Cp4.1LG09g00260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g00260
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description45 kDa calcium-binding protein isoform X2
LocationCp4.1LG09: 135897 .. 141129 (-)
RNA-Seq ExpressionCp4.1LG09g00260
SyntenyCp4.1LG09g00260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATGTTGTTATTATATCAGACGCACAGAGTTGAAGCAGCCTGGCAGAAGCACTTTTGTCTGTCACCATTCATGTTCTTTTCGTAATAATTTCCCTGCAAACTCCTCCCTCCTTCTACTTCCAACTTTCCACCTCTTTTTGAAGTGCTTTCTATTTATATGAACCCAACCCTCAGCTTCATGCATACCATTCACTATCACTCAATCATGCTCAACACTAAATGTGAATTTCTGTATCCTCTTTCCAACTTTTCTTGCTCAAATGAGTATTCCAAACAGAGCAGCAGATGCAAATGGAAACTAATCAACCCTTTTTCTTTCTTTTTATGATGCTCCTGCTGCTTCTTTCTGCGATGCTCATTTCAGCAAGAAATGTTGCAAAATTGGAGGCTTTTTGCACCATTCCTCGCTTTCCTCTCATCTGGGTTATCTCTTCTTTGCATCTTCTTTCTCTCATCTGGGTTATCTCTTCTTTGCATCTTCTTTCTCTCATCTGGGTTATCTCTTCTTTGCACGTTTAAGCATTAAACTAAGCGGTATTGTAAAACAGTAGCCGCCATTTGTGCTGCAAAAATGTGTTTATAAATGGGATTAACGAGGAAGGAAAGAAGCTGGAATTTCTATTGTTAATGTAAATGGTAATGAATGGTCAATGATTGTTGATGATTTAATGTGGTTTATAAATGGAAAGAGACCCATTCTTTTGTTTTGTCTGTGGATAACTTGTCGCCTATTTTAGAATACAAAAGTTGTCAGCCTCAGAATGGATTCCGCCATTGTCTAATATTGCAAGCTTAGTGAACGGTGCTCCGTTCACCGTCTTCCCCCATCCTAATCTCCGATATTTTCACTACTATTTCGTCAATGGCTTCTGTAGATAATCGTTTTCGCCATCAAGGTTATTGCATTGGAATCCCACCTGATCCAGGTTTCTATCTTGTCCTGAACTTGTCATTTTCGGCGTCCTCAAATGGGGAGGGCGGTGGTTTATGTTCTTATATTCGCCGCCTTCGCTGCTTTCTTCGTATTTTCTCCGTCCGATCTCCCCGATCGGAACCGTCAAGAACTAACTCGTCGCCTAGGTTACAAATTCCGAACCCCCACTTTTGATCCTCTTGTGGCAGAATTGGAAAGAATCGCAGAGGAACGTGAATTGGGAAGCCGAGGAAACGTGATTGGAGTGGAACAAACTGCGAAGGCAAAGATTCCCGAGGCTTACAGAGATTCCAACGACGAAGGGAGATTGAACATTTCGTCGAGGTTGGTCGTATTGTTTCCTTTGGTGGACCACTCCCCAAAAGATGGGCTTATCAACTACGAGGAGATGAGCAATTGGATTACTGAGCAAACCATCAACAAATTGAACAGCAGAACGGGGAGAGAGTTAGCGTTCTACGACAAGAACGGAGATGGCGCCATATCTTTCCACGAATATCTGCCGCAGTTTACAGAGGAAGATATTGGTAAGCCTTTTGGTTTCTTTAATGCTGGGGAAGTTCAAGAAATTCCCATTTTCCTGGAAACTAGAATGATGATAGATCAAAACAAACCATGGTTTTCTGGGATTGAAATGAACATTGTGAAACAGCTAGAAACGAAACGGGGCATGGACAAGCTGGATGGTGGAAACAGCAATTCACAAATGCTGATGTTGACCCAAATGGATTGCTCAGTTTTGATGAACTGAAAGAGTAATGTACCATTTTTAACCATTAATTTGAAACATGTGTTTCCTTGTAAGAATGGAATGTGATCTTTGTGTGCTGCAGTTTCCTGCACCCACAAGACAGCAGCAATTACCGAATACAAAACTGGCTGTTGAGCGAGAAAATGAAGTAACCTTTCCCCATACTCAAGCTCTTAACTTTTGCTATCTGCATTGCAAATCAAAGCATCAGCTTGTGTTTAATATGTGCAGGCGCATGGACTATGACAGAGATGGAAAACTCAGTTTTGATGAATTCCTCCACCACGCATACGACATATACAAGAGCTATATTGAGTTCGAAACTCAAGGGGACGACGTACCGACCGCGGAAGAGAAATTTGATGAGCTTGATCTTGATGAGGATGAGTATGCATTTGACCTATATTTCTGCTTCATACCAACGTGTTGGGTGTTTATAATATCAAAAAATTGTGTGCAGAGTATTGACAGTGGAAGAATTAAGGCCATTATTCCAGTATCTTAATCCTGGAGAACTTTCTCATGCCCAACATTACGCAGCTCATTTGATTAATGAGGTATATTAGTAGTTGATTTGAAGAGATTAAGAAAACAAACATGATGATTCACTCGATTTTATGCTGTAATTAACGCTGCAGGCTGATGATAACAAGGATGGGTTCTTAACACTCGAGGAGATGCTTAAACACGAGTACGTATTCTACACCACACTATATGAAAATCAAAATGGCGACTATGAAGACGATTTCCATGATGAACTTTGATGGTTTTTTTCTTCTTTTTTTTTTTTTTTCTTTTCTTTTCTTTTCTTAAGCTTAGATTTTAGATTGGATTTGATGATGGGTTGCATAGGGGTGAAGGAAATAAACAATATAAAATGGTCTGGGTTACTATTAACTCACCTACCCTCTAAATTTTGATTCATTTTCCATTGTTTTCCTTCAAATGTAAAGAATAGCCTCTCGTCTATGCTGTATATTAAAATGAATTATGGTAGACAGATTTTCTGTCCCCATTTTTTTCTTTCTTTTTTCCAATTCCATTTATTGTAAATCATTTTATTTTCTTTTTTCTTTTTTCATTTCGCCCATGGTGCTCATCATATTGTTTCATGTTTATTTATTTATATACCTGTTATCTAGGGGGGGAAAAAACTCAATTTTAGCATGCAATTATATGCTTTATTTCAGTTGTGTATGGGTTTTAAGATTTTATGCTAACTAGTTTTATAGAGTTATAACATTCAACTTGAAACGGATGACATCAACCCCTATAGATAGATAGATAGATCTACAATGATTAAAGAGACAAATATGAACTCAAATGCCTTTGATTGATGTATTGGAACTTTAGTAACTACCTGCGTTGTTGATTCTTGAATATTATAGGTTCTGTTTTGAGTTTTCTCAAGATATATATTTTTTTATAAAAAAAAAAAGTAATCTTCTGTCGTTCCACTTCCAAATGGTCCTTGCCACAATGTTATACATGGGAGGCAGCTTAGCCTAACAGAAAATGGCTGCTGCTATTGGAGGTCTTTTTTTATGGAGGCCACCTTCCATCATGTTTGAGTACTTTTCAATGAAAACATTTAAAAAGAAAAAAGGCTTTAAGTTGGCTCTGATGAAGGGCAGACAAGGCAGGCAGTGCATGCAACTTTAGAAACCATTCCTTTCTTTCTTACACGAAGAAGTAGAAAGATCAGTCATGGCCTCCAAAATCACTGCCTTCCTTCTCTACAGGTAACATAATCTTTCAACATCACAGAAATCGCTCAGTGCCATAGCTTAAGAGACAGATAACCTCCCTTTTTTCTTCCTTCCATTGCAGGAGAGTTAGGGGCCTCCAATACTAAGATGAATCTTAATCACTCTCCTAACAAGGTATACTGTAAAGTGGTGAATATGTGGTTTTTCATGTTTGGAAGCTATTTACTTTTCTCTTTAACAACTTCTCTAGTCCCTACTGTCAGAGACATGAGAGATCCAGATATTATACAAGTATATAGTTTTATGTCAACAGATTCGTTAAGAACAGTTCTGATGAGTTTGATCTGTTTCAGGAATCTGACCTGGATGAGAATTTCTGAGCTGTTGTGAGTAATTTTCACTCAGGAAAGTTATACAATGTGTATTGTGTTGGTAACCTTGCAGTAATCTTTTCTTCCATGAAGGATGGATTCCAAACAGAGATGAGGAAGATTTTGATGGTTGACAGACCAGATTATGATGATCCTGTAGGTAACCGTAAGCATGAGCCTCAGAAACCAGGCACTGGAAAGACATAGGACTTGAGATTCCTGTGTTTCCCTTGGGCAAATAATGAGGATGAAAATGGGAACTCTCCTGATAGTCTCCTTGTGAACCAAATAAGACTTCCAAATTGAAAAGTTCTGAATCCTAATGGAAGGTGTAGAATGAATCAACAAGTTGTAGTGCAGTTTTAGAGGCATGATAGTTAAGGCGTCAAACTCTTGTGAGGGTAGGTTAAGTCATCTAAACTAGCTAAGATGTGATACATTGTATAACTATTCACTTACACTTTACAGATGAAAAGTATGGAAGAATAAGCTGATTATACAACAGTGGGACACACAAACTTTAAAGACAGAAAATAGAGGACGCCCACCAAATACCGAAAGAAACGACCCCAATCGTACCAAAAGCTTGAGCCACTTCAAATATGACGTTGTCTTGCTGGAGGGGCAAATTCTGCCAATCTGTCCGCCTGCATCTCATCTCCAGTTAGTTTAAATCATATTTGTATTGGAAGAACTCCCACAACAACAAATAAGACAGTCTAATAACTGAGATCGAGTTTCGTTAGCTGCTGTATTTAGCTTCTCAGAAGAAAGTTTATTGTTTCTATGCATTTGGAAACAAGCAAAGCCATTTGAACTTTTATTCTTTTAATGCCTAGTATTGTAACTGTCTCCTCATTTGCCATGTTTATACTTAAGTGTACTCACCCCAAATCAGCCATGATAATGCTGATATTGTCTTGTGACCCTTTGTCCTACAAGATACAAACATAATATTTGCTTTATTAGAACTGTAAACCAAGTTCTTGTTATTAGAAGCTTTAAACAAATCAAATTGTGCGAGTATTACCAAGGCTGCTTGTGCGAGGGCTTCACAAGCTAACTGAAAATCAATGTAAATGAGAAGTTAGAAGTTGAACCATAACCAAACAGAGACAGCAAGAAATAGAGTGCGTAAAAGAATAAAGAATACAAGTAACATGATGTACACCTGAACATCACCATGCTGACTCAATTGATTCCGAACAAACTTAACCGCATCGGAGCTGATACAACACCCGAAAATGGCACTTATTTAGTCTGTCTAGATCAAAGTTGATGGACAATCTGATAAATCTTTAGGACATAAAGACTGAAGCCTAGAAATGACTGAGATACGAAAAATGACCTATTCATGTAATCCCATAGGCCATCCGATGCTATCAGTACAAACTCGACATTTGATCCGAGGGTAAGCTGAAGAACTTCTGGTGATGCGGTTACCAAATCTCCATTGAACTGTACACTG

mRNA sequence

TATGTTGTTATTATATCAGACGCACAGAGTTGAAGCAGCCTGGCAGAAGCACTTTTGTCTGTCACCATTCATGTTCTTTTCGTAATAATTTCCCTGCAAACTCCTCCCTCCTTCTACTTCCAACTTTCCACCTCTTTTTGAAGTGCTTTCTATTTATATGAACCCAACCCTCAGCTTCATGCATACCATTCACTATCACTCAATCATGCTCAACACTAAATGTGAATTTCTGTATCCTCTTTCCAACTTTTCTTGCTCAAATGAGTATTCCAAACAGAGCAGCAGATGCAAATGGAAACTAATCAACCCTTTTTCTTTCTTTTTATGATGCTCCTGCTGCTTCTTTCTGCGATGCTCATTTCAGCAAGAAATGTTGCAAAATTGGAGGCTTTTTGCACCATTCCTCGCTTTCCTCTCATCTGGGTTATCTCTTCTTTGCATCTTCTTTCTCTCATCTGGGTTATCTCTTCTTTGCATCTTCTTTCTCTCATCTGGGTTATCTCTTCTTTGCACGTTTAAGCATTAAACTAAGCGGTATTGTAAAACAGTAGCCGCCATTTGTGCTGCAAAAATGTGTTTATAAATGGGATTAACGAGGAAGGAAAGAAGCTGGAATTTCTATTGTTAATGTAAATGGTAATGAATGGTCAATGATTGTTGATGATTTAATGTGGTTTATAAATGGAAAGAGACCCATTCTTTTGTTTTGTCTGTGGATAACTTGTCGCCTATTTTAGAATACAAAAGTTGTCAGCCTCAGAATGGATTCCGCCATTGTCTAATATTGCAAGCTTAGTGAACGGTGCTCCGTTCACCGTCTTCCCCCATCCTAATCTCCGATATTTTCACTACTATTTCGTCAATGGCTTCTGTAGATAATCGTTTTCGCCATCAAGGTTATTGCATTGGAATCCCACCTGATCCAGGTTTCTATCTTGTCCTGAACTTGTCATTTTCGGCGTCCTCAAATGGGGAGGGCGGTGGTTTATGTTCTTATATTCGCCGCCTTCGCTGCTTTCTTCGTATTTTCTCCGTCCGATCTCCCCGATCGGAACCGTCAAGAACTAACTCGTCGCCTAGGTTACAAATTCCGAACCCCCACTTTTGATCCTCTTGTGGCAGAATTGGAAAGAATCGCAGAGGAACGTGAATTGGGAAGCCGAGGAAACGTGATTGGAGTGGAACAAACTGCGAAGGCAAAGATTCCCGAGGCTTACAGAGATTCCAACGACGAAGGGAGATTGAACATTTCGTCGAGGTTGGTCGTATTGTTTCCTTTGGTGGACCACTCCCCAAAAGATGGGCTTATCAACTACGAGGAGATGAGCAATTGGATTACTGAGCAAACCATCAACAAATTGAACAGCAGAACGGGGAGAGAGTTAGCGTTCTACGACAAGAACGGAGATGGCGCCATATCTTTCCACGAATATCTGCCGCAGTTTACAGAGGAAGATATTGCTAGAAACGAAACGGGGCATGGACAAGCTGGATGGTGGAAACAGCAATTCACAAATGCTGATGTTGACCCAAATGGATTGCTCAGTTTTGATGAACTGAAAGATTTCCTGCACCCACAAGACAGCAGCAATTACCGAATACAAAACTGGCTGTTGAGCGAGAAAATGAAGCGCATGGACTATGACAGAGATGGAAAACTCAGTTTTGATGAATTCCTCCACCACGCATACGACATATACAAGAGCTATATTGAGTTCGAAACTCAAGGGGACGACGTACCGACCGCGGAAGAGAAATTTGATGAGCTTGATCTTGATGAGGATGAAGTATTGACAGTGGAAGAATTAAGGCCATTATTCCAGTATCTTAATCCTGGAGAACTTTCTCATGCCCAACATTACGCAGCTCATTTGATTAATGAGGCTGATGATAACAAGGATGGGTTCTTAACACTCGAGGAGATGCTTAAACACGAGTACGTATTCTACACCACACTATATGAAAATCAAAATGGCGACTATGAAGACGATTTCCATGATGAACTTTGATGGTTTTTTTCTTCTTTTTTTTTTTTTTTCTTTTCTTTTCTTTTCTTAAGCTTAGATTTTAGATTGGATTTGATGATGGGTTGCATAGGGGTGAAGGAAATAAACAATATAAAATGGTCTGGGTTACTATTAACTCACCTACCCTCTAAATTTTGATTCATTTTCCATTGTTTTCCTTCAAATGTAAAGAATAGCCTCTCGTCTATGCTGTATATTAAAATGAATTATGGTAGACAGATTTTCTGTCCCCATTTTTTTCTTTCTTTTTTCCAATTCCATTTATTGTAAATCATTTTATTTTCTTTTTTCTTTTTTCATTTCGCCCATGGTGCTCATCATATTGTTTCATGTTTATTTATTTATATACCTGTTATCTAGGGGGGGAAAAAACTCAATTTTAGCATGCAATTATATGCTTTATTTCAGTTGTGTATGGGTTTTAAGATTTTATGCTAACTAGTTTTATAGAGTTATAACATTCAACTTGAAACGGATGACATCAACCCCTATAGATAGATAGATAGATCTACAATGATTAAAGAGACAAATATGAACTCAAATGCCTTTGATTGATGTATTGGAACTTTAGTAACTACCTGCGTTGTTGATTCTTGAATATTATAGGTTCTGTTTTGAGTTTTCTCAAGATATATATTTTTTTATAAAAAAAAAAAGTAATCTTCTGTCGTTCCACTTCCAAATGGTCCTTGCCACAATGTTATACATGGGAGGCAGCTTAGCCTAACAGAAAATGGCTGCTGCTATTGGAGGTCTTTTTTTATGGAGGCCACCTTCCATCATGTTTGAGTACTTTTCAATGAAAACATTTAAAAAGAAAAAAGGCTTTAAGTTGGCTCTGATGAAGGGCAGACAAGGCAGGCAGTGCATGCAACTTTAGAAACCATTCCTTTCTTTCTTACACGAAGAAGTAGAAAGATCAGTCATGGCCTCCAAAATCACTGCCTTCCTTCTCTACAGGAGAGTTAGGGGCCTCCAATACTAAGATGAATCTTAATCACTCTCCTAACAAGGAATCTGACCTGGATGAGAATTTCTGAGCTGTTGATGGATTCCAAACAGAGATGAGGAAGATTTTGATGGTTGACAGACCAGATTATGATGATCCTGTAGGTAACCGTAAGCATGAGCCTCAGAAACCAGGCACTGGAAAGACATAGGACTTGAGATTCCTGTGTTTCCCTTGGGCAAATAATGAGGATGAAAATGGGAACTCTCCTGATAGTCTCCTTGTGAACCAAATAAGACTTCCAAATTGAAAAGTTCTGAATCCTAATGGAAGGTGTAGAATGAATCAACAAGTTGTAGTGCAGTTTTAGAGGCATGATAGTTAAGGCGTCAAACTCTTGTGAGGATGAAAAGTATGGAAGAATAAGCTGATTATACAACAGTGGGACACACAAACTTTAAAGACAGAAAATAGAGGACGCCCACCAAATACCGAAAGAAACGACCCCAATCGTACCAAAAGCTTGAGCCACTTCAAATATGACGTTGTCTTGCTGGAGGGGCAAATTCTGCCAATCTGTCCGCCTGCATCTCATCTCCAGTTAGTTTAAATCATATTTGTATTGGAAGAACTCCCACAACAACAAATAAGACAGTCTAATAACTGAGATCGAGTTTCGTTAGCTGCTGTATTTAGCTTCTCAGAAGAAAGTTTATTGTTTCTATGCATTTGGAAACAAGCAAAGCCATTTGAACTTTTATTCTTTTAATGCCTAGTATTGTAACTGTCTCCTCATTTGCCATGTTTATACTTAAGTGTACTCACCCCAAATCAGCCATGATAATGCTGATATTGTCTTGTGACCCTTTGTCCTACAAGATACAAACATAATATTTGCTTTATTAGAACTGTAAACCAAGTTCTTGTTATTAGAAGCTTTAAACAAATCAAATTGTGCGAGTATTACCAAGGCTGCTTGTGCGAGGGCTTCACAAGCTAACTGAAAATCAATGTAAATGAGAAGTTAGAAGTTGAACCATAACCAAACAGAGACAGCAAGAAATAGAGTGCGTAAAAGAATAAAGAATACAAGTAACATGATGTACACCTGAACATCACCATGCTGACTCAATTGATTCCGAACAAACTTAACCGCATCGGAGCTGATACAACACCCGAAAATGGCACTTATTTAGTCTGTCTAGATCAAAGTTGATGGACAATCTGATAAATCTTTAGGACATAAAGACTGAAGCCTAGAAATGACTGAGATACGAAAAATGACCTATTCATGTAATCCCATAGGCCATCCGATGCTATCAGTACAAACTCGACATTTGATCCGAGGGTAAGCTGAAGAACTTCTGGTGATGCGGTTACCAAATCTCCATTGAACTGTACACTG

Coding sequence (CDS)

ATGGGGAGGGCGGTGGTTTATGTTCTTATATTCGCCGCCTTCGCTGCTTTCTTCGTATTTTCTCCGTCCGATCTCCCCGATCGGAACCGTCAAGAACTAACTCGTCGCCTAGGTTACAAATTCCGAACCCCCACTTTTGATCCTCTTGTGGCAGAATTGGAAAGAATCGCAGAGGAACGTGAATTGGGAAGCCGAGGAAACGTGATTGGAGTGGAACAAACTGCGAAGGCAAAGATTCCCGAGGCTTACAGAGATTCCAACGACGAAGGGAGATTGAACATTTCGTCGAGGTTGGTCGTATTGTTTCCTTTGGTGGACCACTCCCCAAAAGATGGGCTTATCAACTACGAGGAGATGAGCAATTGGATTACTGAGCAAACCATCAACAAATTGAACAGCAGAACGGGGAGAGAGTTAGCGTTCTACGACAAGAACGGAGATGGCGCCATATCTTTCCACGAATATCTGCCGCAGTTTACAGAGGAAGATATTGCTAGAAACGAAACGGGGCATGGACAAGCTGGATGGTGGAAACAGCAATTCACAAATGCTGATGTTGACCCAAATGGATTGCTCAGTTTTGATGAACTGAAAGATTTCCTGCACCCACAAGACAGCAGCAATTACCGAATACAAAACTGGCTGTTGAGCGAGAAAATGAAGCGCATGGACTATGACAGAGATGGAAAACTCAGTTTTGATGAATTCCTCCACCACGCATACGACATATACAAGAGCTATATTGAGTTCGAAACTCAAGGGGACGACGTACCGACCGCGGAAGAGAAATTTGATGAGCTTGATCTTGATGAGGATGAAGTATTGACAGTGGAAGAATTAAGGCCATTATTCCAGTATCTTAATCCTGGAGAACTTTCTCATGCCCAACATTACGCAGCTCATTTGATTAATGAGGCTGATGATAACAAGGATGGGTTCTTAACACTCGAGGAGATGCTTAAACACGAGTACGTATTCTACACCACACTATATGAAAATCAAAATGGCGACTATGAAGACGATTTCCATGATGAACTTTGA

Protein sequence

MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEERELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL
Homology
BLAST of Cp4.1LG09g00260 vs. ExPASy Swiss-Prot
Match: Q66JA6 (45 kDa calcium-binding protein OS=Xenopus laevis OX=8355 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.8e-15
Identity = 78/283 (27.56%), Postives = 134/283 (47.35%), Query Frame = 0

Query: 85  DSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAFY-- 144
           D + E R N   +L  +F  VD + +D  I+  EM  WI E+T              +  
Sbjct: 89  DEDSEPRRN-RRKLAAIFAKVDRN-EDKQISASEMQRWIMEKTEEHFQEAVNENKLHFRA 148

Query: 145 -DKNGDGAISFHEYLPQF----------------TEEDIARNETGHGQAGWWKQQFTNAD 204
            D +GDG +S+ EY  +F                  ED+  +E         K ++  AD
Sbjct: 149 VDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRWFQAD 208

Query: 205 -VDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIY 264
              P+ LL+ +E   FLHP+ S    +  +++ E ++ +D D D KL+  EF+       
Sbjct: 209 NPPPDQLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLPVGTV 268

Query: 265 KSYIEFETQGDDVPTAEEKFDE-LDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLI 324
           ++    +   D V   +++++E +D + D ++T+EEL    +Y++P    +A + A  +I
Sbjct: 269 ENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTMEELE---EYMDPMNEYNALNEAKQMI 328

Query: 325 NEADDNKDGFLTLEEMLKH-EYVFYTTLYENQNGDYEDDFHDE 346
             AD+N+D  L+LEE+LK+ EY   + L      DY  + H+E
Sbjct: 329 AVADENQDHLLSLEEILKYSEYFTGSKLM-----DYARNVHEE 359

BLAST of Cp4.1LG09g00260 vs. ExPASy Swiss-Prot
Match: Q5BKL9 (45 kDa calcium-binding protein OS=Xenopus tropicalis OX=8364 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 4.9e-15
Identity = 78/283 (27.56%), Postives = 134/283 (47.35%), Query Frame = 0

Query: 85  DSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAFY-- 144
           D + E R N   +L  +F  VD + +D  I+  EM  WI E+T              +  
Sbjct: 89  DEDSEPRRN-RRKLAAIFAKVDRN-EDKQISANEMQRWIMEKTEEHFQEAVNENKLHFRA 148

Query: 145 -DKNGDGAISFHEYLPQF----------------TEEDIARNETGHGQAGWWKQQFTNAD 204
            D +GDG +S+ EY  +F                  ED+  +E         K ++  AD
Sbjct: 149 VDPDGDGHVSWDEYKIKFLASKGFNEKEVAEKLKNNEDLKIDEETQEVLDNLKDRWFQAD 208

Query: 205 VDP-NGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIY 264
             P + LL+ +E   FLHP+ S    +  +++ E ++ +D D D KL+  EF+       
Sbjct: 209 NPPADQLLNEEEFLSFLHPEHSQG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLPVGTV 268

Query: 265 KSYIEFETQGDDVPTAEEKFDE-LDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLI 324
           ++    +   D V   +++++E +D + D ++T+EEL    +Y++P    +A + A  +I
Sbjct: 269 ENQQAQDIDDDWVRDRKKEYEEVIDANHDGIVTMEELE---EYMDPMNEYNALNEAKQMI 328

Query: 325 NEADDNKDGFLTLEEMLKH-EYVFYTTLYENQNGDYEDDFHDE 346
             AD+N+D  L+LEE+LK+ EY   + L      DY  + H+E
Sbjct: 329 AVADENQDHHLSLEEILKYSEYFTGSKLM-----DYARNVHEE 359

BLAST of Cp4.1LG09g00260 vs. ExPASy Swiss-Prot
Match: Q7ZUC2 (45 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.9e-14
Identity = 78/283 (27.56%), Postives = 139/283 (49.12%), Query Frame = 0

Query: 85  DSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRT-GRELAF-- 144
           + + E R N   +L+ +F  VD + KD  ++ +EM  WI E+T           +L+F  
Sbjct: 85  EEDSEPRRN-RKKLIEIFTKVDIN-KDRSVSAKEMQRWIMEKTEEHFQEAVRENKLSFRA 144

Query: 145 YDKNGDGAISFHEYLPQF----------------TEEDIARNETGHGQAGWWKQQFTNAD 204
            D +GDG +++ EY  +F                  E++  +E         K ++  AD
Sbjct: 145 VDPDGDGLVTWDEYRVKFLASKGLNEKEVAEKIKNNEELKVDEETQEVLESLKDRWFQAD 204

Query: 205 VDP-NGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIY 264
             P + LL+ +E   FLHP+ S    +  +++ E ++ +D D DGKL+  EF+       
Sbjct: 205 NPPADQLLNEEEFLSFLHPEHSRG--MLRYMVKEIVRDLDQDGDGKLTLAEFISLPMGTV 264

Query: 265 KSYIEFETQGDDVPTAEEKFDE-LDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLI 324
           ++    +   D V   +++F+E +D + D ++T+EEL    +Y++P    +A + A  +I
Sbjct: 265 ENQQAQDIDDDWVRERKKEFEEVIDANHDTIVTMEELE---EYMDPMNEHNALNEAKQMI 324

Query: 325 NEADDNKDGFLTLEEMLKH-EYVFYTTLYENQNGDYEDDFHDE 346
             AD+N++  L LEE+LK+ EY   + L      DY  + H+E
Sbjct: 325 AVADENQNHNLELEEILKYSEYFTGSKLM-----DYARNVHEE 355

BLAST of Cp4.1LG09g00260 vs. ExPASy Swiss-Prot
Match: Q8BP92 (Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.9e-14
Identity = 75/266 (28.20%), Postives = 124/266 (46.62%), Query Frame = 0

Query: 97  RLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYL 156
           RL  +   +D S  DG +   E+S WI     +       ++   YDKN DGA+++ EY 
Sbjct: 68  RLQSIIKKID-SDSDGFLTENELSQWIQMSFKHYAMQEAKQQFVEYDKNSDGAVTWDEYN 127

Query: 157 PQF------TEEDIARNETGHGQAGWW----KQQFTNADVDPNGLLSFDELKDFLHPQDS 216
            Q        +E+ A ++T  G         K++F  A+ D    LS +E   F HP++ 
Sbjct: 128 IQMYDRVIDFDENTALDDTEEGSFRQLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEV 187

Query: 217 SNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIYKSYIEFETQGDD---VPTAEEK 276
               +  +++ E ++  D + DG +S +EFL         Y    T  +D   +   +++
Sbjct: 188 D--YMTEFVIQEALEEHDKNGDGFVSLEEFL-------GDYRRDPTANEDPEWILVEKDR 247

Query: 277 F-DELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLINEADDNKDGFLTLEEMLKH 336
           F ++ D D D  L  +E   L  ++ P     AQ  A HLI+E D N D  L+ EE+L++
Sbjct: 248 FVNDYDKDNDGRLDPQE---LLSWVVPNNQGIAQEEALHLIDEMDLNSDKKLSEEEILEN 307

Query: 337 EYVFYTTLYENQNGDYEDD--FHDEL 347
           + +F T+   +      DD  +HDEL
Sbjct: 308 QDLFLTSEATDYGRQLHDDYFYHDEL 320

BLAST of Cp4.1LG09g00260 vs. ExPASy Swiss-Prot
Match: Q5ZKE5 (45 kDa calcium-binding protein OS=Gallus gallus OX=9031 GN=SDF4 PE=2 SV=2)

HSP 1 Score: 77.8 bits (190), Expect = 2.7e-13
Identity = 77/283 (27.21%), Postives = 132/283 (46.64%), Query Frame = 0

Query: 85  DSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAFY-- 144
           + + E R N   +L+V+F  VD    D  I+ +EM  WI E+T              +  
Sbjct: 85  EEDSEPRKN-RKKLMVIFSKVD-IDNDKKISAKEMQRWIMEKTDEHFQEAVEENKMHFRA 144

Query: 145 -DKNGDGAISFHEYLPQF----------------TEEDIARNETGHGQAGWWKQQFTNAD 204
            D +GDG +S+ EY  +F                  E++  +E         K ++  AD
Sbjct: 145 VDPDGDGHVSWDEYKIKFLASKGLNEKEIAEKIKNNEELKIDEETQEVLDNLKDRWYQAD 204

Query: 205 -VDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIY 264
              P+ LL+ +E   FLHP+ S    +  +++ E ++ +D D D KL+  EF+       
Sbjct: 205 NPPPDMLLNEEEFLSFLHPEHSRG--MLKFMVKEIIRDLDQDGDKKLTLSEFISLPVGTV 264

Query: 265 KSYIEFETQGDDVPTAEEKF-DELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLI 324
           ++    +   D V    ++F D +D + D ++T+EEL    +Y++P    +A + A  +I
Sbjct: 265 ENQQAQDIDDDWVKDRRKEFEDVIDANHDGIVTMEELE---EYMDPMNEYNALNEAKQMI 324

Query: 325 NEADDNKDGFLTLEEMLKH-EYVFYTTLYENQNGDYEDDFHDE 346
             AD+N++  L LEE+LK+ EY   + L      DY  + H+E
Sbjct: 325 AVADENQNHHLELEEILKYSEYFTGSKLM-----DYARNVHEE 355

BLAST of Cp4.1LG09g00260 vs. NCBI nr
Match: XP_023541445.1 (reticulocalbin-2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706 bits (1823), Expect = 6.55e-257
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS
Sbjct: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA
Sbjct: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL
Sbjct: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Cp4.1LG09g00260 vs. NCBI nr
Match: XP_022945265.1 (reticulocalbin-2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 696 bits (1796), Expect = 8.56e-253
Identity = 340/346 (98.27%), Postives = 342/346 (98.84%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLI AAFAAFFVFSPS LPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGN IGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSP DGLINYEEMS
Sbjct: 61  ELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQT+NKLN+RTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA
Sbjct: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL
Sbjct: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Cp4.1LG09g00260 vs. NCBI nr
Match: KAG6574244.1 (45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 694 bits (1792), Expect = 3.49e-252
Identity = 339/346 (97.98%), Postives = 342/346 (98.84%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLI AAFAAFFVFSPS LPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGN IGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSP DGLINYEEMS
Sbjct: 61  ELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQT+NKLN+RTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQH+AA
Sbjct: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHFAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL
Sbjct: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Cp4.1LG09g00260 vs. NCBI nr
Match: KAG7013311.1 (45 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 692 bits (1787), Expect = 2.02e-251
Identity = 338/346 (97.69%), Postives = 341/346 (98.55%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLI AAFAAFFVFSPS LPDRNRQELTRRLGY+FRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYRFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGN IGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSP DGLINYEEMS
Sbjct: 61  ELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQT+NKLN+RTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA
Sbjct: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           HLI EADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL
Sbjct: 301 HLIKEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Cp4.1LG09g00260 vs. NCBI nr
Match: XP_022968155.1 (calumenin-B isoform X1 [Cucurbita maxima])

HSP 1 Score: 679 bits (1751), Expect = 6.20e-246
Identity = 334/346 (96.53%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGN IGVE+TAKAKIPEAYR SNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS
Sbjct: 61  ELGSRGNAIGVERTAKAKIPEAYRYSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQTI+KLNSRT RELAFYDKNGDGAISFHEYLPQF+EEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTIDKLNSRTRRELAFYDKNGDGAISFHEYLPQFSEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLL+FDELKDFLHPQDSSNYRIQNWLLSEKMKRMDY RDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLNFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYGRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           Y+IYKSYIEFETQG DVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA
Sbjct: 241 YNIYKSYIEFETQGVDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           +LINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDD HDEL
Sbjct: 301 YLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDSHDEL 346

BLAST of Cp4.1LG09g00260 vs. ExPASy TrEMBL
Match: A0A6J1G0G2 (reticulocalbin-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 SV=1)

HSP 1 Score: 696 bits (1796), Expect = 4.15e-253
Identity = 340/346 (98.27%), Postives = 342/346 (98.84%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLI AAFAAFFVFSPS LPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLILAAFAAFFVFSPSHLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGN IGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSP DGLINYEEMS
Sbjct: 61  ELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPTDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQT+NKLN+RTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTVNKLNNRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA
Sbjct: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL
Sbjct: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346

BLAST of Cp4.1LG09g00260 vs. ExPASy TrEMBL
Match: A0A6J1HWE3 (calumenin-B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467475 PE=4 SV=1)

HSP 1 Score: 679 bits (1751), Expect = 3.00e-246
Identity = 334/346 (96.53%), Postives = 340/346 (98.27%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER
Sbjct: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60

Query: 61  ELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120
           ELGSRGN IGVE+TAKAKIPEAYR SNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS
Sbjct: 61  ELGSRGNAIGVERTAKAKIPEAYRYSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMS 120

Query: 121 NWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQ 180
           NWITEQTI+KLNSRT RELAFYDKNGDGAISFHEYLPQF+EEDIARNETGHGQAGWWKQQ
Sbjct: 121 NWITEQTIDKLNSRTRRELAFYDKNGDGAISFHEYLPQFSEEDIARNETGHGQAGWWKQQ 180

Query: 181 FTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHA 240
           FTNADVDPNGLL+FDELKDFLHPQDSSNYRIQNWLLSEKMKRMDY RDGKLSFDEFLHHA
Sbjct: 181 FTNADVDPNGLLNFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYGRDGKLSFDEFLHHA 240

Query: 241 YDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300
           Y+IYKSYIEFETQG DVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA
Sbjct: 241 YNIYKSYIEFETQGVDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 300

Query: 301 HLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           +LINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDD HDEL
Sbjct: 301 YLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDSHDEL 346

BLAST of Cp4.1LG09g00260 vs. ExPASy TrEMBL
Match: A0A6J1G0D5 (45 kDa calcium-binding protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 SV=1)

HSP 1 Score: 602 bits (1552), Expect = 2.09e-216
Identity = 297/312 (95.19%), Postives = 299/312 (95.83%), Query Frame = 0

Query: 35  RRLGYKFRTPTFDPLVAELERIAEERELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNI 94
           R  GY    P  DP   ELERIAEERELGSRGN IGVEQTAKAKIPEAYRDSNDEGRLNI
Sbjct: 9   RHRGYCIGIPP-DP---ELERIAEERELGSRGNAIGVEQTAKAKIPEAYRDSNDEGRLNI 68

Query: 95  SSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAFYDKNGDGAISFHE 154
           SSRLVVLFPLVDHSP DGLINYEEMSNWITEQT+NKLN+RTGRELAFYDKNGDGAISFHE
Sbjct: 69  SSRLVVLFPLVDHSPTDGLINYEEMSNWITEQTVNKLNNRTGRELAFYDKNGDGAISFHE 128

Query: 155 YLPQFTEEDIARNETGHGQAGWWKQQFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNW 214
           YLPQFTEEDIARNETGHGQAGWWKQQFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNW
Sbjct: 129 YLPQFTEEDIARNETGHGQAGWWKQQFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNW 188

Query: 215 LLSEKMKRMDYDRDGKLSFDEFLHHAYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEV 274
           LLSEKMKRMDYDRDGKLSFDEFLHHAYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEV
Sbjct: 189 LLSEKMKRMDYDRDGKLSFDEFLHHAYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEV 248

Query: 275 LTVEELRPLFQYLNPGELSHAQHYAAHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQ 334
           LTVEELRPLFQYLNPGELSHAQHYAAHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQ
Sbjct: 249 LTVEELRPLFQYLNPGELSHAQHYAAHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQ 308

Query: 335 NGDYEDDFHDEL 346
           NGDYEDDFHDEL
Sbjct: 309 NGDYEDDFHDEL 316

BLAST of Cp4.1LG09g00260 vs. ExPASy TrEMBL
Match: A0A6J1HU32 (45 kDa calcium-binding protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467475 PE=4 SV=1)

HSP 1 Score: 578 bits (1490), Expect = 5.85e-207
Identity = 283/295 (95.93%), Postives = 289/295 (97.97%), Query Frame = 0

Query: 52  ELERIAEERELGSRGNVIGVEQTAKAKIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKD 111
           ELERIAEERELGSRGN IGVE+TAKAKIPEAYR SNDEGRLNISSRLVVLFPLVDHSPKD
Sbjct: 22  ELERIAEERELGSRGNAIGVERTAKAKIPEAYRYSNDEGRLNISSRLVVLFPLVDHSPKD 81

Query: 112 GLINYEEMSNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGH 171
           GLINYEEMSNWITEQTI+KLNSRT RELAFYDKNGDGAISFHEYLPQF+EEDIARNETGH
Sbjct: 82  GLINYEEMSNWITEQTIDKLNSRTRRELAFYDKNGDGAISFHEYLPQFSEEDIARNETGH 141

Query: 172 GQAGWWKQQFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKL 231
           GQAGWWKQQFTNADVDPNGLL+FDELKDFLHPQDSSNYRIQNWLLSEKMKRMDY RDGKL
Sbjct: 142 GQAGWWKQQFTNADVDPNGLLNFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYGRDGKL 201

Query: 232 SFDEFLHHAYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGE 291
           SFDEFLHHAY+IYKSYIEFETQG DVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGE
Sbjct: 202 SFDEFLHHAYNIYKSYIEFETQGVDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGE 261

Query: 292 LSHAQHYAAHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           LSHAQHYAA+LINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDD HDEL
Sbjct: 262 LSHAQHYAAYLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDSHDEL 316

BLAST of Cp4.1LG09g00260 vs. ExPASy TrEMBL
Match: A0A6J1CHQ2 (reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1)

HSP 1 Score: 551 bits (1421), Expect = 5.92e-196
Identity = 262/347 (75.50%), Postives = 302/347 (87.03%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQELTRRLGYKFRTPTFDPLVAELERIAEER 60
           MGRAVVY+ +  AFA FFVF PS+ PDRN QELTRRLGYK R PTFDPLV E+ER AEE 
Sbjct: 1   MGRAVVYLFLITAFAFFFVFYPSNSPDRNHQELTRRLGYKLRAPTFDPLVEEMERYAEEH 60

Query: 61  ELGSRGNVIGVEQTAKA-KIPEAYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEM 120
             G   N I  ++T  A ++P+AY+D  DEGRL IS RL+VLFPL+DHSPKDG+I+YEE+
Sbjct: 61  GSGRGRNAISGQRTTTANQVPDAYKDYYDEGRLKISLRLLVLFPLLDHSPKDGVISYEEL 120

Query: 121 SNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQ 180
           S+WI EQ + +LN RT +EL F+DKNGDGAISF EYLPQFTEEDIA+  T HG+AGWWK+
Sbjct: 121 SDWIIEQAVQRLNYRTRKELDFHDKNGDGAISFREYLPQFTEEDIAKRGTAHGEAGWWKR 180

Query: 181 QFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHH 240
           QFTNAD DPNGLL+F+ELKDFLHP+DSSNYRIQNWLL EKMKRMDYDRDGKL+FDEFLHH
Sbjct: 181 QFTNADTDPNGLLNFEELKDFLHPEDSSNYRIQNWLLREKMKRMDYDRDGKLNFDEFLHH 240

Query: 241 AYDIYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYA 300
            +DIYKSYIEFETQGDDVPTAEEKFDELDLDEDE+L+VEELRPLFQYL+PGELS+AQHY 
Sbjct: 241 VFDIYKSYIEFETQGDDVPTAEEKFDELDLDEDELLSVEELRPLFQYLHPGELSYAQHYT 300

Query: 301 AHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 346
           +HLI EADDNKDG+L+L+EML HEYVFY ++YE +NGDYEDD+HDEL
Sbjct: 301 SHLIYEADDNKDGYLSLDEMLNHEYVFYNSVYEGRNGDYEDDYHDEL 347

BLAST of Cp4.1LG09g00260 vs. TAIR 10
Match: AT4G27790.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.6e-80
Identity = 149/350 (42.57%), Postives = 221/350 (63.14%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFVFSPSDLPDRNRQE--LTRRLGYKFRTPTFDPLVAELERIAE 60
           M + VVY ++ A      + +          E  +TRR+G +   P FDPLV  +ER++ 
Sbjct: 1   MAKVVVYTILTATIIFLILLAHKKQNQTQSIEGLITRRIGRRLEMPVFDPLVTRIERLSH 60

Query: 61  ERELGSRGNVIGVEQTAKAKIPEAYRD-SNDEGRLNISSRLVVLFPLVDHSPKDGLINYE 120
           E+E G++       + AK +  + + +    E RLN + R+  LFPL+D SP+DG ++ +
Sbjct: 61  EKEAGTK-----TVEAAKEEKDDMFEEYFAQERRLNTTMRIKFLFPLLDASPRDGFVSLK 120

Query: 121 EMSNWITEQTINKLNSRTGRELAFYDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWW 180
           E+  W+ +QT + +  RT +EL   DK+ DG I+F EYLPQF+++DI +NE GHG+AGWW
Sbjct: 121 ELQTWMMQQTEDNMVYRTAKELELQDKDKDGVITFEEYLPQFSKQDIEKNEKGHGEAGWW 180

Query: 181 KQQFTNADVDPNGLLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFL 240
            +QF N+D D NG L  +E  +FLHP+DS N   Q W+L E+M  MD + DGKL + EF+
Sbjct: 181 MEQFKNSDFDHNGSLDIEEFNNFLHPEDSRNGDTQRWVLKERMTGMDTNGDGKLEYKEFV 240

Query: 241 HHAYDIYKSYIEFETQGDD-VPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQ 300
            +AY++YK + +FE + D+ VPT +  F E+D D+D  L  +ELRP+ QYL PGE+S+A+
Sbjct: 241 KNAYEMYKEFAKFEKEEDENVPTPQLLFAEMDRDKDRFLVADELRPILQYLQPGEMSYAK 300

Query: 301 HYAAHLINEADDNKDGFLTLEEMLKHEYVFYTTLYENQNGDYEDDFHDEL 347
            Y+  L +EAD++KDG L+LEEML HE VFY  ++     D +   HDEL
Sbjct: 301 FYSTFLCHEADEDKDGKLSLEEMLHHEDVFYKAVHHEDLDDEDYFDHDEL 345

BLAST of Cp4.1LG09g00260 vs. TAIR 10
Match: AT5G08580.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 200.7 bits (509), Expect = 2.0e-51
Identity = 129/391 (32.99%), Postives = 202/391 (51.66%), Query Frame = 0

Query: 1   MGRAVVYVLIFAAFAAFFV--FSPSDLPDRN--------RQELTRRLGYKFRTPT----- 60
           M +A V + I       F+  +SP    D +           L  R  + F+ PT     
Sbjct: 1   MSKASVILYITVGILVLFLVSYSPKKKGDHDHHHGGHNQHHRLKLRSSFNFK-PTRHDPV 60

Query: 61  -FDPLVAELERIAEEREL----------------------GSRGNVIGVE-QTAKAKIPE 120
            FDPLVA++ER  E++E                       G   +  G E Q    +  +
Sbjct: 61  PFDPLVADMERRREDKEWERQYIEHSHPELVSHSQKETTGGGHEHAPGHESQPEWEEFMD 120

Query: 121 AYRDSNDEGRLNISSRLVVLFPLVDHSPKDGLINYEEMSNWITEQTINKLNSRTGRELAF 180
           A    NDE + N++ RL++LFP +D SP DG +   E++ W  + +  ++  RT R+L  
Sbjct: 121 AEDYLNDEEKFNVTDRLILLFPKIDVSPADGFMTESELTEWTMQSSAKEVVHRTQRDLDV 180

Query: 181 YDKNGDGAISFHEYLPQFTEEDIARNETGHGQAGWWKQQFTNA-DVDPNGLLSFDELKDF 240
           +D+N DG ISF EY P         N  G+   GWWK++  NA D + +GLL+  E  DF
Sbjct: 181 HDRNKDGFISFSEYEPPSWVRKSDNNSFGY-DMGWWKEEHFNASDANGDGLLNLTEFNDF 240

Query: 241 LHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLHHAYDIYKSYIEFETQG----DD 300
           LHP D+ N ++  WL  E+++  D D+DGK+SF+EF H  +D  ++Y E          D
Sbjct: 241 LHPADTKNPKLLLWLCKEEVRERDSDKDGKISFEEFFHGLFDTVRNYEEDNHNSTHPYHD 300

Query: 301 VP--TAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAAHLINEADDNKDGFL 346
           +P   A++ F +LD ++D  L+  EL P+   ++P E  +A+  A ++I++AD +KD  L
Sbjct: 301 LPEGPAKQLFSQLDKNDDGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRL 360

BLAST of Cp4.1LG09g00260 vs. TAIR 10
Match: AT2G41100.1 (Calcium-binding EF hand family protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.6e-07
Identity = 51/200 (25.50%), Postives = 89/200 (44.50%), Query Frame = 0

Query: 143 DKNGDGAISFHEYL------------PQFTEEDIARNETGHGQAGWWKQQFTNADVDPNG 202
           D +GDG I F E+L            P+ T++ +A   T   Q   +++ F   D + +G
Sbjct: 57  DLDGDGTIDFPEFLCVMAKNQGHDQAPRHTKKTMADKLT-DDQITEYRESFRLFDKNGDG 116

Query: 203 LLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLH-----HAYD--- 262
            ++  EL+  +     +  +     L + M  +D D DG + F EFL+       +D   
Sbjct: 117 SITKKELRTVMFSLGKNRTKAD---LQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQAP 176

Query: 263 --IYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 321
               K+ ++++   D +    E F   D + D  +TV ELR   + L  GE +  +    
Sbjct: 177 RHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTMRSL--GE-TQTKAELQ 236

BLAST of Cp4.1LG09g00260 vs. TAIR 10
Match: AT2G41100.3 (Calcium-binding EF hand family protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.6e-07
Identity = 51/200 (25.50%), Postives = 89/200 (44.50%), Query Frame = 0

Query: 143 DKNGDGAISFHEYL------------PQFTEEDIARNETGHGQAGWWKQQFTNADVDPNG 202
           D +GDG I F E+L            P+ T++ +A   T   Q   +++ F   D + +G
Sbjct: 22  DLDGDGTIDFPEFLCVMAKNQGHDQAPRHTKKTMADKLT-DDQITEYRESFRLFDKNGDG 81

Query: 203 LLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLH-----HAYD--- 262
            ++  EL+  +     +  +     L + M  +D D DG + F EFL+       +D   
Sbjct: 82  SITKKELRTVMFSLGKNRTKAD---LQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQAP 141

Query: 263 --IYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 321
               K+ ++++   D +    E F   D + D  +TV ELR   + L  GE +  +    
Sbjct: 142 RHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTMRSL--GE-TQTKAELQ 201

BLAST of Cp4.1LG09g00260 vs. TAIR 10
Match: AT2G41100.4 (Calcium-binding EF hand family protein )

HSP 1 Score: 52.4 bits (124), Expect = 8.6e-07
Identity = 51/200 (25.50%), Postives = 89/200 (44.50%), Query Frame = 0

Query: 143 DKNGDGAISFHEYL------------PQFTEEDIARNETGHGQAGWWKQQFTNADVDPNG 202
           D +GDG I F E+L            P+ T++ +A   T   Q   +++ F   D + +G
Sbjct: 57  DLDGDGTIDFPEFLCVMAKNQGHDQAPRHTKKTMADKLT-DDQITEYRESFRLFDKNGDG 116

Query: 203 LLSFDELKDFLHPQDSSNYRIQNWLLSEKMKRMDYDRDGKLSFDEFLH-----HAYD--- 262
            ++  EL+  +     +  +     L + M  +D D DG + F EFL+       +D   
Sbjct: 117 SITKKELRTVMFSLGKNRTKAD---LQDMMNEVDLDGDGTIDFPEFLYLMAKNQGHDQAP 176

Query: 263 --IYKSYIEFETQGDDVPTAEEKFDELDLDEDEVLTVEELRPLFQYLNPGELSHAQHYAA 321
               K+ ++++   D +    E F   D + D  +TV ELR   + L  GE +  +    
Sbjct: 177 RHTKKTMVDYQLTDDQILEFREAFRVFDKNGDGYITVNELRTTMRSL--GE-TQTKAELQ 236

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q66JA63.8e-1527.5645 kDa calcium-binding protein OS=Xenopus laevis OX=8355 GN=sdf4 PE=2 SV=1[more]
Q5BKL94.9e-1527.5645 kDa calcium-binding protein OS=Xenopus tropicalis OX=8364 GN=sdf4 PE=2 SV=1[more]
Q7ZUC21.9e-1427.5645 kDa calcium-binding protein OS=Danio rerio OX=7955 GN=sdf4 PE=2 SV=1[more]
Q8BP921.9e-1428.20Reticulocalbin-2 OS=Mus musculus OX=10090 GN=Rcn2 PE=1 SV=1[more]
Q5ZKE52.7e-1327.2145 kDa calcium-binding protein OS=Gallus gallus OX=9031 GN=SDF4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_023541445.16.55e-257100.00reticulocalbin-2 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022945265.18.56e-25398.27reticulocalbin-2 isoform X1 [Cucurbita moschata][more]
KAG6574244.13.49e-25297.9845 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. sororia][more]
KAG7013311.12.02e-25197.6945 kDa calcium-binding protein, partial [Cucurbita argyrosperma subsp. argyrospe... [more]
XP_022968155.16.20e-24696.53calumenin-B isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1G0G24.15e-25398.27reticulocalbin-2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449565 PE=4 S... [more]
A0A6J1HWE33.00e-24696.53calumenin-B isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467475 PE=4 SV=1[more]
A0A6J1G0D52.09e-21695.1945 kDa calcium-binding protein isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1HU325.85e-20795.9345 kDa calcium-binding protein isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1CHQ25.92e-19675.50reticulocalbin-2 OS=Momordica charantia OX=3673 GN=LOC111011736 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT4G27790.12.6e-8042.57Calcium-binding EF hand family protein [more]
AT5G08580.12.0e-5132.99Calcium-binding EF hand family protein [more]
AT2G41100.18.6e-0725.50Calcium-binding EF hand family protein [more]
AT2G41100.38.6e-0725.50Calcium-binding EF hand family protein [more]
AT2G41100.48.6e-0725.50Calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 215..243
e-value: 0.35
score: 19.9
coord: 298..326
e-value: 37.0
score: 5.1
coord: 134..162
e-value: 49.0
score: 4.1
coord: 176..204
e-value: 1.8
score: 15.8
coord: 259..287
e-value: 33.0
score: 5.5
IPR002048EF-hand domainPFAMPF13202EF-hand_5coord: 142..154
e-value: 0.013
score: 15.0
coord: 220..236
e-value: 0.0016
score: 17.9
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 261..321
e-value: 4.6E-8
score: 33.4
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 255..290
score: 9.520128
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 220..246
score: 8.599581
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 172..207
score: 8.320626
NoneNo IPR availableGENE3D1.10.238.10coord: 158..243
e-value: 3.5E-11
score: 45.2
NoneNo IPR availableGENE3D1.10.238.10coord: 94..157
e-value: 1.7E-5
score: 26.9
NoneNo IPR availableGENE3D1.10.238.10coord: 244..325
e-value: 8.0E-11
score: 44.1
NoneNo IPR availablePANTHERPTHR10827RETICULOCALBINcoord: 1..346
NoneNo IPR availablePANTHERPTHR10827:SF77CALCIUM-BINDING EF HAND FAMILY PROTEINcoord: 1..346
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 143..155
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 224..236
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 307..319
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 185..197
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 96..157
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 174..337

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g00260.1Cp4.1LG09g00260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005509 calcium ion binding