLFHG  ESSS+PSKSL++ASRYAVTTSFGSICY
Sbjct: 241 FSISLFWAGVVFCNTLHVIVSGMVFLVLFHGGRESSSMPSKSLMKASRYAVTTSFGSICY 300

Query: 301 GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360
           GSLFTA IRTLRWEIRGIRSKIG+NECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS
Sbjct: 301 GSLFTAPIRTLRWEIRGIRSKIGQNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360

Query: 361 FNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMV 420
           FNRSARDAWEL QSTGVEAL+AYDCSGAVLLMST+MGGL AGTCSGIWTW KWKDKVSMV
Sbjct: 361 FNRSARDAWELLQSTGVEALVAYDCSGAVLLMSTIMGGLTAGTCSGIWTWIKWKDKVSMV 420

Query: 421 ACTATLMGMVMM------------------------------------SEMLHQRLQHRS 461
           ACTATLMGMV++                                    SEMLHQRLQHRS
Sbjct: 421 ACTATLMGMVLVGLAIVVVESAVTSIYICYAEDPLLIQKWDAEFFNQISEMLHQRLQHRS 480

BLAST of Cp4.1LG09g02780 vs. ExPASy TrEMBL
Match: A0A0A0KR48 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608460 PE=3 SV=1)

HSP 1 Score: 795 bits (2052), Expect = 1.43e-287
Identity = 416/502 (82.87%), Postives = 437/502 (87.05%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGR-----GQALSSESSRSWRDIFWSIVFIIHL 60
           MGDSGSVSSSS DS SVATAD+  N  +GR     GQALS ESSRSWRDIFW  VF++HL
Sbjct: 1   MGDSGSVSSSSFDSVSVATADRAPNIVQGRMSGDGGQALSDESSRSWRDIFWCFVFMLHL 60

Query: 61  IIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWL 120
           I VGFVLV+LGLNRFKKSNRLQIDK+TNTIMENRVGLTEDYWPL+ALAGGVG+LLGWTWL
Sbjct: 61  ISVGFVLVVLGLNRFKKSNRLQIDKYTNTIMENRVGLTEDYWPLYALAGGVGSLLGWTWL 120

Query: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLP 180
           FLLGSFANH MKISVHILTTYLAVISVLCFWGQLFFWGV F+IGAGLQFLYVISVIDRLP
Sbjct: 121 FLLGSFANHAMKISVHILTTYLAVISVLCFWGQLFFWGVTFSIGAGLQFLYVISVIDRLP 180

Query: 181 FTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVV 240
           FTLLVLQKAV MVSGLPEVIRVAY FMIVMLLCMG+WSFGV+GIVASSMGDGGRWWL+VV
Sbjct: 181 FTLLVLQKAVKMVSGLPEVIRVAYVFMIVMLLCMGIWSFGVSGIVASSMGDGGRWWLLVV 240

Query: 241 FSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICY 300
           FSISLFW GAV CNTLHVIVSGMVFLVL HG  ESSS+PSKSL++ASRYAVTTSFGSICY
Sbjct: 241 FSISLFWAGAVLCNTLHVIVSGMVFLVLIHGGRESSSMPSKSLIKASRYAVTTSFGSICY 300

Query: 301 GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360
           GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS
Sbjct: 301 GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360

Query: 361 FNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMV 420
           FNRSARDAWELFQSTGVE L+AYDCSGAVLLMST+MGGL AGTCSGIWTW KWKDKVSMV
Sbjct: 361 FNRSARDAWELFQSTGVETLVAYDCSGAVLLMSTVMGGLTAGTCSGIWTWIKWKDKVSMV 420

Query: 421 ACTATLMGMVMM------------------------------------SEMLHQRLQHRS 461
           ACTATLMGMV++                                    SEMLHQRLQHRS
Sbjct: 421 ACTATLMGMVLVGLAIVVVESAVTSIYICYAENPLLIQKWDAEFFNQISEMLHQRLQHRS 480

BLAST of Cp4.1LG09g02780 vs. ExPASy TrEMBL
Match: A0A6J1II55 (CTL-like protein DDB_G0274487 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477654 PE=3 SV=1)

HSP 1 Score: 793 bits (2049), Expect = 4.09e-287
Identity = 420/502 (83.67%), Postives = 436/502 (86.85%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAARGR-----GQALSSESSRSWRDIFWSIVFIIHL 60
           M DSGS SSSS DSAS+AT DQVQNA RGR     GQALS ESSRSWRD+FWS  FIIHL
Sbjct: 1   MADSGSASSSSFDSASIATVDQVQNAVRGRMSGVGGQALSDESSRSWRDVFWSFAFIIHL 60

Query: 61  IIVGFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWL 120
           I VGFVLV+LGLNRFKKSNRLQIDK+TNTIMENRVGLTEDYWPL+ALAGGVG+LLGWTWL
Sbjct: 61  ISVGFVLVVLGLNRFKKSNRLQIDKYTNTIMENRVGLTEDYWPLYALAGGVGSLLGWTWL 120

Query: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLP 180
           FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAI AGLQFLYVISVIDRLP
Sbjct: 121 FLLGSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIVAGLQFLYVISVIDRLP 180

Query: 181 FTLLVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVV 240
           FTLLVLQKAV MVSGLPEVIRVAY FMIVMLLCMGVWSFGVAGIVASSMGDGGRWWL+VV
Sbjct: 181 FTLLVLQKAVKMVSGLPEVIRVAYAFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLLVV 240

Query: 241 FSISLFWVGAVFCNTLHVIVSGMVFLVLFHGDGESSSLPSKSLVRASRYAVTTSFGSICY 300
           FSISLFW G VFCNTLHVIVSGMVFLVLFHG  ESSS+PSKSL++ASRYAVTTSFGSICY
Sbjct: 241 FSISLFWAGVVFCNTLHVIVSGMVFLVLFHGGRESSSMPSKSLMKASRYAVTTSFGSICY 300

Query: 301 GSLFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360
           GSLFTA IRTLRWEIRGIRSKIG+NECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS
Sbjct: 301 GSLFTAPIRTLRWEIRGIRSKIGQNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKS 360

Query: 361 FNRSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMV 420
           FNRSARDAWEL QSTGVEAL+AYDCSGAVLLMST+MGGL AGTCSGIWTW KWKDKVSMV
Sbjct: 361 FNRSARDAWELLQSTGVEALVAYDCSGAVLLMSTIMGGLTAGTCSGIWTWIKWKDKVSMV 420

Query: 421 ACTATLMGMVMM------------------------------------SEMLHQRLQHRS 461
           ACTATLMGMV++                                    SEMLHQRLQHRS
Sbjct: 421 ACTATLMGMVLVGLAIVVVESAVTSIYICYAEDPLLIQKWDAEFFNQISEMLHQRLQHRS 480

BLAST of Cp4.1LG09g02780 vs. TAIR 10
Match: AT1G25500.2 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 608.6 bits (1568), Expect = 4.1e-174
Identity = 321/485 (66.19%), Postives = 371/485 (76.49%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAAR--GRGQALSSESSRSWRDIFWSIVFIIHLIIV 60
           M DS +  S S D AS+++ DQ    +R  G G       SR W D+FWS +F+IHLI +
Sbjct: 1   MSDSTNSVSPSSDYASISSGDQSLQRSRRSGDGNRAVVRESRHWHDVFWSAIFVIHLICL 60

Query: 61  GFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLL 120
           GFVL +LGLNRF+ S+RL ID++T   +EN  GLTEDYWPL+A+AGG+G  + W W  LL
Sbjct: 61  GFVLAVLGLNRFRISDRLNIDRYTQGFLENHKGLTEDYWPLYAVAGGIGVFISWVWSLLL 120

Query: 121 GSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTL 180
           GS+AN +MK+SVHILTTYLAV+SVLCFW +LFFWG AFA+G+ LQFLYVISVIDRLPFT+
Sbjct: 121 GSYANEMMKVSVHILTTYLAVVSVLCFWCRLFFWGGAFAVGSLLQFLYVISVIDRLPFTM 180

Query: 181 LVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSI 240
           LVL+KA+ +V GLP+VI VA+ F +VMLL M +WSFG AG+VASSMGD GRWWL+VV S+
Sbjct: 181 LVLRKALKLVWGLPKVIMVAHAFTVVMLLWMSLWSFGAAGVVASSMGDEGRWWLLVVLSV 240

Query: 241 SLFWVGAVFCNTLHVIVSGMVFLVLFH-GDGESSSLPSKSLVRASRYAVTTSFGSICYGS 300
           SLFW GAV CNT+HVIVSGMVF VLFH G  ESSSLP  SLV + RYAVTTSFGSICYGS
Sbjct: 241 SLFWTGAVLCNTVHVIVSGMVFHVLFHCGQEESSSLPPSSLVDSLRYAVTTSFGSICYGS 300

Query: 301 LFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFN 360
           LFTAAIRTLRWEIRG RSKI  NECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGK FN
Sbjct: 301 LFTAAIRTLRWEIRGFRSKICGNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKGFN 360

Query: 361 RSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVAC 420
           +SARDAWELFQSTGVEAL+AYDCSGAVLLM T+ GGL+ G+C GIW W K+ D+V MVA 
Sbjct: 361 KSARDAWELFQSTGVEALVAYDCSGAVLLMGTIFGGLITGSCIGIWAWIKYSDRVIMVAS 420

Query: 421 TATLMGMVM------------------------------------MSEMLHQRLQHRSAR 447
           TA LMGMV+                                    MSE+LH+RLQHRS+R
Sbjct: 421 TAMLMGMVLVGLGMVVVESAVTSIYICFAEDPLLIQRWDADFYTEMSEVLHRRLQHRSSR 480

BLAST of Cp4.1LG09g02780 vs. TAIR 10
Match: AT1G25500.1 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 595.5 bits (1534), Expect = 3.6e-170
Identity = 304/429 (70.86%), Postives = 351/429 (81.82%), Query Frame = 0

Query: 1   MGDSGSVSSSSLDSASVATADQVQNAAR--GRGQALSSESSRSWRDIFWSIVFIIHLIIV 60
           M DS +  S S D AS+++ DQ    +R  G G       SR W D+FWS +F+IHLI +
Sbjct: 1   MSDSTNSVSPSSDYASISSGDQSLQRSRRSGDGNRAVVRESRHWHDVFWSAIFVIHLICL 60

Query: 61  GFVLVILGLNRFKKSNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLL 120
           GFVL +LGLNRF+ S+RL ID++T   +EN  GLTEDYWPL+A+AGG+G  + W W  LL
Sbjct: 61  GFVLAVLGLNRFRISDRLNIDRYTQGFLENHKGLTEDYWPLYAVAGGIGVFISWVWSLLL 120

Query: 121 GSFANHVMKISVHILTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTL 180
           GS+AN +MK+SVHILTTYLAV+SVLCFW +LFFWG AFA+G+ LQFLYVISVIDRLPFT+
Sbjct: 121 GSYANEMMKVSVHILTTYLAVVSVLCFWCRLFFWGGAFAVGSLLQFLYVISVIDRLPFTM 180

Query: 181 LVLQKAVMMVSGLPEVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSI 240
           LVL+KA+ +V GLP+VI VA+ F +VMLL M +WSFG AG+VASSMGD GRWWL+VV S+
Sbjct: 181 LVLRKALKLVWGLPKVIMVAHAFTVVMLLWMSLWSFGAAGVVASSMGDEGRWWLLVVLSV 240

Query: 241 SLFWVGAVFCNTLHVIVSGMVFLVLFH-GDGESSSLPSKSLVRASRYAVTTSFGSICYGS 300
           SLFW GAV CNT+HVIVSGMVF VLFH G  ESSSLP  SLV + RYAVTTSFGSICYGS
Sbjct: 241 SLFWTGAVLCNTVHVIVSGMVFHVLFHCGQEESSSLPPSSLVDSLRYAVTTSFGSICYGS 300

Query: 301 LFTAAIRTLRWEIRGIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFN 360
           LFTAAIRTLRWEIRG RSKI  NECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGK FN
Sbjct: 301 LFTAAIRTLRWEIRGFRSKICGNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKGFN 360

Query: 361 RSARDAWELFQSTGVEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVAC 420
           +SARDAWELFQSTGVEAL+AYDCSGAVLLM T+ GGL+ G+C GIW W K+ D+V MVA 
Sbjct: 361 KSARDAWELFQSTGVEALVAYDCSGAVLLMGTIFGGLITGSCIGIWAWIKYSDRVIMVAS 420

Query: 421 TATLMGMVM 427
           TA LMGMV+
Sbjct: 421 TAMLMGMVL 429

BLAST of Cp4.1LG09g02780 vs. TAIR 10
Match: AT1G25500.3 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 553.1 bits (1424), Expect = 2.1e-157
Identity = 286/411 (69.59%), Postives = 325/411 (79.08%), Query Frame = 0

Query: 73  SNRLQIDKFTNTIMENRVGLTEDYWPLFALAGGVGTLLGWTWLFLLGSFANHVMKISVHI 132
           S+RL ID++T   +EN  GLTEDYWPL+A+AGG+G  + W W  LLGS+AN +MK+SVHI
Sbjct: 17  SDRLNIDRYTQGFLENHKGLTEDYWPLYAVAGGIGVFISWVWSLLLGSYANEMMKVSVHI 76

Query: 133 LTTYLAVISVLCFWGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQKAVMMVSGLP 192
           LTTYLAV+SVLCFW +LFFWG AFA+G+ LQFLYVISVIDRLPFT+LVL+KA+ +V GLP
Sbjct: 77  LTTYLAVVSVLCFWCRLFFWGGAFAVGSLLQFLYVISVIDRLPFTMLVLRKALKLVWGLP 136

Query: 193 EVIRVAYTFMIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFWVGAVFCNTLH 252
           +VI VA+ F +VMLL M +WSFG AG+VASSMGD GRWWL+VV S+SLFW GAV CNT+H
Sbjct: 137 KVIMVAHAFTVVMLLWMSLWSFGAAGVVASSMGDEGRWWLLVVLSVSLFWTGAVLCNTVH 196

Query: 253 VIVSGMVFLVLFH-GDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIR 312
           VIVSGMVF VLFH G  ESSSLP  SLV + RYAVTTSFGSICYGSLFTAAIRTLRWEIR
Sbjct: 197 VIVSGMVFHVLFHCGQEESSSLPPSSLVDSLRYAVTTSFGSICYGSLFTAAIRTLRWEIR 256

Query: 313 GIRSKIGKNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTG 372
           G RSKI  NECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGK FN+SARDAWELFQSTG
Sbjct: 257 GFRSKICGNECLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKGFNKSARDAWELFQSTG 316

Query: 373 VEALIAYDCSGAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTATLMGMVM----- 432
           VEAL+AYDCSGAVLLM T+ GGL+ G+C GIW W K+ D+V MVA TA LMGMV+     
Sbjct: 317 VEALVAYDCSGAVLLMGTIFGGLITGSCIGIWAWIKYSDRVIMVASTAMLMGMVLVGLGM 376

Query: 433 -------------------------------MSEMLHQRLQHRSARAREVL 447
                                          MSE+LH+RLQHRS+RAREVL
Sbjct: 377 VVVESAVTSIYICFAEDPLLIQRWDADFYTEMSEVLHRRLQHRSSRAREVL 427

BLAST of Cp4.1LG09g02780 vs. TAIR 10
Match: AT5G13760.1 (Plasma-membrane choline transporter family protein )

HSP 1 Score: 100.9 bits (250), Expect = 2.8e-21
Identity = 101/405 (24.94%), Postives = 183/405 (45.19%), Query Frame = 0

Query: 32  QALSSESSRSWRDIFWSIVFIIHLII-VGFV--LVILGLNRFKKSNRLQIDKFTNTIMEN 91
           Q L+S +S  + + F+ ++FI H ++ +GFV  LV  G+     SN         ++   
Sbjct: 129 QTLASLNSTKYTNKFFLLLFIFHKVVAIGFVGFLVFRGVQGLIGSN--------GSVKRK 188

Query: 92  RVGLTEDYWPLFALAGGVGTLLGWTWLF---LLGSFANHVMKISVHILTTYLAVISVLCF 151
              +     P    A  +  +L + W     +   F  H +  S  +++    ++ +LCF
Sbjct: 189 EKKILRFLLPQVEAASLLSIILAFLWQMAFRIWPDFMIHFILWSTFLMSLSSGIL-LLCF 248

Query: 152 ----WGQLFFWGVAFAIGAGLQFLYVISVIDRLPFTLLVLQKAVMMVSGLPEVIRVAYTF 211
                  +    +AF+IG G   LY   V  R+ F   +L K++  VS   ++    Y  
Sbjct: 249 QMPATDAVGVCLIAFSIGNG---LYACWVTRRIKFCSKILVKSLEPVSKFSDLNLPTYYM 308

Query: 212 MIVMLLCMGVWSFGVAGIVASSMGDGGRWWLIVVFSISLFWVGAVFCNTLHVIVSGMVFL 271
           +    L M +W FGV G +           +I+   +SL W   V  N +++ VS ++ L
Sbjct: 309 LAAGFLWMSMWIFGVIGALNFYFPP----LVIIGLVLSLAWTTEVMRNIVNLTVSRVIAL 368

Query: 272 VLFHGDGESSSLPSKSLVRASRYAVTTSFGSICYGSLFTAAIRTLRWEIRGIRSKIGKNE 331
               G   S+         + + A++ + GS C GSLF   I  LR   RG+    G++E
Sbjct: 369 YYLRGMQSSTRF-------SFQRALSRNLGSACLGSLFVPTIEALRILARGLNLLKGEDE 428

Query: 332 CLLCCVDFLFHLVETLVRFFNKYAYVQIAVYGKSFNRSARDAWELFQSTGVEALIAYDCS 391
            + CC +    L++ +    N +A+VQIA YGK F R+++D W+LF+   +  ++  D +
Sbjct: 429 FMFCCANCCLRLMDFIFEHGNGWAFVQIAAYGKGFVRASQDTWKLFEDEDMVEIVDADIT 488

Query: 392 GAVLLMSTMMGGLVAGTCSGIWTWFKWKDKVSMVACTATLMGMVM 427
            ++  ++ +  G V    +  WT   +K   + ++  A  +G +M
Sbjct: 489 SSICFLTGICSGCVCLIVAAAWTHTVYKPFTATISLLAFFIGYLM 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q869R12.0e-2428.85CTL-like protein DDB_G0274487 OS=Dictyostelium discoideum OX=44689 GN=DDB_G02744... [more]
P0CM931.1e-1423.59Protein PNS1 OS=Cryptococcus neoformans var. neoformans serotype D (strain B-350... [more]
P0CM921.1e-1423.59Protein PNS1 OS=Cryptococcus neoformans var. neoformans serotype D (strain JEC21... [more]
Q870V71.9e-1425.35Protein PNS1 OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 /... [more]
Q4I8E91.6e-1322.96Protein PNS1 OS=Gibberella zeae (strain ATCC MYA-4620 / CBS 123657 / FGSC 9075 /... [more]
Match NameE-valueIdentityDescription
XP_023542360.10.092.76CTL-like protein DDB_G0274487 [Cucurbita pepo subsp. pepo][more]
KAG6573984.17.04e-31090.74CTL-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7013042.1 ... [more]
XP_022945781.18.08e-31090.93CTL-like protein DDB_G0274487 [Cucurbita moschata][more]
XP_022968496.14.63e-30790.49CTL-like protein DDB_G0274487 [Cucurbita maxima][more]
XP_038891301.17.98e-29284.26CTL-like protein DDB_G0274487 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G1U83.91e-31090.93CTL-like protein DDB_G0274487 OS=Cucurbita moschata OX=3662 GN=LOC111449926 PE=3... [more]
A0A6J1HV062.24e-30790.49CTL-like protein DDB_G0274487 OS=Cucurbita maxima OX=3661 GN=LOC111467716 PE=3 S... [more]
A0A6J1GZB32.13e-28984.06CTL-like protein DDB_G0274487 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A0A0KR481.43e-28782.87Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G608460 PE=3 SV=1[more]
A0A6J1II554.09e-28783.67CTL-like protein DDB_G0274487 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147... [more]
Match NameE-valueIdentityDescription
AT1G25500.24.1e-17466.19Plasma-membrane choline transporter family protein [more]
AT1G25500.13.6e-17070.86Plasma-membrane choline transporter family protein [more]
AT1G25500.32.1e-15769.59Plasma-membrane choline transporter family protein [more]
AT5G13760.12.8e-2124.94Plasma-membrane choline transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007603Choline transporter-likePFAMPF04515Choline_transpocoord: 155..425
e-value: 4.6E-44
score: 151.0
IPR007603Choline transporter-likePANTHERPTHR12385CHOLINE TRANSPORTER-LIKE (SLC FAMILY 44)coord: 18..427
NoneNo IPR availablePANTHERPTHR12385:SF81PLASMA-MEMBRANE CHOLINE TRANSPORTER FAMILY PROTEINcoord: 18..427

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g02780.1Cp4.1LG09g02780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity