Cp4.1LG08g13970 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG08g13970 vs. ExPASy Swiss-Prot
Match: Q7XA42 (Putative disease resistance protein RGA1 OS=Solanum bulbocastanum OX=147425 GN=RGA1 PE=2 SV=2)

HSP 1 Score: 201.4 bits (511), Expect = 7.5e-50
Identity = 160/507 (31.56%), Postives = 244/507 (48.13%), Query Frame = 0

Query: 1113 LQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNLETLILQDCSQLRELPKDIKKCV 1172
            L +LP    +   LRYL L GN  I+ LP  +  L NL+TL L  C  L  LPK   K  
Sbjct: 537  LNQLPSSIGDLVHLRYLDLSGNFRIRNLPKRLCKLQNLQTLDLHYCDSLSCLPKQTSKLG 596

Query: 1173 NLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGTLTIKSL 1232
            +L++L L GC +LT  P  +G LT L++++ FV+ K  G  L EL  LN L G+++I  L
Sbjct: 597  SLRNLLLDGC-SLTSTPPRIGLLTCLKSLSCFVIGKRKGHQLGELKNLN-LYGSISITKL 656

Query: 1233 EVC--TSDVLENTLRLKLGVRELRLVWNNSVDEYIYIVLDDEGKGVLQCLQLHSKVRTIY 1292
            +     +D  E  L  K  +  L L W+    ++ Y   D E   VL+ L+ HS ++ + 
Sbjct: 657  DRVKKDTDAKEANLSAKANLHSLCLSWDLD-GKHRY---DSE---VLEALKPHSNLKYLE 716

Query: 1293 ICKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQFDQFPFLKHLGLKS-LPNIEYID 1352
            I  +  + L DW++ + L ++VS+ +  C     LP F + P L+ L L +   ++EY++
Sbjct: 717  INGFGGIRLPDWMNQSVLKNVVSIRIRGCENCSCLPPFGELPCLESLELHTGSADVEYVE 776

Query: 1353 NNEYPSSSMFFPSLEELSIVDMHNLKGWWKGETSSESSPNNASFPITMSCLCELEIDACP 1412
            +N +P     FPSL +L I D  NLKG  K E   +       FP+    L E+    CP
Sbjct: 777  DNVHPGR---FPSLRKLVIWDFSNLKGLLKMEGEKQ-------FPV----LEEMTFYWCP 836

Query: 1413 HLASIPWHAYLKSLYLREISSQLLNVVIERTTNNCVEKC-STTSVSLSKIDDLEFLPKGL 1472
             +  IP  + +K+L +         +V + T    +    + TS+ +S   +   LP+ +
Sbjct: 837  -MFVIPTLSSVKTLKV---------IVTDATVLRSISNLRALTSLDISDNVEATSLPEEM 896

Query: 1473 FYHFTNLEYLEIKDCKNLQMSSALVQHPINDVLWKEFRSLCYLSLEAIPKLEYLPNGMKH 1532
            F    NL+YL+I   +N                                 L+ LP  +  
Sbjct: 897  FKSLANLKYLKISFFRN---------------------------------LKELPTSLAS 956

Query: 1533 VTTLERIDIISCPNLITLP-EWIGNFTSLSELMIIVCPKLTSLSERIHHLPSLQDLYIWD 1592
            +  L+ +    C  L +LP E +   TSL+EL +  C  L  L E + HL +L  L I  
Sbjct: 957  LNALKSLKFEFCDALESLPEEGVKGLTSLTELSVSNCMMLKCLPEGLQHLTALTTLTITQ 977

Query: 1593 CPKLKERYERGKGEDWQKISHIPRVTI 1615
            CP + +R ERG GEDW KI+HIP +T+
Sbjct: 1017 CPIVFKRCERGIGEDWHKIAHIPYLTL 977

HSP 2 Score: 64.7 bits (156), Expect = 1.1e-08
Identity = 43/157 (27.39%), Postives = 77/157 (49.04%), Query Frame = 0

Query: 29  LITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEA 88
           ++  L S    E  L  G QDE  +L+   S ++AVL DA+EKQ  +  ++NW+ K+  A
Sbjct: 9   VLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNDKPLENWLQKLNAA 68

Query: 89  FYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMSHKIKDIRDNLKA 148
            Y+ +D++DE+  +  R       + G          P  I FR ++  ++  +   L A
Sbjct: 69  TYEVDDILDEYKTKATR---FLQSEYGRYH-------PKVIPFRHKVGKRMDQVMKKLNA 128

Query: 149 IDADKNQFNFSERMINTRDDELRKRRETSSFICDDEV 186
           I  ++ +F+  E++I    +     RET S + + +V
Sbjct: 129 IAEERKKFHLQEKII----ERQAATRETGSVLTEPQV 151

BLAST of Cp4.1LG08g13970 vs. ExPASy Swiss-Prot
Match: Q7XBQ9 (Disease resistance protein RGA2 OS=Solanum bulbocastanum OX=147425 GN=RGA2 PE=1 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 2.9e-49
Identity = 161/515 (31.26%), Postives = 239/515 (46.41%), Query Frame = 0

Query: 1109 GCECLQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNLETLILQDCSQLRELPKDI 1168
            G     +LP    +   LRYL+L G+  ++ LP  +  L NL+TL LQ C++L  LPK+ 
Sbjct: 534  GDSTFNKLPSSIGDLVHLRYLNLYGS-GMRSLPKQLCKLQNLQTLDLQYCTKLCCLPKET 593

Query: 1169 KKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGTLT 1228
             K  +L++L L G  +LT MP  +G LT L+T+  FV+ +  G  L EL  LN L G++ 
Sbjct: 594  SKLGSLRNLLLDGSQSLTCMPPRIGSLTCLKTLGQFVVGRKKGYQLGELGNLN-LYGSIK 653

Query: 1229 IKSLEVCTS--DVLENTLRLKLGVRELRLVWNNSVDEYIYIVLDDEGKGVLQCLQLHSKV 1288
            I  LE   +  D  E  L  K  +  L + WNN    +IY   + E   VL+ L+ HS +
Sbjct: 654  ISHLERVKNDKDAKEANLSAKGNLHSLSMSWNN-FGPHIY---ESEEVKVLEALKPHSNL 713

Query: 1289 RTIYICKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQFDQFPFLKHLGLK-SLPNI 1348
             ++ I  +  + L +W++ + L ++VS+ +S+      LP F   P L+ L L     ++
Sbjct: 714  TSLKIYGFRGIHLPEWMNHSVLKNIVSILISNFRNCSCLPPFGDLPCLESLELHWGSADV 773

Query: 1349 EYIDNNEYPSSSMF-----FPSLEELSIVDMHNLKGWWKGETSSESSPNNASFPITMSCL 1408
            EY++  +    S F     FPSL +L I D  +LKG  K E   +       FP+    L
Sbjct: 774  EYVEEVDIDVHSGFPTRIRFPSLRKLDIWDFGSLKGLLKKEGEEQ-------FPV----L 833

Query: 1409 CELEIDACPHLASIPWHAYLKSLYLREISSQLLNVVIERTTNNCVEKCSTTSVSLSKIDD 1468
             E+ I  CP L          S  LR ++S  +          C  K +T+         
Sbjct: 834  EEMIIHECPFLT--------LSSNLRALTSLRI----------CYNKVATS--------- 893

Query: 1469 LEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPINDVLWKEFRSLCYLSLEAIPKLE 1528
                P+ +F +  NL+YL I  C N                                 L+
Sbjct: 894  ---FPEEMFKNLANLKYLTISRCNN---------------------------------LK 953

Query: 1529 YLPNGMKHVTTLERIDIISCPNLITLP-EWIGNFTSLSELMIIVCPKLTSLSERIHHLPS 1588
             LP  +  +  L+ + I  C  L +LP E +   +SL+EL +  C  L  L E + HL +
Sbjct: 954  ELPTSLASLNALKSLKIQLCCALESLPEEGLEGLSSLTELFVEHCNMLKCLPEGLQHLTT 968

Query: 1589 LQDLYIWDCPKLKERYERGKGEDWQKISHIPRVTI 1615
            L  L I  CP+L +R E+G GEDW KISHIP V I
Sbjct: 1014 LTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNI 968

HSP 2 Score: 71.2 bits (173), Expect = 1.2e-10
Identity = 46/157 (29.30%), Postives = 79/157 (50.32%), Query Frame = 0

Query: 29  LITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEA 88
           L+  L S    E  L  G QDE  +L+   S ++AVL DA+EKQ  N  ++NW+ K+  A
Sbjct: 9   LLDNLTSFLKGELVLLFGFQDEFQRLSSMFSTIQAVLEDAQEKQLNNKPLENWLQKLNAA 68

Query: 89  FYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMSHKIKDIRDNLKA 148
            Y+ +D++DE+  +  R    +  + G          P  I FR ++  ++  +   LKA
Sbjct: 69  TYEVDDILDEYKTKATR---FSQSEYGRYH-------PKVIPFRHKVGKRMDQVMKKLKA 128

Query: 149 IDADKNQFNFSERMINTRDDELRKRRETSSFICDDEV 186
           I  ++  F+  E+++    +    RRET S + + +V
Sbjct: 129 IAEERKNFHLHEKIV----ERQAVRRETGSVLTEPQV 151

BLAST of Cp4.1LG08g13970 vs. ExPASy Swiss-Prot
Match: Q7XA40 (Putative disease resistance protein RGA3 OS=Solanum bulbocastanum OX=147425 GN=RGA3 PE=2 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 8.3e-49
Identity = 164/528 (31.06%), Postives = 250/528 (47.35%), Query Frame = 0

Query: 1091 FLPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNL 1150
            + P        L  L L   E  ++LP    +   LRYL L GNK I  LP  +  L NL
Sbjct: 526  YSPSLFKRFVSLRVLNLSNSE-FEQLPSSVGDLVHLRYLDLSGNK-ICSLPKRLCKLQNL 585

Query: 1151 ETLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDI 1210
            +TL L +C  L  LPK   K  +L++L L  C  LT MP  +G LT L+T+  FV+ +  
Sbjct: 586  QTLDLYNCQSLSCLPKQTSKLCSLRNLVLDHC-PLTSMPPRIGLLTCLKTLGYFVVGERK 645

Query: 1211 GCDLSELNRLNKLKGTLTIKSLEVCTSDV--LENTLRLKLGVRELRLVWNNSVDEYIYIV 1270
            G  L EL  LN L+G ++I  LE   +D+   E  L  K  +  L + W+   + Y    
Sbjct: 646  GYQLGELRNLN-LRGAISITHLERVKNDMEAKEANLSAKANLHSLSMSWDRP-NRY---- 705

Query: 1271 LDDEGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQF 1330
             + E   VL+ L+ H  ++ + I  +    L DW++ + L ++VS+ +S C     LP F
Sbjct: 706  -ESEEVKVLEALKPHPNLKYLEIIDFCGFCLPDWMNHSVLKNVVSILISGCENCSCLPPF 765

Query: 1331 DQFPFLKHLGLK-SLPNIEYIDNNEYPSSSMFFPSLEELSIVDMHNLKGWWKGETSSESS 1390
             + P L+ L L+     +EY++++ + +    FPSL +L I    NLKG  + + + +  
Sbjct: 766  GELPCLESLELQDGSVEVEYVEDSGFLTRRR-FPSLRKLHIGGFCNLKGLQRMKGAEQ-- 825

Query: 1391 PNNASFPITMSCLCELEIDACPHLASIPWHAYLKSLYLREISSQLLNVVIERTTNNCVEK 1450
                 FP+    L E++I  CP +   P  + +K L   EI  +                
Sbjct: 826  -----FPV----LEEMKISDCP-MFVFPTLSSVKKL---EIWGE---------------- 885

Query: 1451 CSTTSVSLSKIDDLEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPINDVLWKEFRS 1510
                +  LS I +L  L               +K   N  ++S L      + ++K   +
Sbjct: 886  --ADAGGLSSISNLSTL-------------TSLKIFSNHTVTSLL------EEMFKNLEN 945

Query: 1511 LCYLSLEAIPKLEYLPNGMKHVTTLERIDIISCPNLITLP-EWIGNFTSLSELMIIVCPK 1570
            L YLS+  +  L+ LP  +  +  L+ +DI  C  L +LP E +   +SL+EL +  C  
Sbjct: 946  LIYLSVSFLENLKELPTSLASLNNLKCLDIRYCYALESLPEEGLEGLSSLTELFVEHCNM 990

Query: 1571 LTSLSERIHHLPSLQDLYIWDCPKLKERYERGKGEDWQKISHIPRVTI 1615
            L  L E + HL +L  L I  CP+L +R E+G GEDW KISHIP V I
Sbjct: 1006 LKCLPEGLQHLTTLTSLKIRGCPQLIKRCEKGIGEDWHKISHIPNVNI 990

HSP 2 Score: 62.8 bits (151), Expect = 4.2e-08
Identity = 42/152 (27.63%), Postives = 75/152 (49.34%), Query Frame = 0

Query: 40  ECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLIDEF 99
           E  L  G + E  KL+   S ++AVL DA+EKQ     +KNW+ K+  A Y+ +D++D+ 
Sbjct: 20  ELGLVFGFEKEFKKLSSMFSMIQAVLEDAQEKQLKYKAIKNWLQKLNVAAYEVDDILDDC 79

Query: 100 AYETLR-RQVIANDKRGAKEVRQFFSLPHRIAFRFQMSHKIKDIRDNLKAIDADKNQFNF 159
             E  R +Q +                P  I F +++  ++K++ + L AI  ++  F+ 
Sbjct: 80  KTEAARFKQAVLGRYH-----------PRTITFCYKVGKRMKEMMEKLDAIAEERRNFHL 139

Query: 160 SERMINTRDDELRKRRETSSFICDDEVEDCQK 191
            ER+I    +    RR+T   + + +V   +K
Sbjct: 140 DERII----ERQAARRQTGFVLTEPKVYGREK 156

BLAST of Cp4.1LG08g13970 vs. ExPASy Swiss-Prot
Match: Q7XA39 (Putative disease resistance protein RGA4 OS=Solanum bulbocastanum OX=147425 GN=RGA4 PE=2 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 3.9e-46
Identity = 147/513 (28.65%), Postives = 238/513 (46.39%), Query Frame = 0

Query: 1113 LQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNLETLILQDCSQLRELPKDIKKCV 1172
            L++LP    +   LRYL L  N N + LP+ +  L NL+TL + +C  L  LPK   K  
Sbjct: 539  LEQLPSSIGDLLHLRYLDLSCN-NFRSLPERLCKLQNLQTLDVHNCYSLNCLPKQTSKLS 598

Query: 1173 NLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGTLTIKSL 1232
            +L+HL + GC  LT  P  +G LT L+T+  F++    G  L EL  LN L G+++I  L
Sbjct: 599  SLRHLVVDGC-PLTSTPPRIGLLTCLKTLGFFIVGSKKGYQLGELKNLN-LCGSISITHL 658

Query: 1233 EVCTSDV-LENTLRLKLGVRELRLVWNNSVDEYIYIVLDDEGKGVLQCLQLHSKVRTIYI 1292
            E   +D   E  L  K  ++ L + W+N          + +   VL+ L+ H  ++ + I
Sbjct: 659  ERVKNDTDAEANLSAKANLQSLSMSWDNDGPNR----YESKEVKVLEALKPHPNLKYLEI 718

Query: 1293 CKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQFDQFPFLKHLGLKS-LPNIEYIDN 1352
              +       W++ + L  ++S+ +  C     LP F + P L++L L++    +EY++ 
Sbjct: 719  IAFGGFRFPSWINHSVLEKVISVRIKSCKNCLCLPPFGELPCLENLELQNGSAEVEYVEE 778

Query: 1353 NEYP---SSSMFFPSLEELSIVDMHNLKGWWKGETSSESSPNNASFPITMSCLCELEIDA 1412
            ++     S+   FPSL++L I    +LKG  K E           FP+    L E+ I  
Sbjct: 779  DDVHSRFSTRRSFPSLKKLRIWFFRSLKGLMKEE-------GEEKFPM----LEEMAILY 838

Query: 1413 CPHLASIPWHAYLKSLYLREISSQLLNVVIERTTN----NCVEKCST-TSVSLSKIDDLE 1472
            CP             L++    S +  + +   TN    + +   ST TS+ +       
Sbjct: 839  CP-------------LFVFPTLSSVKKLEVHGNTNTRGLSSISNLSTLTSLRIGANYRAT 898

Query: 1473 FLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPINDVLWKEFRSLCYLSLEAIPKLEYL 1532
             LP+ +F   TNLE+L   D KN                                 L+ L
Sbjct: 899  SLPEEMFTSLTNLEFLSFFDFKN---------------------------------LKDL 958

Query: 1533 PNGMKHVTTLERIDIISCPNLITLPE-WIGNFTSLSELMIIVCPKLTSLSERIHHLPSLQ 1592
            P  +  +  L+R+ I SC +L + PE  +   TSL++L +  C  L  L E + HL +L 
Sbjct: 959  PTSLTSLNALKRLQIESCDSLESFPEQGLEGLTSLTQLFVKYCKMLKCLPEGLQHLTALT 987

Query: 1593 DLYIWDCPKLKERYERGKGEDWQKISHIPRVTI 1615
            +L +  CP++++R ++  GEDW KI+HIP + I
Sbjct: 1019 NLGVSGCPEVEKRCDKEIGEDWHKIAHIPNLDI 987

HSP 2 Score: 68.9 bits (167), Expect = 5.8e-10
Identity = 42/140 (30.00%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 805 DVLIQIFQEHLQIFQEEHPFRKCQLFCGVQDELDKLTHTISDMKAFLIDAEEKQFKNKEV 864
           +  +Q+  E+L  F  +    K  L  G + E +KL+   S ++A L DA+EKQ K+K +
Sbjct: 3   EAFLQVLLENLTSFIGD----KLVLIFGFEKECEKLSSVFSTIQAVLQDAQEKQLKDKAI 62

Query: 865 KKWISRVKEAFYDAEDLIDEFAYETLRRQVIANNKRGAKEVRQFFSLPHRIAFRFQMSHK 924
           + W+ ++  A Y+ +D++ E   E +R           ++ R  F  P  I FR ++  +
Sbjct: 63  ENWLQKLNSAAYEVDDILGECKNEAIR----------FEQSRLGFYHPGIINFRHKIGRR 122

Query: 925 IKDIREKLKAINADKNQFTF 945
           +K+I EKL AI+ ++ +F F
Sbjct: 123 MKEIMEKLDAISEERRKFHF 128

BLAST of Cp4.1LG08g13970 vs. ExPASy Swiss-Prot
Match: Q9LRR4 (Putative disease resistance RPP13-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=RPPL1 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 4.0e-35
Identity = 142/502 (28.29%), Postives = 209/502 (41.63%), Query Frame = 0

Query: 211  YTNTSKVLPRSIGKLKHLRYLRIYQRKLEFLPNSITK------------------LYNLE 270
            Y   +   P     L+ +++LR       FLP S+T                   L  L 
Sbjct: 528  YLRDNYAEPMEFEALREVKFLR------TFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLR 587

Query: 271  ILILDGCKCLQELPRDAKNWMKLRYLSLARNMNIKFLPGSMGALHNLETLILQDCSELRE 330
            +L L   K  +  P   KN    R+L L+R   ++ LP S+  ++NL+TL+L  CS L+E
Sbjct: 588  VLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCYMYNLQTLLLSYCSSLKE 647

Query: 331  LPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKL 390
            LP DI   +NL++LDL G   L  M +  G L SLQT+  F +    G  +SEL  L+ L
Sbjct: 648  LPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDL 707

Query: 391  KGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNLVWNYSKDEHMNTI----VDNECNGV 450
             G L I  L+    D  + +  N+  K  ++E++ VW        N        NE   V
Sbjct: 708  HGKLKIVELQ-RVVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAE-V 767

Query: 451  LHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVSIKLSDCFKLQALPQFDQFPFLKY 510
               L+PH  ++K+ I +Y G    DWLS  +   +V I+L +C    +LP   Q P LK 
Sbjct: 768  FEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKE 827

Query: 511  L------GLKSLPNIEYFDNNEY-PSSSMFFPSLEKLSIVEMLNLKGWWKGETSLESSPN 570
            L      GL+S+    YF + +        F SLE L    + + + W     +      
Sbjct: 828  LHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVT-----R 887

Query: 571  NASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLLNVIFERTANHCVEECS 630
               FP     L +L I  CP L       L   + LH     LL+  F+   +       
Sbjct: 888  GDLFP----SLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLD--FQPDHHEYSYRNL 947

Query: 631  STFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHPINDVLWKEFRSLR 684
             T       D L   P     HF NL++LE++ C +L       +H       +  R   
Sbjct: 948  QTLSIKSSCDTLVKFP---LNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRIND 1005

HSP 2 Score: 53.1 bits (126), Expect = 3.3e-05
Identity = 26/71 (36.62%), Postives = 44/71 (61.97%), Query Frame = 0

Query: 51  LHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIA 110
           L +L+  +  + AVL+DAEEKQ  N  V+ W++++++  Y AED +D+ A E LR  + A
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 111 NDKRGAKEVRQ 122
            +   +  +RQ
Sbjct: 99  -ESSSSNRLRQ 108

BLAST of Cp4.1LG08g13970 vs. NCBI nr
Match: XP_023539563.1 (uncharacterized protein LOC111800203 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2845 bits (7375), Expect = 0.0
Identity = 1571/2397 (65.54%), Postives = 1579/2397 (65.87%), Query Frame = 0

Query: 17   MAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLND 76
            MAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLND
Sbjct: 1    MAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLND 60

Query: 77   EVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMS 136
            EVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMS
Sbjct: 61   EVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMS 120

Query: 137  HKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEV----------- 196
            HKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEV           
Sbjct: 121  HKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEVVGRDHDKKAII 180

Query: 197  ------------------------------------------------------------ 256
                                                                        
Sbjct: 181  DLLLNTDPNVKENVAVIAIVGMGGLGKTTLAQFVYNNEDIKKHFELKLWVCISDEFDVKV 240

Query: 257  ------------------------------------------------------------ 316
                                                                        
Sbjct: 241  IVEKILESATRKKPEILQMDLLQSDLRKHIDGKKYLLIMDDVWNEDYERSVALKRLLMGG 300

Query: 317  ------------------------------------------------------------ 376
                                                                        
Sbjct: 301  ASGSRILITTRQQKVAETFDTTSSYALGVLDDNSAWLLFKRMAFGGEEPEKEVVEIGKEI 360

Query: 377  ------------------------------------------------------------ 436
                                                                        
Sbjct: 361  VVKLKGLPLAIRTIGRLLYAKKPEHHLWLAFKDKELSQVLEQREEGEFMRSILGLSYNHL 420

Query: 437  ------------------------------------------------------------ 496
                                                                        
Sbjct: 421  PSHLKQCFLYCALFPKDYDIEKDEVIRQWMAQGFVRSTGAMEASDIGEEYFMELLSRSFF 480

Query: 497  ------------------------------------------------------------ 556
                                                                        
Sbjct: 481  QDVTRNEMGIVTECKMHDLIHDLVCSIVEDEYVSMGVNDSTDIDKIRHISIDNNFAEKLE 540

Query: 557  --------------------EDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKL 616
                                EDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKL
Sbjct: 541  SLKLLFKAKNLRTLIIDAVKEDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKL 600

Query: 617  KHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMN 676
            KHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMN
Sbjct: 601  KHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMN 660

Query: 677  IKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELT 736
            IKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELT
Sbjct: 661  IKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELT 720

Query: 737  SLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQEL 796
            SLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQEL
Sbjct: 721  SLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQEL 780

Query: 797  NLVWNYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVS 856
            NLVWNYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVS
Sbjct: 781  NLVWNYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVS 840

Query: 857  IKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIVEMLN 916
            IKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIVEMLN
Sbjct: 841  IKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIVEMLN 900

Query: 917  LKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLL 976
            LKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLL
Sbjct: 901  LKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLL 960

Query: 977  NVIFERTANHCVEECSSTFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALV 1036
            NVIFERTANHCVEECSSTFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALV
Sbjct: 961  NVIFERTANHCVEECSSTFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALV 1020

Query: 1037 QHPINDVLWKEFRSLRILVLKAIPKLEYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNF 1096
            QHPINDVLWKEFRSLRILVLKAIPKLEYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNF
Sbjct: 1021 QHPINDVLWKEFRSLRILVLKAIPKLEYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNF 1080

Query: 1097 TSLSELMIISCPKLTSLERIYHLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPYSSM 1156
            TSLSELMIISCPKLTSLERIYHLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPY S+
Sbjct: 1081 TSLSELMIISCPKLTSLERIYHLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPYVSI 1140

Query: 1157 SGIIRSTKEEDYYYLQNCSSLKIHSYESSWKKENENRQFDV---------------LIQI 1216
                RSTKEEDYYYLQNCSSLKIHSYESSWKKENEN+  +                L++I
Sbjct: 1141 ESP-RSTKEEDYYYLQNCSSLKIHSYESSWKKENENQDEEEEDEAEEDEAEEISLNLVEI 1200

Query: 1217 FQEHLQIFQEEHP----------FRKCQLFCGVQDELDKLTHTISDMKAFLIDAEEKQFK 1276
                  I  +              ++CQLFCGVQDELDKLTHTISDMKAFLIDAEEKQFK
Sbjct: 1201 LAMAESILFDAATSLITKLGSLAIKECQLFCGVQDELDKLTHTISDMKAFLIDAEEKQFK 1260

Query: 1277 NKEVKKWISRVKEAFYDAEDLIDEFAYETLRRQVIANNKRGAKEVRQFFSLPHRIAFRFQ 1336
            NKEVKKWISRVKEAFYDAEDLIDEFAYETLRRQVIANNKRGAKEVRQFFSLPHRIAFRFQ
Sbjct: 1261 NKEVKKWISRVKEAFYDAEDLIDEFAYETLRRQVIANNKRGAKEVRQFFSLPHRIAFRFQ 1320

Query: 1337 MSHKIKDIREKLKAINADKNQFTFSINTQPEELRKSGDELRKSRETSSFICDDEV----- 1396
            MSHKIKDIREKLKAINADKNQFTFSINTQPEELRKSGDELRKSRETSSFICDDEV     
Sbjct: 1321 MSHKIKDIREKLKAINADKNQFTFSINTQPEELRKSGDELRKSRETSSFICDDEVVGRDH 1380

Query: 1397 ------------------------------------------------------------ 1456
                                                                        
Sbjct: 1381 DKKAIIDLLLNTDPNVKENVAVIAIVGMGGLGKTALAQFAYNNEDIQKHFELKLWVCISD 1440

Query: 1457 ------------------------------------------------------------ 1516
                                                                        
Sbjct: 1441 EFDVKIIVEKILESAIRKKPEIFQMDLLQSELRKNIDGKKYLLIMDDVWNKKDDEWVKLK 1500

Query: 1517 ------------------------------------------------------------ 1576
                                                                        
Sbjct: 1501 RLLMGGASGSKILITTRDRQVATTFDPTSYYVLGELDDKNAWLLFKKMAFGGEEPEKERE 1560

Query: 1577 ------------------------------------------------------------ 1615
                                                                        
Sbjct: 1561 NSNLVDIGKEIVVKLKGLPLAIRTIGRLLYAKKQHHLWLAFKDKEISQVLEQREEAEFML 1620

BLAST of Cp4.1LG08g13970 vs. NCBI nr
Match: XP_022940308.1 (putative disease resistance protein RGA4 [Cucurbita moschata])

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 762/1671 (45.60%), Postives = 901/1671 (53.92%), Query Frame = 0

Query: 3    EAEEISLNLVEILAMAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMK 62
            E EEISLNLV I+AMAESILF+VA SL+TKLGS AI E QLF    D+L KL QTI  M+
Sbjct: 40   EEEEISLNLVGIIAMAESILFNVAASLVTKLGSPAITEFQLFWSFHDDLDKLKQTILAME 99

Query: 63   AVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQF 122
            AVL DAE++Q  +  VKNWIS+VKEAFYD +DLIDE+ YETLR QV+A+ KR  KEVR+ 
Sbjct: 100  AVLHDAEKQQSNSHAVKNWISRVKEAFYDVDDLIDEYTYETLRLQVMADGKRWTKEVRKV 159

Query: 123  FSLPHRIAFRFQMSHKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICD 182
            FSLP +I FRFQMSHKIKDIR+ LKAID DKNQF+ SE MINTRDDELRKRRETSSFICD
Sbjct: 160  FSLPRQIEFRFQMSHKIKDIREKLKAIDDDKNQFHLSECMINTRDDELRKRRETSSFICD 219

Query: 183  DEVEDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKLKHLRYLRIYQRKLEFLP 242
            D+V                                    +G+                  
Sbjct: 220  DDV------------------------------------VGR------------------ 279

Query: 243  NSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMNIKFLPGSMGALHNLETL 302
                          DG K      +   +++   Y ++  N+ +  + G MG L   +T 
Sbjct: 280  --------------DGDK------KAIIDYLLNTYPNVKENVAVIAIVG-MGGLG--KTA 339

Query: 303  ILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSLQTMNLFVLKKDIGCD 362
            + Q       + K                           ELT                 
Sbjct: 340  LAQSVYNNEGIRKHF-------------------------ELT----------------- 399

Query: 363  LSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNLVWNYSKDEHMNTIVD 422
                              L VC +DD +        K+ V+++      S  +H + I+ 
Sbjct: 400  ------------------LWVCISDDFDV-------KVIVEKIL----ESATKHKSEILQ 459

Query: 423  NECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVSIKLSDCFKLQALPQFDQ 482
                  +  LQ   + + ID  KY  +    W                            
Sbjct: 460  ------MDLLQSELR-KNIDGKKYLLIMDDVW---------------------------- 519

Query: 483  FPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIVEMLNLKGWWKGETSLESSPNN 542
                               N +Y          EK   ++ L L G   G   L ++ + 
Sbjct: 520  -------------------NEDY----------EKWVTLKRL-LMGGASGSRILITTRHR 579

Query: 543  ASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLLNVIFERTANHCVEECSS 602
                    R+ E   D   + A            L  +  +   ++F++ A    EE   
Sbjct: 580  --------RVAET-FDTTSYYA------------LGELDDKNAWLLFKKMA-FGGEEPKK 639

Query: 603  TFVSFDK--IDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHPINDVLWKEFRSL 662
              V   K  +  L+ LP            L I     L  +     H     LW  F+  
Sbjct: 640  ELVDIGKEIVVKLKGLP------------LAIRTIGRLLYAKKPEHH-----LWSTFKE- 699

Query: 663  RILVLKAIPKLEYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNFTSLSELMIISCPKLT 722
                           N +  V    +                       E   +    L+
Sbjct: 700  ---------------NELSRVLEQRE-----------------------ETQFM----LS 759

Query: 723  SLERIY-HLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPYSSMSGIIRSTKEEDYYY 782
             LE  Y HLPS        C    + Y+  + E   +     +   +G I+     + Y+
Sbjct: 760  ILELSYNHLPSNLKQCFLYCALFPKDYDIQKDEVIRQWMAHGFVQSTGTIKPNDIGEDYF 819

Query: 783  LQNCSSLKIHSYESSWKKENENRQFDVLIQIFQEHLQIFQEEHPFRKCQLFCGVQDELDK 842
            ++  S         S+ ++    +  ++I                     +C + D    
Sbjct: 820  MELLSR--------SFFQDVTRNEMGIII---------------------YCKMHD---- 879

Query: 843  LTHTISDMKAFLIDAEEKQFKNKEVKKWISRVKEAFYDAEDLIDEFAYETLRRQVIANNK 902
                I D+   +++ E                    Y + D+ D+   E  R  +I    
Sbjct: 880  ---LIHDLACSIVEDE--------------------YVSMDVKDKLVTERTRHILI---- 939

Query: 903  RGAKEVRQFFSLPHRIAFRFQMSHKIKDIREKLKAINADKNQFTFSINTQPEELRKSGDE 962
                                     +K+   KL+++     +  F               
Sbjct: 940  -------------------------LKEFGIKLESL-----KLLFK-------------- 999

Query: 963  LRKSRETSSFICDDEVAKNLRTLIIEASDEDCQKVMNLISTQFLRLRTLTVNFYHTDISK 1022
                            AKNL+TLII+A +EDCQKV+NLISTQFL LRTL V F +T+ SK
Sbjct: 1000 ----------------AKNLKTLIIDAVNEDCQKVVNLISTQFLCLRTLKVEFCYTNTSK 1059

Query: 1023 VLPSFIGKLKHLRYLNICLNKLKFLPNSITKLYNLEILILDGCECLQELPRDAKNWMKLR 1082
            VLPS IGKLKHLRYL IC  KL+FLPNSITKLYNLEILILD CECLQELPRDAKNWMKLR
Sbjct: 1060 VLPSSIGKLKHLRYLRICQRKLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLR 1119

Query: 1083 YLSLAENENIKFLPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKF 1142
            YLSL  N NIKFLPDSI +LH+LETLILDGC+CLQELPR+AKNWKKLRYLSLVGNKNIKF
Sbjct: 1120 YLSLDRNVNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKF 1179

Query: 1143 LPDSI------------------------------------------------TALHNLE 1202
            LPDSI                                                 ALHNLE
Sbjct: 1180 LPDSIGALHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLE 1239

Query: 1203 TLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDIG 1262
            TLILQDCS+LRELPKDIKKCVNLKHL+L GC  LTHMPKGLGELTSLQTMNLFVLKKDIG
Sbjct: 1240 TLILQDCSELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIG 1295

Query: 1263 CDLSELNRLNKLKGTLTIKSLEVCTSDVLENTLRLKLGVRELRLVWNNSVDEYIYIVLDD 1322
            CDLSELNRL+KL+G+LTI+ LEVCT DVLE +L LKLGV+EL LVWN S D+ + IVLD+
Sbjct: 1300 CDLSELNRLDKLEGSLTIEGLEVCTFDVLEKSLPLKLGVQELELVWNYSEDQDMNIVLDN 1295

Query: 1323 EGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQFDQF 1382
            E KG  + LQ HS +RTI IC YPEV LCDWLSSNT VHLVS+ LS CFKLQALPQFDQF
Sbjct: 1360 ECKGGFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFDQF 1295

Query: 1383 PFLKHLGLKSLPNIEYIDNNEYPSSSMFFPSLEELSIVDMHNLKGWWKGETSSESSPNNA 1442
            PFLK+L L+SLPNIEYIDNNEYPSSSMFFPSLEELSIV+M NLKGWWKGETS ESSPNNA
Sbjct: 1420 PFLKYLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESSPNNA 1295

Query: 1443 SFPITMSCLCELEIDACPHLASIPWHAYLKSLYLREISSQLLNVVIERTTNNCVEKCSTT 1502
            SFPI MS LC+LEIDACPHLAS PWHA LKSL L  ISSQLLNVV ERT N+CVE+C +T
Sbjct: 1480 SFPIAMSRLCKLEIDACPHLASFPWHALLKSLSLHAISSQLLNVVFERTANHCVEECFST 1295

Query: 1503 SVSLSKIDDLEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPIN--------DVLWK 1562
             VS  KIDDLEFLP+G FYHFTNLE LEIKDCKNLQMSSALVQHPIN        DVLWK
Sbjct: 1540 FVSFDKIDDLEFLPEGFFYHFTNLEDLEIKDCKNLQMSSALVQHPINSLVQHPINDVLWK 1295

Query: 1563 EFRSLCYLSLEAIPKLEYLPNGMKHVTTLERIDIISCPNLITLPEWIGNFTSLSELMIIV 1614
            EFRSL  L LEAIPKLEYLPNGM+HVTTLERI IISCPNLITLPEWIG+FTSL +LMI+ 
Sbjct: 1600 EFRSLRLLYLEAIPKLEYLPNGMQHVTTLERISIISCPNLITLPEWIGDFTSLLDLMIME 1295

BLAST of Cp4.1LG08g13970 vs. NCBI nr
Match: XP_022931674.1 (putative disease resistance protein RGA1 [Cucurbita moschata] >XP_022931675.1 putative disease resistance protein RGA1 [Cucurbita moschata])

HSP 1 Score: 921 bits (2380), Expect = 6.08e-310
Identity = 553/1147 (48.21%), Postives = 623/1147 (54.32%), Query Frame = 0

Query: 17   MAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLND 76
            MAESILF VA SL TKLGS AI E QLF GV DEL KL  TI  M+AVLLDAEE+Q  + 
Sbjct: 1    MAESILFHVAASLATKLGSPAITEFQLFWGVHDELDKLKHTILTMEAVLLDAEEQQSNSH 60

Query: 77   EVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMS 136
             VKNWIS+VK+AFY+ +DLIDEF YETLR QV+A  KRG KEVRQ FSLPH+IAFRF++S
Sbjct: 61   AVKNWISRVKDAFYEVDDLIDEFTYETLRHQVMAKGKRGVKEVRQVFSLPHQIAFRFKIS 120

Query: 137  HKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEV----------- 196
            HKI+D+R+ LKAIDADKNQF FSER+INTRDDELRK RET SFICDDEV           
Sbjct: 121  HKIRDVREKLKAIDADKNQFYFSERVINTRDDELRKSRETISFICDDEVVGRNPDKKAII 180

Query: 197  ------------------------------------------------------------ 256
                                                                        
Sbjct: 181  DLLLNTDLDTKENIGVIAIVGMGGLGKTALAQSVYNNENITKHFDLKLWVCVSEDFDIKV 240

Query: 257  ------------------------------------------------------------ 316
                                                                        
Sbjct: 241  IVEKIIESATRKKPEILQMDSLQSDLRTHINGKKYLLIMDDVWNEDYEKWVNLKRLLMGG 300

Query: 317  ------------------------------------------------------------ 376
                                                                        
Sbjct: 301  TSGSRILITTRHRRVAETFDTTSCYDLGELDDKSAWLLFKKMALGSEEPGKELENSNLVD 360

Query: 377  ------------------------------------------------------------ 436
                                                                        
Sbjct: 361  IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWLTFKDKELSRVLEQREETQFILSILEL 420

Query: 437  ------------------------------------------------------------ 496
                                                                        
Sbjct: 421  SYHHLPSSLKQCFLYCTLFPKDYNIRKDEVIKQWVAQGFVQSVGVIKPYDIGEDYFMELL 480

Query: 497  ------------------------------------------------------------ 556
                                                                        
Sbjct: 481  SRSFFQDVTRNEMGDIIKFKIHDLMNDLACSIVKKEYVSMDVNDKVVTGRTRHISIYHNF 540

Query: 557  -------------------------EDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPR 616
                                     +D QKV+NLI T+FL LRTL+ D    +T +VLP+
Sbjct: 541  GIKLENLKLLYEAKNLRTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPK 600

Query: 617  SIGKLKHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSL 676
            SIGKLKHLRYL I   +L+FLPNSITKLYNLE LILD C+ LQELPRDAKNWMKLRYLSL
Sbjct: 601  SIGKLKHLRYLNICLYELQFLPNSITKLYNLETLILDRCEHLQELPRDAKNWMKLRYLSL 660

Query: 677  ARNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKG 736
              N  IKFLP S+ +L NLETLILQDCS LRELPKDIKKCVNLKHLDL GCNALTH+ KG
Sbjct: 661  TGNTEIKFLPDSIASLLNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKG 720

Query: 737  LGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKL 766
            LGELT+LQTMNLF+L KD+GCDLSELNRL+KL+G LTIKGLEVCTT+DL+ S FN+R KL
Sbjct: 721  LGELTNLQTMNLFILNKDVGCDLSELNRLSKLRGSLTIKGLEVCTTEDLKNSYFNLRLKL 780

BLAST of Cp4.1LG08g13970 vs. NCBI nr
Match: XP_023528691.1 (putative disease resistance protein RGA1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 855 bits (2208), Expect = 1.85e-287
Identity = 449/744 (60.35%), Postives = 537/744 (72.18%), Query Frame = 0

Query: 37  AIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLI 96
           ++K+C L+C +  + + + +                  ++ +K W+++    F  + ++ 
Sbjct: 203 SLKQCFLYCALFPKDYNIPK------------------DEVIKQWVAQ---GFVQSNNIG 262

Query: 97  DEFAYETLRRQVIANDKRGAK-EVRQF--FSLPHRIAFR------FQMSHKIKDIRDNLK 156
           +++  E L +    +  R    ++ QF    L H +A          M  K + I ++ +
Sbjct: 263 EDYFKELLSKSFFQDVTRNEMGDIIQFKMHDLMHDLACSRVEKEYISMGEKNRVITESTR 322

Query: 157 AIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEVEDCQKVVNLISTQFLCLRTLTV 216
            I+    QF  + + + +    L   +   + I D    + Q V+N I + FL LRTL++
Sbjct: 323 NINIC-GQFGINLKSLQS----LLAAKNLRTLIIDAPGLEGQNVLNPIPSMFLRLRTLSL 382

Query: 217 DFCYTNTSKVLPRSIGKLKHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPR 276
           D     T K L +S+GKLKHLRYL+I Q K  FLPNSITKLYNLE LILD C CLQELP+
Sbjct: 383 DLSCCGTVKALLKSVGKLKHLRYLKICQYKHPFLPNSITKLYNLETLILDWCDCLQELPK 442

Query: 277 DAKNWMKLRYLSLARNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLD 336
           DAKNWMKLRYLSL RN  IKFLP S+  LHNLETLILQ CS LRELPKDIKKCVNLKHLD
Sbjct: 443 DAKNWMKLRYLSLTRNWKIKFLPDSIAELHNLETLILQHCSRLRELPKDIKKCVNLKHLD 502

Query: 337 LHGCNALTHMSKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTD 396
           LHGCNALTH+ KG+GELT LQTMNLFVLKKDIGCDLSELNRLN LKG LT+KGLE CTTD
Sbjct: 503 LHGCNALTHLPKGIGELTRLQTMNLFVLKKDIGCDLSELNRLNNLKGSLTVKGLEACTTD 562

Query: 397 DLEKSSFNMRQKLGVQELNLVWNYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYP 456
           DLEKSS N+  KLGVQ+L LVW YS+D+HMN ++DNEC  VL CLQPHS V++I IC Y 
Sbjct: 563 DLEKSSLNLGLKLGVQKLKLVWTYSEDQHMNNVLDNECKEVLKCLQPHSNVRRIHICGYR 622

Query: 457 GVSLCDWLSSNTLVHLVSIKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPS 516
            V LCDWLSSNTL +LVSIKLSDCFKLQALPQF+QFPFLK+LGLKSL NI+Y DNNEY S
Sbjct: 623 EVRLCDWLSSNTLFNLVSIKLSDCFKLQALPQFNQFPFLKHLGLKSLSNIDYIDNNEYHS 682

Query: 517 SSMFFPSLEKLSIVEMLNLKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFP 576
           SSMFFPSLEKLS+V++ NLKGWWK E S E+SPN ASFPI MS LCELEID CPHLAS P
Sbjct: 683 SSMFFPSLEKLSMVDLHNLKGWWKSENSSEASPNIASFPITMSCLCELEIDGCPHLASIP 742

Query: 577 WHALLKSLRLHAISSQLLNVIFERTANHCVEECSST-----FVSFDKIDDLEFLPEGFFY 636
           W A LKSL L AISSQLLNV+ ERT N+CVEECSST     +    KIDDLEFLPE  FY
Sbjct: 743 WQAPLKSLHLSAISSQLLNVVIERTVNYCVEECSSTQTKVSYFHLSKIDDLEFLPEELFY 802

Query: 637 HFTNLERLEIEDCKNLRMSSALVQHPINDVLWKEFRSLRILVLKAIPKLEYLPNGMQHVT 696
           HFTNLE L+IEDC++L MSSA  QHP NDVLWKEFRSL +L +  IPKL+ LPNG+QHVT
Sbjct: 803 HFTNLELLQIEDCQSLNMSSAFAQHPSNDVLWKEFRSLYLLWISGIPKLDCLPNGIQHVT 862

Query: 697 TLEDIKIISCPNLTTLPEWIGNFTSLSELMIISCPKLTSL-ERIYHLPSLQSLYIWVCPK 756
           TL D++I SCPNL TLPEWI + TSLS+L I +CP L+SL E I+HL SLQ L IW  PK
Sbjct: 863 TLGDLRISSCPNLITLPEWIRDLTSLSQLSIYNCPNLSSLPEEIHHLKSLQFLRIWGSPK 920

Query: 757 LKERYEQGEGEDWPKISHIPYSSM 765
           + ER E+ +GEDWPKIS IPY+ +
Sbjct: 923 MVERCEEEKGEDWPKISRIPYTDI 920

BLAST of Cp4.1LG08g13970 vs. NCBI nr
Match: XP_023539490.1 (putative disease resistance protein RGA3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785 bits (2026), Expect = 5.00e-262
Identity = 392/595 (65.88%), Postives = 462/595 (77.65%), Query Frame = 0

Query: 170 LRKRRETSSFICDDEVEDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKLKHLR 229
           L + ++  + I D  + D +KVV LI T+FL LRTL ++     TS+ LP+SIGKLKHLR
Sbjct: 209 LYEAKKLRTLIIDSPIRDFEKVVKLIFTKFLRLRTLNLNLAIEFTSQALPKSIGKLKHLR 268

Query: 230 YLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMNIKFL 289
           YL+I Q  L FLPNS+T+LYNLE LILDGC  LQ+LPRDAKNW  LRYLSLA N  I+FL
Sbjct: 269 YLKICQIGLCFLPNSMTELYNLETLILDGCMFLQKLPRDAKNWRNLRYLSLAENFRIEFL 328

Query: 290 PGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSLQT 349
           P S+    NLETLIL+ C  L+ELPKDIKKCV LKHLDL GC +LTH  KGLGELT LQT
Sbjct: 329 PDSIDEWKNLETLILRKCLLLKELPKDIKKCVKLKHLDLCGCESLTHSPKGLGELTRLQT 388

Query: 350 MNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNLVW 409
           MNLFVL KD+GCDLSELNRL+KL+G LTI GLE CTTDDLEK SFN++QKLGV++L LVW
Sbjct: 389 MNLFVLNKDVGCDLSELNRLSKLRGSLTINGLEFCTTDDLEKHSFNLQQKLGVRKLKLVW 448

Query: 410 NYSKDEHMNTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVSIKLS 469
           NYSKDE  N   +++C GVL CLQP+S  Q I IC Y  V LCDWL SN L HLVSIKLS
Sbjct: 449 NYSKDEPKNISANDDCKGVLECLQPNSNAQNIYICGYQEVKLCDWLCSNVLTHLVSIKLS 508

Query: 470 DCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIVEMLNLKGW 529
           DCFKL+ALPQFDQFPFLK+LGL+SLP IEY DNNEYPSSSMF PSLEKL +V+M NLKGW
Sbjct: 509 DCFKLEALPQFDQFPFLKHLGLQSLPCIEYVDNNEYPSSSMFLPSLEKLIMVDMPNLKGW 568

Query: 530 WKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLLNVIF 589
           WKGETS ESSPN+ASFP     LC+L+ID+CP LAS  WHA L+ L++  ++ QLLN + 
Sbjct: 569 WKGETSSESSPNSASFPTTAPFLCQLKIDSCPKLASISWHAPLRKLQISDVTLQLLNTVI 628

Query: 590 ERTANHCVEECSS-TFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHP 649
           E TANH     S  + +  +++ DLEFLP   FY+FTNLE L I DCK+L++SS+LVQH 
Sbjct: 629 EMTANHSSSTQSKVSSLELNRMKDLEFLPRELFYNFTNLELLTITDCKSLQISSSLVQHH 688

Query: 650 INDVLWKEFRSLRILVLKAIPKLEYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNFTSL 709
           +N+ LWKEF+SLR L L  IPKLEYLP+GMQHVTTL+ I I  CPNL TLPEW+   TSL
Sbjct: 689 VNEALWKEFQSLRFLHLYEIPKLEYLPDGMQHVTTLQTIYIGCCPNLVTLPEWMDRLTSL 748

Query: 710 SELMIISCPKLTSL-ERIYHLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPY 762
           S+LMI  CP LT+L E I+HLPSL+SL IW CPKL +RYE+G GEDW KISH+P+
Sbjct: 749 SDLMIFQCPMLTTLSEGIHHLPSLKSLQIWDCPKLMKRYEEGRGEDWQKISHVPF 803

BLAST of Cp4.1LG08g13970 vs. ExPASy TrEMBL
Match: A0A6J1FNX2 (putative disease resistance protein RGA4 OS=Cucurbita moschata OX=3662 GN=LOC111445964 PE=4 SV=1)

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 762/1671 (45.60%), Postives = 901/1671 (53.92%), Query Frame = 0

Query: 3    EAEEISLNLVEILAMAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMK 62
            E EEISLNLV I+AMAESILF+VA SL+TKLGS AI E QLF    D+L KL QTI  M+
Sbjct: 40   EEEEISLNLVGIIAMAESILFNVAASLVTKLGSPAITEFQLFWSFHDDLDKLKQTILAME 99

Query: 63   AVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQF 122
            AVL DAE++Q  +  VKNWIS+VKEAFYD +DLIDE+ YETLR QV+A+ KR  KEVR+ 
Sbjct: 100  AVLHDAEKQQSNSHAVKNWISRVKEAFYDVDDLIDEYTYETLRLQVMADGKRWTKEVRKV 159

Query: 123  FSLPHRIAFRFQMSHKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICD 182
            FSLP +I FRFQMSHKIKDIR+ LKAID DKNQF+ SE MINTRDDELRKRRETSSFICD
Sbjct: 160  FSLPRQIEFRFQMSHKIKDIREKLKAIDDDKNQFHLSECMINTRDDELRKRRETSSFICD 219

Query: 183  DEVEDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKLKHLRYLRIYQRKLEFLP 242
            D+V                                    +G+                  
Sbjct: 220  DDV------------------------------------VGR------------------ 279

Query: 243  NSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMNIKFLPGSMGALHNLETL 302
                          DG K      +   +++   Y ++  N+ +  + G MG L   +T 
Sbjct: 280  --------------DGDK------KAIIDYLLNTYPNVKENVAVIAIVG-MGGLG--KTA 339

Query: 303  ILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSLQTMNLFVLKKDIGCD 362
            + Q       + K                           ELT                 
Sbjct: 340  LAQSVYNNEGIRKHF-------------------------ELT----------------- 399

Query: 363  LSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNLVWNYSKDEHMNTIVD 422
                              L VC +DD +        K+ V+++      S  +H + I+ 
Sbjct: 400  ------------------LWVCISDDFDV-------KVIVEKIL----ESATKHKSEILQ 459

Query: 423  NECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVSIKLSDCFKLQALPQFDQ 482
                  +  LQ   + + ID  KY  +    W                            
Sbjct: 460  ------MDLLQSELR-KNIDGKKYLLIMDDVW---------------------------- 519

Query: 483  FPFLKYLGLKSLPNIEYFDNNEYPSSSMFFPSLEKLSIVEMLNLKGWWKGETSLESSPNN 542
                               N +Y          EK   ++ L L G   G   L ++ + 
Sbjct: 520  -------------------NEDY----------EKWVTLKRL-LMGGASGSRILITTRHR 579

Query: 543  ASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLLNVIFERTANHCVEECSS 602
                    R+ E   D   + A            L  +  +   ++F++ A    EE   
Sbjct: 580  --------RVAET-FDTTSYYA------------LGELDDKNAWLLFKKMA-FGGEEPKK 639

Query: 603  TFVSFDK--IDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHPINDVLWKEFRSL 662
              V   K  +  L+ LP            L I     L  +     H     LW  F+  
Sbjct: 640  ELVDIGKEIVVKLKGLP------------LAIRTIGRLLYAKKPEHH-----LWSTFKE- 699

Query: 663  RILVLKAIPKLEYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNFTSLSELMIISCPKLT 722
                           N +  V    +                       E   +    L+
Sbjct: 700  ---------------NELSRVLEQRE-----------------------ETQFM----LS 759

Query: 723  SLERIY-HLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPYSSMSGIIRSTKEEDYYY 782
             LE  Y HLPS        C    + Y+  + E   +     +   +G I+     + Y+
Sbjct: 760  ILELSYNHLPSNLKQCFLYCALFPKDYDIQKDEVIRQWMAHGFVQSTGTIKPNDIGEDYF 819

Query: 783  LQNCSSLKIHSYESSWKKENENRQFDVLIQIFQEHLQIFQEEHPFRKCQLFCGVQDELDK 842
            ++  S         S+ ++    +  ++I                     +C + D    
Sbjct: 820  MELLSR--------SFFQDVTRNEMGIII---------------------YCKMHD---- 879

Query: 843  LTHTISDMKAFLIDAEEKQFKNKEVKKWISRVKEAFYDAEDLIDEFAYETLRRQVIANNK 902
                I D+   +++ E                    Y + D+ D+   E  R  +I    
Sbjct: 880  ---LIHDLACSIVEDE--------------------YVSMDVKDKLVTERTRHILI---- 939

Query: 903  RGAKEVRQFFSLPHRIAFRFQMSHKIKDIREKLKAINADKNQFTFSINTQPEELRKSGDE 962
                                     +K+   KL+++     +  F               
Sbjct: 940  -------------------------LKEFGIKLESL-----KLLFK-------------- 999

Query: 963  LRKSRETSSFICDDEVAKNLRTLIIEASDEDCQKVMNLISTQFLRLRTLTVNFYHTDISK 1022
                            AKNL+TLII+A +EDCQKV+NLISTQFL LRTL V F +T+ SK
Sbjct: 1000 ----------------AKNLKTLIIDAVNEDCQKVVNLISTQFLCLRTLKVEFCYTNTSK 1059

Query: 1023 VLPSFIGKLKHLRYLNICLNKLKFLPNSITKLYNLEILILDGCECLQELPRDAKNWMKLR 1082
            VLPS IGKLKHLRYL IC  KL+FLPNSITKLYNLEILILD CECLQELPRDAKNWMKLR
Sbjct: 1060 VLPSSIGKLKHLRYLRICQRKLEFLPNSITKLYNLEILILDWCECLQELPRDAKNWMKLR 1119

Query: 1083 YLSLAENENIKFLPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKF 1142
            YLSL  N NIKFLPDSI +LH+LETLILDGC+CLQELPR+AKNWKKLRYLSLVGNKNIKF
Sbjct: 1120 YLSLDRNVNIKFLPDSIAALHNLETLILDGCQCLQELPRDAKNWKKLRYLSLVGNKNIKF 1179

Query: 1143 LPDSI------------------------------------------------TALHNLE 1202
            LPDSI                                                 ALHNLE
Sbjct: 1180 LPDSIGALHNLETLILDGCQCLQELPREAKNWMKLRYLSLVRNENIKFLPDFIAALHNLE 1239

Query: 1203 TLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDIG 1262
            TLILQDCS+LRELPKDIKKCVNLKHL+L GC  LTHMPKGLGELTSLQTMNLFVLKKDIG
Sbjct: 1240 TLILQDCSELRELPKDIKKCVNLKHLNLSGCTNLTHMPKGLGELTSLQTMNLFVLKKDIG 1295

Query: 1263 CDLSELNRLNKLKGTLTIKSLEVCTSDVLENTLRLKLGVRELRLVWNNSVDEYIYIVLDD 1322
            CDLSELNRL+KL+G+LTI+ LEVCT DVLE +L LKLGV+EL LVWN S D+ + IVLD+
Sbjct: 1300 CDLSELNRLDKLEGSLTIEGLEVCTFDVLEKSLPLKLGVQELELVWNYSEDQDMNIVLDN 1295

Query: 1323 EGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQFDQF 1382
            E KG  + LQ HS +RTI IC YPEV LCDWLSSNT VHLVS+ LS CFKLQALPQFDQF
Sbjct: 1360 ECKGGFKWLQPHSNLRTIDICGYPEVRLCDWLSSNTFVHLVSIRLSYCFKLQALPQFDQF 1295

Query: 1383 PFLKHLGLKSLPNIEYIDNNEYPSSSMFFPSLEELSIVDMHNLKGWWKGETSSESSPNNA 1442
            PFLK+L L+SLPNIEYIDNNEYPSSSMFFPSLEELSIV+M NLKGWWKGETS ESSPNNA
Sbjct: 1420 PFLKYLTLQSLPNIEYIDNNEYPSSSMFFPSLEELSIVEMLNLKGWWKGETSLESSPNNA 1295

Query: 1443 SFPITMSCLCELEIDACPHLASIPWHAYLKSLYLREISSQLLNVVIERTTNNCVEKCSTT 1502
            SFPI MS LC+LEIDACPHLAS PWHA LKSL L  ISSQLLNVV ERT N+CVE+C +T
Sbjct: 1480 SFPIAMSRLCKLEIDACPHLASFPWHALLKSLSLHAISSQLLNVVFERTANHCVEECFST 1295

Query: 1503 SVSLSKIDDLEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPIN--------DVLWK 1562
             VS  KIDDLEFLP+G FYHFTNLE LEIKDCKNLQMSSALVQHPIN        DVLWK
Sbjct: 1540 FVSFDKIDDLEFLPEGFFYHFTNLEDLEIKDCKNLQMSSALVQHPINSLVQHPINDVLWK 1295

Query: 1563 EFRSLCYLSLEAIPKLEYLPNGMKHVTTLERIDIISCPNLITLPEWIGNFTSLSELMIIV 1614
            EFRSL  L LEAIPKLEYLPNGM+HVTTLERI IISCPNLITLPEWIG+FTSL +LMI+ 
Sbjct: 1600 EFRSLRLLYLEAIPKLEYLPNGMQHVTTLERISIISCPNLITLPEWIGDFTSLLDLMIME 1295

BLAST of Cp4.1LG08g13970 vs. ExPASy TrEMBL
Match: A0A6J1EUW9 (putative disease resistance protein RGA1 OS=Cucurbita moschata OX=3662 GN=LOC111437812 PE=4 SV=1)

HSP 1 Score: 921 bits (2380), Expect = 2.94e-310
Identity = 553/1147 (48.21%), Postives = 623/1147 (54.32%), Query Frame = 0

Query: 17   MAESILFDVATSLITKLGSLAIKECQLFCGVQDELHKLTQTISDMKAVLLDAEEKQFLND 76
            MAESILF VA SL TKLGS AI E QLF GV DEL KL  TI  M+AVLLDAEE+Q  + 
Sbjct: 1    MAESILFHVAASLATKLGSPAITEFQLFWGVHDELDKLKHTILTMEAVLLDAEEQQSNSH 60

Query: 77   EVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIANDKRGAKEVRQFFSLPHRIAFRFQMS 136
             VKNWIS+VK+AFY+ +DLIDEF YETLR QV+A  KRG KEVRQ FSLPH+IAFRF++S
Sbjct: 61   AVKNWISRVKDAFYEVDDLIDEFTYETLRHQVMAKGKRGVKEVRQVFSLPHQIAFRFKIS 120

Query: 137  HKIKDIRDNLKAIDADKNQFNFSERMINTRDDELRKRRETSSFICDDEV----------- 196
            HKI+D+R+ LKAIDADKNQF FSER+INTRDDELRK RET SFICDDEV           
Sbjct: 121  HKIRDVREKLKAIDADKNQFYFSERVINTRDDELRKSRETISFICDDEVVGRNPDKKAII 180

Query: 197  ------------------------------------------------------------ 256
                                                                        
Sbjct: 181  DLLLNTDLDTKENIGVIAIVGMGGLGKTALAQSVYNNENITKHFDLKLWVCVSEDFDIKV 240

Query: 257  ------------------------------------------------------------ 316
                                                                        
Sbjct: 241  IVEKIIESATRKKPEILQMDSLQSDLRTHINGKKYLLIMDDVWNEDYEKWVNLKRLLMGG 300

Query: 317  ------------------------------------------------------------ 376
                                                                        
Sbjct: 301  TSGSRILITTRHRRVAETFDTTSCYDLGELDDKSAWLLFKKMALGSEEPGKELENSNLVD 360

Query: 377  ------------------------------------------------------------ 436
                                                                        
Sbjct: 361  IGKEIVVKLKGLPLAIRTIGRLLYAKKPEHHLWLTFKDKELSRVLEQREETQFILSILEL 420

Query: 437  ------------------------------------------------------------ 496
                                                                        
Sbjct: 421  SYHHLPSSLKQCFLYCTLFPKDYNIRKDEVIKQWVAQGFVQSVGVIKPYDIGEDYFMELL 480

Query: 497  ------------------------------------------------------------ 556
                                                                        
Sbjct: 481  SRSFFQDVTRNEMGDIIKFKIHDLMNDLACSIVKKEYVSMDVNDKVVTGRTRHISIYHNF 540

Query: 557  -------------------------EDCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPR 616
                                     +D QKV+NLI T+FL LRTL+ D    +T +VLP+
Sbjct: 541  GIKLENLKLLYEAKNLRTLIIDAPTKDAQKVLNLIPTKFLRLRTLSFDSSIFSTFQVLPK 600

Query: 617  SIGKLKHLRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSL 676
            SIGKLKHLRYL I   +L+FLPNSITKLYNLE LILD C+ LQELPRDAKNWMKLRYLSL
Sbjct: 601  SIGKLKHLRYLNICLYELQFLPNSITKLYNLETLILDRCEHLQELPRDAKNWMKLRYLSL 660

Query: 677  ARNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKG 736
              N  IKFLP S+ +L NLETLILQDCS LRELPKDIKKCVNLKHLDL GCNALTH+ KG
Sbjct: 661  TGNTEIKFLPDSIASLLNLETLILQDCSLLRELPKDIKKCVNLKHLDLRGCNALTHLPKG 720

Query: 737  LGELTSLQTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKL 766
            LGELT+LQTMNLF+L KD+GCDLSELNRL+KL+G LTIKGLEVCTT+DL+ S FN+R KL
Sbjct: 721  LGELTNLQTMNLFILNKDVGCDLSELNRLSKLRGSLTIKGLEVCTTEDLKNSYFNLRLKL 780

BLAST of Cp4.1LG08g13970 vs. ExPASy TrEMBL
Match: A0A0A0K9P7 (Rx_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G425940 PE=4 SV=1)

HSP 1 Score: 512 bits (1319), Expect = 6.41e-156
Identity = 360/876 (41.10%), Postives = 484/876 (55.25%), Query Frame = 0

Query: 832  GVQDELDKLTHTISDMKAFLIDAEEKQFKNKEVKKWISRVKEAFYDAEDLIDEFAYETLR 891
            GV DELDKL +T+S +KA L+DAEE+Q K+  VK WI+++K+ FYD +DLIDEF+YETLR
Sbjct: 30   GVNDELDKLQNTLSAIKAVLLDAEEQQSKSHTVKDWIAKIKDVFYDIDDLIDEFSYETLR 89

Query: 892  RQVIANNKRGAKEVRQFFSLPHRIAFRFQMSHKIKDIREKLKAINADKNQFTFSINTQPE 951
            RQV+  ++   K+VR FFS  ++IAF F+M   IK +REKL AI A K Q   S+  +  
Sbjct: 90   RQVLTKDRTITKQVRIFFSKSNQIAFGFKMGQTIKKVREKLDAIAAIKAQLHLSVCAR-- 149

Query: 952  ELRKSGDELRKSRETSSFICDDEVAKNLRTLIIEASDEDCQKVMNL-----ISTQFLRLR 1011
            E+R +  E RK RETSSFI + E+ +   + ++    E   K+        +  + L  +
Sbjct: 150  EVRDN--EPRKVRETSSFIPEGEIIELENSNLVRIGKEIVAKLKGYPLSIRVVGRLLYFK 209

Query: 1012 TLTVN---FYHTDISKVL-------PSFIGKLKHL--------RYLNICLNKLKFLPNSI 1071
               ++   F   ++  +L       P       HL         Y  +     +F  N +
Sbjct: 210  NTEMDWSSFKDNELDSILQEDDQIQPILKISFNHLPPKLKQCFTYCALFPKDYEFKKNGL 269

Query: 1072 TK---------LYNLEILILDGCECLQELPRDA------KN-WMKLRYL----------- 1131
             K          +N + +   G +  QEL   +      KN W  L+Y            
Sbjct: 270  VKQWMAQGFIQAHNKKAIEDVGDDYFQELVGRSFFQDIRKNKWGDLKYCKMHDLLHDLAC 329

Query: 1132 SLAENENIKFLPD-----------------------------SITSLHDLE--------- 1191
            S+ ENE +    D                              +TSL  L+         
Sbjct: 330  SIGENECVVVSDDVGSIDKRTRHASFLLSKRLTREVVSKSSIEVTSLRTLDIDSRASFRS 389

Query: 1192 -------------TLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNL 1251
                         TL LD C C    P+     K LRYL+L G  N+ FLP+SIT L+NL
Sbjct: 390  FKKTCHMNLFQLRTLNLDRCCC--HPPKFVDKLKHLRYLNLSG-LNVTFLPNSITTLYNL 449

Query: 1252 ETLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDI 1311
            ETLIL+ C  LR+LPKDI   +NL+HLD++ C++LTHMPKGLG +TSLQTM++FVL K+ 
Sbjct: 450  ETLILRYCLWLRKLPKDINNLINLRHLDIYDCSSLTHMPKGLGGMTSLQTMSMFVLGKNK 509

Query: 1312 GCDLSELNRLNKLKGTLTIKSLEVCTSDVLENTLRLK--LGVRELRLVWNNSVD-EYIYI 1371
            G DLS LN L  L+G L IK L+ CT+  L+N   LK   G+++L L W+  +D E    
Sbjct: 510  GGDLSALNGLKSLRGLLCIKGLQFCTTADLKNVSYLKEMYGIQKLELHWDIKMDHEDALD 569

Query: 1372 VLDDEGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSSNTLVHLVSLELSDCFKLQALPQ 1431
              D++ +GVL+ L+ HS +R + I  Y  + LCDW SSN L  LVS+ELS C KL+ LPQ
Sbjct: 570  DGDNDDEGVLEGLKPHSNIRKMIIKGYRGMKLCDWFSSNFLGGLVSIELSHCEKLEHLPQ 629

Query: 1432 FDQFPFLKHLGLKSLPNIEYIDN-NEYPSSSMFFPSLEELSIVDMHNLKGWWKGETSSES 1491
            FDQF +LKHL L  LPNIEYID+ N   SS+ FFPSLE+L I  M  LKGWWKGE S   
Sbjct: 630  FDQFLYLKHLLLGYLPNIEYIDSGNSVSSSTTFFPSLEKLRIESMPKLKGWWKGEIS--- 689

Query: 1492 SPNNASFPIT-MSCLCELEIDACPHLASIPWHAYLKSLYLREISSQLLNVVIERTTNNCV 1551
                  FP T +  L EL I  CP LASIP H  L+SL +  +S QL  +VI   T+  +
Sbjct: 690  ------FPTTILHQLSELCIFYCPLLASIPQHPSLESLRICGVSVQLFQMVIRMATD--L 749

Query: 1552 EKCSTTSVSLSKID-------DLEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPI- 1591
             + S++S +LSK+        DLEFLP  LF + T+LE L I+ CK+LQMSS    HP+ 
Sbjct: 750  SEHSSSSSTLSKLSFLEIGTIDLEFLPVELFCNMTHLESLIIERCKSLQMSSP---HPVD 809

BLAST of Cp4.1LG08g13970 vs. ExPASy TrEMBL
Match: A0A7J6EF87 (Histone H3.2 OS=Cannabis sativa OX=3483 GN=G4B88_001225 PE=3 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 4.99e-155
Identity = 490/1608 (30.47%), Postives = 737/1608 (45.83%), Query Frame = 0

Query: 187  DCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKLKHLRYLRI-YQRKLEFLPNSI 246
            D +K++NL        R L + FC    S  LP  +G+L++LRYL + +   L  LP+S 
Sbjct: 138  DFEKLINL--------RHLELSFCDKLNS--LPSGVGQLENLRYLDLSWSCGLLSLPDST 197

Query: 247  TKLYNLEILILDGCKCLQELPRDAKNWM------------------------KLRYLSLA 306
              L NL  L L+ C  LQELPRD +  +                        +LRYL L+
Sbjct: 198  NDLLNLRTLKLNSCSNLQELPRDFEKLINLRHLELSSCDKLNSLPSGLGQLTQLRYLDLS 257

Query: 307  RNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGL 366
             N  +  LP S   L NL TL L+ CS+L+ELP+D+ K +NL+HLD+ GC+ L +M  GL
Sbjct: 258  LNDGLLSLPDSTSDLLNLHTLKLRHCSKLQELPRDLWKLINLRHLDISGCDKLEYMPCGL 317

Query: 367  GELTSLQTMNLFVL---KKDI---GCDLSELNRLNKLKGLLTIKGLEVCTTDDL-EKSSF 426
            G+LT+LQ ++ +VL   KK I   G +L EL RLN L+G L +  L  C  D + E  + 
Sbjct: 318  GQLTNLQKLSNYVLMKSKKSIPRHGGELKELMRLNNLRGGLKVTNLS-CEKDVVAEYGTA 377

Query: 427  NMRQKLGVQELNLVWNYSKDEHMN-TIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCD 486
             +  K  ++ L L W+  +DE  +  IV  E +  L  LQPH  +Q++ I KY GV L  
Sbjct: 378  KLTDKQYLRSLVLEWDSDEDEDEDEAIVGYEMS--LEGLQPHQNIQELTIRKYGGVKLSS 437

Query: 487  WLSSNTLVHLVSIKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMF-- 546
            WLSS  L  LV ++L+DC K Q L    Q   LK L L+ L  +EY  N    S  +   
Sbjct: 438  WLSS--LTSLVKLRLADCAKCQHLVPLKQLHCLKNLMLERLVCLEYISNTNNMSEDLIGS 497

Query: 547  ----FPSLEKLSIVEMLNLKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFP 606
                 PSL+ L + ++ NLKGWW+   +      + + P  +  L  L I  CP L   P
Sbjct: 498  TTTLLPSLDSLWLFDLPNLKGWWREIVTCGEEEKHMALPY-LPCLSALRIIDCPKLTRMP 557

Query: 607  WHALLKSLRLHAISSQ-----LLNVIFERTANHCVEECSSTFVSFDKI--------DDLE 666
             +  L    + + +SQ      L  +        +   +S+F    K+        DDLE
Sbjct: 558  LYPHLNGTLMLSKTSQKPFEETLRAMSSNVNVRSIFPTTSSFSPLSKLEWLILENMDDLE 617

Query: 667  FLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHPIN---------------------DVL 726
             LP+ F    T+L  L I  C  L+     + H  +                     D +
Sbjct: 618  CLPDCF-KSLTSLNNLNIRSCPKLKNLCPGILHLSSLRHLGIKNCEGLGDMLNSDDYDFM 677

Query: 727  WKEFRS-LRILVLKAIPKL-EYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNFTSLSEL 786
            W+     L +L L  +P +   LP G+QH+T+L+ I++ SC +LTT+PE I N  SL  L
Sbjct: 678  WQALNGRLNLLNLSKLPNIVSVLPKGIQHLTSLQQIEVHSCDSLTTIPECINNLKSLKTL 737

Query: 787  MIISCPKLTSL-ERIYHLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPYSSMSGIIR 846
             + SCP LTS  E I  L +L  L I  CP L  R ++  GEDW KISHI +  +     
Sbjct: 738  TMDSCPHLTSFPEGIRSLTTLNELKIKDCPMLLRRCKRNTGEDWDKISHIQHLDLLPDPN 797

Query: 847  STKEEDYYYLQNCSSLKIHSYESSWKKENENRQFDVLIQIFQEHLQIFQEEHPFRKCQLF 906
            +T+E +     + +   + +   +             I+ +Q+  ++   + PF++    
Sbjct: 798  NTEENE----NSSNGCNLFNKPGTVALRE--------IRKYQKSTELLIRKLPFQRLVRE 857

Query: 907  CGVQDELDKLTHTISDMKAFLIDAEEKQFKNKEVKKWISR----VKEAFYDAEDL----I 966
               QD    L    S + A    AE       ++  W+S     VK        L    +
Sbjct: 858  IA-QDFKTDLRFQSSAVSALQEAAEAYLIGGVKLSNWLSSHTNLVKLTLKQLHCLKHLEL 917

Query: 967  DEFAYETLRRQVIANNKRGAKEVRQFFSLPHRIAFRFQMSHKI---KDIREKLKAIN--- 1026
            +    E      +AN         +  SLP    F F + +++    D+   L       
Sbjct: 918  ERDGKEVDHSSSVANTNED-----KLISLPSS-PFIFFIENRVLSKADLHATLPIFGNKF 977

Query: 1027 ------ADKNQFTFSINT----QPEEL------------RKSGDELRKSRETSSFICDDE 1086
                    ++ FT S+ +    +P  +            ++  D   + RE  S      
Sbjct: 978  SYREELLQESYFTQSLGSSKLYEPNIMTVLPNGFGIDGEKEKEDRGSRGRERGSRASGSR 1037

Query: 1087 VAKNLRTLIIEASDEDCQKVMNLISTQFL---RLRTLTVNFYHTDISKVLPSFIGKLKHL 1146
              K      ++   E    +   IS        +RTL   F+  D      + I   K L
Sbjct: 1038 ARKREPIFWVDGEKEGADLLGREISASLAYAENVRTLLKFFFSKD-QAFCDAIISNCKFL 1097

Query: 1147 RYLNICLNKLKFLPNSITKLYNLEILILDGCECLQELPRDAKNWMKLRYLSLAENENIKF 1206
            R L++  + +K + NSI KL +                        LRYL L+ N  +K 
Sbjct: 1098 RCLDLKRSSMKLVSNSIGKLIH------------------------LRYLDLSRNSYLKS 1157

Query: 1207 LPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNLE 1266
            LP+SIT+L +L+T  L+ C  L++LP + +    LR+L +    ++  LP  +       
Sbjct: 1158 LPNSITNLVNLQTFKLNYCSNLRKLPGDIEKLINLRHLEVSYCHDLNSLPSGL------- 1217

Query: 1267 TLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDI- 1326
                       ELP+ I+K +NLKHL+++ C+ L +MP GLG+LT+LQ ++ +VL K   
Sbjct: 1218 -----------ELPRYIEKLINLKHLEIYDCDRLEYMPCGLGQLTNLQRLSKYVLMKSEN 1277

Query: 1327 -----GCDLSELNRLNKLKGTLTIKSLEVCTSDVLENTLRLKLG----VRELRLVWNNSV 1386
                 G +L EL RLN L+G L + +L  C  DV     R KL     +R L LVW+++V
Sbjct: 1278 SIPRHGGELKELMRLNNLRGRLEVINLS-CKKDVAAKYERAKLKDKQYLRSLILVWDSNV 1337

Query: 1387 --DEYIYIVLDDEGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSSNTLVHLVSLELSDC 1446
              DE   IV  +     ++ LQ H  +  + +  Y  V L +WLSS+T  +L  L L DC
Sbjct: 1338 EIDETEAIVGYEMS---IEGLQPHQNILELMLVNYGGVKLSNWLSSHT--NLEKLTLKDC 1397

Query: 1447 FKLQALPQFDQFPFLKHLGLKSLPNIEYIDNNEYP-----SSSMFFPSLEELSIVDMHNL 1506
               + L   +Q   LKHL L++L ++EYI N         S++    SL+ L ++ + NL
Sbjct: 1398 ENCRYLVPLEQLQCLKHLKLENLVSLEYISNINMSEGLLGSTTTLLQSLQTLELIRLPNL 1457

Query: 1507 KGWWK-----GETSSESS--PN-----NASFPITMSCLCELEIDACPHLASIPWHAYLKS 1566
            KGWW+     G+    SS  PN     + SFP   S L  L I+ CP L+ +P + +L+ 
Sbjct: 1458 KGWWREIVNSGKEVDHSSFAPNANEVKHTSFPYLPS-LSSLYIECCPKLSCMPLYPHLEG 1517

Query: 1567 -LYLREISSQLLN-----VVIERTTNNCVEKCSTTS-------VSLSKIDDLEFLPKGLF 1614
             L+L   SS+ L      +++     +     S+ S       + L  IDDLE LP   F
Sbjct: 1518 ELHLGNTSSKPLEDTLRMLMLRSNVGSLFPTRSSFSPLSMLERLDLINIDDLECLP-DCF 1577

BLAST of Cp4.1LG08g13970 vs. ExPASy TrEMBL
Match: A0A7J6EWJ3 (Histone H3.2 OS=Cannabis sativa OX=3483 GN=F8388_022405 PE=3 SV=1)

HSP 1 Score: 535 bits (1378), Expect = 1.47e-154
Identity = 490/1608 (30.47%), Postives = 737/1608 (45.83%), Query Frame = 0

Query: 187  DCQKVVNLISTQFLCLRTLTVDFCYTNTSKVLPRSIGKLKHLRYLRI-YQRKLEFLPNSI 246
            D +K++NL        R L + FC    S  LP  +G+L++LRYL + +   L  LP+S 
Sbjct: 138  DFEKLINL--------RHLELSFCDKLNS--LPSGVGQLENLRYLDLSWSCGLLSLPDST 197

Query: 247  TKLYNLEILILDGCKCLQELPRDAKNWM------------------------KLRYLSLA 306
              L NL  L L+ C  LQELPRD +  +                        +LRYL L+
Sbjct: 198  NDLLNLRTLKLNSCSNLQELPRDFEKLINLRHLELSSCDKLNSLPSGLGQLTQLRYLDLS 257

Query: 307  RNMNIKFLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGL 366
             N  +  LP S   L NL TL L+ CS+L+ELP+D+ K +NL+HLD+ GC+ L +M  GL
Sbjct: 258  LNDGLLSLPDSTSDLLNLHTLKLRHCSKLQELPRDLWKLINLRHLDISGCDKLEYMPCGL 317

Query: 367  GELTSLQTMNLFVL---KKDI---GCDLSELNRLNKLKGLLTIKGLEVCTTDDL-EKSSF 426
            G+LT+LQ ++ +VL   KK I   G +L EL RLN L+G L +  L  C  D + E  + 
Sbjct: 318  GQLTNLQKLSNYVLMKSKKSIPRHGGELKELMRLNNLRGGLKVTNLS-CEKDVVAEYGTA 377

Query: 427  NMRQKLGVQELNLVWNYSKDEHMN-TIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCD 486
             +  K  ++ L L W+  +DE  +  IV  E +  L  LQPH  +Q++ I KY GV L  
Sbjct: 378  KLTDKQYLRSLVLEWDSDEDEDEDEAIVGYEMS--LEGLQPHQNIQELTIRKYGGVKLSS 437

Query: 487  WLSSNTLVHLVSIKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEYFDNNEYPSSSMF-- 546
            WLSS  L  LV ++L+DC K Q L    Q   LK L L+ L  +EY  N    S  +   
Sbjct: 438  WLSS--LTSLVKLRLADCAKCQHLVPLKQLHCLKNLMLERLVCLEYISNTNNMSEDLIGS 497

Query: 547  ----FPSLEKLSIVEMLNLKGWWKGETSLESSPNNASFPIAMSRLCELEIDACPHLASFP 606
                 PSL+ L + ++ NLKGWW+   +      + + P  +  L  L I  CP L   P
Sbjct: 498  TTTLLPSLDSLWLFDLPNLKGWWREIVTCGEEEKHMALPY-LPCLSALRIIDCPKLTRMP 557

Query: 607  WHALLKSLRLHAISSQ-----LLNVIFERTANHCVEECSSTFVSFDKI--------DDLE 666
             +  L    + + +SQ      L  +        +   +S+F    K+        DDLE
Sbjct: 558  LYPHLNGTLMLSKTSQKPFEETLRAMSSNVNVRSIFPTTSSFSPLSKLEWLILENMDDLE 617

Query: 667  FLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHPIN---------------------DVL 726
             LP+ F    T+L  L I  C  L+     + H  +                     D +
Sbjct: 618  CLPDCF-KSLTSLNNLNIRSCPKLKNLCPGILHLSSLRHLGIKNCEGLGDMLNSDDYDFM 677

Query: 727  WKEFRS-LRILVLKAIPKL-EYLPNGMQHVTTLEDIKIISCPNLTTLPEWIGNFTSLSEL 786
            W+     L +L L  +P +   LP G+QH+T+L+ I++ SC +LTT+PE I N  SL  L
Sbjct: 678  WQALNGRLNLLNLSKLPNIVSVLPKGIQHLTSLQQIEVHSCDSLTTIPECINNLKSLKTL 737

Query: 787  MIISCPKLTSL-ERIYHLPSLQSLYIWVCPKLKERYEQGEGEDWPKISHIPYSSMSGIIR 846
             + SCP LTS  E I  L +L  L I  CP L  R ++  GEDW KISHI +  +     
Sbjct: 738  TMDSCPHLTSFPEGIRSLTTLNELKIKDCPMLLRRCKRNTGEDWDKISHIQHLDLLPDPN 797

Query: 847  STKEEDYYYLQNCSSLKIHSYESSWKKENENRQFDVLIQIFQEHLQIFQEEHPFRKCQLF 906
            +T+E +     + +   + +   +             I+ +Q+  ++   + PF++    
Sbjct: 798  NTEENE----NSSNGCNLFNKPGTVALRE--------IRKYQKSTELLIRKLPFQRLVRE 857

Query: 907  CGVQDELDKLTHTISDMKAFLIDAEEKQFKNKEVKKWISR----VKEAFYDAEDL----I 966
               QD    L    S + A    AE       ++  W+S     VK        L    +
Sbjct: 858  IA-QDFKTDLRFQSSAVSALQEAAEAYLIGGVKLSNWLSSHTNLVKLTLKQLHCLKHLEL 917

Query: 967  DEFAYETLRRQVIANNKRGAKEVRQFFSLPHRIAFRFQMSHKI---KDIREKLKAIN--- 1026
            +    E      +AN         +  SLP    F F + +++    D+   L       
Sbjct: 918  ERDGKEVDHSSSVANTNED-----KLISLPSS-PFIFFIENRVLSKADLHATLPIFGNKF 977

Query: 1027 ------ADKNQFTFSINT----QPEEL------------RKSGDELRKSRETSSFICDDE 1086
                    ++ FT S+ +    +P  +            ++  D   + RE  S      
Sbjct: 978  SYREELLQESYFTQSLGSSKLYEPNIMTVLPNGFGIDGEKEKEDRGSRGRERGSRASGSR 1037

Query: 1087 VAKNLRTLIIEASDEDCQKVMNLISTQFL---RLRTLTVNFYHTDISKVLPSFIGKLKHL 1146
              K      ++   E    +   IS        +RTL   F+  D      + I   K L
Sbjct: 1038 ARKREPIFWVDGEKEGADLLGREISASLAYAENVRTLLKFFFSKD-QAFCDAIISNCKFL 1097

Query: 1147 RYLNICLNKLKFLPNSITKLYNLEILILDGCECLQELPRDAKNWMKLRYLSLAENENIKF 1206
            R L++  + +K + NSI KL +                        LRYL L+ N  +K 
Sbjct: 1098 RCLDLKRSSMKLVSNSIGKLIH------------------------LRYLDLSRNSYLKS 1157

Query: 1207 LPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKFLPDSITALHNLE 1266
            LP+SIT+L +L+T  L+ C  L++LP + +    LR+L +    ++  LP  +       
Sbjct: 1158 LPNSITNLVNLQTFKLNYCSNLRKLPGDIEKLINLRHLEVSYCHDLNSLPSGL------- 1217

Query: 1267 TLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLKKDI- 1326
                       ELP+ I+K +NLKHL+++ C+ L +MP GLG+LT+LQ ++ +VL K   
Sbjct: 1218 -----------ELPRYIEKLINLKHLEIYDCDRLEYMPCGLGQLTNLQRLSKYVLMKSEN 1277

Query: 1327 -----GCDLSELNRLNKLKGTLTIKSLEVCTSDVLENTLRLKLG----VRELRLVWNNSV 1386
                 G +L EL RLN L+G L + +L  C  DV     R KL     +R L LVW+++V
Sbjct: 1278 SIPRHGGELKELMRLNNLRGRLEVINLS-CKKDVAAKYERAKLKDKQYLRSLILVWDSNV 1337

Query: 1387 --DEYIYIVLDDEGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSSNTLVHLVSLELSDC 1446
              DE   IV  +     ++ LQ H  +  + +  Y  V L +WLSS+T  +L  L L DC
Sbjct: 1338 EIDETEAIVGYEMS---IEGLQPHQNILELMLVNYGGVKLSNWLSSHT--NLEKLTLKDC 1397

Query: 1447 FKLQALPQFDQFPFLKHLGLKSLPNIEYIDNNEYP-----SSSMFFPSLEELSIVDMHNL 1506
               + L   +Q   LKHL L++L ++EYI N         S++    SL+ L ++ + NL
Sbjct: 1398 ENCRYLVPLEQLQCLKHLKLENLVSLEYISNINMSEGLLGSTTTLLQSLQTLELIRLPNL 1457

Query: 1507 KGWWK-----GETSSESS--PN-----NASFPITMSCLCELEIDACPHLASIPWHAYLKS 1566
            KGWW+     G+    SS  PN     + SFP   S L  L I+ CP L+ +P + +L+ 
Sbjct: 1458 KGWWREIVNSGKEVDHSSFAPNANEVKHTSFPYLPS-LSSLYIECCPKLSCMPLYPHLEG 1517

Query: 1567 -LYLREISSQLLN-----VVIERTTNNCVEKCSTTS-------VSLSKIDDLEFLPKGLF 1614
             L+L   SS+ L      +++     +     S+ S       + L  IDDLE LP   F
Sbjct: 1518 ELHLGNTSSKPLEDTLRMLMLRSNVGSLFPTRSSFSPLSMLERLDLINIDDLECLP-DCF 1577

BLAST of Cp4.1LG08g13970 vs. TAIR 10
Match: AT3G14470.1 (NB-ARC domain-containing disease resistance protein )

HSP 1 Score: 152.5 bits (384), Expect = 2.8e-36
Identity = 142/502 (28.29%), Postives = 209/502 (41.63%), Query Frame = 0

Query: 211  YTNTSKVLPRSIGKLKHLRYLRIYQRKLEFLPNSITK------------------LYNLE 270
            Y   +   P     L+ +++LR       FLP S+T                   L  L 
Sbjct: 528  YLRDNYAEPMEFEALREVKFLR------TFLPLSLTNSSRSCCLDQMVSEKLLPTLTRLR 587

Query: 271  ILILDGCKCLQELPRDAKNWMKLRYLSLARNMNIKFLPGSMGALHNLETLILQDCSELRE 330
            +L L   K  +  P   KN    R+L L+R   ++ LP S+  ++NL+TL+L  CS L+E
Sbjct: 588  VLSLSHYKIARLPPDFFKNISHARFLDLSRT-ELEKLPKSLCYMYNLQTLLLSYCSSLKE 647

Query: 331  LPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSLQTMNLFVLKKDIGCDLSELNRLNKL 390
            LP DI   +NL++LDL G   L  M +  G L SLQT+  F +    G  +SEL  L+ L
Sbjct: 648  LPTDISNLINLRYLDLIG-TKLRQMPRRFGRLKSLQTLTTFFVSASDGSRISELGGLHDL 707

Query: 391  KGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNLVWNYSKDEHMNTI----VDNECNGV 450
             G L I  L+    D  + +  N+  K  ++E++ VW        N        NE   V
Sbjct: 708  HGKLKIVELQ-RVVDVADAAEANLNSKKHLREIDFVWRTGSSSSENNTNPHRTQNEAE-V 767

Query: 451  LHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHLVSIKLSDCFKLQALPQFDQFPFLKY 510
               L+PH  ++K+ I +Y G    DWLS  +   +V I+L +C    +LP   Q P LK 
Sbjct: 768  FEKLRPHRHIEKLAIERYKGRRFPDWLSDPSFSRIVCIRLRECQYCTSLPSLGQLPCLKE 827

Query: 511  L------GLKSLPNIEYFDNNEY-PSSSMFFPSLEKLSIVEMLNLKGWWKGETSLESSPN 570
            L      GL+S+    YF + +        F SLE L    + + + W     +      
Sbjct: 828  LHISGMVGLQSIGRKFYFSDQQLRDQDQQPFRSLETLRFDNLPDWQEWLDVRVT-----R 887

Query: 571  NASFPIAMSRLCELEIDACPHLASFPWHALLKSLRLHAISSQLLNVIFERTANHCVEECS 630
               FP     L +L I  CP L       L   + LH     LL+  F+   +       
Sbjct: 888  GDLFP----SLKKLFILRCPELTGTLPTFLPSLISLHIYKCGLLD--FQPDHHEYSYRNL 947

Query: 631  STFVSFDKIDDLEFLPEGFFYHFTNLERLEIEDCKNLRMSSALVQHPINDVLWKEFRSLR 684
             T       D L   P     HF NL++LE++ C +L       +H       +  R   
Sbjct: 948  QTLSIKSSCDTLVKFP---LNHFANLDKLEVDQCTSLYSLELSNEHLRGPNALRNLRIND 1005

HSP 2 Score: 53.1 bits (126), Expect = 2.4e-06
Identity = 26/71 (36.62%), Postives = 44/71 (61.97%), Query Frame = 0

Query: 51  LHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIA 110
           L +L+  +  + AVL+DAEEKQ  N  V+ W++++++  Y AED +D+ A E LR  + A
Sbjct: 39  LERLSTALLTITAVLIDAEEKQITNPVVEKWVNELRDVVYHAEDALDDIATEALRLNIGA 98

Query: 111 NDKRGAKEVRQ 122
            +   +  +RQ
Sbjct: 99  -ESSSSNRLRQ 108

BLAST of Cp4.1LG08g13970 vs. TAIR 10
Match: AT1G69550.1 (disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 146.7 bits (369), Expect = 1.6e-34
Identity = 165/577 (28.60%), Postives = 271/577 (46.97%), Query Frame = 0

Query: 1028 LKHLRYLNICLNKLKFLPNSITKLYNLEILILDGCECLQELPRDAKNWMKLRYLSLAENE 1087
            LK L  + +  ++L+ L   I  L NL+++ L     L+ELP +    + L  + L++  
Sbjct: 669  LKFLVKIILKHSELEKLWEGIQPLVNLKVMDLRYSSHLKELP-NLSTAINLLEMVLSDCS 728

Query: 1088 NIKFLPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKFLPDSITAL 1147
            ++  LP SI +  ++++L + GC  L +LP    N   L  L L+G  ++  LP SI  L
Sbjct: 729  SLIELPSSIGNATNIKSLDIQGCSSLLKLPSSIGNLITLPRLDLMGCSSLVELPSSIGNL 788

Query: 1148 HNLETLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQTMNLFVLK 1207
             NL  L L  CS L ELP  I   +NL+    HGC++L  +P  +G L SL+ + L  + 
Sbjct: 789  INLPRLDLMGCSSLVELPSSIGNLINLEAFYFHGCSSLLELPSSIGNLISLKILYLKRIS 848

Query: 1208 KDIGCDLSELNRLNKLKGTLTIKSLEVCTSDV-LENTLRLKLGVRELRLVWNNSVDEYIY 1267
              +    S  N +N     L + +L  C+S V L +++   + +++L L   +S+ E   
Sbjct: 849  SLVEIPSSIGNLIN-----LKLLNLSGCSSLVELPSSIGNLINLKKLDLSGCSSLVELPL 908

Query: 1268 IVLDDEGKGVLQCLQLHSKVRTIYICKYPEVSLCDWLSS-NTLVHLVSLELSDCFKLQAL 1327
             +      G L  LQ       +Y+ +    SL +  SS   L++L +L LS+C  L  L
Sbjct: 909  SI------GNLINLQ------ELYLSECS--SLVELPSSIGNLINLKTLNLSECSSLVEL 968

Query: 1328 PQFDQFPFLKHLGLKSLPNIEYIDNNEYPSSSMFFPSLEELSIVDMHNLKGWWKGETSSE 1387
            P            + +L N++ +  +E  SS +  PS    SI ++ NLK   K + S  
Sbjct: 969  PS----------SIGNLINLQELYLSEC-SSLVELPS----SIGNLINLK---KLDLSGC 1028

Query: 1388 SSPNNASFPITMSCLCELE---IDACPHLASIPWH----AYLKSLYLREISSQLLNVVIE 1447
            SS      P+++  L  L+   +  C  L  +P        L+ LYL E SS    V + 
Sbjct: 1029 SS--LVELPLSIGNLINLKTLNLSECSSLVELPSSIGNLINLQELYLSECSSL---VELP 1088

Query: 1448 RTTNNCVEKCSTTSVSLSKIDDLEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHP-- 1507
             +  N +   +   + LS    L  LP  +  +  NL+ L +  C      S+LV+ P  
Sbjct: 1089 SSIGNLI---NLKKLDLSGCSSLVELPLSI-GNLINLKTLNLSGC------SSLVELPSS 1148

Query: 1508 INDVLWKEFRSLCYLSLEAIPKLEYLPNGMKHVTTLERIDIISCPNLITLPEWIGNFTSL 1567
            I ++  K+      L L     L  LP+ + ++  L+++D+  C +L+ LP  IGN  +L
Sbjct: 1149 IGNLNLKK------LDLSGCSSLVELPSSIGNLINLKKLDLSGCSSLVELPLSIGNLINL 1186

Query: 1568 SELMIIVCPKLTSLSERIHHLPSLQDLYIWDCPKLKE 1594
             EL +  C  L  L   I +L +LQ+LY+ +C  L E
Sbjct: 1209 QELYLSECSSLVELPSSIGNLINLQELYLSECSSLVE 1186

BLAST of Cp4.1LG08g13970 vs. TAIR 10
Match: AT3G14460.1 (LRR and NB-ARC domains-containing disease resistance protein )

HSP 1 Score: 115.5 bits (288), Expect = 3.9e-25
Identity = 103/354 (29.10%), Postives = 160/354 (45.20%), Query Frame = 0

Query: 228 LRYLRIYQRKLEFLPNSITKLYNLEILILDGCKCLQELPRDAKNWMKLRYLSLARNMNIK 287
           L  L++ ++ L  L N+++    L IL L   + +  LP+  K    LRYL L+ +  IK
Sbjct: 555 LESLQLTEKVLNPLLNALS---GLRILSLSHYQ-ITNLPKSLKGLKLLRYLDLS-STKIK 614

Query: 288 FLPGSMGALHNLETLILQDCSELRELPKDIKKCVNLKHLDLHGCNALTHMSKGLGELTSL 347
            LP  +  L NL+TL+L +C +L  LPK I + +NL+ LDL G   L  M  G+ +L SL
Sbjct: 615 ELPEFVCTLCNLQTLLLSNCRDLTSLPKSIAELINLRLLDLVG-TPLVEMPPGIKKLRSL 674

Query: 348 QTMNLFVLKKDIGCDLSELNRLNKLKGLLTIKGLEVCTTDDLEKSSFNMRQKLGVQELNL 407
           Q ++ FV+ +  G  L EL  L+ L+G L I  L+       E     +++K  +  L L
Sbjct: 675 QKLSNFVIGRLSGAGLHELKELSHLRGTLRISELQNVAFAS-EAKDAGLKRKPFLDGLIL 734

Query: 408 VWNYSKDEHM----NTIVDNECNGVLHCLQPHSKVQKIDICKYPGVSLCDWLSSNTLVHL 467
            W       +    N +  ++   VL  L+PH  ++   I  Y G +   WL  ++   +
Sbjct: 735 KWTVKGSGFVPGSFNALACDQ-KEVLRMLEPHPHLKTFCIESYQGGAFPKWLGDSSFFGI 794

Query: 468 VSIKLSDCFKLQALPQFDQFPFLKYLGLKSLPNIEY----FDNNEYPSSSMFFPSLEKLS 527
            S+ LS C    +LP   Q P LKYL ++    ++     F   E  S  + F SL+ L 
Sbjct: 795 TSVTLSSCNLCISLPPVGQLPSLKYLSIEKFNILQKVGLDFFFGENNSRGVPFQSLQILK 854

Query: 528 IVEMLNLKGWWKGETS-----------LESSPN-NASFPIAMSRLCELEIDACP 562
              M     W   E             ++  P+    FP  +    E+ I  CP
Sbjct: 855 FYGMPRWDEWICPELEDGIFPCLQKLIIQRCPSLRKKFPEGLPSSTEVTISDCP 900

HSP 2 Score: 55.5 bits (132), Expect = 4.7e-07
Identity = 66/222 (29.73%), Postives = 90/222 (40.54%), Query Frame = 0

Query: 1381 ETSSESSPNNASFPITMSCLCELEIDACPHLASIPWH---AYLKSLYLREISSQLLNVVI 1440
            E  +ES PN          L EL I AC  L S P       LK+LY+R+     LN   
Sbjct: 1109 ENLTESYPN----------LHELLIIACHSLESFPGSHPPTTLKTLYIRDCKK--LNFTE 1168

Query: 1441 ERTTNNCVEKCSTTSVSLSKIDDLEFLPKGLFYHFTNLEYLEIKDCKNLQMSSALVQHPI 1500
                     +     +  S   +L   P  L   F  L  L I+DC++ +  S      I
Sbjct: 1169 SLQPTRSYSQLEYLFIG-SSCSNLVNFPLSL---FPKLRSLSIRDCESFKTFS------I 1228

Query: 1501 NDVLWKEFRSLCYLSLEAIPKLEYLPNGMKHVTTLERIDIISCPNLITLPEWIGNFTSLS 1560
            +  L  +  +L  L +   P LE  P G      L  + + +C  L  LPE +   TSL 
Sbjct: 1229 HAGLGDDRIALESLEIRDCPNLETFPQGGLPTPKLSSMLLSNCKKLQALPEKLFGLTSLL 1288

Query: 1561 ELMIIVCPKLTSLSERIHHLPS-LQDLYIWDCPKLKERYERG 1599
             L II CP++ ++       PS L+ L I  C KL  R E G
Sbjct: 1289 SLFIIKCPEIETIPG--GGFPSNLRTLCISLCDKLTPRIEWG 1306

HSP 3 Score: 50.8 bits (120), Expect = 1.2e-05
Identity = 24/60 (40.00%), Postives = 39/60 (65.00%), Query Frame = 0

Query: 51  LHKLTQTISDMKAVLLDAEEKQFLNDEVKNWISKVKEAFYDAEDLIDEFAYETLRRQVIA 110
           L +L   +     VL DA+++     EVK+W++ +K+AF+ AED++DE   E LRR+V+A
Sbjct: 36  LKRLKVALVTANPVLADADQRAEHVREVKHWLTGIKDAFFQAEDILDELQTEALRRRVVA 95

BLAST of Cp4.1LG08g13970 vs. TAIR 10
Match: AT2G14080.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 110.5 bits (275), Expect = 1.2e-23
Identity = 98/324 (30.25%), Postives = 151/324 (46.60%), Query Frame = 0

Query: 1021 LPSFIGKLKHLRYLNIC-LNKLKFLPNSITKLYNLEILILDGCECLQELPRDAKNWMKLR 1080
            LP  IG    L  L +   + L  LP+SI    NL+ +    CE L ELP    N   L+
Sbjct: 713  LPFSIGNATKLLKLELSGCSSLLELPSSIGNAINLQTIDFSHCENLVELPSSIGNATNLK 772

Query: 1081 YLSLAENENIKFLPDSITSLHDLETLILDGCECLQELPREAKNWKKLRYLSLVGNKNIKF 1140
             L L+   ++K LP SI +  +L+ L L  C  L+ELP    N   L+ L L    ++  
Sbjct: 773  ELDLSCCSSLKELPSSIGNCTNLKKLHLICCSSLKELPSSIGNCTNLKELHLTCCSSLIK 832

Query: 1141 LPDSITALHNLETLILQDCSQLRELPKDIKKCVNLKHLDLHGCNALTHMPKGLGELTSLQ 1200
            LP SI    NLE LIL  C  L ELP  I K  NLK L+L   + L  +P  +G L  L 
Sbjct: 833  LPSSIGNAINLEKLILAGCESLVELPSFIGKATNLKILNLGYLSCLVELPSFIGNLHKLS 892

Query: 1201 TMNLFVLKK----DIGCDLSELNRLNKLKGTLTIKSLEVCTSDV------------LENT 1260
             + L   KK        +L  LN L+ L   + +K+  V ++++            + ++
Sbjct: 893  ELRLRGCKKLQVLPTNINLEFLNELD-LTDCILLKTFPVISTNIKRLHLRGTQIEEVPSS 952

Query: 1261 LRLKLGVRELRLVWNNSVDEYIYIVLDDEGKGVLQCLQLHSKVRTIYICKYPEVSLCDWL 1320
            LR    + +L+++++ ++ E+ +++   E   VL+   ++ +  T             WL
Sbjct: 953  LRSWPRLEDLQMLYSENLSEFSHVL---ERITVLELSDINIREMT------------PWL 1012

Query: 1321 SSNTLVHLVSLELSDCFKLQALPQ 1328
              N +  L  L+LS C KL +LPQ
Sbjct: 1013 --NRITRLRRLKLSGCGKLVSLPQ 1018

BLAST of Cp4.1LG08g13970 vs. TAIR 10
Match: AT3G25510.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 104.8 bits (260), Expect = 6.8e-22
Identity = 81/250 (32.40%), Postives = 114/250 (45.60%), Query Frame = 0

Query: 1021 LPSFIGKLKHLRYLNI-CLNKLKFLPNSITKLYNLEILILDG------------------ 1080
            LPS IG   +L+ L++ CL  LK LP SI K  NL+  IL+G                  
Sbjct: 751  LPSSIGNAINLQNLDLGCLRLLK-LPLSIVKFTNLKKFILNGCSSLVELPFMGNATNLQN 810

Query: 1081 -----CECLQELPRDAKNWMKLRYLSLAENENIKFLPDSITSLHDLETLILDGCECLQEL 1140
                 C  L ELP    N + L+ L L+   ++  LP  I +  +LE L L  C  L E+
Sbjct: 811  LDLGNCSSLVELPSSIGNAINLQNLDLSNCSSLVKLPSFIGNATNLEILDLRKCSSLVEI 870

Query: 1141 PREAKNWKKLRYLSLVGNKNIKFLPDSITALHNLETLILQDCSQLRELPKDIKKCVNLKH 1200
            P    +   L  L L G  ++  LP S+  +  L+ L L +CS L +LP       NL  
Sbjct: 871  PTSIGHVTNLWRLDLSGCSSLVELPSSVGNISELQVLNLHNCSNLVKLPSSFGHATNLWR 930

Query: 1201 LDLHGCNALTHMPKGLGELTSLQTM------NLFVLKKDIG-------CDLSELNRLNKL 1234
            LDL GC++L  +P  +G +T+LQ +      NL  L   IG         L+   +L  L
Sbjct: 931  LDLSGCSSLVELPSSIGNITNLQELNLCNCSNLVKLPSSIGNLHLLFTLSLARCQKLEAL 990