Cp4.1LG08g07700 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG08g07700
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RNA polymerase II transcription factor B subunit 5
Location	Cp4.1LG08: 6165556 .. 6168603 (-)
RNA-Seq Expression	Cp4.1LG08g07700
Synteny	Cp4.1LG08g07700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGAATGAGAATTATGGTGGGAGTGGTGGCTGTCATGGCGGCGGCGGCGGCGGCGGCGGCGACGGTGGTTTTGTTGGTAACTTCAAAATCATCTCAATTTTTTCTAAGTACTAAGCTTCTTGGGAGTCCTCATTTCATGTTCTCAATCTTCAACTCTATAATTCTTCTCATAATTGGGACATACCATCGGCCACCGCCTCATCAATCTCCCTATCATATACGAAACGGGTCGTGCTACCCAGGGTCGTATCAAGGGTACTATACTCCTTATCAACACATTAAATTATGTGTTCGTAACGATGAACATGACGCCGATGATGAATATGAAGATGACAGTCAAAATGAGAAAGACGAAATTGAGCAATTAAATGAAGATGAAACCGAGCAATGTGATGAGAAAGATGAAGACGTCGACGATGAGGATGAAACCGAGCGATGTGACGAAGAAGACGATGACGACGAACTGGAGAAAAGGATAGAAGAATTCATAGCTAAAGTGAACAAGCGATGGAGAGAAGAGAAGTTACGAGACAATTTGCTTAATCAATTTTGTTCGAGAAATGTTTATATTTGAAATTAAATTAAAATAATAAAGATTTATTATTTATGTTTTTTTGGTTTTAATAAAAAATAAAAATGATGTTTTTGTTGTTTAATTTGGTTTATTGGTTAAATAAAATTATAACAATATTTAAAATCACTTTCAAATGTCTATTTAAATTCATTTTATTTTTATTTAAATTAAAATAATAAAGGGTATATTTTTATTAATTAATTTACCTTATTTTTTACTTAGTTTTTGAGTTGCAGTCCTTTGAGCGTTCCCGTACGGATCTTCTTCTTCCTTCTTTTCTCCTCCGCTGAAGAACAACACACCGGTAAGTTCATGCCACTGATTACGCCGATGCCTTGTCTCGATCTAGGGTTTTGCGATGTTCTACTATGTATTCGTTTCTCCTAACTTAATTTTACGTTTTGATCGATTTCCTGGATAAATTTTGCTTTGACGTGCTCCGAAATCAACATCCTTTTGTTTTATTCCCTTATGGAAGTTCAAATTTGTTCTTCTGGGCCACTCCTCGATGACAATCGAATCGATTAGCCTCTGAGAATTCAATATTCTTGATTAATCACTTGGAAATCGGAATGTAGGGGCTTTCAGTTTTCGTTGCTTGCTGCTTCCTTTTGAGCGTGGACGTTTTGACATCATGGTTAATGCCATTAAAGGACTATTCATCTCCTGGTAATACTTCAAAGATAGATATCGATTTTGTTGGGCTGCACTTTCTCTTGTTTGATTATTGAGTGAAATCTAGTTTTCTTCCGTTGGTTGTTTCATCTTCTCTTTGGAATTGTTTTGACATTTGAATAACACTGCTTCTTAGAGATTAGAAACTGCCATGCCATTGTGATATTGTCCATTAATCTTTGTGATAGCTATGACATTTGAAATGACAGAGCTTAGAAAGACCAGACTGTGATGTTGTCTTCTAATACTTGGGGTCATGTTCGCTTCTTCTTCTTCTTCTTCTTTTAATCCCTATTTGCTTTGATGATCTCTTGAAACACTGGGACAGAGTCTTTGAGATCATTTTGACATTTGAATTGAGAAAGCTTCTTTAAATGACTAGAAAGTTTTACTGTCATGTTGTCTACTTTGGAGTCATGTTCTACGTTTCTTTCATCACTTTTTACTTGATGATCTCTTTTGGACATTGGGATAGGGTGTGAGATCCCACATCGGTTAGAGAGGGGAACAAAGTATTCCCTATAAGGGTATGGAAATCTCTCTCTAACCGACACGTTTTAAAACCGTGAGGGGAAGCCCAGAAGGGAAAGTCCAAAGAGAACAATATCTGCTAGCAGTGGGCTTGGGCTGTTACAAATGGTATCAAAGCCAGACACCGAGCGGTGTACCAACAAGGACGCTGGCCTTGGGTGGATTGTGGTATCCCACGTTGATTAGAGAGGGGGATGAAGCATTCCTTAGAAGGGTGTGGAAACCTCTCCCTAGTACACGCATTTTAAAACTTTGAGTGGAAGCTCAAAAGGGAAAGCTCAAAGAGGACAATATCTGCTAGCGGGGGGCATGGGCTGTTACATAGAGTCTTCAGAATTAGTATGACATTGGAAGCTACAGCGCTTTTGATTGACTAGAAACTGTCATTGTGATATTGTAGATTACTTGGGATCTTGTTCATTTCTTTTCTTTTCTCCCTGTTTTCTTGATGATCTCTTATAAACACTGGGATAGATTCATTGGAATGTTGCTATGAGTTTTTGTTTCTCATTGAATGTAAAGAGGATTTGAAGAATTATGACCTTGGCAGATTCCTTGGTTCATAAAATTTGTTATTTGCAATTGTTTGCAAAGCTTGATATATGTTTGTTATGCAACTGATATTACCTTTCTTTCCCATCTTGAATTGGGGATCCTTGATACAACTTTATCCTAGTTTTACTGAGGGAGTTTAAACTGAGATTCTAGGGATTTTTTTTTTCTTTCTGTTCTTGAGATAAATTTTATTTTCTTCTTAGATCATAGAAATGATTTTCTTCCTTGGTGCAGTGACATACCTATGGGACAATTTATCATCAACTACAATGCTTCCTTACCTGCATCCCATAGATTTATCATCCATATTTTGGATAATACTCACATGTTTGTGCAACCCAATGTAGCTGATATGATTCGAAGCGCCATGTCTGAATTTCGAGATCAGAATTCGTATGAGAAACCTACTTGAACCCCTCTGCCCCTCACTGTGTTGGTAGTGAAGGACTTCGAAGTTATAGATTTTACTTCTAAAACTTATCTTTCGTCCCAACTGTTTCACTTGTATTACTGCAATATATCTCAACAAACTTGAAGATCTGGAATGGTGGAGACTATGAGCTGCCAGTAATTCTCAACCTAAAAACGACTGATTTTGTTTTCGTAAGATATTTGTGAATATTTAATCAGTTTGTTCGAACTTCGACTATTTTTAAATAACAATTGCTTGGCATACTACATTTGGTCATGAAGAAAATATGTAATGTTATA

mRNA sequence

ATGGGAATGAGAATTATGGTGGGAGTGGTGGCTGTCATGGCGGCGGCGGCGGCGGCGGCGGCGACGGTGGTTTTGTTGGTAACTTCAAAATCATCTCAATTTTTTCTAAGTACTAAGCTTCTTGGGAGTCCTCATTTCATGTTCTCAATCTTCAACTCTATAATTCTTCTCATAATTGGGACATACCATCGGCCACCGCCTCATCAATCTCCCTATCATATACGAAACGGGTCGTGCTACCCAGGGTCGTATCAAGGGTACTATACTCCTTATCAACACATTAAATTATGTGTTCGTAACGATGAACATGACGCCGATGATGAATATGAAGATGACAGTCAAAATGAGAAAGACGAAATTGAGCAATTAAATGAAGATGAAACCGAGCAATGTGATGAGAAAGATGAAGACGTCGACGATGAGGATGAAACCGAGCGATGTGACGAAGAAGACGATGACGACGAACTGGAGAAAAGGATAGAAGAATTCATAGCTAAAGTGAACAAGCGATGGAGAGAAGAGAAGTTACGAGACAATTTGCTTAATCAATTTTGTTCGAGAAATTTTTTGAGTTGCAGTCCTTTGAGCGTTCCCGTACGGATCTTCTTCTTCCTTCTTTTCTCCTCCGCTGAAGAACAACACACCGGGGCTTTCAGTTTTCGTTGCTTGCTGCTTCCTTTTGAGCGTGGACGTTTTGACATCATGGTTAATGCCATTAAAGGACTATTCATCTCCTGTGACATACCTATGGGACAATTTATCATCAACTACAATGCTTCCTTACCTGCATCCCATAGATTTATCATCCATATTTTGGATAATACTCACATGTTTGTGCAACCCAATGTAGCTGATATGATTCGAAGCGCCATGTCTGAATTTCGAGATCAGAATTCGTATGAGAAACCTACTTGAACCCCTCTGCCCCTCACTGTGTTGGTAGTGAAGGACTTCGAAGTTATAGATTTTACTTCTAAAACTTATCTTTCGTCCCAACTGTTTCACTTGTATTACTGCAATATATCTCAACAAACTTGAAGATCTGGAATGGTGGAGACTATGAGCTGCCAGTAATTCTCAACCTAAAAACGACTGATTTTGTTTTCGTAAGATATTTGTGAATATTTAATCAGTTTGTTCGAACTTCGACTATTTTTAAATAACAATTGCTTGGCATACTACATTTGGTCATGAAGAAAATATGTAATGTTATA

Coding sequence (CDS)

ATGGGAATGAGAATTATGGTGGGAGTGGTGGCTGTCATGGCGGCGGCGGCGGCGGCGGCGGCGACGGTGGTTTTGTTGGTAACTTCAAAATCATCTCAATTTTTTCTAAGTACTAAGCTTCTTGGGAGTCCTCATTTCATGTTCTCAATCTTCAACTCTATAATTCTTCTCATAATTGGGACATACCATCGGCCACCGCCTCATCAATCTCCCTATCATATACGAAACGGGTCGTGCTACCCAGGGTCGTATCAAGGGTACTATACTCCTTATCAACACATTAAATTATGTGTTCGTAACGATGAACATGACGCCGATGATGAATATGAAGATGACAGTCAAAATGAGAAAGACGAAATTGAGCAATTAAATGAAGATGAAACCGAGCAATGTGATGAGAAAGATGAAGACGTCGACGATGAGGATGAAACCGAGCGATGTGACGAAGAAGACGATGACGACGAACTGGAGAAAAGGATAGAAGAATTCATAGCTAAAGTGAACAAGCGATGGAGAGAAGAGAAGTTACGAGACAATTTGCTTAATCAATTTTGTTCGAGAAATTTTTTGAGTTGCAGTCCTTTGAGCGTTCCCGTACGGATCTTCTTCTTCCTTCTTTTCTCCTCCGCTGAAGAACAACACACCGGGGCTTTCAGTTTTCGTTGCTTGCTGCTTCCTTTTGAGCGTGGACGTTTTGACATCATGGTTAATGCCATTAAAGGACTATTCATCTCCTGTGACATACCTATGGGACAATTTATCATCAACTACAATGCTTCCTTACCTGCATCCCATAGATTTATCATCCATATTTTGGATAATACTCACATGTTTGTGCAACCCAATGTAGCTGATATGATTCGAAGCGCCATGTCTGAATTTCGAGATCAGAATTCGTATGAGAAACCTACTTGA

Protein sequence

MGMRIMVGVVAVMAAAAAAAATVVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILLIIGTYHRPPPHQSPYHIRNGSCYPGSYQGYYTPYQHIKLCVRNDEHDADDEYEDDSQNEKDEIEQLNEDETEQCDEKDEDVDDEDETERCDEEDDDDELEKRIEEFIAKVNKRWREEKLRDNLLNQFCSRNFLSCSPLSVPVRIFFFLLFSSAEEQHTGAFSFRCLLLPFERGRFDIMVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEFRDQNSYEKPT

Homology

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy Swiss-Prot
Match: Q6NNM0 (General transcription and DNA repair factor IIH subunit TFB5 OS=Arabidopsis thaliana OX=3702 GN=TFB5 PE=3 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.0e-25
Identity = 52/70 (74.29%), Postives = 63/70 (90.00%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVNAIKG+F+SCDIPM QFI+N N S+P S +FIIH+LD+TH+FVQP+V  MIRSA+S+F
Sbjct: 1   MVNAIKGVFVSCDIPMTQFIVNMNNSMPPSQKFIIHVLDSTHLFVQPHVEQMIRSAISDF 60

Query: 295 RDQNSYEKPT 305
           RDQNSYEKPT
Sbjct: 61  RDQNSYEKPT 70

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy Swiss-Prot
Match: Q5ZKH0 (General transcription factor IIH subunit 5 OS=Gallus gallus OX=9031 GN=GTF2H5 PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 5.6e-07
Identity = 26/66 (39.39%), Postives = 39/66 (59.09%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVN +KG+ I CD  M QF++  + S     +FII  LD TH+FV   + + ++  + E 
Sbjct: 1   MVNVLKGVLIECDPAMKQFLLYLDESNALGKKFIIQDLDETHVFVLAELVNFLQERVGEL 60

Query: 295 RDQNSY 301
            DQNS+
Sbjct: 61  MDQNSF 66

[Close]

BLAST of Cp4.1LG08g07700 vs. NCBI nr
Match: KAG7028360.1 (RNA polymerase II transcription factor B subunit 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 412 bits (1060), Expect = 1.27e-142
Identity = 238/307 (77.52%), Postives = 250/307 (81.43%), Query Frame = 0


Query: 1   MGMRIMVGVVAVMAAAAAAAAT---VVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILL 60
           MGMRIMVG+VAVMAAAAAAAAT   VVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILL
Sbjct: 1   MGMRIMVGLVAVMAAAAAAAATTTVVVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILL 60

Query: 61  IIGTYHRPPPHQSPYHIRNGSCYPGSYQGYYTPYQHIKLCVRNDEHDADDEYEDDSQNEK 120
           II TYH PPPHQSPYHIR GSCY  SY  YYT  QHI+ C     H+ DDEYED++QNEK
Sbjct: 61  IIVTYHPPPPHQSPYHIRKGSCYVESYHEYYTTCQHIEFCT----HNGDDEYEDNNQNEK 120

Query: 121 DEIEQLNEDETEQCDEKDEDVDDEDETERCDEEDDDDELEKRIEEFIAKVNKRWREEKLR 180
           D+ +    DETEQCDE+DEDVDDE+ET RCDEED+DDELEKRIEEFIAKVNKRWREEKL+
Sbjct: 121 DDRDN---DETEQCDEEDEDVDDENETRRCDEEDNDDELEKRIEEFIAKVNKRWREEKLQ 180

Query: 181 DNLLNQFCSRNFLSCSPLSVPVRIFFFLLFSSAEEQHTGAFSFRCLLLPFERGRFDIMVN 240
           DNLLNQF                                AFSFRCLLLPFERGRFDIMVN
Sbjct: 181 DNLLNQF-------------------------------WAFSFRCLLLPFERGRFDIMVN 240

Query: 241 AIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEFRDQ 300
           AIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIR+AMSEFRDQ
Sbjct: 241 AIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRNAMSEFRDQ 269

Query: 301 NSYEKPT 304
           NSYEKPT
Sbjct: 301 NSYEKPT 269

[Close]

BLAST of Cp4.1LG08g07700 vs. NCBI nr
Match: KAG6596831.1 (General transcription and DNA repair factor IIH subunit TFB5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 382 bits (980), Expect = 1.11e-130
Identity = 224/306 (73.20%), Postives = 236/306 (77.12%), Query Frame = 0


Query: 1   MGMRIMVGVVAVMAAAAAAAAT--VVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILLI 60
           MGMRIMVG+VAVMAAAAAAAAT  VVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILLI
Sbjct: 1   MGMRIMVGLVAVMAAAAAAAATTVVVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILLI 60

Query: 61  IGTYHRPPPHQSPYHIRNGSCYPGSYQGYYTPYQHIKLCVRNDEHDADDEYEDDSQNEKD 120
           I TYH PPPHQSPYHIR GSCY  SY  YYT  QHI+ C     H+ DDEYED++QNEKD
Sbjct: 61  IVTYHPPPPHQSPYHIRKGSCYVESYHEYYTTCQHIEFCT----HNGDDEYEDNNQNEKD 120

Query: 121 EIEQLNEDETEQCDEKDEDVDDEDETERCDEEDDDDELEKRIEEFIAKVNKRWREEKLRD 180
           + +    DETEQCDE+DEDVDDE+ET RCDEED+DDELEKRIEEFIAK            
Sbjct: 121 DRDN---DETEQCDEEDEDVDDENETRRCDEEDNDDELEKRIEEFIAK------------ 180

Query: 181 NLLNQFCSRNFLSCSPLSVPVRIFFFLLFSSAEEQHTGAFSFRCLLLPFERGRFDIMVNA 240
                                             +HTGAFSFRCLLLPFERGRFDIMVNA
Sbjct: 181 ---------------------------------NKHTGAFSFRCLLLPFERGRFDIMVNA 240

Query: 241 IKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEFRDQN 300
           IKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIR+AMSEFRDQN
Sbjct: 241 IKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRNAMSEFRDQN 254

Query: 301 SYEKPT 304
           SYEKPT
Sbjct: 301 SYEKPT 254

[Close]

BLAST of Cp4.1LG08g07700 vs. NCBI nr
Match: XP_023005569.1 (acidic leucine-rich nuclear phosphoprotein 32 family member B-like [Cucurbita maxima])

HSP 1 Score: 281 bits (718), Expect = 7.55e-92
Identity = 162/188 (86.17%), Postives = 172/188 (91.49%), Query Frame = 0


Query: 1   MGMRIMVGVVAVMAAAAAAAATVVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILLIIG 60
           MGMRIMVG+VAVMAAAAAAA  VVLL TSKSSQ FLS+KLLGSPHFMFSIFNSIILLII 
Sbjct: 1   MGMRIMVGLVAVMAAAAAAA--VVLLATSKSSQVFLSSKLLGSPHFMFSIFNSIILLIIV 60

Query: 61  TYHRPPPHQSPYHIRNGSCYPGSYQGYYTPYQHIKLCVRNDEHDADDEYEDDSQNEKDEI 120
           TYHRPP HQSPYHIR GSCY  SYQGYYTP Q+I+    +DEHD+DDEYEDDSQNEKDEI
Sbjct: 61  TYHRPPLHQSPYHIRKGSCYLESYQGYYTPCQNIEFYACDDEHDSDDEYEDDSQNEKDEI 120

Query: 121 EQLNEDETEQCDEKDEDVDDEDETERCDEEDDDDELEKRIEEFIAKVNKRWREEKLRDNL 180
           EQLNEDETEQCDE+DEDVDDED+TERCDE+D+DDELEKRIEEFIAKVNKRWREEKLRDNL
Sbjct: 121 EQLNEDETEQCDEEDEDVDDEDDTERCDEKDNDDELEKRIEEFIAKVNKRWREEKLRDNL 180

Query: 181 LNQFCSRN 188
            NQFCS N
Sbjct: 181 FNQFCSSN 186

[Close]

BLAST of Cp4.1LG08g07700 vs. NCBI nr
Match: XP_023005607.1 (RNA polymerase II transcription factor B subunit 5 isoform X2 [Cucurbita maxima])

HSP 1 Score: 150 bits (378), Expect = 7.80e-42
Identity = 82/118 (69.49%), Postives = 83/118 (70.34%), Query Frame = 0


Query: 187 RNFLSCSPLSVPVRIFFFLLFSSAEEQHTGAFSFRCLLLPFERGRFDIMVNAIKGLFISC 246
           R FLSCSPLSV VRIFFFLLFSSAEEQ                                 
Sbjct: 4   REFLSCSPLSVLVRIFFFLLFSSAEEQTHR------------------------------ 63

Query: 247 DIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEFRDQNSYEKPT 304
           DIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIR+AMSEFRDQNSYEKPT
Sbjct: 64  DIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRNAMSEFRDQNSYEKPT 91

[Close]

BLAST of Cp4.1LG08g07700 vs. NCBI nr
Match: XP_023539253.1 (RNA polymerase II transcription factor B subunit 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147 bits (372), Expect = 3.35e-41
Identity = 70/70 (100.00%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF
Sbjct: 1   MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 60

Query: 295 RDQNSYEKPT 304
           RDQNSYEKPT
Sbjct: 61  RDQNSYEKPT 70

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy TrEMBL
Match: A0A6J1KZL9 (acidic leucine-rich nuclear phosphoprotein 32 family member B-like OS=Cucurbita maxima OX=3661 GN=LOC111498513 PE=4 SV=1)

HSP 1 Score: 281 bits (718), Expect = 3.65e-92
Identity = 162/188 (86.17%), Postives = 172/188 (91.49%), Query Frame = 0


Query: 1   MGMRIMVGVVAVMAAAAAAAATVVLLVTSKSSQFFLSTKLLGSPHFMFSIFNSIILLIIG 60
           MGMRIMVG+VAVMAAAAAAA  VVLL TSKSSQ FLS+KLLGSPHFMFSIFNSIILLII 
Sbjct: 1   MGMRIMVGLVAVMAAAAAAA--VVLLATSKSSQVFLSSKLLGSPHFMFSIFNSIILLIIV 60

Query: 61  TYHRPPPHQSPYHIRNGSCYPGSYQGYYTPYQHIKLCVRNDEHDADDEYEDDSQNEKDEI 120
           TYHRPP HQSPYHIR GSCY  SYQGYYTP Q+I+    +DEHD+DDEYEDDSQNEKDEI
Sbjct: 61  TYHRPPLHQSPYHIRKGSCYLESYQGYYTPCQNIEFYACDDEHDSDDEYEDDSQNEKDEI 120

Query: 121 EQLNEDETEQCDEKDEDVDDEDETERCDEEDDDDELEKRIEEFIAKVNKRWREEKLRDNL 180
           EQLNEDETEQCDE+DEDVDDED+TERCDE+D+DDELEKRIEEFIAKVNKRWREEKLRDNL
Sbjct: 121 EQLNEDETEQCDEEDEDVDDEDDTERCDEKDNDDELEKRIEEFIAKVNKRWREEKLRDNL 180

Query: 181 LNQFCSRN 188
            NQFCS N
Sbjct: 181 FNQFCSSN 186

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy TrEMBL
Match: A0A6J1KTL4 (RNA polymerase II transcription factor B subunit 5 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111498544 PE=3 SV=1)

HSP 1 Score: 150 bits (378), Expect = 3.78e-42
Identity = 82/118 (69.49%), Postives = 83/118 (70.34%), Query Frame = 0


Query: 187 RNFLSCSPLSVPVRIFFFLLFSSAEEQHTGAFSFRCLLLPFERGRFDIMVNAIKGLFISC 246
           R FLSCSPLSV VRIFFFLLFSSAEEQ                                 
Sbjct: 4   REFLSCSPLSVLVRIFFFLLFSSAEEQTHR------------------------------ 63

Query: 247 DIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEFRDQNSYEKPT 304
           DIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIR+AMSEFRDQNSYEKPT
Sbjct: 64  DIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRNAMSEFRDQNSYEKPT 91

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy TrEMBL
Match: A0A6J1L2N1 (RNA polymerase II transcription factor B subunit 5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498544 PE=3 SV=1)

HSP 1 Score: 146 bits (369), Expect = 4.57e-41
Identity = 69/70 (98.57%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIR+AMSEF
Sbjct: 1   MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRNAMSEF 60

Query: 295 RDQNSYEKPT 304
           RDQNSYEKPT
Sbjct: 61  RDQNSYEKPT 70

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy TrEMBL
Match: A0A6J1FX17 (RNA polymerase II transcription factor B subunit 5 OS=Cucurbita moschata OX=3662 GN=LOC111448261 PE=3 SV=1)

HSP 1 Score: 146 bits (369), Expect = 4.57e-41
Identity = 69/70 (98.57%), Postives = 70/70 (100.00%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIR+AMSEF
Sbjct: 1   MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRNAMSEF 60

Query: 295 RDQNSYEKPT 304
           RDQNSYEKPT
Sbjct: 61  RDQNSYEKPT 70

[Close]

BLAST of Cp4.1LG08g07700 vs. ExPASy TrEMBL
Match: A0A1S3BK87 (RNA polymerase II transcription factor B subunit 5 OS=Cucumis melo OX=3656 GN=LOC103490941 PE=3 SV=1)

HSP 1 Score: 136 bits (342), Expect = 5.05e-37
Identity = 63/70 (90.00%), Postives = 68/70 (97.14%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVNAIKG+FISCD+PM QFIINYNASLPASHRFIIHILDNTHMFVQP+VADMIR+A+SEF
Sbjct: 1   MVNAIKGMFISCDVPMAQFIINYNASLPASHRFIIHILDNTHMFVQPHVADMIRNAISEF 60

Query: 295 RDQNSYEKPT 304
           RDQ SYEKPT
Sbjct: 61  RDQISYEKPT 70

[Close]

BLAST of Cp4.1LG08g07700 vs. TAIR 10
Match: AT1G12400.1 (Nucleotide excision repair, TFIIH, subunit TTDA )

HSP 1 Score: 116.3 bits (290), Expect = 4.3e-26
Identity = 52/70 (74.29%), Postives = 63/70 (90.00%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHILDNTHMFVQPNVADMIRSAMSEF 294
           MVNAIKG+F+SCDIPM QFI+N N S+P S +FIIH+LD+TH+FVQP+V  MIRSA+S+F
Sbjct: 1   MVNAIKGVFVSCDIPMTQFIVNMNNSMPPSQKFIIHVLDSTHLFVQPHVEQMIRSAISDF 60

Query: 295 RDQNSYEKPT 305
           RDQNSYEKPT
Sbjct: 61  RDQNSYEKPT 70

[Close]

BLAST of Cp4.1LG08g07700 vs. TAIR 10
Match: AT1G62886.1 (Nucleotide excision repair, TFIIH, subunit TTDA )

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-17
Identity = 43/70 (61.43%), Postives = 54/70 (77.14%), Query Frame = 0


Query: 235 MVNAIKGLFISCDIPMGQFIINYNASLPASHRFIIHI--LDNTHMFVQPNVADMIRSAMS 294
           MVN+IKG+FISCD+PM QFI + N SLPAS +FII +  LD T MFV+P   +MIR A+ 
Sbjct: 1   MVNSIKGVFISCDVPMAQFIAHLNNSLPASQKFIIQVLKLDTTSMFVKPYAEEMIRDAVI 60

Query: 295 EFRDQNSYEK 303
           +FRD+NSY K
Sbjct: 61  KFRDENSYAK 70

The following BLAST results are available for this feature:

BLAST of Cp4.1LG08g07700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NNM0	6.0e-25	74.29	General transcription and DNA repair factor IIH subunit TFB5 OS=Arabidopsis thal...	[more]
Q5ZKH0	5.6e-07	39.39	General transcription factor IIH subunit 5 OS=Gallus gallus OX=9031 GN=GTF2H5 PE...	[more]

BLAST of Cp4.1LG08g07700 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028360.1	1.27e-142	77.52	RNA polymerase II transcription factor B subunit 5, partial [Cucurbita argyrospe...	[more]
KAG6596831.1	1.11e-130	73.20	General transcription and DNA repair factor IIH subunit TFB5, partial [Cucurbita...	[more]
XP_023005569.1	7.55e-92	86.17	acidic leucine-rich nuclear phosphoprotein 32 family member B-like [Cucurbita ma...	[more]
XP_023005607.1	7.80e-42	69.49	RNA polymerase II transcription factor B subunit 5 isoform X2 [Cucurbita maxima]	[more]
XP_023539253.1	3.35e-41	100.00	RNA polymerase II transcription factor B subunit 5 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Cp4.1LG08g07700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KZL9	3.65e-92	86.17	acidic leucine-rich nuclear phosphoprotein 32 family member B-like OS=Cucurbita ...	[more]
A0A6J1KTL4	3.78e-42	69.49	RNA polymerase II transcription factor B subunit 5 isoform X2 OS=Cucurbita maxim...	[more]
A0A6J1L2N1	4.57e-41	98.57	RNA polymerase II transcription factor B subunit 5 isoform X1 OS=Cucurbita maxim...	[more]
A0A6J1FX17	4.57e-41	98.57	RNA polymerase II transcription factor B subunit 5 OS=Cucurbita moschata OX=3662...	[more]
A0A1S3BK87	5.05e-37	90.00	RNA polymerase II transcription factor B subunit 5 OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of Cp4.1LG08g07700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12400.1	4.3e-26	74.29	Nucleotide excision repair, TFIIH, subunit TTDA	[more]
AT1G62886.1	1.2e-17	61.43	Nucleotide excision repair, TFIIH, subunit TTDA	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 106..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 104..154
None	No IPR available	PANTHER	PTHR28580:SF1	GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 5	coord: 235..304
IPR009400	TFIIH subunit TTDA/Tfb5	SMART	SM01395	Tbf5_2	coord: 235..302 e-value: 3.7E-32 score: 122.8
IPR009400	TFIIH subunit TTDA/Tfb5	PFAM	PF06331	Tfb5	coord: 235..302 e-value: 8.2E-25 score: 86.6
IPR009400	TFIIH subunit TTDA/Tfb5	PANTHER	PTHR28580	GENERAL TRANSCRIPTION FACTOR IIH SUBUNIT 5	coord: 235..304
IPR035935	TFB5-like superfamily	GENE3D	3.30.70.1220		coord: 235..302 e-value: 4.1E-24 score: 86.3
IPR035935	TFB5-like superfamily	SUPERFAMILY	142897	TFB5-like	coord: 240..303

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG08g07700.1	Cp4.1LG08g07700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006294	nucleotide-excision repair, preincision complex assembly
biological_process	GO:0070816	phosphorylation of RNA polymerase II C-terminal domain
biological_process	GO:0006367	transcription initiation from RNA polymerase II promoter
biological_process	GO:0006289	nucleotide-excision repair
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005669	transcription factor TFIID complex
cellular_component	GO:0000439	transcription factor TFIIH core complex
cellular_component	GO:0005675	transcription factor TFIIH holo complex