Cp4.1LG09g02480 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g02480 vs. ExPASy Swiss-Prot
Match: Q9C667 (Chaperonin 60 subunit beta 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B4 PE=1 SV=1)

HSP 1 Score: 787.3 bits (2032), Expect = 1.2e-226
Identity = 417/582 (71.65%), Postives = 493/582 (84.71%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MA S   +S L   +R  + KP+    S  P +    ++  KE++FNRDGS  KKLQ G 
Sbjct: 1   MAFSQAALSALPLSDRTFRKKPSSSSSS-SPNFVLRVRAAAKEVHFNRDGSVTKKLQAGA 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           D+VA+L+GVTLGPKGRNVVLQ+KYGPP+IVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTS++LA GLI EG+KVIS+G +P+Q+ARGIEKT  ALV ELK MSRE+ED
Sbjct: 121 TNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMSREIED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HE+AHVAAVSAGNDY VGNMIS+A +QVGR GVV IEKGK + N+L+IVEGMQF+ GYLS
Sbjct: 181 HELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFNRGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDR+K   EFHDCKLLLVDKKI++PK+MFKILDSAVKE++P++I+AE IEQ ALAP
Sbjct: 241 PYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQDALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKL+G LK AA+KAPAFGERKSH LDD+A  TGATVIR++ GL+LEK G EVLG+A 
Sbjct: 301 VIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEVLGTAK 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           +V++TKDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG IAI+QV
Sbjct: 361 RVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGIAIIQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GA TQVELKDKQL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N EQ IG
Sbjct: 421 GALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNTEQKIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFK+ALSYP RLIA+NA  NG++VI+KVL N +  YGYNAA + YEDLM AGI+DP+K
Sbjct: 481 AEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGIIDPTK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFP-RRTPLPIS 582
           VVRCCLEHA+SVA+TFLTSD VVV+IKE +P P    PLP S
Sbjct: 541 VVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 581

BLAST of Cp4.1LG09g02480 vs. ExPASy Swiss-Prot
Match: P08927 (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 732.6 bits (1890), Expect = 3.4e-210
Identity = 374/544 (68.75%), Postives = 464/544 (85.29%), Query Frame = 0

Query: 39  SMPKEIYFNRDGSALKKLQVGVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLK 98
           +M KE++FN+DGSA+KKLQ GV+ +A+LVGVTLGPKGRNVVL+SKYG PKIVNDG TV K
Sbjct: 48  AMAKELHFNKDGSAIKKLQNGVNKLADLVGVTLGPKGRNVVLESKYGSPKIVNDGVTVAK 107

Query: 99  EIELEDRLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIAR 158
           E+ELED +EN+G KLVRQA AKTNDLAGDG+TTSVVLAQGLIAEG+KV+++G +PV I R
Sbjct: 108 EVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGLIAEGVKVVAAGANPVLITR 167

Query: 159 GIEKTANALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEK 218
           GIEKT+ ALV+ELK MS+EVED E+A VAAVSAGN++ VGNMI++AL +VGRKGVV +E+
Sbjct: 168 GIEKTSKALVAELKKMSKEVEDSELADVAAVSAGNNHEVGNMIAEALSKVGRKGVVTLEE 227

Query: 219 GKSVDNSLQIVEGMQFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDS 278
           GKS +NSL +VEGMQFD GY+SPYFVTD +KM VEF +CKLLLVDKKI++ +++  IL+ 
Sbjct: 228 GKSAENSLYVVEGMQFDRGYISPYFVTDSEKMTVEFENCKLLLVDKKITNARDLINILED 287

Query: 279 AVKEKYPIVILAEGIEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGAT 338
           A++  +PIVI+AE IEQ ALA ++ NKLRG LK AA+KAP FGERKS YLDDIA LTG T
Sbjct: 288 AIRSGFPIVIIAEDIEQEALATLVVNKLRGSLKIAALKAPGFGERKSQYLDDIAILTGGT 347

Query: 339 VIREDSGLTLEKTGNEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEK 398
           VIRE+ GLTL+K   EVLG+A KVV+TKD+T IV DG+T+EAV KRV QI+  +E  E++
Sbjct: 348 VIREEVGLTLDKADKEVLGNAAKVVLTKDTTTIVGDGSTQEAVNKRVSQIKNQIEAAEQE 407

Query: 399 FPKKILNERIARLSGRIAILQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCL 458
           + K+ L+ERIA+LSG +A++QVGAQT+ ELK+K+LR+EDALNA+KAA+EEG+VVGGGC L
Sbjct: 408 YEKEKLSERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTL 467

Query: 459 LRLSTKVDAIKNVLENEEQMIGAEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNY 518
           LRL++KVDAIK+ L N+E+ +GA+I KRALSYP +LIA+NAGVNGSVV +KVL +++  Y
Sbjct: 468 LRLASKVDAIKDTLANDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSSDNPKY 527

Query: 519 GYNAATDHYEDLMKAGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPL 578
           GYNAAT  YEDLM AGI+DP+KVVRCCLEHA+SVAKTFL SD VVV+IKEPE  P   P+
Sbjct: 528 GYNAATGKYEDLMAAGIIDPTKVVRCCLEHASSVAKTFLMSDCVVVEIKEPESAPVGNPM 587

Query: 579 PISG 583
             SG
Sbjct: 588 DNSG 591

BLAST of Cp4.1LG09g02480 vs. ExPASy Swiss-Prot
Match: Q9LJE4 (Chaperonin 60 subunit beta 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B2 PE=1 SV=1)

HSP 1 Score: 729.6 bits (1882), Expect = 2.9e-209
Identity = 378/580 (65.17%), Postives = 478/580 (82.41%), Query Frame = 0

Query: 3   SSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGVDL 62
           SS T IS  SF  R +               +P+     KE++FN+DG+ ++KLQ GV+ 
Sbjct: 23  SSATSISSSSFGRRHNVCVRRS---------RPAIVCAAKELHFNKDGTTIRKLQTGVNK 82

Query: 63  VAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAKTN 122
           +A+LVGVTLGPKGRNVVL+SKYG P+IVNDG TV +E+ELED +EN+G KLVRQA AKTN
Sbjct: 83  LADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTN 142

Query: 123 DLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVEDHE 182
           DLAGDG+TTSVVLAQG IAEG+KV+++G +PV I RGIEKTA ALV+ELKLMS+EVED E
Sbjct: 143 DLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVNELKLMSKEVEDSE 202

Query: 183 IAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLSPY 242
           +A VAAVSAGN++ VG+MI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFD GY+SPY
Sbjct: 203 LADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPY 262

Query: 243 FVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAPVI 302
           FVTD +KM VE+ +CKLLLVDKK+++ +++  +L+ A++  YPI+I+AE IEQ ALA ++
Sbjct: 263 FVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLV 322

Query: 303 RNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSATKV 362
            NKLRG LK AA+KAP FGERKS YLDDIA LTGATVIRE+ GL+L+K G EVLG+A+KV
Sbjct: 323 VNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKV 382

Query: 363 VITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQVGA 422
           V+TK+ T IV DGTT+EAV KRV+QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGA
Sbjct: 383 VLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGA 442

Query: 423 QTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAE 482
           QT+ ELK+K+LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK+ LEN+E+ +GAE
Sbjct: 443 QTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLENDEEKVGAE 502

Query: 483 IFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVV 542
           I KRALSYP +LIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVV
Sbjct: 503 IVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYNAATGKYEDLMAAGIIDPTKVV 562

Query: 543 RCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 583
           RCCLEHAASVAKTFL SD VVV+I EPEP P   P+  SG
Sbjct: 563 RCCLEHAASVAKTFLMSDCVVVEIPEPEPVPAGNPMDNSG 593

BLAST of Cp4.1LG09g02480 vs. ExPASy Swiss-Prot
Match: P21241 (RuBisCO large subunit-binding protein subunit beta, chloroplastic OS=Brassica napus OX=3708 PE=2 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 4.6e-207
Identity = 364/539 (67.53%), Postives = 461/539 (85.53%), Query Frame = 0

Query: 35  PSPKSMPKEIYFNRDGSALKKLQVGVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGE 94
           P+     KE++FN+DG+ ++KLQ GV+ +A+LVGVTLGPKGRNVVL+SKYG P+IVNDG 
Sbjct: 50  PAVVCAAKELHFNKDGTTIRKLQAGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGV 109

Query: 95  TVLKEIELEDRLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPV 154
           TV +E+ELED +EN+G KLVRQA AKTNDLAGDG+TTSVVLAQG IAEG+KV+++G +PV
Sbjct: 110 TVAREVELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPV 169

Query: 155 QIARGIEKTANALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVV 214
            I RGIEKTA ALV+ELK MS+EVED E+A VAAVSAGN+  +G+MI++A+ +VGRKGVV
Sbjct: 170 LITRGIEKTAKALVAELKKMSKEVEDSELADVAAVSAGNNAEIGSMIAEAMSRVGRKGVV 229

Query: 215 QIEKGKSVDNSLQIVEGMQFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFK 274
            +E+GKS +N+L +VEGMQFD GY+SPYFVTD +KM VEF +CKLLLVDKKI++ +++  
Sbjct: 230 TLEEGKSAENALYVVEGMQFDRGYVSPYFVTDSEKMSVEFDNCKLLLVDKKITNARDLVG 289

Query: 275 ILDSAVKEKYPIVILAEGIEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATL 334
           +L+ A++  YPI+I+AE IEQ ALA ++ NKLRG LK AA+KAP FGERKS YLDDIA L
Sbjct: 290 VLEDAIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAIL 349

Query: 335 TGATVIREDSGLTLEKTGNEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLEN 394
           TGATVIRE+ GL+L+K G EVLG A KVV+TK+++ IV DG+T++AVQKRV QI+ L+E 
Sbjct: 350 TGATVIREEVGLSLDKAGKEVLGHAAKVVLTKETSTIVGDGSTQDAVQKRVTQIKNLIEQ 409

Query: 395 TEEKFPKKILNERIARLSGRIAILQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGG 454
            E+ + K+ LNERIA+LSG +A++QVGAQT+ ELK+K+LR+EDALNA+KAA+EEG+VVGG
Sbjct: 410 AEQDYEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGG 469

Query: 455 GCCLLRLSTKVDAIKNVLENEEQMIGAEIFKRALSYPTRLIARNAGVNGSVVIDKVLINN 514
           GC LLRL++KVDAIK  L+N+E+ +GA+I KRALSYP +LIA+NAGVNGSVV +KVL N+
Sbjct: 470 GCTLLRLASKVDAIKAALDNDEEKVGADIVKRALSYPLKLIAKNAGVNGSVVSEKVLSND 529

Query: 515 DMNYGYNAATDHYEDLMKAGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFP 574
           ++ +GYNAAT  YEDLM AGI+DP+KVVRCCLEHAASVAKTFL SD VVV+IKEPEP P
Sbjct: 530 NVKFGYNAATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIKEPEPVP 588

BLAST of Cp4.1LG09g02480 vs. ExPASy Swiss-Prot
Match: P21240 (Chaperonin 60 subunit beta 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CPN60B1 PE=1 SV=3)

HSP 1 Score: 720.3 bits (1858), Expect = 1.8e-206
Identity = 371/576 (64.41%), Postives = 478/576 (82.99%), Query Frame = 0

Query: 8   ISPLSFPNRRSK-PKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGVDLVAEL 67
           +S  SF  R+S  P+P +   ++            KE++FN+DG+ +++LQ GV+ +A+L
Sbjct: 30  LSSSSFGRRQSVCPRPRRSSSAI--------VCAAKELHFNKDGTTIRRLQAGVNKLADL 89

Query: 68  VGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAKTNDLAG 127
           VGVTLGPKGRNVVL+SKYG P+IVNDG TV +E+ELED +EN+G KLVRQA AKTNDLAG
Sbjct: 90  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 149

Query: 128 DGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVEDHEIAHV 187
           DG+TTSVVLAQG IAEG+KV+++G +PV I RGIEKTA ALV+ELK MS+EVED E+A V
Sbjct: 150 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADV 209

Query: 188 AAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLSPYFVTD 247
           AAVSAGN+  +GNMI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFD GY+SPYFVTD
Sbjct: 210 AAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTD 269

Query: 248 RQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAPVIRNKL 307
            +KM VEF +CKLLLVDKKI++ +++  +L+ A++  YPI+I+AE IEQ ALA ++ NKL
Sbjct: 270 SEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKL 329

Query: 308 RGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSATKVVITK 367
           RG LK AA++AP FGERKS YLDDIA LTGATVIRE+ GL+L+K G EVLG+A+KVV+TK
Sbjct: 330 RGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTK 389

Query: 368 DSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQVGAQTQV 427
           +++ IV DG+T++AV+KRV QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGAQT+ 
Sbjct: 390 ETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTET 449

Query: 428 ELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAEIFKR 487
           ELK+K+LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK  L+N+E+ +GA+I KR
Sbjct: 450 ELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKR 509

Query: 488 ALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCL 547
           ALSYP +LIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVVRCCL
Sbjct: 510 ALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCL 569

Query: 548 EHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 583
           EHAASVAKTFL SD VVV+IKEPEP P   P+  SG
Sbjct: 570 EHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNSG 597

BLAST of Cp4.1LG09g02480 vs. NCBI nr
Match: XP_023541180.1 (chaperonin 60 subunit beta 4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1120 bits (2896), Expect = 0.0
Identity = 590/590 (100.00%), Postives = 590/590 (100.00%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKRNGSLA 590
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKRNGSLA
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKRNGSLA 590

BLAST of Cp4.1LG09g02480 vs. NCBI nr
Match: KAG6574020.1 (Chaperonin 60 subunit beta 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1113 bits (2878), Expect = 0.0
Identity = 586/590 (99.32%), Postives = 588/590 (99.66%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDSGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVI+KDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVISKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKRNGSLA 590
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPEP PRRTPLPISGIKRNGSLA
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPVPRRTPLPISGIKRNGSLA 590

BLAST of Cp4.1LG09g02480 vs. NCBI nr
Match: XP_022945344.1 (chaperonin 60 subunit beta 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 581/587 (98.98%), Postives = 583/587 (99.32%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQ KYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQRKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDSGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMN+GYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNFGYNAATDHYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKRNG 587
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPE FPRRTPLPISGIKR G
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPESFPRRTPLPISGIKRMG 587

BLAST of Cp4.1LG09g02480 vs. NCBI nr
Match: XP_022968509.1 (chaperonin 60 subunit beta 4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 575/585 (98.29%), Postives = 580/585 (99.15%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MASSPTPISPLSFPNRRSKPKPNKPPPSLPP WKPSPKSMPKEIYFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPAWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSEL+LMSREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELELMSREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDSGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG EQAALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGFEQAALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFKRALSYPT+LIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKR 585
           VVRCCLEHAASVAKTFLTSDAVVVD+KEPEPFPRRT LP SGIKR
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDMKEPEPFPRRTTLPNSGIKR 585

BLAST of Cp4.1LG09g02480 vs. NCBI nr
Match: KAG7013080.1 (Chaperonin 60 subunit beta 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1073 bits (2774), Expect = 0.0
Identity = 582/636 (91.51%), Postives = 584/636 (91.82%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKP--NKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQV 60
           MASSPTPISPLSFPNRRSKPKP  NKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQV 60

Query: 61  -----------------------GVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGET 120
                                  GVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGET
Sbjct: 61  THSDEALDYLSSTTDLFGFVAQVGVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGET 120

Query: 121 VLKEIELEDRLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQ 180
           VLKEIELEDRLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQ
Sbjct: 121 VLKEIELEDRLENVGVKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQ 180

Query: 181 IARGIEKTANALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQ 240
           IARGIEKTANALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQ
Sbjct: 181 IARGIEKTANALVSELKLMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQ 240

Query: 241 IEKGKSVDNSLQIVEGMQFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKI 300
           IEKGKS+DNSLQIVEGMQFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKI
Sbjct: 241 IEKGKSIDNSLQIVEGMQFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKI 300

Query: 301 LDSAVKEKYPIVILAEGIEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLT 360
           LDSAVKEKYPIVILAEGIEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLT
Sbjct: 301 LDSAVKEKYPIVILAEGIEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLT 360

Query: 361 GATVIREDSGLTLEKTGNEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLE-- 420
           GATVIREDSGLTLEKTG EVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLE  
Sbjct: 361 GATVIREDSGLTLEKTGKEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLEVL 420

Query: 421 ----------------------NTEEKFPKKILNERIARLSGRIAILQVGAQTQVELKDK 480
                                 NTEEKFPKKILNERIARLSGRIAILQVGAQTQVELKDK
Sbjct: 421 ESSLIYAEISYISRDILSSILQNTEEKFPKKILNERIARLSGRIAILQVGAQTQVELKDK 480

Query: 481 QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAEIFKRALSYP 540
           QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAEIFKRALSYP
Sbjct: 481 QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAEIFKRALSYP 540

Query: 541 TRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCLEHAAS 587
           TRLIARNAGVNGSVVIDKVLINNDMN+GYNAATDHYEDLMKAGIMDPSKVVRCCLEHAAS
Sbjct: 541 TRLIARNAGVNGSVVIDKVLINNDMNFGYNAATDHYEDLMKAGIMDPSKVVRCCLEHAAS 600

BLAST of Cp4.1LG09g02480 vs. ExPASy TrEMBL
Match: A0A6J1G0R7 (chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449606 PE=3 SV=1)

HSP 1 Score: 1102 bits (2851), Expect = 0.0
Identity = 581/587 (98.98%), Postives = 583/587 (99.32%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQ KYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQRKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDSGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMN+GYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNFGYNAATDHYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKRNG 587
           VVRCCLEHAASVAKTFLTSDAVVVDIKEPE FPRRTPLPISGIKR G
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPESFPRRTPLPISGIKRMG 587

BLAST of Cp4.1LG09g02480 vs. ExPASy TrEMBL
Match: A0A6J1HZU9 (chaperonin 60 subunit beta 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111467722 PE=3 SV=1)

HSP 1 Score: 1093 bits (2826), Expect = 0.0
Identity = 575/585 (98.29%), Postives = 580/585 (99.15%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MASSPTPISPLSFPNRRSKPKPNKPPPSLPP WKPSPKSMPKEIYFNRDGSALKKLQVGV
Sbjct: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPAWKPSPKSMPKEIYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSEL+LMSREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELELMSREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKS+DNSLQIVEGMQFDSGYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSIDNSLQIVEGMQFDSGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEG EQAALAP
Sbjct: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGFEQAALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG
Sbjct: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFKRALSYPT+LIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK
Sbjct: 481 AEIFKRALSYPTKLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISGIKR 585
           VVRCCLEHAASVAKTFLTSDAVVVD+KEPEPFPRRT LP SGIKR
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVDMKEPEPFPRRTTLPNSGIKR 585

BLAST of Cp4.1LG09g02480 vs. ExPASy TrEMBL
Match: A0A6J1D5S9 (chaperonin 60 subunit beta 4, chloroplastic isoform X3 OS=Momordica charantia OX=3673 GN=LOC111017567 PE=3 SV=1)

HSP 1 Score: 1005 bits (2599), Expect = 0.0
Identity = 529/575 (92.00%), Postives = 553/575 (96.17%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           M SSPTPISPLS PN  S+PK N+PP SLPP W PSPKS+PKE+YFNRDGSALKKLQVGV
Sbjct: 1   MTSSPTPISPLSLPN--SRPKSNRPPSSLPPAWNPSPKSVPKELYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIE TA ALVSELKL+SREVED
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEMTARALVSELKLISREVED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFD GYLS
Sbjct: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDRGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDR+KM+ EFHDCKLLL+DKKISDPKEMFKILDSAVKEK+PIVILAEGIEQ ALAP
Sbjct: 241 PYFVTDRRKMMAEFHDCKLLLIDKKISDPKEMFKILDSAVKEKFPIVILAEGIEQEALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLGSAT
Sbjct: 301 VIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLGSAT 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           KVVI+KDSTLIVTDG TREAVQKRVLQIQKL+ENTEE FPKKILNERIARLSGRIAILQV
Sbjct: 361 KVVISKDSTLIVTDGRTREAVQKRVLQIQKLMENTEENFPKKILNERIARLSGRIAILQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GAQT+VELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVD IK+VLENEEQMIG
Sbjct: 421 GAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDDIKSVLENEEQMIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIF+RALSYPT+LIARNAGVNG++VIDKVLIN D+ YGYNAATD YEDLMKAGIMDPSK
Sbjct: 481 AEIFRRALSYPTKLIARNAGVNGNMVIDKVLINKDIKYGYNAATDCYEDLMKAGIMDPSK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRR 575
           VVRCCLEHAASVAKTFLTSDAVVV+IKEP+P PRR
Sbjct: 541 VVRCCLEHAASVAKTFLTSDAVVVEIKEPKPIPRR 573

BLAST of Cp4.1LG09g02480 vs. ExPASy TrEMBL
Match: A0A6J1D776 (chaperonin 60 subunit beta 4, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017567 PE=3 SV=1)

HSP 1 Score: 1000 bits (2585), Expect = 0.0
Identity = 529/578 (91.52%), Postives = 553/578 (95.67%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           M SSPTPISPLS PN  S+PK N+PP SLPP W PSPKS+PKE+YFNRDGSALKKLQVGV
Sbjct: 1   MTSSPTPISPLSLPN--SRPKSNRPPSSLPPAWNPSPKSVPKELYFNRDGSALKKLQVGV 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           DLVAELVGVTLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DLVAELVGVTLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREV-- 180
           TNDLAGDGSTTSVVLAQGLIAEGMKVISSGM+PVQIARGIE TA ALVSELKL+SREV  
Sbjct: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMNPVQIARGIEMTARALVSELKLISREVIK 180

Query: 181 -EDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSG 240
            EDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFD G
Sbjct: 181 VEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDRG 240

Query: 241 YLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAA 300
           YLSPYFVTDR+KM+ EFHDCKLLL+DKKISDPKEMFKILDSAVKEK+PIVILAEGIEQ A
Sbjct: 241 YLSPYFVTDRRKMMAEFHDCKLLLIDKKISDPKEMFKILDSAVKEKFPIVILAEGIEQEA 300

Query: 301 LAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLG 360
           LAPVIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTG EVLG
Sbjct: 301 LAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGKEVLG 360

Query: 361 SATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAI 420
           SATKVVI+KDSTLIVTDG TREAVQKRVLQIQKL+ENTEE FPKKILNERIARLSGRIAI
Sbjct: 361 SATKVVISKDSTLIVTDGRTREAVQKRVLQIQKLMENTEENFPKKILNERIARLSGRIAI 420

Query: 421 LQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQ 480
           LQVGAQT+VELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVD IK+VLENEEQ
Sbjct: 421 LQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDDIKSVLENEEQ 480

Query: 481 MIGAEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMD 540
           MIGAEIF+RALSYPT+LIARNAGVNG++VIDKVLIN D+ YGYNAATD YEDLMKAGIMD
Sbjct: 481 MIGAEIFRRALSYPTKLIARNAGVNGNMVIDKVLINKDIKYGYNAATDCYEDLMKAGIMD 540

Query: 541 PSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRR 575
           PSKVVRCCLEHAASVAKTFLTSDAVVV+IKEP+P PRR
Sbjct: 541 PSKVVRCCLEHAASVAKTFLTSDAVVVEIKEPKPIPRR 576

BLAST of Cp4.1LG09g02480 vs. ExPASy TrEMBL
Match: A0A5D3CCJ6 (RuBisCO large subunit-binding protein subunit beta OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00380 PE=3 SV=1)

HSP 1 Score: 995 bits (2572), Expect = 0.0
Identity = 524/592 (88.51%), Postives = 557/592 (94.09%), Query Frame = 0

Query: 1   MASSPTPISPLSFPN----RRSKPKP------NKPPPSLPPPWKPSPKSMPKEIYFNRDG 60
           MASSP+PISPLSF N     R+KPKP      NKPP S PP   P+PK  PKE+YFN DG
Sbjct: 1   MASSPSPISPLSFTNPISTSRTKPKPLPLPFPNKPPSSFPPASNPTPKPPPKELYFNHDG 60

Query: 61  SALKKLQVGVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVG 120
           S  KKLQ+GV+LVAELVG+TLGPKGRNVVLQ+KYGPPKIVNDGETVLKEIELED LENVG
Sbjct: 61  STFKKLQIGVNLVAELVGITLGPKGRNVVLQNKYGPPKIVNDGETVLKEIELEDPLENVG 120

Query: 121 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSE 180
           VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVI+SGM+PVQIARGIEKTA ALVSE
Sbjct: 121 VKLVRQAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVIASGMNPVQIARGIEKTAKALVSE 180

Query: 181 LKLMSREVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVE 240
           LKLMSREVEDHEIAHVAAVSAGNDYAVGN++SDALRQVG KGVVQIEKGKSVDNSLQIVE
Sbjct: 181 LKLMSREVEDHEIAHVAAVSAGNDYAVGNLVSDALRQVGWKGVVQIEKGKSVDNSLQIVE 240

Query: 241 GMQFDSGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 300
           GMQFD GYLSPYFVTDR+KM+VEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA
Sbjct: 241 GMQFDRGYLSPYFVTDRRKMVVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILA 300

Query: 301 EGIEQAALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEK 360
           EGIEQ ALAPVIRNKLRGVLKAAA+KAPAFGERKSHYLDDIATLTGATV+RED+GLTLEK
Sbjct: 301 EGIEQEALAPVIRNKLRGVLKAAAIKAPAFGERKSHYLDDIATLTGATVVREDNGLTLEK 360

Query: 361 TGNEVLGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIAR 420
           TG EVLGSA KVVI+KDSTLIVTDG TREAVQKRV+QIQKL+ENTEEKFPKKILNERIAR
Sbjct: 361 TGKEVLGSACKVVISKDSTLIVTDGNTREAVQKRVIQIQKLMENTEEKFPKKILNERIAR 420

Query: 421 LSGRIAILQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 480
           LSGRIAI+QVGAQT+VELKD+QLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN
Sbjct: 421 LSGRIAIIQVGAQTEVELKDRQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKN 480

Query: 481 VLENEEQMIGAEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDL 540
           VLEN+EQMIGAEIFKRALSYPT+LIARNAGVNGSVVIDK+L NND++YGYNAATD YE+L
Sbjct: 481 VLENDEQMIGAEIFKRALSYPTKLIARNAGVNGSVVIDKILSNNDLDYGYNAATDRYENL 540

Query: 541 MKAGIMDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 582
           MKAGIMDPSKVVRCCLEHAASV+KTFLTSDAVVVD+KE +  PRRTP+PISG
Sbjct: 541 MKAGIMDPSKVVRCCLEHAASVSKTFLTSDAVVVDMKESQLIPRRTPMPISG 592

BLAST of Cp4.1LG09g02480 vs. TAIR 10
Match: AT1G26230.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 787.3 bits (2032), Expect = 8.3e-228
Identity = 417/582 (71.65%), Postives = 493/582 (84.71%), Query Frame = 0

Query: 1   MASSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGV 60
           MA S   +S L   +R  + KP+    S  P +    ++  KE++FNRDGS  KKLQ G 
Sbjct: 1   MAFSQAALSALPLSDRTFRKKPSSSSSS-SPNFVLRVRAAAKEVHFNRDGSVTKKLQAGA 60

Query: 61  DLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAK 120
           D+VA+L+GVTLGPKGRNVVLQ+KYGPP+IVNDGETVLKEIELED LENVGVKLVRQAGAK
Sbjct: 61  DMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVRQAGAK 120

Query: 121 TNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVED 180
           TNDLAGDGSTTS++LA GLI EG+KVIS+G +P+Q+ARGIEKT  ALV ELK MSRE+ED
Sbjct: 121 TNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMSREIED 180

Query: 181 HEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLS 240
           HE+AHVAAVSAGNDY VGNMIS+A +QVGR GVV IEKGK + N+L+IVEGMQF+ GYLS
Sbjct: 181 HELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFNRGYLS 240

Query: 241 PYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAP 300
           PYFVTDR+K   EFHDCKLLLVDKKI++PK+MFKILDSAVKE++P++I+AE IEQ ALAP
Sbjct: 241 PYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQDALAP 300

Query: 301 VIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSAT 360
           VIRNKL+G LK AA+KAPAFGERKSH LDD+A  TGATVIR++ GL+LEK G EVLG+A 
Sbjct: 301 VIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEVLGTAK 360

Query: 361 KVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQV 420
           +V++TKDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG IAI+QV
Sbjct: 361 RVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGIAIIQV 420

Query: 421 GAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIG 480
           GA TQVELKDKQL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N EQ IG
Sbjct: 421 GALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNTEQKIG 480

Query: 481 AEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSK 540
           AEIFK+ALSYP RLIA+NA  NG++VI+KVL N +  YGYNAA + YEDLM AGI+DP+K
Sbjct: 481 AEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGIIDPTK 540

Query: 541 VVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFP-RRTPLPIS 582
           VVRCCLEHA+SVA+TFLTSD VVV+IKE +P P    PLP S
Sbjct: 541 VVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 581

BLAST of Cp4.1LG09g02480 vs. TAIR 10
Match: AT1G26230.2 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 766.9 bits (1979), Expect = 1.2e-221
Identity = 395/527 (74.95%), Postives = 464/527 (88.05%), Query Frame = 0

Query: 56  LQVGVDLVAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVR 115
           ++ G D+VA+L+GVTLGPKGRNVVLQ+KYGPP+IVNDGETVLKEIELED LENVGVKLVR
Sbjct: 3   VKAGADMVAKLLGVTLGPKGRNVVLQNKYGPPRIVNDGETVLKEIELEDPLENVGVKLVR 62

Query: 116 QAGAKTNDLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMS 175
           QAGAKTNDLAGDGSTTS++LA GLI EG+KVIS+G +P+Q+ARGIEKT  ALV ELK MS
Sbjct: 63  QAGAKTNDLAGDGSTTSIILAHGLITEGIKVISAGTNPIQVARGIEKTTKALVLELKSMS 122

Query: 176 REVEDHEIAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFD 235
           RE+EDHE+AHVAAVSAGNDY VGNMIS+A +QVGR GVV IEKGK + N+L+IVEGMQF+
Sbjct: 123 REIEDHELAHVAAVSAGNDYEVGNMISNAFQQVGRTGVVTIEKGKYLVNNLEIVEGMQFN 182

Query: 236 SGYLSPYFVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQ 295
            GYLSPYFVTDR+K   EFHDCKLLLVDKKI++PK+MFKILDSAVKE++P++I+AE IEQ
Sbjct: 183 RGYLSPYFVTDRRKREAEFHDCKLLLVDKKITNPKDMFKILDSAVKEEFPVLIVAEDIEQ 242

Query: 296 AALAPVIRNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEV 355
            ALAPVIRNKL+G LK AA+KAPAFGERKSH LDD+A  TGATVIR++ GL+LEK G EV
Sbjct: 243 DALAPVIRNKLKGNLKVAAIKAPAFGERKSHCLDDLAIFTGATVIRDEMGLSLEKAGKEV 302

Query: 356 LGSATKVVITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRI 415
           LG+A +V++TKDSTLIVT+G T++AV +RV QI+ L+ENTEE F KKILNER+ARLSG I
Sbjct: 303 LGTAKRVLVTKDSTLIVTNGFTQKAVDERVSQIKNLIENTEENFQKKILNERVARLSGGI 362

Query: 416 AILQVGAQTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENE 475
           AI+QVGA TQVELKDKQL++EDALNA+K+AIEEG+VVGGGC LLRL+TKVD IK  L+N 
Sbjct: 363 AIIQVGALTQVELKDKQLKVEDALNATKSAIEEGIVVGGGCALLRLATKVDRIKETLDNT 422

Query: 476 EQMIGAEIFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGI 535
           EQ IGAEIFK+ALSYP RLIA+NA  NG++VI+KVL N +  YGYNAA + YEDLM AGI
Sbjct: 423 EQKIGAEIFKKALSYPIRLIAKNADTNGNIVIEKVLSNKNTMYGYNAAKNQYEDLMLAGI 482

Query: 536 MDPSKVVRCCLEHAASVAKTFLTSDAVVVDIKEPEPFP-RRTPLPIS 582
           +DP+KVVRCCLEHA+SVA+TFLTSD VVV+IKE +P P    PLP S
Sbjct: 483 IDPTKVVRCCLEHASSVAQTFLTSDCVVVEIKEIKPRPIINPPLPTS 529

BLAST of Cp4.1LG09g02480 vs. TAIR 10
Match: AT3G13470.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 729.6 bits (1882), Expect = 2.1e-210
Identity = 378/580 (65.17%), Postives = 478/580 (82.41%), Query Frame = 0

Query: 3   SSPTPISPLSFPNRRSKPKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGVDL 62
           SS T IS  SF  R +               +P+     KE++FN+DG+ ++KLQ GV+ 
Sbjct: 23  SSATSISSSSFGRRHNVCVRRS---------RPAIVCAAKELHFNKDGTTIRKLQTGVNK 82

Query: 63  VAELVGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAKTN 122
           +A+LVGVTLGPKGRNVVL+SKYG P+IVNDG TV +E+ELED +EN+G KLVRQA AKTN
Sbjct: 83  LADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTN 142

Query: 123 DLAGDGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVEDHE 182
           DLAGDG+TTSVVLAQG IAEG+KV+++G +PV I RGIEKTA ALV+ELKLMS+EVED E
Sbjct: 143 DLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVNELKLMSKEVEDSE 202

Query: 183 IAHVAAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLSPY 242
           +A VAAVSAGN++ VG+MI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFD GY+SPY
Sbjct: 203 LADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPY 262

Query: 243 FVTDRQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAPVI 302
           FVTD +KM VE+ +CKLLLVDKK+++ +++  +L+ A++  YPI+I+AE IEQ ALA ++
Sbjct: 263 FVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLV 322

Query: 303 RNKLRGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSATKV 362
            NKLRG LK AA+KAP FGERKS YLDDIA LTGATVIRE+ GL+L+K G EVLG+A+KV
Sbjct: 323 VNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKV 382

Query: 363 VITKDSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQVGA 422
           V+TK+ T IV DGTT+EAV KRV+QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGA
Sbjct: 383 VLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGA 442

Query: 423 QTQVELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAE 482
           QT+ ELK+K+LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK+ LEN+E+ +GAE
Sbjct: 443 QTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKDTLENDEEKVGAE 502

Query: 483 IFKRALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVV 542
           I KRALSYP +LIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVV
Sbjct: 503 IVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKFGYNAATGKYEDLMAAGIIDPTKVV 562

Query: 543 RCCLEHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 583
           RCCLEHAASVAKTFL SD VVV+I EPEP P   P+  SG
Sbjct: 563 RCCLEHAASVAKTFLMSDCVVVEIPEPEPVPAGNPMDNSG 593

BLAST of Cp4.1LG09g02480 vs. TAIR 10
Match: AT1G55490.1 (chaperonin 60 beta )

HSP 1 Score: 720.3 bits (1858), Expect = 1.3e-207
Identity = 371/576 (64.41%), Postives = 478/576 (82.99%), Query Frame = 0

Query: 8   ISPLSFPNRRSK-PKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGVDLVAEL 67
           +S  SF  R+S  P+P +   ++            KE++FN+DG+ +++LQ GV+ +A+L
Sbjct: 30  LSSSSFGRRQSVCPRPRRSSSAI--------VCAAKELHFNKDGTTIRRLQAGVNKLADL 89

Query: 68  VGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAKTNDLAG 127
           VGVTLGPKGRNVVL+SKYG P+IVNDG TV +E+ELED +EN+G KLVRQA AKTNDLAG
Sbjct: 90  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 149

Query: 128 DGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVEDHEIAHV 187
           DG+TTSVVLAQG IAEG+KV+++G +PV I RGIEKTA ALV+ELK MS+EVED E+A V
Sbjct: 150 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADV 209

Query: 188 AAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLSPYFVTD 247
           AAVSAGN+  +GNMI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFD GY+SPYFVTD
Sbjct: 210 AAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTD 269

Query: 248 RQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAPVIRNKL 307
            +KM VEF +CKLLLVDKKI++ +++  +L+ A++  YPI+I+AE IEQ ALA ++ NKL
Sbjct: 270 SEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKL 329

Query: 308 RGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSATKVVITK 367
           RG LK AA++AP FGERKS YLDDIA LTGATVIRE+ GL+L+K G EVLG+A+KVV+TK
Sbjct: 330 RGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTK 389

Query: 368 DSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQVGAQTQV 427
           +++ IV DG+T++AV+KRV QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGAQT+ 
Sbjct: 390 ETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTET 449

Query: 428 ELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAEIFKR 487
           ELK+K+LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK  L+N+E+ +GA+I KR
Sbjct: 450 ELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKR 509

Query: 488 ALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCL 547
           ALSYP +LIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVVRCCL
Sbjct: 510 ALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCL 569

Query: 548 EHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 583
           EHAASVAKTFL SD VVV+IKEPEP P   P+  SG
Sbjct: 570 EHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNSG 597

BLAST of Cp4.1LG09g02480 vs. TAIR 10
Match: AT1G55490.2 (chaperonin 60 beta )

HSP 1 Score: 720.3 bits (1858), Expect = 1.3e-207
Identity = 371/576 (64.41%), Postives = 478/576 (82.99%), Query Frame = 0

Query: 8   ISPLSFPNRRSK-PKPNKPPPSLPPPWKPSPKSMPKEIYFNRDGSALKKLQVGVDLVAEL 67
           +S  SF  R+S  P+P +   ++            KE++FN+DG+ +++LQ GV+ +A+L
Sbjct: 30  LSSSSFGRRQSVCPRPRRSSSAI--------VCAAKELHFNKDGTTIRRLQAGVNKLADL 89

Query: 68  VGVTLGPKGRNVVLQSKYGPPKIVNDGETVLKEIELEDRLENVGVKLVRQAGAKTNDLAG 127
           VGVTLGPKGRNVVL+SKYG P+IVNDG TV +E+ELED +EN+G KLVRQA AKTNDLAG
Sbjct: 90  VGVTLGPKGRNVVLESKYGSPRIVNDGVTVAREVELEDPVENIGAKLVRQAAAKTNDLAG 149

Query: 128 DGSTTSVVLAQGLIAEGMKVISSGMSPVQIARGIEKTANALVSELKLMSREVEDHEIAHV 187
           DG+TTSVVLAQG IAEG+KV+++G +PV I RGIEKTA ALV+ELK MS+EVED E+A V
Sbjct: 150 DGTTTSVVLAQGFIAEGVKVVAAGANPVLITRGIEKTAKALVTELKKMSKEVEDSELADV 209

Query: 188 AAVSAGNDYAVGNMISDALRQVGRKGVVQIEKGKSVDNSLQIVEGMQFDSGYLSPYFVTD 247
           AAVSAGN+  +GNMI++A+ +VGRKGVV +E+GKS +N+L +VEGMQFD GY+SPYFVTD
Sbjct: 210 AAVSAGNNDEIGNMIAEAMSKVGRKGVVTLEEGKSAENNLYVVEGMQFDRGYISPYFVTD 269

Query: 248 RQKMIVEFHDCKLLLVDKKISDPKEMFKILDSAVKEKYPIVILAEGIEQAALAPVIRNKL 307
            +KM VEF +CKLLLVDKKI++ +++  +L+ A++  YPI+I+AE IEQ ALA ++ NKL
Sbjct: 270 SEKMSVEFDNCKLLLVDKKITNARDLVGVLEDAIRGGYPILIIAEDIEQEALATLVVNKL 329

Query: 308 RGVLKAAAVKAPAFGERKSHYLDDIATLTGATVIREDSGLTLEKTGNEVLGSATKVVITK 367
           RG LK AA++AP FGERKS YLDDIA LTGATVIRE+ GL+L+K G EVLG+A+KVV+TK
Sbjct: 330 RGTLKIAALRAPGFGERKSQYLDDIAILTGATVIREEVGLSLDKAGKEVLGNASKVVLTK 389

Query: 368 DSTLIVTDGTTREAVQKRVLQIQKLLENTEEKFPKKILNERIARLSGRIAILQVGAQTQV 427
           +++ IV DG+T++AV+KRV QI+ L+E  E+ + K+ LNERIA+LSG +A++QVGAQT+ 
Sbjct: 390 ETSTIVGDGSTQDAVKKRVTQIKNLIEQAEQDYEKEKLNERIAKLSGGVAVIQVGAQTET 449

Query: 428 ELKDKQLRIEDALNASKAAIEEGVVVGGGCCLLRLSTKVDAIKNVLENEEQMIGAEIFKR 487
           ELK+K+LR+EDALNA+KAA+EEG+VVGGGC LLRL++KVDAIK  L+N+E+ +GA+I KR
Sbjct: 450 ELKEKKLRVEDALNATKAAVEEGIVVGGGCTLLRLASKVDAIKATLDNDEEKVGADIVKR 509

Query: 488 ALSYPTRLIARNAGVNGSVVIDKVLINNDMNYGYNAATDHYEDLMKAGIMDPSKVVRCCL 547
           ALSYP +LIA+NAGVNGSVV +KVL N+++ +GYNAAT  YEDLM AGI+DP+KVVRCCL
Sbjct: 510 ALSYPLKLIAKNAGVNGSVVSEKVLSNDNVKFGYNAATGKYEDLMAAGIIDPTKVVRCCL 569

Query: 548 EHAASVAKTFLTSDAVVVDIKEPEPFPRRTPLPISG 583
           EHAASVAKTFL SD VVV+IKEPEP P   P+  SG
Sbjct: 570 EHAASVAKTFLMSDCVVVEIKEPEPVPVGNPMDNSG 597