Cp4.1LG08g06350 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG08g06350 vs. ExPASy Swiss-Prot
Match: Q55CU8 (Phosphatidylinositol transfer protein 3 OS=Dictyostelium discoideum OX=44689 GN=pitC PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 1.2e-19
Identity = 61/205 (29.76%), Postives = 102/205 (49.76%), Query Frame = 0

Query: 79  DFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASEISTNLAHNKLFMQGVDKK 138
           D    R+LRAR+  + K+  M    L WRK   P       +I    +   +++   DKK
Sbjct: 73  DMCFLRYLRARNYIVSKSEKMLRDTLEWRKKFRPQDIQLGGDIREIGSAGCVYVNKRDKK 132

Query: 139 GRPIVVGYGNRHKQGNMEEFIRY--VIFALEKICSRM--PRGQEKFVCIGDLQGWGYSNS 198
           GRPI+          N+   +++  +++ LE+  SRM  P+G E+F  I D + +G  N 
Sbjct: 133 GRPIIFAVPRNDTLKNVPSELKFKNLVYWLEQGFSRMDEPKGIEQFCFIVDYKDFGSGNM 192

Query: 199 DIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLR 258
           D++    ++  L D  PER+G+   +  P +F  AWK++ PF+++ T  K++F+  KK+ 
Sbjct: 193 DMKTNLEAMHFLLDHCPERMGQSLFLDPPALFWFAWKIISPFLNEVTLSKVRFINSKKVD 252

Query: 259 -----STLLSDIDESQLPDVYGGKL 275
                + LL  +D   L    GG L
Sbjct: 253 GKRTFAELLEYVDIENLEQNLGGNL 277

BLAST of Cp4.1LG08g06350 vs. ExPASy Swiss-Prot
Match: P33324 (CRAL-TRIO domain-containing protein YKL091C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YKL091C PE=1 SV=2)

HSP 1 Score: 89.4 bits (220), Expect = 7.3e-17
Identity = 74/242 (30.58%), Postives = 116/242 (47.93%), Query Frame = 0

Query: 56  EKQKVVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGF 115
           E+++ ++    +  E +  + +DD  + RFLRAR  DI  +  MF++   WR+    N  
Sbjct: 28  EQEEALLQFRSILLEKNYKERLDDSTLLRFLRARKFDINASVEMFVETERWREEYGANTI 87

Query: 116 I------SASEISTNLAHNKLFMQ---GVDKKGRPIV------VGYGNRHKQGNMEEFIR 175
           I        +E    +   K++ Q    VDK GRP+       +     +K    ++ +R
Sbjct: 88  IEDYENNKEAEDKERIKLAKMYPQYYHHVDKDGRPLYFEELGGINLKKMYKITTEKQMLR 147

Query: 176 -----YVIFALEKI--CSRMPRGQEKFVC-IGDLQGWGYSNS-DIRGYRASLS-ILQDCY 235
                Y +FA  ++  CSR      +  C + DL+G   SN+  +  Y   ++ I Q+ Y
Sbjct: 148 NLVKEYELFATYRVPACSRRAGYLIETSCTVLDLKGISLSNAYHVLSYIKDVADISQNYY 207

Query: 236 PERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVY 273
           PER+GK YI+H P+ F T +KMV PF+D  T  KI F+     +  LL  I    LP  Y
Sbjct: 208 PERMGKFYIIHSPFGFSTMFKMVKPFLDPVTVSKI-FILGSSYKKELLKQIPIENLPVKY 267

BLAST of Cp4.1LG08g06350 vs. ExPASy Swiss-Prot
Match: Q10137 (Sec14 cytosolic factor OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sec14 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 9.5e-17
Identity = 76/252 (30.16%), Postives = 120/252 (47.62%), Query Frame = 0

Query: 40  GSVTPVFEDSLESNEVEKQKVVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAM 99
           G +    + +L+S  +E QK+     + ER       +DD  + RFLRAR  +++++  M
Sbjct: 22  GHLNSTQQATLDSMRLELQKL----GYTER-------LDDATLLRFLRARKFNLQQSLEM 81

Query: 100 FLKYLSWRKSAIPNGFISASEISTNLAHNKLFMQ---GVDKKGRPIVVG----------Y 159
           F+K   WRK    +  I         A +K + Q     D  GRP+ V           Y
Sbjct: 82  FIKCEKWRKEFGVDDLIKNFHYDEKEAVSKYYPQFYHKTDIDGRPVYVEQLGNIDLKKLY 141

Query: 160 GNRHKQGNMEEFI-RYVIFALEKI--CSRMPRGQEKFVC-IGDLQGWGYSN-SDIRGY-R 219
                +  M+  +  Y + AL++   CSR   G  +  C I DL+G G ++   +  Y R
Sbjct: 142 QITTPERMMQNLVYEYEMLALKRFPACSRKAGGLIETSCTIMDLKGVGITSIHSVYSYIR 201

Query: 220 ASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSD 273
            + SI QD YPER+GK Y+++ P+ F +A+ ++  F+D+ T KKI  +     +S LL  
Sbjct: 202 QASSISQDYYPERMGKFYVINAPWGFSSAFNLIKGFLDEATVKKIHIL-GSNYKSALLEQ 261

BLAST of Cp4.1LG08g06350 vs. ExPASy Swiss-Prot
Match: F4JVA9 (Phosphatidylinositol/phosphatidylcholine transfer protein SFH2 OS=Arabidopsis thaliana OX=3702 GN=SFH2 PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 3.6e-16
Identity = 78/260 (30.00%), Postives = 118/260 (45.38%), Query Frame = 0

Query: 48  DSLESNEVEKQKVVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWR 107
           D +++ E++       +A +  E   +K  D  M+ RFLRAR  D+EKA  M+   L+WR
Sbjct: 64  DEIDTEELQAVD-AFRQALILDELLPSKHDDHHMMLRFLRARKFDLEKAKQMWSDMLNWR 123

Query: 108 K----SAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIV------VGYGNRHKQGNMEE 167
           K      I   F    EI   + +      GVDK+GRPI       V      K   ++ 
Sbjct: 124 KEYGADTIMEDF-DFKEIEEVVKYYPQGYHGVDKEGRPIYIERLGQVDATKLMKVTTIDR 183

Query: 168 FIRYVIFALEK-------ICS-RMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQ-- 227
           +++Y +   EK        CS    R  ++   I D+QG G SN +    +A+  +LQ  
Sbjct: 184 YVKYHVKEFEKTFNVKFPACSIAAKRHIDQSTTILDVQGVGLSNFN----KAAKDLLQSI 243

Query: 228 -----DCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDID 283
                D YPE L +++I++    F   W  V  F+D  T  KI  V   K ++ LL  ID
Sbjct: 244 QKIDNDNYPETLNRMFIINAGCGFRLLWNTVKSFLDPKTTAKI-HVLGNKYQTKLLEIID 303

BLAST of Cp4.1LG08g06350 vs. ExPASy Swiss-Prot
Match: P24280 (SEC14 cytosolic factor OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SEC14 PE=1 SV=3)

HSP 1 Score: 86.3 bits (212), Expect = 6.2e-16
Identity = 79/273 (28.94%), Postives = 124/273 (45.42%), Query Frame = 0

Query: 22  TEQEEE--EDEFKECLEEPNGSVTPVFEDSLESNEVEKQKVVIMRAFVEREDSSAKDVDD 81
           T+QE+E  E   + C  +         + + E    E +K++    F+ER       +DD
Sbjct: 3   TQQEKEFLESYPQNCPPDALPGTPGNLDSAQEKALAELRKLLEDAGFIER-------LDD 62

Query: 82  FMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASEISTNLAHNKLFMQ---GVD 141
             + RFLRAR  D++ A  MF     WRK    +  +            K + Q     D
Sbjct: 63  STLLRFLRARKFDVQLAKEMFENCEKWRKDYGTDTILQDFHYDEKPLIAKFYPQYYHKTD 122

Query: 142 KKGRPIV---VGYGNRHKQGNM---EEFIRYVIFALEKI-------CSRMPRGQEKFVC- 201
           K GRP+    +G  N H+   +   E  ++ +++  E +       CSR      +  C 
Sbjct: 123 KDGRPVYFEELGAVNLHEMNKVTSEERMLKNLVWEYESVVQYRLPACSRAAGHLVETSCT 182

Query: 202 IGDLQGWGYSNS-DIRGY-RASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 261
           I DL+G   S++  +  Y R +  I Q+ YPER+GK YI++ P+ F TA+++  PF+D  
Sbjct: 183 IMDLKGISISSAYSVMSYVREASYISQNYYPERMGKFYIINAPFGFSTAFRLFKPFLDPV 242

Query: 262 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGK 274
           T  KI F+     +  LL  I    LP  +GGK
Sbjct: 243 TVSKI-FILGSSYQKELLKQIPAENLPVKFGGK 267

BLAST of Cp4.1LG08g06350 vs. NCBI nr
Match: XP_023539819.1 (phosphatidylinositol transfer protein 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 563 bits (1450), Expect = 3.28e-202
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282

BLAST of Cp4.1LG08g06350 vs. NCBI nr
Match: KAG6597698.1 (Phosphatidylinositol transfer protein 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 561 bits (1446), Expect = 1.34e-201
Identity = 281/282 (99.65%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGN+EEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282

BLAST of Cp4.1LG08g06350 vs. NCBI nr
Match: KAG7029143.1 (Random slug protein 5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 560 bits (1443), Expect = 3.83e-201
Identity = 280/282 (99.29%), Postives = 282/282 (100.00%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGN+EEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKL+LVPIQDC
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLALVPIQDC 282

BLAST of Cp4.1LG08g06350 vs. NCBI nr
Match: XP_022932698.1 (phosphatidylinositol transfer protein 3 [Cucurbita moschata])

HSP 1 Score: 556 bits (1433), Expect = 1.28e-199
Identity = 279/281 (99.29%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKS IPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSVIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGN+EEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 281
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 281

BLAST of Cp4.1LG08g06350 vs. NCBI nr
Match: XP_022972040.1 (phosphatidylinositol transfer protein 3-like [Cucurbita maxima])

HSP 1 Score: 553 bits (1426), Expect = 1.50e-198
Identity = 276/282 (97.87%), Postives = 280/282 (99.29%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MA PLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MAIPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLD+EKASAMFLKYLSWRK+AIPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDVEKASAMFLKYLSWRKAAIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGN+EEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVP+IF TAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPFIFTTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282

BLAST of Cp4.1LG08g06350 vs. ExPASy TrEMBL
Match: A0A6J1F2H3 (phosphatidylinositol transfer protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111439167 PE=4 SV=1)

HSP 1 Score: 556 bits (1433), Expect = 6.21e-200
Identity = 279/281 (99.29%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKS IPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSVIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGN+EEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 281
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 281

BLAST of Cp4.1LG08g06350 vs. ExPASy TrEMBL
Match: A0A6J1I7E9 (phosphatidylinositol transfer protein 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470692 PE=4 SV=1)

HSP 1 Score: 553 bits (1426), Expect = 7.25e-199
Identity = 276/282 (97.87%), Postives = 280/282 (99.29%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MA PLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV
Sbjct: 1   MAIPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
           VIMRAFVEREDSSAKDVDDFMIRRFLRARDLD+EKASAMFLKYLSWRK+AIPNGFISASE
Sbjct: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDVEKASAMFLKYLSWRKAAIPNGFISASE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGN+EEFIRYVIFALEKICSRMPRGQEKF
Sbjct: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNIEEFIRYVIFALEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVP+IF TAWKMVYPFIDKN
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPFIFTTAWKMVYPFIDKN 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
           TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC
Sbjct: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282

BLAST of Cp4.1LG08g06350 vs. ExPASy TrEMBL
Match: A0A6J1J0A3 (patellin-4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480094 PE=4 SV=1)

HSP 1 Score: 498 bits (1283), Expect = 4.69e-177
Identity = 245/283 (86.57%), Postives = 264/283 (93.29%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWT-EQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQK 60
           MAT LSRSEERN T  EK  WT E+EEEE EFKEC+EEPNGS+TPV +DSLESNE++KQK
Sbjct: 1   MATQLSRSEERNATPKEKLEWTKEEEEEEKEFKECVEEPNGSLTPVIDDSLESNELDKQK 60

Query: 61  VVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISAS 120
           + IMRAFVE EDSSAKDVDDFM+RRFLRARDLDIEKASAMFLKYLSWR+SAIP GFIS S
Sbjct: 61  LAIMRAFVEGEDSSAKDVDDFMLRRFLRARDLDIEKASAMFLKYLSWRRSAIPGGFISVS 120

Query: 121 EISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEK 180
           E+STNLAHNKLFMQGVDKKGRPIVVG+GNRHKQGN+EEFIR+VIF LEK+CSR+PRGQEK
Sbjct: 121 ELSTNLAHNKLFMQGVDKKGRPIVVGFGNRHKQGNLEEFIRFVIFFLEKVCSRIPRGQEK 180

Query: 181 FVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDK 240
           FVCIGDLQGWGYSNSDIRGYRA+LSILQDCYPERLGKLY+VHVPYIFMTAWKMVYPFIDK
Sbjct: 181 FVCIGDLQGWGYSNSDIRGYRAALSILQDCYPERLGKLYLVHVPYIFMTAWKMVYPFIDK 240

Query: 241 NTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
            TK KI FVEDKKL+STLL DI+ESQLPD YGGKLSLVPIQDC
Sbjct: 241 KTKNKISFVEDKKLKSTLLDDINESQLPDAYGGKLSLVPIQDC 283

BLAST of Cp4.1LG08g06350 vs. ExPASy TrEMBL
Match: A0A6J1GUP1 (patellin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111457645 PE=4 SV=1)

HSP 1 Score: 495 bits (1275), Expect = 8.67e-176
Identity = 246/286 (86.01%), Postives = 264/286 (92.31%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDE----FKECLEEPNGSVTPVFEDSLESNEVE 60
           MA+ LSRS ERN T  EKS WTE+EEEE+E    FKEC+EEPNGS+TPV +DSLESNE+E
Sbjct: 1   MASQLSRSVERNATPKEKSEWTEEEEEEEEEEKEFKECVEEPNGSLTPVIDDSLESNELE 60

Query: 61  KQKVVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFI 120
           KQK+ IMRAFVE EDSSAKDVDDFM+RRFLRARDLDIEKASAMFLKYLSWR+SAIP+GFI
Sbjct: 61  KQKLAIMRAFVEGEDSSAKDVDDFMLRRFLRARDLDIEKASAMFLKYLSWRRSAIPDGFI 120

Query: 121 SASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRG 180
           S SE+STNLAHNKLFMQGVDKKG PIVVG+GNRHKQGN+EEFIR+VIFAL+KICSR+PRG
Sbjct: 121 SVSELSTNLAHNKLFMQGVDKKGHPIVVGFGNRHKQGNLEEFIRFVIFALDKICSRIPRG 180

Query: 181 QEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPF 240
           QEKFVCIGDLQGWGYSNSDIRGYRA+LSILQD YPERLGKLYIVHVPYIFMTAWKMVYPF
Sbjct: 181 QEKFVCIGDLQGWGYSNSDIRGYRAALSILQDYYPERLGKLYIVHVPYIFMTAWKMVYPF 240

Query: 241 IDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQDC 282
           IDK TK KI FVEDKKL STLL DIDESQLPD YGGKLSLVPIQDC
Sbjct: 241 IDKKTKNKISFVEDKKLNSTLLDDIDESQLPDAYGGKLSLVPIQDC 286

BLAST of Cp4.1LG08g06350 vs. ExPASy TrEMBL
Match: A0A6J1DXU4 (random slug protein 5 OS=Momordica charantia OX=3673 GN=LOC111025140 PE=4 SV=1)

HSP 1 Score: 494 bits (1272), Expect = 2.07e-175
Identity = 243/281 (86.48%), Postives = 261/281 (92.88%), Query Frame = 0

Query: 1   MATPLSRSEERNVTSMEKSAWTEQEEEEDEFKECLEEPNGSVTPVFEDSLESNEVEKQKV 60
           MATP S+  E   TSMEKS WTE +EEEDEF+EC+E+ NGSV+PV ED L+SNE+EK+KV
Sbjct: 1   MATPQSKIVENKATSMEKSKWTE-DEEEDEFEECVEDQNGSVSPVLEDGLDSNELEKRKV 60

Query: 61  VIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAIPNGFISASE 120
            +MRAFVERED SAKDVDDFMIRRFLRARDLDIEKASAMFLKYL+WR+SAIPNGFIS SE
Sbjct: 61  ALMRAFVEREDPSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLTWRRSAIPNGFISLSE 120

Query: 121 ISTNLAHNKLFMQGVDKKGRPIVVGYGNRHKQGNMEEFIRYVIFALEKICSRMPRGQEKF 180
           IS N+AHNKLFMQGVDKKGRPIVVG+G RHKQGNMEEFIRYVIF LEKICSRMPRGQEKF
Sbjct: 121 ISNNIAHNKLFMQGVDKKGRPIVVGFGGRHKQGNMEEFIRYVIFVLEKICSRMPRGQEKF 180

Query: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKN 240
           VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDK 
Sbjct: 181 VCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKK 240

Query: 241 TKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 281
           TKKKI FVEDKKLRSTLLSDIDESQ+PD+YGGKL L+PI+D
Sbjct: 241 TKKKISFVEDKKLRSTLLSDIDESQVPDIYGGKLQLIPIED 280

BLAST of Cp4.1LG08g06350 vs. TAIR 10
Match: AT1G01630.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 348.6 bits (893), Expect = 4.7e-96
Identity = 173/258 (67.05%), Postives = 202/258 (78.29%), Query Frame = 0

Query: 30  EFKECLEEPNGS----VTPVFEDSLESNEVEKQKVVIMRAFVEREDSSAKDVDDFMIRRF 89
           E KE  +EP  +      P+ ED     E+E+ KV IMRA  +R+D   K+VDD MIRRF
Sbjct: 2   ENKETKQEPAAAAEQKTVPLIED-----EIERSKVGIMRALCDRQDPETKEVDDLMIRRF 61

Query: 90  LRARDLDIEKASAMFLKYLSWRKSAIPNGFISASEISTNLAHNKLFMQGVDKKGRPIVVG 149
           LRARDLDIEKAS MFL YL+W++S +P G I  +EI+ +L+HNK+ MQG DK GRPI V 
Sbjct: 62  LRARDLDIEKASTMFLNYLTWKRSMLPKGHIPEAEIANDLSHNKMCMQGHDKMGRPIAVA 121

Query: 150 YGNRH--KQGNMEEFIRYVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASL 209
            GNRH   +GN +EF R+V++ LEKIC+RMPRGQEKFV IGDLQGWGYSN DIRGY A+L
Sbjct: 122 IGNRHNPSKGNPDEFKRFVVYTLEKICARMPRGQEKFVAIGDLQGWGYSNCDIRGYLAAL 181

Query: 210 SILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDE 269
           S LQDCYPERLGKLYIVH PYIFMTAWK++YPFID NTKKKI FVE+KKL  TLL DIDE
Sbjct: 182 STLQDCYPERLGKLYIVHAPYIFMTAWKVIYPFIDANTKKKIVFVENKKLTPTLLEDIDE 241

Query: 270 SQLPDVYGGKLSLVPIQD 282
           SQLPD+YGGKL LVPIQ+
Sbjct: 242 SQLPDIYGGKLPLVPIQE 254

BLAST of Cp4.1LG08g06350 vs. TAIR 10
Match: AT1G14820.3 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 146.0 bits (367), Expect = 4.7e-35
Identity = 85/234 (36.32%), Postives = 125/234 (53.42%), Query Frame = 0

Query: 54  EVEKQKVVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAI-P 113
           E ++  +  +R  VE+  SS +  D   + RFL AR +D  KA+ MF+ +  WR S + P
Sbjct: 3   ESQELALTQLRKSVEKLSSSTEGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPP 62

Query: 114 NGFISASEISTNLAHNKLFMQGVDKKGRPIVVGYGNRH-KQGNMEEFIRYVIFALEKICS 173
            GFI  SE+   L   K+ +QG  K G P+V+   ++H    +   F ++V++AL+K  +
Sbjct: 63  TGFIPESEVQDELEFRKVCLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIA 122

Query: 174 RMPRGQ----EKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFM 233
               G+    EK V + DL    Y N D RG       LQ  YPERL K YI+H+P  F+
Sbjct: 123 SGNNGKEVGGEKLVAVIDLANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFV 182

Query: 234 TAWKMVYPFIDKNTKKKIKFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 282
           T WK V  F++K T++KI  V D + +     +I    LP+ YGG+  L  IQD
Sbjct: 183 TVWKFVCRFLEKATQEKIVIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQD 236

BLAST of Cp4.1LG08g06350 vs. TAIR 10
Match: AT1G14820.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-34
Identity = 82/216 (37.96%), Postives = 116/216 (53.70%), Query Frame = 0

Query: 72  SSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAI-PNGFISASEISTNLAHNKL 131
           SS K  D   + RFL AR +D  KA+ MF+ +  WR S + P GFI  SE+   L   K+
Sbjct: 8   SSEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKV 67

Query: 132 FMQGVDKKGRPIVVGYGNRH-KQGNMEEFIRYVIFALEKICSRMPRGQ----EKFVCIGD 191
            +QG  K G P+V+   ++H    +   F ++V++AL+K  +    G+    EK V + D
Sbjct: 68  CLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVID 127

Query: 192 LQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKI 251
           L    Y N D RG       LQ  YPERL K YI+H+P  F+T WK V  F++K T++KI
Sbjct: 128 LANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKI 187

Query: 252 KFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 282
             V D + +     +I    LP+ YGG+  L  IQD
Sbjct: 188 VIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQD 223

BLAST of Cp4.1LG08g06350 vs. TAIR 10
Match: AT1G14820.1 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 144.1 bits (362), Expect = 1.8e-34
Identity = 82/216 (37.96%), Postives = 116/216 (53.70%), Query Frame = 0

Query: 72  SSAKDVDDFMIRRFLRARDLDIEKASAMFLKYLSWRKSAI-PNGFISASEISTNLAHNKL 131
           SS K  D   + RFL AR +D  KA+ MF+ +  WR S + P GFI  SE+   L   K+
Sbjct: 8   SSEKGYDKPTLMRFLVARSMDPVKAAKMFVDWQKWRASMVPPTGFIPESEVQDELEFRKV 67

Query: 132 FMQGVDKKGRPIVVGYGNRH-KQGNMEEFIRYVIFALEKICSRMPRGQ----EKFVCIGD 191
            +QG  K G P+V+   ++H    +   F ++V++AL+K  +    G+    EK V + D
Sbjct: 68  CLQGPTKSGHPLVLVITSKHFASKDPANFKKFVVYALDKTIASGNNGKEVGGEKLVAVID 127

Query: 192 LQGWGYSNSDIRGYRASLSILQDCYPERLGKLYIVHVPYIFMTAWKMVYPFIDKNTKKKI 251
           L    Y N D RG       LQ  YPERL K YI+H+P  F+T WK V  F++K T++KI
Sbjct: 128 LANITYKNLDARGLITGFQFLQSYYPERLAKCYILHMPGFFVTVWKFVCRFLEKATQEKI 187

Query: 252 KFVEDKKLRSTLLSDIDESQLPDVYGGKLSLVPIQD 282
             V D + +     +I    LP+ YGG+  L  IQD
Sbjct: 188 VIVTDGEEQRKFEEEIGADALPEEYGGRAKLTAIQD 223

BLAST of Cp4.1LG08g06350 vs. TAIR 10
Match: AT1G22180.2 (Sec14p-like phosphatidylinositol transfer family protein )

HSP 1 Score: 105.1 bits (261), Expect = 9.1e-23
Identity = 74/242 (30.58%), Postives = 119/242 (49.17%), Query Frame = 0

Query: 37  EPNGSVTPVFEDSLESNEVEKQKVVIMRAFVEREDSSAKDVDDFMIRRFLRARDLDIEKA 96
           + N S +  FE SL   E   +   +        + S++   D  I R+L AR+  ++KA
Sbjct: 4   DSNKSSSNGFEKSLTPEEYLNKINEVRTLLGPLTEKSSEFCSDAAITRYLAARNGHVKKA 63

Query: 97  SAMFLKYLSWRKSAIPNGFISASEISTNLAHNKLFMQG-VDKKGRPIVVGYGNRHKQGNM 156
           + M  + L WR    P   I   EI+      K++     DK GR ++V   +     + 
Sbjct: 64  TKMLKETLKWRAQYKPEE-IRWEEIAREAETGKIYRANCTDKYGRTVLVMRPSCQNTKSY 123

Query: 157 EEFIRYVIFALEKICSRMPRGQEKFVCIGDLQGWGYSNSDIRGYRASLSILQDCYPERLG 216
           +  IR +++ +E     +P  QE+ V + D  G+  S+  ++  R +  +LQ+ YPERLG
Sbjct: 124 KGQIRILVYCMENAILNLPDNQEQMVWLIDFHGFNMSHISLKVSRETAHVLQEHYPERLG 183

Query: 217 KLYIVHVPYIFMTAWKMVYPFIDKNTKKKIKFV-EDKKLRSTLLSDI-DESQLPDVYGGK 276
              + + P IF + +KMV PF++  T  K+KFV  D  L + LL D+ D  QL   +GGK
Sbjct: 184 LAIVYNPPKIFESFYKMVKPFLEPKTSNKVKFVYSDDNLSNKLLEDLFDMEQLEVAFGGK 243