Cp4.1LG08g06780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG08g06780
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	RING-type E3 ubiquitin transferase
Location	Cp4.1LG08: 4953631 .. 4955414 (+)
RNA-Seq Expression	Cp4.1LG08g06780
Synteny	Cp4.1LG08g06780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAAAGGGGGCAACCTCAAAGAACAAATTTACATGGAAATACCAATAGGTCTTGAGATAGAGGAAAGCAAACCTAAAGTCTGTGAACTTGGGAAGATCATATTCTACATTGATTTAATTTGGAAATTGTTATTAGAAACTAGATTTCTCAATCTTCTTGCATCTTTTTATGAATGAAACACGCATGTGATGTGAAATTACCTATTTTGTGCTTTCATTAATTAAATCAAGAAATAAAAGGATAAAAGTCAGCCAAGATTATTGCGTTTTCTTAGTCAGCTAAGCACATAAGACTCAACCTCGAGAAGTTATTAAACATAAAATGCCGAGATATCTATATGTAGCAAACTAACAGTCTCATTACTTAAATAATGAGGAGTTTCATGGTTGAGACTTGCAGAGAAGAGAGGAAGGATGTCGAGTAGGAACACCCATTGGTGTTACAGATGTGAGCAGCCAGTTCAACTCCGTGGGCGGGACATGGTTTGCGCTAGCTGTAATGGAGGATTTGTTCAAGAACTCGATGAAATGATGGAAAGAGGTCCCCTTGATTTCTTTGGAGCCAATGGTCATGAATACCACAATCGGAGGCTTGGACTCCTGGAGCTTTTCTCAAACTTTATGAGGCAACGGTTGATAGACAGAAATGATATGAGGGGAAGGTTGGAATCTATTCCAGATCATGGTTCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGGTTGTCTGGACATGGTGGGTTTGAAGCCTTTTTTAATGGGGTTCCTGGAATTGGTGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTTGAAGAATTGTTTGAACAGCTTTCAGCAAACGACCGACGCGGACCACCCCCAGCATCTAGATCTTCAATCGATGCAATGCCTGTTGTTAAAATTACGCAAAGACACATCCGTTCGAATTCACACTGCCCTGTTTGCCAAGATAAATTTGAATTAGGGTCTGAAGCTAGGAAAATGCCATGTGACCATATGTATCATTCGGATTGTATAGTTCCCTGGCTTGTCCAGCACAACTCCTGCCCTGTTTGCCGCCAAGAGCTGCCTGCACAAGGGTCAGGTGGCAGCCACAGCTCATCTGGAAGCAACAATCTTAATCGAAACAGCCGAGAGAGGGAGAATAGTAGGACAAATCAAGGAAGGAGGAACCCATTTTCAAGTTTATGGCCATTCCGAGCGTCGAGTTCTGACTCAAACCATAGTGCGACCACTGGAAGCAGCTCGCCAGCCGTACATGAGAGCACCCAACACCAAACAGGGTATTCTGGATGGCCTTTCGACTAGCGTGAACGGTGGTGTTTTCATGAACTTGTTTGCACCAGTTTGTGATCTTGTTGTTTAAAATGAAGAAAGATAGTTCAACCCCCTGCTGTCCACTCCTTTGTATAATGATTAATAATGGACTTTGTGTGTGATGTAGATGACATTTTTCTCTGCCTTATCGATGCTGCTGCAGTTTGTATATGGTTCATATGTATTAGCTATATGCTGTTTCGGTTTAGAGTTGCAGTCAATCTGGAATCTGTCTGAGCATTGCCGCATCTTCTTGAAATGGAAATTGAAACCTGCAAAACCGTGCATGTTTTCCGTTGTACTGTTCTTTGCCAAAGCTGTCCGTGGTCTGCATTTTCTGAACATGTTTTTGTAGGAAAACATGCAAAACTTCGTTTTGGAGAAGTTAATGCTATCATGATAGAGAGCGAGAGAAAAAAGGTAACGGATTGAATTTCTTA

mRNA sequence

ATGCAAAAAGGGGGCAACCTCAAAGAACAAATTTACATGGAAATACCAATAGAGAAGAGAGGAAGGATGTCGAGTAGGAACACCCATTGGTGTTACAGATGTGAGCAGCCAGTTCAACTCCGTGGGCGGGACATGGTTTGCGCTAGCTGTAATGGAGGATTTGTTCAAGAACTCGATGAAATGATGGAAAGAGGTCCCCTTGATTTCTTTGGAGCCAATGGTCATGAATACCACAATCGGAGGCTTGGACTCCTGGAGCTTTTCTCAAACTTTATGAGGCAACGGTTGATAGACAGAAATGATATGAGGGGAAGGTTGGAATCTATTCCAGATCATGGTTCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGGTTGTCTGGACATGGTGGGTTTGAAGCCTTTTTTAATGGGGTTCCTGGAATTGGTGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTTGAAGAATTGTTTGAACAGCTTTCAGCAAACGACCGACGCGGACCACCCCCAGCATCTAGATCTTCAATCGATGCAATGCCTGTTGTTAAAATTACGCAAAGACACATCCGTTCGAATTCACACTGCCCTGTTTGCCAAGATAAATTTGAATTAGGGTCTGAAGCTAGGAAAATGCCATGTGACCATATGTATCATTCGGATTGTATAGTTCCCTGGCTTGTCCAGCACAACTCCTGCCCTGTTTGCCGCCAAGAGCTGCCTGCACAAGGGTCAGGTGGCAGCCACAGCTCATCTGGAAGCAACAATCTTAATCGAAACAGCCGAGAGAGGGAGAATAGTAGGACAAATCAAGGAAGGAGGAACCCATTTTCAAGTTTATGGCCATTCCGAGCGTCGAGTTCTGACTCAAACCATAGTGCGACCACTGGAAGCAGCTCGCCAGCCGTACATGAGAGCACCCAACACCAAACAGGGTATTCTGGATGGCCTTTCGACTAGCGTGAACGGTGGTGTTTTCATGAACTTGTTTGCACCAGTTTGTGATCTTGTTGTTTAAAATGAAGAAAGATAGTTCAACCCCCTGCTGTCCACTCCTTTGTATAATGATTAATAATGGACTTTGTGTGTGATGTAGATGACATTTTTCTCTGCCTTATCGATGCTGCTGCAGTTTGTATATGGTTCATATGTATTAGCTATATGCTGTTTCGGTTTAGAGTTGCAGTCAATCTGGAATCTGTCTGAGCATTGCCGCATCTTCTTGAAATGGAAATTGAAACCTGCAAAACCGTGCATGTTTTCCGTTGTACTGTTCTTTGCCAAAGCTGTCCGTGGTCTGCATTTTCTGAACATGTTTTTGTAGGAAAACATGCAAAACTTCGTTTTGGAGAAGTTAATGCTATCATGATAGAGAGCGAGAGAAAAAAGGTAACGGATTGAATTTCTTA

Coding sequence (CDS)

ATGCAAAAAGGGGGCAACCTCAAAGAACAAATTTACATGGAAATACCAATAGAGAAGAGAGGAAGGATGTCGAGTAGGAACACCCATTGGTGTTACAGATGTGAGCAGCCAGTTCAACTCCGTGGGCGGGACATGGTTTGCGCTAGCTGTAATGGAGGATTTGTTCAAGAACTCGATGAAATGATGGAAAGAGGTCCCCTTGATTTCTTTGGAGCCAATGGTCATGAATACCACAATCGGAGGCTTGGACTCCTGGAGCTTTTCTCAAACTTTATGAGGCAACGGTTGATAGACAGAAATGATATGAGGGGAAGGTTGGAATCTATTCCAGATCATGGTTCAGGATTTGGTCCTTGGTTGATCTTTGGTGGCCAAATTCCTGTTAGGTTGTCTGGACATGGTGGGTTTGAAGCCTTTTTTAATGGGGTTCCTGGAATTGGTGTGAGTAGGAGCAATGGTGGTGATTATTTTATTGGTCCTGGACTTGAAGAATTGTTTGAACAGCTTTCAGCAAACGACCGACGCGGACCACCCCCAGCATCTAGATCTTCAATCGATGCAATGCCTGTTGTTAAAATTACGCAAAGACACATCCGTTCGAATTCACACTGCCCTGTTTGCCAAGATAAATTTGAATTAGGGTCTGAAGCTAGGAAAATGCCATGTGACCATATGTATCATTCGGATTGTATAGTTCCCTGGCTTGTCCAGCACAACTCCTGCCCTGTTTGCCGCCAAGAGCTGCCTGCACAAGGGTCAGGTGGCAGCCACAGCTCATCTGGAAGCAACAATCTTAATCGAAACAGCCGAGAGAGGGAGAATAGTAGGACAAATCAAGGAAGGAGGAACCCATTTTCAAGTTTATGGCCATTCCGAGCGTCGAGTTCTGACTCAAACCATAGTGCGACCACTGGAAGCAGCTCGCCAGCCGTACATGAGAGCACCCAACACCAAACAGGGTATTCTGGATGGCCTTTCGACTAG

Protein sequence

MQKGGNLKEQIYMEIPIEKRGRMSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRLGLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNGVPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGSNNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYSGWPFD

Homology

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BLAST of Cp4.1LG08g06780 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 3.5e-87
Identity = 180/327 (55.05%), Postives = 226/327 (69.11%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAN-GHEYHNRRL 83
           SSRNTHWC+RC++ V+L G++ VC  C GGFV+ELD M +  P D F ++ G    ++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 84  GLLELFSNFMRQRLIDRN---DMRGR-LESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEA 143
            L++ FS FMR RL +R+   ++RGR + S P++  G  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 144 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 203
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 204 HIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGG 263
           H+R S+S+CPVC+D+FELGSEA++MPC+H+YHSDCIVPWLVQHNSCPVCRQELP+  SG 
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS-ASGP 242

Query: 264 SHSSSGS----NNLNRNSRERENSRTN-QGRRNPFSSLWPFRASSSDSNHSATTG----- 323
           S S + +    N  + +S    NSR N   RRNPFSS WPFR+S S S+ +   G     
Sbjct: 243 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 302

Query: 324 --SSSPAVHESTQHQ---TGYSGWPFD 328
             S     +   QHQ    GYSGWPFD
Sbjct: 303 DTSDENHNYHQQQHQQSYMGYSGWPFD 327

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BLAST of Cp4.1LG08g06780 vs. ExPASy Swiss-Prot
Match: Q94AK4 (E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 2.8e-81
Identity = 175/332 (52.71%), Postives = 218/332 (65.66%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRLG 83
           S RNTHWC+RC++ V LR RD VC+ C GGFV+E+D    R   D          +    
Sbjct: 3   SIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDV-------ERDPTFD 62

Query: 84  LLELFSNFMRQRLIDRN---DMRGRLESIPDHG-SGFGPWLIFGGQIPVRLSG--HGGFE 143
           L+E FS FMR RL +R+   ++ GRL S      S   P LIFGGQ P RL+G  +   E
Sbjct: 63  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 122

Query: 144 AFFNG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKIT 203
           AF NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KIT
Sbjct: 123 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKIT 182

Query: 204 QRHIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS 263
           Q+H++ S+SHCPVC+D+FEL SEA++MPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GS
Sbjct: 183 QKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS 242

Query: 264 GGSHSSSGSNNLNRNSRERENSRTNQGRRNPFSSLWPFRASSS-------DSNHSATTGS 323
               SSS  ++ NR++  RENSR    RRN FS+LWPFR+SSS       D+N++AT   
Sbjct: 243 ----SSSTQSSQNRSTNGRENSR----RRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEE 302

Query: 324 SSPAVHESTQHQ-----------TGYSGWPFD 328
                H+  Q Q            GYSGWPFD
Sbjct: 303 GHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.1e-35
Identity = 100/296 (33.78%), Postives = 144/296 (48.65%), Query Frame = 0


Query: 30  WCYRCEQPVQLR----GRDMVCASCNGGFVQELDEMMERGPLDFFGANGHE--YHNRRLG 89
           +CY+C + V +       D  C  CN GF++E D         F   N +   +H+  L 
Sbjct: 24  FCYQCNRTVNVTISPPSSDPTCPICNEGFLEEYDNPNPNQGSGFLNPNPNSIPFHDLFLT 83

Query: 90  LLELFSNFM-------------RQRLIDRND--MRGRLESIPDHGSGFGPWLIFGGQIPV 149
           L + F++ +                 ID N+  + G   S       F P+      +  
Sbjct: 84  LSDPFASLLPLLFPSSSSTTTSSSASIDPNNPSLSGPTRSGRGDPFAFDPFTFIQNHLND 143

Query: 150 RLSGHGGFEAFFNGVPGIGVSR--SNGGDYFIGPGLEELFEQLSAND--RRGPPPASRSS 209
             S     E      P     R  +N GDYFIGPGLE+L +QL+ ND  R G PPAS+S+
Sbjct: 144 LRSSGAQIEFVIQNNPSDQGFRLPANIGDYFIGPGLEQLIQQLAENDPNRYGTPPASKSA 203

Query: 210 IDAMPVVKITQRHIRSN-SHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPV 269
           I+A+P+V IT+ ++ S  + C VC D FE G+EA++MPC H+YH DC++PWL  HNSCPV
Sbjct: 204 IEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAKQMPCKHLYHKDCLLPWLELHNSCPV 263

Query: 270 CRQELPAQGSG------GSHSSSGSNNLNRNSRERENSRTNQGRRNPFSSLWPFRA 294
           CR ELP           G+  +SG N+ + + +  +       R    S  WPF+A
Sbjct: 264 CRHELPTDDPDYERRVRGAQGTSGGNDGDNSGQRSDGDNRTVERSFRISLPWPFQA 319

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 1.7e-33
Identity = 98/310 (31.61%), Postives = 143/310 (46.13%), Query Frame = 0


Query: 30  WCYRCEQPVQL---RGRDMVCASCNGGFVQELDEMMERGPLDF----------------- 89
           +CY+C Q V +      D  C  CN GF++E ++      L+F                 
Sbjct: 22  FCYQCNQTVTISISSSADPFCPICNQGFLEEYEDPNPNQSLNFNPNSSDSFFPMADPFST 81

Query: 90  -----FGANGHEYHNRRLGLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGG 149
                FG++        +  + LF   M+         + R          F P+     
Sbjct: 82  LLPLIFGSSAAAPSG--MDFMSLFGPSMQP--------QARSTQQNPQSDAFDPFTFLQN 141

Query: 150 QIPVRLSGHGGFEAFFNGVPGIGVSR--SNGGDYFIGPGLEELFEQLSAND--RRGPPPA 209
            +    S    FE      P    +R   N GDYF GPGLE+L +QL+ ND  R G PPA
Sbjct: 142 HLQTLRSSGTHFEFVIENHPSDPGNRMPGNFGDYFFGPGLEQLIQQLAENDPNRYGTPPA 201

Query: 210 SRSSIDAMPVVKITQRHIRSN-SHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHN 269
           S+S+IDA+P VK+T+  ++S  + C VC D+FE GS+ ++MPC H++H DC++PWL  HN
Sbjct: 202 SKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVKQMPCKHVFHQDCLLPWLELHN 261

Query: 270 SCPVCRQELPAQGSGGSHSSSGSNNLNRNSRERENSRTNQGRRNPFSSL---WPFRASSS 307
           SCPVCR ELP       + S GS          +   + +G++ P  S+   WPFR    
Sbjct: 262 SCPVCRFELPTDDPDYENRSQGSQG------SGDGQGSVEGQQTPRFSIQLPWPFRRQDG 315

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.3e-28
Identity = 84/260 (32.31%), Postives = 130/260 (50.00%), Query Frame = 0


Query: 28  THWCYRCEQPVQL---RGRDMV----CASCNGGFVQELDEMMERGPLDFFGANGHEYHNR 87
           ++WCY C + V +   +G   V    C  C+GGF++++++           A+       
Sbjct: 17  SYWCYSCTRFVSVWADQGTTTVGSVACPHCDGGFIEQINDSSSAATELTIPAS------- 76

Query: 88  RLGLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGG----- 147
                E+ S    +R + R    GR  S       F P ++  G    R  G  G     
Sbjct: 77  ----TEVRSINNNRRSVIRRRRSGRRPS-------FNPVIVLQGGAGEREEGEEGDAARD 136

Query: 148 ---FEAFFNGVPGIGVS--RSNGGDYFIGPGLEELFEQLSAND-------RRGPPPASRS 207
              FE +++   G G+     +  +  +G G E L EQLS  +       R G PPAS+S
Sbjct: 137 RRAFEFYYDDGSGSGLRPLPDSVSEILMGSGFERLLEQLSQIEASATGIGRSGNPPASKS 196

Query: 208 SIDAMPVVKITQRHIRSNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPV 264
           +I+++P V+I+  HI S ++C VC + FE  +EAR+MPC H++H DCIVPWL   NSCPV
Sbjct: 197 AIESLPRVEISDCHIGSEANCAVCTEIFETETEAREMPCKHLFHDDCIVPWLSIRNSCPV 256

[Close]

BLAST of Cp4.1LG08g06780 vs. NCBI nr
Match: XP_023539608.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 635 bits (1637), Expect = 1.48e-229
Identity = 305/305 (100.00%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS
Sbjct: 241 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. NCBI nr
Match: KAG7028452.1 (putative E3 ubiquitin-protein ligase RHC1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 629 bits (1622), Expect = 2.86e-227
Identity = 302/305 (99.02%), Postives = 304/305 (99.67%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMER PLDFFGANGHEYHNRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NNL+RNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPA+HESTQHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. NCBI nr
Match: XP_022974705.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Cucurbita maxima] >XP_022974706.1 probable E3 ubiquitin-protein ligase RHC1A isoform X2 [Cucurbita maxima] >XP_022974707.1 probable E3 ubiquitin-protein ligase RHC1A isoform X3 [Cucurbita maxima] >XP_022974708.1 probable E3 ubiquitin-protein ligase RHC1A isoform X4 [Cucurbita maxima] >XP_022975226.1 probable E3 ubiquitin-protein ligase RHC1A [Cucurbita maxima])

HSP 1 Score: 627 bits (1618), Expect = 1.17e-226
Identity = 300/305 (98.36%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGA+GHEYHNRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAHGHEYHNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLL+LFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLDLFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSR+NGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRNNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NNL+RNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPA+HESTQHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. NCBI nr
Match: XP_022940469.1 (probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata])

HSP 1 Score: 625 bits (1613), Expect = 6.75e-226
Identity = 300/305 (98.36%), Postives = 303/305 (99.34%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMER PLDFFGANGHEYHNRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELG+EARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGG HSSSGS
Sbjct: 181 HCPVCQDKFELGTEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGIHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NNL+RNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPA+HESTQHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. NCBI nr
Match: XP_038904133.1 (probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Benincasa hispida])

HSP 1 Score: 589 bits (1519), Expect = 4.88e-211
Identity = 279/311 (89.71%), Postives = 296/311 (95.18%), Query Frame = 0


Query: 17  IEKRGRMSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHE 76
           + KRGRMS+ NTHWCYRCEQPV+LRGRDM+C SCNGGFVQELDEMMER P D F A+G+E
Sbjct: 28  VGKRGRMSTGNTHWCYRCEQPVRLRGRDMICPSCNGGFVQELDEMMERSPFDLFEASGNE 87

Query: 77  YHNRRLGLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGF 136
           Y NRRLGLLELFSNFMRQR++DRNDMRGRLESIPDHG GFGPWLIFGGQIPVR+SGHGGF
Sbjct: 88  YQNRRLGLLELFSNFMRQRMVDRNDMRGRLESIPDHGPGFGPWLIFGGQIPVRMSGHGGF 147

Query: 137 EAFFNGVPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQR 196
           EAFFNGVPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQR
Sbjct: 148 EAFFNGVPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQR 207

Query: 197 HIRSNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGS 256
           HIRSNSHCPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS  S
Sbjct: 208 HIRSNSHCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSVSS 267

Query: 257 HSSSGSNNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQ 316
           HSSSGSNN +RNSR+RENSRT+QGRRNPF+S+WPFRASSS+SNH ATTGSSSPA+HES Q
Sbjct: 268 HSSSGSNNSSRNSRDRENSRTSQGRRNPFTSIWPFRASSSNSNHGATTGSSSPALHESNQ 327

Query: 317 HQTGYSGWPFD 327
           HQTGY GWPFD
Sbjct: 328 HQTGYYGWPFD 338

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy TrEMBL
Match: A0A6J1IB00 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473444 PE=4 SV=1)

HSP 1 Score: 627 bits (1618), Expect = 5.65e-227
Identity = 300/305 (98.36%), Postives = 305/305 (100.00%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGA+GHEYHNRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAHGHEYHNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLL+LFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLDLFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSR+NGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRNNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NNL+RNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPA+HESTQHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy TrEMBL
Match: A0A6J1FK80 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446055 PE=4 SV=1)

HSP 1 Score: 625 bits (1613), Expect = 3.27e-226
Identity = 300/305 (98.36%), Postives = 303/305 (99.34%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMER PLDFFGANGHEYHNRRL
Sbjct: 1   MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERDPLDFFGANGHEYHNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELG+EARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGG HSSSGS
Sbjct: 181 HCPVCQDKFELGTEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGIHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NNL+RNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPA+HESTQHQTGYS
Sbjct: 241 NNLSRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPALHESTQHQTGYS 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy TrEMBL
Match: A0A0A0LCH6 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G822250 PE=4 SV=1)

HSP 1 Score: 584 bits (1505), Expect = 9.46e-210
Identity = 276/305 (90.49%), Postives = 292/305 (95.74%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MS+ NTHWCYRCEQPV+LRGRDM C SCNGGFVQELDEMMER PLD FGA+G+EY NRRL
Sbjct: 1   MSTANTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQRL+DRND+RGRL+SIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRLVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSG SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGSSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NN +RNSR+REN RT QGRRNPF+SLWPFRASSS+SNHSATTGS+SPA+HE+ QH+TGY 
Sbjct: 241 NNSSRNSRDRENGRTTQGRRNPFTSLWPFRASSSNSNHSATTGSNSPALHETNQHETGYY 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy TrEMBL
Match: A0A5A7UFI3 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G006970 PE=4 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 2.23e-208
Identity = 274/305 (89.84%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MS+ NTHWCYRCEQPV+LRGRDM C SCNGGFVQELDEMMER PLD FGA+G+EY NRRL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQR++DRND+RGRL+SIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS  SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NN +RNSR+RENSRT QGRRNPF+SLWPFRASSS+SNH ATTGS+SPA+HE+ QH+TGY 
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. ExPASy TrEMBL
Match: A0A1S3B774 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486912 PE=4 SV=1)

HSP 1 Score: 580 bits (1496), Expect = 2.23e-208
Identity = 274/305 (89.84%), Postives = 291/305 (95.41%), Query Frame = 0


Query: 23  MSSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRL 82
           MS+ NTHWCYRCEQPV+LRGRDM C SCNGGFVQELDEMMER PLD FGA+G+EY NRRL
Sbjct: 1   MSTGNTHWCYRCEQPVRLRGRDMTCLSCNGGFVQELDEMMERSPLDLFGASGNEYQNRRL 60

Query: 83  GLLELFSNFMRQRLIDRNDMRGRLESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEAFFNG 142
           GLLELFSNFMRQR++DRND+RGRL+SIPDHG GFGPWLIFGGQIPVRLSGHGGFEAFFNG
Sbjct: 61  GLLELFSNFMRQRMVDRNDIRGRLDSIPDHGPGFGPWLIFGGQIPVRLSGHGGFEAFFNG 120

Query: 143 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 202
           VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS
Sbjct: 121 VPGIGVSRSNGGDYFIGPGLEELFEQLSANDRRGPPPASRSSIDAMPVVKITQRHIRSNS 180

Query: 203 HCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGGSHSSSGS 262
           HCPVCQDKFELGSEAR+MPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS  SHSSSGS
Sbjct: 181 HCPVCQDKFELGSEARQMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSSSSHSSSGS 240

Query: 263 NNLNRNSRERENSRTNQGRRNPFSSLWPFRASSSDSNHSATTGSSSPAVHESTQHQTGYS 322
           NN +RNSR+RENSRT QGRRNPF+SLWPFRASSS+SNH ATTGS+SPA+HE+ QH+TGY 
Sbjct: 241 NNSSRNSRDRENSRTTQGRRNPFTSLWPFRASSSNSNHGATTGSNSPALHETNQHETGYY 300

Query: 323 GWPFD 327
           GWPFD
Sbjct: 301 GWPFD 305

[Close]

BLAST of Cp4.1LG08g06780 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 323.2 bits (827), Expect = 2.5e-88
Identity = 180/327 (55.05%), Postives = 226/327 (69.11%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAN-GHEYHNRRL 83
           SSRNTHWC+RC++ V+L G++ VC  C GGFV+ELD M +  P D F ++ G    ++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 84  GLLELFSNFMRQRLIDRN---DMRGR-LESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEA 143
            L++ FS FMR RL +R+   ++RGR + S P++  G  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 144 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 203
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 204 HIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGG 263
           H+R S+S+CPVC+D+FELGSEA++MPC+H+YHSDCIVPWLVQHNSCPVCRQELP+  SG 
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS-ASGP 242

Query: 264 SHSSSGS----NNLNRNSRERENSRTN-QGRRNPFSSLWPFRASSSDSNHSATTG----- 323
           S S + +    N  + +S    NSR N   RRNPFSS WPFR+S S S+ +   G     
Sbjct: 243 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 302

Query: 324 --SSSPAVHESTQHQ---TGYSGWPFD 328
             S     +   QHQ    GYSGWPFD
Sbjct: 303 DTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Cp4.1LG08g06780 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 323.2 bits (827), Expect = 2.5e-88
Identity = 180/327 (55.05%), Postives = 226/327 (69.11%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAN-GHEYHNRRL 83
           SSRNTHWC+RC++ V+L G++ VC  C GGFV+ELD M +  P D F ++ G    ++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 84  GLLELFSNFMRQRLIDRN---DMRGR-LESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEA 143
            L++ FS FMR RL +R+   ++RGR + S P++  G  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 144 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 203
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 204 HIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGG 263
           H+R S+S+CPVC+D+FELGSEA++MPC+H+YHSDCIVPWLVQHNSCPVCRQELP+  SG 
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS-ASGP 242

Query: 264 SHSSSGS----NNLNRNSRERENSRTN-QGRRNPFSSLWPFRASSSDSNHSATTG----- 323
           S S + +    N  + +S    NSR N   RRNPFSS WPFR+S S S+ +   G     
Sbjct: 243 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 302

Query: 324 --SSSPAVHESTQHQ---TGYSGWPFD 328
             S     +   QHQ    GYSGWPFD
Sbjct: 303 DTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Cp4.1LG08g06780 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 323.2 bits (827), Expect = 2.5e-88
Identity = 180/327 (55.05%), Postives = 226/327 (69.11%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGAN-GHEYHNRRL 83
           SSRNTHWC+RC++ V+L G++ VC  C GGFV+ELD M +  P D F ++ G    ++  
Sbjct: 3   SSRNTHWCHRCQRAVRLHGQEPVCFYCGGGFVEELD-MAQASPFDMFRSHRGVVERDQTF 62

Query: 84  GLLELFSNFMRQRLIDRN---DMRGR-LESIPDHGSGFGPWLIFGGQIPVRLSGHGGFEA 143
            L++ FS FMR RL +R+   ++RGR + S P++  G  P LIFGGQ+P RL+G    EA
Sbjct: 63  DLMDAFSVFMRNRLAERSHDREIRGRTISSGPENFPGLAPLLIFGGQVPYRLTGDNAVEA 122

Query: 144 FFN-GVPGIGVSRSNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKITQR 203
            FN G PGIG++R N GDYF GPGLEELFEQLSA   RRGPPPA RS+IDA+P +KI QR
Sbjct: 123 LFNGGSPGIGITRGNTGDYFFGPGLEELFEQLSAGTTRRGPPPAPRSAIDALPTIKIAQR 182

Query: 204 HIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGSGG 263
           H+R S+S+CPVC+D+FELGSEA++MPC+H+YHSDCIVPWLVQHNSCPVCRQELP+  SG 
Sbjct: 183 HLRSSDSNCPVCKDEFELGSEAKQMPCNHIYHSDCIVPWLVQHNSCPVCRQELPS-ASGP 242

Query: 264 SHSSSGS----NNLNRNSRERENSRTN-QGRRNPFSSLWPFRASSSDSNHSATTG----- 323
           S S + +    N  + +S    NSR N   RRNPFSS WPFR+S S S+ +   G     
Sbjct: 243 SSSQNRTTPTRNYRSSSSSSSSNSRENGNERRNPFSSFWPFRSSGSSSSSTQNRGGTRNS 302

Query: 324 --SSSPAVHESTQHQ---TGYSGWPFD 328
             S     +   QHQ    GYSGWPFD
Sbjct: 303 DTSDENHNYHQQQHQQSYMGYSGWPFD 327

[Close]

BLAST of Cp4.1LG08g06780 vs. TAIR 10
Match: AT3G56580.2 (RING/U-box superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.0e-82
Identity = 175/332 (52.71%), Postives = 218/332 (65.66%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRLG 83
           S RNTHWC+RC++ V LR RD VC+ C GGFV+E+D    R   D          +    
Sbjct: 3   SIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDV-------ERDPTFD 62

Query: 84  LLELFSNFMRQRLIDRN---DMRGRLESIPDHG-SGFGPWLIFGGQIPVRLSG--HGGFE 143
           L+E FS FMR RL +R+   ++ GRL S      S   P LIFGGQ P RL+G  +   E
Sbjct: 63  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 122

Query: 144 AFFNG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKIT 203
           AF NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KIT
Sbjct: 123 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKIT 182

Query: 204 QRHIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS 263
           Q+H++ S+SHCPVC+D+FEL SEA++MPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GS
Sbjct: 183 QKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS 242

Query: 264 GGSHSSSGSNNLNRNSRERENSRTNQGRRNPFSSLWPFRASSS-------DSNHSATTGS 323
               SSS  ++ NR++  RENSR    RRN FS+LWPFR+SSS       D+N++AT   
Sbjct: 243 ----SSSTQSSQNRSTNGRENSR----RRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEE 302

Query: 324 SSPAVHESTQHQ-----------TGYSGWPFD 328
                H+  Q Q            GYSGWPFD
Sbjct: 303 GHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

[Close]

BLAST of Cp4.1LG08g06780 vs. TAIR 10
Match: AT3G56580.1 (RING/U-box superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.0e-82
Identity = 175/332 (52.71%), Postives = 218/332 (65.66%), Query Frame = 0


Query: 24  SSRNTHWCYRCEQPVQLRGRDMVCASCNGGFVQELDEMMERGPLDFFGANGHEYHNRRLG 83
           S RNTHWC+RC++ V LR RD VC+ C GGFV+E+D    R   D          +    
Sbjct: 3   SIRNTHWCHRCQRAVWLRARDAVCSYCGGGFVEEIDIGPSRAHRDV-------ERDPTFD 62

Query: 84  LLELFSNFMRQRLIDRN---DMRGRLESIPDHG-SGFGPWLIFGGQIPVRLSG--HGGFE 143
           L+E FS FMR RL +R+   ++ GRL S      S   P LIFGGQ P RL+G  +   E
Sbjct: 63  LMEAFSAFMRSRLAERSYDREISGRLGSAGSESFSNLAPLLIFGGQAPFRLAGGDNSSVE 122

Query: 144 AFFNG-VPGIGVSR-SNGGDYFIGPGLEELFEQLSA-NDRRGPPPASRSSIDAMPVVKIT 203
           AF NG  PGIG++R +N GDYF GPGLEEL EQLS+    RGPPPA +SSIDA+P +KIT
Sbjct: 123 AFVNGAAPGIGIARGTNAGDYFFGPGLEELIEQLSSGTHHRGPPPAPKSSIDALPTIKIT 182

Query: 204 QRHIR-SNSHCPVCQDKFELGSEARKMPCDHMYHSDCIVPWLVQHNSCPVCRQELPAQGS 263
           Q+H++ S+SHCPVC+D+FEL SEA++MPC H+YHSDCIVPWLVQHNSCPVCR+ELP++GS
Sbjct: 183 QKHLKSSDSHCPVCKDEFELKSEAKQMPCHHIYHSDCIVPWLVQHNSCPVCRKELPSRGS 242

Query: 264 GGSHSSSGSNNLNRNSRERENSRTNQGRRNPFSSLWPFRASSS-------DSNHSATTGS 323
               SSS  ++ NR++  RENSR    RRN FS+LWPFR+SSS       D+N++AT   
Sbjct: 243 ----SSSTQSSQNRSTNGRENSR----RRNIFSNLWPFRSSSSSSTQNRRDTNNTATAEE 302

Query: 324 SSPAVHESTQHQ-----------TGYSGWPFD 328
                H+  Q Q            GYSGWPFD
Sbjct: 303 GHYHHHQQQQQQHQHQHQQQQSHMGYSGWPFD 319

The following BLAST results are available for this feature:

BLAST of Cp4.1LG08g06780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	3.5e-87	55.05	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q94AK4	2.8e-81	52.71	E3 ubiquitin-protein ligase RZF1 OS=Arabidopsis thaliana OX=3702 GN=RZF1 PE=1 SV...	[more]
P0CH30	1.1e-35	33.78	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q8LPN7	1.7e-33	31.61	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
Q940T5	1.3e-28	32.31	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]

BLAST of Cp4.1LG08g06780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023539608.1	1.48e-229	100.00	probable E3 ubiquitin-protein ligase RHC1A [Cucurbita pepo subsp. pepo]	[more]
KAG7028452.1	2.86e-227	99.02	putative E3 ubiquitin-protein ligase RHC1A, partial [Cucurbita argyrosperma subs...	[more]
XP_022974705.1	1.17e-226	98.36	probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Cucurbita maxima] >XP_022...	[more]
XP_022940469.1	6.75e-226	98.36	probable E3 ubiquitin-protein ligase RHC1A [Cucurbita moschata]	[more]
XP_038904133.1	4.88e-211	89.71	probable E3 ubiquitin-protein ligase RHC1A isoform X1 [Benincasa hispida]	[more]

BLAST of Cp4.1LG08g06780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IB00	5.65e-227	98.36	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111473444 P...	[more]
A0A6J1FK80	3.27e-226	98.36	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446055...	[more]
A0A0A0LCH6	9.46e-210	90.49	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_3G822250 PE...	[more]
A0A5A7UFI3	2.23e-208	89.84	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3B774	2.23e-208	89.84	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103486912 PE=4 ...	[more]

BLAST of Cp4.1LG08g06780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40830.2	2.5e-88	55.05	RING-H2 finger C1A	[more]
AT2G40830.1	2.5e-88	55.05	RING-H2 finger C1A	[more]
AT2G40830.3	2.5e-88	55.05	RING-H2 finger C1A	[more]
AT3G56580.2	2.0e-82	52.71	RING/U-box superfamily protein	[more]
AT3G56580.1	2.0e-82	52.71	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 204..244 e-value: 2.4E-6 score: 37.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 203..245 e-value: 1.2E-11 score: 44.8
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 204..245 score: 12.061441
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 166..251 e-value: 3.1E-22 score: 80.0
IPR039525	E3 ubiquitin-protein ligase RNF126-like, zinc-ribbon	PFAM	PF14369	zinc_ribbon_9	coord: 27..58 e-value: 7.5E-9 score: 35.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 249..327
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 279..327
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 251..268
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 24..323
None	No IPR available	PANTHER	PTHR15710:SF83	E3 UBIQUITIN-PROTEIN LIGASE RHC1A-RELATED	coord: 24..323
None	No IPR available	CDD	cd16667	RING-H2_RNF126_like	coord: 204..245 e-value: 1.05058E-23 score: 89.6844
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 184..251

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG08g06780.1	Cp4.1LG08g06780.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity