Cp4.1LG08g07420 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g07420
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionAsparagine--tRNA ligase
LocationCp4.1LG08: 5850166 .. 5859023 (-)
RNA-Seq ExpressionCp4.1LG08g07420
SyntenyCp4.1LG08g07420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TATACACTACCGCAGCAGCAGCCGCATCAGCGAAGGTCAGTCGGGTCCGCTCGTGGGAGTGGATATTTACACAACCAGTAGGAGTCACACACGGGCACAAACAAGCCTTCAATGGCTGCCGCTATGTCGCCGGCCGTTTCTCTCCGCTTCAATCCCTATTCAGCTCTCCGTCTCATCACCAGATTCCCTAAAAAACTACCACTGCACATTCTTTTCCCGCCAATTCCCCGCCGCCACTCTTCTGATTATTTCCGGCGAAGTTTTCGCACTGCCGTCTCTGCAGCTCTCTCATCCGGGGAAGCTACGGGGAAGACGAAACCTGAAGTTTCTGATGTTTGGAAGGCCTCGCTAGGGGAGAAGGTTGGGGAATTCAGAAAGAAGCTGAGGATATCTGAAGTCAAGGGCGGACTCGATGAAGGGCTGGATAGAGTCGGACAGACGCTCATCGTTAAGGGATGGGTTCGAACTCTTCGGGTTCAGAGCAGCGTTACGTTCATGGAGGTAAGGTCAGGCTATTCGTGATGTGATAGGGATTAGTTTAGAGGCTGAGAAATGCAGGATAGGAAGTTTGAGATTTTCTTTTCTATTTCCTTATCCGAATCATTTTCATTCATGTGGTGGTAAATTCTGAATGAAAACTGGAAGATAAGATTGCATTAACCATATGAGAATTGGCTTGATAATTCTTGGCCAGTCTAGTATTCTTAAGTTGTAGCGAAAAATCGCACGGCATCATTGAATGAAAACACCGTCGAATCGCATCGTGGAGCTTCTGTTATTAGCACAGTCGTAAATTTTTTTAAAGCTCTGTTTTGGTTCCATGATGATTTTATTGGTTTTAGCAATAACAAATTTAAGAGATTGGGGATATGAAACAGAGCCATCATTGACATAAATTTCAAATGGGGCAAAAGAAAAAAATGGAAACATAAAAATTGCAGTTTTAGATAGGGGATTGGATATGGATTGTTGGAAGAATAAATATTGTTTTGTATCGTGATTATCTTATTGCCTCGTTTTTTCTTGCGCTTAGGTAAATGATGGCTCATGCATTTCAAATATGCAATGTGTCATGGATTCAACTACAGAAGGTTATGATCAGGTATTCAAGTCAGCTAAATCTGGTACTTAAATAGTTGTTGAACTCTGCTTATTCTTTTTTTTTTCTTTAATAAACAATGTCAATAGCAAGCATATCCATCTTTTGAACCTAAGAAACTAACTATCTTGTAGATATTGAACTTCGAATCAAAAAATAATACACGAGAAGGCTAGAGTATATAGGAAACTGTTTGGGGTTTGAAATATGCATATTTGTTCTATAACTAACTTGCCTGATTGATAGAGTGATCAATCTTCTCTCTTTCATTTTCATTTTGGTGGTTGATATTTTGGGAGGATGATCGTGAAAGCTGTTAACTAACAGTTGGCAATAGAGAATGCAGTTATTTACGCACTTACCATTTATTTAGATGATCGTTTTATTTTCCTTGTGACAACTTTTTTTAAAAAAGAAAAGGCAATTTTGAAGATCTACAGGTATCTGAACGAACTGTTTTCTTCTTTTGCTCCTTGGAATTTTGTAGTTGAGAGAGTTAATAAGGATAACAAAAAGAGGCTCCATCAACTGTAGTAGACAATTACAAAAGAAGTTATATCATGGACACCGAGAGAGGCATTAGGATTTCTTTTAATAGGTATTTTCTTGTCTCTGTGTTCTTATTCCAGTTTTGGAGTTTGATGGCAGGTTGAATCAGGCTTGATCGCAACTGGTGCATCAGTCTGGGTGCAAGGAGTCGTGGTTGCCAGCCAAGGATCAAAACAAAAAGTGGAACTGAAACTCCAAAAAGTTGTTATGGTTAGTACTTCTCATCCTTTTTTCTCAAGTAGTTTTGGCTACAATCATTTTTCCTGATTACGAACTGCAGTATTTGCTCATATAATACATACATCCAGAAAAAAGGTGATATATATTTGAACGGCACAAGAAAAAGAATTTATTAGTACAAGGTATTAGTACGTTGGATTAACTTCATTGAAAAGGCGGTGACCATATTCATATGCGTTCGAAAGGAACCTAGAAAGAATGAAACAATTGCTAAGGGTCCATTGGACAACCATTTCTATTTTCGGCTTAAAATTTTTACATTAGAAAGTTATTGAATAATGCCTTTTTTATTGTGAACTTTAAAATTGAAAAAATCAATGTTGCTTTGTTTTGACTTTTCTAATCGATTATTCAAATGGACAGGCAAGAGTGTAAAAGGAAATTATATTTGAAGAAAGAAAAGCAGGGGAAAGTAGTGAAGAAAGTGTGTATAGGAATGGAGATGCGTACAAAATTAATAGGAAAAATGTGAGTTGAGAGAAGATAATAAGATAAAATACATGGGTAAAGAGAGATTGGTAAGAGAAGGGCTACGTTATTGGAATAAGATTCTGTGTCCCTTAAAAAAAAAATCTAAGATATCAAAAACATTTTTGAGGAATGGAAAAGAATAGCTATCAAACGTATTTGATTTTAAGAAAAGTGAAAGTTAGAAATGGAACGGTCTTCTAATAAGCTTGGATACTTGCATCAATAATTTGCTCTTTGCCTTTGTCAATACATGAAAATGGCAAACACAGTTTCTTTTAATGAAGTAAAAGTTGAGCTATTGAATTTACTTTCTTACTCTATTAATGGCTGATAAATTATATTTGATATCATGTACTTAGACTGCATCATAGATGTTCAAATTCAGAAAACATTTCCCCTTGTGTTAATATTTTCTGTATTTCAGGTTGGCAAGAGTGATCCTTCCTATCCCGTCCAAAAGAAAAGAGTTAGCAGAGAATTTTTGAGAACCAAAGCTCATCTTCGTCCAAGAACAAACACCTTTGGGGCGGTATTGTTCTGTTTTTTATTTTTATTTTTTTATTTAGTATAGATTCTAGAATACAAGGCTAGGGTGCTACTCCTGATATTCATGAAAAATAAAGGTTTCAATTGTCTAATTCACCAAAATGAACTATTTTGACATCATGGAAAATTTAGGAATGAATGGAAATCTTAAACTTTTGTGCTTTCTGATGTAGTGAATCAATGGATCAAATGTCTCAATCTTTGTTTTTTTTTGGGGGGGTTAAAATGTTGGAAGTATCATTCCTTAAAGAACATCTTTTCCTGGTGAAGATATTGATGAAACCAAAGGAGACACAAGGACATCCTCTTTTCATACGTCCTAATGATTTGAATTGTTGGATATCATTTTTTCATGGCGCTTGTATTATTCTTGGATCTTTCATAGGTTGGGAAAAGAAAACACGATGTGTTCACTTTGTTGACATAGATTTAGAGGGAAAAGTAAAACGACAGATGAATTTTGTGAAAGCATTTGTACGGAATTTGTGTTTGGAAATGAATTCTTGCCTCTTTAATAAAAGTAAGGAAAATGGATGAGGTCTTTGGCTTGGAAAAATTTCAATGCATTTTTTGTCTAATAAACTCAAATATAAGTGCCTTTATGTTAACTTTTAAAGGGAGGTTGCCTCGTCAATATATGTATGTATATGTGTGTGTATATATATATATATATATATATATATATATATATATACATATATATATATATATATAGATATGTATGTATGTATGTATATGTGTAGAAAAAGTTTGTTTCCCTCCTAAGTTTTCCGTTAGAAGATCTTTTAATTTTGCTTTGCCTCCTCCGGGTGGAGATTATTTATGGATTTCCTCTATGCTACTTAATTGTCACGTTCTTGTTTCCTGAAAATTGGTAACTGATCAAAACAATTAGTCAGACAAAATTTATGTACTGGCTTTTGTTTAACAATTTTTATGAACGGTTGTCTAATCAATCAGTTTGAATATTATGGTGTGGTATAGGTTGCAAGGGTGAGGAATGTTTTGGCATACGCTACGCACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTTTCAAGTCCTATTATTACAGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGCGTGACTACTTTGGTAGGGTCTAAACTTAGAAAAGCCTGGAATTTGTATGTTCTATTCAATATTCTTGTGGAACATTAGATTTTTTTTTTTTTTAAAAAAAACTTTTTCTGGTGGTGACAGATCCCAAGCTCTGGGGAAGCTGCTAATTCTTATGTGGGCTCCATTCCAAAGACAAAAGAGGGATTTATTGATTGGTCACAGGTGATTTTTTTTGCTAACTTAGGAAACAAGCCTTGAATCTAGTTATGTTATTTGTCAACCATCTTTTTGATTCTTACTGATGAAATTTATGGAAGATGTTAATGAGTTATTTCCAGACACTCTCTTTGAAACTTCTCCGTATTATTTTCTTTGCAAGCATTTAATTTATTCCTTTTCCATTTTTGTTTAAGGATTTTTTCGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAACGCTGAAACTTATGCTACTGCTCTCTCTGATGTATAATTTTTCTCCATTACTGGTTCTTGTTTAGACATGATGGTGGAATTAATGCAAACTGCTAATACTTTGTTCTCAAATCTTTCACAGGTTTATACTTTTGGACCGACATTTAGAGCAGAAAATTCCAATACATCCAGACATTTAGCTGAATTTTGGGTAACTAGTTTCGCATTTGTTTAATTTCCGACCAAGGTCAGTTCTGCAAGTTTTCATATGTGATAAGAAGCATCTTAAAGCAACTCTTTTACTTTATTCTTTAGATGATTGAACCAGAACTTGCATTTGCTGATTTGGATGACGACATGGCTTGTGCAACTGCATATCTCCAGTATGTAGTACGTTACCTTTCATGACTATTGTATTGTCTTAGGTTGTACTTGATTGACAAATATTTGGAAAGCTTGGATTACTTAATTTCAGAGTGACGAAAGCTTTTATTTCTAGGTGAGACATATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACATGGATTGAGAAAGGAATTATTGATCGACTGACGGTAGGAACTCTTACCATTACTTCTAGACCGTTAACAGTTATTTTCTTTATATGTAGTGTCTGTTGAAACTTTATTTACATCTATCATCTATGCCAATTTACCATTGCCATAAATTTTTCGATTTTTGTGTGCTCTGCAGGACTTGGTTGAAAAAGATTTTGTGCAGTTGACGTATACAGATGCAGTTGAGCTTCTTCTTCGAGCAAATAAGAAGTTCGAATTTCCGGTAGTGCCCACTAGTTTTATTACACTGAGCTATAGAAGTTCTGGTTCCATCTTGCACTCATTTCTCTATGATTCTATATATTGCACATTGGTAAATATTGATAGAATTCGTTTATTTATGTAGAGATGAACATACTCTATGATTAGATAATTTGAGAGATATCTAGTCTCTTGACTCGGTGCACTCTCTCAACCATGATTTCTTTCAAAACACTAACTGAGGTCACTCCTTTTTCAATCATGATTAGTCACAGTGGACCTGCAATCATTCTAACTACGCAGAAGATATATCCCTACCCTTCTTATGACCATACACTTACATAACAAATGGCCTATTCCATCCAATTCCTTACTGGGAACAAACTGTTTTAAAGGATTCTTTTGTTGAATATTACTTAAATTTATTTGTTTATTTCTTACTAATCTAGTTTGGTTCATTTCTTCCCAATCTAGTTTAGTTTTGGTGAATTATAGCATTGATATGCAAGTCAAGTTGCTTTCGTGAGTGTTATCGCTTGTTCTTGCCTCTCAATTTATTTATTTTTTTAATATTATCGGGCCAAGTTGATAAATGATATAAGATGTAACTGCCCTTGTTTTTTTACCATGTTACACACTTGGGAATCTCTGCCTTATCATTGGTGCTAATAAATCAAATGGCCAAGTATAGTAATTGCTGTGCATTGTAATGTGGTCATTGCTATGTTGATCTCAAATTTTCTAAAATTTTCTTAGGTCAAGTGGGGTAGTGATTTACAAAGCGAACATGAGCGTTATATAACTGAGGAGGCATTTGGTGGATGCCCTGTAATAATCAGAGACTATCCAAAGGTATGCTTTTCTTTGTGTTTTTAATCTTCCGAATATTGATCAAAGAAATTACACTGATTTTGCCAATTGATGTAAAATTATAGGAGATAAAAGCATTCTACATGCGGCAAAATGATGATGGAAAGACTGTTGCGGCCATGGATATGTTGGTTCCTCGGGTAATGTTAAAAGTTATAATGCCAATATATCTCTTTACCTTCCAATGGGAATGTGTTGTCTTTTTATCGTGTAACTTTTTGAAGGAATTGACTTCCAGGCATCAGCTTAGAAACAGTATGAATAGTTGGGAGAAAAGTCTAGTGGGTTATGTGGTTTCCAATTTTTGACTCTTATGCAGTCATCTTCCCCGTTTTCACTTCGTTCTTTAACTCTAGCGTTTGAGATAGAGATCCCTTTTTCATACCATTGATTGTTTCTACGCTAAAATACTATCCTTGTATTGTAAAAGACCAGGAATGCGAGGATAGTGTAAAAGGCCGTGAATGCAAGGAAAAATTTTTTTTTTTTTTTTTTGATATACATATAAGAATACGCATAAGTAATTGTTGCAATTTGAGTCAATAATTGATGCAATATGCAAGCCATTTCTACAACTGATCATCATCTTCATTGTCATGTGGGGCTGCCTTCTCTCGATTCAACATACCTTAATTTTTGTTATTGTGTCGAGCAAATTTATTTGTTATTGGTTCTACATTACAGTTGGTTTGTTTGATTAATTGACGTTCAGGTACTTATTGTATTTGAGAAAGAATACTTGATTGTATTCAAAATTTGGCAGCATGTTATTATCCACTGACCATTAATAATGTCCAAAAGAAGTTAGTACTTTTCTCGGATTTTTTCTCCAATTTATTATGTGACAATGGTGTGGGTATAGCTATAACTAATCTAGTAGCTTTCCGAGAGCATTTCTATGGACATGCAATTAAATGTTAATAATTTATTTTTCTTGTGATTACTTCTAAATATGAGAGACATCTTTTATTCTACAAATCCTTATTGTTAAAGTGAAGGTACCATATGAAGTTCTAATGCTCTTTTCTTTTTCCCCAACTCTCTCGCTTCAGATAGGGGAGCTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATCTGGAAGCTCGTCTGGATGAGTTGAAACTCAATAAAGACAGCTACTGGTGGTACCTTGACTTGCGTCGTTATGGTTCAGGTTAGAGCTGAAGCCCTCATGGTTGTGATATTAAGTTTGTTTTGTGATTAAAGGAAAAAAGGGCAAAAATGAAACAAGGAAAAACGTTTTACTTTATAGAATAATGATGTGTGTACGTGTGTGTGTTTGTGTGTGGATGTGTGTATTTTCCATTTTTCAATAAGGATAAAAGATAGGGGCATTAGAAAAAAGAACCGCCCTAACAAAGGGTCCAAAGAAACAAAACAAAAGCAAATTACAAAAAGTTGCTTCAATTGTTATGAGTTAAAAATAAAAGATGGTTGCAAAACCTTCTGGACAAATGCCAAGAGAGGCATTGAATGTATCACATTTGAAACCTCCTCTTAAGAACGCTCTAATTTAATCTCTCAAGCTAAGTATTCTNAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACATTTGAAGAACCTGACCCCTTTCCTAACAAATTCCCTCCACCAAAAGATCCTTGCATTCTCTGATAGATAGGGCTGAAAGAATGGTGGGGACAAAAAGGATAGGACGTGATTGTCATTAGTAGGAGCCCGAGAGTTTGCCTACACGAGTCATTAATATTTGGAATGAGACAAATAATCAGTAATTCAATATCGAAAGAAAATTAGGTAAAATGCGGAATCTGTTTCTTAATTCAATTGAATCTTATATTAGGTATCACAGAAATTTCAAAACTCATCACTTTCGTTAAAGCTAACTATTTGAGGATGAGATAGCAAGGTGGTCTTTTGATTTCTTCACCGTATACAAGCCAAGACTGCCTATTTTGTGGTGGTGGTGAGGGGAGTAAAAAAAGACGAGATCATCTAAAGCCTGAAAATTTTTCACCACCGGTGATGAATCTATGTCGATTTCTTAGCTAGGAAATGAAACAAACATGCAGAATTATATAGTTTTTGTAGGAAGGTGAAATAATTTGCACAAACTACTTTTTTTGGCATCAAATTTACAGCTGGGTTAGTAAGAGGATGGTGTAGGCAATAATCATTCTTGAGAGCATATGGCATATGTGATATTCCTGTGATAATTTTGGTACTAATTTCAAGTATGAACTCCAGTGCCTTGAAAAGAAAGAAAAGAGAGAAATGATTACATTGTAACCAAACCTCTTTGTGTTTCAGTTCCCCATGCAGGGTTTGGTTTGGGCTTTGAAAGGCTTGTGCAGTTTGTTACTGGAATTGATAACATAAGAGATGCCATCCCATTTCCTCGTTCGCCTGGTTCTGCAGAATTTTAATGGAAAAATTGTTCAGTAACATTATCTTGCTTTTACTCGATTCTTTTATTTAACACCCTTCCCCCCTTTTTATGGAACATGTTCTTCCTGCTCTTCATCTTTGTATTTCCATTACGTTGACACTTGACATTGAGAAAACACTGCTCTATAAGTCTGAAGGTTACAAAACTTGTGTTGCATTTATTCTATACATTGTAATAAATCATTTTTCATGTAAAACTAATGGATATGTAAAACTAATGGGTTGAATCAAATGCTTTTTCAAGCTCAATATCATTCTTAAAACTTTTACGAACTTTTACGAAGTTGGTATTTATGGATATGTAAGAAC

mRNA sequence

TATACACTACCGCAGCAGCAGCCGCATCAGCGAAGGTCAGTCGGGTCCGCTCGTGGGAGTGGATATTTACACAACCAGTAGGAGTCACACACGGGCACAAACAAGCCTTCAATGGCTGCCGCTATGTCGCCGGCCGTTTCTCTCCGCTTCAATCCCTATTCAGCTCTCCGTCTCATCACCAGATTCCCTAAAAAACTACCACTGCACATTCTTTTCCCGCCAATTCCCCGCCGCCACTCTTCTGATTATTTCCGGCGAAGTTTTCGCACTGCCGTCTCTGCAGCTCTCTCATCCGGGGAAGCTACGGGGAAGACGAAACCTGAAGTTTCTGATGTTTGGAAGGCCTCGCTAGGGGAGAAGGTTGGGGAATTCAGAAAGAAGCTGAGGATATCTGAAGTCAAGGGCGGACTCGATGAAGGGCTGGATAGAGTCGGACAGACGCTCATCGTTAAGGGATGGGTTCGAACTCTTCGGGTTCAGAGCAGCGTTACGTTCATGGAGGTAAATGATGGCTCATGCATTTCAAATATGCAATGTGTCATGGATTCAACTACAGAAGGTTATGATCAGGTTGAATCAGGCTTGATCGCAACTGGTGCATCAGTCTGGGTGCAAGGAGTCGTGGTTGCCAGCCAAGGATCAAAACAAAAAGTGGAACTGAAACTCCAAAAAGTTGTTATGGTTGGCAAGAGTGATCCTTCCTATCCCGTCCAAAAGAAAAGAGTTAGCAGAGAATTTTTGAGAACCAAAGCTCATCTTCGTCCAAGAACAAACACCTTTGGGGCGGTTGCAAGGGTGAGGAATGTTTTGGCATACGCTACGCACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTTTCAAGTCCTATTATTACAGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGCGTGACTACTTTGATCCCAAGCTCTGGGGAAGCTGCTAATTCTTATGTGGGCTCCATTCCAAAGACAAAAGAGGGATTTATTGATTGGTCACAGGATTTTTTCGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAACGCTGAAACTTATGCTACTGCTCTCTCTGATGTTTATACTTTTGGACCGACATTTAGAGCAGAAAATTCCAATACATCCAGACATTTAGCTGAATTTTGGATGATTGAACCAGAACTTGCATTTGCTGATTTGGATGACGACATGGCTTGTGCAACTGCATATCTCCAGTATGTAGTGAGACATATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACATGGATTGAGAAAGGAATTATTGATCGACTGACGGACTTGGTTGAAAAAGATTTTGTGCAGTTGACGTATACAGATGCAGTTGAGCTTCTTCTTCGAGCAAATAAGAAGTTCGAATTTCCGGTCAAGTGGGGTAGTGATTTACAAAGCGAACATGAGCGTTATATAACTGAGGAGGCATTTGGTGGATGCCCTGTAATAATCAGAGACTATCCAAAGGAGATAAAAGCATTCTACATGCGGCAAAATGATGATGGAAAGACTGTTGCGGCCATGGATATGTTGGTTCCTCGGATAGGGGAGCTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATCTGGAAGCTCGTCTGGATGAGTTGAAACTCAATAAAGACAGCTACTGGTGGTACCTTGACTTGCGTCGTTATGGTTCAGTTCCCCATGCAGGGTTTGGTTTGGGCTTTGAAAGGCTTGTGCAGTTTGTTACTGGAATTGATAACATAAGAGATGCCATCCCATTTCCTCGTTCGCCTGGTTCTGCAGAATTTTAATGGAAAAATTGTTCAGTAACATTATCTTGCTTTTACTCGATTCTTTTATTTAACACCCTTCCCCCCTTTTTATGGAACATGTTCTTCCTGCTCTTCATCTTTGTATTTCCATTACGTTGACACTTGACATTGAGAAAACACTGCTCTATAAGTCTGAAGGTTACAAAACTTGTGTTGCATTTATTCTATACATTGTAATAAATCATTTTTCATGTAAAACTAATGGATATGTAAAACTAATGGGTTGAATCAAATGCTTTTTCAAGCTCAATATCATTCTTAAAACTTTTACGAACTTTTACGAAGTTGGTATTTATGGATATGTAAGAAC

Coding sequence (CDS)

ATGGCTGCCGCTATGTCGCCGGCCGTTTCTCTCCGCTTCAATCCCTATTCAGCTCTCCGTCTCATCACCAGATTCCCTAAAAAACTACCACTGCACATTCTTTTCCCGCCAATTCCCCGCCGCCACTCTTCTGATTATTTCCGGCGAAGTTTTCGCACTGCCGTCTCTGCAGCTCTCTCATCCGGGGAAGCTACGGGGAAGACGAAACCTGAAGTTTCTGATGTTTGGAAGGCCTCGCTAGGGGAGAAGGTTGGGGAATTCAGAAAGAAGCTGAGGATATCTGAAGTCAAGGGCGGACTCGATGAAGGGCTGGATAGAGTCGGACAGACGCTCATCGTTAAGGGATGGGTTCGAACTCTTCGGGTTCAGAGCAGCGTTACGTTCATGGAGGTAAATGATGGCTCATGCATTTCAAATATGCAATGTGTCATGGATTCAACTACAGAAGGTTATGATCAGGTTGAATCAGGCTTGATCGCAACTGGTGCATCAGTCTGGGTGCAAGGAGTCGTGGTTGCCAGCCAAGGATCAAAACAAAAAGTGGAACTGAAACTCCAAAAAGTTGTTATGGTTGGCAAGAGTGATCCTTCCTATCCCGTCCAAAAGAAAAGAGTTAGCAGAGAATTTTTGAGAACCAAAGCTCATCTTCGTCCAAGAACAAACACCTTTGGGGCGGTTGCAAGGGTGAGGAATGTTTTGGCATACGCTACGCACAAGTTCTTCCAAGAAAATGGATTTGTTTGGGTTTCAAGTCCTATTATTACAGCATCAGATTGTGAAGGAGCTGGTGAACAGTTCTGCGTGACTACTTTGATCCCAAGCTCTGGGGAAGCTGCTAATTCTTATGTGGGCTCCATTCCAAAGACAAAAGAGGGATTTATTGATTGGTCACAGGATTTTTTCGGGAAACCAGCATTCTTGACTGTCTCAGGGCAACTCAACGCTGAAACTTATGCTACTGCTCTCTCTGATGTTTATACTTTTGGACCGACATTTAGAGCAGAAAATTCCAATACATCCAGACATTTAGCTGAATTTTGGATGATTGAACCAGAACTTGCATTTGCTGATTTGGATGACGACATGGCTTGTGCAACTGCATATCTCCAGTATGTAGTGAGACATATTCTTGAAAATTGCAAGGAAGACTTGGATTTCTTCAATACATGGATTGAGAAAGGAATTATTGATCGACTGACGGACTTGGTTGAAAAAGATTTTGTGCAGTTGACGTATACAGATGCAGTTGAGCTTCTTCTTCGAGCAAATAAGAAGTTCGAATTTCCGGTCAAGTGGGGTAGTGATTTACAAAGCGAACATGAGCGTTATATAACTGAGGAGGCATTTGGTGGATGCCCTGTAATAATCAGAGACTATCCAAAGGAGATAAAAGCATTCTACATGCGGCAAAATGATGATGGAAAGACTGTTGCGGCCATGGATATGTTGGTTCCTCGGATAGGGGAGCTTATAGGTGGAAGTCAAAGAGAAGAACGGCTTGATTATCTGGAAGCTCGTCTGGATGAGTTGAAACTCAATAAAGACAGCTACTGGTGGTACCTTGACTTGCGTCGTTATGGTTCAGTTCCCCATGCAGGGTTTGGTTTGGGCTTTGAAAGGCTTGTGCAGTTTGTTACTGGAATTGATAACATAAGAGATGCCATCCCATTTCCTCGTTCGCCTGGTTCTGCAGAATTTTAA

Protein sequence

MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALSSGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQKVELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLLRANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF
Homology
BLAST of Cp4.1LG08g07420 vs. ExPASy Swiss-Prot
Match: O48593 (Asparagine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SYNO PE=2 SV=3)

HSP 1 Score: 887.5 bits (2292), Expect = 8.0e-257
Identity = 441/573 (76.96%), Postives = 493/573 (86.04%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRR-------HSSDYFRRSFRT 60
           MAA   PA SLR    S LR ++ F    P + LF P+ RR         ++  RR F T
Sbjct: 1   MAATFLPATSLRLTQNSTLRFLSFFTISNPSYSLFRPLRRRVLPPFDAFPANSRRRCFCT 60

Query: 61  AVSAALSSGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIV 120
           AVS +L SG+          + ++   G KVGEFRKKLRI+EVKGG DEGL RVGQ+L +
Sbjct: 61  AVSESLGSGDG------NKVESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNI 120

Query: 121 KGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVA 180
            GWVRTLR QSSVTF+E+NDGSC+SN+QCVM S  EGYDQVESG I TGASV VQG +VA
Sbjct: 121 MGWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVA 180

Query: 181 SQGSKQKVELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVL 240
           SQG+KQKVELK++K+++VG+ D SYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRN L
Sbjct: 181 SQGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTL 240

Query: 241 AYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGF 300
           AYATHKFFQE+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E  ++ + +IPKTK G 
Sbjct: 241 AYATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGL 300

Query: 301 IDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360
           IDWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL
Sbjct: 301 IDWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360

Query: 361 AFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYT 420
           AFADLDDDMACATAYLQYVV+++L+NCKED++FF+TWIEKGII RL+D+ EK+F+QL YT
Sbjct: 361 AFADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYT 420

Query: 421 DAVELLLRANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDD 480
           DA+E+LL+ANKKF+FPVKWG DLQSEHERYITEEAFGG PVIIRDYPKEIKAFYMR+NDD
Sbjct: 421 DAIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDD 480

Query: 481 GKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAG 540
           GKTVAAMDMLVPRIGELIGGSQREERL+ LEARLDELKLNK+SYWWYLDLRRYGSVPHAG
Sbjct: 481 GKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAG 540

Query: 541 FGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           FGLGFERLVQFVTGIDNIRD IPFPR+P SAEF
Sbjct: 541 FGLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 567

BLAST of Cp4.1LG08g07420 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-163
Identity = 278/478 (58.16%), Postives = 362/478 (75.73%), Query Frame = 0

Query: 90  KLRISEVKGGLDEGLDRVGQTLIVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTE 149
           + +I  ++G   E    +G  + +KGWVRT+R Q + TF+E+NDGS +SN Q +      
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVRNQKTFTFIEINDGSTLSNFQIIATPDIA 61

Query: 150 GYDQVESGLIATGASVWVQGVVVASQGSKQKVELKLQKVVMVGKSDPS-YPVQKKRVSRE 209
           GYDQ+ + L +TG SV   G +V S G +Q +E++   + ++GK DP  Y +QKKR + E
Sbjct: 62  GYDQLINQL-STGVSVSAIGTIVESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHTFE 121

Query: 210 FLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCV 269
           FLR+ AHLRPRTNT GAV RVRN LA+ATH+FFQ+ GF+++ +PIIT SDCEGAG+ F V
Sbjct: 122 FLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMFQV 181

Query: 270 TTLIPSSGEAANSYVGSIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTF 329
           TTL  ++            +T EG +D++QDFFGKP +LTVSGQLN E YA ALSDVYTF
Sbjct: 182 TTLDQNNP----------ARTPEGRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVYTF 241

Query: 330 GPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFN 389
           GPTFRAENSNTSRHLAEFWMIEPE+AFADL+D+M CA  YL+Y+++++L+NC+ED++FFN
Sbjct: 242 GPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEFFN 301

Query: 390 TWIEKGIIDRLTDLVEKDFVQLTYTDAVELLLRANKKFEFPVKWGSDLQSEHERYITEEA 449
             +   +I RL  ++   F + +YT AV +L +A+KKFE+PVKWG DLQSEHER++ EE 
Sbjct: 302 KHVATDLISRLEHVINTSFERASYTYAVRILEKADKKFEYPVKWGLDLQSEHERFLAEEF 361

Query: 450 FGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLD 509
           FG  PVI+ DYPK+IKAFYMR N+D KTVAAMD+LVP++GE+IGGSQREERL  LE++L 
Sbjct: 362 FGK-PVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLESKLK 421

Query: 510 ELKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           E  L  + YWWYL+LR++GSVPH+GFG GFERLVQF TG++NIRD IPFPR PG A+F
Sbjct: 422 EFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKADF 467

BLAST of Cp4.1LG08g07420 vs. ExPASy Swiss-Prot
Match: P58692 (Asparagine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) OX=103690 GN=asnS PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 5.2e-155
Identity = 273/459 (59.48%), Postives = 342/459 (74.51%), Query Frame = 0

Query: 109 QTLIVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQ 168
           ++L+V+GWVRT R      F+EVNDGS + N+Q V++     Y  +   L  TGASV V 
Sbjct: 17  ESLVVQGWVRTKRELKGFAFIEVNDGSSLGNLQVVINQDLPDYAVIVKQL-NTGASVEVN 76

Query: 169 GVVVASQGSKQKVELKLQKVVMVGKSDP-SYPVQKKRVSREFLRTKAHLRPRTNTFGAVA 228
           GV+VASQG  Q++ELK + V + G++DP +YP+QKKR S EFLRT  HLR RTN+FGAV 
Sbjct: 77  GVLVASQGKGQRIELKAEAVKVYGEADPETYPLQKKRHSFEFLRTIGHLRSRTNSFGAVF 136

Query: 229 RVRNVLAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIP 288
           RVRN  + A H+FFQE GF+WV +PIITASDCEGAGE F VT+L           +  IP
Sbjct: 137 RVRNACSAAIHQFFQERGFLWVHTPIITASDCEGAGELFSVTSL----------DLKQIP 196

Query: 289 KTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFW 348
           +T+   ID+SQDFF KP +LTVSGQL AE  A A S+VYTFGPTFRAENSNTSRHLAEFW
Sbjct: 197 RTENQGIDYSQDFFAKPTYLTVSGQLEAEVMAMAFSNVYTFGPTFRAENSNTSRHLAEFW 256

Query: 349 MIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDF 408
           M+EPE+AF DL+ DM  A A+L+++  H+LE C ED++FFN  I+  ++    +++   F
Sbjct: 257 MVEPEMAFCDLEGDMDLAEAFLKHIFNHVLEKCPEDMEFFNQRIDNTVLATAENIINNQF 316

Query: 409 VQLTYTDAVELLLRANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFY 468
            +LTYTDA++LL +A+ KFE+PV WG DLQSEHERY+ E+ F   PVI+ DYP +IKAFY
Sbjct: 317 ERLTYTDAIKLLEKADVKFEYPVSWGLDLQSEHERYLAEQLFKK-PVIVTDYPAQIKAFY 376

Query: 469 MRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYG 528
           MR +DD KTV AMD+L P+IGE+IGGSQREERLD LE R+    +  +  WWYLDLRRYG
Sbjct: 377 MRLSDDEKTVRAMDVLAPKIGEIIGGSQREERLDVLERRVLAQGMQPEDLWWYLDLRRYG 436

Query: 529 SVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           +VPHAGFGLGFERLVQF+TG+ NIRD IPFPR+P +AEF
Sbjct: 437 TVPHAGFGLGFERLVQFITGMGNIRDVIPFPRTPQNAEF 463

BLAST of Cp4.1LG08g07420 vs. ExPASy Swiss-Prot
Match: A3DBI2 (Asparagine--tRNA ligase OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=asnS PE=3 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 2.0e-154
Identity = 263/462 (56.93%), Postives = 344/462 (74.46%), Query Frame = 0

Query: 105 DRVGQTLIVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGAS 164
           D VG+ ++V GWVRT+R   +  F+E+NDGS   N+Q V +      +  E   ++ G++
Sbjct: 15  DYVGKEIVVAGWVRTVRDSKAFGFIELNDGSFFKNLQIVFEEKNLP-NFKEIAKLSVGSA 74

Query: 165 VWVQGVVVASQGSKQKVELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFG 224
           +   G +V + G+KQ  ELK  K+ + G S P YP+QKKR S E+LRT AHLRPRTNTF 
Sbjct: 75  IIAHGELVETPGAKQPFELKATKIEIEGASTPDYPLQKKRHSFEYLRTIAHLRPRTNTFS 134

Query: 225 AVARVRNVLAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVG 284
           AV RVR++LAYA HKFFQE GFV+V +PIIT SD EGAG+ F VTTL           + 
Sbjct: 135 AVFRVRSLLAYAIHKFFQERGFVYVHTPIITGSDAEGAGQMFKVTTL----------DIN 194

Query: 285 SIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLA 344
           + P+ ++G +D+S+DFF +   LTVSGQL  ETY+ A  ++YTFGPTFRAENSNT+RH A
Sbjct: 195 NPPRKEDGTVDFSKDFFDRETNLTVSGQLEGETYSMAFRNIYTFGPTFRAENSNTARHAA 254

Query: 345 EFWMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVE 404
           EFWM+EPE+AFADL+DDM  A   L+Y++ + LEN  E+++FFN +I+  ++DRL ++V 
Sbjct: 255 EFWMVEPEIAFADLEDDMELAEDMLKYIINYCLENAPEEMEFFNNFIDNTLLDRLNNIVS 314

Query: 405 KDFVQLTYTDAVELLLRANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIK 464
            DF  +TYT+A+++L +A++KFE+PVKWG+DLQ+EHERY+TE+ F   PV + DYPK+IK
Sbjct: 315 SDFAHVTYTEAIDILSKADQKFEYPVKWGNDLQTEHERYLTEKVFKK-PVFVTDYPKDIK 374

Query: 465 AFYMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLR 524
           AFYMR NDD KTVAAMD+LVP +GE+IGGSQREERLDYLE R+ EL L  + YWWYLDLR
Sbjct: 375 AFYMRLNDDNKTVAAMDLLVPGVGEIIGGSQREERLDYLENRMKELGLKMEDYWWYLDLR 434

Query: 525 RYGSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           RYGS  HAGFGLGFER + ++TG+ NIRD IPFPR+  SAEF
Sbjct: 435 RYGSTRHAGFGLGFERAIMYITGMSNIRDVIPFPRTVHSAEF 464

BLAST of Cp4.1LG08g07420 vs. ExPASy Swiss-Prot
Match: B2UXS0 (Asparagine--tRNA ligase OS=Clostridium botulinum (strain Alaska E43 / Type E3) OX=508767 GN=asnS PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 2.6e-154
Identity = 258/460 (56.09%), Postives = 346/460 (75.22%), Query Frame = 0

Query: 107 VGQTLIVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVW 166
           + + +++ GW+RTLR  ++  F+E+NDGS   N+Q V D     + ++    +   +S+ 
Sbjct: 18  LSKEVMISGWIRTLRASNAFGFIEINDGSFFKNIQVVFDDKLGNFKEISK--LPISSSIS 77

Query: 167 VQGVVVASQGSKQKVELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAV 226
           V G +VA+  +KQ  E++ +++V+ G S+  YP+QKKR + E+LR+ AHLRPR+N F A 
Sbjct: 78  VVGTLVATPDAKQPFEIQAKEIVIEGMSNSDYPLQKKRHTFEYLRSIAHLRPRSNAFSAT 137

Query: 227 ARVRNVLAYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSI 286
            RVR+V A+A HKFFQE GFV+  +PIIT SDCEGAGE F VTTL P + E         
Sbjct: 138 FRVRSVAAFAIHKFFQEQGFVYTHTPIITGSDCEGAGEMFRVTTLDPKAPEL-------- 197

Query: 287 PKTKEGFIDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEF 346
             TKEG ID+++DFFGK   LTVSGQLNAE +A A  ++YTFGPTFRAENSNT+RH AEF
Sbjct: 198 --TKEGNIDYTKDFFGKETNLTVSGQLNAECFALAFRNIYTFGPTFRAENSNTTRHAAEF 257

Query: 347 WMIEPELAFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKD 406
           WMIEPE+AFADL DDM  A A L+YV++++++ C E+L FFN++++KG+++RL  +V  D
Sbjct: 258 WMIEPEIAFADLQDDMELAEAMLKYVIKYVMDECPEELQFFNSFVDKGLLERLNHVVSSD 317

Query: 407 FVQLTYTDAVELLLRANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAF 466
           F ++TYT+AVE+L + +K+F++ V WG DLQ+EHERY+TEE F   P+ + DYPKEIKAF
Sbjct: 318 FAKVTYTEAVEILEKCDKEFDYDVSWGIDLQTEHERYLTEEHFKK-PLFVTDYPKEIKAF 377

Query: 467 YMRQNDDGKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRY 526
           YMR N+D KTVAA D+LVP IGE+IGGSQREERLD LEAR+ EL L K+ YWWYL+LR+Y
Sbjct: 378 YMRMNEDNKTVAATDLLVPGIGEIIGGSQREERLDVLEARMAELGLKKEDYWWYLELRKY 437

Query: 527 GSVPHAGFGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           G   HAGFGLGFERL+ ++TG+ NIRD IPFPR+PG++EF
Sbjct: 438 GETKHAGFGLGFERLIMYITGMTNIRDVIPFPRTPGTSEF 464

BLAST of Cp4.1LG08g07420 vs. NCBI nr
Match: XP_023540678.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1137 bits (2940), Expect = 0.0
Identity = 566/566 (100.00%), Postives = 566/566 (100.00%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Cp4.1LG08g07420 vs. NCBI nr
Match: XP_022948254.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] >KAG6596861.1 Asparagine--tRNA ligase, chloroplastic/mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028390.1 Asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 562/566 (99.29%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPAVSLRFNPYSALR ITRFPKKLP HILFPPI RRHSSDYFRRSFRTAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPHILFPPISRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANS VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Cp4.1LG08g07420 vs. NCBI nr
Match: XP_023005353.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima])

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 561/566 (99.12%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPAVSLRFNPYSALR ITRFPKKLP +ILFPPIPRRHSSDYFRRSFRTAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPYILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANS VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAV+LLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVKLLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Cp4.1LG08g07420 vs. NCBI nr
Match: XP_022154023.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 525/566 (92.76%), Postives = 541/566 (95.58%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPA+SLRFNPYS LR I  FP+KLP  I  PPI RRHSS  FRRSFR AVSAALS
Sbjct: 1   MAAAMSPAISLRFNPYSTLRFIAGFPRKLPQSIPLPPITRRHSSGSFRRSFR-AVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLR+SE+KGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEAVEKTKPEVSKDWKVTRGEKVGEFRKKLRVSEIKGGLDEGLDRVGQTLVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           R QSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLI TGASVWV GVVVASQGSKQK
Sbjct: 121 RFQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLITTGASVWVHGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVG+SDPSYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGESDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVW+SSPIITASDCEGAGEQFCVTTLIP SGEAANS VG+IPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWISSPIITASDCEGAGEQFCVTTLIPGSGEAANSDVGAIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRH+LENCKEDLDFFNTWIEKG+IDRL +LVEKDFVQLTYTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHVLENCKEDLDFFNTWIEKGVIDRLNNLVEKDFVQLTYTDAIELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDS+WWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSFWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 565

BLAST of Cp4.1LG08g07420 vs. NCBI nr
Match: XP_038904123.1 (asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 1047 bits (2707), Expect = 0.0
Identity = 518/566 (91.52%), Postives = 539/566 (95.23%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPA SLRFNP S LR IT  P+KLP HILFPP+ RRHSS YFRR+F TAVS    
Sbjct: 1   MAAAMSPATSLRFNPCSTLRFITGLPRKLPQHILFPPVSRRHSSGYFRRTFCTAVS---- 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLRISE+KGGLDEGLDR+GQT +VKGWVRTL
Sbjct: 61  SGEAAEKTKPEVSKDWKVTRGEKVGEFRKKLRISEIKGGLDEGLDRLGQTFVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DST EGYDQVESGLI TGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTIEGYDQVESGLITTGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQK+V+VGKSDPSYPVQKKRVS+EFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKLVVVGKSDPSYPVQKKRVSKEFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSS EAA+S VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSEEAASSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DM+CATAYLQYVVRHILENCKED+DFFNTWIEKGIIDRLT+LVE DFVQL+YTDA+ELLL
Sbjct: 361 DMSCATAYLQYVVRHILENCKEDMDFFNTWIEKGIIDRLTNLVENDFVQLSYTDAIELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFE+PVKWGSDLQSEHERYITEEAFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEYPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 562

BLAST of Cp4.1LG08g07420 vs. ExPASy TrEMBL
Match: A0A6J1G9C9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111451973 PE=3 SV=1)

HSP 1 Score: 1125 bits (2911), Expect = 0.0
Identity = 562/566 (99.29%), Postives = 562/566 (99.29%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPAVSLRFNPYSALR ITRFPKKLP HILFPPI RRHSSDYFRRSFRTAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPHILFPPISRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANS VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Cp4.1LG08g07420 vs. ExPASy TrEMBL
Match: A0A6J1L1Y2 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111498374 PE=3 SV=1)

HSP 1 Score: 1125 bits (2909), Expect = 0.0
Identity = 561/566 (99.12%), Postives = 563/566 (99.47%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPAVSLRFNPYSALR ITRFPKKLP +ILFPPIPRRHSSDYFRRSFRTAVSAALS
Sbjct: 1   MAAAMSPAVSLRFNPYSALRFITRFPKKLPPYILFPPIPRRHSSDYFRRSFRTAVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL
Sbjct: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANS VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAV+LLL
Sbjct: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVKLLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566

BLAST of Cp4.1LG08g07420 vs. ExPASy TrEMBL
Match: A0A6J1DJ47 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111021381 PE=3 SV=1)

HSP 1 Score: 1061 bits (2743), Expect = 0.0
Identity = 525/566 (92.76%), Postives = 541/566 (95.58%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAAAMSPA+SLRFNPYS LR I  FP+KLP  I  PPI RRHSS  FRRSFR AVSAALS
Sbjct: 1   MAAAMSPAISLRFNPYSTLRFIAGFPRKLPQSIPLPPITRRHSSGSFRRSFR-AVSAALS 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
           SGEA  KTKPEVS  WK + GEKVGEFRKKLR+SE+KGGLDEGLDRVGQTL+VKGWVRTL
Sbjct: 61  SGEAVEKTKPEVSKDWKVTRGEKVGEFRKKLRVSEIKGGLDEGLDRVGQTLVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           R QSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLI TGASVWV GVVVASQGSKQK
Sbjct: 121 RFQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLITTGASVWVHGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVG+SDPSYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVVMVGESDPSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVW+SSPIITASDCEGAGEQFCVTTLIP SGEAANS VG+IPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWISSPIITASDCEGAGEQFCVTTLIPGSGEAANSDVGAIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DMACATAYLQYVVRH+LENCKEDLDFFNTWIEKG+IDRL +LVEKDFVQLTYTDA+ELLL
Sbjct: 361 DMACATAYLQYVVRHVLENCKEDLDFFNTWIEKGVIDRLNNLVEKDFVQLTYTDAIELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVPRIGELIGGSQREERLDYLEARLD+LKLNKDS+WWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPRIGELIGGSQREERLDYLEARLDDLKLNKDSFWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 565

BLAST of Cp4.1LG08g07420 vs. ExPASy TrEMBL
Match: A0A1S3BLJ5 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490884 PE=3 SV=1)

HSP 1 Score: 1035 bits (2677), Expect = 0.0
Identity = 513/566 (90.64%), Postives = 535/566 (94.52%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAA +SPA SLRFNP S LR IT  P+KL  HILFPPI RRHSS YFRRSF +AV A   
Sbjct: 1   MAAVISPATSLRFNPCSTLRFITGLPRKLSHHILFPPISRRHSSYYFRRSFCSAVPA--- 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
            GEA  KTKP+VS  WK + GEKVGEFRKKLRISE+KGG DEGLDR+GQT +VKGWVRTL
Sbjct: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGLDRLGQTFVVKGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKVVMVGKSDPSYPVQKK+VSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF
Sbjct: 181 VELKLQKVVMVGKSDPSYPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE A+S VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPASSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DM+CATAYLQYVVRH+LENCKEDLDFFNTWIEKGIIDRLT+LVE DFVQL YTDA+ELLL
Sbjct: 361 DMSCATAYLQYVVRHVLENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLD+LKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 562

BLAST of Cp4.1LG08g07420 vs. ExPASy TrEMBL
Match: A0A0A0L5I1 (Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G020020 PE=3 SV=1)

HSP 1 Score: 1026 bits (2654), Expect = 0.0
Identity = 508/566 (89.75%), Postives = 533/566 (94.17%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRRHSSDYFRRSFRTAVSAALS 60
           MAA +SPA SLRFNP S LR I+  P+KLP HILFPPI  RHSS YFRRSF +AVSA   
Sbjct: 1   MAAVISPATSLRFNPCSTLRFISGLPRKLPHHILFPPIFPRHSSCYFRRSFCSAVSA--- 60

Query: 61  SGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTL 120
            GEA  KTKP+VS  WK + GEKVGEFRKKLRISE+KGG DEG+DR+GQT +V GWVRTL
Sbjct: 61  -GEAAEKTKPDVSKDWKVTRGEKVGEFRKKLRISEIKGGRDEGVDRLGQTFVVMGWVRTL 120

Query: 121 RVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVASQGSKQK 180
           RVQSSVTFMEVNDGSCISNMQCV+DSTTEGYDQVESGLI TGASVWV+GVVVASQGSKQK
Sbjct: 121 RVQSSVTFMEVNDGSCISNMQCVIDSTTEGYDQVESGLITTGASVWVEGVVVASQGSKQK 180

Query: 181 VELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKF 240
           VELKLQKV++VGKSDPS+PVQKK+VSREFLRTKAHLRPRTNTFGAVARVRN LAYATHKF
Sbjct: 181 VELKLQKVIVVGKSDPSFPVQKKKVSREFLRTKAHLRPRTNTFGAVARVRNALAYATHKF 240

Query: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDF 300
           FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGE A S VGSIPKTKEGFIDWSQDF
Sbjct: 241 FQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEPARSDVGSIPKTKEGFIDWSQDF 300

Query: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360
           FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD
Sbjct: 301 FGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDD 360

Query: 361 DMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLL 420
           DM+CATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLT+LVE DFVQL YTDA+ELLL
Sbjct: 361 DMSCATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTNLVENDFVQLPYTDAIELLL 420

Query: 421 RANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAM 480
           RANKKFE+PVKWGSDLQSEHERYITE AFGGCPVIIRDYP++IKAFYMRQNDDGKTVAAM
Sbjct: 421 RANKKFEYPVKWGSDLQSEHERYITEVAFGGCPVIIRDYPRDIKAFYMRQNDDGKTVAAM 480

Query: 481 DMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFER 540
           DMLVP+IGELIGGSQREERLDYLEARLD+LKLNK+SYWWYLDLRRYGSVPHAGFGLGFER
Sbjct: 481 DMLVPKIGELIGGSQREERLDYLEARLDDLKLNKESYWWYLDLRRYGSVPHAGFGLGFER 540

Query: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 566
           LVQFVTGIDNIRDAIPFPRSPGSAEF
Sbjct: 541 LVQFVTGIDNIRDAIPFPRSPGSAEF 562

BLAST of Cp4.1LG08g07420 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 887.5 bits (2292), Expect = 5.7e-258
Identity = 441/573 (76.96%), Postives = 493/573 (86.04%), Query Frame = 0

Query: 1   MAAAMSPAVSLRFNPYSALRLITRFPKKLPLHILFPPIPRR-------HSSDYFRRSFRT 60
           MAA   PA SLR    S LR ++ F    P + LF P+ RR         ++  RR F T
Sbjct: 1   MAATFLPATSLRLTQNSTLRFLSFFTISNPSYSLFRPLRRRVLPPFDAFPANSRRRCFCT 60

Query: 61  AVSAALSSGEATGKTKPEVSDVWKASLGEKVGEFRKKLRISEVKGGLDEGLDRVGQTLIV 120
           AVS +L SG+          + ++   G KVGEFRKKLRI+EVKGG DEGL RVGQ+L +
Sbjct: 61  AVSESLGSGDG------NKVESYEKRFGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNI 120

Query: 121 KGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGVVVA 180
            GWVRTLR QSSVTF+E+NDGSC+SN+QCVM S  EGYDQVESG I TGASV VQG +VA
Sbjct: 121 MGWVRTLRSQSSVTFIEINDGSCLSNLQCVMTSDAEGYDQVESGSILTGASVSVQGTIVA 180

Query: 181 SQGSKQKVELKLQKVVMVGKSDPSYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVL 240
           SQG+KQKVELK++K+++VG+ D SYP+QKKRVSREFLRTKAHLRPRTNTFGAVARVRN L
Sbjct: 181 SQGTKQKVELKVEKIIVVGECDSSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTL 240

Query: 241 AYATHKFFQENGFVWVSSPIITASDCEGAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGF 300
           AYATHKFFQE+GFVWV+SPIITASDCEGAGEQFCVTTLIPSS E  ++ + +IPKTK G 
Sbjct: 241 AYATHKFFQESGFVWVASPIITASDCEGAGEQFCVTTLIPSSHENTDTSIDAIPKTKGGL 300

Query: 301 IDWSQDFFGKPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360
           IDWSQDFFGKPAFLTVSGQLN ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL
Sbjct: 301 IDWSQDFFGKPAFLTVSGQLNGETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPEL 360

Query: 361 AFADLDDDMACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYT 420
           AFADLDDDMACATAYLQYVV+++L+NCKED++FF+TWIEKGII RL+D+ EK+F+QL YT
Sbjct: 361 AFADLDDDMACATAYLQYVVKYVLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYT 420

Query: 421 DAVELLLRANKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDD 480
           DA+E+LL+ANKKF+FPVKWG DLQSEHERYITEEAFGG PVIIRDYPKEIKAFYMR+NDD
Sbjct: 421 DAIEILLKANKKFDFPVKWGLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDD 480

Query: 481 GKTVAAMDMLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAG 540
           GKTVAAMDMLVPRIGELIGGSQREERL+ LEARLDELKLNK+SYWWYLDLRRYGSVPHAG
Sbjct: 481 GKTVAAMDMLVPRIGELIGGSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAG 540

Query: 541 FGLGFERLVQFVTGIDNIRDAIPFPRSPGSAEF 567
           FGLGFERLVQFVTGIDNIRD IPFPR+P SAEF
Sbjct: 541 FGLGFERLVQFVTGIDNIRDVIPFPRTPASAEF 567

BLAST of Cp4.1LG08g07420 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 495.0 bits (1273), Expect = 8.2e-140
Identity = 265/550 (48.18%), Postives = 349/550 (63.45%), Query Frame = 0

Query: 86  EFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTLRVQSSVT--FMEVNDGSCISNMQCV 145
           +F  ++ I  + GG   G    GQ + + GWV+T R Q   T  F+EVNDGSC +N+Q +
Sbjct: 28  QFSDRVLIRSILGG---GAKLAGQKVRIGGWVKTGRQQGKGTFAFLEVNDGSCPANLQVM 87

Query: 146 MDSTTEGYDQVESGLIATGASVWVQGVV---VASQGSKQKVELKLQKVVMVGKSDP-SYP 205
           +DS+   YD   S L+ATG  V V GV+      +G KQ +EL ++ V+ VG  DP +YP
Sbjct: 88  VDSSL--YDL--SRLVATGTCVTVDGVLKIPPEGKGLKQSIELSVETVIAVGTVDPTTYP 147

Query: 206 VQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQENGFVWVSSPIITASDC 265
           + K +++ EFLR   HLR RTN   AVAR+RN LA+ATH FFQE+ F+++ +PIIT SDC
Sbjct: 148 LPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 207

Query: 266 EGAGEQFCVTTLIPSSGEAANSYVGSIPKTK----------------------------- 325
           EGAGE F VTTLI  +       + + P T+                             
Sbjct: 208 EGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVEAARLIVKERGEAVAQLKVAKASKEE 267

Query: 326 -------------------------------EGFIDWSQDFFGKPAFLTVSGQLNAETYA 385
                                          +G ID+S DFFG+ AFLTVSGQL  ETYA
Sbjct: 268 ITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKIDYSNDFFGRQAFLTVSGQLQVETYA 327

Query: 386 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILEN 445
            ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFAD+ DDM CA AY++Y+ + +++ 
Sbjct: 328 CALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADIHDDMNCAEAYVKYMCKWLMDK 387

Query: 446 CKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLLRANKK----FEFPVKWGSD 505
           C +D++  +  +++G   RL  + +  F ++TYT+A+E L +A  +    F+  V+WG D
Sbjct: 388 CGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTEAIERLEKAVAQGKVVFDNKVEWGID 447

Query: 506 LQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQ 565
           L SEHERY+TE  F   P+I+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIGGSQ
Sbjct: 448 LASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQ 507

BLAST of Cp4.1LG08g07420 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 485.7 bits (1249), Expect = 5.0e-137
Identity = 269/549 (49.00%), Postives = 347/549 (63.21%), Query Frame = 0

Query: 86  EFRKKLRISEVKGGLDEGLDRVGQTLIVKGWVRTLRVQSSVT--FMEVNDGSCISNMQCV 145
           +F  ++ I  +    D G    GQT+ + GWV++ R Q   T  F+ VNDGSC +N+Q +
Sbjct: 28  QFSNRVLIRTILDRPDGGAGLAGQTVRIGGWVKSGRDQGKRTFSFLAVNDGSCPANLQVM 87

Query: 146 MDSTTEGYDQVESGLIATGASVWVQGVVVA---SQGSKQKVELKLQKVVMVGKSDPS-YP 205
           +D +   YD   S L+ATG  V V GV+      +G++Q++EL + KV+ VG  D S YP
Sbjct: 88  VDPSL--YD--VSNLVATGTCVTVDGVLKVPPKGKGTQQQIELNVVKVIDVGTVDASKYP 147

Query: 206 VQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQENGFVWVSSPIITASDC 265
           + K +++ E LR   HLR RTN+  AVAR+RN LA+ATH FFQE+ F+++ +PIIT SDC
Sbjct: 148 LPKTKLTLETLRDVLHLRSRTNSISAVARIRNALAFATHSFFQEHSFLYIHTPIITTSDC 207

Query: 266 EGAGEQFCVTTLI-----------------------------------------PSSGEA 325
           EGAGE F  TTLI                                          +S EA
Sbjct: 208 EGAGEMFQATTLINYTERLEQDLIDNPPPTEADVEAARLIVIERGNVVAELKAAKASKEA 267

Query: 326 ANSYVGSIPKTKEGF-------------------IDWSQDFFGKPAFLTVSGQLNAETYA 385
             + V  +   KE F                   ID+S+DFFG+ AFLTVSGQL  ETYA
Sbjct: 268 ITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNIDYSKDFFGRQAFLTVSGQLQVETYA 327

Query: 386 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVRHILEN 445
            ALS+VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL+DDM CA AY++Y+   +LE 
Sbjct: 328 CALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLEDDMNCAEAYVKYMCNWLLEK 387

Query: 446 CKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLLRA---NKKFEFPVKWGSDL 505
           C  D++      + G IDRL  +    F ++TYT A+ELL  A    K+F+  V+WG DL
Sbjct: 388 CYADMELMAKNFDSGCIDRLKLVASTPFGRITYTKAIELLEEAVAKGKEFDNNVEWGIDL 447

Query: 506 QSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMDMLVPRIGELIGGSQR 565
            SEHERY+TE  F   P+I+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIGGSQR
Sbjct: 448 ASEHERYLTEVLFQK-PLIVYNYPKGIKAFYMRLNDDEKTVAAMDVLVPKVGELIGGSQR 507

BLAST of Cp4.1LG08g07420 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 339.7 bits (870), Expect = 4.4e-93
Identity = 192/503 (38.17%), Postives = 284/503 (56.46%), Query Frame = 0

Query: 126 VTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVWVQGV--VVASQGSKQKVEL 185
           V +  ++DGS IS++Q V+DS        +  L+A G  +  +GV  +  +  +K  +EL
Sbjct: 139 VFYFLISDGSSISSLQVVVDSALSTVPATQ--LMALGTCIVAEGVLRLPLAASAKHVIEL 198

Query: 186 KLQKVVMVGKSDP-SYPVQKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQ 245
           + +K++ VG  DP  YP+ KK++    LR  +H RPRT T G+V RV + L  A+H F Q
Sbjct: 199 EAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLASHTFLQ 258

Query: 246 ENGFVWVSSPIITASDCEGAGEQFCVTTLI------------------------------ 305
            +GF +V  P+IT +   G GE F VTTL+                              
Sbjct: 259 YHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEEKKPPVQEKDGFSIDTVKAVIKE 318

Query: 306 -----------PSSGEAANSYVGSIPKT-------------------KEGFIDWSQDFFG 365
                       S+ E   + V  + KT                   K   +D+S+DFFG
Sbjct: 319 KTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEKLDFSKDFFG 378

Query: 366 KPAFLTVSGQLNAETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDM 425
           +  +LT SG+ + E+YA+AL  VYTFGP F A+  + +RHLAE W +E E+AFA+LDD M
Sbjct: 379 RDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEMAFAELDDAM 438

Query: 426 ACATAYLQYVVRHILENCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLLRA 485
            CA  Y +++ +++LEN  ED+ F +  ++K I  RL        ++ +YT+ + LL +A
Sbjct: 439 DCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYTEVISLLQKA 498

Query: 486 -NKKFEFPVKWGSDLQSEHERYITEEAFGGCPVIIRDYPKEIKAFYMRQNDDGKTVAAMD 545
              KFE   +WG  L +EH  Y+T+E + G PVI+  YPK IK FY+R NDD KTVAA D
Sbjct: 499 TTTKFETKPEWGVALTTEHLSYLTDEIYKG-PVIVHTYPKAIKQFYVRLNDDKKTVAAFD 558

Query: 546 MLVPRIGELIGGSQREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFERL 565
           ++VP++G +I GSQ EER + L+AR+ E    ++ + WYLDLRR+G+V H+G  L  E++
Sbjct: 559 LVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKHSGISLSMEQM 618

BLAST of Cp4.1LG08g07420 vs. TAIR 10
Match: AT4G26870.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 4.2e-27
Identity = 116/485 (23.92%), Postives = 211/485 (43.51%), Query Frame = 0

Query: 107 VGQTLIVKGWVRTLRVQSSVTFMEVNDGSCISNMQCVMDSTTEGYDQVESGLIATGASVW 166
           VG  + ++G +   R+  +  F+ + +      +QCV++ T  G + ++     +  SV 
Sbjct: 84  VGSEVSIRGRLHKNRLVGTKLFVILRESG--FTVQCVVEETRVGANMIKFVKQLSRESV- 143

Query: 167 VQGVVVASQ------GSKQKVELKLQKVVMVGKSDPSYPV-------------------- 226
           V+ + V S       G+ Q+VE+ ++K+  + +S P+ P+                    
Sbjct: 144 VELIGVVSHPKKPLTGTTQQVEIHVRKMYCLSRSLPNLPLVVEDAARSESDIEKSGKDGK 203

Query: 227 QKKRVSREFLRTKAHLRPRTNTFGAVARVRNVLAYATHKFFQENGFVWVSSPIITASDCE 286
           Q  RV ++       L  RT    A+ R++  +  A  ++ Q  GF+ + +P + A   E
Sbjct: 204 QAARVLQDTRLNNRVLDIRTPANQAIFRIQCQVQIAFREYLQSKGFLEIHTPKLIAGSSE 263

Query: 287 GAGEQFCVTTLIPSSGEAANSYVGSIPKTKEGFIDWSQDFFGKPAFLTVSGQLNAE-TYA 346
           G    F +                              D+ G+PA L  S QL+ +    
Sbjct: 264 GGSAVFRL------------------------------DYKGQPACLAQSPQLHKQMAIC 323

Query: 347 TALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAF-ADLDDDMACATAYLQYVVRHILE 406
             +  V+  GP FRAE+S T RHL EF  ++ E+       + M        ++   I E
Sbjct: 324 GDMRRVFEVGPVFRAEDSFTHRHLCEFVGLDVEMEIRMHYSEIMDLVGELFPFIFTKIEE 383

Query: 407 NCKEDLDFFNTWIEKGIIDRLTDLVEKDFVQLTYTDAVELLLRANKKFEFPVKWGSDLQS 466
            C ++L+          +  L        ++LT+ + +++L  A ++ + P+    DL +
Sbjct: 384 RCPKELESVRKQYPFQSLKFLPQT-----LRLTFAEGIQMLKEAGEEVD-PL---GDLNT 443

Query: 467 EHERYITE---EAFGGCPVIIRDYPKEIKAFY-MRQNDDGKTVAAMDMLVPRIGELIGGS 526
           E ER + +   E +     ++  YP  ++ FY M   +D     + D+ + R  E++ G+
Sbjct: 444 ESERKLGQLVLEKYKTEFYMLHRYPSAVRPFYTMPYENDSNYSNSFDVFI-RGEEIMSGA 503

Query: 527 QREERLDYLEARLDELKLNKDSYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIRDA 560
           QR    + LE R  E  ++  +   Y+D  RYG+ PH GFG+G ER+V  +  ++NIR  
Sbjct: 504 QRIHDPELLEKRARECGIDVKTISTYIDAFRYGAPPHGGFGVGLERVVMLLCALNNIRKT 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O485938.0e-25776.96Asparagine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=... [more]
Q6MEC91.8e-16358.16Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G... [more]
P586925.2e-15559.48Asparagine--tRNA ligase OS=Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576) ... [more]
A3DBI22.0e-15456.93Asparagine--tRNA ligase OS=Hungateiclostridium thermocellum (strain ATCC 27405 /... [more]
B2UXS02.6e-15456.09Asparagine--tRNA ligase OS=Clostridium botulinum (strain Alaska E43 / Type E3) O... [more]
Match NameE-valueIdentityDescription
XP_023540678.10.0100.00asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita pepo subsp. pepo... [more]
XP_022948254.10.099.29asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita moschata] >KAG65... [more]
XP_023005353.10.099.12asparagine--tRNA ligase, chloroplastic/mitochondrial [Cucurbita maxima][more]
XP_022154023.10.092.76asparagine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia][more]
XP_038904123.10.091.52asparagine--tRNA ligase, chloroplastic/mitochondrial isoform X1 [Benincasa hispi... [more]
Match NameE-valueIdentityDescription
A0A6J1G9C90.099.29Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111451973 PE=3 SV=1[more]
A0A6J1L1Y20.099.12Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111498374 PE=3 SV=1[more]
A0A6J1DJ470.092.76Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111021381 PE=3 SV=1[more]
A0A1S3BLJ50.090.64Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103490884 PE=3 SV=1[more]
A0A0A0L5I10.089.75Asparagine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_3G020020 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G17300.15.7e-25876.96Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT1G70980.18.2e-14048.18Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT5G56680.15.0e-13749.00Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
AT3G07420.14.4e-9338.17asparaginyl-tRNA synthetase 2 [more]
AT4G26870.14.2e-2723.92Class II aminoacyl-tRNA and biotin synthetases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002312Aspartyl/Asparaginyl-tRNA synthetase, class IIbPRINTSPR01042TRNASYNTHASPcoord: 481..497
score: 45.05
coord: 307..319
score: 41.78
coord: 521..535
score: 54.7
coord: 323..336
score: 44.32
IPR004364Aminoacyl-tRNA synthetase, class II (D/K/N)PFAMPF00152tRNA-synt_2coord: 207..560
e-value: 4.3E-79
score: 265.8
NoneNo IPR availableGENE3D2.40.50.140coord: 88..196
e-value: 1.2E-15
score: 59.3
NoneNo IPR availableGENE3D3.30.930.10Bira Bifunctional Protein; Domain 2coord: 199..564
e-value: 7.7E-106
score: 356.2
NoneNo IPR availablePANTHERPTHR22594:SF46ASPARAGINE--TRNA LIGASE, CHLOROPLASTIC/MITOCHONDRIALcoord: 38..566
NoneNo IPR availablePANTHERPTHR22594ASPARTYL/LYSYL-TRNA SYNTHETASEcoord: 38..566
NoneNo IPR availableCDDcd00776AsxRS_corecoord: 204..562
e-value: 1.21529E-152
score: 438.538
NoneNo IPR availableCDDcd04318EcAsnRS_like_Ncoord: 110..193
e-value: 8.07231E-30
score: 110.348
NoneNo IPR availableSUPERFAMILY55681Class II aaRS and biotin synthetasescoord: 207..561
IPR004522Asparagine-tRNA ligaseTIGRFAMTIGR00457TIGR00457coord: 100..566
e-value: 5.1E-187
score: 620.1
IPR004522Asparagine-tRNA ligaseHAMAPMF_00534Asn_tRNA_synthcoord: 96..566
score: 44.555527
IPR006195Aminoacyl-tRNA synthetase, class IIPROSITEPS50862AA_TRNA_LIGASE_IIcoord: 228..556
score: 15.563929
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 105..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g07420.1Cp4.1LG08g07420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006421 asparaginyl-tRNA aminoacylation
biological_process GO:0006418 tRNA aminoacylation for protein translation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004816 asparagine-tRNA ligase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0004812 aminoacyl-tRNA ligase activity
molecular_function GO:0000166 nucleotide binding