Cp4.1LG08g08260 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g08260
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionRNA-binding protein pno1
LocationCp4.1LG08: 6549615 .. 6552601 (+)
RNA-Seq ExpressionCp4.1LG08g08260
SyntenyCp4.1LG08g08260
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CCGTTGGGCAAGATCCCGCCCCTATATATCTTCCTCTTCATTCCACACCTTCTTTCAACATCCGCCATTACCAATCCTTCTTCCTGCAAAAGCTCAAAGAATTTCATCAATTCTTTCTCAAATTTAATTCATCAAATTCTCCATCTCCGATTAAAAATGGGTTTCTCCATAAGCGGCCACCCACAATCATCAACCTCAATGAGCCGTTCCCATTACCACACCCACAAGCTCTTCCTCTACACCAATTACATCCTCCTCGGCGCCGCCTCCAGCTGCATTTTCCTCACTCTTTCCCTCCGCCTCCTCCCCTCTCTCTGCGGTCTCTCCCTCATCTTCCTCCACATCCTCACCATCGCCGCCGCCGTCTCCGGCTGCGCCATGGCCTCCACCGCCTCGGGTCGTTGGTTCGGCATCCACATGGTGTTCACCGTCCTCACCGCCATCTTCCAAGGCTCTGTTGCTGTCCTGGTGTACACCAGAACAGGGGACTTTCTTGGGGAGTTGAAATCGTACGTGAGGGAGGAGGATGGAGCCGTGATCTTGAAATTGGCCGGAGGGTTGAGTGTGGTTATGTTTGTTTTGGAGTGGGTTGTTCTTACTTTGGCGTTTTTCTTGAAGTATTATGCGTTTGTTGAAGGCGGTGGAGGGAGTGGGAATGGGGCGGCGGCGATGAGGAGTGCTAAGGTTGAACAGGATGAGGATTTGAAGGACTGGCCTTGGCCGTTCCAAGTTTGATTGATTCCACTTTGTTTGGATTTTATATATTTTTTGTGAAATATGTTCAATTTGTTTCTTAAAATTTAGATATGTAATCTTCAATTTATTGTTTAATTCCTTTCGGGTGTGAATCGTGTCTCTCTTTTAAAACAAAAAACATATTTTAAAGGGGTTTGGGTGAAAGTCAAGGTAATCTTATTGGCATGAAATACACAAGCCTGAAACCCCGATTTGGTGAGGTTTTTGGTATCTCAGTGCAATACTCGAGTATAATTTGGTAAGATTTTAGCAATTTTATGTCTAGTTTAGTTTTTTGTGGATGAGTCACCCATATTGTCCACTTTGAGCCTAATCTCTCTCAAGCCTATGCTGTTTTGGGTTTTTCAGAAGGCCTCATCCAATGGAGATACTATTTCTCACTTGTAAACGTGATATTTCACTAAATTATAACCAACGTTGAACTCACTCCCAATAATCCATAACAATTGTCACTCCAAATTAGTGTACTTTTTCACCTAATCAATTCTTTCGAAGTCTTTCCTTGTCCGAAAATCTTCTCTCTCCTATTCCACTCGGTCGAGTGATCCCAGACCAATTCAGTCACATTTCATTCATTTGGAATATGGGCTTTTATTAATTCAATTAGAGAATCGGAGGTCGAGTCCAAATTTTGGTGGAGTGAAATATTTGATTCTTTTTCTAATCTTCCCAGCTCTTCTTCTATACATTATTTTTTAAAAAAAAAAATTGTTTATTTATTATTTTTTTATTCCATTCGGGTAAGAGAGAAGCTTATTTAAACAACAAATCTTTTAAAGTCTATTAAAAAATATATTTTTTAGTGAAAAAATAGACACAAAAATAAAATAAAAATAATGGGCCCCAACGGCCCAATGGCCCAATGGCCCAATCCCATTTGATTTTAATTTAAGAAACAAAGATGAAAACAAAATAGAGCGAAGAAAGGATCGTTAAACCCTAAGAGCCGACTCATTTCTTCGCACTTTCTCCTCTTCCTCTTAGCAGTTAACTTAGCACCTTCCCGCCGTCTCCTCCGTCGTTCTCTGTGCCACCGTCGACGCCGTACCCCTTCTTCACATATTTGTCTACCTTTCTTGATCTCAGGTATTCTCCCTATTTACCCCCTATCTTTCTCTTTCAAGTTTCATTCCGTTGCAGTGGATGACATCCCAATGGGAACTGATTTGAATTTCTGTTTTGCCGAAGTTTCATTGGTTTGGTTGCTCATTCGTACGATCCGAGGCCGACGCATTCTATTCTCGTGTTACCTAAAATTGATATTGGATTATAGTATTTTAGAATCCGGCACTGTTTGTAGTTGAATCCTACTTGCTTGTTATTAGTTAATTGTTATATTGGGTTGTAGTGTTAGAATCAATGCATCTTTTACATGAGAAAAGGAATTTTTGTTTCTGCAATTTCTCTTCTCGGGAGGGTAAGAATGAAGTTTATCAGATTTATTTTCAATTTTTGTTGCTTATCATGTTGATTGATGGGGTTTTGTTTATATTTGATCTATTACTTCGTCGTTTAACACTAGTTCATTGGCTGTTTACAGTGTGGAGACTCAGAACCATTTCATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCAACTCAACCGAAACCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCCCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAGATTTACACCCCAATTTATGAGCAGATGAAAATTGACCTCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCATTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACTTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAA

mRNA sequence

CCGTTGGGCAAGATCCCGCCCCTATATATCTTCCTCTTCATTCCACACCTTCTTTCAACATCCGCCATTACCAATCCTTCTTCCTGCAAAAGCTCAAAGAATTTCATCAATTCTTTCTCAAATTTAATTCATCAAATTCTCCATCTCCGATTAAAAATGGGTTTCTCCATAAGCGGCCACCCACAATCATCAACCTCAATGAGCCGTTCCCATTACCACACCCACAAGCTCTTCCTCTACACCAATTACATCCTCCTCGGCGCCGCCTCCAGCTGCATTTTCCTCACTCTTTCCCTCCGCCTCCTCCCCTCTCTCTGCGGTCTCTCCCTCATCTTCCTCCACATCCTCACCATCGCCGCCGCCGTCTCCGGCTGCGCCATGGCCTCCACCGCCTCGGGTCGTTGGTTCGGCATCCACATGGTGTTCACCGTCCTCACCGCCATCTTCCAAGGCTCTGTTGCTGTCCTGGTGTACACCAGAACAGGGGACTTTCTTGGGGAGTTGAAATCGTACGTGAGGGAGGAGGATGGAGCCGTGATCTTGAAATTGGCCGGAGGGTTGAGTGTGGTTATGTTTGTTTTGGAGTGGGTTGTTCTTACTTTGGCGTTTTTCTTGAAGTATTATGCGTTTGTTGAAGGCGGTGGAGGGAGTGGGAATGGGGCGGCGGCGATGAGGAGTGCTAAGGTTGAACAGGATGAGGATTTGAAGGACTGGCCTTGGCCGTTCCAAACTCAGAACCATTTCATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCAACTCAACCGAAACCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCCCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAGATTTACACCCCAATTTATGAGCAGATGAAAATTGACCTCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCATTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACTTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAA

Coding sequence (CDS)

CCGTTGGGCAAGATCCCGCCCCTATATATCTTCCTCTTCATTCCACACCTTCTTTCAACATCCGCCATTACCAATCCTTCTTCCTGCAAAAGCTCAAAGAATTTCATCAATTCTTTCTCAAATTTAATTCATCAAATTCTCCATCTCCGATTAAAAATGGGTTTCTCCATAAGCGGCCACCCACAATCATCAACCTCAATGAGCCGTTCCCATTACCACACCCACAAGCTCTTCCTCTACACCAATTACATCCTCCTCGGCGCCGCCTCCAGCTGCATTTTCCTCACTCTTTCCCTCCGCCTCCTCCCCTCTCTCTGCGGTCTCTCCCTCATCTTCCTCCACATCCTCACCATCGCCGCCGCCGTCTCCGGCTGCGCCATGGCCTCCACCGCCTCGGGTCGTTGGTTCGGCATCCACATGGTGTTCACCGTCCTCACCGCCATCTTCCAAGGCTCTGTTGCTGTCCTGGTGTACACCAGAACAGGGGACTTTCTTGGGGAGTTGAAATCGTACGTGAGGGAGGAGGATGGAGCCGTGATCTTGAAATTGGCCGGAGGGTTGAGTGTGGTTATGTTTGTTTTGGAGTGGGTTGTTCTTACTTTGGCGTTTTTCTTGAAGTATTATGCGTTTGTTGAAGGCGGTGGAGGGAGTGGGAATGGGGCGGCGGCGATGAGGAGTGCTAAGGTTGAACAGGATGAGGATTTGAAGGACTGGCCTTGGCCGTTCCAAACTCAGAACCATTTCATAATGCAGTCAACCGAAGCCTCCACTTCCATGGAAGTAGAAGCAGTTTCAACTCAACCGAAACCAGTAGTTAGCACATTACCCCCAAAACCTCACTTTGATCCTCTAAAGCCCCATGAGATGAATGATGGTCGAGTTCAGTTCCGAAAGGTCTCTGTTCCTCCGCATCGATATTCTCCGCTCAAGAAAGCATGGATGGAGATTTACACCCCAATTTATGAGCAGATGAAAATTGACCTCCGAATGAATCTCAAGGCACGAAAAGTCGAACTTAAAACCAGAGCAGACACGCCTGATATCAGTAACTTACAGAAATGTGCAGATTTTGTCCATGCTTTCATGCTCGGTTTTGATGTGATCGATGCCATTGCACTCCTACGGGTGGATGAACTCTATGTGGAGTCTTTTGAGATTAAAGATGTTAAGACGCTTCGAGGCGAGCACTTGTCTCGTGCCATAGGCAGATTGTCTGGTAAAGCTGGTAAGACAAAGTTTGCCATCGAAAACGCCACAAAGACGAGGATAGTTATTGCAGACACCAAGATTCACATATTAGGATCCTTCGCAAATATCAAGATTGCAAGGGATTCTCTTTGCAGCCTCATTTTAGGGTCCCCTGCTGGAAAAGTTTATTCAAAACTGAGAGCAGTCACAGCCAGATTGGCTGAA

Protein sequence

PLGKIPPLYIFLFIPHLLSTSAITNPSSCKSSKNFINSFSNLIHQILHLRLKMGFSISGHPQSSTSMSRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIFLHILTIAAAVSGCAMASTASGRWFGIHMVFTVLTAIFQGSVAVLVYTRTGDFLGELKSYVREEDGAVILKLAGGLSVVMFVLEWVVLTLAFFLKYYAFVEGGGGSGNGAAAMRSAKVEQDEDLKDWPWPFQTQNHFIMQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Homology
BLAST of Cp4.1LG08g08260 vs. ExPASy Swiss-Prot
Match: Q9VR89 (RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.1e-65
Identity = 121/186 (65.05%), Postives = 155/186 (83.33%), Query Frame = 0

Query: 286 PHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADT 345
           P +    R + RKVSVPPHRYS LK+ WM+I+TP+ E MK+ +R N+KAR+VEL+   +T
Sbjct: 53  PPKAKRARSELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPET 112

Query: 346 PDISNLQKCADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLS 405
           PDI+NLQ+ ADFV AF+ GF+V DA+ALLR+++L+VESFEIKDVKTLRG+H SRAIGRL+
Sbjct: 113 PDIANLQRGADFVRAFLCGFEVDDALALLRLEDLFVESFEIKDVKTLRGDHQSRAIGRLA 172

Query: 406 GKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAV 465
           GK G+TKF IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LRAV
Sbjct: 173 GKGGRTKFTIENVTKTRIVLADSKIHILGSYQNIQLARRAVCNLILGSPPSKVYGNLRAV 232

Query: 466 TARLAE 472
            +RL+E
Sbjct: 233 ASRLSE 238

BLAST of Cp4.1LG08g08260 vs. ExPASy Swiss-Prot
Match: Q29IG6 (RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l(1)G0004 PE=3 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 5.9e-64
Identity = 118/177 (66.67%), Postives = 152/177 (85.88%), Query Frame = 0

Query: 295 QFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKC 354
           + RKVSVPPHRYS LK+ WM+I+TP+ E MK+ +R N+KAR+VEL+   +TPDI+NLQK 
Sbjct: 60  ELRKVSVPPHRYSSLKEHWMKIFTPVVEHMKLQIRFNMKARQVELRVGPETPDIANLQKG 119

Query: 355 ADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFA 414
           ADFV AF+ GF+V DA+ALLR+++L+VE+FEIKDVKTLRG+H SRAIGRL+GK G+TKF 
Sbjct: 120 ADFVKAFLCGFEVDDALALLRLEDLFVETFEIKDVKTLRGDHQSRAIGRLAGKGGRTKFT 179

Query: 415 IENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 472
           IEN TKTRIV+AD+KIHILGS+ NI++AR ++C+LILGSP  KVY  LR+V +RL+E
Sbjct: 180 IENVTKTRIVLADSKIHILGSYQNIQLARRAICNLILGSPPSKVYGNLRSVASRLSE 236

BLAST of Cp4.1LG08g08260 vs. ExPASy Swiss-Prot
Match: A7RP64 (RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 3.0e-63
Identity = 116/194 (59.79%), Postives = 155/194 (79.90%), Query Frame = 0

Query: 278 KPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDLRMNLKARKV 337
           +P F P+K  ++  G+ + RK+ VP HRY+PLK+ WM+I+TP+ E +K+ +R NL +R V
Sbjct: 43  RPSFPPVKAQKLAGGKSETRKIPVPSHRYTPLKENWMKIFTPVVEHLKLQIRFNLGSRHV 102

Query: 338 ELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 397
           E++   +T DI  +QK ADFV AF+LGF+V DA+AL+R+D+L++ESFEI DVK L+G+HL
Sbjct: 103 EIRASKETSDIGAVQKAADFVQAFILGFEVEDALALIRLDDLFLESFEIADVKPLKGDHL 162

Query: 398 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 457
           SRAIGR++GK GKTKF IEN TKTRIV+A+TKIHILGSF NIKIAR ++C+LILGSP  K
Sbjct: 163 SRAIGRVAGKGGKTKFTIENVTKTRIVLAETKIHILGSFQNIKIARTAICNLILGSPPSK 222

Query: 458 VYSKLRAVTARLAE 472
           VY  +RAV +R AE
Sbjct: 223 VYGNMRAVASRSAE 236

BLAST of Cp4.1LG08g08260 vs. ExPASy Swiss-Prot
Match: Q6VEU3 (RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2)

HSP 1 Score: 242.3 bits (617), Expect = 1.1e-62
Identity = 122/209 (58.37%), Postives = 159/209 (76.08%), Query Frame = 0

Query: 264 VSTQPKPVVSTLPPK-PHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYE 323
           + TQ +   S+ P K PHF  L   ++  G  + RKV VP HRY+PLK+ W++I+TPI E
Sbjct: 42  MDTQTEESSSSAPVKRPHFPALSGDQLGGGVDEMRKVPVPSHRYTPLKENWLKIFTPIVE 101

Query: 324 QMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDELYVE 383
            +++ +R NLK R+VE+KT  +T DI  L + ADFV AF+LGF V DA+AL+R+DEL++E
Sbjct: 102 NLQLQVRFNLKTRQVEIKTCKETQDIGALTRAADFVKAFVLGFQVEDALALIRLDELFLE 161

Query: 384 SFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIA 443
           +F++ DVK L+G+HLSRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+A
Sbjct: 162 TFDVTDVKPLKGDHLSRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMA 221

Query: 444 RDSLCSLILGSPAGKVYSKLRAVTARLAE 472
           R ++C+LILGSP  KVY  LRAV  R AE
Sbjct: 222 RTAICNLILGSPPSKVYGNLRAVATRSAE 250

BLAST of Cp4.1LG08g08260 vs. ExPASy Swiss-Prot
Match: Q6VBQ6 (RNA-binding protein PNO1 OS=Oryzias latipes OX=8090 GN=pno1 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.1e-62
Identity = 117/194 (60.31%), Postives = 155/194 (79.90%), Query Frame = 0

Query: 278 KPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDLRMNLKARKV 337
           +PHF P+   ++  G  + RKV+VP HRY+PLK+ W++I+TPI E +++ +R NLK R V
Sbjct: 49  RPHFPPISADKLR-GPDEMRKVAVPAHRYTPLKENWLKIFTPIVENLQLQVRFNLKTRNV 108

Query: 338 ELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHL 397
           E+KT  +T DI+ L K ADF+ AF+LGF V DA+AL+R+DEL++ESF++ DVK L+G+HL
Sbjct: 109 EIKTCKETQDIAALTKAADFIKAFVLGFQVDDAMALIRLDELFLESFDVTDVKPLKGDHL 168

Query: 398 SRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGK 457
           SRAIGR++GK GKTKF IEN TKTRIV+ADTKIHILGSF NIK+AR ++C+LILGSP  K
Sbjct: 169 SRAIGRIAGKGGKTKFTIENVTKTRIVLADTKIHILGSFQNIKMARTAICNLILGSPPSK 228

Query: 458 VYSKLRAVTARLAE 472
           VY  +RAV +R AE
Sbjct: 229 VYGNIRAVASRTAE 241

BLAST of Cp4.1LG08g08260 vs. NCBI nr
Match: KAE8650098.1 (hypothetical protein Csa_010636 [Cucumis sativus])

HSP 1 Score: 725 bits (1872), Expect = 7.09e-261
Identity = 383/430 (89.07%), Postives = 398/430 (92.56%), Query Frame = 0

Query: 53  MGFSISGHPQSSTSMSRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIF 112
           MGFSI+  P  STSM+RSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIF
Sbjct: 1   MGFSITSTP--STSMTRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIF 60

Query: 113 LHILTIAAAVSGCAMASTASGRWFGIHMVFTVLTAIFQGSVAVLVYTRTGDFLGELKSYV 172
           LHILTIAAAVSGCAMA+ +S RWFG+HMVFTVLTAIFQGSVA+LVYTRTGDFL ELKSYV
Sbjct: 61  LHILTIAAAVSGCAMAAASSTRWFGVHMVFTVLTAIFQGSVAMLVYTRTGDFLSELKSYV 120

Query: 173 REEDGAVILKLAGGLSVVMFVLEWVVLTLAFFLKYYAFVEGGGGSGNGAAAMRSAKVEQD 232
           REEDGAVILKLAGGLSVVMFVLEWVVLTLAFFL+YYAFVE G  S N  AAMRSAKV+QD
Sbjct: 121 REEDGAVILKLAGGLSVVMFVLEWVVLTLAFFLRYYAFVEEG--SNNNGAAMRSAKVQQD 180

Query: 233 EDLKDWPWPFQTQ-----------NHFIMQSTEASTSMEVEAVSTQPKPVVSTLPPKPHF 292
           EDLKDWPWPFQ +           N+ IMQS EASTSM+VE +STQ K VVSTLPPKP F
Sbjct: 181 EDLKDWPWPFQLKILGVFSVETPHNYSIMQSNEASTSMDVETISTQTKDVVSTLPPKPQF 240

Query: 293 DPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYTPIYEQMKIDLRMNLKARKVELKT 352
           DPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWM+IYTPIY+QMKID+RMNLKARKVELKT
Sbjct: 241 DPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMDIYTPIYDQMKIDIRMNLKARKVELKT 300

Query: 353 RADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAI 412
           RADTPDISNLQK ADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAI
Sbjct: 301 RADTPDISNLQKSADFVHAFMLGFDVIDAIALLRVDELYVESFEIKDVKTLRGEHLSRAI 360

Query: 413 GRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSK 471
           GRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSK
Sbjct: 361 GRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFANIKIARDSLCSLILGSPAGKVYSK 420

BLAST of Cp4.1LG08g08260 vs. NCBI nr
Match: XP_023540523.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540524.1 RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 434 bits (1117), Expect = 2.62e-149
Identity = 222/222 (100.00%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. NCBI nr
Match: XP_023005535.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita maxima] >XP_023005536.1 RNA-binding protein pno1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 434 bits (1115), Expect = 5.27e-149
Identity = 221/222 (99.55%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. NCBI nr
Match: XP_022951370.1 (RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >XP_022951376.1 RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >KAG7028303.1 RNA-binding protein pno1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 432 bits (1110), Expect = 3.03e-148
Identity = 219/222 (98.65%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. NCBI nr
Match: XP_008449001.1 (PREDICTED: RNA-binding protein pno1 [Cucumis melo])

HSP 1 Score: 421 bits (1082), Expect = 5.38e-144
Identity = 215/222 (96.85%), Postives = 218/222 (98.20%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVE +STQ K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIY+QMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. ExPASy TrEMBL
Match: A0A6J1KXN9 (RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498497 PE=3 SV=1)

HSP 1 Score: 434 bits (1115), Expect = 2.55e-149
Identity = 221/222 (99.55%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. ExPASy TrEMBL
Match: A0A6J1GHF0 (RNA-binding protein pno1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454213 PE=3 SV=1)

HSP 1 Score: 432 bits (1110), Expect = 1.46e-148
Identity = 219/222 (98.65%), Postives = 222/222 (100.00%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVEAVS+QPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVEAVSSQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           A+ALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AVALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. ExPASy TrEMBL
Match: A0A1S3BLP2 (RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1)

HSP 1 Score: 421 bits (1082), Expect = 2.61e-144
Identity = 215/222 (96.85%), Postives = 218/222 (98.20%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVE +STQ K VVSTLPPKP FDPLKPHEMNDGRVQFRKVSVPPHRYSPL
Sbjct: 1   MQSTEASTSMEVETISTQTKDVVSTLPPKPQFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIY+QMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYDQMKIDVRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. ExPASy TrEMBL
Match: A0A6J1EWL7 (RNA-binding protein pno1 OS=Cucurbita moschata OX=3662 GN=LOC111438772 PE=3 SV=1)

HSP 1 Score: 420 bits (1079), Expect = 7.43e-144
Identity = 214/222 (96.40%), Postives = 218/222 (98.20%), Query Frame = 0

Query: 250 MQSTEASTSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPL 309
           MQSTEASTSMEVE VST+ K VVSTLPPKP FDPLKPHEMNDGR+QFRKVSVPPHRY+PL
Sbjct: 1   MQSTEASTSMEVETVSTETKNVVSTLPPKPQFDPLKPHEMNDGRIQFRKVSVPPHRYTPL 60

Query: 310 KKAWMEIYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 369
           KKAWMEIYTPIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID
Sbjct: 61  KKAWMEIYTPIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVID 120

Query: 370 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 429
           AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK
Sbjct: 121 AIALLRVDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTK 180

Query: 430 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IHILGSFANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 222

BLAST of Cp4.1LG08g08260 vs. ExPASy TrEMBL
Match: A0A6J1KTE6 (RNA-binding protein pno1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498497 PE=3 SV=1)

HSP 1 Score: 418 bits (1075), Expect = 2.18e-143
Identity = 212/213 (99.53%), Postives = 213/213 (100.00%), Query Frame = 0

Query: 259 MEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 318
           MEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT
Sbjct: 1   MEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWMEIYT 60

Query: 319 PIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDE 378
           PIYEQMKID+RMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDE
Sbjct: 61  PIYEQMKIDIRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLRVDE 120

Query: 379 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 438
           LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN
Sbjct: 121 LYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGSFAN 180

Query: 439 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 471
           IKIARDSLCSLILGSPAGKVYSKLRAVTARLAE
Sbjct: 181 IKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 213

BLAST of Cp4.1LG08g08260 vs. TAIR 10
Match: AT3G13230.1 (RNA-binding KH domain-containing protein )

HSP 1 Score: 345.9 bits (886), Expect = 5.1e-95
Identity = 174/216 (80.56%), Postives = 197/216 (91.20%), Query Frame = 0

Query: 256 STSMEVEAVSTQPKPVVSTLPPKPHFDPLKPHEMNDGRVQFRKVSVPPHRYSPLKKAWME 315
           ST MEVE  +    P    LPPKP F PLK HEM+DG+VQFRK++VPP+RYSPLKKAW++
Sbjct: 4   STQMEVETATEGTVP----LPPKPTFKPLKAHEMSDGKVQFRKIAVPPNRYSPLKKAWLD 63

Query: 316 IYTPIYEQMKIDLRMNLKARKVELKTRADTPDISNLQKCADFVHAFMLGFDVIDAIALLR 375
           IYTPIY+QMK+D+RMNLKARKVELKTRADTPDISNLQK ADFVHAFMLGFD+ DAI+LLR
Sbjct: 64  IYTPIYDQMKVDIRMNLKARKVELKTRADTPDISNLQKSADFVHAFMLGFDIPDAISLLR 123

Query: 376 VDELYVESFEIKDVKTLRGEHLSRAIGRLSGKAGKTKFAIENATKTRIVIADTKIHILGS 435
           +DELYVESFEIKDVKTL+GEHLSRAIGRLSGK GKTKFAIEN+TKTRIVIADT+IHILG+
Sbjct: 124 MDELYVESFEIKDVKTLKGEHLSRAIGRLSGKGGKTKFAIENSTKTRIVIADTRIHILGA 183

Query: 436 FANIKIARDSLCSLILGSPAGKVYSKLRAVTARLAE 472
           F+NIK+AR SLCSLI+GSPAGKVYSKLR+V+ARL E
Sbjct: 184 FSNIKVARSSLCSLIMGSPAGKVYSKLRSVSARLNE 215

BLAST of Cp4.1LG08g08260 vs. TAIR 10
Match: AT5G16250.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G02640.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 243.4 bits (620), Expect = 3.6e-64
Identity = 124/185 (67.03%), Postives = 146/185 (78.92%), Query Frame = 0

Query: 59  GHPQSSTSMSRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIFLHILTI 118
           G   SS+ +  SHYHTHK+FL++NYILLGAASSCIFLTLSLRL+PS+CG  LI LH  TI
Sbjct: 2   GFISSSSPVEESHYHTHKIFLFSNYILLGAASSCIFLTLSLRLIPSICGFLLILLHATTI 61

Query: 119 AAAVSGCAMASTASGRWFGIHMVFTVLTAIFQGSVAVLVYTRTGDFLGELKSYVREEDGA 178
           AAAVSGCA AS    RW+  HMV TVLTAIFQGSV+VL++T T  FLG LKSYVREED A
Sbjct: 62  AAAVSGCAAASCGRNRWYAAHMVATVLTAIFQGSVSVLIFTNTSKFLGSLKSYVREEDAA 121

Query: 179 VILKLAGGLSVVMFVLEWVVLTLAFFLKYYAFVEGGGGSGNGAAAMRSAKVEQDEDLKDW 238
           VILKL GGL +V+F L+W+VL  AFFLKYYA+V+G    G+G A  R+ KV+ +E+ KDW
Sbjct: 122 VILKLGGGLCIVIFCLDWIVLVCAFFLKYYAYVDG----GDGVAMKRTGKVQSEENPKDW 181

Query: 239 PWPFQ 244
           PWPFQ
Sbjct: 182 PWPFQ 182

BLAST of Cp4.1LG08g08260 vs. TAIR 10
Match: AT3G02640.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G16250.1); Has 96 Blast hits to 96 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 95; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 226.9 bits (577), Expect = 3.5e-59
Identity = 118/183 (64.48%), Postives = 141/183 (77.05%), Query Frame = 0

Query: 61  PQSSTSMSRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIFLHILTIAA 120
           PQ   S+  SHY+THKLFL  NY+LLGA+SSCIFLTLSLRL+PSLCG  LI LH  TIAA
Sbjct: 5   PQPQESIQESHYYTHKLFLTANYVLLGASSSCIFLTLSLRLIPSLCGFFLILLHATTIAA 64

Query: 121 AVSGCAMASTASGRWFGIHMVFTVLTAIFQGSVAVLVYTRTGDFLGELKSYVREEDGAVI 180
           AVSGCA AS    RW+  HM+ TVLTAIFQGSV+VL++T T +FL  L SYVRE++ ++I
Sbjct: 65  AVSGCAAASYGKNRWYAAHMIATVLTAIFQGSVSVLIFTNTSNFLESLNSYVREKEASMI 124

Query: 181 LKLAGGLSVVMFVLEWVVLTLAFFLKYYAFVEGGGGSGNGAAAMRSAKVEQDEDLKDWPW 240
           LKLAGGL VV+F LEW+VL LAFFLKYYA+V+   G  NG A  R+ KV+ +E LK+ PW
Sbjct: 125 LKLAGGLCVVIFCLEWIVLVLAFFLKYYAYVD---GDNNGVAMKRTGKVQSEETLKNSPW 184

Query: 241 PFQ 244
            FQ
Sbjct: 185 AFQ 184

BLAST of Cp4.1LG08g08260 vs. TAIR 10
Match: AT5G36800.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G36710.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 197.2 bits (500), Expect = 2.9e-50
Identity = 115/183 (62.84%), Postives = 139/183 (75.96%), Query Frame = 0

Query: 67  MSRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIFLHILTIAAAVSGCA 126
           MS+S  +TH +FL  NYILLG+ASSCIFLT+SLRL PSL GLSLIFL+ LTIA AVSGC+
Sbjct: 3   MSKSKGNTHNIFLLCNYILLGSASSCIFLTISLRLFPSLSGLSLIFLYTLTIATAVSGCS 62

Query: 127 M-----ASTASGRWFGIHMVFTVLTAIFQGSVAVLVYTRTGDFLGELKSYVREEDGAVIL 186
           +     ++TAS R +G HMV TVLTAIFQG+V+VL++TRTGDFL  LKSYVREEDG VIL
Sbjct: 63  IFASSTSATASDRLYGSHMVATVLTAIFQGAVSVLIFTRTGDFLRFLKSYVREEDGEVIL 122

Query: 187 KLAGGLSVVMFVLEWVVLTLAFFLKYYAFVEGGGGSGNGAAAMRSAKVEQDEDLKDWP-W 244
           KL+GGL V+MF LEW+VL LAF LKY  +++      +     R     Q+EDLKDWP +
Sbjct: 123 KLSGGLCVLMFCLEWIVLVLAFLLKYSDYLDESVVDDDDFKVRR-----QEEDLKDWPSY 180

BLAST of Cp4.1LG08g08260 vs. TAIR 10
Match: AT5G36710.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G36800.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 197.2 bits (500), Expect = 2.9e-50
Identity = 115/183 (62.84%), Postives = 139/183 (75.96%), Query Frame = 0

Query: 67  MSRSHYHTHKLFLYTNYILLGAASSCIFLTLSLRLLPSLCGLSLIFLHILTIAAAVSGCA 126
           MS+S  +TH +FL  NYILLG+ASSCIFLT+SLRL PSL GLSLIFL+ LTIA AVSGC+
Sbjct: 3   MSKSKGNTHNIFLLCNYILLGSASSCIFLTISLRLFPSLSGLSLIFLYTLTIATAVSGCS 62

Query: 127 M-----ASTASGRWFGIHMVFTVLTAIFQGSVAVLVYTRTGDFLGELKSYVREEDGAVIL 186
           +     ++TAS R +G HMV TVLTAIFQG+V+VL++TRTGDFL  LKSYVREEDG VIL
Sbjct: 63  IFASSTSATASDRLYGSHMVATVLTAIFQGAVSVLIFTRTGDFLRFLKSYVREEDGEVIL 122

Query: 187 KLAGGLSVVMFVLEWVVLTLAFFLKYYAFVEGGGGSGNGAAAMRSAKVEQDEDLKDWP-W 244
           KL+GGL V+MF LEW+VL LAF LKY  +++      +     R     Q+EDLKDWP +
Sbjct: 123 KLSGGLCVLMFCLEWIVLVLAFLLKYSDYLDESVVDDDDFKVRR-----QEEDLKDWPSY 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9VR894.1e-6565.05RNA-binding protein pno1 OS=Drosophila melanogaster OX=7227 GN=l(1)G0004 PE=2 SV... [more]
Q29IG65.9e-6466.67RNA-binding protein pno1 OS=Drosophila pseudoobscura pseudoobscura OX=46245 GN=l... [more]
A7RP643.0e-6359.79RNA-binding protein pno1 OS=Nematostella vectensis OX=45351 GN=pno1 PE=3 SV=1[more]
Q6VEU31.1e-6258.37RNA-binding protein PNO1 OS=Danio rerio OX=7955 GN=pno1 PE=2 SV=2[more]
Q6VBQ61.1e-6260.31RNA-binding protein PNO1 OS=Oryzias latipes OX=8090 GN=pno1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
KAE8650098.17.09e-26189.07hypothetical protein Csa_010636 [Cucumis sativus][more]
XP_023540523.12.62e-149100.00RNA-binding protein pno1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023540... [more]
XP_023005535.15.27e-14999.55RNA-binding protein pno1-like isoform X1 [Cucurbita maxima] >XP_023005536.1 RNA-... [more]
XP_022951370.13.03e-14898.65RNA-binding protein pno1-like isoform X1 [Cucurbita moschata] >XP_022951376.1 RN... [more]
XP_008449001.15.38e-14496.85PREDICTED: RNA-binding protein pno1 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1KXN92.55e-14999.55RNA-binding protein pno1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
A0A6J1GHF01.46e-14898.65RNA-binding protein pno1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A1S3BLP22.61e-14496.85RNA-binding protein pno1 OS=Cucumis melo OX=3656 GN=LOC103491005 PE=3 SV=1[more]
A0A6J1EWL77.43e-14496.40RNA-binding protein pno1 OS=Cucurbita moschata OX=3662 GN=LOC111438772 PE=3 SV=1[more]
A0A6J1KTE62.18e-14399.53RNA-binding protein pno1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11149... [more]
Match NameE-valueIdentityDescription
AT3G13230.15.1e-9580.56RNA-binding KH domain-containing protein [more]
AT5G16250.13.6e-6467.03unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G02640.13.5e-5964.48unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G36800.12.9e-5062.84unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin... [more]
AT5G36710.12.9e-5062.84unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004087K Homology domainSMARTSM00322kh_6coord: 378..451
e-value: 1.7E-4
score: 31.0
IPR036612K Homology domain, type 1 superfamilyGENE3D3.30.1370.10K Homology domain, type 1coord: 377..471
e-value: 7.8E-22
score: 79.2
IPR036612K Homology domain, type 1 superfamilySUPERFAMILY54791Eukaryotic type KH-domain (KH-domain type I)coord: 378..469
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..287
NoneNo IPR availablePANTHERPTHR12826RIBONUCLEASE Ycoord: 258..471
NoneNo IPR availableCDDcd00105KH-Icoord: 398..444
e-value: 0.0034291
score: 34.0728
IPR039912Pre-rRNA-processing protein PNO1-likePANTHERPTHR12826:SF13RNA-BINDING PROTEIN PNO1coord: 258..471

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g08260.1Cp4.1LG08g08260.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005730 nucleolus
molecular_function GO:0003723 RNA binding
molecular_function GO:0003676 nucleic acid binding