Cp4.1LG08g13540 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g13540
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionTFIIS N-terminal domain-containing protein
LocationCp4.1LG08: 9723766 .. 9727982 (-)
RNA-Seq ExpressionCp4.1LG08g13540
SyntenyCp4.1LG08g13540
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAAAGAAAAAAGAAGGTGTAGCGTGAGAGGAAGGGGAGAGAGAGAGAGGAGTTTCGTGGGTTTGGAGCAAAAAACAAAAGAAAAAAAAAGGAAAGATTTTTATGAATGTTGAGGGGAGCCTAAACCCAGGTTTATTCTTTTAATTTCTTTTTAATTTCTTTCTAATTTCTTTCTAATTTCTTTATAATTTCTAGAGAGAGAAACAAATCAAAGGAACCCTTCAATTCCCAATTCCCAATTCCAATTTCAATTTCAATTTCAATTCCCAATTCCCAATTCCATTGAAGAGAAAGAAAAGATTAAGGTTTTGTTCCTATTTTCTCTTCTCTTCCATTTCCCATATTCTCGATATTTCTCCTACCAGGAAATCCCGGAACTTTTCCGGGATTAAACAAATACGAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCAGAAGTTTCTCTGTTACAACCTTCCAATTTTGAACAACCCCTCATTGCAGGGCTTGGGTTTTATCAATCCCCTTCTTCTGTTTTTGATCCGATCCTTTCTTTTCACAAAACGAATATCTTCACACCACTACTCACTTTCATTCGCTTCCGAACGGATGCAAGTTTGAGTGTTTGCGACTTTGTTGCTAACCATTTTTATTTTTCTGGGAATCGGATTCTTGTGGTGCTGGATTAGTGTTGGGGAGGGGTTTTCTTTTCTGGGTGTTGTTCATTTTGGGGGATTTCGTCAATACCCACCTCCAATCTTTGTTTGTTTTTCTTCGTATTTAGGTTTTTCTTCTTTGGAACTCTCACGGTGATGGAATTCCCACTTCGATTCTGAGTGGTTGTTTGAATTCCGTCCGTGTTCGATCGAGTTTGGTTTCTTCAGATTCCGATCATGTCCGCCAAGTCTGCGTCCTTGGATTACTGGAGGGATTACTTCCGGAATGCGAATTCGGATATTTTTAGCTTAATCGATTACGCAATCTTGGTGGCGGCTTCCGATTGTCCCAAGGAGTTCAGATTGAGGAGAGATCGAATTGCAGAGCAGTTGTTTTCATGCCGATTGACTCGCTGTTTGGGTTGCGACCGAGTGGAGCTGGCGGTGGCCGGCGATGTGGACGGCGAAACGGGTTTCAAGAGCGATTCTGCCGGAGATGGGCGTGCGTTTGAGGGCGGTGGGAGCAAAGAGAGCAAGGTTTACAGCAGTAGAGATGATGGAGGGGAGATGAATTTCAATCAGGTTAGCAATTATAGCTTTGGTGAAGCTGAAGCATTGACTGATGAAATTGAACAAGAATCTGAGATTGTTGGGGAGGTTTTGAGGATCAAAGAGATTCTCAATAATTTTGAAGAAGAGGTAATGAATCATTGTTTTGGGTCTTTACATTTTCTGGGATGTTCCTTTGTTTCTCATGTTGTGTGTTTCATGTGCAGTCTGATTCAGTGTTGTTTGAATCCCTTAGAAGACTTGAATTGATGGCTCTATCAGTTGATGCCTTGCAGGTGATGATTTGAATTCTTCTGTTCGCTCTTGCAGCTTTGGGGCAAGTTAAGTTTTGTTGGTTGTTGTTCTTGTTTCTGATTGAGGATTTTTCTCTCTTTTTCAGGCAACTGAGATTGGCAAGGCTGTTAATGGTCTTCGAAAGCATGGATCGAAGCGCATTCGCCATCTTGTTCGATTTCTTATCACGTACTAAATCTTGCCTTCAAACCTCAATACCAAGTATACATGTTCTATGATGAGTGCCCCAACTAAATTTGTTGTTGACACTCTATATGTCATCTTCCTCTGGATTTGTTCTTTAAAACTTACTCATTTCGTTTTGAACATACAAGAATCAGTGTTTGTTCTCTCTTTTGACTGAGTTTTAATGAACAAAGAATCGTCTTCTTCGACCATGCAGCGTCCATTATTTTGTTTTTTGAGTGAGTGGAGAGCTTTTGTAGCTCATCTATGCCTTTACAACTTGTTGCTCACTCAGGGAGTTCTCTGTTTTGCATAGCATATAAGGAAACCTCGTCTTTGATATCATTATAAATGGCTGCTTGTTCTCAGTTTACTTTGCTTGAGGCTAAAAGATCTACTTTATTGGTTGCAGAGAGTGGAAAGAAATGGTGGACATGTGGGTAAAAGCTACAGCTGCTGTTCATGGTAGAGTTCATTTTAGCTACACGTGCATCTATATCGTTTCATGCCAACTTTCGAGATTTATTTACTGAGGAATGTTTTTGTTTTCCTGAATAAAAAGGTTCAGAAGTCACACCGGATTCGAAAAGTATGCCTGCATCTGAACAAGAAGAAGATGATTATGAAGATGGGCTTCCCTCTCCTCCTTTAGATGAAGCTGCATTTTTTACCACTCAGCCCACATCAATGGAGCTCTCACAAGTATGTCTCTATTTTCGAGTTGAATTGAGTTTGTATTCTCAAGAAAAGTTATCTACTTTATGTTGCATTGGTGGGTTCTAATCGACTGTATTTTACTTGCCATTCCATTCCCTTCCTTGGCGAAGTTCTTTGATGGCATGGACGATGATGGAAGTGAGTTCATTATGCAATATCCTATGCTTTTCTCAATCACTTTTTTAGACTCTCCATTTCTTAGATTCATGATTACCAATGGGGATATATGTTTCAGATCCTCGAAACAGTGGAGAGTTCGTCAAGAAGCACGATAATGGAAGAAAAACATCGAATGGCAGTCAAAACCCTATGAGGTGTAAACAACAAACCACTCGAGAGGTTAATGTGGTTGCTAAGGACAATAAGAGTCAAATGAAAGAACAAGTTGTGAAGCCAAACAAGCCACCGTCAAATGCAAATTCTGGGCTTTCGAGGCCGCAGAAGCCGAGCAGCGTGCAGAAGGACAATCATGAGAAGTTGCAGCGTAAAGTGGAGAAGCCTACGATCCCAAAGAGGAGCCTGCAAGATGTAAGTAGCTGAAGATTGTAAAGTTATTTGGGGGTTTGTCCTTTGTCCTTGTACCTGATTTTTTCGATTTGTGTGTACCAGAAGTTCAAGTGTTCGGATGAGGTTGCAGTTCAAGTGAAACTAGAAGCCACTAAGAGGAAACTGCAAGAGAGTTACCAACAAGCTGAAAACGGTATTTGCTTTGTTTTGTTTTGTTTTCCATCTATGCTTTTATCCATTCTGATACCAGTTTCATAGGCAGTCTAAAACCTTTCTTGCTGAATATTTTAAGTATCAATCAAAGCAACTTTAGCTAAATATTTGCACCCGACCATAGTTTAACTGGTTAAGGCGTATATGACCAAAATGGGAGCTCTTTTAAACACATTTATGAAGTTGTTGAATATAACTAACTACATAAGTGCTGATTCATGGTAGTGTTTATTGAACAGCCAAAAAGCAGCGGACGATACAGGTTATGGAACTACACGATATTCCGAAGCAGGGAATGGGCCACAGAAATGTGCACCTAAAATCTGGAGGCCACAATCGGCATTGGACAAACGGGAGGCGTTAGGATGAAACGATAGTGAGGAACAAGACTGATACTTCCAGGATACTTTCCTGGTGACTTTCCACCCAGAATCAGGTGGAAGATCATACTAAAAGCTCCCTGGCAAACGGTGTCTTGACCTACTCTACCTGCTTCAAGGTAAACAACAAGAAACTTGGTTTCACTGTGTTGTGCCAGAGAAGGCGATTCCGATTCAAAGGAGAATCGGAGATGGAAAAAAAAAAAAAAGGAGAAAAAAAAAGAAAGAACGATTTCGTAGTTGAAGTACATTTTGAAGTATAGAGAATTTCTTATGGAATTTGGGAGGCAAAATTATATAGCTTTTATCGGTGGTTGCTCAGGGAGTGTTGACATGGTTATATCTCTCTTCTTACATCATAATGACTGTATAGAACCCCCTCATTTATTTTGGTTTCAAATTTTGCAAGCTATTCTCTCTCTCTCTTCTCTTCTCTTGCTTGCTTGCTAGGAACTGAGGAGCTTCTGGTTGTGATGGTCCTTTTGGCTATTATGACCCCACCCAAAGCCGAACCTAATTAATCAACCAAATCCAATTCCAAAAGCCAACCTCCTTTTTCAACGTTTTGTACTGTGTGTTCGTCTCTCAAAACTGTGCCTTTTGGGACTCTGCTCTGCCCTGCTCTGCTGTGAGGGGAGGGAGCATCATGGGGCCACTCACCTTTTCATCTCTTTATTATAATCTATCAATAATATTTATATTTC

mRNA sequence

AGAGAAAGAAAAAAGAAGGTGTAGCGTGAGAGGAAGGGGAGAGAGAGAGAGGAGTTTCGTGGGTTTGGAGCAAAAAACAAAAGAAAAAAAAAGGAAAGATTTTTATGAATGTTGAGGGGAGCCTAAACCCAGGTTTATTCTTTTAATTTCTTTTTAATTTCTTTCTAATTTCTTTCTAATTTCTTTATAATTTCTAGAGAGAGAAACAAATCAAAGGAACCCTTCAATTCCCAATTCCCAATTCCAATTTCAATTTCAATTTCAATTCCCAATTCCCAATTCCATTGAAGAGAAAGAAAAGATTAAGGTTTTGTTCCTATTTTCTCTTCTCTTCCATTTCCCATATTCTCGATATTTCTCCTACCAGGAAATCCCGGAACTTTTCCGGGATTAAACAAATACGAANAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCAGAAGTTTCTCTGTTACAACCTTCCAATTTTGAACAACCCCTCATTGCAGGGCTTGGGTTTTATCAATCCCCTTCTTCTGTTTTTGATCCGATCCTTTCTTTTCACAAAACGAATATCTTCACACCACTACTCACTTTCATTCGCTTCCGAACGGATGCAAGTTTGAGTGTTTGCGACTTTGTTGCTAACCATTTTTATTTTTCTGGGAATCGGATTCTTGTGGTGCTGGATTAGTGTTGGGGAGGGGTTTTCTTTTCTGGGTGTTGTTCATTTTGGGGGATTTCGTCAATACCCACCTCCAATCTTTGTTTGTTTTTCTTCGTATTTAGGTTTTTCTTCTTTGGAACTCTCACGGTGATGGAATTCCCACTTCGATTCTGAGTGGTTGTTTGAATTCCGTCCGTGTTCGATCGAGTTTGGTTTCTTCAGATTCCGATCATGTCCGCCAAGTCTGCGTCCTTGGATTACTGGAGGGATTACTTCCGGAATGCGAATTCGGATATTTTTAGCTTAATCGATTACGCAATCTTGGTGGCGGCTTCCGATTGTCCCAAGGAGTTCAGATTGAGGAGAGATCGAATTGCAGAGCAGTTGTTTTCATGCCGATTGACTCGCTGTTTGGGTTGCGACCGAGTGGAGCTGGCGGTGGCCGGCGATGTGGACGGCGAAACGGGTTTCAAGAGCGATTCTGCCGGAGATGGGCGTGCGTTTGAGGGCGGTGGGAGCAAAGAGAGCAAGGTTTACAGCAGTAGAGATGATGGAGGGGAGATGAATTTCAATCAGGTTAGCAATTATAGCTTTGGTGAAGCTGAAGCATTGACTGATGAAATTGAACAAGAATCTGAGATTGTTGGGGAGGTTTTGAGGATCAAAGAGATTCTCAATAATTTTGAAGAAGAGTCTGATTCAGTGTTGTTTGAATCCCTTAGAAGACTTGAATTGATGGCTCTATCAGTTGATGCCTTGCAGGCAACTGAGATTGGCAAGGCTGTTAATGGTCTTCGAAAGCATGGATCGAAGCGCATTCGCCATCTTGTTCGATTTCTTATCACAGAGTGGAAAGAAATGGTGGACATGTGGGTAAAAGCTACAGCTGCTGTTCATGGTTCAGAAGTCACACCGGATTCGAAAAGTATGCCTGCATCTGAACAAGAAGAAGATGATTATGAAGATGGGCTTCCCTCTCCTCCTTTAGATGAAGCTGCATTTTTTACCACTCAGCCCACATCAATGGAGCTCTCACAATTCTTTGATGGCATGGACGATGATGGAAATCCTCGAAACAGTGGAGAGTTCGTCAAGAAGCACGATAATGGAAGAAAAACATCGAATGGCAGTCAAAACCCTATGAGGTGTAAACAACAAACCACTCGAGAGGTTAATGTGGTTGCTAAGGACAATAAGAGTCAAATGAAAGAACAAGTTGTGAAGCCAAACAAGCCACCGTCAAATGCAAATTCTGGGCTTTCGAGGCCGCAGAAGCCGAGCAGCGTGCAGAAGGACAATCATGAGAAGTTGCAGCGTAAAGTGGAGAAGCCTACGATCCCAAAGAGGAGCCTGCAAGATAAGTTCAAGTGTTCGGATGAGGTTGCAGTTCAAGTGAAACTAGAAGCCACTAAGAGGAAACTGCAAGAGAGTTACCAACAAGCTGAAAACGCCAAAAAGCAGCGGACGATACAGGTTATGGAACTACACGATATTCCGAAGCAGGGAATGGGCCACAGAAATGTGCACCTAAAATCTGGAGGCCACAATCGGCATTGGACAAACGGGAGGCGTTAGGATGAAACGATAGTGAGGAACAAGACTGATACTTCCAGGATACTTTCCTGGTGACTTTCCACCCAGAATCAGGTGGAAGATCATACTAAAAGCTCCCTGGCAAACGGTGTCTTGACCTACTCTACCTGCTTCAAGGTAAACAACAAGAAACTTGGTTTCACTGTGTTGTGCCAGAGAAGGCGATTCCGATTCAAAGGAGAATCGGAGATGGAAAAAAAAAAAAAAGGAGAAAAAAAAAGAAAGAACGATTTCGTAGTTGAAGTACATTTTGAAGTATAGAGAATTTCTTATGGAATTTGGGAGGCAAAATTATATAGCTTTTATCGGTGGTTGCTCAGGGAGTGTTGACATGGTTATATCTCTCTTCTTACATCATAATGACTGTATAGAACCCCCTCATTTATTTTGGTTTCAAATTTTGCAAGCTATTCTCTCTCTCTCTTCTCTTCTCTTGCTTGCTTGCTAGGAACTGAGGAGCTTCTGGTTGTGATGGTCCTTTTGGCTATTATGACCCCACCCAAAGCCGAACCTAATTAATCAACCAAATCCAATTCCAAAAGCCAACCTCCTTTTTCAACGTTTTGTACTGTGTGTTCGTCTCTCAAAACTGTGCCTTTTGGGACTCTGCTCTGCCCTGCTCTGCTGTGAGGGGAGGGAGCATCATGGGGCCACTCACCTTTTCATCTCTTTATTATAATCTATCAATAATATTTATATTTC

Coding sequence (CDS)

ATGTCCGCCAAGTCTGCGTCCTTGGATTACTGGAGGGATTACTTCCGGAATGCGAATTCGGATATTTTTAGCTTAATCGATTACGCAATCTTGGTGGCGGCTTCCGATTGTCCCAAGGAGTTCAGATTGAGGAGAGATCGAATTGCAGAGCAGTTGTTTTCATGCCGATTGACTCGCTGTTTGGGTTGCGACCGAGTGGAGCTGGCGGTGGCCGGCGATGTGGACGGCGAAACGGGTTTCAAGAGCGATTCTGCCGGAGATGGGCGTGCGTTTGAGGGCGGTGGGAGCAAAGAGAGCAAGGTTTACAGCAGTAGAGATGATGGAGGGGAGATGAATTTCAATCAGGTTAGCAATTATAGCTTTGGTGAAGCTGAAGCATTGACTGATGAAATTGAACAAGAATCTGAGATTGTTGGGGAGGTTTTGAGGATCAAAGAGATTCTCAATAATTTTGAAGAAGAGTCTGATTCAGTGTTGTTTGAATCCCTTAGAAGACTTGAATTGATGGCTCTATCAGTTGATGCCTTGCAGGCAACTGAGATTGGCAAGGCTGTTAATGGTCTTCGAAAGCATGGATCGAAGCGCATTCGCCATCTTGTTCGATTTCTTATCACAGAGTGGAAAGAAATGGTGGACATGTGGGTAAAAGCTACAGCTGCTGTTCATGGTTCAGAAGTCACACCGGATTCGAAAAGTATGCCTGCATCTGAACAAGAAGAAGATGATTATGAAGATGGGCTTCCCTCTCCTCCTTTAGATGAAGCTGCATTTTTTACCACTCAGCCCACATCAATGGAGCTCTCACAATTCTTTGATGGCATGGACGATGATGGAAATCCTCGAAACAGTGGAGAGTTCGTCAAGAAGCACGATAATGGAAGAAAAACATCGAATGGCAGTCAAAACCCTATGAGGTGTAAACAACAAACCACTCGAGAGGTTAATGTGGTTGCTAAGGACAATAAGAGTCAAATGAAAGAACAAGTTGTGAAGCCAAACAAGCCACCGTCAAATGCAAATTCTGGGCTTTCGAGGCCGCAGAAGCCGAGCAGCGTGCAGAAGGACAATCATGAGAAGTTGCAGCGTAAAGTGGAGAAGCCTACGATCCCAAAGAGGAGCCTGCAAGATAAGTTCAAGTGTTCGGATGAGGTTGCAGTTCAAGTGAAACTAGAAGCCACTAAGAGGAAACTGCAAGAGAGTTACCAACAAGCTGAAAACGCCAAAAAGCAGCGGACGATACAGGTTATGGAACTACACGATATTCCGAAGCAGGGAATGGGCCACAGAAATGTGCACCTAAAATCTGGAGGCCACAATCGGCATTGGACAAACGGGAGGCGTTAG

Protein sequence

MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRCLGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYSFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATEIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEEDDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGSQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR
Homology
BLAST of Cp4.1LG08g13540 vs. ExPASy Swiss-Prot
Match: Q9FHK9 (Probable mediator of RNA polymerase II transcription subunit 26b OS=Arabidopsis thaliana OX=3702 GN=MED26B PE=2 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 6.9e-86
Identity = 214/462 (46.32%), Postives = 280/462 (60.61%), Query Frame = 0

Query: 5   SASLDYWRDYF-RNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRCLGC 64
           S SLD WR+YF R  +SDIF +ID+AI+VAA+DCP +F+ RRD+IAE LFSCR+ RC+GC
Sbjct: 4   SGSLDSWREYFRRRGDSDIFGIIDHAIMVAATDCPNKFKSRRDKIAELLFSCRVNRCVGC 63

Query: 65  DRVELAVAGDVDGETGFKSDSAGDGRAFE----GGGSKESKVYSSRDDGGEMNFNQVSNY 124
           D +EL+V GD +   G   +  G     E     GGSKESK  SSR D  ++    VSNY
Sbjct: 64  DHLELSVPGDDEANRGTTGNGGGGTAVDEDYEVAGGSKESKANSSRGDNNQI----VSNY 123

Query: 125 SFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQAT 184
           +F EAEAL+DEIE+ S +  EV RIKEIL N E+E +SVL +SLR L+LM+L+VD L++T
Sbjct: 124 TFDEAEALSDEIEEFSVVSKEVARIKEILLNKEDEPNSVLLDSLRHLKLMSLNVDILKST 183

Query: 185 EIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQE 244
           EIGKAVNGLRKH S +IR L + LI EWKE+VD WV  T  + G+E TP+S +    ++E
Sbjct: 184 EIGKAVNGLRKHSSDKIRQLAKTLIAEWKELVDQWVNTTKEITGAEGTPESANPSVLDEE 243

Query: 245 EDDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNG 304
           E       PS P D    FT +P   E+S FFD +D DGNPRNS E    H+  R+    
Sbjct: 244 E-----AFPSLPYD-VDIFTPEPNGFEISHFFDSLDFDGNPRNSEE----HNTSREHERR 303

Query: 305 SQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNAN-SGLSRPQKPSSVQKDNHE 364
            QN  + K + T          + ++++   +  KP S  +  G  RP K S+ Q+  +E
Sbjct: 304 PQNIAKRKPEGT----------QMRIQDAPFRSIKPSSATDFDGTRRPVKQSTEQRMKNE 363

Query: 365 KLQ-RKVEKPTIPKRSL------------QDKFKCSDEVAVQVKLEATKRKLQESYQQAE 424
            +   K EKP I ++ +            Q+K K  D      K E  KRKLQESYQ  E
Sbjct: 364 TVSVHKSEKPMIQRKPVVTEQKRKAPGPQQEKLKGLD---ADAKFEFAKRKLQESYQHHE 423

Query: 425 NAKKQRTIQVMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 448
           NAKKQRTIQV+E+  IPKQG   +    + G  NR+W NGR+
Sbjct: 424 NAKKQRTIQVLEM--IPKQGSAQKPQLKRPGMSNRNWANGRK 436

BLAST of Cp4.1LG08g13540 vs. ExPASy Swiss-Prot
Match: F4J4Y5 (Probable mediator of RNA polymerase II transcription subunit 26a OS=Arabidopsis thaliana OX=3702 GN=MED26A PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.5e-66
Identity = 178/428 (41.59%), Postives = 249/428 (58.18%), Query Frame = 0

Query: 5   SASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRCLGCD 64
           S SLD WR+YFR  +SDIF +ID+AI+VAA+D PKEF+ R DRIAE LFSC+++RC+GCD
Sbjct: 7   SVSLDTWREYFRRGDSDIFGIIDHAIMVAAADWPKEFKSRSDRIAELLFSCKVSRCIGCD 66

Query: 65  RVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYSFGEA 124
            +EL++AGD         ++A +     GGG +                        G++
Sbjct: 67  HLELSIAGD---------EAAVEIVGVGGGGDR------------------------GDS 126

Query: 125 EALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATEIGKA 184
              T E E+ S  V EV+RI++IL+N ++E DSVL ESLR+LE M++SVD L+ TEIGKA
Sbjct: 127 GVATGEGEEASVSVDEVMRIRDILSNKDDEKDSVLLESLRKLESMSMSVDILKDTEIGKA 186

Query: 185 VNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEEDDYE 244
           VNGLR+H S +I  L + L  EWK +VD W+     + G+E TP+S ++   ++EE    
Sbjct: 187 VNGLRRHSSDKISKLAKTLFAEWKRLVDQWMNTPEEMAGTEGTPESLNLSVIDEEE---- 246

Query: 245 DGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGSQNPM 304
              PSPP D    +  +P   ELSQ  D +D DGNPR+S E   KH+   ++S G     
Sbjct: 247 -AFPSPPHD-LDIYAPEPNGFELSQILDCLDCDGNPRHSVE--SKHERKSQSSAGR---- 306

Query: 305 RCKQQTTREVNVVAK---DNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNH-EKL 364
             + + T + NVV +   D +++ +E  V+P K   ++ + +  P++ +   ++     +
Sbjct: 307 --RPKGTNDANVVGRYCNDQQTRREEADVRPMK---HSATDVVEPKRQTKQSREQMVSAI 366

Query: 365 QRKVEKPTIPKRSL----QDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVM 424
           QRK    T  KR L    QDK K  D      K E  KRKLQESY Q ENAK+QRTIQV+
Sbjct: 367 QRKPTAVTEQKRKLAGPQQDKLKALDP---DSKFEFAKRKLQESYHQHENAKRQRTIQVL 379

BLAST of Cp4.1LG08g13540 vs. ExPASy Swiss-Prot
Match: F4KFC7 (Probable mediator of RNA polymerase II transcription subunit 26c OS=Arabidopsis thaliana OX=3702 GN=MED26C PE=1 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 1.7e-20
Identity = 124/442 (28.05%), Postives = 194/442 (43.89%), Query Frame = 0

Query: 8   LDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRL-TRCLGCDRV 67
           LD +R    NA  D+++ ID AILVA+ D  +E + RRD I E+L++  +  +C  CD  
Sbjct: 3   LDDFRSVMDNAGVDVWTFIDTAILVASLDYGQELKRRRDNIVERLYATSMANKCRNCD-- 62

Query: 68  ELAVAGDVDGETGFKSDSAGDGRAFE--GGGSKESKVYSSRDDGGEMNFNQVSNYSFGEA 127
                G     T        +GR  E      +E    ++ ++  E + N   +  F   
Sbjct: 63  ----FGGGGNVTEAAIGRVNNGRVHEETEEEDEEGVTAAAEEEVREKSVNVEDDDDFDPF 122

Query: 128 EALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATEIGKA 187
             L D+ EQ+S     ++ IKE L +  + S+  L E L+ LE M ++  ALQ T+IG+ 
Sbjct: 123 AGLFDD-EQKS-----IVEIKEKLED-PDLSEESLVELLQNLEDMDITFQALQETDIGRH 182

Query: 188 VNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEEDDYE 247
           VN +RKH S  +R L + L+ +WKE VD WVK           P     P+   +ED   
Sbjct: 183 VNRVRKHPSNNVRRLAKQLVKKWKETVDEWVKFN--------QPGDLEPPSLIADEDS-- 242

Query: 248 DGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGSQNPM 307
                 P+ +A                     +G+ +   +F             S  P 
Sbjct: 243 ------PVQKAL-------------------HNGSRQQVPDF-----------GYSPVPQ 302

Query: 308 RCKQQTTREVNVVAKDNKSQMKEQVVKPNKP-PSNANSGLSRPQKPSSVQKDNHEKLQRK 367
                +++  N+   + K        +P  P P   +   ++P +PS  Q+         
Sbjct: 303 NGYSSSSKNSNITEPERKP-------RPVAPQPRRESPSPAKPSRPSPSQQ--------- 353

Query: 368 VEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHDIPK 427
               TIP+          D+   +V  +  +++LQ++Y+QAENAKKQRTIQVM++HDIPK
Sbjct: 363 ----TIPR----------DKEHKEVDFDTARKRLQQNYRQAENAKKQRTIQVMDIHDIPK 353

Query: 428 QGMGHRNVHLKSGGHN---RHW 443
              G      + GG +   RHW
Sbjct: 423 PKKG--GFFPRKGGSSQGGRHW 353

BLAST of Cp4.1LG08g13540 vs. NCBI nr
Match: XP_023539160.1 (probable mediator of RNA polymerase II transcription subunit 26b [Cucurbita pepo subsp. pepo])

HSP 1 Score: 854 bits (2207), Expect = 5.19e-312
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120
           LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS
Sbjct: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120

Query: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180
           FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE
Sbjct: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180

Query: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240
           IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE
Sbjct: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240

Query: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300
           DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS
Sbjct: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300

Query: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKL 360
           QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKL
Sbjct: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKL 360

Query: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420
           QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD
Sbjct: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420

Query: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           IPKQGMGHRNVHLKSGGHNRHWTNGRR
Sbjct: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447

BLAST of Cp4.1LG08g13540 vs. NCBI nr
Match: KAG6596180.1 (putative mediator of RNA polymerase II transcription subunit 26b, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027717.1 putative mediator of RNA polymerase II transcription subunit 26b [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 840 bits (2170), Expect = 2.26e-306
Identity = 440/447 (98.43%), Postives = 443/447 (99.11%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           MSAKS SLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MSAKSGSLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120
           LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS
Sbjct: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120

Query: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180
           FGEAEALTDEIEQ+SEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE
Sbjct: 121 FGEAEALTDEIEQQSEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180

Query: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240
           IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE
Sbjct: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240

Query: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300
           DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKK DNGRKTS+GS
Sbjct: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKRDNGRKTSSGS 300

Query: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKL 360
           QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSG SR QKPS+VQKDNHEKL
Sbjct: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGFSRLQKPSTVQKDNHEKL 360

Query: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420
           QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD
Sbjct: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420

Query: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           IPKQGMGHRNVHLKSGGHNRHWTNGRR
Sbjct: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447

BLAST of Cp4.1LG08g13540 vs. NCBI nr
Match: XP_022936188.1 (probable mediator of RNA polymerase II transcription subunit 26b [Cucurbita moschata])

HSP 1 Score: 840 bits (2169), Expect = 3.21e-306
Identity = 440/447 (98.43%), Postives = 442/447 (98.88%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           MSAKS SLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MSAKSGSLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120
           LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS
Sbjct: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120

Query: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180
           FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE
Sbjct: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180

Query: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240
           IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMP SEQEE
Sbjct: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPVSEQEE 240

Query: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300
           DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKK DNGRKTS+GS
Sbjct: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKRDNGRKTSSGS 300

Query: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKL 360
           QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSG SR QKPS+VQKDNHEKL
Sbjct: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGFSRLQKPSTVQKDNHEKL 360

Query: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420
           QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD
Sbjct: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420

Query: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           IPKQGMGHRNVHLKSGGHNRHWTNGRR
Sbjct: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447

BLAST of Cp4.1LG08g13540 vs. NCBI nr
Match: XP_022971199.1 (probable mediator of RNA polymerase II transcription subunit 26b [Cucurbita maxima])

HSP 1 Score: 836 bits (2159), Expect = 1.35e-304
Identity = 441/453 (97.35%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           MSAKS SLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MSAKSGSLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120
           LGCDRVELAVAGDVD ETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS
Sbjct: 61  LGCDRVELAVAGDVDCETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120

Query: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180
           FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE
Sbjct: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180

Query: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240
           IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVD+WVKATAAVHGSEVTPDSKSMPASEQEE
Sbjct: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDLWVKATAAVHGSEVTPDSKSMPASEQEE 240

Query: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300
           DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVK+ DNGRKTSNGS
Sbjct: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKERDNGRKTSNGS 300

Query: 301 QNPMRCKQQTTREVNVVAKDN------KSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQK 360
           QNPMRCKQQTTREVNVVAKDN      KSQMKEQVVKPNKPPSNANSGLSRPQKPS+VQK
Sbjct: 301 QNPMRCKQQTTREVNVVAKDNRESKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSTVQK 360

Query: 361 DNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQ 420
           DNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQ
Sbjct: 361 DNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQ 420

Query: 421 VMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           VMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR
Sbjct: 421 VMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 453

BLAST of Cp4.1LG08g13540 vs. NCBI nr
Match: XP_008443218.1 (PREDICTED: probable mediator of RNA polymerase II transcription subunit 26b [Cucumis melo] >KAA0053912.1 putative mediator of RNA polymerase II transcription subunit 26b [Cucumis melo var. makuwa] >TYK25493.1 putative mediator of RNA polymerase II transcription subunit 26b [Cucumis melo var. makuwa])

HSP 1 Score: 719 bits (1856), Expect = 1.84e-258
Identity = 386/452 (85.40%), Postives = 413/452 (91.37%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           M+AKS SLD WR+YFRNANSDIF +IDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MAAKSGSLDSWRNYFRNANSDIFGIIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDG---ETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVS 120
           LGCDRVELAVAGD+DG   ETGFKSD AGDG  FE GGSKESKVYSSRDD GEMN N+VS
Sbjct: 61  LGCDRVELAVAGDIDGDDGETGFKSDFAGDGCEFEAGGSKESKVYSSRDDIGEMNCNRVS 120

Query: 121 NYSFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQ 180
           N+SFGEAEALTDEIEQES+IVGEVLRIKEILNNFE+ESDSVLFESLRRLELMALSVD LQ
Sbjct: 121 NFSFGEAEALTDEIEQESQIVGEVLRIKEILNNFEDESDSVLFESLRRLELMALSVDTLQ 180

Query: 181 ATEIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASE 240
           ATEIGKAVN LRKHGSKRIRHL R LI EWK+MVD+WV+ATAAV G+EVTPDSKS  A+ 
Sbjct: 181 ATEIGKAVNCLRKHGSKRIRHLARVLIMEWKDMVDVWVQATAAVQGAEVTPDSKSKSAAV 240

Query: 241 QEEDDYED-GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKT 300
           +EEDDYE+ GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSG+FVK  +N RKT
Sbjct: 241 KEEDDYEEEGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGDFVKNRNNVRKT 300

Query: 301 SNGSQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPN-KPPSNANSGLSRPQKPSSVQKD 360
           SNG+QNP RC QQTTREVNV++K+NK+QMKEQVVKPN KP SN NSGL R QK S+ QKD
Sbjct: 301 SNGNQNPTRCNQQTTREVNVLSKENKTQMKEQVVKPNNKPSSNTNSGLLRQQKLSTEQKD 360

Query: 361 NHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420
           NHEK QRK+EKPTIPKR+ QD FKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV
Sbjct: 361 NHEKFQRKLEKPTIPKRNQQDNFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420

Query: 421 MELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           MELHD+PKQG+GHRN HLKSGG NRHWTNGRR
Sbjct: 421 MELHDLPKQGIGHRNPHLKSGGFNRHWTNGRR 452

BLAST of Cp4.1LG08g13540 vs. ExPASy TrEMBL
Match: A0A6J1FCK1 (probable mediator of RNA polymerase II transcription subunit 26b OS=Cucurbita moschata OX=3662 GN=LOC111442859 PE=4 SV=1)

HSP 1 Score: 840 bits (2169), Expect = 1.55e-306
Identity = 440/447 (98.43%), Postives = 442/447 (98.88%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           MSAKS SLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MSAKSGSLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120
           LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS
Sbjct: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120

Query: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180
           FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE
Sbjct: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180

Query: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240
           IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMP SEQEE
Sbjct: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPVSEQEE 240

Query: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300
           DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKK DNGRKTS+GS
Sbjct: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKRDNGRKTSSGS 300

Query: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNHEKL 360
           QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSG SR QKPS+VQKDNHEKL
Sbjct: 301 QNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNANSGFSRLQKPSTVQKDNHEKL 360

Query: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420
           QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD
Sbjct: 361 QRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHD 420

Query: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           IPKQGMGHRNVHLKSGGHNRHWTNGRR
Sbjct: 421 IPKQGMGHRNVHLKSGGHNRHWTNGRR 447

BLAST of Cp4.1LG08g13540 vs. ExPASy TrEMBL
Match: A0A6J1I7W1 (probable mediator of RNA polymerase II transcription subunit 26b OS=Cucurbita maxima OX=3661 GN=LOC111469992 PE=4 SV=1)

HSP 1 Score: 836 bits (2159), Expect = 6.52e-305
Identity = 441/453 (97.35%), Postives = 444/453 (98.01%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           MSAKS SLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MSAKSGSLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120
           LGCDRVELAVAGDVD ETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS
Sbjct: 61  LGCDRVELAVAGDVDCETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYS 120

Query: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180
           FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE
Sbjct: 121 FGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATE 180

Query: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEE 240
           IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVD+WVKATAAVHGSEVTPDSKSMPASEQEE
Sbjct: 181 IGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDLWVKATAAVHGSEVTPDSKSMPASEQEE 240

Query: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGS 300
           DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVK+ DNGRKTSNGS
Sbjct: 241 DDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKERDNGRKTSNGS 300

Query: 301 QNPMRCKQQTTREVNVVAKDN------KSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQK 360
           QNPMRCKQQTTREVNVVAKDN      KSQMKEQVVKPNKPPSNANSGLSRPQKPS+VQK
Sbjct: 301 QNPMRCKQQTTREVNVVAKDNRESKDNKSQMKEQVVKPNKPPSNANSGLSRPQKPSTVQK 360

Query: 361 DNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQ 420
           DNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQ
Sbjct: 361 DNHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQ 420

Query: 421 VMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           VMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR
Sbjct: 421 VMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 453

BLAST of Cp4.1LG08g13540 vs. ExPASy TrEMBL
Match: A0A5A7UDQ2 (Putative mediator of RNA polymerase II transcription subunit 26b OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006410 PE=4 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 8.92e-259
Identity = 386/452 (85.40%), Postives = 413/452 (91.37%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           M+AKS SLD WR+YFRNANSDIF +IDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MAAKSGSLDSWRNYFRNANSDIFGIIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDG---ETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVS 120
           LGCDRVELAVAGD+DG   ETGFKSD AGDG  FE GGSKESKVYSSRDD GEMN N+VS
Sbjct: 61  LGCDRVELAVAGDIDGDDGETGFKSDFAGDGCEFEAGGSKESKVYSSRDDIGEMNCNRVS 120

Query: 121 NYSFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQ 180
           N+SFGEAEALTDEIEQES+IVGEVLRIKEILNNFE+ESDSVLFESLRRLELMALSVD LQ
Sbjct: 121 NFSFGEAEALTDEIEQESQIVGEVLRIKEILNNFEDESDSVLFESLRRLELMALSVDTLQ 180

Query: 181 ATEIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASE 240
           ATEIGKAVN LRKHGSKRIRHL R LI EWK+MVD+WV+ATAAV G+EVTPDSKS  A+ 
Sbjct: 181 ATEIGKAVNCLRKHGSKRIRHLARVLIMEWKDMVDVWVQATAAVQGAEVTPDSKSKSAAV 240

Query: 241 QEEDDYED-GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKT 300
           +EEDDYE+ GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSG+FVK  +N RKT
Sbjct: 241 KEEDDYEEEGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGDFVKNRNNVRKT 300

Query: 301 SNGSQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPN-KPPSNANSGLSRPQKPSSVQKD 360
           SNG+QNP RC QQTTREVNV++K+NK+QMKEQVVKPN KP SN NSGL R QK S+ QKD
Sbjct: 301 SNGNQNPTRCNQQTTREVNVLSKENKTQMKEQVVKPNNKPSSNTNSGLLRQQKLSTEQKD 360

Query: 361 NHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420
           NHEK QRK+EKPTIPKR+ QD FKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV
Sbjct: 361 NHEKFQRKLEKPTIPKRNQQDNFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420

Query: 421 MELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           MELHD+PKQG+GHRN HLKSGG NRHWTNGRR
Sbjct: 421 MELHDLPKQGIGHRNPHLKSGGFNRHWTNGRR 452

BLAST of Cp4.1LG08g13540 vs. ExPASy TrEMBL
Match: A0A1S3B7K4 (probable mediator of RNA polymerase II transcription subunit 26b OS=Cucumis melo OX=3656 GN=LOC103486863 PE=4 SV=1)

HSP 1 Score: 719 bits (1856), Expect = 8.92e-259
Identity = 386/452 (85.40%), Postives = 413/452 (91.37%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           M+AKS SLD WR+YFRNANSDIF +IDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MAAKSGSLDSWRNYFRNANSDIFGIIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDG---ETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVS 120
           LGCDRVELAVAGD+DG   ETGFKSD AGDG  FE GGSKESKVYSSRDD GEMN N+VS
Sbjct: 61  LGCDRVELAVAGDIDGDDGETGFKSDFAGDGCEFEAGGSKESKVYSSRDDIGEMNCNRVS 120

Query: 121 NYSFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQ 180
           N+SFGEAEALTDEIEQES+IVGEVLRIKEILNNFE+ESDSVLFESLRRLELMALSVD LQ
Sbjct: 121 NFSFGEAEALTDEIEQESQIVGEVLRIKEILNNFEDESDSVLFESLRRLELMALSVDTLQ 180

Query: 181 ATEIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASE 240
           ATEIGKAVN LRKHGSKRIRHL R LI EWK+MVD+WV+ATAAV G+EVTPDSKS  A+ 
Sbjct: 181 ATEIGKAVNCLRKHGSKRIRHLARVLIMEWKDMVDVWVQATAAVQGAEVTPDSKSKSAAV 240

Query: 241 QEEDDYED-GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKT 300
           +EEDDYE+ GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSG+FVK  +N RKT
Sbjct: 241 KEEDDYEEEGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGDFVKNRNNVRKT 300

Query: 301 SNGSQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPN-KPPSNANSGLSRPQKPSSVQKD 360
           SNG+QNP RC QQTTREVNV++K+NK+QMKEQVVKPN KP SN NSGL R QK S+ QKD
Sbjct: 301 SNGNQNPTRCNQQTTREVNVLSKENKTQMKEQVVKPNNKPSSNTNSGLLRQQKLSTEQKD 360

Query: 361 NHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420
           NHEK QRK+EKPTIPKR+ QD FKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV
Sbjct: 361 NHEKFQRKLEKPTIPKRNQQDNFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420

Query: 421 MELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           MELHD+PKQG+GHRN HLKSGG NRHWTNGRR
Sbjct: 421 MELHDLPKQGIGHRNPHLKSGGFNRHWTNGRR 452

BLAST of Cp4.1LG08g13540 vs. ExPASy TrEMBL
Match: A0A0A0LBK9 (TFIIS N-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G817210 PE=4 SV=1)

HSP 1 Score: 708 bits (1828), Expect = 1.63e-254
Identity = 381/452 (84.29%), Postives = 409/452 (90.49%), Query Frame = 0

Query: 1   MSAKSASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60
           M+AKS SLD WR+YFRNANSDIF +IDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC
Sbjct: 1   MAAKSGSLDSWRNYFRNANSDIFGIIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRC 60

Query: 61  LGCDRVELAVAGDVDG---ETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVS 120
           LGCDRVELAVAGD+DG   ETGFKSD   DG  FE GGSKESKVYSSRDD GEMN N+VS
Sbjct: 61  LGCDRVELAVAGDIDGDDGETGFKSDFVRDGCEFEAGGSKESKVYSSRDDIGEMNCNRVS 120

Query: 121 NYSFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQ 180
           N+SFGEAEALTDEIEQES+IVGEVLRIKEILNNFE+ESD VLFESLRRLELMALSVD LQ
Sbjct: 121 NFSFGEAEALTDEIEQESQIVGEVLRIKEILNNFEDESDPVLFESLRRLELMALSVDTLQ 180

Query: 181 ATEIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASE 240
           ATEIGKAVN LRKHGSKRIRHL R LI EWKEMVD+WV+ATAAV G+EVTPDSK+  A+ 
Sbjct: 181 ATEIGKAVNCLRKHGSKRIRHLARVLIMEWKEMVDLWVQATAAVQGAEVTPDSKNKSAAV 240

Query: 241 QEEDDYED-GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKT 300
           +EEDDYE+ GLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSG+FVK  +N RKT
Sbjct: 241 KEEDDYEEEGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGDFVKNRNNVRKT 300

Query: 301 SNGSQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPN-KPPSNANSGLSRPQKPSSVQKD 360
           SNG+QNP RC QQTTREVNV++K+NK+QMKEQVVKPN KP SN NSGL R QK S+ QKD
Sbjct: 301 SNGNQNPTRCNQQTTREVNVLSKENKTQMKEQVVKPNNKPSSNTNSGLLRAQKLSTEQKD 360

Query: 361 NHEKLQRKVEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420
           NHEK QRK+EKPTIPKR+ QD FKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV
Sbjct: 361 NHEKFQRKLEKPTIPKRNQQDNFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQV 420

Query: 421 MELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 447
           MELHD+PKQG+GHRN HLKSGG NR+W NGRR
Sbjct: 421 MELHDLPKQGIGHRNPHLKSGGFNRNWANGRR 452

BLAST of Cp4.1LG08g13540 vs. TAIR 10
Match: AT5G05140.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 319.3 bits (817), Expect = 4.9e-87
Identity = 214/462 (46.32%), Postives = 280/462 (60.61%), Query Frame = 0

Query: 5   SASLDYWRDYF-RNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRCLGC 64
           S SLD WR+YF R  +SDIF +ID+AI+VAA+DCP +F+ RRD+IAE LFSCR+ RC+GC
Sbjct: 4   SGSLDSWREYFRRRGDSDIFGIIDHAIMVAATDCPNKFKSRRDKIAELLFSCRVNRCVGC 63

Query: 65  DRVELAVAGDVDGETGFKSDSAGDGRAFE----GGGSKESKVYSSRDDGGEMNFNQVSNY 124
           D +EL+V GD +   G   +  G     E     GGSKESK  SSR D  ++    VSNY
Sbjct: 64  DHLELSVPGDDEANRGTTGNGGGGTAVDEDYEVAGGSKESKANSSRGDNNQI----VSNY 123

Query: 125 SFGEAEALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQAT 184
           +F EAEAL+DEIE+ S +  EV RIKEIL N E+E +SVL +SLR L+LM+L+VD L++T
Sbjct: 124 TFDEAEALSDEIEEFSVVSKEVARIKEILLNKEDEPNSVLLDSLRHLKLMSLNVDILKST 183

Query: 185 EIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQE 244
           EIGKAVNGLRKH S +IR L + LI EWKE+VD WV  T  + G+E TP+S +    ++E
Sbjct: 184 EIGKAVNGLRKHSSDKIRQLAKTLIAEWKELVDQWVNTTKEITGAEGTPESANPSVLDEE 243

Query: 245 EDDYEDGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNG 304
           E       PS P D    FT +P   E+S FFD +D DGNPRNS E    H+  R+    
Sbjct: 244 E-----AFPSLPYD-VDIFTPEPNGFEISHFFDSLDFDGNPRNSEE----HNTSREHERR 303

Query: 305 SQNPMRCKQQTTREVNVVAKDNKSQMKEQVVKPNKPPSNAN-SGLSRPQKPSSVQKDNHE 364
            QN  + K + T          + ++++   +  KP S  +  G  RP K S+ Q+  +E
Sbjct: 304 PQNIAKRKPEGT----------QMRIQDAPFRSIKPSSATDFDGTRRPVKQSTEQRMKNE 363

Query: 365 KLQ-RKVEKPTIPKRSL------------QDKFKCSDEVAVQVKLEATKRKLQESYQQAE 424
            +   K EKP I ++ +            Q+K K  D      K E  KRKLQESYQ  E
Sbjct: 364 TVSVHKSEKPMIQRKPVVTEQKRKAPGPQQEKLKGLD---ADAKFEFAKRKLQESYQHHE 423

Query: 425 NAKKQRTIQVMELHDIPKQGMGHRNVHLKSGGHNRHWTNGRR 448
           NAKKQRTIQV+E+  IPKQG   +    + G  NR+W NGR+
Sbjct: 424 NAKKQRTIQVLEM--IPKQGSAQKPQLKRPGMSNRNWANGRK 436

BLAST of Cp4.1LG08g13540 vs. TAIR 10
Match: AT3G10820.2 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 253.8 bits (647), Expect = 2.5e-67
Identity = 178/428 (41.59%), Postives = 249/428 (58.18%), Query Frame = 0

Query: 5   SASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRCLGCD 64
           S SLD WR+YFR  +SDIF +ID+AI+VAA+D PKEF+ R DRIAE LFSC+++RC+GCD
Sbjct: 7   SVSLDTWREYFRRGDSDIFGIIDHAIMVAAADWPKEFKSRSDRIAELLFSCKVSRCIGCD 66

Query: 65  RVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYSFGEA 124
            +EL++AGD         ++A +     GGG +                        G++
Sbjct: 67  HLELSIAGD---------EAAVEIVGVGGGGDR------------------------GDS 126

Query: 125 EALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATEIGKA 184
              T E E+ S  V EV+RI++IL+N ++E DSVL ESLR+LE M++SVD L+ TEIGKA
Sbjct: 127 GVATGEGEEASVSVDEVMRIRDILSNKDDEKDSVLLESLRKLESMSMSVDILKDTEIGKA 186

Query: 185 VNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEEDDYE 244
           VNGLR+H S +I  L + L  EWK +VD W+     + G+E TP+S ++   ++EE    
Sbjct: 187 VNGLRRHSSDKISKLAKTLFAEWKRLVDQWMNTPEEMAGTEGTPESLNLSVIDEEE---- 246

Query: 245 DGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGSQNPM 304
              PSPP D    +  +P   ELSQ  D +D DGNPR+S E   KH+   ++S G     
Sbjct: 247 -AFPSPPHD-LDIYAPEPNGFELSQILDCLDCDGNPRHSVE--SKHERKSQSSAGR---- 306

Query: 305 RCKQQTTREVNVVAK---DNKSQMKEQVVKPNKPPSNANSGLSRPQKPSSVQKDNH-EKL 364
             + + T + NVV +   D +++ +E  V+P K   ++ + +  P++ +   ++     +
Sbjct: 307 --RPKGTNDANVVGRYCNDQQTRREEADVRPMK---HSATDVVEPKRQTKQSREQMVSAI 366

Query: 365 QRKVEKPTIPKRSL----QDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVM 424
           QRK    T  KR L    QDK K  D      K E  KRKLQESY Q ENAK+QRTIQV+
Sbjct: 367 QRKPTAVTEQKRKLAGPQQDKLKALDP---DSKFEFAKRKLQESYHQHENAKRQRTIQVL 379

BLAST of Cp4.1LG08g13540 vs. TAIR 10
Match: AT3G10820.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 202.6 bits (514), Expect = 6.6e-52
Identity = 117/265 (44.15%), Postives = 162/265 (61.13%), Query Frame = 0

Query: 5   SASLDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRLTRCLGCD 64
           S SLD WR+YFR  +SDIF +ID+AI+VAA+D PKEF+ R DRIAE LFSC+++RC+GCD
Sbjct: 7   SVSLDTWREYFRRGDSDIFGIIDHAIMVAAADWPKEFKSRSDRIAELLFSCKVSRCIGCD 66

Query: 65  RVELAVAGDVDGETGFKSDSAGDGRAFEGGGSKESKVYSSRDDGGEMNFNQVSNYSFGEA 124
            +EL++AGD         ++A +     GGG +                        G++
Sbjct: 67  HLELSIAGD---------EAAVEIVGVGGGGDR------------------------GDS 126

Query: 125 EALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATEIGKA 184
              T E E+ S  V EV+RI++IL+N ++E DSVL ESLR+LE M++SVD L+ TEIGKA
Sbjct: 127 GVATGEGEEASVSVDEVMRIRDILSNKDDEKDSVLLESLRKLESMSMSVDILKDTEIGKA 186

Query: 185 VNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEEDDYE 244
           VNGLR+H S +I  L + L  EWK +VD W+     + G+E TP+S ++   ++EE    
Sbjct: 187 VNGLRRHSSDKISKLAKTLFAEWKRLVDQWMNTPEEMAGTEGTPESLNLSVIDEEE---- 232

Query: 245 DGLPSPPLDEAAFFTTQPTSMELSQ 270
              PSPP D    +  +P   ELSQ
Sbjct: 247 -AFPSPPHD-LDIYAPEPNGFELSQ 232

BLAST of Cp4.1LG08g13540 vs. TAIR 10
Match: AT5G09850.1 (Transcription elongation factor (TFIIS) family protein )

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-21
Identity = 124/442 (28.05%), Postives = 194/442 (43.89%), Query Frame = 0

Query: 8   LDYWRDYFRNANSDIFSLIDYAILVAASDCPKEFRLRRDRIAEQLFSCRL-TRCLGCDRV 67
           LD +R    NA  D+++ ID AILVA+ D  +E + RRD I E+L++  +  +C  CD  
Sbjct: 3   LDDFRSVMDNAGVDVWTFIDTAILVASLDYGQELKRRRDNIVERLYATSMANKCRNCD-- 62

Query: 68  ELAVAGDVDGETGFKSDSAGDGRAFE--GGGSKESKVYSSRDDGGEMNFNQVSNYSFGEA 127
                G     T        +GR  E      +E    ++ ++  E + N   +  F   
Sbjct: 63  ----FGGGGNVTEAAIGRVNNGRVHEETEEEDEEGVTAAAEEEVREKSVNVEDDDDFDPF 122

Query: 128 EALTDEIEQESEIVGEVLRIKEILNNFEEESDSVLFESLRRLELMALSVDALQATEIGKA 187
             L D+ EQ+S     ++ IKE L +  + S+  L E L+ LE M ++  ALQ T+IG+ 
Sbjct: 123 AGLFDD-EQKS-----IVEIKEKLED-PDLSEESLVELLQNLEDMDITFQALQETDIGRH 182

Query: 188 VNGLRKHGSKRIRHLVRFLITEWKEMVDMWVKATAAVHGSEVTPDSKSMPASEQEEDDYE 247
           VN +RKH S  +R L + L+ +WKE VD WVK           P     P+   +ED   
Sbjct: 183 VNRVRKHPSNNVRRLAKQLVKKWKETVDEWVKFN--------QPGDLEPPSLIADEDS-- 242

Query: 248 DGLPSPPLDEAAFFTTQPTSMELSQFFDGMDDDGNPRNSGEFVKKHDNGRKTSNGSQNPM 307
                 P+ +A                     +G+ +   +F             S  P 
Sbjct: 243 ------PVQKAL-------------------HNGSRQQVPDF-----------GYSPVPQ 302

Query: 308 RCKQQTTREVNVVAKDNKSQMKEQVVKPNKP-PSNANSGLSRPQKPSSVQKDNHEKLQRK 367
                +++  N+   + K        +P  P P   +   ++P +PS  Q+         
Sbjct: 303 NGYSSSSKNSNITEPERKP-------RPVAPQPRRESPSPAKPSRPSPSQQ--------- 353

Query: 368 VEKPTIPKRSLQDKFKCSDEVAVQVKLEATKRKLQESYQQAENAKKQRTIQVMELHDIPK 427
               TIP+          D+   +V  +  +++LQ++Y+QAENAKKQRTIQVM++HDIPK
Sbjct: 363 ----TIPR----------DKEHKEVDFDTARKRLQQNYRQAENAKKQRTIQVMDIHDIPK 353

Query: 428 QGMGHRNVHLKSGGHN---RHW 443
              G      + GG +   RHW
Sbjct: 423 PKKG--GFFPRKGGSSQGGRHW 353

BLAST of Cp4.1LG08g13540 vs. TAIR 10
Match: AT3G48060.1 (BAH domain ;TFIIS helical bundle-like domain )

HSP 1 Score: 43.9 bits (102), Expect = 3.9e-04
Identity = 22/57 (38.60%), Postives = 33/57 (57.89%), Query Frame = 0

Query: 156 DSVLFESLRRLELMALSVDALQATEIGKAVNGLRKHGSKRIRHLVRFLITEWKEMVD 213
           D  L   LR L+ + ++++ALQ   IGK+VN LR H +  I    R L+  WK+ V+
Sbjct: 354 DDFLLVLLRALDKLPVNLNALQTCNIGKSVNHLRSHKNSEIGKKARSLVDTWKKRVE 410

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FHK96.9e-8646.32Probable mediator of RNA polymerase II transcription subunit 26b OS=Arabidopsis ... [more]
F4J4Y53.5e-6641.59Probable mediator of RNA polymerase II transcription subunit 26a OS=Arabidopsis ... [more]
F4KFC71.7e-2028.05Probable mediator of RNA polymerase II transcription subunit 26c OS=Arabidopsis ... [more]
Match NameE-valueIdentityDescription
XP_023539160.15.19e-312100.00probable mediator of RNA polymerase II transcription subunit 26b [Cucurbita pepo... [more]
KAG6596180.12.26e-30698.43putative mediator of RNA polymerase II transcription subunit 26b, partial [Cucur... [more]
XP_022936188.13.21e-30698.43probable mediator of RNA polymerase II transcription subunit 26b [Cucurbita mosc... [more]
XP_022971199.11.35e-30497.35probable mediator of RNA polymerase II transcription subunit 26b [Cucurbita maxi... [more]
XP_008443218.11.84e-25885.40PREDICTED: probable mediator of RNA polymerase II transcription subunit 26b [Cuc... [more]
Match NameE-valueIdentityDescription
A0A6J1FCK11.55e-30698.43probable mediator of RNA polymerase II transcription subunit 26b OS=Cucurbita mo... [more]
A0A6J1I7W16.52e-30597.35probable mediator of RNA polymerase II transcription subunit 26b OS=Cucurbita ma... [more]
A0A5A7UDQ28.92e-25985.40Putative mediator of RNA polymerase II transcription subunit 26b OS=Cucumis melo... [more]
A0A1S3B7K48.92e-25985.40probable mediator of RNA polymerase II transcription subunit 26b OS=Cucumis melo... [more]
A0A0A0LBK91.63e-25484.29TFIIS N-terminal domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G8... [more]
Match NameE-valueIdentityDescription
AT5G05140.14.9e-8746.32Transcription elongation factor (TFIIS) family protein [more]
AT3G10820.22.5e-6741.59Transcription elongation factor (TFIIS) family protein [more]
AT3G10820.16.6e-5244.15Transcription elongation factor (TFIIS) family protein [more]
AT5G09850.11.2e-2128.05Transcription elongation factor (TFIIS) family protein [more]
AT3G48060.13.9e-0438.60BAH domain ;TFIIS helical bundle-like domain [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 387..414
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..255
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 351..378
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 433..447
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 269..378
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 296..350
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 426..447
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 85..104
NoneNo IPR availablePANTHERPTHR46554:SF2MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26A-RELATEDcoord: 1..446
NoneNo IPR availableCDDcd00183TFIIS_Icoord: 140..212
e-value: 3.2049E-20
score: 82.3562
IPR003617Transcription elongation factor, TFIIS/CRSP70, N-terminal, sub-typeSMARTSM00509TFS2_5coord: 141..213
e-value: 6.9E-15
score: 65.4
IPR035441TFIIS/LEDGF domain superfamilyGENE3D1.20.930.10Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 130..223
e-value: 1.0E-20
score: 75.3
IPR035441TFIIS/LEDGF domain superfamilySUPERFAMILY47676Conserved domain common to transcription factors TFIIS, elongin A, CRSP70coord: 137..222
IPR017923Transcription factor IIS, N-terminalPFAMPF08711Med26coord: 163..211
e-value: 5.3E-10
score: 39.2
IPR017923Transcription factor IIS, N-terminalPROSITEPS51319TFIIS_Ncoord: 139..214
score: 20.201963
IPR044805Mediator of RNA polymerase II transcription subunit 26a/b-likePANTHERPTHR46554MEDIATOR OF RNA POLYMERASE II TRANSCRIPTION SUBUNIT 26A-RELATEDcoord: 1..446

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g13540.1Cp4.1LG08g13540.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus