Cp4.1LG08g13670 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g13670
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionCytoplasmic tRNA 2-thiolation protein 2
LocationCp4.1LG08: 9804538 .. 9810008 (+)
RNA-Seq ExpressionCp4.1LG08g13670
SyntenyCp4.1LG08g13670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCACAGAAATAGAGCCCAACTCATAGCTTATATCGTCCCCTTCCTAGCTCGTTCTTCTTCGTGCTCTGTCTCTTCTTTTTTTTCTCATTCGTTGGTACTCGTTTTCCTCTTACGACTCATCTCTTTCCCTTAGTCGGTTATTGGTTACACTCTCCTTCGCTTTTGGACTCGTCGCATATATCTCTCTCTTTTAATCTTTCTCATTTTTTCGTTCGTGTTTTGATCAACATCGACCCACTACTGGGATGGTGTGGTTTAGTTACACCAATAACTAGAAACAACCCCGACAACCCTCTAGTAGCAATGAGCGATATCCCCATGAAAGCGTCTGGCTCTATCGTTGCACTGTTGAGATTGTGTCTTTGCGTATAGCAATGATGATGACTTCTTCACACAACTAGTGATCTTCTCCTGGGTATGTAGATTTAAGCAATGATCCAATAAAATAATTCTACGTTGAAGAACGGATATTGGATACTGTATTTTTCTCGAACATCTTGCTATTAGGGTTAGATATGTGTTGCAATTTTTGGTGTAGGTGACTTTACGTGATGTGTTGCAATTTTTGTCGTATGTGATTTTATGTGATGGTTGAGTTAATTAACCATCATATTCGATTGACCCAACGCCCCAAGGAAACGCGCGCCAAGCCAAATCAGACCAAAGTTGGTTTGGTCCAGTCGGGTTGACCTAGAATGGTAAGACCAGACCTTGGCTAGGGTTTGGGTTGTATTAGGCCCTCATTTTGGCGCGTCTGCAAACTTTGCAAGTTCGCAACGAAGCCTTGGCATGCTCTGATTCTCAGGCAACTCACTCTCGACCACCTATGGCGTGTAATTCTTCAACCTGCCAATCCAACTGCTACAGAAATCAAGACGACGAGGCAACAGTCGAACAAGATTCACTTAGAACAAACAAGGCCGATCGTTTAGCCGCCACGAATGGAGACAGTGGTGATACGCATCTCTGCTTTAAATGCAAGGCCAAGGAGCGCATGTCTAGCTCTGAAGAAGGAGACGATGGGCGCTTTTGCAGCGATTGCTTTCGGAGCAACTTGTTTGCAAAGTTCCGGCAGGCTGTAAACTCGAACGCCATGATCTCTCCTTCTGACAAAGTGCTTGTTGCCTTCTCCGGCGGTCCTTGTTCCAGGTCCTTCCTCTTTTTAGACTAATTGTATTTATTTTTTCTCTTGTAATGTATTGGTTCATGGCTGTGGGTACGCAGGGAACTTCGTTTTGGATGTTGCGAGTGGACTTGACAGTTTTCAGACTGATTATTTAGTTAGAATCCCGAGCGCTATTGATATTCTCACGTCTTTCTGTTATTTGTATGCCTCTTTCCGCAGATTGGTTCAAAAGATTTGCAGCTTGTGTATAGTTATTAGGATCCTTGAGCAGGCCTCTAGGTTTATTTGTAGTTAACATGAGTCAATCAATTTTATTTGGTCTAAATTTGAGCATTAATGAAACTTACTTTGTGGCCCAAAATTTATTTGGTCAGGTTGGTGATTAGTCTATTATCTATCTCAGCATGTCCATTGATGGTAACCCCAATAACGATGCTTTCTGAGCGCCAATGAAACCACTTTTCATTAATGAACTCAAAAGCATGTTTAGATACGATAAGAACTAAAGTAACTACCTAGAATTTCTTTGACCATTATAGTGCTTCGCTATCATCGTGCAAACATATTATTTGCTATGCTTTGCCAATACTTCAAGGAAGAACTTAATTGAGTTATATTGACATGAACTTAGCTTAGTGGTTAGGACATTCTACTTCCTCTAAGAGGCTGCATACTTCTTAAGTGCTATCCTTTGCCAATACTTCAAGGAAGGACTTAATTGAGTTATATTGACATGAACTTAGCTTAGTGGTTAGGACATTCTACTTCCTCTAAGAGGCTGCATACTTCTTAAGTGCTATCCTTTGCCAATACTTCAAGGAAGGACTTAATTGAGTTATATTGACATGAACTTAGCTTAGTGGTTAGGACATTCTACTTCCTCTAAGAGGCTGCATACTTCTTAAGTGCTATCCTTTGCCAATACTTCAAGGAAGGACTTAATTGAGTTATATTGACATGAACTTAGCTTAGTGGTTAGGACATTCTACTTCCTCTAAGAGGCTGCATACTTCTTAAGTGCTATCCTTTGCCAATACTTCAAGGAAGAACTTAATAAGTTATATTAACATGCGCTTAGCTCAGCTGTTAAGGCATTCTACTTCCTCTAGGAGGTTGCATTTTTGAATCCTCTCATCCTACATTTGATTCATGATTTATTGAGTTGAATTATTTTCTACCTCTTAGTGATAGAGTTAAAGTGCACAACTTTATATGATCAATGTAGGATATGATTCGTAAGCTGTGTTTTCTTATAAGATAGCTAGAAGAACCGAACTGATTAACATCTTGATAGCTCCTTTTTTTTTTTTTTTTTGATATGAACATCATGATAGCTCTTAGTGATGAATGATCTGGTATTCCTTTAGAAAGTCACCTATACAATTATTTAATCGGATTTTTTTATTACCAATTTATCTTGGATGCACTGGAAACTGTCCATGATCTAAGAAACTTACACTCCCCTTTCCCAACAAAACTTCCAGGGTGGCTTTGCAATTTGTTCATGAGATGCAAGTTAAAGCACACAAAAATTTTGAAGCAAGTAGGGATAGATCACTGCAAGTTTTTGGTGTTGGAGTTGCTTATATCGATGAAACCTCTGCTTGTCCTATCCCTTTGCATGAGATAGACAGAACAATTCAAGCCATCCAATCAGTTGTGTCAAGTCTATCTCCACCGATAAAAGATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAGTGACGGAAGGGAGAAGTTGAAGAAGTTGTTAGATACAGTCAGTGATGTCACAGGAAAGGAAGACCTTATCTCACATTTGCGGATGTTATCCTTACAAAAAGTTTGCTCCTCGACTTATTCCTTCAATTAGCTTGCAATCAAAGAGAGGGAAATTCTTAGTCCTATTGTTTGTTATGCAGATTGCCGCTGAAAATGGATACAACAGACTTGTACTTGGGACGTGTACTTCAAGGATTGCTTGCCATGTGATTTCTTCCACTGTTAAGGTTTTTCTTCAACTGTTAAGCATACATCTACATATTTTAGTTTTAAGCAATTAATTTGAATATTCAAGTACTAGTTTTTTCGGACTCATCATATTGACCGACCAGAATGAAGGTTAGAAGGTTGGTGCATTGCTATTGGATCATTAAGTATGAGCTTGGCAATTGTGGACTTTTAATTATAATTTAGGCATGGCTGCCACTACACTCTGCAGGAAAAATTGATGCTTTGGGGTGGGGGGGTGGTGCATTGTGCCTTTTTGGAGACACCTCTATTAAAATATAGAAAATAGCTAGGGTAGCTATGGAATCTTATTATGTATTATTGTATTGCTTTAAATTTACTGCTAATTATTGTTACAAGCTGCTTATTTTGTTCTGTTCTCCAAATCTTCCCATTTGGGATAGGTCCATGCACTGAGTATGACTTCGCTATTTTAATTCAAGCAGGGCCAAGGATATTCCTTACCGGCAGATATACAGTATGTTGATGCAAGATGGGAGGTCCCAGTGGCACTTCCTCTACGTGATTGTCTTGCACAAGAGCTCAACATGCTTTGCTGTGCTGATGGGTCTGTATTTGTTAGGTTTATCACCAGAATGAATTCTTGGTTAACTTTGTCCTGTTTAGTTCTTTACCGCTGTTAATTTTGCTCGTTGCATAATCTAACTGATGATAAGATCTGGTTCTTGGCAGGGCAGTCTAAAGACAGTGGAACTGACCAAAACATCCTTTTCTGGCATCAATGACTTGGTCTCATCATTCGTGGCTCTATTGCAGGTACAAGTAGGAAGTTGATTTAGAAAAATATGCAGTTTTGTACATTTTAGCTTAGGAGAACGACCTTTGTGAAGGGTAACTAATCTGTTGAATCACTGCTGTGTGCAGCGATAAGACATGTTTGTGTGTTCAATTTACGAGCAATTTTGGTTAGGCATGGAAACAATTTAACTTCTAACGGAACTGCTCTAATTTCACCTTCTAATTCAAGTGATTGTTTCTGAGTAAGAAAGAAAATGCATCTACGAGTTGTGAGGCCACTTGTCCTTCCCAGGACTGTTTTGCAGAACTTGACTGAAGAAATTTTGTTATATTTTCTTTCATAGGAAGAGAATCCCTCTCGTGAGTCGACAATTGTGAGAACTGCTGGCAAGCTTACTCCATTCAACTTTAGCAGAATTCCAGATTTGAATGAAAGTAATGGTGTTCCTTTGGCCACCAAAAGGCGTCAAAAGAGAAATAACTTTAAGCATGTTAACTCTTTATCATCGGAAACATTTTGTGCTGTCTGCAATGGTCCACTTAACATGTCCGATGTGCAAACTTTAAGAAGTTTTGAAGCTGGCCAGGTAAGTTCAATTTTGGCTGGTACCTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCCATATCGCAAGAACTTCTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCAATGCATGGCAGCCCTAGCAATTCAAATTCGCTAAGGTGAGGTTAAGTCTTGTATTACTGTTGGTACTATGAAGGAAAAAGGATAGTGCAAGTTTATGATAACTGAATTTTGTATTCTAAATCTCTGTTGTATATCATCTTCCCACAAGGCTTTTTCCCTTGGATACTGAACCTGGGCATATCAAAAAAATTAAAGTAGAATAAGAAAAATTAAACAACTTCCAGCCTGTGTTTGTATGTTTCTTAAAATGTCTGTATTAAGTCATTTTAGATGGACTTGATGAAATTACTGAAATATTGTTGTTGTTTCATGTAGTTGTATGCTTAAAGTGCGTAGTAGCATGCAGTCTCCTTCAGACATCACTATGTTGAGCTCACTAAGTATTACTTTCAAAATATTCAAGCTGAATATTTCTGTTCTTAATTGCAGGGAGCAAATACAGGAATTCTTGCTGTCAGATGATGAAGTCGAATCGTAAGCCAGGTTAACAACTAGCAAAATTGGCTTCTTTCAACAATGATAATTATACGTGCATTTGTAGAATTTATGGGAAGGCATCGACTTTTCTTTTCTTAGCTGCGGTTTGCTTTCTTTTCGTCTGTTGCATTCAGTTCATTTATATAGAAACAGATTCTTTTACACTCCGGTATATGCTTCTATTTTAGGTTCCTAACATATGCTAGACTGCCTGATTATTCATCACATAAAATGCTGATTATGAAAAATCTTTTAATGTTTAATTCAAGCATCCTGCAAGTGCTTGGATGGAGTAGTAGACGATTAACCTTTCGCGGTAGTATTTGGAGATTTTACCCCCAATTAATGAGAAGTGGCATCCAATCAGTAGAACTGAGACAGGTATTGAGCCTTACCTGTCAT

mRNA sequence

TCACAGAAATAGAGCCCAACTCATAGCTTATATCGTCCCCTTCCTAGCTCGTTCTTCTTCGTGCTCTGTCTCTTCTTTTTTTTCTCATTCGTTGGTACTCGTTTTCCTCTTACGACTCATCTCTTTCCCTTAGTCGGTTATTGGTTACACTCTCCTTCGCTTTTGGACTCGTCGCATATATCTCTCTCTTTTAATCTTTCTCATTTTTTCGTTCGTGTTTTGATCAACATCGACCCACTACTGGGATGGTGTGGTTTAGTTACACCAATAACTAGAAACAACCCCGACAACCCTCTAGTAGCAATGAGCGATATCCCCATGAAAGCGTCTGGCTCTATCGTTGCACTGTTGAGATTGTGTCTTTGCGTATAGCAATGATGATGACTTCTTCACACAACTAGTGATCTTCTCCTGGGTATGTAGATTTAAGCAATGATCCAATAAAATAATTCTACGTTGAAGAACGGATATTGGATACTGTATTTTTCTCGAACATCTTGCTATTAGGGTTAGATATGTGTTGCAATTTTTGGTGTAGGTGACTTTACGTGATGTGTTGCAATTTTTGTCGTATGTGATTTTATGTGATGGTTGAGTTAATTAACCATCATATTCGATTGACCCAACGCCCCAAGGAAACGCGCGCCAAGCCAAATCAGACCAAAGTTGGTTTGGTCCAGTCGGGTTGACCTAGAATGGTAAGACCAGACCTTGGCTAGGGTTTGGGTTGTATTAGGCCCTCATTTTGGCGCGTCTGCAAACTTTGCAAGTTCGCAACGAAGCCTTGGCATGCTCTGATTCTCAGGCAACTCACTCTCGACCACCTATGGCGTGTAATTCTTCAACCTGCCAATCCAACTGCTACAGAAATCAAGACGACGAGGCAACAGTCGAACAAGATTCACTTAGAACAAACAAGGCCGATCGTTTAGCCGCCACGAATGGAGACAGTGGTGATACGCATCTCTGCTTTAAATGCAAGGCCAAGGAGCGCATGTCTAGCTCTGAAGAAGGAGACGATGGGCGCTTTTGCAGCGATTGCTTTCGGAGCAACTTGTTTGCAAAGTTCCGGCAGGCTGTAAACTCGAACGCCATGATCTCTCCTTCTGACAAAGTGCTTGTTGCCTTCTCCGGCGGTCCTTGTTCCAGATTGGTTCAAAAGATTTGCAGCTTGTGTATAGTTATTAGGATCCTTGAGCAGGCCTCTAGGGTGGCTTTGCAATTTGTTCATGAGATGCAAGTTAAAGCACACAAAAATTTTGAAGCAAGTAGGGATAGATCACTGCAAGTTTTTGGTGTTGGAGTTGCTTATATCGATGAAACCTCTGCTTGTCCTATCCCTTTGCATGAGATAGACAGAACAATTCAAGCCATCCAATCAGTTGTGTCAAGTCTATCTCCACCGATAAAAGATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAGTGACGGAAGGGAGAAGTTGAAGAAGTTGTTAGATACAGTCAGTGATGTCACAGGAAAGGAAGACCTTATCTCACATTTGCGGATGTTATCCTTACAAAAAATTGCCGCTGAAAATGGATACAACAGACTTGTACTTGGGACGTGTACTTCAAGGATTGCTTGCCATGTGATTTCTTCCACTGTTAAGGGCCAAGGATATTCCTTACCGGCAGATATACAGTATGTTGATGCAAGATGGGAGGTCCCAGTGGCACTTCCTCTACGTGATTGTCTTGCACAAGAGCTCAACATGCTTTGCTGTGCTGATGGGTCTGTATTTGTTAGATCTGGTTCTTGGCAGGGCAGTCTAAAGACAGTGGAACTGACCAAAACATCCTTTTCTGGCATCAATGACTTGGTCTCATCATTCGTGGCTCTATTGCAGGAAGAGAATCCCTCTCGTGAGTCGACAATTGTGAGAACTGCTGGCAAGCTTACTCCATTCAACTTTAGCAGAATTCCAGATTTGAATGAAAGTAATGGTGTTCCTTTGGCCACCAAAAGGCGTCAAAAGAGAAATAACTTTAAGCATGTTAACTCTTTATCATCGGAAACATTTTGTGCTGTCTGCAATGGTCCACTTAACATGTCCGATGTGCAAACTTTAAGAAGTTTTGAAGCTGGCCAGGTAAGTTCAATTTTGGCTGGTACCTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCCATATCGCAAGAACTTCTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCAATGCATGGCAGCCCTAGCAATTCAAATTCGCTAAGGGAGCAAATACAGGAATTCTTGCTGTCAGATGATGAAGTCGAATCGTAAGCCAGCATCCTGCAAGTGCTTGGATGGAGTAGTAGACGATTAACCTTTCGCGGTAGTATTTGGAGATTTTACCCCCAATTAATGAGAAGTGGCATCCAATCAGTAGAACTGAGACAGGTATTGAGCCTTACCTGTCAT

Coding sequence (CDS)

ATGGCGTGTAATTCTTCAACCTGCCAATCCAACTGCTACAGAAATCAAGACGACGAGGCAACAGTCGAACAAGATTCACTTAGAACAAACAAGGCCGATCGTTTAGCCGCCACGAATGGAGACAGTGGTGATACGCATCTCTGCTTTAAATGCAAGGCCAAGGAGCGCATGTCTAGCTCTGAAGAAGGAGACGATGGGCGCTTTTGCAGCGATTGCTTTCGGAGCAACTTGTTTGCAAAGTTCCGGCAGGCTGTAAACTCGAACGCCATGATCTCTCCTTCTGACAAAGTGCTTGTTGCCTTCTCCGGCGGTCCTTGTTCCAGATTGGTTCAAAAGATTTGCAGCTTGTGTATAGTTATTAGGATCCTTGAGCAGGCCTCTAGGGTGGCTTTGCAATTTGTTCATGAGATGCAAGTTAAAGCACACAAAAATTTTGAAGCAAGTAGGGATAGATCACTGCAAGTTTTTGGTGTTGGAGTTGCTTATATCGATGAAACCTCTGCTTGTCCTATCCCTTTGCATGAGATAGACAGAACAATTCAAGCCATCCAATCAGTTGTGTCAAGTCTATCTCCACCGATAAAAGATTTGCATATTGTTCAAATTGAGAGTGTATACTCTTCAAATTTGAGTGACGGAAGGGAGAAGTTGAAGAAGTTGTTAGATACAGTCAGTGATGTCACAGGAAAGGAAGACCTTATCTCACATTTGCGGATGTTATCCTTACAAAAAATTGCCGCTGAAAATGGATACAACAGACTTGTACTTGGGACGTGTACTTCAAGGATTGCTTGCCATGTGATTTCTTCCACTGTTAAGGGCCAAGGATATTCCTTACCGGCAGATATACAGTATGTTGATGCAAGATGGGAGGTCCCAGTGGCACTTCCTCTACGTGATTGTCTTGCACAAGAGCTCAACATGCTTTGCTGTGCTGATGGGTCTGTATTTGTTAGATCTGGTTCTTGGCAGGGCAGTCTAAAGACAGTGGAACTGACCAAAACATCCTTTTCTGGCATCAATGACTTGGTCTCATCATTCGTGGCTCTATTGCAGGAAGAGAATCCCTCTCGTGAGTCGACAATTGTGAGAACTGCTGGCAAGCTTACTCCATTCAACTTTAGCAGAATTCCAGATTTGAATGAAAGTAATGGTGTTCCTTTGGCCACCAAAAGGCGTCAAAAGAGAAATAACTTTAAGCATGTTAACTCTTTATCATCGGAAACATTTTGTGCTGTCTGCAATGGTCCACTTAACATGTCCGATGTGCAAACTTTAAGAAGTTTTGAAGCTGGCCAGGTAAGTTCAATTTTGGCTGGTACCTGTTGTTCAAGTTGTCAATTTCAGATTCTTCCCAAGGATTCCATATCGCAAGAACTTCTTCTTTCACTCTTGCCACCCTCTATGGTTGCTCGAGCAATGCATGGCAGCCCTAGCAATTCAAATTCGCTAAGGGAGCAAATACAGGAATTCTTGCTGTCAGATGATGAAGTCGAATCGTAA

Protein sequence

MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVIRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSLREQIQEFLLSDDEVES
Homology
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BLAST of Cp4.1LG08g13670 vs. ExPASy Swiss-Prot
Match: O65628 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 PE=2 SV=3)

HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-143
Identity = 275/498 (55.22%), Postives = 343/498 (68.88%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS C+S CY  + D  +   D           A +G      +C KCK    M+  
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDD----------AVSGGGNHESVCVKCKCNAPMTFG 60

Query: 61  EEG-DDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIV 120
           + G DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG               
Sbjct: 61  DGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGG--------------- 120

Query: 121 IRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRT 180
                 +SRV+LQFVHE+Q+KA KN+EASRDRSL VFGVGVA++DET+A P    E+   
Sbjct: 121 -----SSSRVSLQFVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDA 180

Query: 181 IQAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRM 240
           I+ ++  VS LSPP KDLH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ HL+M
Sbjct: 181 IEWVRYTVSCLSPPAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKM 240

Query: 241 LSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLR 300
           LSLQK+AAENGYNRLVLG+CTSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLR
Sbjct: 241 LSLQKVAAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLR 300

Query: 301 DCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRE 360
           DC+  E+  LC  DG            LKTVEL   S  GINDLVSSFVALLQEENPSRE
Sbjct: 301 DCVRLEITRLCLLDG------------LKTVELACRSQCGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420
            TIVRTA KLTPF F++IP+ ++SN VP+AT+RR KR N K+  S+++E FC +CNGPLN
Sbjct: 361 CTIVRTAAKLTPFYFNKIPETDDSN-VPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLN 420

Query: 421 MSDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPS 480
            SD   L +FE GQ S +L   CCSSC+FQILP+D  S E   S LP  M+++  H    
Sbjct: 421 RSDSSELDTFEEGQESDVLYAACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVD 455

Query: 481 NSNSLREQIQEFLLSDDE 498
           +   LRE+I++ LL DDE
Sbjct: 481 SQAYLREKIKDCLLLDDE 455

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy Swiss-Prot
Match: Q2QMW0 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CTU2 PE=2 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 7.1e-100
Identity = 214/498 (42.97%), Postives = 293/498 (58.84%), Query Frame = 0 

Query: 2   ACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSE 61
           +C  + C  +C  +    A  +  +    K  RL+ +         C KC       +  
Sbjct: 8   SCGGAGCGPHCSSSASAAAVEDAAAAAAEKVGRLSLSRE-------CGKCGGGAAAVAVA 67

Query: 62  EGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVIR 121
            G     C +CFR+NL  KF+ AV SNAM+ P+D VL+AFSGGP                
Sbjct: 68  GGLG--LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGP---------------- 127

Query: 122 ILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQ 181
               ASRVALQF+HEM+ KA ++++ S  ++L VFGVGVA++DE+  C  P  EI+  I+
Sbjct: 128 ----ASRVALQFIHEMRCKAIESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIE 187

Query: 182 AIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLS 241
            I+S+VSSLS  +K +HI ++E V+S+   DG  +L++ +D + D TG+ED +  LRMLS
Sbjct: 188 DIRSIVSSLSTGVKAMHIARLEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLS 247

Query: 242 LQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC 301
           LQKIA ENGY +++LG+C S IACHV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDC
Sbjct: 248 LQKIAMENGYAKIMLGSCASAIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDC 307

Query: 302 LAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSREST 361
           LAQEL +LC  D            SLKT +      +GIN LV+SF+  L+EENPSRE T
Sbjct: 308 LAQELTLLCELD------------SLKTQQHLDRPSNGINSLVASFIKRLREENPSREHT 367

Query: 362 IVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMS 421
           IVRTA KL PF+F++       + +P  ++ R K   F    S  SE  C +C  P + S
Sbjct: 368 IVRTAQKLKPFSFNKFSADGYHDFLP--SRLRPKFQKFDSDESTFSEILCLMCGSPFSES 427

Query: 422 DVQTLRS--FEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPS 481
           ++Q L S   +A +   +    CC SC FQILP      E   SLLP     +    S S
Sbjct: 428 ELQNLESTKHKAQKKIDLYTAHCCQSCYFQILPAGENLNEHFFSLLPKLWTGKMDTISDS 461

Query: 482 NSNSLREQIQEFLLSDDE 498
           +S  LR+QI+E+LL +++
Sbjct: 488 HS-LLRDQIEEYLLEEND 461

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy Swiss-Prot
Match: A2ZM73 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 GN=CTU2 PE=3 SV=1)

HSP 1 Score: 364.8 bits (935), Expect = 1.6e-99
Identity = 214/498 (42.97%), Postives = 293/498 (58.84%), Query Frame = 0 

Query: 2   ACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSE 61
           +C  + C  +C  +    A  +  +    K  RL+ +         C KC       +  
Sbjct: 8   SCGGAGCGPHCSSSASAAAVEDAAAAAAEKVGRLSLSRE-------CGKCGGGAAAVAVA 67

Query: 62  EGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVIR 121
            G     C +CFR+NL  KF+ AV SNAM+ P+D VL+AFSGGP                
Sbjct: 68  GGLG--LCGECFRANLLGKFKLAVTSNAMVRPTDSVLLAFSGGP---------------- 127

Query: 122 ILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQ 181
               ASRVALQF+HEM+ KA ++++ S  ++L VFGVGVA++DE+  C  P  EI+  I+
Sbjct: 128 ----ASRVALQFIHEMRCKAIESWDVSNSQALPVFGVGVAFVDESVLCSKPRDEIEMAIE 187

Query: 182 AIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLS 241
            I+S+VSSLS  +K +HIV++E V+S+   DG  +L++ +D + D TG+ED +  LRMLS
Sbjct: 188 DIRSIVSSLSTGVKAMHIVRLEDVFSTESEDGERRLREAVDMIGDDTGREDFLRCLRMLS 247

Query: 242 LQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDC 301
           LQKIA ENGY +++LG+C S IACHV+S+TVKGQGYSLPAD+QYVD RWE+PV LPLRDC
Sbjct: 248 LQKIAMENGYAKIMLGSCASAIACHVLSATVKGQGYSLPADVQYVDTRWEIPVVLPLRDC 307

Query: 302 LAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSREST 361
           LAQEL +LC  D            SLKT +      +GIN LV+SF+  L+EENPSRE T
Sbjct: 308 LAQELTLLCELD------------SLKTQQHLDRPSNGINSLVASFIKRLREENPSREHT 367

Query: 362 IVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMS 421
           IVRTA KL PF+F++       + +P  ++ R K        S  SE  C +C  P + S
Sbjct: 368 IVRTAQKLKPFSFNKFSADGYHDFLP--SRLRPKFQKVDSDESTFSEILCLMCGSPFSES 427

Query: 422 DVQTLRS--FEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPS 481
           ++Q L S   +A +   +    CC SC FQILP      E   SLLP     +    S S
Sbjct: 428 ELQNLESTKHKAQKKIDLYTAHCCQSCYFQILPAGENLNEHFFSLLPKLWTGKMDTISDS 461

Query: 482 NSNSLREQIQEFLLSDDE 498
           +S  LR+QI+E+LL +++
Sbjct: 488 HS-LLRDQIEEYLLEEND 461

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy Swiss-Prot
Match: Q08B12 (Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 8.1e-19
Identity = 133/542 (24.54%), Postives = 211/542 (38.93%), Query Frame = 0 

Query: 47  LCFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPC 106
           +C KCK          GD   FC  CF+     KFR  +  N +I P +KVL+A+SGGP 
Sbjct: 24  ICMKCKESSAALLIRAGD--AFCKSCFKEYFVHKFRATLGKNRVIYPGEKVLLAYSGGPS 83

Query: 107 SRLVQKICSLCIVIRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDET 166
           S          +V ++ E  SR A + +                     F  G+ +IDE 
Sbjct: 84  SS--------AMVRQVQEGLSRDAPKKLR--------------------FVPGILFIDEG 143

Query: 167 SACPIPLHEIDRTIQAIQSVVSSLSPPIKDLHIVQIESVYS---SNLSDG---------- 226
           +AC +   E  + +  I SV+     P    HIV +E V+S   S L  G          
Sbjct: 144 TACGMSWEERQQILSEICSVLQQTKIP---FHIVSLEQVFSLPGSVLQRGAPEQRPNYKE 203

Query: 227 --------------------------------------------------REKLKKLLDT 286
                                                              +KLK+L  +
Sbjct: 204 EVDRFLVQEREQGDAGCSEMLERLEVTDSDSPGSSDKMYQSTCSHPPDMHTQKLKQLFAS 263

Query: 287 VSDVTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADI 346
              +T K+ L+  LR   +  IA   GY++++ G   +R++  ++S+   G+G  LP D 
Sbjct: 264 AKTLTAKQQLLHTLRSHLILHIARTCGYSKVMTGESCTRLSIRLLSNVSLGRGAFLPLDT 323

Query: 347 QYVDARW-EVPVALPLRDCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGIND 406
            + D+R+ +V +  P+R+  ++E+          F  S  +  +L T     +  S I  
Sbjct: 324 GFCDSRYGDVDIIRPMREYSSKEIAYY----NRFFNVSPIFIPALDT---KASENSSIQH 383

Query: 407 LVSSFVALLQEENPSRESTIVRTAGKLTPFNFSRI----------------PDLNESNGV 466
           L   FV  LQ + PS  ST+ RT+ KL   N S+I                  L+   G 
Sbjct: 384 LTEVFVNRLQADFPSTVSTLYRTSEKL---NVSKIDADQETCAKDRCLLCLSPLDTQAGK 443

Query: 467 PLATKRRQKRNNFKH-----VNSLSSETFCAVCNGPLNMSDV---QTLRSFEAGQVSSIL 501
             A    Q  ++         N L++ +  + C G     +     T +S  A Q  S +
Sbjct: 444 ASAFSATQLSHHLSQKIPMKSNDLANNSDKSCCQGGQGCKEAGYGDTCQS-RALQTPSFV 503

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy Swiss-Prot
Match: Q2VPK5 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Homo sapiens OX=9606 GN=CTU2 PE=1 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.4e-17
Identity = 97/387 (25.06%), Postives = 157/387 (40.57%), Query Frame = 0 

Query: 48  CFKCKAKERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCS 107
           C KCK  + +     GD   FC DCF++    KFR  +  N +I P +KVL+A+SGGP S
Sbjct: 28  CVKCKEAQPVVVIRAGD--AFCRDCFKAFYVHKFRAMLGKNRLIFPGEKVLLAWSGGPSS 87

Query: 108 RLVQKICSLCIVIRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETS 167
             +     +  V+  L Q S   L+FV                        GV ++DE +
Sbjct: 88  SSM-----VWQVLEGLSQDSAKRLRFV-----------------------AGVIFVDEGA 147

Query: 168 ACPIPLHEIDRTIQAIQSVVSSLSPPIKDLHIVQIESVYS-------------------- 227
           AC   L E  +T+  ++ ++ +   P    H+V +E V+S                    
Sbjct: 148 ACGQSLEERSKTLAEVKPILQATGFP---WHVVALEEVFSLPPSVLWCSAQELVGSEGAY 207

Query: 228 ------------------------------------SNLS-----DGREKLKKLLDTVSD 287
                                                NL+        E L +L  +V  
Sbjct: 208 KAAVDSFLQQQHVLGAGGGPGPTQGEEQPPQPPLDPQNLARPPAPAQTEALSQLFCSVRT 267

Query: 288 VTGKEDLISHLRMLSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYV 347
           +T KE+L+  LR   +  +A  +GY++++ G   +R+A  ++++   G+G  L  D  + 
Sbjct: 268 LTAKEELLQTLRTHLILHMARAHGYSKVMTGDSCTRLAIKLMTNLALGRGAFLAWDTGFS 327

Query: 348 DAR-WEVPVALPLRDCLAQEL---NMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGIND 370
           D R  +V V  P+RD   +E+   N L     SVF            V+      + I+ 
Sbjct: 328 DERHGDVVVVRPMRDHTLKEVAFYNRLFSVP-SVFT---------PAVDTKAPEKASIHR 371

[Close]
BLAST of Cp4.1LG08g13670 vs. NCBI nr
Match: XP_023539180.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 900 bits (2326), Expect = 0.0
Identity = 468/500 (93.60%), Postives = 468/500 (93.60%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVI 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR            
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR------------ 120

Query: 121 RILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180
                   VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI
Sbjct: 121 --------VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180

Query: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240
           QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML
Sbjct: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240

Query: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300
           SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD
Sbjct: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300

Query: 301 CLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360
           CLAQELNMLCCADG            LKTVELTKTSFSGINDLVSSFVALLQEENPSRES
Sbjct: 301 CLAQELNMLCCADG------------LKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420
           TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420

Query: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 480
           SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN
Sbjct: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 468

Query: 481 SNSLREQIQEFLLSDDEVES 500
           SNSLREQIQEFLLSDDEVES
Sbjct: 481 SNSLREQIQEFLLSDDEVES 468

[Close]
BLAST of Cp4.1LG08g13670 vs. NCBI nr
Match: KAG7027733.1 (Cytoplasmic tRNA 2-thiolation protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 897 bits (2318), Expect = 0.0
Identity = 466/496 (93.95%), Postives = 467/496 (94.15%), Query Frame = 0 

Query: 5   SSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSEEGD 64
           SS CQSNC+RNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSEEGD
Sbjct: 17  SSACQSNCHRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSSEEGD 76

Query: 65  DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVIRILE 124
           DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR                
Sbjct: 77  DGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR---------------- 136

Query: 125 QASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQAIQ 184
               VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQ IQ
Sbjct: 137 ----VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTIQTIQ 196

Query: 185 SVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK 244
           SVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK
Sbjct: 197 SVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRMLSLQK 256

Query: 245 IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQ 304
           IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQ
Sbjct: 257 IAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRDCLAQ 316

Query: 305 ELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVR 364
           ELNMLCCADGSVF       GSLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVR
Sbjct: 317 ELNMLCCADGSVF-------GSLKTVELTKTSFSGINDLVSSFVALLQEENPSRESTIVR 376

Query: 365 TAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQ 424
           TAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQ
Sbjct: 377 TAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNMSDVQ 436

Query: 425 TLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSL 484
           TLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSL
Sbjct: 437 TLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSNSNSL 485

Query: 485 REQIQEFLLSDDEVES 500
           REQIQEFLLSDDEVES
Sbjct: 497 REQIQEFLLSDDEVES 485

[Close]
BLAST of Cp4.1LG08g13670 vs. NCBI nr
Match: XP_022939604.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata])

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 465/500 (93.00%), Postives = 465/500 (93.00%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS CQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVI 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR            
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR------------ 120

Query: 121 RILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180
                   VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI
Sbjct: 121 --------VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180

Query: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240
           Q IQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML
Sbjct: 181 QTIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240

Query: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300
           SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD
Sbjct: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300

Query: 301 CLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360
           CLAQELNMLCCADG            LKT ELTKTSFSGINDLVSSFVALLQEENPSRES
Sbjct: 301 CLAQELNMLCCADG------------LKTEELTKTSFSGINDLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420
           TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420

Query: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 480
           SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN
Sbjct: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 468

Query: 481 SNSLREQIQEFLLSDDEVES 500
           SNSLREQIQEFLLSDDEVES
Sbjct: 481 SNSLREQIQEFLLSDDEVES 468

[Close]
BLAST of Cp4.1LG08g13670 vs. NCBI nr
Match: XP_022971223.1 (cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima])

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 462/500 (92.40%), Postives = 463/500 (92.60%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS CQSNCYRNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVI 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR            
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR------------ 120

Query: 121 RILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180
                   VALQFVHEMQVKAHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTI
Sbjct: 121 --------VALQFVHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTI 180

Query: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240
           Q IQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML
Sbjct: 181 QTIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240

Query: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300
           SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD
Sbjct: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300

Query: 301 CLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360
           CLAQELNMLCCADG            LKTVELTKTSFSGINDLVSSFVALLQEENPSRES
Sbjct: 301 CLAQELNMLCCADG------------LKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420
           TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420

Query: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 480
           SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN
Sbjct: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 468

Query: 481 SNSLREQIQEFLLSDDEVES 500
           SNSLRE+IQEFLLSDDEVES
Sbjct: 481 SNSLREKIQEFLLSDDEVES 468

[Close]
BLAST of Cp4.1LG08g13670 vs. NCBI nr
Match: KAG6596194.1 (Cytoplasmic tRNA 2-thiolation protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 816 bits (2109), Expect = 2.60e-296
Identity = 433/500 (86.60%), Postives = 435/500 (87.00%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS CQSNC+RNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCHRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVI 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR            
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR------------ 120

Query: 121 RILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180
                   VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI
Sbjct: 121 --------VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180

Query: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240
           Q IQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML
Sbjct: 181 QTIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240

Query: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300
           SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD
Sbjct: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300

Query: 301 CLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360
           CLAQELNMLCCADG            LKTVELTKTSFS INDLVSSFVALLQEENPSRES
Sbjct: 301 CLAQELNMLCCADG------------LKTVELTKTSFSSINDLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420
           TIVRTAGKLTPFNFSRIPDLNE                              +CNGPLNM
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNE------------------------------ICNGPLNM 420

Query: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 480
           SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN
Sbjct: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 438

Query: 481 SNSLREQIQEFLLSDDEVES 500
           SNSLREQIQEFLLSDDEVES
Sbjct: 481 SNSLREQIQEFLLSDDEVES 438

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy TrEMBL
Match: A0A6J1FHP7 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111445447 PE=3 SV=1)

HSP 1 Score: 894 bits (2311), Expect = 0.0
Identity = 465/500 (93.00%), Postives = 465/500 (93.00%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS CQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVI 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR            
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR------------ 120

Query: 121 RILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180
                   VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI
Sbjct: 121 --------VALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180

Query: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240
           Q IQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML
Sbjct: 181 QTIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240

Query: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300
           SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD
Sbjct: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300

Query: 301 CLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360
           CLAQELNMLCCADG            LKT ELTKTSFSGINDLVSSFVALLQEENPSRES
Sbjct: 301 CLAQELNMLCCADG------------LKTEELTKTSFSGINDLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420
           TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420

Query: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 480
           SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN
Sbjct: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 468

Query: 481 SNSLREQIQEFLLSDDEVES 500
           SNSLREQIQEFLLSDDEVES
Sbjct: 481 SNSLREQIQEFLLSDDEVES 468

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy TrEMBL
Match: A0A6J1I684 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470013 PE=3 SV=1)

HSP 1 Score: 889 bits (2296), Expect = 0.0
Identity = 462/500 (92.40%), Postives = 463/500 (92.60%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS CQSNCYRNQDDEATVEQDSLRTNK DRLAAT GDSGDTHLCFKCKAKERMSSS
Sbjct: 1   MACNSSACQSNCYRNQDDEATVEQDSLRTNKTDRLAATYGDSGDTHLCFKCKAKERMSSS 60

Query: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIVI 120
           EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR            
Sbjct: 61  EEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSR------------ 120

Query: 121 RILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRTI 180
                   VALQFVHEMQVKAHKNFEASRDRSL VFGVGVAYIDETSACPIPLHEIDRTI
Sbjct: 121 --------VALQFVHEMQVKAHKNFEASRDRSLHVFGVGVAYIDETSACPIPLHEIDRTI 180

Query: 181 QAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240
           Q IQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML
Sbjct: 181 QTIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRML 240

Query: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300
           SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD
Sbjct: 241 SLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLRD 300

Query: 301 CLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360
           CLAQELNMLCCADG            LKTVELTKTSFSGINDLVSSFVALLQEENPSRES
Sbjct: 301 CLAQELNMLCCADG------------LKTVELTKTSFSGINDLVSSFVALLQEENPSRES 360

Query: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420
           TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM
Sbjct: 361 TIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLNM 420

Query: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 480
           SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN
Sbjct: 421 SDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPSN 468

Query: 481 SNSLREQIQEFLLSDDEVES 500
           SNSLRE+IQEFLLSDDEVES
Sbjct: 481 SNSLREKIQEFLLSDDEVES 468

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy TrEMBL
Match: A0A6J1DEW9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111019863 PE=3 SV=1)

HSP 1 Score: 756 bits (1953), Expect = 2.87e-272
Identity = 402/508 (79.13%), Postives = 426/508 (83.86%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-------TNGDSGDTHLCFKCKA 60
           MACNSSTCQSNCYRNQDDE T+EQ S   NKA+  AA       TNGD G+THLCFKCK+
Sbjct: 1   MACNSSTCQSNCYRNQDDEETLEQHSPPANKAEAAAAMESCPVDTNGD-GNTHLCFKCKS 60

Query: 61  KERMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKI 120
            ERMSSS  GDDGRFCS CFRSNLFAKFR AV SNAMISPSDKVLVAFSGGPCSR     
Sbjct: 61  NERMSSSGVGDDGRFCSGCFRSNLFAKFRLAVTSNAMISPSDKVLVAFSGGPCSR----- 120

Query: 121 CSLCIVIRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPL 180
                          VALQFVHE+QVKAHKNFEASRDRSLQVFGVGVAYIDETSAC IP 
Sbjct: 121 ---------------VALQFVHELQVKAHKNFEASRDRSLQVFGVGVAYIDETSACSIPS 180

Query: 181 HEIDRTIQAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDL 240
           HE+D  I+ IQSVVS+L PP K+LHIVQIESVYSSNL D REKLKKLLDTV+DVTGKEDL
Sbjct: 181 HEMDSAIETIQSVVSNLRPPSKELHIVQIESVYSSNLGDRREKLKKLLDTVNDVTGKEDL 240

Query: 241 ISHLRMLSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVP 300
           +SHLRMLSLQKIA ENGYNRL++GTCTSRIA HVISSTVKGQGYSLPADIQYVDARWEVP
Sbjct: 241 LSHLRMLSLQKIATENGYNRLLVGTCTSRIARHVISSTVKGQGYSLPADIQYVDARWEVP 300

Query: 301 VALPLRDCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQE 360
           VALPLRDC+AQEL+MLCC DG            LKTVEL K SFSGIN LVSSFVALLQE
Sbjct: 301 VALPLRDCIAQELHMLCCVDG------------LKTVELAKNSFSGINGLVSSFVALLQE 360

Query: 361 ENPSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAV 420
           ENPSRESTIVRTAGKLTPFNFSRIPDL+ESN VPLATKRRQKRNN KHVNS+SSE+FCAV
Sbjct: 361 ENPSRESTIVRTAGKLTPFNFSRIPDLDESNNVPLATKRRQKRNNVKHVNSISSESFCAV 420

Query: 421 CNGPLNMSDVQTLRSFEAG-QVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVAR 480
           CN PLN+SDVQTLRS E   + ++IL+GT CSSCQFQILPKDS SQEL LSLLPPSMVAR
Sbjct: 421 CNSPLNISDVQTLRSCETSPETANILSGTWCSSCQFQILPKDSTSQELFLSLLPPSMVAR 475

Query: 481 AMHGSPSNSNSLREQIQEFLLSDDEVES 500
           A+HGS  NSNSLREQIQEFLLSDDE ES
Sbjct: 481 AIHGSTGNSNSLREQIQEFLLSDDEAES 475

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy TrEMBL
Match: A0A1S3B7H9 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 PE=3 SV=1)

HSP 1 Score: 735 bits (1898), Expect = 5.00e-264
Identity = 391/505 (77.43%), Postives = 416/505 (82.38%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-----TNGDSGDTHLCFKCKAKE 60
           MACNSS CQSNCYRNQ DE T+EQDS  +++    A      TN D+ +T+LCFKCK+KE
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESRITNDDNSNTNLCFKCKSKE 60

Query: 61  RMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICS 120
           RMS+S  GDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSR       
Sbjct: 61  RMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSR------- 120

Query: 121 LCIVIRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHE 180
                        VALQFVHE+QVKAHKNFEASRDRSLQVFGVGVAYI+ET+A  IP  E
Sbjct: 121 -------------VALQFVHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDE 180

Query: 181 IDRTIQAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLIS 240
           ID+TIQ IQSVVSSLS P KDLHIV IE+VYSSNLSDGREKLKKLLD V DVTGKEDLIS
Sbjct: 181 IDKTIQTIQSVVSSLSQPRKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLIS 240

Query: 241 HLRMLSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVA 300
           +LRMLSLQKIAAENGYNRL++GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV 
Sbjct: 241 YLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVV 300

Query: 301 LPLRDCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEEN 360
           LPL DCLAQELNMLCC DG            LKTVELTK S SGINDLVSSFVALLQ EN
Sbjct: 301 LPLLDCLAQELNMLCCIDG------------LKTVELTKKSSSGINDLVSSFVALLQVEN 360

Query: 361 PSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCN 420
           PSRESTIVRTAGKLTPFNFSRIPDLNESN VPLATKRRQKRNNFKHVNSLSSE+FCAVCN
Sbjct: 361 PSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQKRNNFKHVNSLSSESFCAVCN 420

Query: 421 GPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMH 480
           GPLN SDVQT  +      SS + G CCSSCQFQILPKDS+S EL LSLLPPSMVARA H
Sbjct: 421 GPLNTSDVQTRHA------SSSILGACCSSCQFQILPKDSLSHELFLSLLPPSMVARATH 467

Query: 481 GSPSNSNSLREQIQEFLLSDDEVES 500
           GS  NSNSLR +IQEFLLSDD+ ES
Sbjct: 481 GSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]
BLAST of Cp4.1LG08g13670 vs. ExPASy TrEMBL
Match: A0A5D3DPK4 (Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006240 PE=3 SV=1)

HSP 1 Score: 735 bits (1898), Expect = 5.00e-264
Identity = 391/505 (77.43%), Postives = 416/505 (82.38%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAA-----TNGDSGDTHLCFKCKAKE 60
           MACNSS CQSNCYRNQ DE T+EQDS  +++    A      TN D+ +T+LCFKCK+KE
Sbjct: 1   MACNSSACQSNCYRNQHDEHTIEQDSPPSSRPGTAAVGESRITNDDNSNTNLCFKCKSKE 60

Query: 61  RMSSSEEGDDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICS 120
           RMS+S  GDDGR CSDCFRSNLFAKFRQAV SNAMISPSDKVLVAFSGGPCSR       
Sbjct: 61  RMSTSGVGDDGRLCSDCFRSNLFAKFRQAVTSNAMISPSDKVLVAFSGGPCSR------- 120

Query: 121 LCIVIRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHE 180
                        VALQFVHE+QVKAHKNFEASRDRSLQVFGVGVAYI+ET+A  IP  E
Sbjct: 121 -------------VALQFVHEIQVKAHKNFEASRDRSLQVFGVGVAYINETAAYSIPSDE 180

Query: 181 IDRTIQAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLIS 240
           ID+TIQ IQSVVSSLS P KDLHIV IE+VYSSNLSDGREKLKKLLD V DVTGKEDLIS
Sbjct: 181 IDKTIQTIQSVVSSLSQPRKDLHIVPIENVYSSNLSDGREKLKKLLDAVDDVTGKEDLIS 240

Query: 241 HLRMLSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVA 300
           +LRMLSLQKIAAENGYNRL++GTCTSRIACHVISSTVKGQGYSLPAD+QY+DARWEVPV 
Sbjct: 241 YLRMLSLQKIAAENGYNRLLVGTCTSRIACHVISSTVKGQGYSLPADVQYIDARWEVPVV 300

Query: 301 LPLRDCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEEN 360
           LPL DCLAQELNMLCC DG            LKTVELTK S SGINDLVSSFVALLQ EN
Sbjct: 301 LPLLDCLAQELNMLCCIDG------------LKTVELTKKSSSGINDLVSSFVALLQVEN 360

Query: 361 PSRESTIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCN 420
           PSRESTIVRTAGKLTPFNFSRIPDLNESN VPLATKRRQKRNNFKHVNSLSSE+FCAVCN
Sbjct: 361 PSRESTIVRTAGKLTPFNFSRIPDLNESNNVPLATKRRQKRNNFKHVNSLSSESFCAVCN 420

Query: 421 GPLNMSDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMH 480
           GPLN SDVQT  +      SS + G CCSSCQFQILPKDS+S EL LSLLPPSMVARA H
Sbjct: 421 GPLNTSDVQTRHA------SSSILGACCSSCQFQILPKDSLSHELFLSLLPPSMVARATH 467

Query: 481 GSPSNSNSLREQIQEFLLSDDEVES 500
           GS  NSNSLR +IQEFLLSDD+ ES
Sbjct: 481 GSLGNSNSLRNKIQEFLLSDDDAES 467

[Close]
BLAST of Cp4.1LG08g13670 vs. TAIR 10
Match: AT4G35910.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-144
Identity = 275/498 (55.22%), Postives = 343/498 (68.88%), Query Frame = 0 

Query: 1   MACNSSTCQSNCYRNQDDEATVEQDSLRTNKADRLAATNGDSGDTHLCFKCKAKERMSSS 60
           MACNSS C+S CY  + D  +   D           A +G      +C KCK    M+  
Sbjct: 1   MACNSSGCESGCYDREKDNGSKIVDD----------AVSGGGNHESVCVKCKCNAPMTFG 60

Query: 61  EEG-DDGRFCSDCFRSNLFAKFRQAVNSNAMISPSDKVLVAFSGGPCSRLVQKICSLCIV 120
           + G DDGRFC+DCFR+N+F KFR AV S+AMI+PSD VLVAFSGG               
Sbjct: 61  DGGFDDGRFCADCFRNNVFGKFRLAVTSHAMITPSDNVLVAFSGG--------------- 120

Query: 121 IRILEQASRVALQFVHEMQVKAHKNFEASRDRSLQVFGVGVAYIDETSACPIPLHEIDRT 180
                 +SRV+LQFVHE+Q+KA KN+EASRDRSL VFGVGVA++DET+A P    E+   
Sbjct: 121 -----SSSRVSLQFVHELQIKALKNYEASRDRSLPVFGVGVAFVDETAAFPALSTEMIDA 180

Query: 181 IQAIQSVVSSLSPPIKDLHIVQIESVYSSNLSDGREKLKKLLDTVSDVTGKEDLISHLRM 240
           I+ ++  VS LSPP KDLH+V +ES++ S+  D R++L KLLD+V D TGKEDL+ HL+M
Sbjct: 181 IEWVRYTVSCLSPPAKDLHVVPVESIFGSDSLDARDRLLKLLDSVPDDTGKEDLLLHLKM 240

Query: 241 LSLQKIAAENGYNRLVLGTCTSRIACHVISSTVKGQGYSLPADIQYVDARWEVPVALPLR 300
           LSLQK+AAENGYNRLVLG+CTSRIA HV+++TVKG+GYSL ADIQ+VDARW+VP+ LPLR
Sbjct: 241 LSLQKVAAENGYNRLVLGSCTSRIASHVLTATVKGRGYSLSADIQHVDARWKVPIVLPLR 300

Query: 301 DCLAQELNMLCCADGSVFVRSGSWQGSLKTVELTKTSFSGINDLVSSFVALLQEENPSRE 360
           DC+  E+  LC  DG            LKTVEL   S  GINDLVSSFVALLQEENPSRE
Sbjct: 301 DCVRLEITRLCLLDG------------LKTVELACRSQCGINDLVSSFVALLQEENPSRE 360

Query: 361 STIVRTAGKLTPFNFSRIPDLNESNGVPLATKRRQKRNNFKHVNSLSSETFCAVCNGPLN 420
            TIVRTA KLTPF F++IP+ ++SN VP+AT+RR KR N K+  S+++E FC +CNGPLN
Sbjct: 361 CTIVRTAAKLTPFYFNKIPETDDSN-VPMATQRRLKRFNLKYDGSMTTEAFCPICNGPLN 420

Query: 421 MSDVQTLRSFEAGQVSSILAGTCCSSCQFQILPKDSISQELLLSLLPPSMVARAMHGSPS 480
            SD   L +FE GQ S +L   CCSSC+FQILP+D  S E   S LP  M+++  H    
Sbjct: 421 RSDSSELDTFEEGQESDVLYAACCSSCRFQILPQDGSSLEQFSSFLPDHMISQVKHQKVD 455

Query: 481 NSNSLREQIQEFLLSDDE 498
           +   LRE+I++ LL DDE
Sbjct: 481 SQAYLREKIKDCLLLDDE 455

The following BLAST results are available for this feature:
BLAST of Cp4.1LG08g13670 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O656283.1e-14355.22Cytoplasmic tRNA 2-thiolation protein 2 OS=Arabidopsis thaliana OX=3702 GN=CTU2 ... [more]
Q2QMW07.1e-10042.97Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. japonica OX=39947... [more]
A2ZM731.6e-9942.97Cytoplasmic tRNA 2-thiolation protein 2 OS=Oryza sativa subsp. indica OX=39946 G... [more]
Q08B128.1e-1924.54Cytoplasmic tRNA 2-thiolation protein 2-A OS=Xenopus laevis OX=8355 GN=ctu2-a PE... [more]
Q2VPK54.4e-1725.06Cytoplasmic tRNA 2-thiolation protein 2 OS=Homo sapiens OX=9606 GN=CTU2 PE=1 SV=... [more]
BLAST of Cp4.1LG08g13670 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023539180.10.093.60cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita pepo subsp. pepo][more]
KAG7027733.10.093.95Cytoplasmic tRNA 2-thiolation protein 2, partial [Cucurbita argyrosperma subsp. ... [more]
XP_022939604.10.093.00cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita moschata][more]
XP_022971223.10.092.40cytoplasmic tRNA 2-thiolation protein 2 [Cucurbita maxima][more]
KAG6596194.12.60e-29686.60Cytoplasmic tRNA 2-thiolation protein 2, partial [Cucurbita argyrosperma subsp. ... [more]
BLAST of Cp4.1LG08g13670 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FHP70.093.00Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1I6840.092.40Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111470... [more]
A0A6J1DEW92.87e-27279.13Cytoplasmic tRNA 2-thiolation protein 2 OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A1S3B7H95.00e-26477.43Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo OX=3656 GN=LOC103486848 ... [more]
A0A5D3DPK45.00e-26477.43Cytoplasmic tRNA 2-thiolation protein 2 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
BLAST of Cp4.1LG08g13670 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G35910.12.2e-14455.22Adenine nucleotide alpha hydrolases-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019407Cytoplasmic tRNA 2-thiolation protein 2PFAMPF10288CTU2coord: 340..436
e-value: 3.6E-7
score: 30.4
IPR019407Cytoplasmic tRNA 2-thiolation protein 2PANTHERPTHR20882CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2coord: 42..494
IPR019407Cytoplasmic tRNA 2-thiolation protein 2HAMAPMF_03054CTU2coord: 42..454
score: 19.554924
IPR014729Rossmann-like alpha/beta/alpha sandwich foldGENE3D3.40.50.620HUPscoord: 47..435
e-value: 2.1E-35
score: 124.3
NoneNo IPR availableSUPERFAMILY52402Adenine nucleotide alpha hydrolases-likecoord: 71..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g13670.1Cp4.1LG08g13670.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010311 lateral root formation
biological_process GO:0032447 protein urmylation
biological_process GO:0002143 tRNA wobble position uridine thiolation
biological_process GO:0034227 tRNA thio-modification
biological_process GO:0002098 tRNA wobble uridine modification
cellular_component GO:0005829 cytosol
molecular_function GO:0016779 nucleotidyltransferase activity
molecular_function GO:0016783 sulfurtransferase activity
molecular_function GO:0000049 tRNA binding