Cp4.1LG08g09190 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g09190
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionbeta-carotene isomerase D27, chloroplastic
LocationCp4.1LG08: 7227816 .. 7232383 (+)
RNA-Seq ExpressionCp4.1LG08g09190
SyntenyCp4.1LG08g09190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAAGTTCATCAAAACCTCTCGATGATCTCGAATCGATGATATTGTTCACTTCAACAGTCTCAACCGACATGATTGTAATTTCAGTTTCACCTAAAGTACCTCATTCCGTTCCCTATATCTCAATGCCTGTCCTATTCTTGTCCACGTCTCTAGAATTGTGAATGGATAGAAACGAGACGATATCAACTACCCAGAACGTGTATTGTATGCATCGTTAGGATATCATATCAATTAAAGCAAAATTTGAAGAGGAAGAAGCATATAGTACCATACCACAAATGGTGGTCTTGAAGCTACAAAGCGCCCAATTCTTCACAGCTCCGTGTGAGATTAGGAGTAGAAAAAGGGGGAATGGTCAGAAATTTAGTAGAATTATTCGATGTGGGATTGCGGAGGCTTCTGGTGAGCCAGCGCCATTGGGCCAAAAAACCAAATACAATGATGGGCCATTTGAGAAGGTTTTCATGACACTTTTTGCAAAGAAAATGGAGAAATACGCAAATTCTAAGGAGCAAACGAAGAAGATCGGATGGTGGGATTTTCTTTATGACTATGAACGATTCGTCGATGCGTCAAAAAGGGTTATGCAAGGGAAGAATCGTATGCAACAACAGCTCGTGGTTCGTGAGGTTCTCTTGTCTATGCTCCCTCCCGGCGCTCCGGCTCAGGTCGGCCTGTTTTTCTTTCTGTAAATTTTCGTCTTCTCCGATCAACGTGTGAACTGAATCGGAAACTGATGATCGCAGTTCAGGAAATTGTTTCCTCCAACGAAATGGGCTTGTGAGTTCAATGCTTCGATAACGGTGCCGTTCTTTCAGTGGTTAGTCGGCCCATCGGAGGTAATTTCATTGAATCCTTTGTGTTCTGTTTCACTTTCTTGGCTCTGTTTTCTATTGATTTGAACGTTTGTTTGAGTTCTTCCATTGGGGAATTATATGCTCTGACTCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTGCTCTGAATCTGTAGTGTTGATGATCGGTAGTTTATGTGCTATGAACTGCTAGGGTTAGTGAGTGATAATTGCTTATGTGCTGCATTCTAGGTGGGTTTGTTGTCTGTTGTTTTAGAATCAAGAGTTTTGCCCTGAACTGCTTGGTTCTACTAGCTGCATTAGGGATAGTAGTTGGACAAGCTCATGGGTTTTGATGGTACCCTAAGTTATTCTCCGAACTCGTTGTGAGATCCCACATTGGTTGGAGAGGGGAACGAAACATTCCTAGTAAGGGCGTGGAAACTTCTCCTTAGTAGACTTGTTTTAAAATATTGAGGGGAAGCCTAAAAGAGAAAGACTAAAGAGGACAGTATAGCAGTGGGCTTAAGATGTTACAAATGGTATTAGAGCCGGACATTGGGCAGTGTGACAACGAGGACGTTGAGCCTCTAAAGGGGTGGATTGTGAGATCCCACATTAGTTGGAGAGAGGAATGAAACATTCTTTACATGGGTGTGAAAACCTCTCCCTTGGAGACGCATTCTAAAACTTTGAGGGGAAGCTCAGAATGGAAAGCCTGAATAAGACAATTTCTACTAGCAGTGGGCTTGGACGGTTGCAAATGGTATCAGAGCTAGATACTGGGCGGTGTGCCGACGAGAACGTTGAGCCTCCAAATGGGGTGAATTGTGAGATCCCACATTGGTTTGGGGAGGGGAACAAAGGATTTCTTATAAGGGTGTGGAAACCTCTCCCTAGCAGATGTGTTCTAAAACCTTGAGGGGAAGCTAAGAAAAGCCCAAAGAGAACAATATCGGCTAGCGGTGGGCTTGGGTTGTTACAAATGGTATCAGAGTCAGACGCCAGGCTGTGTGACAACGAAAACGCTGAGACCCCAAGGGGAGTGGAGCCGTCACACGAAAACGCTGAGACCCCAAGGGGAGTGGAGTCGTCACACGAAAACGCTGAGACCCCAAGGGGAGTGGAGCCCAAAAGGGAAAGCCCAAAGAGGACAACATCTGTTAGCAGTGGACTTGGGTTGCTACAAATGGTATCAGAGTCAAACACCGAGCGGTGTGACAATGAGGACGCTGATCCCCCAAGAGGAGTGGAGCCCAGGAGGGAAAGTTCAAAGAGGACACATCTACTAGCGGTGGACTTGGGTTGTTACAAATGGTATCAAACAAGACAAATGTGTAAGGTGCAAATGCACGAGGCTTTCTAGTCTTCTTAAGTGTTGAGAGCTTGGTTTAAAAGGTTAAAAATCTATTTTGAGATCGGCTTCTAGCATCTAGCAGGGAGAGGTTTCCACACTCTTGGAAAGAATGTTTCGTTCCCCTCTCCAACCGATGTCGGATCCACAATCCACCTCCCTTGGGACCCAACGTCTTCGCTAGCACAACGCTCGGTGCCTGACATTGATACCATTTGTAACAATCCAAGCCCAGCATGAGTCCTCTTTGGACTTTCCCTTCCGGGCTTCCCTCAAGGTTTTAAAACGCGTCTACTAGGGAGAGGTTTCTACACCCTTATAAGGAATGTTTCGTTCCCCTCTCCAACCAATGTAGGATCTCACGATCCACCCCTTTCGGGGTTCCAGCGTCCTCGCTGGCATACTGCTCGATGCCTAGCTCTGATACCATTTGTAACAGCCCAAGCCCACTGCTAGCAGATATTGTCCTCTTTAGGCTTTCCTTTCTAAGGTTCCCCTCAAAGTTTTAAAACGTGTCTTACTAGGAAGAGGTTTCCACACCTTATAAAGAATATTTTGTTCCCCTCTCCAACCAATATGGGATCTCACAGTAACTCATTTGCTTAAACTTTTGAGTTCGATCGTTGCATTACAGTGTTATAACGTGTCTTACTAGGAAGAGGTTTCCACACCTTATAAAGAATATTTTGTTCCCCTCTCCAACCAATATGGGATCTCACAGTAACTCATTTGCTTAAACTTTTGAGTTCGATCGTTGCATTACAGTGTTATTGAAGCTTTAATATTAGAGTACGACGAAACAACTAGAAATTCGATTTTCAGTGATTAAACTCAGTAGGTTTCTATGGTACTTGAAAAGAACAGTATATTTCAGCTGTTGGTTTACTCAACCACAGCTCCTTGAGTCTCTGAGGGTTCAAGGATGTTAACTGATCCAATGGCGTCGATAATGGGTTACGATGCAGTTGGCTTGAATCTGTGTTTTCGGACCGTGCAACAACTGCCTAATCCTACCATATTTAGTTCGAAACTCACGAAATTATGATCTTGTTCTTCCTCGTGTGTAGGTTGTGGAAGTGGAGGTAAACGGTATAAAGCAAAGAAGCGGAGTTCATATAAAGAAATGCAGGTATAAACTACATTTTCGTTATAACCAAATTTCTAGTCGATTAAGGAAAACACTCCTCCTTCATCTGTCTCCGTCGATAAGCTAAGACTATGACATGAACGAACGCGAATCAGTCCATTCGATTTTCTTTAACGACTTTTGAATCCCGATCAAAGGTACCTCGAGAACAGTGGGTGTGTGGGTATGTGTGTGAATATGTGCAAGATACCCACACAAGATTTCTTCACCAACGAATTTGGGCTCCCTCTCACCATGAATCCGAGTATGTATACTGTTTTACAAGAACATATATATACATATATATGCATTTTCAGTATTGTTTTGACTGATCAGATCAGATTCAAGGCCTAAACCATACTTTGTTCTTGCTTGATCACCTTGTTAGATTTTGAAGACATGAGCTGTGAGATGATATACGGGCAAGTTCCGCCGCCGTTCGAAGAGGATCCCGTGTCGAAGCAACCGTGCTACAAGGATATATGTAAGAGGAATCTGTTAGTTCTTGCAATGATCAAGTATGTTCTTGGTATTTAGCTGATTTGTGTTCTTCATCTGTGTTTGTTTGCTGACAGGTTCCATGTCCAATCCTAGCTCCTCGTTATGTCCTAAATTACTAGCTTAAATGACATTCTTTCATGGTTTGATTCTGTTCATCACCATGTCTGCACACGTTGTCGGATACATACTGTAGTGTATAGATTCTCTTGCTATGTCAGAGAGGCAACATTGTTGTTGTTTCTTTGCAAACTTTACTATGTTTGCTAGATCATATACAATAAAGAAGATTATTTCCTTAGGAATGTTTCATGAACATTCGGTCGGGATTTGAGCATCTGACTTACTTTTTTGGTCGAGAATTCAATGTCTTAACCAATTGAAAGTTCACGATACGAGGTAAATCTTGTTACGATTTGTTA

mRNA sequence

CAAGTTCATCAAAACCTCTCGATGATCTCGAATCGATGATATTGTTCACTTCAACAGTCTCAACCGACATGATTGTAATTTCAGTTTCACCTAAAGTACCTCATTCCGTTCCCTATATCTCAATGCCTGTCCTATTCTTGTCCACGTCTCTAGAATTGTGAATGGATAGAAACGAGACGATATCAACTACCCAGAACGTGTATTGTATGCATCGTTAGGATATCATATCAATTAAAGCAAAATTTGAAGAGGAAGAAGCATATAGTACCATACCACAAATGGTGGTCTTGAAGCTACAAAGCGCCCAATTCTTCACAGCTCCGTGTGAGATTAGGAGTAGAAAAAGGGGGAATGGTCAGAAATTTAGTAGAATTATTCGATGTGGGATTGCGGAGGCTTCTGGTGAGCCAGCGCCATTGGGCCAAAAAACCAAATACAATGATGGGCCATTTGAGAAGGTTTTCATGACACTTTTTGCAAAGAAAATGGAGAAATACGCAAATTCTAAGGAGCAAACGAAGAAGATCGGATGGTGGGATTTTCTTTATGACTATGAACGATTCGTCGATGCGTCAAAAAGGGTTATGCAAGGGAAGAATCGTATGCAACAACAGCTCGTGGTTCGTGAGGTTCTCTTGTCTATGCTCCCTCCCGGCGCTCCGGCTCAGTTCAGGAAATTGTTTCCTCCAACGAAATGGGCTTGTGAGTTCAATGCTTCGATAACGGTGCCGTTCTTTCAGTGGTTAGTCGGCCCATCGGAGGTTGTGGAAGTGGAGGTAAACGGTATAAAGCAAAGAAGCGGAGTTCATATAAAGAAATGCAGGTACCTCGAGAACAGTGGGTGTGTGGGTATGTGTGTGAATATGTGCAAGATACCCACACAAGATTTCTTCACCAACGAATTTGGGCTCCCTCTCACCATGAATCCGAATTTTGAAGACATGAGCTGTGAGATGATATACGGGCAAGTTCCGCCGCCGTTCGAAGAGGATCCCGTGTCGAAGCAACCGTGCTACAAGGATATATGTTCCATGTCCAATCCTAGCTCCTCGTTATGTCCTAAATTACTAGCTTAAATGACATTCTTTCATGGTTTGATTCTGTTCATCACCATGTCTGCACACGTTGTCGGATACATACTGTAGTGTATAGATTCTCTTGCTATGTCAGAGAGGCAACATTGTTGTTGTTTCTTTGCAAACTTTACTATGTTTGCTAGATCATATACAATAAAGAAGATTATTTCCTTAGGAATGTTTCATGAACATTCGGTCGGGATTTGAGCATCTGACTTACTTTTTTGGTCGAGAATTCAATGTCTTAACCAATTGAAAGTTCACGATACGAGGTAAATCTTGTTACGATTTGTTA

Coding sequence (CDS)

ATGGTGGTCTTGAAGCTACAAAGCGCCCAATTCTTCACAGCTCCGTGTGAGATTAGGAGTAGAAAAAGGGGGAATGGTCAGAAATTTAGTAGAATTATTCGATGTGGGATTGCGGAGGCTTCTGGTGAGCCAGCGCCATTGGGCCAAAAAACCAAATACAATGATGGGCCATTTGAGAAGGTTTTCATGACACTTTTTGCAAAGAAAATGGAGAAATACGCAAATTCTAAGGAGCAAACGAAGAAGATCGGATGGTGGGATTTTCTTTATGACTATGAACGATTCGTCGATGCGTCAAAAAGGGTTATGCAAGGGAAGAATCGTATGCAACAACAGCTCGTGGTTCGTGAGGTTCTCTTGTCTATGCTCCCTCCCGGCGCTCCGGCTCAGTTCAGGAAATTGTTTCCTCCAACGAAATGGGCTTGTGAGTTCAATGCTTCGATAACGGTGCCGTTCTTTCAGTGGTTAGTCGGCCCATCGGAGGTTGTGGAAGTGGAGGTAAACGGTATAAAGCAAAGAAGCGGAGTTCATATAAAGAAATGCAGGTACCTCGAGAACAGTGGGTGTGTGGGTATGTGTGTGAATATGTGCAAGATACCCACACAAGATTTCTTCACCAACGAATTTGGGCTCCCTCTCACCATGAATCCGAATTTTGAAGACATGAGCTGTGAGATGATATACGGGCAAGTTCCGCCGCCGTTCGAAGAGGATCCCGTGTCGAAGCAACCGTGCTACAAGGATATATGTTCCATGTCCAATCCTAGCTCCTCGTTATGTCCTAAATTACTAGCTTAA

Protein sequence

MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYKDICSMSNPSSSLCPKLLA
Homology
BLAST of Cp4.1LG08g09190 vs. ExPASy Swiss-Prot
Match: R4HZ96 (Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27 PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.3e-41
Identity = 86/208 (41.35%), Postives = 126/208 (60.58%), Query Frame = 0

Query: 53  YNDGPFEKVFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQ 112
           Y D  F+K+ +   +K ++  A +     K G       ++  V+A+    Q  N  QQQ
Sbjct: 53  YKDNWFDKLAINHLSKSVQ--AATGISNNKSG-------FDSLVEAATVASQKFNTTQQQ 112

Query: 113 LVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQ 172
            ++ + L    P    +  R++ PP+K A E+ A  T  FF WL+GPSEV E E+NG ++
Sbjct: 113 GIILDALDRAFPKPILSVIRRVMPPSKLAREYFAVFTTIFFAWLLGPSEVRESEINGRRE 172

Query: 173 RSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVP 232
           ++ VHIKKCR+LE + CVGMC+N+CK+P+Q F  + FG+P+ M PNF+DMSCEMI+GQ P
Sbjct: 173 KNIVHIKKCRFLEETNCVGMCINLCKMPSQLFIKDSFGMPVNMVPNFDDMSCEMIFGQEP 232

Query: 233 PPFEEDPVSKQPCYKDICSMSNPSSSLC 261
           P   +DP  KQPCYK +C      ++ C
Sbjct: 233 PASTDDPALKQPCYK-LCKAKKNHATQC 250

BLAST of Cp4.1LG08g09190 vs. ExPASy Swiss-Prot
Match: Q7XA78 (Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D27 PE=1 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 9.8e-40
Identity = 87/222 (39.19%), Postives = 125/222 (56.31%), Query Frame = 0

Query: 30  SRIIRCGIAEASGEPAPLGQKTKYN----DGPFEKVFMTLFAKKMEKYANSKEQTKKIGW 89
           S+ +  G  +A+ E A +      N    D  F K+ +   +K ++  A     +K    
Sbjct: 38  SKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKST-- 97

Query: 90  WDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPTKWACEFN 149
                DY+R VD + RV +  +  QQ   V   L   LP    +  +  FPP+K + E  
Sbjct: 98  -----DYDRLVDTATRVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELF 157

Query: 150 ASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFF 209
           A  T   F WLVGPSEV E EVNG K++S V+I+KCR+LE S CVGMC ++CKIP+Q F 
Sbjct: 158 ALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFI 217

Query: 210 TNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYK 248
            N  G+P+ M P+F D+SC+M++G+ PP  E+DP  KQPC++
Sbjct: 218 KNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFE 252

BLAST of Cp4.1LG08g09190 vs. ExPASy Swiss-Prot
Match: C7AU21 (Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=D27 PE=1 SV=1)

HSP 1 Score: 161.4 bits (407), Expect = 1.4e-38
Identity = 80/208 (38.46%), Postives = 123/208 (59.13%), Query Frame = 0

Query: 43  EPAPLGQKTKYNDGPFEKVFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRV 102
           E A     T Y D  F+K+ +   ++ +++ +  K +            YE  +DA+  +
Sbjct: 70  ETAAAPPATVYRDSWFDKLAIGYLSRNLQEASGLKNEKD---------GYESLIDAALAI 129

Query: 103 MQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEV 162
            +  +  +Q  +V + L   LP       + + PP++++ E+ A+ T  FF WLVGP EV
Sbjct: 130 SRIFSLDKQSEIVTQALERALPSYILTMIKVMMPPSRFSREYFAAFTTIFFPWLVGPCEV 189

Query: 163 VEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDM 222
           +E EV G K+++ V+I KCR+LE++ CVGMC N+CKIP Q F  +  G+ + M+PNFEDM
Sbjct: 190 MESEVEGRKEKNVVYIPKCRFLESTNCVGMCTNLCKIPCQKFIQDSLGMKVYMSPNFEDM 249

Query: 223 SCEMIYGQVPPPFEEDPVSKQPCYKDIC 251
           SCEMI+GQ PP  E+DP  KQPC++  C
Sbjct: 250 SCEMIFGQQPP--EDDPALKQPCFRTKC 266

BLAST of Cp4.1LG08g09190 vs. NCBI nr
Match: XP_023540699.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 556 bits (1434), Expect = 2.41e-200
Identity = 265/265 (100.00%), Postives = 265/265 (100.00%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60
           MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK
Sbjct: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60

Query: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120
           VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL
Sbjct: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120

Query: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180
           SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK
Sbjct: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180

Query: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240
           CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV
Sbjct: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240

Query: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265
           SKQPCYKDICSMSNPSSSLCPKLLA
Sbjct: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Cp4.1LG08g09190 vs. NCBI nr
Match: XP_022942554.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita moschata])

HSP 1 Score: 552 bits (1422), Expect = 1.63e-198
Identity = 262/265 (98.87%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60
           MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK
Sbjct: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60

Query: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120
           VFMTLFAKKME+YANSKEQ KKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL
Sbjct: 61  VFMTLFAKKMERYANSKEQMKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120

Query: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180
           SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNG+KQRSGVHIKK
Sbjct: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGMKQRSGVHIKK 180

Query: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240
           CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV
Sbjct: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240

Query: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265
           SKQPCYKDICSMSNPSSSLCPKLLA
Sbjct: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Cp4.1LG08g09190 vs. NCBI nr
Match: XP_023005623.1 (beta-carotene isomerase D27, chloroplastic [Cucurbita maxima])

HSP 1 Score: 551 bits (1421), Expect = 2.31e-198
Identity = 263/265 (99.25%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60
           MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK
Sbjct: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60

Query: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120
           VFMTLFAKKME+YANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL
Sbjct: 61  VFMTLFAKKMERYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120

Query: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180
           SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK
Sbjct: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180

Query: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240
           CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP FEEDPV
Sbjct: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPLFEEDPV 240

Query: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265
           SKQPCYKDICSMSNPSSSLCPKLLA
Sbjct: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Cp4.1LG08g09190 vs. NCBI nr
Match: KAG6596672.1 (Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 550 bits (1417), Expect = 9.42e-198
Identity = 261/265 (98.49%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60
           MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK
Sbjct: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60

Query: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120
           VFMTLFAKKME+YANSKEQ KKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL
Sbjct: 61  VFMTLFAKKMERYANSKEQMKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120

Query: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180
           SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK
Sbjct: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180

Query: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240
           CRYLENSGCVGMCVNMCK+PTQDFFT+EFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV
Sbjct: 181 CRYLENSGCVGMCVNMCKMPTQDFFTSEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240

Query: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265
           SKQPCYKDICSMSNPSSSLCPKLLA
Sbjct: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Cp4.1LG08g09190 vs. NCBI nr
Match: XP_008448557.1 (PREDICTED: beta-carotene isomerase D27, chloroplastic [Cucumis melo] >KAA0052966.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa] >TYK11422.1 beta-carotene isomerase D27 [Cucumis melo var. makuwa])

HSP 1 Score: 496 bits (1276), Expect = 2.75e-176
Identity = 244/267 (91.39%), Postives = 250/267 (93.63%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPC-EIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTAP  EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKISK----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKYANSKEQTKKI-GWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREV 120
           KVFMTLFA+KMEK+AN+KEQ KK  G+WDFLYDYERFVD SKRVMQGKNRMQQQLVVREV
Sbjct: 61  KVFMTLFARKMEKFANAKEQRKKKEGFWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREV 120

Query: 121 LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI 180
           LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI
Sbjct: 121 LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI 180

Query: 181 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 240
           KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED
Sbjct: 181 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 240

Query: 241 PVSKQPCYKDICSMSNPSSSLCPKLLA 265
           PVSKQPCYKDICSMSN S+ LCPKLLA
Sbjct: 241 PVSKQPCYKDICSMSNTSAPLCPKLLA 263

BLAST of Cp4.1LG08g09190 vs. ExPASy TrEMBL
Match: A0A6J1FRN3 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111447551 PE=4 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 7.88e-199
Identity = 262/265 (98.87%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60
           MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK
Sbjct: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60

Query: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120
           VFMTLFAKKME+YANSKEQ KKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL
Sbjct: 61  VFMTLFAKKMERYANSKEQMKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120

Query: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180
           SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNG+KQRSGVHIKK
Sbjct: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGMKQRSGVHIKK 180

Query: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240
           CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV
Sbjct: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240

Query: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265
           SKQPCYKDICSMSNPSSSLCPKLLA
Sbjct: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Cp4.1LG08g09190 vs. ExPASy TrEMBL
Match: A0A6J1L2P3 (beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111498565 PE=4 SV=1)

HSP 1 Score: 551 bits (1421), Expect = 1.12e-198
Identity = 263/265 (99.25%), Postives = 264/265 (99.62%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60
           MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK
Sbjct: 1   MVVLKLQSAQFFTAPCEIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFEK 60

Query: 61  VFMTLFAKKMEKYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120
           VFMTLFAKKME+YANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL
Sbjct: 61  VFMTLFAKKMERYANSKEQTKKIGWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLL 120

Query: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180
           SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK
Sbjct: 121 SMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKK 180

Query: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPV 240
           CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPP FEEDPV
Sbjct: 181 CRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPLFEEDPV 240

Query: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265
           SKQPCYKDICSMSNPSSSLCPKLLA
Sbjct: 241 SKQPCYKDICSMSNPSSSLCPKLLA 265

BLAST of Cp4.1LG08g09190 vs. ExPASy TrEMBL
Match: A0A5A7UFH7 (Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00760 PE=4 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 1.33e-176
Identity = 244/267 (91.39%), Postives = 250/267 (93.63%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPC-EIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTAP  EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKISK----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKYANSKEQTKKI-GWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREV 120
           KVFMTLFA+KMEK+AN+KEQ KK  G+WDFLYDYERFVD SKRVMQGKNRMQQQLVVREV
Sbjct: 61  KVFMTLFARKMEKFANAKEQRKKKEGFWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREV 120

Query: 121 LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI 180
           LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI
Sbjct: 121 LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI 180

Query: 181 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 240
           KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED
Sbjct: 181 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 240

Query: 241 PVSKQPCYKDICSMSNPSSSLCPKLLA 265
           PVSKQPCYKDICSMSN S+ LCPKLLA
Sbjct: 241 PVSKQPCYKDICSMSNTSAPLCPKLLA 263

BLAST of Cp4.1LG08g09190 vs. ExPASy TrEMBL
Match: A0A1S3BKU4 (beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103490700 PE=4 SV=1)

HSP 1 Score: 496 bits (1276), Expect = 1.33e-176
Identity = 244/267 (91.39%), Postives = 250/267 (93.63%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPC-EIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTAP  EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKISK----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKYANSKEQTKKI-GWWDFLYDYERFVDASKRVMQGKNRMQQQLVVREV 120
           KVFMTLFA+KMEK+AN+KEQ KK  G+WDFLYDYERFVD SKRVMQGKNRMQQQLVVREV
Sbjct: 61  KVFMTLFARKMEKFANAKEQRKKKEGFWDFLYDYERFVDVSKRVMQGKNRMQQQLVVREV 120

Query: 121 LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI 180
           LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI
Sbjct: 121 LLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHI 180

Query: 181 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 240
           KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED
Sbjct: 181 KKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEED 240

Query: 241 PVSKQPCYKDICSMSNPSSSLCPKLLA 265
           PVSKQPCYKDICSMSN S+ LCPKLLA
Sbjct: 241 PVSKQPCYKDICSMSNTSAPLCPKLLA 263

BLAST of Cp4.1LG08g09190 vs. ExPASy TrEMBL
Match: A0A0A0L682 (DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002850 PE=4 SV=1)

HSP 1 Score: 485 bits (1248), Expect = 2.46e-172
Identity = 239/268 (89.18%), Postives = 246/268 (91.79%), Query Frame = 0

Query: 1   MVVLKLQSAQFFTAPC-EIRSRKRGNGQKFSRIIRCGIAEASGEPAPLGQKTKYNDGPFE 60
           MVVLKLQS QFFTAP  EIR+RK       SR IRCGIAEASGEPAPLGQKTKYNDGPFE
Sbjct: 1   MVVLKLQSIQFFTAPPKEIRNRKIK-----SRFIRCGIAEASGEPAPLGQKTKYNDGPFE 60

Query: 61  KVFMTLFAKKMEKYANSKEQTKKIG--WWDFLYDYERFVDASKRVMQGKNRMQQQLVVRE 120
           KVFMTLFA+KMEK+AN+KEQ KK    WWDFLYDYERFVD SKRVMQGK RMQQQ+VVRE
Sbjct: 61  KVFMTLFARKMEKFANAKEQRKKKEGLWWDFLYDYERFVDVSKRVMQGKTRMQQQIVVRE 120

Query: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNASITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180
           VLLSMLPPGAPAQFRKLFPPTKWACEFNA ITVPFFQWLVGPSEVVEVEVNGIKQRSGVH
Sbjct: 121 VLLSMLPPGAPAQFRKLFPPTKWACEFNALITVPFFQWLVGPSEVVEVEVNGIKQRSGVH 180

Query: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240
           IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE
Sbjct: 181 IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEE 240

Query: 241 DPVSKQPCYKDICSMSNPSSSLCPKLLA 265
           DPVSKQPCYKDICSM+  S+ LCPKLLA
Sbjct: 241 DPVSKQPCYKDICSMAITSAPLCPKLLA 263

BLAST of Cp4.1LG08g09190 vs. TAIR 10
Match: AT1G64680.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G03055.1); Has 146 Blast hits to 146 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 125; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 387.1 bits (993), Expect = 1.1e-107
Identity = 182/236 (77.12%), Postives = 206/236 (87.29%), Query Frame = 0

Query: 31  RIIRCGIAEASGEPAPLGQKTKYNDGPFEKVFMTLFAKKMEKYANSKEQ-TKKIGWWDFL 90
           R  RCGIAE SGEPAP+G KT+Y DG  E+VFM LFA+KM+K+ + K++ TK+ G+W+  
Sbjct: 17  RSTRCGIAEPSGEPAPMGLKTRYEDGLVERVFMGLFARKMDKFGSKKKKDTKEKGFWE-- 76

Query: 91  YDYERFVDASKRVMQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPTKWACEFNASIT 150
           YDYE FV+ SKRVMQG++R+QQQ  VREVLLSMLPPGAP QFRKLFPPTKWA EFNA++T
Sbjct: 77  YDYESFVEVSKRVMQGRSRVQQQEAVREVLLSMLPPGAPEQFRKLFPPTKWAAEFNAALT 136

Query: 151 VPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEF 210
           VPFF WLVGPS+V+EVEVNG+KQRSGV IKKCRYLENSGCVGMCVNMCKIPTQDFFTNEF
Sbjct: 137 VPFFHWLVGPSQVIEVEVNGVKQRSGVRIKKCRYLENSGCVGMCVNMCKIPTQDFFTNEF 196

Query: 211 GLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYKDICSMSNPSSSLCPKLLA 266
           GLPLTMNPN+EDMSCEMIYGQ PP FEED  +KQPC  DICSMSNPSS +CPKL A
Sbjct: 197 GLPLTMNPNYEDMSCEMIYGQAPPAFEEDVATKQPCLADICSMSNPSSPICPKLEA 250

BLAST of Cp4.1LG08g09190 vs. TAIR 10
Match: AT1G03055.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 143 Blast hits to 143 proteins in 26 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 0; Plants - 122; Viruses - 0; Other Eukaryotes - 15 (source: NCBI BLink). )

HSP 1 Score: 165.2 bits (417), Expect = 7.0e-41
Identity = 87/222 (39.19%), Postives = 125/222 (56.31%), Query Frame = 0

Query: 30  SRIIRCGIAEASGEPAPLGQKTKYN----DGPFEKVFMTLFAKKMEKYANSKEQTKKIGW 89
           S+ +  G  +A+ E A +      N    D  F K+ +   +K ++  A     +K    
Sbjct: 38  SKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKST-- 97

Query: 90  WDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPTKWACEFN 149
                DY+R VD + RV +  +  QQ   V   L   LP    +  +  FPP+K + E  
Sbjct: 98  -----DYDRLVDTATRVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELF 157

Query: 150 ASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQDFF 209
           A  T   F WLVGPSEV E EVNG K++S V+I+KCR+LE S CVGMC ++CKIP+Q F 
Sbjct: 158 ALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKCRFLEQSNCVGMCTHICKIPSQIFI 217

Query: 210 TNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYK 248
            N  G+P+ M P+F D+SC+M++G+ PP  E+DP  KQPC++
Sbjct: 218 KNSLGMPIYMEPDFNDLSCKMMFGREPPEIEDDPAMKQPCFE 252

BLAST of Cp4.1LG08g09190 vs. TAIR 10
Match: AT4G01995.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 132.9 bits (333), Expect = 3.8e-31
Identity = 82/241 (34.02%), Postives = 128/241 (53.11%), Query Frame = 0

Query: 30  SRIIRCGIAEASGEPAPLG-QKTKYNDGPFEKVFMTLFAKKM-EKYANSKEQTKKIGWWD 89
           +R +RC I+ +S   +  G  K +Y  GP +  FM  F  K+ E+  +  E+   +G  +
Sbjct: 28  TRRLRCRISNSSEVKSDEGAPKLEYKPGPLDDFFMQSFRNKLVEEVGSDSEKPGYVGLIE 87

Query: 90  FLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPT---KWACEF 149
            +            +++G+ R +       +L S+ PP     ++ L  P    K A   
Sbjct: 88  LV---------KLLLLKGRTRSETSDAAVRILKSLFPPLILELYKLLIAPIAQGKLAALM 147

Query: 150 NASITVPFFQWLVGPSEVVEVEV-NGIKQRSGVHIKKCRYLENSGCVGMCVNMCKIPTQD 209
            A +TV   QWL+GPS+V  +++ NG    SGV ++KC+YLE S CVG+C+N CK+PTQ 
Sbjct: 148 VARVTVLTCQWLMGPSKVNIIDLPNGESWDSGVFVEKCQYLEESKCVGVCINTCKLPTQT 207

Query: 210 FFTNEFGLPLTMNPNFEDMSCEMIYGQVPPPFEEDPVSKQPCYKDICSMSNP---SSSLC 262
           FF +  G+PL M PNF+D SC+  +G  PP  E+D    +PC+ + CS++      S  C
Sbjct: 208 FFKDYMGVPLVMEPNFKDYSCQFKFGVAPP--EDDGNVNEPCF-ETCSIAGRRKLKSGEC 256

BLAST of Cp4.1LG08g09190 vs. TAIR 10
Match: AT1G03055.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64680.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 79.0 bits (193), Expect = 6.6e-15
Identity = 53/157 (33.76%), Postives = 76/157 (48.41%), Query Frame = 0

Query: 30  SRIIRCGIAEASGEPAPLGQKTKYN----DGPFEKVFMTLFAKKMEKYANSKEQTKKIGW 89
           S+ +  G  +A+ E A +      N    D  F K+ +   +K ++  A     +K    
Sbjct: 38  SKPVYSGKLKAAKETARIETSNTKNASIEDSFFSKIAINYLSKNLQDAAGISSSSKST-- 97

Query: 90  WDFLYDYERFVDASKRVMQGKNRMQQQLVVREVLLSMLPPGAPAQFRKLFPPTKWACEFN 149
                DY+R VD + RV +  +  QQ   V   L   LP    +  +  FPP+K + E  
Sbjct: 98  -----DYDRLVDTATRVSRNFDTKQQHEFVLSSLDRALPTVISSLIKMAFPPSKVSRELF 157

Query: 150 ASITVPFFQWLVGPSEVVEVEVNGIKQRSGVHIKKCR 183
           A  T   F WLVGPSEV E EVNG K++S V+I+KCR
Sbjct: 158 ALFTTISFAWLVGPSEVRETEVNGRKEKSVVYIEKCR 187

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
R4HZ962.3e-4141.35Beta-carotene isomerase D27, chloroplastic OS=Medicago truncatula OX=3880 GN=D27... [more]
Q7XA789.8e-4039.19Beta-carotene isomerase D27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=D2... [more]
C7AU211.4e-3838.46Beta-carotene isomerase D27, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
Match NameE-valueIdentityDescription
XP_023540699.12.41e-200100.00beta-carotene isomerase D27, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022942554.11.63e-19898.87beta-carotene isomerase D27, chloroplastic [Cucurbita moschata][more]
XP_023005623.12.31e-19899.25beta-carotene isomerase D27, chloroplastic [Cucurbita maxima][more]
KAG6596672.19.42e-19898.49Beta-carotene isomerase D27, chloroplastic, partial [Cucurbita argyrosperma subs... [more]
XP_008448557.12.75e-17691.39PREDICTED: beta-carotene isomerase D27, chloroplastic [Cucumis melo] >KAA0052966... [more]
Match NameE-valueIdentityDescription
A0A6J1FRN37.88e-19998.87beta-carotene isomerase D27, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1L2P31.12e-19899.25beta-carotene isomerase D27, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A5A7UFH71.33e-17691.39Beta-carotene isomerase D27 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3BKU41.33e-17691.39beta-carotene isomerase D27, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1034907... [more]
A0A0A0L6822.46e-17289.18DUF4033 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002850 PE=... [more]
Match NameE-valueIdentityDescription
AT1G64680.11.1e-10777.12unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G03055.17.0e-4139.19unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G01995.13.8e-3134.02unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT1G03055.26.6e-1533.76unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025114Beta-carotene isomerase D27-like, C-terminalPFAMPF13225DUF4033coord: 155..237
e-value: 1.1E-38
score: 131.1
IPR038938Beta-carotene isomerase D27-likePANTHERPTHR33591BETA-CAROTENE ISOMERASE D27coord: 22..263
NoneNo IPR availablePANTHERPTHR33591:SF4OS08G0114100 PROTEINcoord: 22..263

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g09190.1Cp4.1LG08g09190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016853 isomerase activity