Cp4.1LG08g12220 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g12220
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionS-adenosyl-L-methionine-dependent methyltransferases superfamily protein
LocationCp4.1LG08: 8865966 .. 8867804 (-)
RNA-Seq ExpressionCp4.1LG08g12220
SyntenyCp4.1LG08g12220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTTCTTGTATTGGGTCATCTCTCTTAAAATATTTTTACCTTTCTGCTAGGATGTCAATCACTGAGATGGTCCAGATGAATGGTAATTGGTACTAAATATTCCTTTCCAAGAATACTAATTTCCTTTGTTTGTTGGGAGTTCTATGTCCTGGAGATGTCATTATTTCCTCGCGAACAATGCCGCAATATTTTGGATTAACCTTCCATAACAGAATAGTTTTCCAGAATCCTCTAGAAATCTGTGTGATCTATTTAGTTTTGCAATCAACCTGTGTTATCTTGTTCATGAACTTTCAATTGCAGGAGTCAAGCGAGTGGTGAATTTATTCGCAGCCCCTGGTAGCTGGAGCTAGGTAATCTCTTTTGGGTGTCTTCTTGTTGCAATCTGTTTATCCAACTAGTGGACAATGCTTTATGGGGGAAATACAATTTTTCATATAAAATACAACTAATTCTTACAAGAATTCTGCAATATGAATCTCTCTTCTCATCTAGTTTCCTTTTTTCTTAGTCAGTCAAGTTGAAAGGTTTGCTGTGATCTCCTCTGTTCCTTGAGATTATTGCTTTGACGTGTAGATTATTTTTGACATGTTTAGCATTGGTAAAACATGTCAAGTATTATAATGAATTACGTGAGGACCATAATCAAAGCTAGGTTTCTGTTGTTTTACATTTTCGAGTTTTCATTTTTGTTGCAGCTGAAGTTGCTTTTCCCGTAGTGACTTTTGCAAAACCGAAAGGCAGTCGCAATTCCAGTATAGGTAACCTTTTGGCTTCACTTTTTTTATTGGTAACTCTATAGGTCTATCTTTCGGTTATAATTGAACTTATTTCCTGAAAATGATACCCTTTTTCTAAGTGAAAATTGTCATTATAGCGTCTGGTTCATAGGATTCTTACGCATGGATTTCAGATCCCATTGAACTTTAGATTGTTATCGTTTAATACTAGGTGGGACTAGTGTCCACCCAACACCAAATTGTGGGGTTTGCTTATGTTCGTGGTGGCATCTGTAATATGAAAAAAAAAAACATGTAAAAGAGGACAAGATAGTCAAAAGGCAGCTCACATATTTTCTAAAACTTCGATGGTTATGTTTATGAACATTAGAAAGTTTTGAATTACAACTATGAATTGTTCTATTTGATTCTAGGTAGTCGGTCGTTTAATACCATTGCTCAAATGTTGACGTGTCAGGCTGATTAAACTATAACTAGTTACAACTTACAAGTGATTATTTGGAATCATTTAACTTTTAAACAAAGTAGTGACTATGATTTAACTTTATGTTCATGTTGAAGCTCATACGTGGACTGGGTGTGAATGTTGAGGCATTTGTAGTTTGTGAGAATTATTCGCCTCCTGAGGGTTTCAACCCATAAGATCCAACCCCTGCTAGAAAAGGTGGGAAGCCCATCAGGAGGAGATGACTTAGGTAAACTCGATGTACGACCTTGATTGGGAGTTCACATATCGAAGGACGTGATACAACATGTTCTTGTTCTTTAAAGATTAATATGTTTTACAGCTGGCTATGACCATGTCTTCTTGTCTTTAAACTTTATATAATTAGTGTCTCTGATTTCAATGTGATCAATTCCCTGGTATTTTTGGTTACACAAGAGGAAGTAATAGCATTGCTATTTTGCTGCATTGTGTCCCTCTCAGATTGCAGTAGTGGGTGGCTGGGAGGACCCAATAAAGTATCCCCTCAGCGGATATGATTCCGATCGTTCATACCTACTGCCCAGAGGTGCCGAGGGAACTTTGATCCTGGTGCAACCTCCTATTGCCCCTCCTTACAACAGGGCTCTTGAAATGAAGAAAACCTTCCAGTGA

mRNA sequence

TGTTCTTGTATTGGGTCATCTCTCTTAAAATATTTTTACCTTTCTGCTAGGATGTCAATCACTGAGATGGTCCAGATGAATGGAGTCAAGCGAGTGGTGAATTTATTCGCAGCCCCTGCTGAAGTTGCTTTTCCCGTAGTGACTTTTGCAAAACCGAAAGGCAGTCGCAATTCCAGTATAGTAGTGGGTGGCTGGGAGGACCCAATAAAGTATCCCCTCAGCGGATATGATTCCGATCGTTCATACCTACTGCCCAGAGGTGCCGAGGGAACTTTGATCCTGGTGCAACCTCCTATTGCCCCTCCTTACAACAGGGCTCTTGAAATGAAGAAAACCTTCCAGTGA

Coding sequence (CDS)

ATGTCAATCACTGAGATGGTCCAGATGAATGGAGTCAAGCGAGTGGTGAATTTATTCGCAGCCCCTGCTGAAGTTGCTTTTCCCGTAGTGACTTTTGCAAAACCGAAAGGCAGTCGCAATTCCAGTATAGTAGTGGGTGGCTGGGAGGACCCAATAAAGTATCCCCTCAGCGGATATGATTCCGATCGTTCATACCTACTGCCCAGAGGTGCCGAGGGAACTTTGATCCTGGTGCAACCTCCTATTGCCCCTCCTTACAACAGGGCTCTTGAAATGAAGAAAACCTTCCAGTGA

Protein sequence

MSITEMVQMNGVKRVVNLFAAPAEVAFPVVTFAKPKGSRNSSIVVGGWEDPIKYPLSGYDSDRSYLLPRGAEGTLILVQPPIAPPYNRALEMKKTFQ
Homology
BLAST of Cp4.1LG08g12220 vs. ExPASy Swiss-Prot
Match: O36015 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC4F10.03c PE=3 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 5.4e-04
Identity = 34/111 (30.63%), Postives = 49/111 (44.14%), Query Frame = 0

Query: 14  RVVNLFAAPAEVAFPVVTFAKPKGSRNSSI----VVGGWEDPIKY--------------- 73
           R V+L  +   + F  V+ AKP+ SR SSI    V   +  P  +               
Sbjct: 169 RDVSLLYSQLRLMFRKVSCAKPRSSRASSIESFVVCEDFNPPSNFQPDLTKPLCVIDPTN 228

Query: 74  -----------PLSGYDSDRSYLLP-RGAEGTLILVQPPIAPPYNRALEMK 94
                       L GYD+D +Y +     + TL ++QPP APPY RA+E+K
Sbjct: 229 AHEIAPFIACGDLDGYDADATYPVEINMKKATLDVIQPPTAPPYKRAIELK 279

BLAST of Cp4.1LG08g12220 vs. NCBI nr
Match: XP_008442455.1 (PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442456.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo] >XP_008442457.1 PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 78.2 bits (191), Expect = 1.28e-14
Identity = 57/127 (44.88%), Postives = 61/127 (48.03%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L  +  ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. NCBI nr
Match: XP_004137730.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_011651872.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >XP_031738324.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativus] >KGN58784.1 hypothetical protein Csa_000714 [Cucumis sativus])

HSP 1 Score: 78.2 bits (191), Expect = 1.28e-14
Identity = 57/127 (44.88%), Postives = 61/127 (48.03%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L  +  ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. NCBI nr
Match: XP_022934133.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_022934134.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita moschata] >XP_023528668.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >XP_023528669.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita pepo subsp. pepo] >KAG7017237.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 77.8 bits (190), Expect = 1.77e-14
Identity = 57/127 (44.88%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L     ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. NCBI nr
Match: XP_038903231.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa hispida])

HSP 1 Score: 77.8 bits (190), Expect = 1.77e-14
Identity = 57/127 (44.88%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L     ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRCLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. NCBI nr
Match: XP_022983316.1 (putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima] >XP_022983317.1 putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita maxima])

HSP 1 Score: 77.8 bits (190), Expect = 1.77e-14
Identity = 57/127 (44.88%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L     ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. ExPASy TrEMBL
Match: A0A1S3B6C8 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 6.18e-15
Identity = 57/127 (44.88%), Postives = 61/127 (48.03%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L  +  ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. ExPASy TrEMBL
Match: A0A0A0LCV6 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G732420 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 6.18e-15
Identity = 57/127 (44.88%), Postives = 61/127 (48.03%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L  +  ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYSQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNSKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. ExPASy TrEMBL
Match: A0A6J1IYY9 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111481931 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 8.58e-15
Identity = 57/127 (44.88%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L     ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPLEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. ExPASy TrEMBL
Match: A0A6J1F1P7 (Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111441398 PE=3 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 8.58e-15
Identity = 57/127 (44.88%), Postives = 60/127 (47.24%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L     ++ FPVVTFAKPK SRNSSI                                 
Sbjct: 174 SLLYCQLKLFFPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYN 94
                  GW E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY 
Sbjct: 234 DDLDCSSGWLEGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYK 293

BLAST of Cp4.1LG08g12220 vs. ExPASy TrEMBL
Match: A0A1S4DVJ2 (putative tRNA (Cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103486316 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 8.73e-15
Identity = 56/117 (47.86%), Postives = 56/117 (47.86%), Query Frame = 0

Query: 27  FPVVTFAKPKGSRNSSIVV-------------------------------------GGW- 86
           FPVVTFAKPK SRNSSI                                        GW 
Sbjct: 153 FPVVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPKDLHRLLEKVGSPSGGDDLDCSSGWL 212

Query: 87  EDPIKY--------PLSGYDSDRSYLLPRGAEGT---LILVQPPIAPPYNRALEMKK 94
           E P K          LSGYDSDRSY LPRGAEGT   L  VQPPIAPPY RALEMKK
Sbjct: 213 EGPNKVYIPFLACGDLSGYDSDRSYPLPRGAEGTYRSLDPVQPPIAPPYKRALEMKK 269

BLAST of Cp4.1LG08g12220 vs. TAIR 10
Match: AT5G01230.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 69.7 bits (169), Expect = 1.5e-12
Identity = 50/128 (39.06%), Postives = 58/128 (45.31%), Query Frame = 0

Query: 17  NLFAAPAEVAFPVVTFAKPKGSRNSSIVV------------------------------- 76
           +L     ++ FP VTFAKPK SRNSSI                                 
Sbjct: 174 SLLYCQLKLFFPTVTFAKPKSSRNSSIEAFAVCENYSPPEGFNPRDLHRLLEKVGSPSGG 233

Query: 77  ------GGW-EDPIKY--------PLSGYDSDRSYLLPRGAEG----TLILVQPPIAPPY 95
                  GW E P K          L+GYDSDRSY LPR A+G    +L  +QPPIAPPY
Sbjct: 234 SDLDCSSGWLEGPNKVYIPFLACGDLTGYDSDRSYPLPREADGSSYQSLDPIQPPIAPPY 293

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O360155.4e-0430.63Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Schizosacch... [more]
Match NameE-valueIdentityDescription
XP_008442455.11.28e-1444.88PREDICTED: putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase iso... [more]
XP_004137730.11.28e-1444.88putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucumis sativ... [more]
XP_022934133.11.77e-1444.88putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita mos... [more]
XP_038903231.11.77e-1444.88putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Benincasa his... [more]
XP_022983316.11.77e-1444.88putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase [Cucurbita max... [more]
Match NameE-valueIdentityDescription
A0A1S3B6C86.18e-1544.88Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis mel... [more]
A0A0A0LCV66.18e-1544.88Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucumis sat... [more]
A0A6J1IYY98.58e-1544.88Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m... [more]
A0A6J1F1P78.58e-1544.88Putative tRNA (cytidine(32)/guanosine(34)-2'-O)-methyltransferase OS=Cucurbita m... [more]
A0A1S4DVJ28.73e-1547.86putative tRNA (Cytidine(32)/guanosine(34)-2'-O)-methyltransferase isoform X2 OS=... [more]
Match NameE-valueIdentityDescription
AT5G01230.11.5e-1239.06S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR10920RIBOSOMAL RNA METHYLTRANSFERASEcoord: 56..95
NoneNo IPR availablePANTHERPTHR10920:SF25TRNA (CYTIDINE(32)/GUANOSINE(34)-2'-O)-METHYLTRANSFERASE-RELATEDcoord: 56..95

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g12220.1Cp4.1LG08g12220.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034641 cellular nitrogen compound metabolic process
biological_process GO:0043170 macromolecule metabolic process
biological_process GO:0044238 primary metabolic process