Cp4.1LG08g12030 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g12030
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCp4.1LG08: 8814222 .. 8816514 (+)
RNA-Seq ExpressionCp4.1LG08g12030
SyntenyCp4.1LG08g12030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTTTCTGGAGAGGGAGGCTATTAGTTTCATCTTGTTTTGGGTAGAGAGGTGGAGGGGGATTCTTTAAGGGAGAAATATGCTTCAAATCCGGCTTAGCAAAGTTCCACAGGCGGAAGGTGGCGCTGGAACAGTGAAGCCCTTGCAAGCTGAGACTGTGACGGTCGCATGCCCTGACCACCTTATCCTTGCTGATCTTCCTGTGGCAAATGGCATTGGTGCAGCTACTGCTGCTTCCATTGTTAAGTCTGTTGGTCGCAGATCTCGCCGTCACCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCCATTGCAGTTTATGGTCGCCTGGTATTTTCCCCACTATATTTTTGTGTCTTTTTGCTGTCGGTTGCTTTGTCTCCTTTCGGTCCATGGAAATCATTCATCTATAAAAGACAATTTAAGATCAACTTTTTTTTTTGTCAAATCGATATGCATTACTTCATTCATATCTGCAATGTTCTTCCTTCTTGCCCGCTGGTGTGTAGGCTTGTGTCATTTCTTTTCAAATATTTGTGAGCATCTCTAGCCTGTGTGCATCTTGACTAATCTCACAGGACACCCACTTGACCCTACTACATTTGGGTTGTGGCATACTTAAAGAGCTAAATAGTGGTTTGCCATTCTTGCAGAGCCCATGTGAGCATGTGTTCTGTCTCGATTGTGCAAGAAGCGATTCTATATGCTACCTGTAAGTAAAAATGCTTTGAAAGTCTTAAAATGGCTGTTATGGTTCTTTTGTACTATCAATGTCAATATTGATTGCCGATGCATAATCACTTTCTATTGCAGAAACTTTTCTTGTCATGTTCTTCAAAAATCTGTTGTTTTCTTTGATGATTAGAGTACTCTATACATTAGCTTCTGTGTTCTTCATGATAATATAATGTAGACGACTTTAGAAGTTGTGATTATAGTCATATTTTTTATGACACAGTTGTGATGACCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACATTGTCTCAAGTCTTTTCTTAAGAGGACTGAATTTGAATCTCATATACAAGAGAGTCATGCTGACCTTCTTAAGCCAAATGCAGAGAAGGAAGATGGAAACGAAATTGAGGCTCTTAGTGCCAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCATCGAGACCAATCATATCTCCAAGTTCCAATTCCCTGGCTCAAGAACGTGACGAAAAATTTCATCGTCAACAATCTAGAGACCAACCCACGTCTGGCATGCAGCAACAAGACAGCGGTGGTCATTCACAAGGCTTTGATCGACATGGTCCTCATGGCCATTTTCCCCCACAAAACTTTGACGTACAGGGTACTCCACATCAGGAATCCAGTCAATTTTCAGAGAAACAGCAGGGAATTCTGGCCGACCCTCCATATTCTCAGTACCCTCCTTTGCAATCAATTCCGCCACCGAATTACGTGGTTCCAGCTAACTCAAATCCAATGTTGAGTCCCCCTCTACCGTTCGGTTTTCCTCCGTTTCCAACTGAGGGAGCACAACAATATTACAGTACACCCTATGAAGTGCCACGACAAGATACCGCAGCAGAGACTGGATCCGAGCAAGGGTCACTGCTAGGCTTTCCACCAGGAGCAGCAGGAGGAATGAACTTCTCGGCGACTTATCCTCAACCTTGGAATACTGGACAAGCTGGCGTTCCTTATGAGCATGCTGCTGGAGGTCAAGGAATGCCTGATACTTTTGCTAATGCACCTGATTCTCAAGTAAATCATGGATTTTACCAAGGAGATTATAGACGCAGCCCTGGAGGCTTGCCTGTGAATTCTTCTGTCTCGTCAATGGCCAACAAGAGCATGGACCTGGGGCATTCTGGTAATGCCATGGACTTGAAGAATGGTAGAGGTATATTGGCACCACAGCCGCCTCTAGCACAACTTCCTCTGCCTCCGCCACTCCCACTGTACTTGTCGCATAATAAGCGTGGCAAGTTTCATTCAGGAGATACGGATCATGATGGGCAAGGCCATGGATGGCAGAATGATAGCCATAGCCGAGATAGCTTTGGAAATGGCCAAGACTGATGATTCTTCCATCGCATTCTTTGTTTCTTTTGCCATATACTTTCAACCATTATTTTGTCCAAAGTTGCATCTGCCAGCTTGAGAATGTCTATATTTAAACTTGATGATCAACGATAAAGTTTTACTTTCATTCGACAGGTTCGAGAATATTATATGCAATACTGTCGGTAGACAAGA

mRNA sequence

GGTTTCTGGAGAGGGAGGCTATTAGTTTCATCTTGTTTTGGGTAGAGAGGTGGAGGGGGATTCTTTAAGGGAGAAATATGCTTCAAATCCGGCTTAGCAAAGTTCCACAGGCGGAAGGTGGCGCTGGAACAGTGAAGCCCTTGCAAGCTGAGACTGTGACGGTCGCATGCCCTGACCACCTTATCCTTGCTGATCTTCCTGTGGCAAATGGCATTGGTGCAGCTACTGCTGCTTCCATTGTTAAGTCTGTTGGTCGCAGATCTCGCCGTCACCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCCATTGCAGTTTATGGTCGCCTGAAGCGATTCTATATGCTACCTGTAAGTAAAAATGCTTTGAAAGTCTTAAAATGGCTGTTATGTTGTGATGACCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACATTGTCTCAAGTCTTTTCTTAAGAGGACTGAATTTGAATCTCATATACAAGAGAGTCATGCTGACCTTCTTAAGCCAAATGCAGAGAAGGAAGATGGAAACGAAATTGAGGCTCTTAGTGCCAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCATCGAGACCAATCATATCTCCAAGTTCCAATTCCCTGGCTCAAGAACGTGACGAAAAATTTCATCGTCAACAATCTAGAGACCAACCCACGTCTGGCATGCAGCAACAAGACAGCGGTGGTCATTCACAAGGCTTTGATCGACATGGTCCTCATGGCCATTTTCCCCCACAAAACTTTGACGTACAGGGTACTCCACATCAGGAATCCAGTCAATTTTCAGAGAAACAGCAGGGAATTCTGGCCGACCCTCCATATTCTCAGTACCCTCCTTTGCAATCAATTCCGCCACCGAATTACGTGGTTCCAGCTAACTCAAATCCAATGTTGAGTCCCCCTCTACCGTTCGGTTTTCCTCCGTTTCCAACTGAGGGAGCACAACAATATTACAGTACACCCTATGAAGTGCCACGACAAGATACCGCAGCAGAGACTGGATCCGAGCAAGGGTCACTGCTAGGCTTTCCACCAGGAGCAGCAGGAGGAATGAACTTCTCGGCGACTTATCCTCAACCTTGGAATACTGGACAAGCTGGCGTTCCTTATGAGCATGCTGCTGGAGGTCAAGGAATGCCTGATACTTTTGCTAATGCACCTGATTCTCAAGTAAATCATGGATTTTACCAAGGAGATTATAGACGCAGCCCTGGAGGCTTGCCTGTGAATTCTTCTGTCTCGTCAATGGCCAACAAGAGCATGGACCTGGGGCATTCTGGTAATGCCATGGACTTGAAGAATGGTAGAGGTATATTGGCACCACAGCCGCCTCTAGCACAACTTCCTCTGCCTCCGCCACTCCCACTGTACTTGTCGCATAATAAGCGTGGCAAGTTTCATTCAGGAGATACGGATCATGATGGGCAAGGCCATGGATGGCAGAATGATAGCCATAGCCGAGATAGCTTTGGAAATGGCCAAGACTGATGATTCTTCCATCGCATTCTTTGTTTCTTTTGCCATATACTTTCAACCATTATTTTGTCCAAAGTTGCATCTGCCAGCTTGAGAATGTCTATATTTAAACTTGATGATCAACGATAAAGTTTTACTTTCATTCGACAGGTTCGAGAATATTATATGCAATACTGTCGGTAGACAAGA

Coding sequence (CDS)

ATGCTTCAAATCCGGCTTAGCAAAGTTCCACAGGCGGAAGGTGGCGCTGGAACAGTGAAGCCCTTGCAAGCTGAGACTGTGACGGTCGCATGCCCTGACCACCTTATCCTTGCTGATCTTCCTGTGGCAAATGGCATTGGTGCAGCTACTGCTGCTTCCATTGTTAAGTCTGTTGGTCGCAGATCTCGCCGTCACCTTGGAGAACGAGTTCATTTTTGTGTCAGATGCGATTTTCCCATTGCAGTTTATGGTCGCCTGAAGCGATTCTATATGCTACCTGTAAGTAAAAATGCTTTGAAAGTCTTAAAATGGCTGTTATGTTGTGATGACCGTATCCAGAAGATTCAAACTATAAAATTGATGGAGGGGATCTTCATTTGTGCAGCCCCACATTGTCTCAAGTCTTTTCTTAAGAGGACTGAATTTGAATCTCATATACAAGAGAGTCATGCTGACCTTCTTAAGCCAAATGCAGAGAAGGAAGATGGAAACGAAATTGAGGCTCTTAGTGCCAAACAATCTACAGCTTCTGAATCCACTGTCCGAGGTCCATCGAGACCAATCATATCTCCAAGTTCCAATTCCCTGGCTCAAGAACGTGACGAAAAATTTCATCGTCAACAATCTAGAGACCAACCCACGTCTGGCATGCAGCAACAAGACAGCGGTGGTCATTCACAAGGCTTTGATCGACATGGTCCTCATGGCCATTTTCCCCCACAAAACTTTGACGTACAGGGTACTCCACATCAGGAATCCAGTCAATTTTCAGAGAAACAGCAGGGAATTCTGGCCGACCCTCCATATTCTCAGTACCCTCCTTTGCAATCAATTCCGCCACCGAATTACGTGGTTCCAGCTAACTCAAATCCAATGTTGAGTCCCCCTCTACCGTTCGGTTTTCCTCCGTTTCCAACTGAGGGAGCACAACAATATTACAGTACACCCTATGAAGTGCCACGACAAGATACCGCAGCAGAGACTGGATCCGAGCAAGGGTCACTGCTAGGCTTTCCACCAGGAGCAGCAGGAGGAATGAACTTCTCGGCGACTTATCCTCAACCTTGGAATACTGGACAAGCTGGCGTTCCTTATGAGCATGCTGCTGGAGGTCAAGGAATGCCTGATACTTTTGCTAATGCACCTGATTCTCAAGTAAATCATGGATTTTACCAAGGAGATTATAGACGCAGCCCTGGAGGCTTGCCTGTGAATTCTTCTGTCTCGTCAATGGCCAACAAGAGCATGGACCTGGGGCATTCTGGTAATGCCATGGACTTGAAGAATGGTAGAGGTATATTGGCACCACAGCCGCCTCTAGCACAACTTCCTCTGCCTCCGCCACTCCCACTGTACTTGTCGCATAATAAGCGTGGCAAGTTTCATTCAGGAGATACGGATCATGATGGGCAAGGCCATGGATGGCAGAATGATAGCCATAGCCGAGATAGCTTTGGAAATGGCCAAGACTGA

Protein sequence

MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGRRSRRHLGERVHFCVRCDFPIAVYGRLKRFYMLPVSKNALKVLKWLLCCDDRIQKIQTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGWQNDSHSRDSFGNGQD
Homology
BLAST of Cp4.1LG08g12030 vs. ExPASy Swiss-Prot
Match: Q9LFC0 (E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAKAI PE=1 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.5e-65
Identity = 172/372 (46.24%), Postives = 222/372 (59.68%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRL +    E G G  +P   ETVTVACPDHL+LADLPVA GIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNG-ARPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL----KRFYMLPVSKNALKVLKWLLCCDDRIQKIQ 120
           RSRR LGERVHFCVRCDFPIA+YGRL      F +     +++  L     CD+RIQKIQ
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYL-----CDERIQKIQ 120

Query: 121 TIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSA-KQST 180
           TIK+MEGIFICAAPHCL+SFLK+ +FE+H+ + H  LL+ +AEKEDGN+ +  S  +QS+
Sbjct: 121 TIKMMEGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSS 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASEST+R P R  +                 QQSR+   S    +   G SQ        
Sbjct: 181 ASESTLRAPLRSQL-----------------QQSRELNRSASFAKSQSGFSQ-------V 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
            ++PP + D    P  E+   +  + GI   P Y Q  P+  + PP+  VP N NP L  
Sbjct: 241 QNYPPDS-DNSRPPGFET---ASPKPGI-RFPDYPQ--PMNLMQPPSLPVPMNQNPGL-- 300

Query: 301 PLPFGFPPFPTE---GAQQYYS-TPYEVPRQDTAAETGSEQGSLLGF-PPGAAGGMNFSA 360
           P  FGFP +PT     +QQ+++   YE+ R ++    GSEQ SLLG+ PP     +NF  
Sbjct: 301 PQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYPPPSPMTNLNFQG 330

Query: 361 TYPQP-WNTGQA 362
           +YP P WN G A
Sbjct: 361 SYPPPSWNPGMA 330

BLAST of Cp4.1LG08g12030 vs. NCBI nr
Match: XP_023539354.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 928 bits (2398), Expect = 0.0
Identity = 471/495 (95.15%), Postives = 477/495 (96.36%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL---KRFYMLPVSKNALKVLKWLLC--CDDRIQKI 120
           RSRRHLGERVHFCVRCDFPIAVYGRL   +  + L  +++        +C  CDDRIQKI
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDS------ICYLCDDRIQKI 120

Query: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180
           QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST
Sbjct: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH
Sbjct: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
           GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP
Sbjct: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300

Query: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360
           PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP
Sbjct: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360

Query: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420
           WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS
Sbjct: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420

Query: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480
           MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW
Sbjct: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480

Query: 481 QNDSHSRDSFGNGQD 490
           QNDSHSRDSFGNGQD
Sbjct: 481 QNDSHSRDSFGNGQD 489

BLAST of Cp4.1LG08g12030 vs. NCBI nr
Match: XP_022935351.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita moschata] >XP_022935435.1 E3 ubiquitin-protein ligase Hakai-like [Cucurbita moschata])

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 462/495 (93.33%), Postives = 469/495 (94.75%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL---KRFYMLPVSKNALKVLKWLLC--CDDRIQKI 120
           RSRRHLGERVHFCVRCDFPIAVYGRL   +  + L  +++        +C  CDDRIQKI
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDS------ICYLCDDRIQKI 120

Query: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180
           QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST
Sbjct: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASESTVRG SRPIISPS NS AQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH
Sbjct: 181 ASESTVRGLSRPIISPSFNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
           G FPPQNFDVQGTPHQESSQFSEK QGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP
Sbjct: 241 GRFPPQNFDVQGTPHQESSQFSEKPQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300

Query: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360
           PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP
Sbjct: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360

Query: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420
           WNTGQAGVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS
Sbjct: 361 WNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420

Query: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480
           MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW
Sbjct: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480

Query: 481 QNDSHSRDSFGNGQD 490
           QNDSHSRDSFG+GQD
Sbjct: 481 QNDSHSRDSFGSGQD 489

BLAST of Cp4.1LG08g12030 vs. NCBI nr
Match: KAG6596366.1 (E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 461/495 (93.13%), Postives = 468/495 (94.55%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL---KRFYMLPVSKNALKVLKWLLC--CDDRIQKI 120
           RSRRHLGERVHFCVRCDFPIAVYGRL   +  + L  +++        +C  CDDRIQKI
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDS------ICYLCDDRIQKI 120

Query: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180
           QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST
Sbjct: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASESTVRG SRPIISPSSNS AQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH
Sbjct: 181 ASESTVRGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
           G FPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP
Sbjct: 241 GRFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300

Query: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360
           PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QP
Sbjct: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQP 360

Query: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420
           WNTGQAGVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS
Sbjct: 361 WNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420

Query: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480
           MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLP YLSHNKRGKFHSGDTDHDGQG GW
Sbjct: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGW 480

Query: 481 QNDSHSRDSFGNGQD 490
           QNDSHSRDSFG+GQD
Sbjct: 481 QNDSHSRDSFGSGQD 489

BLAST of Cp4.1LG08g12030 vs. NCBI nr
Match: XP_022971545.1 (E3 ubiquitin-protein ligase Hakai-like [Cucurbita maxima] >XP_022971546.1 E3 ubiquitin-protein ligase Hakai-like [Cucurbita maxima])

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 456/495 (92.12%), Postives = 464/495 (93.74%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL---KRFYMLPVSKNALKVLKWLLC--CDDRIQKI 120
           RSRR LGERVHFCVRCDFPIAVYGRL   +  + L  +++        +C  CDDRIQKI
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDS------ICYLCDDRIQKI 120

Query: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180
           QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHA+LLKPNAE EDGNEIEALSAKQST
Sbjct: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHAELLKPNAEMEDGNEIEALSAKQST 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASESTVRGPSRPIISPSSNS AQE DEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH
Sbjct: 181 ASESTVRGPSRPIISPSSNSQAQEHDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
           G FPPQNFDVQG PHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP
Sbjct: 241 GRFPPQNFDVQGPPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300

Query: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360
           PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP
Sbjct: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360

Query: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420
           WNTGQAGVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS
Sbjct: 361 WNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420

Query: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480
           MDLGH GNAMDLKNGRGILAPQPPLAQLPLPPP P YLSHNK GKFHSGDTDHDGQGHGW
Sbjct: 421 MDLGHYGNAMDLKNGRGILAPQPPLAQLPLPPPRPPYLSHNKHGKFHSGDTDHDGQGHGW 480

Query: 481 QNDSHSRDSFGNGQD 490
           QNDSHSRDSFG+GQD
Sbjct: 481 QNDSHSRDSFGSGQD 489

BLAST of Cp4.1LG08g12030 vs. NCBI nr
Match: KAG7027916.1 (hypothetical protein SDJN02_09095 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884 bits (2284), Expect = 0.0
Identity = 453/490 (92.45%), Postives = 454/490 (92.65%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRLKRFYMLPVSKNALKVLKWLLCCDDRIQKIQTIKL 120
           RSRRHLGERVHFCVRCDFPIAVYGRLK                           IQTIKL
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLK---------------------------IQTIKL 120

Query: 121 MEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASEST 180
           MEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASEST
Sbjct: 121 MEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTASEST 180

Query: 181 VRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGHFPP 240
           VRG SRPIISPSSNS AQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHG FPP
Sbjct: 181 VRGLSRPIISPSSNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPHGRFPP 240

Query: 241 QNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFG 300
           QNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFG
Sbjct: 241 QNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSPPLPFG 300

Query: 301 FPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQPWNTGQ 360
           FPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATY QPWNTGQ
Sbjct: 301 FPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYSQPWNTGQ 360

Query: 361 AGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGH 420
           AGVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGH
Sbjct: 361 AGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKSMDLGH 420

Query: 421 SGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGWQNDSH 480
           SGNAMDLKNGRGILAPQPPLAQLPLPPPLP YLSHNKRGKFHSGDTDHDGQG GWQNDSH
Sbjct: 421 SGNAMDLKNGRGILAPQPPLAQLPLPPPLPPYLSHNKRGKFHSGDTDHDGQGRGWQNDSH 463

Query: 481 SRDSFGNGQD 490
           SRDSFG+GQD
Sbjct: 481 SRDSFGSGQD 463

BLAST of Cp4.1LG08g12030 vs. ExPASy TrEMBL
Match: A0A6J1FAM2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111442234 PE=4 SV=1)

HSP 1 Score: 906 bits (2341), Expect = 0.0
Identity = 462/495 (93.33%), Postives = 469/495 (94.75%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL---KRFYMLPVSKNALKVLKWLLC--CDDRIQKI 120
           RSRRHLGERVHFCVRCDFPIAVYGRL   +  + L  +++        +C  CDDRIQKI
Sbjct: 61  RSRRHLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDS------ICYLCDDRIQKI 120

Query: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180
           QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST
Sbjct: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASESTVRG SRPIISPS NS AQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH
Sbjct: 181 ASESTVRGLSRPIISPSFNSQAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
           G FPPQNFDVQGTPHQESSQFSEK QGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP
Sbjct: 241 GRFPPQNFDVQGTPHQESSQFSEKPQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300

Query: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360
           PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP
Sbjct: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360

Query: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420
           WNTGQAGVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS
Sbjct: 361 WNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420

Query: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480
           MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW
Sbjct: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480

Query: 481 QNDSHSRDSFGNGQD 490
           QNDSHSRDSFG+GQD
Sbjct: 481 QNDSHSRDSFGSGQD 489

BLAST of Cp4.1LG08g12030 vs. ExPASy TrEMBL
Match: A0A6J1I614 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470230 PE=4 SV=1)

HSP 1 Score: 894 bits (2310), Expect = 0.0
Identity = 456/495 (92.12%), Postives = 464/495 (93.74%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPLQAETVTVACPDHLILADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLQAETVTVACPDHLILADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL---KRFYMLPVSKNALKVLKWLLC--CDDRIQKI 120
           RSRR LGERVHFCVRCDFPIAVYGRL   +  + L  +++        +C  CDDRIQKI
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAVYGRLSPCEHVFCLDCARSDS------ICYLCDDRIQKI 120

Query: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQST 180
           QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHA+LLKPNAE EDGNEIEALSAKQST
Sbjct: 121 QTIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHAELLKPNAEMEDGNEIEALSAKQST 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASESTVRGPSRPIISPSSNS AQE DEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH
Sbjct: 181 ASESTVRGPSRPIISPSSNSQAQEHDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
           G FPPQNFDVQG PHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP
Sbjct: 241 GRFPPQNFDVQGPPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300

Query: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360
           PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP
Sbjct: 301 PLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPPGAAGGMNFSATYPQP 360

Query: 361 WNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420
           WNTGQAGVPYEHAAGGQGMPD FANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS
Sbjct: 361 WNTGQAGVPYEHAAGGQGMPDAFANAPDSQVNHGFYQGDYRRSPGGLPVNSSVSSMANKS 420

Query: 421 MDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKRGKFHSGDTDHDGQGHGW 480
           MDLGH GNAMDLKNGRGILAPQPPLAQLPLPPP P YLSHNK GKFHSGDTDHDGQGHGW
Sbjct: 421 MDLGHYGNAMDLKNGRGILAPQPPLAQLPLPPPRPPYLSHNKHGKFHSGDTDHDGQGHGW 480

Query: 481 QNDSHSRDSFGNGQD 490
           QNDSHSRDSFG+GQD
Sbjct: 481 QNDSHSRDSFGSGQD 489

BLAST of Cp4.1LG08g12030 vs. ExPASy TrEMBL
Match: A0A6J1CWS1 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111015005 PE=4 SV=1)

HSP 1 Score: 783 bits (2021), Expect = 2.74e-282
Identity = 407/512 (79.49%), Postives = 433/512 (84.57%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKPL AETVTVACPDHL+LADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPLPAETVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRLK----RFYMLPVSKNALKVLKWLLCCDDRIQKIQ 120
           RSRR LGERVHFCVRCDFPIA+YGRL      F +     +++  L     CDDRIQKIQ
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSICYL-----CDDRIQKIQ 120

Query: 121 TIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTA 180
           TIKLMEGIFICAAPHCLKSFLKRTEFESHI ESHADLLKPNA+KEDGNEIEA SAKQSTA
Sbjct: 121 TIKLMEGIFICAAPHCLKSFLKRTEFESHIHESHADLLKPNADKEDGNEIEAHSAKQSTA 180

Query: 181 SESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQ----------------Q 240
           SEST RGPSRPIISP SNS  QER+EKFHRQQSRDQP SGMQQ                Q
Sbjct: 181 SESTARGPSRPIISPGSNSQLQEREEKFHRQQSRDQPRSGMQQKQAPNFGQNQNNPSESQ 240

Query: 241 DSGGHSQGFDRHGPHGHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPP 300
              GHSQGFDRHGPHG FPPQNFD+QG PHQES+QFS+KQQGIL D P+SQYPPLQ IPP
Sbjct: 241 PDSGHSQGFDRHGPHGRFPPQNFDLQGGPHQESNQFSDKQQGILTDTPFSQYPPLQPIPP 300

Query: 301 PNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAAETGSEQGSLLGFPP 360
           PNYVVP NSNPML+PPLPFG+PPFP EGAQQYYSTPYEV RQD A ++GSEQGSLLGFPP
Sbjct: 301 PNYVVPTNSNPMLTPPLPFGYPPFPIEGAQQYYSTPYEVSRQDAAVDSGSEQGSLLGFPP 360

Query: 361 GAAGGMNFSATYPQPWNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSPG 420
           GAAGGMNFSATYPQ WNTGQAG PY+HAAG QGMPD+F+N PDSQ  HGFYQGDYRRSPG
Sbjct: 361 GAAGGMNFSATYPQSWNTGQAGAPYDHAAGSQGMPDSFSNTPDSQGKHGFYQGDYRRSPG 420

Query: 421 GLPVNSSVSSMANKSMDLGHSGNAMDLKNGRGILAPQPPLAQLPLPPPLPL--YLSHNKR 480
           GLPVNSSVSSMANK MDLGH+G AMD + G+GILAPQP + QLPLPPP P   YLS +KR
Sbjct: 421 GLPVNSSVSSMANKGMDLGHAGTAMDFREGKGILAPQPTM-QLPLPPPPPPPPYLSQHKR 480

Query: 481 GKFHSGDTDHDGQGHGWQNDSHSRDSFGNGQD 490
           GKF+SGD DHDGQG  WQNDSHSRDSFG+GQD
Sbjct: 481 GKFYSGDMDHDGQGLPWQNDSHSRDSFGSGQD 506

BLAST of Cp4.1LG08g12030 vs. ExPASy TrEMBL
Match: A0A6J1J5I8 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 PE=4 SV=1)

HSP 1 Score: 778 bits (2010), Expect = 1.29e-280
Identity = 406/512 (79.30%), Postives = 433/512 (84.57%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKP  AE+VTVACPDHL+LADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRLK----RFYMLPVSKNALKVLKWLLCCDDRIQKIQ 120
           RSRR LGERVHFCVRCDFPIA+YGRL      F +     ++L  L     CDDRIQKIQ
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYL-----CDDRIQKIQ 120

Query: 121 TIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTA 180
           TIK+MEGIFICAAPHCLKSFLKRT+FESHI ESHADLLKPNAEKEDGNEIEA SAKQSTA
Sbjct: 121 TIKMMEGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTA 180

Query: 181 SESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQ----------------Q 240
           SE++VR P RP+ISP SNS  QER+EKFHRQQSRDQP SGMQQ                Q
Sbjct: 181 SETSVRCPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQINTSESQ 240

Query: 241 DSGGHSQGFDRHGPHGHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPP 300
              GHSQGFDRHGPHG FPPQNFD QG PHQESSQFSEKQQGIL+D PYSQYPPLQ IPP
Sbjct: 241 QDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPP 300

Query: 301 PNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAA-ETGSEQGSLLGFP 360
           PNYVVP NSNPML+PPLPFG+PPFP EGAQQYYSTPYEVPRQDTAA ETGSEQGSLLGFP
Sbjct: 301 PNYVVPPNSNPMLTPPLPFGYPPFPVEGAQQYYSTPYEVPRQDTAAAETGSEQGSLLGFP 360

Query: 361 PGAAGGMNFSATYPQPWNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSP 420
           PGAAGGMNFSATYP PWN GQAG+PY+HAAGGQGMPD FAN PDSQ  HGFY GDYRRSP
Sbjct: 361 PGAAGGMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSP 420

Query: 421 GGLPVNSSVSSMANKSMDLGH-SGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKR 480
           GG+PVNS+VSSMANK MD GH SG+ MDL++G+GILAPQP L QLP PP  P +LSHNKR
Sbjct: 421 GGMPVNSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQP-LIQLPPPPRPPPFLSHNKR 480

Query: 481 GKFHSGDTDHDGQGHGWQNDSHSRDSFGNGQD 490
           GKF+ GDTDHDGQ  GWQND+HSRDSFG+GQD
Sbjct: 481 GKFYPGDTDHDGQNLGWQNDNHSRDSFGSGQD 506

BLAST of Cp4.1LG08g12030 vs. ExPASy TrEMBL
Match: A0A6J1F6T3 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395 PE=4 SV=1)

HSP 1 Score: 776 bits (2005), Expect = 7.46e-280
Identity = 404/512 (78.91%), Postives = 433/512 (84.57%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRLSKVPQAEGGAG VKP  AE+VTVACPDHL+LADLPVA GIGAATAASIVKSVGR
Sbjct: 1   MLQIRLSKVPQAEGGAGAVKPSPAESVTVACPDHLVLADLPVAKGIGAATAASIVKSVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRLK----RFYMLPVSKNALKVLKWLLCCDDRIQKIQ 120
           RSRR LGERVHFCVRCDFPIA+YGRL      F +     ++L  L     CDDRIQKIQ
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLSPCEHAFCLDCARSDSLCYL-----CDDRIQKIQ 120

Query: 121 TIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSAKQSTA 180
           TIK+MEGIFICAAPHCLKSFLKRT+FESHI ESHADLLKPNAEKEDGNEIEA SAKQSTA
Sbjct: 121 TIKMMEGIFICAAPHCLKSFLKRTDFESHIHESHADLLKPNAEKEDGNEIEAHSAKQSTA 180

Query: 181 SESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQ----------------Q 240
           SE++VRGP RP+ISP SNS  QER+EKFHRQQSRDQP SGMQQ                Q
Sbjct: 181 SETSVRGPLRPLISPGSNSQPQEREEKFHRQQSRDQPRSGMQQKQAPILGQNQNNASESQ 240

Query: 241 DSGGHSQGFDRHGPHGHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPP 300
              GHSQGFDRHGPHG FPPQNFD QG PHQESSQFSEKQQGIL+D PYSQYPPLQ IPP
Sbjct: 241 QDSGHSQGFDRHGPHGRFPPQNFDAQGAPHQESSQFSEKQQGILSDTPYSQYPPLQPIPP 300

Query: 301 PNYVVPANSNPMLSPPLPFGFPPFPTEGAQQYYSTPYEVPRQDTAA-ETGSEQGSLLGFP 360
           PNYVVP NSNPML+PPLPFG+PPFP EGAQ+YYS+PYEVPRQDTAA ETGSEQGSLLGFP
Sbjct: 301 PNYVVPPNSNPMLTPPLPFGYPPFPVEGAQKYYSSPYEVPRQDTAAAETGSEQGSLLGFP 360

Query: 361 PGAAGGMNFSATYPQPWNTGQAGVPYEHAAGGQGMPDTFANAPDSQVNHGFYQGDYRRSP 420
           PGAAGGMNFSATYP PWN GQAG+PY+HAAGGQGMPD FAN PDSQ  HGFY GDYRRSP
Sbjct: 361 PGAAGGMNFSATYPHPWNAGQAGIPYDHAAGGQGMPDAFANTPDSQGKHGFYPGDYRRSP 420

Query: 421 GGLPVNSSVSSMANKSMDLGH-SGNAMDLKNGRGILAPQPPLAQLPLPPPLPLYLSHNKR 480
           GG+PVNS+VSSMANK MD GH SG+ MDL++G+GILAPQP L QLP PP  P +LSHNKR
Sbjct: 421 GGMPVNSTVSSMANKGMDQGHASGSTMDLRDGKGILAPQP-LIQLPPPPRPPPFLSHNKR 480

Query: 481 GKFHSGDTDHDGQGHGWQNDSHSRDSFGNGQD 490
           GKF+ GDTDHD Q  GWQND+HSRDSFG+GQD
Sbjct: 481 GKFYPGDTDHDAQNLGWQNDNHSRDSFGSGQD 506

BLAST of Cp4.1LG08g12030 vs. TAIR 10
Match: AT5G01160.1 (RING/U-box superfamily protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.8e-66
Identity = 172/372 (46.24%), Postives = 222/372 (59.68%), Query Frame = 0

Query: 1   MLQIRLSKVPQAEGGAGTVKPLQAETVTVACPDHLILADLPVANGIGAATAASIVKSVGR 60
           MLQIRL +    E G G  +P   ETVTVACPDHL+LADLPVA GIG+ T  +++K VGR
Sbjct: 1   MLQIRLRRDSPTETGNG-ARPSPTETVTVACPDHLVLADLPVAKGIGSVTPTTVIKPVGR 60

Query: 61  RSRRHLGERVHFCVRCDFPIAVYGRL----KRFYMLPVSKNALKVLKWLLCCDDRIQKIQ 120
           RSRR LGERVHFCVRCDFPIA+YGRL      F +     +++  L     CD+RIQKIQ
Sbjct: 61  RSRRQLGERVHFCVRCDFPIAIYGRLIPCDHAFCLECARSDSICYL-----CDERIQKIQ 120

Query: 121 TIKLMEGIFICAAPHCLKSFLKRTEFESHIQESHADLLKPNAEKEDGNEIEALSA-KQST 180
           TIK+MEGIFICAAPHCL+SFLK+ +FE+H+ + H  LL+ +AEKEDGN+ +  S  +QS+
Sbjct: 121 TIKMMEGIFICAAPHCLRSFLKKLDFEAHVHDLHGSLLQADAEKEDGNQSDVQSTMQQSS 180

Query: 181 ASESTVRGPSRPIISPSSNSLAQERDEKFHRQQSRDQPTSGMQQQDSGGHSQGFDRHGPH 240
           ASEST+R P R  +                 QQSR+   S    +   G SQ        
Sbjct: 181 ASESTLRAPLRSQL-----------------QQSRELNRSASFAKSQSGFSQ-------V 240

Query: 241 GHFPPQNFDVQGTPHQESSQFSEKQQGILADPPYSQYPPLQSIPPPNYVVPANSNPMLSP 300
            ++PP + D    P  E+   +  + GI   P Y Q  P+  + PP+  VP N NP L  
Sbjct: 241 QNYPPDS-DNSRPPGFET---ASPKPGI-RFPDYPQ--PMNLMQPPSLPVPMNQNPGL-- 300

Query: 301 PLPFGFPPFPTE---GAQQYYS-TPYEVPRQDTAAETGSEQGSLLGF-PPGAAGGMNFSA 360
           P  FGFP +PT     +QQ+++   YE+ R ++    GSEQ SLLG+ PP     +NF  
Sbjct: 301 PQQFGFPSYPTTESGSSQQFFNGAQYEMTRTESG---GSEQSSLLGYPPPSPMTNLNFQG 330

Query: 361 TYPQP-WNTGQA 362
           +YP P WN G A
Sbjct: 361 SYPPPSWNPGMA 330

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LFC02.5e-6546.24E3 ubiquitin-protein ligase HAKAI homolog OS=Arabidopsis thaliana OX=3702 GN=HAK... [more]
Match NameE-valueIdentityDescription
XP_023539354.10.095.15E3 ubiquitin-protein ligase Hakai-like [Cucurbita pepo subsp. pepo][more]
XP_022935351.10.093.33E3 ubiquitin-protein ligase Hakai-like [Cucurbita moschata] >XP_022935435.1 E3 u... [more]
KAG6596366.10.093.13E3 ubiquitin-protein ligase HAKAI-like protein, partial [Cucurbita argyrosperma ... [more]
XP_022971545.10.092.12E3 ubiquitin-protein ligase Hakai-like [Cucurbita maxima] >XP_022971546.1 E3 ubi... [more]
KAG7027916.10.092.45hypothetical protein SDJN02_09095 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1FAM20.093.33RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111442234... [more]
A0A6J1I6140.092.12RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111470230 P... [more]
A0A6J1CWS12.74e-28279.49RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101500... [more]
A0A6J1J5I81.29e-28079.30RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111481547 P... [more]
A0A6J1F6T37.46e-28078.91RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441395... [more]
Match NameE-valueIdentityDescription
AT5G01160.11.8e-6646.24RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 244..259
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 458..476
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..197
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..259
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 210..226
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 439..490
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..167
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 322..341
NoneNo IPR availablePANTHERPTHR13480:SF0E3 UBIQUITIN-PROTEIN LIGASE HAKAIcoord: 1..457
IPR040383E3 ubiquitin-protein ligase HAKAI/CBLL2PANTHERPTHR13480E3 UBIQUITIN-PROTEIN LIGASE HAKAI-RELATEDcoord: 1..457
IPR013087Zinc finger C2H2-typePROSITEPS00028ZINC_FINGER_C2H2_1coord: 127..150
IPR013087Zinc finger C2H2-typePROSITEPS50157ZINC_FINGER_C2H2_2coord: 125..151
score: 8.828978

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g12030.1Cp4.1LG08g12030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0030155 regulation of cell adhesion
molecular_function GO:0046872 metal ion binding
molecular_function GO:0061630 ubiquitin protein ligase activity