Cp4.1LG09g00380 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g00380
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionSpermidine synthase
LocationCp4.1LG09: 211486 .. 215769 (+)
RNA-Seq ExpressionCp4.1LG09g00380
SyntenyCp4.1LG09g00380
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATATGGAAGAGTGTGCAGAGAGCAGTGCAACAGAGTGGAACGAAAGAAAAAGAAGACGAGGAGAGTGAGAAGAGAAAAAGAGAAAAAGAGGAGAACTTCCATTTACAATTCACGCTCCTTCCTTCCCTCATCGGAAGTTGTAAAACCCAATTTTGATTTTGTAATAATATTCTTGTTCTTCGTCAATTTTAGTGTTCATTTTTCATCTGCTACTCCAACCAGTCCCACTGCGTCTTCATCTATATAAACCCAACCTTTCCGCCCTTCTATCCTCTGCCACTCACGCATACACTGCCTGCAATTTCTCACCACCGGCTTTTGGGCTCTCTGATTCTCTCCTCCCGCTCGCTTCGCCATTCTTCGACTCTTCCGTGTAATCTTTCCTTCCCTTTTCTCTTTCCTTTTCAATCTCTTCTTCTTTCTCTGCTCTCTACTTTTGTTTCTGAAATGGGGTATTCGAAACCTTGATCACTGATGTTTGGGTTTTGGGTTTTGGGTTTTGGGTTTTATTCGTTTTCCAGTTTCCATCACAAAGATCTCCACCTGCTCTGCTTTTCTCTGTTCACTCTAATACTCGTGTCATTTCCTAACCCTGCAGATTTTAAGGAAAAAACAAAAACAAAATTTGGATGTTTGAACACCTTTTAGAGCTAAAATTAAGCATGAAACTATTGAAGGAAGGACGGAAGGAAGTATTTGGATTTCGCGTACTTTATTGGTCGGTGTGTGTAAGATAGTGATACACGCTTTCTTATTTTGTCCCTTTATAGTTTGTGGTCTTTGTGTTCCATCTTTATTTTCTTGTTGAACCGACTCATAAACAATGGTTCATGCTCGGTCTGCTGCGGAAACTGAAAGAACGTGGGGAATCTGAACTTCTTTTTCATTTTAAAAATAAACTTCTCTCATCTTTTCGGACCTTAAAATCCAGTTGAGCTACCTGAATTAATAGAAGGTTCATCATGAACAGTCTGGCTTATGGACATAGTTCTACTCTGTTTGTACCATTATAATATATCCTTGATTCCACTTCTGAGGTTTACCATGACTTTCATCAGTTCTTTTTTTATACCTATTCTCCATAGGGGTCTGAAGCTCTCTATTCCGATGGATTTCAGTCATATCATTTGCTTATCTTTCTTGACCTGGCTTTGTGAAACCTTATCCTTTGTTCTTGTCACCTTACATGATCTTATAGTAAATTTCAAACCTGGTAGGAACTCTAAAAGTTAACGAGAGAGATCATTTATTTTCTCTATCTCCAGATTTAACATAATTCTGATGGCATTCTTTTTTTCAGTTTTTTTTTTTTTTTTTCCTCTGATCAAAAGCTTTGCATTGTACACTGGAAAAAGCTGCGTGACTGTGCGTATTGGGTTGTGATCTAAGTTACTGTAAATCCACTTCCTATACAACTTGCTGATGCTTATTTGTTTCTGCTGATGATTATTCCTCGATCTTGTTTTGATGGGATTTCTTGTTATACATTTTGTCTTTTCTTGATGGTAACAAAGCATATTAGTGGGTGACATGTATTTGTCCAAAAGCCAAAAAACTTATATAGTCATAACGAAGCTATAGGTTCGATAATTTCATTTGTCCACTGGTGACTTCAATTTCTTCCTTGGAAATCTTGAATCTGCAGAAAGTAAAGATGCAAATGCTTACAAAATGTATAATCTATTACTTTCCCACGACTTATGTATGGTAGAAGAGCACGAGTCACTCAAAAGATGTCTCTTGTATACATATCTTCTTTCTCCATGTTTATGATGATGTGCTATTCTTGATGTTCCAGAGGCTGGAGACTGAACAAAAACGATAATACATGGAGGACGTCACAGGAAGAAGTTTGGAATGCCAGACAATTATGGATGGGAAGGCGACTAACGGGAATGGCTTAGATAAGCCCATTCCGTCTTGTTGTTTGAAGGCTAAGGCTTCAGATCCTGAGCTTGAGGCTCAATGCCATTCAACTGTTGTTTCTGGCTGGATTTCAGAATTTCAGTCTGGCTCTGGTCATTCTCCTTTTCTTTAATCTCCTTGTGAAGCTCTTGGTTCTGTTTTGGAAACCGTGGCTCACCATGTGGATATTAACATCAGTTGCGCTCGTTTCCTTTGCAGATAAAGCTAGCAAGTTCGTTTATTTCAACAACCCAATGTGGCCGGGTGAGTGCTTTTCTTGTATTTCTTGTTAATGGTCTCTTTTTCATGCTGAGGGACTTCATTTTACTTTCATTCTTGACAAGGAGTTGAATGTTCATATAAACGTTCACCACTTTCTGCAACATCAAAACTTTTTGACGTATATTTGATTGCACTAGTGTTACATAAGCCACGCGTCAAATGTTTGCATTTGACAAGCTGATGTTATTGTCAATCTTTCAGGAGAAGCACATTCTCTGCAAGTAGAGAAGATACTATTTAAAGGAAAGTCAGAGTACCAAGAGGTCATGGTTTTTGAGGTACCATTCGCTGAGACAATGTTTTTACCTTCGCAATTCATTTGCTTGGTTTCTTTATTAATACTTTACTTGTTTGTCTCTATTTGTTTACAGTCTGCATCATATGGAAAAGTCCTTGTTCTTGATGGCATTGTTCAACTGACAGAGAAAGATGAATGTGCCTACCAGGAAATGATAGCGCATCTTCCTCTTTGTTCGATCCCGTCTCCTAAAACCGTTAGTCCTCCCAAAAATTTGATTCTGTGCCAATTCCATCTCTTATTAAGAAATTTTATTTTTCTCTTCCAACTTTTGCATAATTTTAAGACATAAAATGACATTATTTAGGTTCTGGTTGTTGGCGGAGGTGATGGCGGAGTCCTGAGGGAAATTTCTCGTCACGACTCAGTGGAGCACATCGACATTTGTGAGATAGATGAGATGGTCATTAATGTAAGTGTTCATTAGAGACATGTTGCAAATTGTATGATAAGTTCATAATGAAACAACTTGCTGCTGTGGTATGTTTCAAGATTGTGACCTAATGCTTCATTTCCCTGTTTTCTTAATTCCTAGGTGTCAAAACAATTTTTCCCCCAACTAGCTGTTGGATTCGAGGACCCTCGTGTTCACCTTCATGTTGGTGATGGTGCGTCGAAAGTCGAGACATCTTATTCCTTTTTTTGTTTCTTATGCTCTTTCCTATATAATTTGCATCTTTGTTATATCAGCTGTTGAATTTCTTCGGAATGCAAAGCAAGGGAAGTATGATGCCGTCATTGTTGATTCCTCTGATCCTGTGGGTAAGTTAATGATTTGTAACAGAGAAGAATAAAAAAAAAAAAAAATGGTGCAACTCTGCAAGCTACATATCTTTTCGTTCAACAATTAGAACCTTAAATTTATGTTCTCTTTCGAAAGACTTGTGCTCGTAAACTCATCTTCTAACCGGGGGTTCTTGTAATAAAGGTCCTGCCCAAGAACTGGTGGAGATGCCATTTTTTCAGATGATAGCCCGAGCATTGCGACCTGGTGGTGTTCTCTGTAACATGGCTGAAAGTATGTGGCTGCATACACATCTCATTCAGGACATGATATCAATATGTCGCCAAACATTCAAGGATGTTCACTATGCATGGACCAGTGTTCCAACATATCCAAGTGGTGTTATAGGGTTTCTACTCTGCTCAACTGAAGGGCCAACAGTTGATTTTAGGAACCCTGTGAATCCCATCGAGAAGTTAGGACATCTCAAGTCAAAAAGAGAACTCAAATTCTACAACTCTGAGGTAACCACACAAGAGCTCTTTAACTTAACTAAGATTCGATACAATGTTGATGTAGAGATACATCCATCGACACACTATATGGATTAAAACTTCGGTTGATACTAATAATGGATGACATTCCAACAGATGCACTTGGCTGCATTTGCGCTGCCATCGTTTCTCAAGAGCGAGGTGAAGGCACTTAGTGATGCTTCAGCTTCATTTGAAAAGGGAAGCATTTCATCCTAAGTAGGTGACTGTTTGAAATGCTTCTTCCAAGGTGGTGATGGGCACTAGTGTTGAGTGTATACATAAAATTATTGGAGTGGCTGAAACATCTCTACTGTGTGCTGAGGGAGGGCATCGTTGATGATATTATTTAACAAGTTCAGGTTTGTTATCCTAGTTAGAAGAAAAGTGTTTAGCTGTTTTTGTTGTTTTTCCATGGCTGATTGGTATGCCAATTAGCCGATAAATAAAGAAAACCAAGAAAGAAACCACTTCCTAATAATTAATAAAAATGGGGAATTTATATAACAATGATTCCAGGTCTTGGTTCTACTAATAATCT

mRNA sequence

TATATGGAAGAGTGTGCAGAGAGCAGTGCAACAGAGTGGAACGAAAGAAAAAGAAGACGAGGAGAGTGAGAAGAGAAAAAGAGAAAAAGAGGAGAACTTCCATTTACAATTCACGCTCCTTCCTTCCCTCATCGGAAGTTGTAAAACCCAATTTTGATTTTGTAATAATATTCTTGTTCTTCGTCAATTTTAGTGTTCATTTTTCATCTGCTACTCCAACCAGTCCCACTGCGTCTTCATCTATATAAACCCAACCTTTCCGCCCTTCTATCCTCTGCCACTCACGCATACACTGCCTGCAATTTCTCACCACCGGCTTTTGGGCTCTCTGATTCTCTCCTCCCGCTCGCTTCGCCATTCTTCGACTCTTCCGTAGGCTGGAGACTGAACAAAAACGATAATACATGGAGGACGTCACAGGAAGAAGTTTGGAATGCCAGACAATTATGGATGGGAAGGCGACTAACGGGAATGGCTTAGATAAGCCCATTCCGTCTTGTTGTTTGAAGGCTAAGGCTTCAGATCCTGAGCTTGAGGCTCAATGCCATTCAACTGTTGTTTCTGGCTGGATTTCAGAATTTCAGTCTGGCTCTGATAAAGCTAGCAAGTTCGTTTATTTCAACAACCCAATGTGGCCGGGAGAAGCACATTCTCTGCAAGTAGAGAAGATACTATTTAAAGGAAAGTCAGAGTACCAAGAGGTCATGGTTTTTGAGTCTGCATCATATGGAAAAGTCCTTGTTCTTGATGGCATTGTTCAACTGACAGAGAAAGATGAATGTGCCTACCAGGAAATGATAGCGCATCTTCCTCTTTGTTCGATCCCGTCTCCTAAAACCGTTCTGGTTGTTGGCGGAGGTGATGGCGGAGTCCTGAGGGAAATTTCTCGTCACGACTCAGTGGAGCACATCGACATTTGTGAGATAGATGAGATGGTCATTAATGTGTCAAAACAATTTTTCCCCCAACTAGCTGTTGGATTCGAGGACCCTCGTGTTCACCTTCATGTTGGTGATGCTGTTGAATTTCTTCGGAATGCAAAGCAAGGGAAGTATGATGCCGTCATTGTTGATTCCTCTGATCCTGTGGGTCCTGCCCAAGAACTGGTGGAGATGCCATTTTTTCAGATGATAGCCCGAGCATTGCGACCTGGTGGTGTTCTCTGTAACATGGCTGAAAGTATGTGGCTGCATACACATCTCATTCAGGACATGATATCAATATGTCGCCAAACATTCAAGGATGTTCACTATGCATGGACCAGTGTTCCAACATATCCAAGTGGTGTTATAGGGTTTCTACTCTGCTCAACTGAAGGGCCAACAGTTGATTTTAGGAACCCTGTGAATCCCATCGAGAAGTTAGGACATCTCAAGTCAAAAAGAGAACTCAAATTCTACAACTCTGAGATGCACTTGGCTGCATTTGCGCTGCCATCGTTTCTCAAGAGCGAGGTGAAGGCACTTAGTGATGCTTCAGCTTCATTTGAAAAGGGAAGCATTTCATCCTAAGTAGGTGACTGTTTGAAATGCTTCTTCCAAGGTGGTGATGGGCACTAGTGTTGAGTGTATACATAAAATTATTGGAGTGGCTGAAACATCTCTACTGTGTGCTGAGGGAGGGCATCGTTGATGATATTATTTAACAAGTTCAGGTTTGTTATCCTAGTTAGAAGAAAAGTGTTTAGCTGTTTTTGTTGTTTTTCCATGGCTGATTGGTATGCCAATTAGCCGATAAATAAAGAAAACCAAGAAAGAAACCACTTCCTAATAATTAATAAAAATGGGGAATTTATATAACAATGATTCCAGGTCTTGGTTCTACTAATAATCT

Coding sequence (CDS)

ATGGAGGACGTCACAGGAAGAAGTTTGGAATGCCAGACAATTATGGATGGGAAGGCGACTAACGGGAATGGCTTAGATAAGCCCATTCCGTCTTGTTGTTTGAAGGCTAAGGCTTCAGATCCTGAGCTTGAGGCTCAATGCCATTCAACTGTTGTTTCTGGCTGGATTTCAGAATTTCAGTCTGGCTCTGATAAAGCTAGCAAGTTCGTTTATTTCAACAACCCAATGTGGCCGGGAGAAGCACATTCTCTGCAAGTAGAGAAGATACTATTTAAAGGAAAGTCAGAGTACCAAGAGGTCATGGTTTTTGAGTCTGCATCATATGGAAAAGTCCTTGTTCTTGATGGCATTGTTCAACTGACAGAGAAAGATGAATGTGCCTACCAGGAAATGATAGCGCATCTTCCTCTTTGTTCGATCCCGTCTCCTAAAACCGTTCTGGTTGTTGGCGGAGGTGATGGCGGAGTCCTGAGGGAAATTTCTCGTCACGACTCAGTGGAGCACATCGACATTTGTGAGATAGATGAGATGGTCATTAATGTGTCAAAACAATTTTTCCCCCAACTAGCTGTTGGATTCGAGGACCCTCGTGTTCACCTTCATGTTGGTGATGCTGTTGAATTTCTTCGGAATGCAAAGCAAGGGAAGTATGATGCCGTCATTGTTGATTCCTCTGATCCTGTGGGTCCTGCCCAAGAACTGGTGGAGATGCCATTTTTTCAGATGATAGCCCGAGCATTGCGACCTGGTGGTGTTCTCTGTAACATGGCTGAAAGTATGTGGCTGCATACACATCTCATTCAGGACATGATATCAATATGTCGCCAAACATTCAAGGATGTTCACTATGCATGGACCAGTGTTCCAACATATCCAAGTGGTGTTATAGGGTTTCTACTCTGCTCAACTGAAGGGCCAACAGTTGATTTTAGGAACCCTGTGAATCCCATCGAGAAGTTAGGACATCTCAAGTCAAAAAGAGAACTCAAATTCTACAACTCTGAGATGCACTTGGCTGCATTTGCGCTGCCATCGTTTCTCAAGAGCGAGGTGAAGGCACTTAGTGATGCTTCAGCTTCATTTGAAAAGGGAAGCATTTCATCCTAA

Protein sequence

MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFFQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASASFEKGSISS
Homology
BLAST of Cp4.1LG09g00380 vs. ExPASy Swiss-Prot
Match: Q94BN2 (Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1)

HSP 1 Score: 576.6 bits (1485), Expect = 2.0e-163
Identity = 277/356 (77.81%), Postives = 310/356 (87.08%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISE-F 60
           ME   G  L CQ  MDGKA+NGNGL+K +PSCCLKA A  PE +A+CHSTVVSGW SE  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
                K  K VYFNNPMWPGEAHSL+VEK+LFK KS++QEV+VFESA+YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFP+LAVGF+DPRV LH+GDA EFLR + +GKYDA+IVDSSDPVGPA  LVE PF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+ +ARAL+PGGVLCNMAESMWLHTHLI+DMISICRQTFK VHYAW+SVPTYPSGVIGF+
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 300

Query: 301 LCSTEGPTVDFRNPVNPIEKL-GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKAL 355
           LCSTEGP VDF+NP+NPIEKL G +  KRELKFYNS+MH AAFALP+FL+ EV +L
Sbjct: 301 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASL 356

BLAST of Cp4.1LG09g00380 vs. ExPASy Swiss-Prot
Match: Q9ZUB3 (Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 9.5e-126
Identity = 213/346 (61.56%), Postives = 271/346 (78.32%), Query Frame = 0

Query: 15  MDGKATNGNGLDKPIP------SCCLKAKASDPELEAQCHSTVVSGWISEFQSGSDKASK 74
           MD K T+   L +P        +  ++ +  D + E  C STV+ GW SE          
Sbjct: 1   MDAKETSATDLKRPREEDDNGGAATMETENGDQKKEPACFSTVIPGWFSEM--------- 60

Query: 75  FVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQLTEKDECAY 134
                +PMWPGEAHSL+VEK+LF+GKS+YQ+V+VF+SA+YGKVLVLDG++QLTE+DECAY
Sbjct: 61  -----SPMWPGEAHSLKVEKVLFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAY 120

Query: 135 QEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVINVSKQFFPQ 194
           QEMI HLPLCSIP+PK VLV+GGGDGGVLRE++RH S+E ID+CEID+MV++VSKQFFP 
Sbjct: 121 QEMITHLPLCSIPNPKKVLVIGGGDGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPD 180

Query: 195 LAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFFQMIARALR 254
           +A+G+EDPRV+L +GD V FL+NA +G YDAVIVDSSDP+GPA+EL E PFFQ +ARALR
Sbjct: 181 VAIGYEDPRVNLVIGDGVAFLKNAAEGSYDAVIVDSSDPIGPAKELFEKPFFQSVARALR 240

Query: 255 PGGVLCNMAESMWLHTHLIQDMISICRQTFK-DVHYAWTSVPTYPSGVIGFLLCSTEGPT 314
           PGGV+C  AES+WLH  +I+D++S CR+ FK  V+YAWTSVPTYPSGVIGF+LCSTEGP 
Sbjct: 241 PGGVVCTQAESLWLHMDIIEDIVSNCREIFKGSVNYAWTSVPTYPSGVIGFMLCSTEGPD 300

Query: 315 VDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKA 354
           VDF++P+NPI++    KS   LKFYN+E+H AAF LPSF K  +++
Sbjct: 301 VDFKHPLNPIDE-SSSKSNGPLKFYNAEIHSAAFCLPSFAKKVIES 331

BLAST of Cp4.1LG09g00380 vs. ExPASy Swiss-Prot
Match: O48659 (Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 445.7 bits (1145), Expect = 5.2e-124
Identity = 207/307 (67.43%), Postives = 254/307 (82.74%), Query Frame = 0

Query: 49  STVVSGWISEFQSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASY 108
           S+++ GW SE               +P+WPGEAHSL+VEKILF+GKS+YQ V+VF+S++Y
Sbjct: 13  SSILPGWFSEI--------------SPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTY 72

Query: 109 GKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEH 168
           GKVLVLDG++QLTE+DECAYQEMI HLPLCSIP+PK VLV+GGGDGGVLRE+SRH SVE 
Sbjct: 73  GKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQ 132

Query: 169 IDICEIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPV 228
           IDICEID+MVI+VSKQFFP +A+G+EDPRV LHVGD V FL+N  +G YDAVIVDSSDP+
Sbjct: 133 IDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPI 192

Query: 229 GPAQELVEMPFFQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFK-DVHYAWTS 288
           GPAQEL E PFF+ +ARAL PGGV+C  AES+WLH H+I+D++S CRQ FK  V+YAWT+
Sbjct: 193 GPAQELFEKPFFESVARALCPGGVVCTQAESIWLHMHIIEDIVSNCRQIFKGSVNYAWTT 252

Query: 289 VPTYPSGVIGFLLCSTEGPTVDFRNPVNPIE-KLGHLKSKRELKFYNSEMHLAAFALPSF 348
           VPTYPSGVIGF+LCSTEGP VDF+NP+NPI+    H K++  LKFYNSE+H A+F LPSF
Sbjct: 253 VPTYPSGVIGFMLCSTEGPAVDFKNPINPIDADDSHTKTRGPLKFYNSEIHSASFCLPSF 305

Query: 349 LKSEVKA 354
            K  +++
Sbjct: 313 AKRVIES 305

BLAST of Cp4.1LG09g00380 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 6.8e-124
Identity = 208/305 (68.20%), Postives = 253/305 (82.95%), Query Frame = 0

Query: 49  STVVSGWISEFQSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASY 108
           S+V+ GW SE               +PMWPGEAHSL+VEKILF+GKS+YQ VMVF+S++Y
Sbjct: 21  SSVIPGWFSEI--------------SPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTY 80

Query: 109 GKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEH 168
           GKVLVLDG++QLTE+DECAYQEMIAHLPLCSIPSPK VLV+GGGDGGVLRE++RH SVE 
Sbjct: 81  GKVLVLDGVIQLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARHLSVEQ 140

Query: 169 IDICEIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPV 228
           IDICEID+MV++VSKQFFP +AVGFEDPRV LH+GD V FL+   +G YDA+IVDSSDP+
Sbjct: 141 IDICEIDKMVVDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPI 200

Query: 229 GPAQELVEMPFFQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFK-DVHYAWTS 288
           GPAQEL E PFF+ +A+ALRPGGV+C  AES+WLH H+I+D+++ CRQ FK  V+YAWT+
Sbjct: 201 GPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTT 260

Query: 289 VPTYPSGVIGFLLCSTEGPTVDFRNPVNPIE-KLGHLKSKRELKFYNSEMHLAAFALPSF 348
           VPTYPSGVIGF+LCSTEGP VDF++P+NPI+   G  K+ + LKFYNSE+H AAF LPSF
Sbjct: 261 VPTYPSGVIGFMLCSTEGPPVDFKHPINPIDANDGRSKTMKPLKFYNSEIHSAAFCLPSF 311

Query: 349 LKSEV 352
            K  +
Sbjct: 321 AKKVI 311

BLAST of Cp4.1LG09g00380 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 8.9e-124
Identity = 205/307 (66.78%), Postives = 254/307 (82.74%), Query Frame = 0

Query: 49  STVVSGWISEFQSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASY 108
           S+++ GW SE               +P+WPGEAHSL+VEKILF+GKS+YQ VMVF+S++Y
Sbjct: 13  SSILPGWFSEI--------------SPLWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTY 72

Query: 109 GKVLVLDGIVQLTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEH 168
           GKVL+LDG++QLTE+DECAYQEMI HLPLCSIP+PK VLV+GGGDGGVLRE+SRH SVE 
Sbjct: 73  GKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQ 132

Query: 169 IDICEIDEMVINVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPV 228
           IDICEID+MVI+VSKQFFP +A+G+EDPRV LHVGD V FL+   +G YDAVIVDSSDP+
Sbjct: 133 IDICEIDKMVIDVSKQFFPNVAIGYEDPRVKLHVGDGVAFLKFVAEGTYDAVIVDSSDPI 192

Query: 229 GPAQELVEMPFFQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFK-DVHYAWTS 288
           GPAQEL E PFF+ +ARALRPGGV+C  AES+WLH H+I+D+++ CRQ FK  V+YAWT+
Sbjct: 193 GPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTT 252

Query: 289 VPTYPSGVIGFLLCSTEGPTVDFRNPVNPIE-KLGHLKSKRELKFYNSEMHLAAFALPSF 348
           VPTYPSGVIGF+LCSTEGP VDF+NP+NP++    H K++  LKFYNSE+H A+F LPSF
Sbjct: 253 VPTYPSGVIGFMLCSTEGPAVDFKNPINPVDADDSHTKTRGPLKFYNSEIHSASFCLPSF 305

Query: 349 LKSEVKA 354
            K  +++
Sbjct: 313 AKRVIES 305

BLAST of Cp4.1LG09g00380 vs. NCBI nr
Match: XP_023542101.1 (spermine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 749 bits (1934), Expect = 4.39e-273
Identity = 368/368 (100.00%), Postives = 368/368 (100.00%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ
Sbjct: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
           SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360
           CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS
Sbjct: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360

Query: 361 FEKGSISS 368
           FEKGSISS
Sbjct: 361 FEKGSISS 368

BLAST of Cp4.1LG09g00380 vs. NCBI nr
Match: XP_022944858.1 (spermine synthase-like [Cucurbita moschata])

HSP 1 Score: 736 bits (1899), Expect = 9.50e-268
Identity = 362/368 (98.37%), Postives = 363/368 (98.64%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDVTGRSLECQTIMDGKATNGNGLDK IPSCCLKAKASDPELEAQCHSTVVSGWISEFQ
Sbjct: 1   MEDVTGRSLECQTIMDGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
           SGSDKASKFVYFNNPMWPGEAHSLQVE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  SGSDKASKFVYFNNPMWPGEAHSLQVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIP PKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPFPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           Q IARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 QTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360
           CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS
Sbjct: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360

Query: 361 FEKGSISS 368
            +KGSISS
Sbjct: 361 LKKGSISS 368

BLAST of Cp4.1LG09g00380 vs. NCBI nr
Match: KAG7013301.1 (Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 733 bits (1893), Expect = 8.11e-267
Identity = 363/369 (98.37%), Postives = 364/369 (98.64%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMD-GKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEF 60
           MEDVTGRSLECQTIMD GKATNGNGLDK IPSCCLKAKASDPELEAQCHSTVVSGWISEF
Sbjct: 1   MEDVTGRSLECQTIMDDGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEF 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
           QSGSDKASKFVYFNNPMWPGEAHSLQVE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQ
Sbjct: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF
Sbjct: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           FQ IARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL
Sbjct: 241 FQTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300

Query: 301 LCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASA 360
           LCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASA
Sbjct: 301 LCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASA 360

Query: 361 SFEKGSISS 368
           S +KGSISS
Sbjct: 361 SLKKGSISS 369

BLAST of Cp4.1LG09g00380 vs. NCBI nr
Match: XP_022968621.1 (spermine synthase-like [Cucurbita maxima])

HSP 1 Score: 730 bits (1884), Expect = 1.84e-265
Identity = 358/368 (97.28%), Postives = 360/368 (97.83%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDVTGRS ECQTIMDGK TNGNGLDK IPSCCLKAKASDPELEAQCHSTVVSGWISEFQ
Sbjct: 1   MEDVTGRSFECQTIMDGKTTNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
            GSDKASKFVYFNNPMWPGEAHSL VE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  PGSDKASKFVYFNNPMWPGEAHSLLVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDA+IVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAIIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           Q IARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 QTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360
           CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS
Sbjct: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360

Query: 361 FEKGSISS 368
            +KGSISS
Sbjct: 361 LKKGSISS 368

BLAST of Cp4.1LG09g00380 vs. NCBI nr
Match: KAG6574234.1 (Spermine synthase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 707 bits (1826), Expect = 7.81e-257
Identity = 348/353 (98.58%), Postives = 349/353 (98.87%), Query Frame = 0

Query: 16  DGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQSGSDKASKFVYFNNP 75
           DGKATNGNGLDK IPSCCLKAKASDPELEAQCHSTVVSGWISEFQSGSDKASKFVYFNNP
Sbjct: 3   DGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQSGSDKASKFVYFNNP 62

Query: 76  MWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHL 135
           MWPGEAHSLQVE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHL
Sbjct: 63  MWPGEAHSLQVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQLTEKDECAYQEMIAHL 122

Query: 136 PLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVINVSKQFFPQLAVGFED 195
           PLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVINVSKQFFPQLAVGFED
Sbjct: 123 PLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVINVSKQFFPQLAVGFED 182

Query: 196 PRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFFQMIARALRPGGVLCN 255
           PRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFFQ IARALRPGGVLCN
Sbjct: 183 PRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFFQTIARALRPGGVLCN 242

Query: 256 MAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFRNPVN 315
           MAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFRNPVN
Sbjct: 243 MAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLLCSTEGPTVDFRNPVN 302

Query: 316 PIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASASFEKGSISS 368
           PIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS +KGSISS
Sbjct: 303 PIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASASLKKGSISS 355

BLAST of Cp4.1LG09g00380 vs. ExPASy TrEMBL
Match: A0A6J1FZ77 (spermine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111449258 PE=3 SV=1)

HSP 1 Score: 736 bits (1899), Expect = 4.60e-268
Identity = 362/368 (98.37%), Postives = 363/368 (98.64%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDVTGRSLECQTIMDGKATNGNGLDK IPSCCLKAKASDPELEAQCHSTVVSGWISEFQ
Sbjct: 1   MEDVTGRSLECQTIMDGKATNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
           SGSDKASKFVYFNNPMWPGEAHSLQVE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  SGSDKASKFVYFNNPMWPGEAHSLQVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIP PKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPFPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           Q IARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 QTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360
           CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS
Sbjct: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360

Query: 361 FEKGSISS 368
            +KGSISS
Sbjct: 361 LKKGSISS 368

BLAST of Cp4.1LG09g00380 vs. ExPASy TrEMBL
Match: A0A6J1HVE7 (spermine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111467782 PE=3 SV=1)

HSP 1 Score: 730 bits (1884), Expect = 8.90e-266
Identity = 358/368 (97.28%), Postives = 360/368 (97.83%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDVTGRS ECQTIMDGK TNGNGLDK IPSCCLKAKASDPELEAQCHSTVVSGWISEFQ
Sbjct: 1   MEDVTGRSFECQTIMDGKTTNGNGLDKAIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
            GSDKASKFVYFNNPMWPGEAHSL VE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  PGSDKASKFVYFNNPMWPGEAHSLLVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDA+IVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAIIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           Q IARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 QTIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360
           CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS
Sbjct: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360

Query: 361 FEKGSISS 368
            +KGSISS
Sbjct: 361 LKKGSISS 368

BLAST of Cp4.1LG09g00380 vs. ExPASy TrEMBL
Match: A0A6J1CJE6 (spermine synthase-like OS=Momordica charantia OX=3673 GN=LOC111011708 PE=3 SV=1)

HSP 1 Score: 689 bits (1778), Expect = 1.38e-249
Identity = 341/370 (92.16%), Postives = 349/370 (94.32%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDV GRSLECQ IMDGKATNGNGLD  IPSCCLKAK SDPELEAQCHSTVVSGW SEFQ
Sbjct: 1   MEDVAGRSLECQKIMDGKATNGNGLDGAIPSCCLKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
           SGSDKASK VYFNNPMWPGEAH+LQVE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  SGSDKASKNVYFNNPMWPGEAHALQVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEID+MVI+
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDKMVID 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSK+FFPQLAVGFEDPRVHLHVGDAVEFLRNA+QGKYDA+IVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNARQGKYDAIIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           Q IARALR GGVLCNMAESMWLHTHLIQDMISICR+TFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 QTIARALRSGGVLCNMAESMWLHTHLIQDMISICRETFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKL--GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDAS 360
           CSTEGPTVDFRNPVNPIE L   HLKSKRELKFYNSEMH AAFALPSFLK EVKALSDA 
Sbjct: 301 CSTEGPTVDFRNPVNPIENLVGQHLKSKRELKFYNSEMHSAAFALPSFLKREVKALSDAP 360

Query: 361 ASFEKGSISS 368
            S + G ISS
Sbjct: 361 VSGKNGWISS 370

BLAST of Cp4.1LG09g00380 vs. ExPASy TrEMBL
Match: A0A0A0LC47 (PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G604620 PE=3 SV=1)

HSP 1 Score: 681 bits (1756), Expect = 2.87e-246
Identity = 331/368 (89.95%), Postives = 347/368 (94.29%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEFQ 60
           MEDVTG+SLECQ IMDGK TNGNG +K IPSCCLKAK SDPELEAQCHSTVVSGW SEFQ
Sbjct: 1   MEDVTGKSLECQKIMDGKVTNGNGREKVIPSCCLKAKTSDPELEAQCHSTVVSGWFSEFQ 60

Query: 61  SGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120
           SGSDKA K +YFNNPMWPGEAH+L+VE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQL
Sbjct: 61  SGSDKACKKLYFNNPMWPGEAHALEVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQL 120

Query: 121 TEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVIN 180
           TEKDECAYQEMIAHLPLCSIPSPK+VLVVGGGDGGVLREISRHDSVEHIDICEID+MVI+
Sbjct: 121 TEKDECAYQEMIAHLPLCSIPSPKSVLVVGGGDGGVLREISRHDSVEHIDICEIDQMVID 180

Query: 181 VSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPFF 240
           VSK+ FPQLAVGFEDPRVHLHVGDAVEFLRN KQGKYDA+IVDSSDPVGPAQELVEMPFF
Sbjct: 181 VSKKCFPQLAVGFEDPRVHLHVGDAVEFLRNGKQGKYDAIIVDSSDPVGPAQELVEMPFF 240

Query: 241 QMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300
           +MIA+ALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL
Sbjct: 241 RMIAQALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFLL 300

Query: 301 CSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASAS 360
           CSTEGP VDF NPVNPIE +GH KSKRELKFYNSEMH AAFALPSFLK EV+AL+DA AS
Sbjct: 301 CSTEGPKVDFSNPVNPIENVGHPKSKRELKFYNSEMHSAAFALPSFLKREVQALNDAPAS 360

Query: 361 FEKGSISS 368
            + G ISS
Sbjct: 361 GKNGCISS 368

BLAST of Cp4.1LG09g00380 vs. ExPASy TrEMBL
Match: A0A5D3BTV3 (Spermine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00270 PE=3 SV=1)

HSP 1 Score: 678 bits (1749), Expect = 3.35e-245
Identity = 334/369 (90.51%), Postives = 349/369 (94.58%), Query Frame = 0

Query: 1   MEDVTGRSL-ECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISEF 60
           MEDVTG+SL ECQ +MDGK TNGNG +  IPSCCLKAK SDP+LEAQCHSTVVSGW SEF
Sbjct: 1   MEDVTGKSLVECQKVMDGKVTNGNGKEV-IPSCCLKAKTSDPKLEAQCHSTVVSGWFSEF 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
           QSGSDKA K +YFNNPMWPGEAH+L+VE ILFKGKSEYQEVMVFESASYGKVLVLDGIVQ
Sbjct: 61  QSGSDKACKKLYFNNPMWPGEAHALEVENILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSIPSPK+VLVVGGGDGGVLREISRHDSVEHIDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSIPSPKSVLVVGGGDGGVLREISRHDSVEHIDICEIDQMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDA+IVDSSDPVGPAQELVEMPF
Sbjct: 181 DVSKKFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAIIVDSSDPVGPAQELVEMPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+M A+ALRPGGVLCNMAESMWLHTHLIQDMISIC QTFKDVHYAWTSVPTYPSGVIGFL
Sbjct: 241 FRMTAQALRPGGVLCNMAESMWLHTHLIQDMISICCQTFKDVHYAWTSVPTYPSGVIGFL 300

Query: 301 LCSTEGPTVDFRNPVNPIEKLGHLKSKRELKFYNSEMHLAAFALPSFLKSEVKALSDASA 360
           LCSTEGP VDFRNPVNPIEKLGHLKSKRELKFYNSEMH AAFALPSFLK EVKALSDA A
Sbjct: 301 LCSTEGPKVDFRNPVNPIEKLGHLKSKRELKFYNSEMHSAAFALPSFLKREVKALSDAPA 360

Query: 361 SFEKGSISS 368
           S + G ISS
Sbjct: 361 SGKNGCISS 368

BLAST of Cp4.1LG09g00380 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-164
Identity = 277/356 (77.81%), Postives = 310/356 (87.08%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISE-F 60
           ME   G  L CQ  MDGKA+NGNGL+K +PSCCLKA A  PE +A+CHSTVVSGW SE  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
                K  K VYFNNPMWPGEAHSL+VEK+LFK KS++QEV+VFESA+YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFP+LAVGF+DPRV LH+GDA EFLR + +GKYDA+IVDSSDPVGPA  LVE PF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+ +ARAL+PGGVLCNMAESMWLHTHLI+DMISICRQTFK VHYAW+SVPTYPSGVIGF+
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 300

Query: 301 LCSTEGPTVDFRNPVNPIEKL-GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKAL 355
           LCSTEGP VDF+NP+NPIEKL G +  KRELKFYNS+MH AAFALP+FL+ EV +L
Sbjct: 301 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASL 356

BLAST of Cp4.1LG09g00380 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-164
Identity = 277/356 (77.81%), Postives = 310/356 (87.08%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISE-F 60
           ME   G  L CQ  MDGKA+NGNGL+K +PSCCLKA A  PE +A+CHSTVVSGW SE  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
                K  K VYFNNPMWPGEAHSL+VEK+LFK KS++QEV+VFESA+YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFP+LAVGF+DPRV LH+GDA EFLR + +GKYDA+IVDSSDPVGPA  LVE PF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+ +ARAL+PGGVLCNMAESMWLHTHLI+DMISICRQTFK VHYAW+SVPTYPSGVIGF+
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 300

Query: 301 LCSTEGPTVDFRNPVNPIEKL-GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKAL 355
           LCSTEGP VDF+NP+NPIEKL G +  KRELKFYNS+MH AAFALP+FL+ EV +L
Sbjct: 301 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASL 356

BLAST of Cp4.1LG09g00380 vs. TAIR 10
Match: AT5G53120.3 (spermidine synthase 3 )

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-164
Identity = 277/356 (77.81%), Postives = 310/356 (87.08%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISE-F 60
           ME   G  L CQ  MDGKA+NGNGL+K +PSCCLKA A  PE +A+CHSTVVSGW SE  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
                K  K VYFNNPMWPGEAHSL+VEK+LFK KS++QEV+VFESA+YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFP+LAVGF+DPRV LH+GDA EFLR + +GKYDA+IVDSSDPVGPA  LVE PF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+ +ARAL+PGGVLCNMAESMWLHTHLI+DMISICRQTFK VHYAW+SVPTYPSGVIGF+
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 300

Query: 301 LCSTEGPTVDFRNPVNPIEKL-GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKAL 355
           LCSTEGP VDF+NP+NPIEKL G +  KRELKFYNS+MH AAFALP+FL+ EV +L
Sbjct: 301 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASL 356

BLAST of Cp4.1LG09g00380 vs. TAIR 10
Match: AT5G53120.4 (spermidine synthase 3 )

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-164
Identity = 277/356 (77.81%), Postives = 310/356 (87.08%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISE-F 60
           ME   G  L CQ  MDGKA+NGNGL+K +PSCCLKA A  PE +A+CHSTVVSGW SE  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
                K  K VYFNNPMWPGEAHSL+VEK+LFK KS++QEV+VFESA+YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFP+LAVGF+DPRV LH+GDA EFLR + +GKYDA+IVDSSDPVGPA  LVE PF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+ +ARAL+PGGVLCNMAESMWLHTHLI+DMISICRQTFK VHYAW+SVPTYPSGVIGF+
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 300

Query: 301 LCSTEGPTVDFRNPVNPIEKL-GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKAL 355
           LCSTEGP VDF+NP+NPIEKL G +  KRELKFYNS+MH AAFALP+FL+ EV +L
Sbjct: 301 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASL 356

BLAST of Cp4.1LG09g00380 vs. TAIR 10
Match: AT5G53120.5 (spermidine synthase 3 )

HSP 1 Score: 576.6 bits (1485), Expect = 1.4e-164
Identity = 277/356 (77.81%), Postives = 310/356 (87.08%), Query Frame = 0

Query: 1   MEDVTGRSLECQTIMDGKATNGNGLDKPIPSCCLKAKASDPELEAQCHSTVVSGWISE-F 60
           ME   G  L CQ  MDGKA+NGNGL+K +PSCCLKA A  PE +A+CHSTVVSGW SE  
Sbjct: 1   MEGDVGIGLVCQNTMDGKASNGNGLEKTVPSCCLKAMACVPEDDAKCHSTVVSGWFSEPH 60

Query: 61  QSGSDKASKFVYFNNPMWPGEAHSLQVEKILFKGKSEYQEVMVFESASYGKVLVLDGIVQ 120
                K  K VYFNNPMWPGEAHSL+VEK+LFK KS++QEV+VFESA+YGKVLVLDGIVQ
Sbjct: 61  PRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQEVLVFESATYGKVLVLDGIVQ 120

Query: 121 LTEKDECAYQEMIAHLPLCSIPSPKTVLVVGGGDGGVLREISRHDSVEHIDICEIDEMVI 180
           LTEKDECAYQEMIAHLPLCSI SPK VLVVGGGDGGVLREISRH SVE IDICEID+MVI
Sbjct: 121 LTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLREISRHSSVEVIDICEIDKMVI 180

Query: 181 NVSKQFFPQLAVGFEDPRVHLHVGDAVEFLRNAKQGKYDAVIVDSSDPVGPAQELVEMPF 240
           +VSK+FFP+LAVGF+DPRV LH+GDA EFLR + +GKYDA+IVDSSDPVGPA  LVE PF
Sbjct: 181 DVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDAIIVDSSDPVGPALALVEKPF 240

Query: 241 FQMIARALRPGGVLCNMAESMWLHTHLIQDMISICRQTFKDVHYAWTSVPTYPSGVIGFL 300
           F+ +ARAL+PGGVLCNMAESMWLHTHLI+DMISICRQTFK VHYAW+SVPTYPSGVIGF+
Sbjct: 241 FETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFKSVHYAWSSVPTYPSGVIGFV 300

Query: 301 LCSTEGPTVDFRNPVNPIEKL-GHLKSKRELKFYNSEMHLAAFALPSFLKSEVKAL 355
           LCSTEGP VDF+NP+NPIEKL G +  KRELKFYNS+MH AAFALP+FL+ EV +L
Sbjct: 301 LCSTEGPAVDFKNPINPIEKLDGAMTHKRELKFYNSDMHRAAFALPTFLRREVASL 356

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94BN22.0e-16377.81Spermine synthase OS=Arabidopsis thaliana OX=3702 GN=SPMS PE=1 SV=1[more]
Q9ZUB39.5e-12661.56Spermidine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=SPDSYN1 PE=1 SV=1[more]
O486595.2e-12467.43Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1[more]
O821476.8e-12468.20Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1[more]
Q965568.9e-12466.78Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_023542101.14.39e-273100.00spermine synthase-like [Cucurbita pepo subsp. pepo][more]
XP_022944858.19.50e-26898.37spermine synthase-like [Cucurbita moschata][more]
KAG7013301.18.11e-26798.37Spermine synthase [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022968621.11.84e-26597.28spermine synthase-like [Cucurbita maxima][more]
KAG6574234.17.81e-25798.58Spermine synthase, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1FZ774.60e-26898.37spermine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111449258 PE=3 SV=1[more]
A0A6J1HVE78.90e-26697.28spermine synthase-like OS=Cucurbita maxima OX=3661 GN=LOC111467782 PE=3 SV=1[more]
A0A6J1CJE61.38e-24992.16spermine synthase-like OS=Momordica charantia OX=3673 GN=LOC111011708 PE=3 SV=1[more]
A0A0A0LC472.87e-24689.95PABS domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G604620 PE=3 S... [more]
A0A5D3BTV33.35e-24590.51Spermine synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
Match NameE-valueIdentityDescription
AT5G53120.11.4e-16477.81spermidine synthase 3 [more]
AT5G53120.21.4e-16477.81spermidine synthase 3 [more]
AT5G53120.31.4e-16477.81spermidine synthase 3 [more]
AT5G53120.41.4e-16477.81spermidine synthase 3 [more]
AT5G53120.51.4e-16477.81spermidine synthase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 120..355
e-value: 2.5E-94
score: 317.1
NoneNo IPR availablePFAMPF01564Spermine_synthcoord: 125..311
e-value: 3.3E-65
score: 219.0
NoneNo IPR availablePANTHERPTHR11558:SF25SPERMINE SYNTHASEcoord: 1..360
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 167..253
e-value: 6.39812E-10
score: 53.9731
IPR037163Spermidine synthase, tetramerisation domain superfamilyGENE3D2.30.140.10Spermidine synthase, tetramerisation domaincoord: 46..119
e-value: 9.6E-28
score: 98.4
IPR035246Spermidine synthase, tetramerisation domainPFAMPF17284Spermine_synt_Ncoord: 75..122
e-value: 1.6E-20
score: 72.7
IPR030668Spermidine/spermine synthase, eukaryotesPIRSFPIRSF000502Spermidine_synthcoord: 42..356
e-value: 1.9E-138
score: 458.2
IPR001045Spermidine/spermine synthasesTIGRFAMTIGR00417TIGR00417coord: 79..346
e-value: 4.6E-93
score: 309.3
IPR001045Spermidine/spermine synthasesPANTHERPTHR11558SPERMIDINE/SPERMINE SYNTHASEcoord: 1..360
IPR001045Spermidine/spermine synthasesHAMAPMF_00198Spermidine_synthcoord: 54..352
score: 34.570503
IPR030373Polyamine biosynthesis domain, conserved sitePROSITEPS01330PABS_1coord: 146..159
IPR030374Polyamine biosynthesis domainPROSITEPS51006PABS_2coord: 53..303
score: 95.963814
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 71..350

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g00380.1Cp4.1LG09g00380.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006596 polyamine biosynthetic process
biological_process GO:0006595 polyamine metabolic process
molecular_function GO:0004766 spermidine synthase activity
molecular_function GO:0003824 catalytic activity