Cp4.1LG08g10590 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g10590
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptiondelta(14)-sterol reductase-like
LocationCp4.1LG08: 8019156 .. 8025575 (-)
RNA-Seq ExpressionCp4.1LG08g10590
SyntenyCp4.1LG08g10590
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGCCCAGCCATTCCCGCCTGCTGTGGAGATTTACAAGCTAGCAGAGCAGTTGAACCAGCGCCTGTGGATTTAGTAGCTGCAATGAACAGACTCGGGGCTCGAGCTAAAGGCAGCCATAGTTGAAATTAGACAATCCCGAGAAGAAATTAGTTCCAATTTTGCAGCAAATTATCATTGTTACCAGAGGAAACAAGTCGATCGAGCGACAAAATCCGAACAAGTTGTCAATGATCGATTTCTTGAGGAATATGGATCTGGAGTTTCTTCTTCAACTTGCAATCCCTTCTTGGGAAGCTGTAAGTGCTAATTCTTTTGTATAAGTTTGATTTAGACTTCAATGTGTTTGAGATTCTTCCTGCTGTATGTTGTTGGATAATCAGGTTGCTATACTTTCCATTTTCTTCTTTTACTTGGCGATTGCTGGCTCTGTTCTACCTGGAAGGATTATCCCAGGAGTGATCTTGTCGGACCGGACTAGGCTTCATTATCGCTGCAATGGTATCGTTATCTCATATGTAAATGCAGTTTGTTAAGTTTGATATCACTGAGATCATCATTTCTTGGCAAATTAGTACTAAAATTTGGATTCCTCTTGGGGTTCTTTTGTGCTGCTATAGGTCTACTCTCTCTTCTGGTGCTTCTTGCACTTCTTGGGATTGGTGTTAAGATGAATTACATTTCGCCCACTGTGAGTGTTCATTGATTTTCGTATGTGTTGGATCACTCTACTTATTTGGCTCTACAGAAAATTTTTTTATTGAAATTTTGTATTAGTATTTCTTCCTTGATGTTGGTAACTGTCCTTATTAGATGCTTATAATACAACTCATATGTAGGCAATTTCAGATAGAGGGGTGGAGCTGTTATCCGCAACCTTCTGGTTTAGTGTCCTGGTATGTGAATTTCAAATTGAATATCTGAACTATTTGCTATGCAGCTGATATTATCAACGTTACTTGCTGACATTTTTCTGCATCGAACTTTTGCACATCACAACATCCCTGTTGACTTTTCACGCCTACCTTTTTCCTTTTGAGATTTGAGTGGTGGTGATAGAGACTAATTCTTGTTGATAACAAAGAGGTACAAGGCAAGGGTGTAACGACACATGGCCAGAATTTGGAATCCGAATTCGGCACTTGATGGCCCCAACATTCTCTTGCATCATGGTCACGTTACTTACTCCTCGCTTCTTAGCGTGAAGACTATCTCCACAGATCAACCCGAGTTTCTCCAGCATGTTTTGTCTTGACTCGTATGCATCTTAGAAAAATTCCCAGGAGGATACCCAACATAGAATTGCTCCAAGCAAAACATAAGTTCTTATGATTGAGCTACCATAAAAGAAAGTGCACCTTGTTGGTATAGTTAGTACTTTTAAATTCTTTAAATCTTTCTTAGACATCCTATCTTGAGGATTGCTCTCATTCGAATGTGGTCTCAGTTTATTCATGTATTGTTCCTTTACTCGGGTGTCGCAAAGGGGGATTTCGAGAACCCCTATCTCTTCTAAGCCAAATGCATAACTAACCAAAACCACATATACCTCTAAGCCAAAGCTTTTATTTGTATGCTTCCTCATATTTACTCAAGCTTACATCATAATCGTCACATGGACCCCACTACAACAATCCTCACACACACTTTTATATCATTTTCTGCCCCCTCATTCTCTTTGTAGTTTTGACATTAAGCATTGGGGGTCGATGAAGTGGTTCTATCATGTTGACAAGCTTCCGTATGGATAACTATTTCTGCACCTAAACGCAAAACTTCTTGTCCCTCAGCGATAAATAGTTGGTTAGTGTAGAATACGCTTGTTTGTTTCTTAAGATATCCCATTGTAATTCTGAAAGGGCTTTATGTTATAAGAGGAATTCAATTGGGGATTCTGTTGATTTGTAACTTCCTAGGAACCACTTGGGAAGATTTCCTACAATGACCATCAATAAGCTTGTTTCTAGGCCCCCAAATTCAAATCATCATGCAAGAATTGATGATTATTCGATTTCAATAATTAAAACACAATACCAGTACCATGATTCTTGCACTATGTGGTTATTCTTATAACATGTCCTTTTAACTATTTGTTAAGTTTGGTCTCTGAATCTTCTGGTCATCTATTATCAATTACTTCTCTATTTCTAGCTCTCTTTTTTTAACCTTCTCTTTAATGTCTCACTTTCAGGCAACATTTTTACTTTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTTCTTCTCTAAAGCCCTGTATCACAGGGAACTTTATTCACGATTGGTACATATTCAATTTCGGTTCCTTTCTCTGTATATGTATAATGTCACTGCTTCTCCTTTTGGCTTTTAGTTTTTGAAAGTTTGAAAAAAGAGGCATAATTTTTTTGTTTGCTAAATTCTTGAAGTCTGATTTGACCACTTATCTAAGTATCAGCTTTTTTTGCTGCTTAACAGGTGGTTTGGTATACAGTTGAATCCTCACTTTTTGGGCATTGATCTCAAGTATGTTACTTATTTAGAACTTTATCACGTGCCATTCAGGAATAATGTTATTAGTTTAAACGCCACTTCTTATGCAACGATGCTCTCAGCCGTTTACATTTATTGATTTTCAATATGCTTATTATGTTCACTAACAGATGGTCCTTTGCACCGATTTCATATTGACAAGAATGCTTTAAGTCCTCCTGTAGATTTTTCTTTGTGAGAGCTGGAATGATGGGGTGGCTACTTATTAACCTATCAGTTCTTGCCAGAAGTGTTTTGGACGCCACCCTAAGCCAATCAATGATTCTCTACCAGACATTTTGTGCGGTAAACTGATAGTTTGTATTCCATCATTTTTTTTCCTTTTATTTTGGTGGCATCTAATGGTTGGATGTTTGGTCTAATATGTTGATTCAGTTATATGTCCTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACGTAAGTGTATTTTCAGATCATTCTTTCCTGTTGTTTGAAACATGTTTTGAAATGCTTCTGAGCTCGGTCTCTTGATTGAATAAGAGCCAAGGATCTTCAACATCAACAAGTCTGCCAATGAAAAGTAATGAATATAAACCGTGACCAATTCCTTGGACACTAGGGAAATAAAATTGTGAATGGCATCCGTTAAAATGTTCTAGCAGAAGCTTATATGTTGCTACTCTTGGATACCGGTGCTCATTGAATCTCGGTTATGTTATTCTTATTATGACTATTGTCTGTCTGAGGTAGTTGGAGTCTCTGTATTTTATCCATATTAGCCGGCTTCTAAAACATTTTTTAGCCTAACTGGTTACTTGGAGACAGTCATGAATCTGGTTCTGCCCTTCATCAACTCTCTAATCAGCAACCGAATTACCTTGTACGAAAAACGTGTTATTTCAAAAGACAGGTTTCTGGTGAATAATAGCTCTGTTTGTATAACATAAAATAGTCTCCATTAGATTTTGTGCCTGCCATTATGAATTCTTAATTGCCATTTTCATATGAACTGTGGTCATTATACATTTTTATGCTTGGAGCACTCGCTTCATTTCACTTGCGATCTTGTGCAATCAGACTGAATATTAAGGCATGATTTATCTTGTGTAGATGGGACATAATTGCTGAAAGATTAGGCTTCATGCTGGTGTTTGGAGATCTTGTTTGGATTCCTTTTACATTTAGCATTCAGGTAAATAATGAAGTAGTTTACCAGTTTCTCTACTGTCACTCAAGCATTCGCTTTCCCTTGATCTTCATTAAACTAGTGAGAAGCTGGAGAGAGGAACAAAATATTCCTTTTAAGGGTGTGGAAACCTCTTCCGAACAGACACATTTAAAACCTTGGGGGAGCCAAGGAAAAGCCCAAAAAGGACAATATCTATTAGTGGTGGACTTGGGCCATTACAAATGGTATAAGAGCCAGACACCAAGCGGTGTGCCAGTGAGGACAGTGGGTCTCCAAGGGGGGTGGGCTGTGACATCCCACATCGGTTGGAGAAGGGAACGAAGCATTCCTTATAAGGGTGTGGAAACCTCTCCCAAATAGATACATTTTAAAATTTCGAGGGAAACCCAGAAGGGAAAGCCGAAAGAGGACAATATCTGCTAGCGGTGGGCTTGGGCTGTTACACAAGCGCAAAACCTCCTTTAGGAATATAGAAAGATCACTCCATAGATGAAGTAAACAGAAAAATTATAACAACCCTTGGTAAAATATAAGAAAGCATCCCCACTAGAAAATCATTCACAAATTGTTCTTCTTCCACACAAAGAAACCTCCTCGAAATACAAAAACCCCCATGAAAGCATAACCAAAAAGAATATTAAAGAGCTTAGAAGTTTGAATCCTCAACTCCACATGTTGAACTAACAAAAAAGAATACCCGAGCTTCCCACAGACCTCCTTCTATCTTTTTCAATCTAGGAGGCTATTCCCCAGACTTTCATCTGCTTATTAAAAGCCATCTCCTTGTGGATAAAAGAAAGGAATTCAAGATCATCGTCAAGTTCCGCTGTATTATACTTGCACTATTAGTCATCTTGTTGACTAAAACTTTTGCATGGAACCAGGATCATTATCGCACTTGCTCTGATATTGTTTGTATTTTGTGTCCAAATGGATTCTTAATTCTCCAATTTCAAACTGAAACTCACCCATATGAGTTCAGATTGTCTTGCGCATTTACCATCACAAATTCTGGACCATTATCTTCATCCGTCAATTTATCTTTTAGAGCTTAATGTAGGCTCTATGTTAAACTATCTGTATATTGTAAGTAGTAATGGATCGTAAGTTTGTTTTCATATTCAGATTATCTGTGTTCGCTTTTGTTATAGGGTTGGTGGCTTTTAACAAACAAGGCTGAGCTAACAACAGCTGCTACCATAGCTAACTGCTCGGTCTTTTTAATCGGGTAGCTATCACGACGTGACATAACTTATCATTGACATTTTCTTTTTCCTTGTCTTGAAGGTTCACTAAGATGTGAGATTTTTGTGTACTAATTTGGGCAGATATCTTGTGTTTAGAGGATCTAACAAGCAAAAACACATGTTTAAAAAGAACAACAAAGCGCTTATATGGGGTAGGCCTCCCAAAGTTGTAGGAGGAAAGTTGCTTGCTTCTGGTTATTGGTAAGGTTTTCATCTATTTTTGTTTATTTGTTGTCATAGGCATAACGTCTCATTAAGTGTTTTGAGGGGAACCGAATTTTTCTGATAGCTTTTTAGGGCTATACTGCAATGTTCTCATTTCTTGTTTTTCCGAAGTGTGTTTGATAATGGTAACGTTTCTTGTTCTCTCAATACGTTAAAGTTTTTCTCGGAAATAATACTTCAGAATAAATTCTTCAAATTTTATACACAAATTTAGAGACTCTTAAAACTGACAAACAAGAAACGTAAAACGATCAATCCATGAACACGTGTTTCTTAGAAACTAAATCCGAGACTCCAGTTCCGGTTAATTGTGCTTGTCAGCCTGAGGCCTGTTGAGATCTTACATTTTGCAAATCATGGGTATTGTTTGGACACCATTTCTCAGGACATGGCAGGCCACCCTATCACTAAAAGGTGGATCAATTTCAATCCACTTTTATTTCCTCGTACTCATCTGAAATAACTGTTATTGGTGTCGTAAGGCGACGCAATCCCGGTGTCTCGTCACTTTGACCCTCTGTTCACGTCGCTCGTTCTTCACGGCCTAAAAATCTAATACTTGCTGTTGAAACAGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTACCATGTGGGATAAGGTAATTTATTGGTTCCTCTTTCTTTGTAGCCAAAAGAAAAGAAAGAAGAAGAAATCTGATAATCCCGTCATTTTTGCAGCTCCCCAATTCCATACTTTTATCCCACATATCTCCTCCTTCTGCTAATATGGAGAGAAAGACGGGATGAAGCTCGATGCGCTGAGAAATATAAAGACATTTGGGCGGAGTATACCAAACTCGTCCCTTGGAGAATACTGCCTTATGTTTATTAGCATAGACAGATGTTGTTACTTGTGTGAAATATGAACACTCTCGTCGCCGAGATTTTCGTCGTCTTCTTGTCTGGTGCTTCTCTTCTCTTCTTCCTGAGGACACAACTTTCATCTGCTTCCCATGTTCATGGATTGTCTGCATTTTACAAGGTCTGTGTTAAGTTATTCTTGTCTGCTTATTGTGTCTTTAACTTGGTTAACCTGCACTTCATTTTCTAGATTTAGAAATTCTTGTGACAATGGGAATAAATAATCTCTCCTTTATTATCATCTCTCTCTTTCTCTCCATTTTTTTGTTTCCTTTATTGGTCTTAGTTTTGATAAAATTAATAGAATTGAGCAGCTGGTGACATGAACTTGACTTTGAGTCTCCGAGGCTATA

mRNA sequence

CAGCCCAGCCATTCCCGCCTGCTGTGGAGATTTACAAGCTAGCAGAGCAGTTGAACCAGCGCCTGTGGATTTAGTAGCTGCAATGAACAGACTCGGGGCTCGAGCTAAAGGCAGCCATAGTTGAAATTAGACAATCCCGAGAAGAAATTAGTTCCAATTTTGCAGCAAATTATCATTGTTACCAGAGGAAACAAGTCGATCGAGCGACAAAATCCGAACAAGTTGTCAATGATCGATTTCTTGAGGAATATGGATCTGGAGTTTCTTCTTCAACTTGCAATCCCTTCTTGGGAAGCTGTTGCTATACTTTCCATTTTCTTCTTTTACTTGGCGATTGCTGGCTCTGTTCTACCTGGAAGGATTATCCCAGGAGTGATCTTGTCGGACCGGACTAGGCTTCATTATCGCTGCAATGGTCTACTCTCTCTTCTGGTGCTTCTTGCACTTCTTGGGATTGGTGTTAAGATGAATTACATTTCGCCCACTGCAATTTCAGATAGAGGGGTGGAGCTGTTATCCGCAACCTTCTGGTTTAGTGTCCTGGCAACATTTTTACTTTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTTCTTCTCTAAAGCCCTGTATCACAGGGAACTTTATTCACGATTGGTGGTTTGGTATACAGTTGAATCCTCACTTTTTGGGCATTGATCTCAAATTTTTCTTTGTGAGAGCTGGAATGATGGGGTGGCTACTTATTAACCTATCAGTTCTTGCCAGAAGTGTTTTGGACGCCACCCTAAGCCAATCAATGATTCTCTACCAGACATTTTGTGCGTTATATGTCCTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACAAGCTTATATGTTGCTACTCTTGGATACCGGTGCTCATTGAATCTCGGTTATGTTATTCTTATTATGACTATTGTCTGTCTGAGATGGGACATAATTGCTGAAAGATTAGGCTTCATGCTGGTGTTTGGAGATCTTGTTTGGATTCCTTTTACATTTAGCATTCAGGGTTGGTGGCTTTTAACAAACAAGGCTGAGCTAACAACAGCTGCTACCATAGCTAACTGCTCGGTCTTTTTAATCGGATATCTTGTGTTTAGAGGATCTAACAAGCAAAAACACATGTTTAAAAAGAACAACAAAGCGCTTATATGGGGTAGGCCTCCCAAAGTTGTAGGAGGAAAGTTGCTTGCTTCTGGTTATTGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTACCATGTGGGATAAGCTCCCCAATTCCATACTTTTATCCCACATATCTCCTCCTTCTGCTAATATGGAGAGAAAGACGGGATGAAGCTCGATGCGCTGAGAAATATAAAGACATTTGGGCGGAGTATACCAAACTCGTCCCTTGGAGAATACTGCCTTATGTTTATTAGCATAGACAGATGTTGTTACTTGTGTGAAATATGAACACTCTCGTCGCCGAGATTTTCGTCGTCTTCTTGTCTGGTGCTTCTCTTCTCTTCTTCCTGAGGACACAACTTTCATCTGCTTCCCATGTTCATGAATTAATAGAATTGAGCAGCTGGTGACATGAACTTGACTTTGAGTCTCCGAGGCTATA

Coding sequence (CDS)

ATGATCGATTTCTTGAGGAATATGGATCTGGAGTTTCTTCTTCAACTTGCAATCCCTTCTTGGGAAGCTGTTGCTATACTTTCCATTTTCTTCTTTTACTTGGCGATTGCTGGCTCTGTTCTACCTGGAAGGATTATCCCAGGAGTGATCTTGTCGGACCGGACTAGGCTTCATTATCGCTGCAATGGTCTACTCTCTCTTCTGGTGCTTCTTGCACTTCTTGGGATTGGTGTTAAGATGAATTACATTTCGCCCACTGCAATTTCAGATAGAGGGGTGGAGCTGTTATCCGCAACCTTCTGGTTTAGTGTCCTGGCAACATTTTTACTTTGTGTTGCTGGCTACAGTTCAAGTAGTCAAAGTTCTTCTCTAAAGCCCTGTATCACAGGGAACTTTATTCACGATTGGTGGTTTGGTATACAGTTGAATCCTCACTTTTTGGGCATTGATCTCAAATTTTTCTTTGTGAGAGCTGGAATGATGGGGTGGCTACTTATTAACCTATCAGTTCTTGCCAGAAGTGTTTTGGACGCCACCCTAAGCCAATCAATGATTCTCTACCAGACATTTTGTGCGTTATATGTCCTGGACTACTTCGTTCACGAGGAGTACATGACCTCCACAAGCTTATATGTTGCTACTCTTGGATACCGGTGCTCATTGAATCTCGGTTATGTTATTCTTATTATGACTATTGTCTGTCTGAGATGGGACATAATTGCTGAAAGATTAGGCTTCATGCTGGTGTTTGGAGATCTTGTTTGGATTCCTTTTACATTTAGCATTCAGGGTTGGTGGCTTTTAACAAACAAGGCTGAGCTAACAACAGCTGCTACCATAGCTAACTGCTCGGTCTTTTTAATCGGATATCTTGTGTTTAGAGGATCTAACAAGCAAAAACACATGTTTAAAAAGAACAACAAAGCGCTTATATGGGGTAGGCCTCCCAAAGTTGTAGGAGGAAAGTTGCTTGCTTCTGGTTATTGGGGAATTGCTAGGCATTGTAATTACCTTGGAGACTTGTTGCTTGCACTTTCTTTCAGCTTACCATGTGGGATAAGCTCCCCAATTCCATACTTTTATCCCACATATCTCCTCCTTCTGCTAATATGGAGAGAAAGACGGGATGAAGCTCGATGCGCTGAGAAATATAAAGACATTTGGGCGGAGTATACCAAACTCGTCCCTTGGAGAATACTGCCTTATGTTTATTAG

Protein sequence

MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Homology
BLAST of Cp4.1LG08g10590 vs. ExPASy Swiss-Prot
Match: Q9LDR4 (Delta(14)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=FK PE=1 SV=2)

HSP 1 Score: 563.1 bits (1450), Expect = 2.5e-159
Identity = 276/400 (69.00%), Postives = 319/400 (79.75%), Query Frame = 0

Query: 5   LRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGL 64
           L +MDL  LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L YRCNGL
Sbjct: 2   LLDMDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGL 61

Query: 65  LSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSL 124
           L+L++L+A+LGI  K+  +SP  ++DRG+ELLSATF F VL T  L V G SSS++ SSL
Sbjct: 62  LALILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSL 121

Query: 125 KPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSM 184
           KP ++GN +HDWWFGIQLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV D +LSQSM
Sbjct: 122 KPHVSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSM 181

Query: 185 ILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERL 244
           ILYQ FCALY+LDYFVHEEYMTST                            WDIIAERL
Sbjct: 182 ILYQIFCALYILDYFVHEEYMTST----------------------------WDIIAERL 241

Query: 245 GFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFK 304
           GFMLVFGDL+WIPFTFSIQGWWLL NK ELT  A + NC VFLIGY+VFRG+NKQKH+FK
Sbjct: 242 GFMLVFGDLLWIPFTFSIQGWWLLHNKVELTVPAIVVNCLVFLIGYMVFRGANKQKHIFK 301

Query: 305 KNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTY 364
           KN K  IWG+PP VVGGKLL SGYWGIARHCNYLGDL+LALSFSLPCGISSP+PYFYP Y
Sbjct: 302 KNPKTPIWGKPPVVVGGKLLVSGYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIY 361

Query: 365 LLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
           LL+LLIWRERRDE RCAEKYK+IWAEY +LVPWRILPYVY
Sbjct: 362 LLILLIWRERRDEVRCAEKYKEIWAEYLRLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. ExPASy Swiss-Prot
Match: G4SW86 (Delta(14)-sterol reductase OS=Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) OX=1091494 GN=erg PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.4e-66
Identity = 149/399 (37.34%), Postives = 221/399 (55.39%), Query Frame = 0

Query: 12  FLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLL 71
           F   +A P+W A  + + +F   A      PG  + G+ L D +RL YR NG+ S L  L
Sbjct: 62  FWSHVAPPTWHAAGLYAAWFLGQAALQVWAPGPTVQGMKLPDGSRLDYRMNGIFSFLFTL 121

Query: 72  ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGN 131
           A++   V M ++  T + D+   LL+    F+ +    L   G +        +P  TG 
Sbjct: 122 AVVFGLVTMGWLDATVLYDQLGPLLTVVNIFTFVFAGFLYFWGLNGKQWE---RP--TGR 181

Query: 132 FIHDWWFGIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQT 191
             +D++ G  LNP    +DLK F   R GM+ WLL+NLS+ A+   L  T++  M+L   
Sbjct: 182 PFYDYFMGTALNPRIGSLDLKLFCEARPGMIFWLLMNLSMAAKQYELHGTVTVPMLLVVG 241

Query: 192 FCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLV 251
           F + Y++DYF+HEE + +T                            WDI  E+ G+ML 
Sbjct: 242 FQSFYLIDYFIHEEAVLTT----------------------------WDIKHEKFGWMLC 301

Query: 252 FGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKA 311
           +GDLVW+PFT+++Q  +L+ +  +L     IA  ++ L GY +FRG+N QKH F+++   
Sbjct: 302 WGDLVWLPFTYTLQAQYLVHHTHDLPVWGIIAIVALNLAGYAIFRGANIQKHHFRRDPNR 361

Query: 312 LIWGRPPKVV----GGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYL 371
           ++WG+P K +    G  LL SG+WGIARH NY GDL++ALS+ LP    SPIPYF+  Y 
Sbjct: 362 IVWGKPAKYIKTKQGSLLLTSGWWGIARHMNYFGDLMIALSWCLPAAFGSPIPYFHIVYF 421

Query: 372 LLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
            +LL+ RE+RD+A C  KY + W +Y K VPWRI+P +Y
Sbjct: 422 TILLLHREKRDDAMCLAKYGEDWLQYRKKVPWRIVPKIY 427

BLAST of Cp4.1LG08g10590 vs. ExPASy Swiss-Prot
Match: Q71KT5 (Delta(14)-sterol reductase TM7SF2 OS=Mus musculus OX=10090 GN=Tm7sf2 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 1.4e-58
Identity = 148/394 (37.56%), Postives = 222/394 (56.35%), Query Frame = 0

Query: 21  WEAVAILSIFFFY-LAIAGSVLPGR-IIPGVILSDRTRLHYRCNGLLSLLVLLALLGIGV 80
           W   A+L +F +  L +A  +LP R +  G+ L D++RL Y  NG  +L++   L+G+GV
Sbjct: 58  WSPWALLLLFIWLGLQVALYLLPARKVAEGLELKDKSRLRYPINGFQALVLTALLMGLGV 117

Query: 81  KMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSS--SQSSSLKP-CITGNFIHD 140
            +  +   A+    + L  AT     L +F+  +  Y+ +  + +S+L P   +GN ++D
Sbjct: 118 SVG-LPLGALPGMLLPLAFAT----TLTSFIFSLLLYAKALVAPASALAPGGNSGNSMYD 177

Query: 141 WWFGIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQTFCAL 200
           ++ G +LNP     D K+F  +R G++GW+ INL++L +   L  + S +M L   F  L
Sbjct: 178 FFLGRELNPRLGSFDFKYFCELRPGLIGWVFINLALLMQEAELRGSPSLAMWLVNGFQLL 237

Query: 201 YVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLVFGDL 260
           YV D   +EE + +T                             DII +  GFMLVFGDL
Sbjct: 238 YVGDALWYEESVLTT----------------------------MDIIHDGFGFMLVFGDL 297

Query: 261 VWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKN-NKALIW 320
            W+PFT+S+Q  +LL +   L     +  C + +IGY +FRG+N QK+ F+KN +   + 
Sbjct: 298 AWVPFTYSLQAQFLLYHPQPLGLPMALLICLLKVIGYYIFRGANSQKNTFRKNPSDPSVA 357

Query: 321 GRP--PKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLI 380
           G    P   G +LL SG+WG+ RH NYLGDL++AL++SLPCG+S  +PYFY  Y   LL+
Sbjct: 358 GLETIPTATGRQLLVSGWWGMVRHPNYLGDLIMALAWSLPCGLSHLLPYFYVLYFTALLV 417

Query: 381 WRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
            RE RDE +C +KY   W EY K VP+RI+PYVY
Sbjct: 418 HREARDEQQCLQKYGRAWQEYCKRVPYRIIPYVY 418

BLAST of Cp4.1LG08g10590 vs. ExPASy Swiss-Prot
Match: O08984 (Delta(14)-sterol reductase LBR OS=Rattus norvegicus OX=10116 GN=Lbr PE=1 SV=1)

HSP 1 Score: 218.4 bits (555), Expect = 1.5e-55
Identity = 143/393 (36.39%), Postives = 209/393 (53.18%), Query Frame = 0

Query: 21  WEA-VAILSIFFFYLAIAGSVLP-GRIIPGVILSDRTRLHYRCNGLLSLLVLLALLGIGV 80
           WEA V  + + +F+L    S+LP G+++ G  L D  RL YR NGL + ++  A +G  V
Sbjct: 259 WEARVCGVYLLWFFLQALFSLLPVGKVVEGTPLVDGRRLKYRLNGLYAFILTSAAVGTAV 318

Query: 81  KMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWWF 140
             + I    +    ++   A   FSV+ +  L     S       L P  +GN ++D++ 
Sbjct: 319 FWD-IELYYLYTHFLQFALAAIVFSVVLSVYLYAR--SLKVPRDELSPASSGNAVYDFFI 378

Query: 141 GIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSVL---DATLSQSMILYQTFCALY 200
           G +LNP     DLKFF  +R G++GW++INL +L   +     +  S +M L  +F  LY
Sbjct: 379 GRELNPRIGAFDLKFFCELRPGLIGWVVINLVMLLAEMKVQERSAPSLAMTLVNSFQLLY 438

Query: 201 VLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLVFGDLV 260
           V+D    EE + +T                             DII +  GFML FGDLV
Sbjct: 439 VVDALWFEEALLTT----------------------------MDIIHDGFGFMLAFGDLV 498

Query: 261 WIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKN---NKALI 320
           W+PFT+S+Q ++L+ +  +L+   T    ++ L GY++FR +N QK+ F+KN    K   
Sbjct: 499 WVPFTYSLQAFYLVNHPQDLSWPLTSVIIALKLCGYVIFRCANSQKNAFRKNPTDPKLAH 558

Query: 321 WGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLLLLIW 380
               P      LL SG+WG  RH NYLGDL++AL++SLPCG +  +PYFY  Y   LLI 
Sbjct: 559 LKTIPTSTWKSLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHILPYFYVIYFTALLIH 618

Query: 381 RERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
           RE RDE +C  KY   W +Y + VP+RI PY+Y
Sbjct: 619 REARDEHQCRRKYGLAWEKYCQRVPYRIFPYIY 620

BLAST of Cp4.1LG08g10590 vs. ExPASy Swiss-Prot
Match: Q3U9G9 (Delta(14)-sterol reductase LBR OS=Mus musculus OX=10090 GN=Lbr PE=1 SV=2)

HSP 1 Score: 216.9 bits (551), Expect = 4.3e-55
Identity = 144/403 (35.73%), Postives = 213/403 (52.85%), Query Frame = 0

Query: 21  WE--AVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLLALLGI-- 80
           WE     +  ++FF  A+   +  G++  G  L D  RL YR NGL + ++  A LG   
Sbjct: 265 WEPRVCGVYLLWFFVQALFHLLPVGKVAEGTPLVDGRRLQYRLNGLYAFILTSAALGAAV 324

Query: 81  --GVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIH 140
             GV++ Y     +    ++L  A   FSVL +  L V   S  +    L P  +GN ++
Sbjct: 325 FWGVELCY-----LYTHFLQLALAATGFSVLLSAYLYVR--SLRAPREELSPASSGNAVY 384

Query: 141 DWWFGIQLNPHFLGIDLKFFF-VRAGMMGWLLINLSVLARSVL---DATLSQSMILYQTF 200
           D++ G +LNP     DLKFF  +R G++GW++INL +L   +     A  S +MIL  +F
Sbjct: 385 DFFIGRELNPRLGAFDLKFFCELRPGLIGWVVINLVMLLMEMKIQERAAPSLAMILVNSF 444

Query: 201 CALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLVF 260
             LYV+D   +EE + ++                             DI+ +  GFML F
Sbjct: 445 QLLYVVDALWNEEALLTS----------------------------MDIMHDGFGFMLAF 504

Query: 261 GDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNNKAL 320
           GDLVW+PFT+S+Q ++L+++  +L+        ++ L GY++FR +N QK+ F+KN    
Sbjct: 505 GDLVWVPFTYSLQAFYLVSHPHDLSWPLASVIIALKLCGYVIFRCANSQKNAFRKN---- 564

Query: 321 IWGRPPKV---------VGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFY 380
                PK+          G  LL SG+WG  RH NYLGDL++AL++SLPCG +  +PYFY
Sbjct: 565 --PTDPKLAHLKTIHTSTGKSLLVSGWWGFVRHPNYLGDLIMALAWSLPCGFNHLLPYFY 624

Query: 381 PTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
             Y   LLI RE RDE +C  KY   W +Y + VP+RI PY+Y
Sbjct: 625 IIYFTALLIHREARDEHQCRRKYGLAWEKYCQRVPYRIFPYIY 626

BLAST of Cp4.1LG08g10590 vs. NCBI nr
Match: XP_023540573.1 (delta(14)-sterol reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 744 bits (1922), Expect = 1.71e-270
Identity = 376/404 (93.07%), Postives = 376/404 (93.07%), Query Frame = 0

Query: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60
           MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR
Sbjct: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60

Query: 61  CNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120
           CNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ
Sbjct: 61  CNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120

Query: 121 SSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180
           SSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL
Sbjct: 121 SSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180

Query: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDII 240
           SQSMILYQTFCALYVLDYFVHEEYMTST                            WDII
Sbjct: 181 SQSMILYQTFCALYVLDYFVHEEYMTST----------------------------WDII 240

Query: 241 AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK 300
           AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK
Sbjct: 241 AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK 300

Query: 301 HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF 360
           HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF
Sbjct: 301 HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF 360

Query: 361 YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 376

BLAST of Cp4.1LG08g10590 vs. NCBI nr
Match: XP_022947797.1 (delta(14)-sterol reductase-like [Cucurbita moschata] >KAG6596538.1 Delta(14)-sterol reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028071.1 Delta(14)-sterol reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 742 bits (1916), Expect = 1.40e-269
Identity = 374/404 (92.57%), Postives = 376/404 (93.07%), Query Frame = 0

Query: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60
           MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR
Sbjct: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60

Query: 61  CNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120
           CNGLLSLLVL+ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ
Sbjct: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120

Query: 121 SSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180
           SSSLKPCITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL
Sbjct: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180

Query: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDII 240
           SQSMILYQTFCALYVLDYFVHEEYMTST                            WDII
Sbjct: 181 SQSMILYQTFCALYVLDYFVHEEYMTST----------------------------WDII 240

Query: 241 AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK 300
           AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK
Sbjct: 241 AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK 300

Query: 301 HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF 360
           HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF
Sbjct: 301 HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF 360

Query: 361 YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 376

BLAST of Cp4.1LG08g10590 vs. NCBI nr
Match: XP_023005820.1 (delta(14)-sterol reductase-like [Cucurbita maxima])

HSP 1 Score: 718 bits (1853), Expect = 4.30e-260
Identity = 362/397 (91.18%), Postives = 366/397 (92.19%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDLEFLLQLAIPSWEAVAIL IFF YLAIAGSVLPGRIIPGVIL DRTRLHYRCNGLLSL
Sbjct: 1   MDLEFLLQLAIPSWEAVAILFIFFVYLAIAGSVLPGRIIPGVILPDRTRLHYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAG+SSSSQSSSLKPC
Sbjct: 61  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGHSSSSQSSSLKPC 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCALYVLDYFVHEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCALYVLDYFVHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTNK+ELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNKSELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL
Sbjct: 301 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. NCBI nr
Match: KAA0043924.1 (delta(14)-sterol reductase [Cucumis melo var. makuwa] >TYK25215.1 delta(14)-sterol reductase [Cucumis melo var. makuwa])

HSP 1 Score: 699 bits (1804), Expect = 1.26e-252
Identity = 350/397 (88.16%), Postives = 361/397 (90.93%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FL Q +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP 
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCA+YVLDYFVHEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCAIYVLDYFVHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC +FLIGYLVFRGSNKQKHMFKKNN
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCLIFLIGYLVFRGSNKQKHMFKKNN 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWG+PPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYP YLL+
Sbjct: 301 KALIWGKPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLI 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. NCBI nr
Match: XP_038904165.1 (delta(14)-sterol reductase isoform X1 [Benincasa hispida])

HSP 1 Score: 699 bits (1803), Expect = 1.79e-252
Identity = 346/397 (87.15%), Postives = 362/397 (91.18%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FLLQ +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLLQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQSSSLKP 
Sbjct: 61  LVLIALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPY 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           +TGNF+HDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLS+LARS+L+ATLSQSMILY
Sbjct: 121 VTGNFVHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSILARSILNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCA+YVLDYF+HEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCAIYVLDYFIHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC VF+IGYLVFRGSNKQKHMFKKNN
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCLVFVIGYLVFRGSNKQKHMFKKNN 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWG+PPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYP YLL+
Sbjct: 301 KALIWGKPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLI 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRD+ARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDDARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. ExPASy TrEMBL
Match: A0A6J1G7F4 (delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111451551 PE=3 SV=1)

HSP 1 Score: 742 bits (1916), Expect = 6.80e-270
Identity = 374/404 (92.57%), Postives = 376/404 (93.07%), Query Frame = 0

Query: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60
           MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR
Sbjct: 1   MIDFLRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYR 60

Query: 61  CNGLLSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120
           CNGLLSLLVL+ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ
Sbjct: 61  CNGLLSLLVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQ 120

Query: 121 SSSLKPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180
           SSSLKPCITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL
Sbjct: 121 SSSLKPCITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATL 180

Query: 181 SQSMILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDII 240
           SQSMILYQTFCALYVLDYFVHEEYMTST                            WDII
Sbjct: 181 SQSMILYQTFCALYVLDYFVHEEYMTST----------------------------WDII 240

Query: 241 AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK 300
           AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK
Sbjct: 241 AERLGFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQK 300

Query: 301 HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF 360
           HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF
Sbjct: 301 HMFKKNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYF 360

Query: 361 YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 YPTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 376

BLAST of Cp4.1LG08g10590 vs. ExPASy TrEMBL
Match: A0A6J1KYF4 (delta(14)-sterol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498711 PE=3 SV=1)

HSP 1 Score: 718 bits (1853), Expect = 2.08e-260
Identity = 362/397 (91.18%), Postives = 366/397 (92.19%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDLEFLLQLAIPSWEAVAIL IFF YLAIAGSVLPGRIIPGVIL DRTRLHYRCNGLLSL
Sbjct: 1   MDLEFLLQLAIPSWEAVAILFIFFVYLAIAGSVLPGRIIPGVILPDRTRLHYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAG+SSSSQSSSLKPC
Sbjct: 61  LVLVALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGHSSSSQSSSLKPC 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFG+QLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGMQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCALYVLDYFVHEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCALYVLDYFVHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTNK+ELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNKSELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL
Sbjct: 301 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. ExPASy TrEMBL
Match: A0A5A7TKR7 (Delta(14)-sterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G003450 PE=3 SV=1)

HSP 1 Score: 699 bits (1804), Expect = 6.10e-253
Identity = 350/397 (88.16%), Postives = 361/397 (90.93%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FL Q +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP 
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCA+YVLDYFVHEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCAIYVLDYFVHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC +FLIGYLVFRGSNKQKHMFKKNN
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCLIFLIGYLVFRGSNKQKHMFKKNN 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWG+PPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYP YLL+
Sbjct: 301 KALIWGKPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLI 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. ExPASy TrEMBL
Match: A0A6J1F6L0 (delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111441342 PE=3 SV=1)

HSP 1 Score: 697 bits (1799), Expect = 3.53e-252
Identity = 349/397 (87.91%), Postives = 362/397 (91.18%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL+ LLQ+ IPSWEAVAIL IFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL
Sbjct: 1   MDLDSLLQVLIPSWEAVAILFIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           L+L+ALLGIGVKMNYISPTAISDRG+ELLSATFWFSVLATFLLC+AGYSSSSQSSSLKP 
Sbjct: 61  LLLVALLGIGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCIAGYSSSSQSSSLKPR 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
            TGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ATGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCA+YV+DYFVHEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCAIYVIDYFVHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTN+AELTTAATIANC VFLIGYLVFRGSNKQKHMFKKN+
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNRAELTTAATIANCLVFLIGYLVFRGSNKQKHMFKKNS 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWG+PPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYP YLL+
Sbjct: 301 KALIWGKPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLI 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. ExPASy TrEMBL
Match: A0A1S3B6J9 (LOW QUALITY PROTEIN: delta(14)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC103486569 PE=3 SV=1)

HSP 1 Score: 696 bits (1797), Expect = 7.11e-252
Identity = 348/397 (87.66%), Postives = 360/397 (90.68%), Query Frame = 0

Query: 8   MDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSL 67
           MDL FL Q +IPSWEAVAIL IFFFYLAIAGSVLPGR+IPGVILSDRTRL YRCNGLLSL
Sbjct: 1   MDLGFLFQASIPSWEAVAILFIFFFYLAIAGSVLPGRVIPGVILSDRTRLQYRCNGLLSL 60

Query: 68  LVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPC 127
           LVL+ALLG+GVKMNYISPTAISDRG+ELLSATFWFSVLATFLLCVAGYSSSSQS+SLKP 
Sbjct: 61  LVLVALLGVGVKMNYISPTAISDRGLELLSATFWFSVLATFLLCVAGYSSSSQSASLKPH 120

Query: 128 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSMILY 187
           ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVL+ATLSQSMILY
Sbjct: 121 ITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLNATLSQSMILY 180

Query: 188 QTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFM 247
           QTFCA+YVLDYFVHEEYMTST                            WDIIAERLGFM
Sbjct: 181 QTFCAIYVLDYFVHEEYMTST----------------------------WDIIAERLGFM 240

Query: 248 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKKNN 307
           LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANC +F +GYLVFRGSNKQKHMFKKNN
Sbjct: 241 LVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCLIFXLGYLVFRGSNKQKHMFKKNN 300

Query: 308 KALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTYLLL 367
           KALIWG+PPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYP YLL+
Sbjct: 301 KALIWGKPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPMYLLI 360

Query: 368 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 404
           LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY
Sbjct: 361 LLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. TAIR 10
Match: AT3G52940.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 563.1 bits (1450), Expect = 1.7e-160
Identity = 276/400 (69.00%), Postives = 319/400 (79.75%), Query Frame = 0

Query: 5   LRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGL 64
           L +MDL  LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L YRCNGL
Sbjct: 2   LLDMDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGL 61

Query: 65  LSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSL 124
           L+L++L+A+LGI  K+  +SP  ++DRG+ELLSATF F VL T  L V G SSS++ SSL
Sbjct: 62  LALILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSL 121

Query: 125 KPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSM 184
           KP ++GN +HDWWFGIQLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV D +LSQSM
Sbjct: 122 KPHVSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSM 181

Query: 185 ILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERL 244
           ILYQ FCALY+LDYFVHEEYMTST                            WDIIAERL
Sbjct: 182 ILYQIFCALYILDYFVHEEYMTST----------------------------WDIIAERL 241

Query: 245 GFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFK 304
           GFMLVFGDL+WIPFTFSIQGWWLL NK ELT  A + NC VFLIGY+VFRG+NKQKH+FK
Sbjct: 242 GFMLVFGDLLWIPFTFSIQGWWLLHNKVELTVPAIVVNCLVFLIGYMVFRGANKQKHIFK 301

Query: 305 KNNKALIWGRPPKVVGGKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYPTY 364
           KN K  IWG+PP VVGGKLL SGYWGIARHCNYLGDL+LALSFSLPCGISSP+PYFYP Y
Sbjct: 302 KNPKTPIWGKPPVVVGGKLLVSGYWGIARHCNYLGDLMLALSFSLPCGISSPVPYFYPIY 361

Query: 365 LLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
           LL+LLIWRERRDE RCAEKYK+IWAEY +LVPWRILPYVY
Sbjct: 362 LLILLIWRERRDEVRCAEKYKEIWAEYLRLVPWRILPYVY 369

BLAST of Cp4.1LG08g10590 vs. TAIR 10
Match: AT3G52940.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 310.8 bits (795), Expect = 1.6e-84
Identity = 165/280 (58.93%), Postives = 202/280 (72.14%), Query Frame = 0

Query: 5   LRNMDLEFLLQLAIPSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGL 64
           L +MDL  LL    PS ++V +L  +F YLA+AG +LPG++I GV+LSD ++L YRCNGL
Sbjct: 2   LLDMDLGVLL----PSLQSVYVLVFYFVYLAVAGEILPGKVIRGVLLSDGSQLRYRCNGL 61

Query: 65  LSLLVLLALLGIGVKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSL 124
           L+L++L+A+LGI  K+  +SP  ++DRG+ELLSATF F VL T  L V G SSS++ SSL
Sbjct: 62  LALILLVAILGICAKLGIVSPLVVADRGLELLSATFIFCVLVTLALYVTGRSSSNKGSSL 121

Query: 125 KPCITGNFIHDWWFGIQLNPHFLGIDLKFFFVRAGMMGWLLINLSVLARSVLDATLSQSM 184
           KP ++GN +HDWWFGIQLNP F+ IDLKFFFVRAGMMGWLLINLS+LA+SV D +LSQSM
Sbjct: 122 KPHVSGNLVHDWWFGIQLNPQFMSIDLKFFFVRAGMMGWLLINLSILAKSVQDGSLSQSM 181

Query: 185 ILYQTFCALYVLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERL 244
           ILYQ FCALY+LDYFVHEEYMTST                            WDIIAERL
Sbjct: 182 ILYQIFCALYILDYFVHEEYMTST----------------------------WDIIAERL 241

Query: 245 GFMLVFGDLVWIPFTFSIQGWWLLTNKAELTTAATIANCS 285
           GFMLVFGDL+WIPFTFSIQ W  L   A+ +   +  +CS
Sbjct: 242 GFMLVFGDLLWIPFTFSIQAW--LVAFAQQSRTNSSCDCS 247

BLAST of Cp4.1LG08g10590 vs. TAIR 10
Match: AT1G50430.2 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 148.7 bits (374), Expect = 1.0e-35
Identity = 115/402 (28.61%), Postives = 190/402 (47.26%), Query Frame = 0

Query: 19  PSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLLALLGIGV 78
           P+  A  I+  +  + AI   +LPG+ + G I     R  Y+ NGL +  V LA   +G+
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATY-LGL 121

Query: 79  KMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWWF 138
                +P  + D   E+ SA  + S +   LL + G+ + S S S      GN I D+++
Sbjct: 122 WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGS---CGNLIIDFYW 181

Query: 139 GIQLNPHF-LGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQTFCALYV 198
           G++L P      D+K F   R GMM W ++ ++   +   ++  +S SM++      +YV
Sbjct: 182 GMELYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVYV 241

Query: 199 LDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLVFGDLVW 258
             +F  E    +T                             DI  +R GF + +G LVW
Sbjct: 242 TKFFWWEAGYWNT----------------------------MDIAHDRAGFYICWGCLVW 301

Query: 259 IPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKK-NNKALIWGR 318
           +P  ++  G +L+ +  EL T   I      ++   +    ++Q+  F++ N K L+WGR
Sbjct: 302 VPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCLVWGR 361

Query: 319 -PPKVVG-----------GKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFYP 378
            P K+V              LL SG+WG+ARH +Y+ ++L A  +++P    + + YFY 
Sbjct: 362 APSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFYV 421

Query: 379 TYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
            +L LLL  R +RD+ RC  KY   W  Y + V +RI+P +Y
Sbjct: 422 IFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 431

BLAST of Cp4.1LG08g10590 vs. TAIR 10
Match: AT1G50430.1 (Ergosterol biosynthesis ERG4/ERG24 family )

HSP 1 Score: 145.2 bits (365), Expect = 1.1e-34
Identity = 116/403 (28.78%), Postives = 190/403 (47.15%), Query Frame = 0

Query: 19  PSWEAVAILSIFFFYLAIAGSVLPGRIIPGVILSDRTRLHYRCNGLLSLLVLLA-LLGIG 78
           P+  A  I+  +  + AI   +LPG+ + G I     R  Y+ NGL +  V LA  LG+ 
Sbjct: 62  PTLIAWKIIFCYGAFEAILQLLLPGKRVEGPISPAGNRPVYKANGLAAYFVTLATYLGLW 121

Query: 79  VKMNYISPTAISDRGVELLSATFWFSVLATFLLCVAGYSSSSQSSSLKPCITGNFIHDWW 138
                 +P  + D   E+ SA  + S +   LL + G+ + S S S      GN I D++
Sbjct: 122 -WFGIFNPAIVYDHLGEIFSALIFGSFIFCVLLYIKGHVAPSSSDSGS---CGNLIIDFY 181

Query: 139 FGIQLNPHF-LGIDLKFFF-VRAGMMGWLLINLSVLARSV-LDATLSQSMILYQTFCALY 198
           +G++L P      D+K F   R GMM W ++ ++   +   ++  +S SM++      +Y
Sbjct: 182 WGMELYPRIGKSFDIKVFTNCRFGMMSWAVLAVTYCIKQYEINGKVSDSMLVNTILMLVY 241

Query: 199 VLDYFVHEEYMTSTSLYVATLGYRCSLNLGYVILIMTIVCLRWDIIAERLGFMLVFGDLV 258
           V  +F  E    +T                             DI  +R GF + +G LV
Sbjct: 242 VTKFFWWEAGYWNT----------------------------MDIAHDRAGFYICWGCLV 301

Query: 259 WIPFTFSIQGWWLLTNKAELTTAATIANCSVFLIGYLVFRGSNKQKHMFKK-NNKALIWG 318
           W+P  ++  G +L+ +  EL T   I      ++   +    ++Q+  F++ N K L+WG
Sbjct: 302 WVPSVYTSPGMYLVNHPVELGTQLAIYILVAGILCIYINYDCDRQRQEFRRTNGKCLVWG 361

Query: 319 R-PPKVVG-----------GKLLASGYWGIARHCNYLGDLLLALSFSLPCGISSPIPYFY 378
           R P K+V              LL SG+WG+ARH +Y+ ++L A  +++P    + + YFY
Sbjct: 362 RAPSKIVASYTTTSGETKTSLLLTSGWWGLARHFHYVPEILSAFFWTVPALFDNFLAYFY 421

Query: 379 PTYLLLLLIWRERRDEARCAEKYKDIWAEYTKLVPWRILPYVY 405
             +L LLL  R +RD+ RC  KY   W  Y + V +RI+P +Y
Sbjct: 422 VIFLTLLLFDRAKRDDDRCRSKYGKYWKLYCEKVKYRIIPGIY 432

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LDR42.5e-15969.00Delta(14)-sterol reductase OS=Arabidopsis thaliana OX=3702 GN=FK PE=1 SV=2[more]
G4SW861.4e-6637.34Delta(14)-sterol reductase OS=Methylomicrobium alcaliphilum (strain DSM 19304 / ... [more]
Q71KT51.4e-5837.56Delta(14)-sterol reductase TM7SF2 OS=Mus musculus OX=10090 GN=Tm7sf2 PE=1 SV=2[more]
O089841.5e-5536.39Delta(14)-sterol reductase LBR OS=Rattus norvegicus OX=10116 GN=Lbr PE=1 SV=1[more]
Q3U9G94.3e-5535.73Delta(14)-sterol reductase LBR OS=Mus musculus OX=10090 GN=Lbr PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023540573.11.71e-27093.07delta(14)-sterol reductase-like [Cucurbita pepo subsp. pepo][more]
XP_022947797.11.40e-26992.57delta(14)-sterol reductase-like [Cucurbita moschata] >KAG6596538.1 Delta(14)-ste... [more]
XP_023005820.14.30e-26091.18delta(14)-sterol reductase-like [Cucurbita maxima][more]
KAA0043924.11.26e-25288.16delta(14)-sterol reductase [Cucumis melo var. makuwa] >TYK25215.1 delta(14)-ster... [more]
XP_038904165.11.79e-25287.15delta(14)-sterol reductase isoform X1 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G7F46.80e-27092.57delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111451551 PE... [more]
A0A6J1KYF42.08e-26091.18delta(14)-sterol reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111498711 PE=3... [more]
A0A5A7TKR76.10e-25388.16Delta(14)-sterol reductase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A6J1F6L03.53e-25287.91delta(14)-sterol reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111441342 PE... [more]
A0A1S3B6J97.11e-25287.66LOW QUALITY PROTEIN: delta(14)-sterol reductase OS=Cucumis melo OX=3656 GN=LOC10... [more]
Match NameE-valueIdentityDescription
AT3G52940.11.7e-16069.00Ergosterol biosynthesis ERG4/ERG24 family [more]
AT3G52940.21.6e-8458.93Ergosterol biosynthesis ERG4/ERG24 family [more]
AT1G50430.21.0e-3528.61Ergosterol biosynthesis ERG4/ERG24 family [more]
AT1G50430.11.1e-3428.78Ergosterol biosynthesis ERG4/ERG24 family [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001171Ergosterol biosynthesis ERG4/ERG24PFAMPF01222ERG4_ERG24coord: 13..208
e-value: 6.0E-28
score: 97.8
coord: 233..404
e-value: 4.6E-54
score: 183.8
NoneNo IPR availableGENE3D1.20.120.1630coord: 219..403
e-value: 3.1E-20
score: 74.7
NoneNo IPR availablePANTHERPTHR21257:SF51BNACNNG50210D PROTEINcoord: 7..216
NoneNo IPR availablePANTHERPTHR21257DELTA(14)-STEROL REDUCTASEcoord: 7..216
NoneNo IPR availablePANTHERPTHR21257DELTA(14)-STEROL REDUCTASEcoord: 233..404
NoneNo IPR availablePANTHERPTHR21257:SF51BNACNNG50210D PROTEINcoord: 233..404
IPR018083Sterol reductase, conserved sitePROSITEPS01018STEROL_REDUCT_2coord: 368..391

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g10590.1Cp4.1LG08g10590.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0030176 integral component of endoplasmic reticulum membrane
cellular_component GO:0016020 membrane
molecular_function GO:0050613 delta14-sterol reductase activity
molecular_function GO:0016628 oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor