Cp4.1LG08g05780 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG08g05780
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionReticulon-like protein
LocationCp4.1LG08: 701846 .. 709064 (+)
RNA-Seq ExpressionCp4.1LG08g05780
SyntenyCp4.1LG08g05780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTATGAAATTTCCGCGCCAACTACTTCTCTCCAGCGTCGAATTCCAACCCTAACAACGACCTCAATGCATTTTCTTCTCTGTCTCATTCTCAATTCTCTTATTTTTCTCTGATCTTTCCTCCTATCGATCGGAAATGGAGGTCGACGATCGCTTTGTGGAATTGAATACCAGGACCTGTGTGGTGTTGGGCGGTCGTGGATTTCTCGGCCGATCTCTCGTTCTCCGCCTCTTGAAACTCGCCAAGTGGAATGTTCGTATCGCTGATTCTGTTGAATCGCTTGAACTCGATCCCTCTGAGCAGGATTCTCTCCTCTCTGACGCGCTTGTCGCTGGCCGGGCTTCGTATTTCCATGTCGATGTTCGCAATAAGGATCAGATCGTTGCAGGTGTTTCTTTCTCTCTCTCTTTGGTTGATTGTGAAACGTGGAGAAATGTTGCTTTGGTTGCATTTGGTTTTGAGTGGATGGAGCTAATTGAAGTGGTTTTGATTTTTTTTTTCTTTGAAATGCGAAGATTTTGAGATGTTTTCCCTGGCTTTGGAGCTGGTGGAATTTTAGAGGATTCGGATTGGTTTATTGTACACATGCTTTTTTGGAGTTTGAGTTAACTTTATCTTTTTGGAAATAACGATGGATAGAATCAGAAGATGATCGGACTGTTGAGACAGTTTCCATGAACTGCTGTTGAAGTCCGAAGAATTATTTGATTTGTTAAAAGGTTGTTAAGAGTCGAACGAAGCCTGCAACTCTAGGTTCTTGTTTCTTTCGTGGTGCGCATGACTTTTTCTTTTGTTTTTTTATTAATTTTTCAATTGTGTTTGTAAGGGATTTCGTTTGGAATATTCTCTCTGTTGAATCATAATGTTCGATTGAGAAACTTCTGTTATCTATGAATTTTGTGGAAATTCTCAACTGCATCATAATAAATAAATGAATAAATAAAATGGAATGTCTTCGATGGAGAAAAAATTTTCAGCTATCAATAGCGATGGGAGGTTTCCTGTAAAATTTTCATTTATTGCCATTCTTCACTGTTATTGCTTCCGTCTCTGACGCCTATTTTCTATCCATAAATATGTCGATTACTTAATTAGGTACCAGCTACTTGTATGACAGAGTGATTTTTTTTCCTTTTGCAGCTCTCAGTGGTTCTTCTGTTGTGTTTTATATGGACTATGAAGATTCACATTCAAATGATATTTATCTCTCATACATGTTTATAGTTCAAGGTATAGAAAGCCGTTAACCATTTGAAAGAACCCTTGTTATTCCGAACATATCCCACTTCAAAGAAAGATGTAATTGCCAGCATCTTGATTTCTCATTTAGGTGCCAAAAATGTCATTAATGCTTGTCGAGACTGCAAAGTAAGACGGCTAATATTCAACAGTTCAGCTGATGTAATTTTTGATGGTTCACGTGATATACAAAATGGAGAGGAGCCATTGGCATACCCACGGAAAGTATGTTGCTATCTGAACTATTTCTCAGTCATCTATTTTGGACATCCCTAACATTCTCTTCTGCTAATCATTTCCTCATCAAGTGAGTACTATGTACAGTTTGAGGACATGTTAAGTGACCTAAAGGCTCAAGCTGAAGCACTCATCCTGTTTGCCAACGATATAGATGGACTTCTAACATGTGCACTTCGCCCAAGCAATGTTTTTGGGCCAGAAGACACACGACTAGTTCCTTTCGTGGTGAATCAGGCAAAATCTGGTTTGGCTAAGGTTTGCAAAATTATTTATGTTTTACTCCATGCTTTGTTTTAGTGCTGCATGTAAACCTCTGTAAAATCCATAAAATCTTGTGGATTATTTGTGCTTTACCATTTAAATGAGCGTAAGCATAAGAACCATAGACCTAGTAGAAATTGGTGCCATGATACAATTCATTGTAGAAAGGTCATATATCTAATAATTAATTGAGAATAAATAATGAATTGAGAAAAAATGGAATAGAGTTAATAGAATAGCTATAGGATCTTTTTAATATTGAAGTACTTGATGAACTCGTGTTTTCTTTTCTCAAAGTAAACTTAATGCATTGAGAATGCATGCCTGTTAGTGTGATCTATACAAGATGTACATTGTCTACTCTTATTGAAGTAACAGAGTTTCTCATTTCAGTTCATTATAGGGAATGGCGAAAACCTGTCTGACTTCACATTTGTTGAGAATGTGACCCATGCCCATATCTGTGCAGAGGAAGCACTAGATTTTCGAATGGTGTCTGTGGCTGGAAAGGTATCACGTTTAAAAGATTGATACATGCCTTTTCTAATAATTTTAGTGATAGGGTTCATAAGAAATCATTTCCTTTTTATCATTATGTAGGCATTTTTCATCACCAATTTTAAGCCCATGAAGTTTTGGGAATTTATCTCTCTCATTTTGGGAGGCTTGGGTTACCGAAGGTACAGAATTACAGTCCATGAACTTTTAGACTTTGTTGTTCTGTCGATCCCTTTCCTAATTCTTTTATGCTTGCGTTTTCATATATTTGATGAGGATACTAAGGCTAGATAATTTTTAGTTCTCATTCTCTTTCGTTTGTAATTCTATCTTGCTATGTGTTCTGGCAGGCCATCCGTAAAACTTCCCTTTAAGATGGTTTGGAATGTTCTCTTGTTTTTGAAATGGATACATGAAAAGTTTGGATTCAGTAGATATAATCATTCCACTTGGGCTCGTTACATTCAGCTAGCCACGTGCACTAGAACCTTTAACTGTGTTGCTGCACATAAACAACTTGGGTACTCTCCTATAGTCTCCATGGAAGTAAGTTGTAATGAAAATATGTATCAGTTTCTCTTCCACTCAATATTTTTATTTAAATTGTTTTCTGGAAAATTGTCTCCTGTTTTTCTCACTTGTCTTTGTTTACTATCCTATCTTTGATTAATTTATTGATACTTAAGACAGGATACATATGATTATGCTGATTGGGATTTTTTGGTTTAGTCCTTTGGAAAAAAGGATTTGGTTGTAAATGAAGAACTTGAATTTTAAATTGTCTTGGGCGACCTTTGGGGGTTATGAGGTTTACCAACTCCCCTGTTGTATTTGGGTCTCCCTCAAGGGAGTATACCGAAGCTTTATCCTTTGTGCAATTCAGTGGTAGAGAAAGTTAAAGATGGTTCCCGTCCTCCAAAGGGGTGATAAAAATCAAAGCTCAGCAGAATACTTTAACCCAGGGAAAATTGACATCCAGGTGTTACAAAATTAAAATCCGTTTAACAAACATGGATAGTGATCATCCATGCAAAAAGCCCATATCATGTTCCTTGAAACAGACAGGCTATTATTGTTGTTTCTAGAGAATAAAGGATTAACTGGCTAAAAGAAACTCATCTTGGATTCTCAATCTTATTGACAAGAGTGCATGCCTTAGCCTCTCTTACTGTTAATATCTTATAGAAAGTTAGAATTTTCATAAAGGTAAAAGGCTTAGGAGCACGAATATAAATACGAAACATGGATACGACATGGGACACTTTAACACTTTATATTTAAAAATCTAGGGTATAAACACGACAATGACATGTTTATGAATAATAAGATTATTGAAAGTTTAAGAAATTAGAGCTTTTATTAGGGAGTAGGGAGAGAGGCCCCAATATCTAATGTGGCTTTCTTATTTTCTTCCTAATAAAATTTAAAATAATAAAATTTTAAAAACTTAAAATCCGGCTTCAAGCACTGAGGCGTGTTTCATAAGGGAGCAGGGTGCCTTTTGCCTGGGTGCTTTTCAAAGTGCTGCTACTTCTGGACATGTAGTTCATGGAATTCATGCTTTTATTTTCCTCTTGAACTTTTCTGACACCCGAAAGCCACTTTCATGGTATCTTCCCAAATAAGGTGGTAACAATTGCATTATGGAGCTAGGTACTAGCTGAATTTTTGGATTTGTTAGAGAAGCTAACGTAGATGATGCAGCTTACAAATCTTCTGCAACTTCTGTTTGATTGGAACAGTCGCACCTTTGTTTGAAGCACTGATAGCAGTACAGTTACAACTTTTTGTTCTCCATTTCTTTTTCTTTACAGGAAGGCATCACACTAACCATTGAATCATTTTCACATTTGCGCAAAGAATTATCTCTTGCAAGTCTCACTGAATTTACCGAAGAATCAAAAGCCGATAAGCTGCTGGGTGGTGGAAGAGGTTCAAACTTTTTCTCATTTAATGTATTTAGCATCATTTTCTATATTAATAATGCTTAGACTACTTCAATCGTCATTAATTGAATTTCCTGATTTCAACTTATCGACTTTGTGTCTTCTAGATGATTGTATTTTTTGTATTTTATATGTAACTTGAATGGTTGTTGCCAATTGACAAAATAAATGCATGAACATCACCTCATTCAATTTGATCATTATGTAGTTAACTAGAACCCCCAGCGGCTCAACTGGGACTGACTGGGAGCATGCTTGATATACAATATCCTTTATATGTCATTTTAAATCCTCTACAAATAAAGGTTTGGTTTCTTGTATTGAACACTTTTGTTAATAAATTCCTGTGGGTTATTATTTTTGTTTTAGAAAATTCTCTCTTTTGTTATTTAGGCTACTACACGGATATTTGGCCTGTGAGTGGAATTCAAATCACAGATTTCATTTGTAATTGCATGTGTTATAAATTGGAAAGCCTTTTTGTAGTATTCCTCTTTGGGAGGGAGTTTCCTCTATCCGTCCTTCAGGGTATGGTTTTAATATACTCTTACACATGTCTAACAGAGGAGAAGAGAAGAAGAAAAGGAAAAAAAGAAGAAAGCTTTCGCATATTGGGGGAAGTTGGTTGTTTTATGTAATATTTTGGGGTGCTTTGTGTGTCTGGTTTTCTCTTCTATTGATGGGTTCTTTTTCTTCTTTTTTTTACTTTTCTATTTTGCTTGTACTCGTACTCCTTTCATTTTATTGATGAGAAGTCTTGTTTCTATGCTAGAAAATACTCTATGGAGAAGAATCTCATTTACTGATGAATGTTGAAATTCATCTGTATACCATTTCAAATTTATAATTGTTCTTCCTTATTGCTGCATTATTTGGTTTACCTCCGTAACATAAGGTAAATCTGTACTTGGAGAATGACATTGGATTCTTTCTGCAGTTGCCGATATATTGTTATGGCGAGAAGAGAAGAAGTCATTCACGTGTTTTCTTGCTTCGTTTCTATTGTTTTATTGGTTTTTTCTACGTGGAGGAACTTTTATATCATCGGTGGCTCAGCTTCTGTTGTGTATCGTTGTCGTCCTTTATGGATATGGCTTCATACCAACAAACATGTAAGTATGCTCATGAAGAGCTTCTAGAAATTACAATTCTGAATCTTTTATGACTTTATGATTACTTTCTTTATCCTGGAAGTGGAAGTGAGATATTTCACATCCAAAATTCTAGGATCCTTGATTTATTTTTCTGCAATTAGTTTTTCCCATGATTATTGTTTTCTCGTGTAAGTGCATGATCATGACGAGTCCTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTGTTAATCATCACTATTTTAATTTTAGTTGAACTGATGCTGAGATTTTACCATCAGATATGGGTTTCCTGTCCAGAAATTATCTGCGACTATGTTTGGACCATCTGATACAGTAGTCAGAGATTCGATAATGGGCATAGCATGCTTGTGGAATAGAGGTGTACATAACGCAAGATCATTGGCCCGAGGAGAGGATTGGAATTATTTTTTGAAGGTATGACTTCGACTCTCGTACTTTGTTTGTAGAAGACGCTGCTGCGCTGTTTGTGAAATTTTGTATTTCATGAAGTTTTGCGTGCACTGTCCCAGTATTTTCTTTAAGTGCAAACCAAGAGACCCAATAGGCACGGTGACAAAGAAACCAAATCTCTTTTTGTTGTTTATGAAATACATAATGTGTGGAATAAAAACAGAGGAAAATATAGCGTTAACTTCCAGGGTTATGTCATACGTTGTTGAATTTTAAAAGGTCTTTAATAGGTTTAATAGGGCTTCAAGTGATTGGTCCAAATCTATTCAACATTCTTTTATATTTTATGCATAATAAAACTATTTCAATTTTTTGTTTAACAGATACATGAATTTTCAAATACTTCAAGTCTTAGAAATATCAAAATTTTGATCATATAATTAAGCACGATCGAAATTTTGAGCTTTTTACTGCTTCAAATTCATCTTAAGCTAATAGAATAGTTGGTTCTAAATGGTTCTACTTACCGTTTTGTGTTGTATATTTCACATAATTAAACGTTTCACTGATTGTACAGGCCGTAACATTTCTTTACGTCCTCAAGCTGTTTCTCTCACGATCCTTGACTTTGCTGATAGGCATGGGTAAGTTACAACTAAGTTTATTCCATAACACCTGGCAGTGCTTATTCCTTCTGACTTAGTTCAATAACTAATGGTAATAACATTGTAGGACTGGTGTTTGCATTCACTGCATTCTTTGTGTATGAGCAATACGAAATCGAGATTGATGGATTTGCTAAGTTCCTTTTCATCAGCATCATGGAATTAAAGAGTGTACTTGCAAGGCATTTACCACCTCCATTGATGTCATTTCTCTGGTGTGATGAATTATCACATCAACCTAAAGAAGTTGCAATGTCAAAGCGTTAGAGCATAATCCCAGAACGTTCAAGGATTGGAAGATTGTTCGAGTGGATCTCCTTCCGATCTATCCGTTAGCTTGCTCAGGTTTTTACCAGAATATATAACGTCTTTCCCCCCAGGAAAAGCGAAATGGAGCTAATCTATCTTATTAGGAAACTCACCCAGGTTCTTATCTTCTCACAACATTTTATACCATCTCTGTGCAATAAAACGTTGAAATGTGATGCGTAGTGTAGCAGGCGCGACGGAGATGTTGATGAATATTTGGGTTGAGTCTGGCTCTTCCCTCCTTTTCTATCAACAACATTACTTGTACTATTGGTAGCCAGCAGCTAGGGCAGTTTTTCAAACGTGGGGCTGCAATTTCATTTTCCTTGAAACACCGTACAATCTTGAAGATGATACAGATTCCCTCTCCCCTTGAATGCAATGCAATTTCTTAAGCCTTTTACTTTGTGATTTAAAGTTACTTTGGTTTCTAAATTTTACATTTTGTTCTATTTTGGTCTCAGAACTTTTTAGGAACTTTCAAAATACTATCTATTAATTAGAT

mRNA sequence

GTATGAAATTTCCGCGCCAACTACTTCTCTCCAGCGTCGACGATCGCTTTGTGGAATTGAATACCAGGACCTGTGTGGTGTTGGGCGGTCGTGGATTTCTCGGCCGATCTCTCGTTCTCCGCCTCTTGAAACTCGCCAAGTGGAATGTTCGTATCGCTGATTCTGTTGAATCGCTTGAACTCGATCCCTCTGAGCAGGATTCTCTCCTCTCTGACGCGCTTGTCGCTGGCCGGGCTTCGTATTTCCATGTCGATGTTCGCAATAAGGATCAGATCGTTGCAGCTCTCAGTGGTTCTTCTGTTGTGTTTTATATGGACTATGAAGATTCACATTCAAATGATATTTATCTCTCATACATGTTTATAGTTCAAGGTGCCAAAAATGTCATTAATGCTTGTCGAGACTGCAAAGTAAGACGGCTAATATTCAACAGTTCAGCTGATGTAATTTTTGATGGTTCACGTGATATACAAAATGGAGAGGAGCCATTGGCATACCCACGGAAATTTGAGGACATGTTAAGTGACCTAAAGGCTCAAGCTGAAGCACTCATCCTGTTTGCCAACGATATAGATGGACTTCTAACATGTGCACTTCGCCCAAGCAATGTTTTTGGGCCAGAAGACACACGACTAGTTCCTTTCGTGGTGAATCAGGCAAAATCTGGTTTGGCTAAGTTCATTATAGGGAATGGCGAAAACCTGTCTGACTTCACATTTGTTGAGAATGTGACCCATGCCCATATCTGTGCAGAGGAAGCACTAGATTTTCGAATGGTGTCTGTGGCTGGAAAGGCATTTTTCATCACCAATTTTAAGCCCATGAAGTTTTGGGAATTTATCTCTCTCATTTTGGGAGGCTTGGGTTACCGAAGGCCATCCGTAAAACTTCCCTTTAAGATGGTTTGGAATGTTCTCTTGTTTTTGAAATGGATACATGAAAAGTTTGGATTCAGTAGATATAATCATTCCACTTGGGCTCGTTACATTCAGCTAGCCACGTGCACTAGAACCTTTAACTGTGTTGCTGCACATAAACAACTTGGGTACTCTCCTATAGTCTCCATGGAAGAAGGCATCACACTAACCATTGAATCATTTTCACATTTGCGCAAAGAATTATCTCTTGCAAGTCTCACTGAATTTACCGAAGAATCAAAAGCCGATAAGCTGCTGGGTGGTGGAAGAGTTGCCGATATATTGTTATGGCGAGAAGAGAAGAAGTCATTCACGTGTTTTCTTGCTTCGTTTCTATTGTTTTATTGGTTTTTTCTACGTGGAGGAACTTTTATATCATCGGTGGCTCAGCTTCTGTTGTGTATCGTTGTCGTCCTTTATGGATATGGCTTCATACCAACAAACATATATGGGTTTCCTGTCCAGAAATTATCTGCGACTATGTTTGGACCATCTGATACAGTAGTCAGAGATTCGATAATGGGCATAGCATGCTTGTGGAATAGAGGTGTACATAACGCAAGATCATTGGCCCGAGGAGAGGATTGGAATTATTTTTTGAAGGCCGTAACATTTCTTTACGTCCTCAAGCTGTTTCTCTCACGATCCTTGACTTTGCTGATAGGCATGGGACTGGTGTTTGCATTCACTGCATTCTTTGTGTATGAGCAATACGAAATCGAGATTGATGGATTTGCTAAGTTCCTTTTCATCAGCATCATGGAATTAAAGAGTGTACTTGCAAGGCATTTACCACCTCCATTGATGTCATTTCTCTGGTGTGATGAATTATCACATCAACCTAAAGAAGTTGCAATGTCAAAGCGTTAGAGCATAATCCCAGAACGTTCAAGGATTGGAAGATTGTTCGAGTGGATCTCCTTCCGATCTATCCGTTAGCTTGCTCAGGTTTTTACCAGAATATATAACGTCTTTCCCCCCAGGAAAAGCGAAATGGAGCTAATCTATCTTATTAGGAAACTCACCCAGGTTCTTATCTTCTCACAACATTTTATACCATCTCTGTGCAATAAAACGTTGAAATGTGATGCGTAGTGTAGCAGGCGCGACGGAGATGTTGATGAATATTTGGGTTGAGTCTGGCTCTTCCCTCCTTTTCTATCAACAACATTACTTGTACTATTGGTAGCCAGCAGCTAGGGCAGTTTTTCAAACGTGGGGCTGCAATTTCATTTTCCTTGAAACACCGTACAATCTTGAAGATGATACAGATTCCCTCTCCCCTTGAATGCAATGCAATTTCTTAAGCCTTTTACTTTGTGATTTAAAGTTACTTTGGTTTCTAAATTTTACATTTTGTTCTATTTTGGTCTCAGAACTTTTTAGGAACTTTCAAAATACTATCTATTAATTAGAT

Coding sequence (CDS)

ATGAAATTTCCGCGCCAACTACTTCTCTCCAGCGTCGACGATCGCTTTGTGGAATTGAATACCAGGACCTGTGTGGTGTTGGGCGGTCGTGGATTTCTCGGCCGATCTCTCGTTCTCCGCCTCTTGAAACTCGCCAAGTGGAATGTTCGTATCGCTGATTCTGTTGAATCGCTTGAACTCGATCCCTCTGAGCAGGATTCTCTCCTCTCTGACGCGCTTGTCGCTGGCCGGGCTTCGTATTTCCATGTCGATGTTCGCAATAAGGATCAGATCGTTGCAGCTCTCAGTGGTTCTTCTGTTGTGTTTTATATGGACTATGAAGATTCACATTCAAATGATATTTATCTCTCATACATGTTTATAGTTCAAGGTGCCAAAAATGTCATTAATGCTTGTCGAGACTGCAAAGTAAGACGGCTAATATTCAACAGTTCAGCTGATGTAATTTTTGATGGTTCACGTGATATACAAAATGGAGAGGAGCCATTGGCATACCCACGGAAATTTGAGGACATGTTAAGTGACCTAAAGGCTCAAGCTGAAGCACTCATCCTGTTTGCCAACGATATAGATGGACTTCTAACATGTGCACTTCGCCCAAGCAATGTTTTTGGGCCAGAAGACACACGACTAGTTCCTTTCGTGGTGAATCAGGCAAAATCTGGTTTGGCTAAGTTCATTATAGGGAATGGCGAAAACCTGTCTGACTTCACATTTGTTGAGAATGTGACCCATGCCCATATCTGTGCAGAGGAAGCACTAGATTTTCGAATGGTGTCTGTGGCTGGAAAGGCATTTTTCATCACCAATTTTAAGCCCATGAAGTTTTGGGAATTTATCTCTCTCATTTTGGGAGGCTTGGGTTACCGAAGGCCATCCGTAAAACTTCCCTTTAAGATGGTTTGGAATGTTCTCTTGTTTTTGAAATGGATACATGAAAAGTTTGGATTCAGTAGATATAATCATTCCACTTGGGCTCGTTACATTCAGCTAGCCACGTGCACTAGAACCTTTAACTGTGTTGCTGCACATAAACAACTTGGGTACTCTCCTATAGTCTCCATGGAAGAAGGCATCACACTAACCATTGAATCATTTTCACATTTGCGCAAAGAATTATCTCTTGCAAGTCTCACTGAATTTACCGAAGAATCAAAAGCCGATAAGCTGCTGGGTGGTGGAAGAGTTGCCGATATATTGTTATGGCGAGAAGAGAAGAAGTCATTCACGTGTTTTCTTGCTTCGTTTCTATTGTTTTATTGGTTTTTTCTACGTGGAGGAACTTTTATATCATCGGTGGCTCAGCTTCTGTTGTGTATCGTTGTCGTCCTTTATGGATATGGCTTCATACCAACAAACATATATGGGTTTCCTGTCCAGAAATTATCTGCGACTATGTTTGGACCATCTGATACAGTAGTCAGAGATTCGATAATGGGCATAGCATGCTTGTGGAATAGAGGTGTACATAACGCAAGATCATTGGCCCGAGGAGAGGATTGGAATTATTTTTTGAAGGCCGTAACATTTCTTTACGTCCTCAAGCTGTTTCTCTCACGATCCTTGACTTTGCTGATAGGCATGGGACTGGTGTTTGCATTCACTGCATTCTTTGTGTATGAGCAATACGAAATCGAGATTGATGGATTTGCTAAGTTCCTTTTCATCAGCATCATGGAATTAAAGAGTGTACTTGCAAGGCATTTACCACCTCCATTGATGTCATTTCTCTGGTGTGATGAATTATCACATCAACCTAAAGAAGTTGCAATGTCAAAGCGTTAG

Protein sequence

MKFPRQLLLSSVDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR
Homology
BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match: A9X4U2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D3 PE=2 SV=2)

HSP 1 Score: 568.5 bits (1464), Expect = 8.6e-161
Identity = 299/544 (54.96%), Postives = 378/544 (69.49%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           +TCVVLGGRGF+GRSLV RLL+L  W VR+ADS  +L LD  E DSLL DAL +GRASY 
Sbjct: 13  KTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRASYH 72

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
            VDVR+K QIV    GS VVFYM   D  S+D +  Y  IVQG +NVI+ACR+  VR+LI
Sbjct: 73  CVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVRKLI 132

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           +NS+ADV+FDGS+ I++G+E L  P KF+ ML+D KAQAEALI  AN+ DGLLTCALR S
Sbjct: 133 YNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCALRSS 192

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVSV 261
            VFGP DT  VPF+VN AKSG AKFI+G+GEN+SDFT+ ENV+HAHICA +ALD +M  V
Sbjct: 193 IVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQMEFV 252

Query: 262 AGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRYN 321
           AGK FFITN KP++FW+F+S I+ GLGY RPS+KLP ++V  V   LKW HEK G    N
Sbjct: 253 AGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHEKEGLGS-N 312

Query: 322 HSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEF 381
           + T  +Y  LA+ TRTFNC AA K LGY+P+V++E+GI  T++ FS       L    + 
Sbjct: 313 YDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-----DLEKSDDT 372

Query: 382 TEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIV 441
             +S AD+LLG G+VADILLWR EKK+F  FL   L +YWFF  G TF SS AQLL    
Sbjct: 373 IIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLLFIFA 432

Query: 442 VVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDW 501
           V LYG  F+P+ I+GF V K+    F  S++ VRD    I  +WN+GV + +SL+ G DW
Sbjct: 433 VALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSSGGDW 492

Query: 502 NYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMEL 561
             F K    LY+LKL +SRSL   +   + F+FT FF+YEQYE+E+   A+     +  +
Sbjct: 493 IKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIFVECLTFI 548

Query: 562 KSVL 566
           K ++
Sbjct: 553 KRMV 548

BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match: Q67ZE1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D2 PE=1 SV=2)

HSP 1 Score: 421.0 bits (1081), Expect = 2.2e-116
Identity = 232/551 (42.11%), Postives = 331/551 (60.07%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           R CVV GGRGF  R LV  L++   + VRIAD   ++ LDP E + +L + L +GR  Y 
Sbjct: 10  RWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYI 69

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+R+K Q+V A  G+ VVF+M   DS  N+  L Y   VQG +NVI+AC D  V+RLI
Sbjct: 70  SADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLI 129

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           + SS  V+FDG   I NG E +AYP K  D  S  KA+ E LI+ AN  +GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPS 189

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
           ++FGP D  LVP +V  A++G +KFIIG+G NL DFT+VENV HAH+CAE AL       
Sbjct: 190 SIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVS 249

Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
              AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P  ++  +   ++  ++  G +
Sbjct: 250 TKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPY 309

Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
                      ++L +C+RTF+   A  +LGY+P+V ++EGI  TI+SFSH    L+  S
Sbjct: 310 GMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH----LTAGS 369

Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLL 441
            ++    SKA ++LGGG+VAD LLW++ K++         ++Y F   G T ++++++ L
Sbjct: 370 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 429

Query: 442 LCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLAR 501
           L   V L+ +G +P  I+G+ V+K+ A+ F  S     D  + +   WN  V   RSL +
Sbjct: 430 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 489

Query: 502 GEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFIS 561
           G DW++F K V  L  L L  + SL  +  +GL  AF AF VYE+ E EID         
Sbjct: 490 GNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSI------- 549

Query: 562 IMELKSVLARH 569
           ++  KS   +H
Sbjct: 550 VVSFKSFACKH 549

BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match: Q9FX01 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D1 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 2.6e-85
Identity = 178/448 (39.73%), Postives = 263/448 (58.71%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           R CVV GGRGF  R LV  L++   ++VRIAD   ++ L+P E+  +L +A+ +GR  Y 
Sbjct: 10  RWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYV 69

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+RNK Q+V    G+ VVF+M   DS  N+  L Y   VQG  NVI+AC +  V+RLI
Sbjct: 70  SADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLI 129

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           + SS  V+FDG     N +E L YP K  D  S  KA+ EALIL AN   GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPS 189

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
           ++FGP D  +VP +V  A++G +KFIIG+G N  DFT+VENV HAH+CAE AL       
Sbjct: 190 SIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVC 249

Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
              AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P  ++  +   ++  ++  G +
Sbjct: 250 AKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPY 309

Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
                      ++L +C RTF+   A  +LGYSP+V ++EGI  TI+SFSHL+ +     
Sbjct: 310 GMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ----- 369

Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGT---FISSVA 441
                 + K +       V + + W+  K++    +    L++ F    G+    I++V+
Sbjct: 370 -----NQPKTE-------VTETIQWK--KQTLIAIVILITLYHNFVATTGSSSVIITAVS 429

Query: 442 QLLLCIVVVLYGYGFIPTNIYGFPVQKL 463
           ++LL   + ++  G +P  +  F  +K+
Sbjct: 430 KVLLVSSIFMFINGILPEKMKVFGSKKI 438

BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match: Q5PPL3 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus OX=10116 GN=Nsdhl PE=2 SV=1)

HSP 1 Score: 225.7 bits (574), Expect = 1.4e-57
Identity = 133/353 (37.68%), Postives = 195/353 (55.24%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           + C V+GG GFLG+ +V +LL    + V + D  +  + +P              R  +F
Sbjct: 27  KKCTVIGGSGFLGQHMVEQLLSRG-YAVNVFDVRQGFD-NP--------------RVQFF 86

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+ N+  +  AL G S VF+     S+SN+  L Y     G K VI  C++  V++LI
Sbjct: 87  IGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQKLI 146

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFAND-IDGLLTCALRP 201
             SSA V+F+G  DI+NG E L Y  K  D  ++ K   E  +L AND     LT A+RP
Sbjct: 147 LTSSASVVFEGV-DIKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAAIRP 206

Query: 202 SNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVS 261
             +FGP D +LVP +++ A+ G  KF+IGNG+NL DFTFVENV H HI A E L  R   
Sbjct: 207 HGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLS-RDAG 266

Query: 262 VAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRY 321
           + GKAF ITN +P+ FW F+S IL GL Y  P   +P+++ + +   L  +         
Sbjct: 267 LGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMVLSPLIQ 326

Query: 322 NHSTWARY-IQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKE 373
             +T+  + + LA     ++C  A K +GY P+V+M++ +  T++SF HLRK+
Sbjct: 327 IQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDDAVERTVQSFHHLRKD 361

BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match: Q9R1J0 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=10090 GN=Nsdhl PE=1 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 4.0e-57
Identity = 135/353 (38.24%), Postives = 192/353 (54.39%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           + C V+GG GFLG+ +V +LL+   + V + D  +  + +P              R  +F
Sbjct: 27  KKCTVIGGSGFLGQHMVEQLLERG-YTVNVFDIHQGFD-NP--------------RVQFF 86

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+ N+  +  AL G S VF+      +SN+  L Y     G K VI  CR+  V++LI
Sbjct: 87  IGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQKLI 146

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFAND-IDGLLTCALRP 201
             SSA V+F+G  DI+NG E L Y  K  D  ++ K   E  +L AND     LT A+RP
Sbjct: 147 LTSSASVVFEGV-DIKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAAIRP 206

Query: 202 SNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVS 261
             +FGP D +LVP +++ A+ G  KF+IGNGENL DFTFVENV H HI A E L  +  +
Sbjct: 207 HGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLS-QDAA 266

Query: 262 VAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRY 321
           + GKAF ITN +P+ FW F+S IL GL Y  P   +P+ M + +   L  +         
Sbjct: 267 LGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMVVSPLIQ 326

Query: 322 NHSTWARY-IQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKE 373
              T+    + LA     ++C  A K  GY P+V+M+E +  T++SF HLRK+
Sbjct: 327 IQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDEAVERTVQSFHHLRKD 361

BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match: XP_023540745.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1154 bits (2986), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD
Sbjct: 3   VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
           HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 585

BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match: KAG6597621.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029062.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1142 bits (2955), Expect = 0.0
Identity = 577/583 (98.97%), Postives = 579/583 (99.31%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3   VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
           HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYF KAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFFKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KFLFISIMELKSVLA HLPPPLMS LWC++LSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSSLWCNKLSHQPKEVAMSKR 585

BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match: XP_022932574.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita moschata])

HSP 1 Score: 1140 bits (2950), Expect = 0.0
Identity = 576/583 (98.80%), Postives = 578/583 (99.14%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3   VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
           HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYF KAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFFKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KFLFISIMELKSVLA HLPPPLMS LWC++LSHQP EVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSSLWCNKLSHQPNEVAMSKR 585

BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match: XP_022972294.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita maxima])

HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 573/583 (98.28%), Postives = 576/583 (98.80%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELN RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3   VDDRFVELNARTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLIL GLGYRRPSVKLPFKMVWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILEGLGYRRPSVKLPFKMVWNVLLFMKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
           HEKFGFSRYNHSTWARYIQLA CTRTFN VAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLAACTRTFNYVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           SVAQLLLCIVVVLYGYGFIP NIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPANIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYFLKAVTFLY+LKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFLKAVTFLYLLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KFLFISIMELKSVLA HLPPPLMSFLWCD+LSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSFLWCDKLSHQPKEVAMSKR 585

BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match: XP_038897960.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Benincasa hispida])

HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 515/583 (88.34%), Postives = 545/583 (93.48%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELNT+TCVVLGGRGFLGRSLVLRLLKL KWNVRIADS  SLELDPSE DSLLSD
Sbjct: 3   VDDRFVELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAISLELDPSEHDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           AL+AGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIHNGEESLTYPWKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPF+V+QAKSGLAKFIIG GEN+SDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFIVHQAKSGLAKFIIGKGENMSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFW+FISLIL GLGYRRPSVKLPFKMVWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWDFISLILEGLGYRRPSVKLPFKMVWNVLLFMKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
            EKFGF++YNHSTWA YIQLA+CTRTFNCVAAHKQ+GYSPIVS+EEGITLTI+SFSHLRK
Sbjct: 303 DEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLAS TEFT+ESKADKLLGGGRVADILLWREEKKSFTCFLAS LLFYWFF+RGGTFIS
Sbjct: 363 ELSLASFTEFTDESKADKLLGGGRVADILLWREEKKSFTCFLASSLLFYWFFIRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           S AQLLL I+VVLYGYGFIP NIYGFPVQKLS T F  SD+VVRDSIM +ACLWNRG+HN
Sbjct: 423 SAAQLLLSIIVVLYGYGFIPPNIYGFPVQKLSVTTFRQSDSVVRDSIMTLACLWNRGIHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYF KAV FLY+LKL +S++LT+L+GMGLVFAFTAFFVYEQYE EID FA
Sbjct: 483 ARSLARGEDWNYFFKAVAFLYLLKLLVSQALTMLLGMGLVFAFTAFFVYEQYETEIDEFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KF FI IMEL+ +LA HLP PLMSFL CD +SHQ    A++KR
Sbjct: 543 KFFFIGIMELRRLLASHLPTPLMSFLCCDRVSHQ---AAVTKR 582

BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match: A0A6J1EXD3 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439089 PE=4 SV=1)

HSP 1 Score: 1140 bits (2950), Expect = 0.0
Identity = 576/583 (98.80%), Postives = 578/583 (99.14%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3   VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
           HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYF KAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFFKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KFLFISIMELKSVLA HLPPPLMS LWC++LSHQP EVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSSLWCNKLSHQPNEVAMSKR 585

BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match: A0A6J1IB30 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470875 PE=4 SV=1)

HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 573/583 (98.28%), Postives = 576/583 (98.80%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRFVELN RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3   VDDRFVELNARTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLIL GLGYRRPSVKLPFKMVWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILEGLGYRRPSVKLPFKMVWNVLLFMKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
           HEKFGFSRYNHSTWARYIQLA CTRTFN VAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLAACTRTFNYVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           SVAQLLLCIVVVLYGYGFIP NIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPANIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           ARSLARGEDWNYFLKAVTFLY+LKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFLKAVTFLYLLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KFLFISIMELKSVLA HLPPPLMSFLWCD+LSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSFLWCDKLSHQPKEVAMSKR 585

BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match: A0A0A0L5K0 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G174040 PE=4 SV=1)

HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 510/583 (87.48%), Postives = 542/583 (92.97%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRF+ELNT+TCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS  SLELDPSE DSLLSD
Sbjct: 3   VDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           AL+AGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI+A
Sbjct: 63  ALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVISA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPF+V+QAKSG AKFIIGNGEN+SDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFWEFISLIL GLGYRRPSVKLP K+VWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
            EKFGF++YNHSTWA YIQLA+CTRTF+C AAHKQLGYSP+VS+EEGITLTI+SFSHLRK
Sbjct: 303 DEKFGFNKYNHSTWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLAS TEFTEESKADKLLGGGRVAD+LLWR+EKKSFTCFLAS LLFYWFF+RGGTFIS
Sbjct: 363 ELSLASFTEFTEESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFVRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           S AQLLL I++VLYGYGFIP NIYGFPVQKLS T F  SD+VVRDSIM +ACLWNRGVHN
Sbjct: 423 SAAQLLLSIIIVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           AR+LARGEDWNYFLKAV FLY+LKL L+RSLT+L+G+GLVFAFTAFFVYEQYE EID FA
Sbjct: 483 ARALARGEDWNYFLKAVAFLYLLKLLLARSLTMLLGVGLVFAFTAFFVYEQYEAEIDEFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KF FI IMELK +LA HLP PLMSFL CD + H      +SKR
Sbjct: 543 KFFFIGIMELKKLLASHLPTPLMSFLCCDRVPHH---TTVSKR 582

BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match: A0A1S3AXX4 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103483967 PE=4 SV=1)

HSP 1 Score: 1017 bits (2630), Expect = 0.0
Identity = 511/583 (87.65%), Postives = 540/583 (92.62%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRF+ELNT+TCVVLGGRGFLGRSLVLRLLKL KWNVRIADS  SLELDPSE DSLLSD
Sbjct: 3   VDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSANSLELDPSEHDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
           AL+AGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63  ALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122

Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
           CRDCKVRRLIFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDID 182

Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
           GLLTCALRPSNVFGPEDTRLVPFVV+QAKSGLAK IIGNGEN+SDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVHQAKSGLAKLIIGNGENMSDFTFVENVTHAHICAE 242

Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
           EALDFRMVSVAGKAFFITNFKPMKFW+FISLIL GLGYRRPSVKLP K+VWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWQFISLILEGLGYRRPSVKLPCKVVWNVLLFMKWI 302

Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
            EKFGF++YNHSTWA YIQLA+CTRTFNC AAHKQLGYSP+VS+EEGITLTI+SFSHLRK
Sbjct: 303 DEKFGFNKYNHSTWAHYIQLASCTRTFNCSAAHKQLGYSPVVSLEEGITLTIKSFSHLRK 362

Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
           ELSLAS TEFT+ESKADKLLGGGRVAD+LLWR+EKKSFTCFLAS LLFYWFF+RGGTFIS
Sbjct: 363 ELSLASFTEFTDESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFIRGGTFIS 422

Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
           S AQLLL I+VVLYGYGFIP NIYGFPVQKLS T F  SD+VVRDSIM +ACLWNRGVHN
Sbjct: 423 SAAQLLLSIIVVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHN 482

Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
           AR+LARGEDWNYFLKAV  LY+LKL L RSLT+++GMGLVFAFTAFFVYEQYE EID FA
Sbjct: 483 ARALARGEDWNYFLKAVAVLYLLKLLLERSLTMMLGMGLVFAFTAFFVYEQYEAEIDEFA 542

Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
           KF FI IMELK +LA HLP PLMSFL CD + HQ     +SKR
Sbjct: 543 KFFFIGIMELKRLLASHLPTPLMSFLCCDRVPHQ---ATVSKR 582

BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match: A0A5A7VPP8 (Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00240 PE=4 SV=1)

HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 512/634 (80.76%), Postives = 541/634 (85.33%), Query Frame = 0

Query: 12  VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
           VDDRF+ELNT+TCVVLGGRGFLGRSLVLRLLKL KWNVRIADS  SLELDPSE DSLLSD
Sbjct: 3   VDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSANSLELDPSEHDSLLSD 62

Query: 72  ALVAGRASYFHVDVRNKDQIVA-------------------------------------- 131
           ALVAGRASYFHVDVRNKDQIVA                                      
Sbjct: 63  ALVAGRASYFHVDVRNKDQIVAGLLFLSLSVYNAKNSELFPLSVELIALSKDRCMDLDCL 122

Query: 132 -------------ALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRL 191
                        ALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRL
Sbjct: 123 RVVGVDLFYENNDALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRL 182

Query: 192 IFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRP 251
           IFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDIDGLLTCALRP
Sbjct: 183 IFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRP 242

Query: 252 SNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVS 311
           SNVFGPEDTRLVPFVV+QAKSGLAK IIGNGEN+SDFTFVENVTHAHICAEEALDFRMVS
Sbjct: 243 SNVFGPEDTRLVPFVVHQAKSGLAKLIIGNGENMSDFTFVENVTHAHICAEEALDFRMVS 302

Query: 312 VAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRY 371
           VAGKAFFITNFKPMKFW+FISLIL GLGYRRPSVKLP K+VWNVLLF+KWI EKFGF++Y
Sbjct: 303 VAGKAFFITNFKPMKFWQFISLILEGLGYRRPSVKLPCKVVWNVLLFMKWIDEKFGFNKY 362

Query: 372 NHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTE 431
           NHSTWA YIQLA+CTRTFNC AAHKQLGYSP+VS+EEGITLTI+SFSHLRKELSLAS TE
Sbjct: 363 NHSTWAHYIQLASCTRTFNCSAAHKQLGYSPVVSLEEGITLTIKSFSHLRKELSLASFTE 422

Query: 432 FTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCI 491
           FT+ESKADKLLGGGRVAD+LLWR+EKKSFTCFLAS LLFYWFF+RGGTFISS AQLLL I
Sbjct: 423 FTDESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFIRGGTFISSAAQLLLSI 482

Query: 492 VVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGED 551
           +VVLYGYGFIP NIYGFPVQKLS T F  SD+VVRDSIM +ACLWNRGVHNAR+LARGED
Sbjct: 483 IVVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHNARALARGED 542

Query: 552 WNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIME 594
           WNYFLKAV  LY+LKL L+RSLT+++GMGLVFAFTAFFVYEQYE EID FAKF FI IME
Sbjct: 543 WNYFLKAVAVLYLLKLLLARSLTMMLGMGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIME 602

BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match: AT2G43420.1 (3-beta hydroxysteroid dehydrogenase/isomerase family protein )

HSP 1 Score: 568.5 bits (1464), Expect = 6.1e-162
Identity = 299/544 (54.96%), Postives = 378/544 (69.49%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           +TCVVLGGRGF+GRSLV RLL+L  W VR+ADS  +L LD  E DSLL DAL +GRASY 
Sbjct: 13  KTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRASYH 72

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
            VDVR+K QIV    GS VVFYM   D  S+D +  Y  IVQG +NVI+ACR+  VR+LI
Sbjct: 73  CVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVRKLI 132

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           +NS+ADV+FDGS+ I++G+E L  P KF+ ML+D KAQAEALI  AN+ DGLLTCALR S
Sbjct: 133 YNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCALRSS 192

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVSV 261
            VFGP DT  VPF+VN AKSG AKFI+G+GEN+SDFT+ ENV+HAHICA +ALD +M  V
Sbjct: 193 IVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQMEFV 252

Query: 262 AGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRYN 321
           AGK FFITN KP++FW+F+S I+ GLGY RPS+KLP ++V  V   LKW HEK G    N
Sbjct: 253 AGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHEKEGLGS-N 312

Query: 322 HSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEF 381
           + T  +Y  LA+ TRTFNC AA K LGY+P+V++E+GI  T++ FS       L    + 
Sbjct: 313 YDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-----DLEKSDDT 372

Query: 382 TEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIV 441
             +S AD+LLG G+VADILLWR EKK+F  FL   L +YWFF  G TF SS AQLL    
Sbjct: 373 IIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLLFIFA 432

Query: 442 VVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDW 501
           V LYG  F+P+ I+GF V K+    F  S++ VRD    I  +WN+GV + +SL+ G DW
Sbjct: 433 VALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSSGGDW 492

Query: 502 NYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMEL 561
             F K    LY+LKL +SRSL   +   + F+FT FF+YEQYE+E+   A+     +  +
Sbjct: 493 IKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIFVECLTFI 548

Query: 562 KSVL 566
           K ++
Sbjct: 553 KRMV 548

BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match: AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 232/551 (42.11%), Postives = 331/551 (60.07%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           R CVV GGRGF  R LV  L++   + VRIAD   ++ LDP E + +L + L +GR  Y 
Sbjct: 10  RWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYI 69

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+R+K Q+V A  G+ VVF+M   DS  N+  L Y   VQG +NVI+AC D  V+RLI
Sbjct: 70  SADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLI 129

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           + SS  V+FDG   I NG E +AYP K  D  S  KA+ E LI+ AN  +GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPS 189

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
           ++FGP D  LVP +V  A++G +KFIIG+G NL DFT+VENV HAH+CAE AL       
Sbjct: 190 SIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVS 249

Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
              AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P  ++  +   ++  ++  G +
Sbjct: 250 TKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPY 309

Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
                      ++L +C+RTF+   A  +LGY+P+V ++EGI  TI+SFSH    L+  S
Sbjct: 310 GMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH----LTAGS 369

Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLL 441
            ++    SKA ++LGGG+VAD LLW++ K++         ++Y F   G T ++++++ L
Sbjct: 370 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 429

Query: 442 LCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLAR 501
           L   V L+ +G +P  I+G+ V+K+ A+ F  S     D  + +   WN  V   RSL +
Sbjct: 430 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 489

Query: 502 GEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFIS 561
           G DW++F K V  L  L L  + SL  +  +GL  AF AF VYE+ E EID         
Sbjct: 490 GNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSI------- 549

Query: 562 IMELKSVLARH 569
           ++  KS   +H
Sbjct: 550 VVSFKSFACKH 549

BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match: AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )

HSP 1 Score: 351.7 bits (901), Expect = 1.2e-96
Identity = 194/465 (41.72%), Postives = 279/465 (60.00%), Query Frame = 0

Query: 108 DSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPR 167
           DS  N+  L Y   VQG +NVI+AC D  V+RLI+ SS  V+FDG   I NG E +AYP 
Sbjct: 5   DSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAYPI 64

Query: 168 KFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFI 227
           K  D  S  KA+ E LI+ AN  +GLLTC +RPS++FGP D  LVP +V  A++G +KFI
Sbjct: 65  KHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFI 124

Query: 228 IGNGENLSDFTFVENVTHAHICAEEALDFR---MVSVAGKAFFITNFKPMKFWEFISLIL 287
           IG+G NL DFT+VENV HAH+CAE AL          AG+A+FITN +P+KFWEF+S +L
Sbjct: 125 IGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFMSQLL 184

Query: 288 GGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-FSRYNHSTWARYIQLATCTRTFNCVAA 347
            GLGY RPS+K+P  ++  +   ++  ++  G +           ++L +C+RTF+   A
Sbjct: 185 DGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFDSTKA 244

Query: 348 HKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEFTEESKADKLLGGGRVADILLWR 407
             +LGY+P+V ++EGI  TI+SFSH    L+  S ++    SKA ++LGGG+VAD LLW+
Sbjct: 245 KDRLGYAPVVPLQEGIRRTIDSFSH----LTAGSQSKREGPSKASRILGGGKVADTLLWK 304

Query: 408 EEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLS 467
           + K++         ++Y F   G T ++++++ LL   V L+ +G +P  I+G+ V+K+ 
Sbjct: 305 DLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIP 364

Query: 468 ATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDWNYFLKAVTFLYVLKLFLSRSLT 527
           A+ F  S     D  + +   WN  V   RSL +G DW++F K V  L  L L  + SL 
Sbjct: 365 ASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLH 424

Query: 528 LLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMELKSVLARH 569
            +  +GL  AF AF VYE+ E EID         ++  KS   +H
Sbjct: 425 SIFVIGLPIAFLAFLVYEKKEQEIDSI-------VVSFKSFACKH 458

BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match: AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 317.8 bits (813), Expect = 1.9e-86
Identity = 178/448 (39.73%), Postives = 263/448 (58.71%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           R CVV GGRGF  R LV  L++   ++VRIAD   ++ L+P E+  +L +A+ +GR  Y 
Sbjct: 10  RWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYV 69

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+RNK Q+V    G+ VVF+M   DS  N+  L Y   VQG  NVI+AC +  V+RLI
Sbjct: 70  SADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLI 129

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           + SS  V+FDG     N +E L YP K  D  S  KA+ EALIL AN   GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPS 189

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
           ++FGP D  +VP +V  A++G +KFIIG+G N  DFT+VENV HAH+CAE AL       
Sbjct: 190 SIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVC 249

Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
              AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P  ++  +   ++  ++  G +
Sbjct: 250 AKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPY 309

Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
                      ++L +C RTF+   A  +LGYSP+V ++EGI  TI+SFSHL+ +     
Sbjct: 310 GMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ----- 369

Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGT---FISSVA 441
                 + K +       V + + W+  K++    +    L++ F    G+    I++V+
Sbjct: 370 -----NQPKTE-------VTETIQWK--KQTLIAIVILITLYHNFVATTGSSSVIITAVS 429

Query: 442 QLLLCIVVVLYGYGFIPTNIYGFPVQKL 463
           ++LL   + ++  G +P  +  F  +K+
Sbjct: 430 KVLLVSSIFMFINGILPEKMKVFGSKKI 438

BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match: AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )

HSP 1 Score: 317.4 bits (812), Expect = 2.5e-86
Identity = 163/355 (45.92%), Postives = 225/355 (63.38%), Query Frame = 0

Query: 22  RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
           R CVV GGRGF  R LV  L++   ++VRIAD   ++ L+P E+  +L +A+ +GR  Y 
Sbjct: 10  RWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYV 69

Query: 82  HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
             D+RNK Q+V    G+ VVF+M   DS  N+  L Y   VQG  NVI+AC +  V+RLI
Sbjct: 70  SADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLI 129

Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
           + SS  V+FDG     N +E L YP K  D  S  KA+ EALIL AN   GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPS 189

Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
           ++FGP D  +VP +V  A++G +KFIIG+G N  DFT+VENV HAH+CAE AL       
Sbjct: 190 SIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVC 249

Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
              AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P  ++  +   ++  ++  G +
Sbjct: 250 AKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPY 309

Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKE 373
                      ++L +C RTF+   A  +LGYSP+V ++EGI  TI+SFSHL+ +
Sbjct: 310 GMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ 364

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A9X4U28.6e-16154.963beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thalia... [more]
Q67ZE12.2e-11642.113beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thalia... [more]
Q9FX012.6e-8539.733beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thalia... [more]
Q5PPL31.4e-5737.68Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus... [more]
Q9R1J04.0e-5738.24Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=1... [more]
Match NameE-valueIdentityDescription
XP_023540745.10.0100.003beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita pepo subsp. pepo][more]
KAG6597621.10.098.973beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3, partial [Cucurbita a... [more]
XP_022932574.10.098.803beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita moschata][more]
XP_022972294.10.098.283beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita maxima][more]
XP_038897960.10.088.343beta-hydroxysteroid-dehydrogenase/decarboxylase [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1EXD30.098.80Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439089 PE=4 SV=1[more]
A0A6J1IB300.098.28Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470875 PE=4 SV=1[more]
A0A0A0L5K00.087.48Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G174040 PE=4 SV=1[more]
A0A1S3AXX40.087.65Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103483967 PE=4 SV=1[more]
A0A5A7VPP80.080.76Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5... [more]
Match NameE-valueIdentityDescription
AT2G43420.16.1e-16254.963-beta hydroxysteroid dehydrogenase/isomerase family protein [more]
AT2G26260.11.6e-11742.113beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [more]
AT2G26260.21.2e-9641.723beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 [more]
AT1G47290.21.9e-8639.733beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [more]
AT1G47290.12.5e-8645.923beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003388ReticulonPFAMPF02453Reticuloncoord: 396..552
e-value: 6.5E-33
score: 113.9
IPR003388ReticulonPROSITEPS50845RETICULONcoord: 396..594
score: 13.787559
IPR0022253-beta hydroxysteroid dehydrogenase/isomerasePFAMPF010733Beta_HSDcoord: 25..297
e-value: 2.8E-74
score: 249.7
NoneNo IPR availableGENE3D3.40.50.720coord: 15..371
e-value: 8.9E-51
score: 175.0
NoneNo IPR availablePANTHERPTHR10366:SF6393BETA-HYDROXYSTEROID-DEHYDROGENASE/DECARBOXYLASE ISOFORM 3coord: 20..502
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 20..502
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 19..369

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG08g05780.1Cp4.1LG08g05780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006694 steroid biosynthetic process
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor