Homology
BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match:
A9X4U2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D3 PE=2 SV=2)
HSP 1 Score: 568.5 bits (1464), Expect = 8.6e-161
Identity = 299/544 (54.96%), Postives = 378/544 (69.49%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
+TCVVLGGRGF+GRSLV RLL+L W VR+ADS +L LD E DSLL DAL +GRASY
Sbjct: 13 KTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRASYH 72
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
VDVR+K QIV GS VVFYM D S+D + Y IVQG +NVI+ACR+ VR+LI
Sbjct: 73 CVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVRKLI 132
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+NS+ADV+FDGS+ I++G+E L P KF+ ML+D KAQAEALI AN+ DGLLTCALR S
Sbjct: 133 YNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCALRSS 192
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVSV 261
VFGP DT VPF+VN AKSG AKFI+G+GEN+SDFT+ ENV+HAHICA +ALD +M V
Sbjct: 193 IVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQMEFV 252
Query: 262 AGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRYN 321
AGK FFITN KP++FW+F+S I+ GLGY RPS+KLP ++V V LKW HEK G N
Sbjct: 253 AGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHEKEGLGS-N 312
Query: 322 HSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEF 381
+ T +Y LA+ TRTFNC AA K LGY+P+V++E+GI T++ FS L +
Sbjct: 313 YDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-----DLEKSDDT 372
Query: 382 TEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIV 441
+S AD+LLG G+VADILLWR EKK+F FL L +YWFF G TF SS AQLL
Sbjct: 373 IIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLLFIFA 432
Query: 442 VVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDW 501
V LYG F+P+ I+GF V K+ F S++ VRD I +WN+GV + +SL+ G DW
Sbjct: 433 VALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSSGGDW 492
Query: 502 NYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMEL 561
F K LY+LKL +SRSL + + F+FT FF+YEQYE+E+ A+ + +
Sbjct: 493 IKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIFVECLTFI 548
Query: 562 KSVL 566
K ++
Sbjct: 553 KRMV 548
BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match:
Q67ZE1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D2 PE=1 SV=2)
HSP 1 Score: 421.0 bits (1081), Expect = 2.2e-116
Identity = 232/551 (42.11%), Postives = 331/551 (60.07%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
R CVV GGRGF R LV L++ + VRIAD ++ LDP E + +L + L +GR Y
Sbjct: 10 RWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYI 69
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+R+K Q+V A G+ VVF+M DS N+ L Y VQG +NVI+AC D V+RLI
Sbjct: 70 SADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLI 129
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+ SS V+FDG I NG E +AYP K D S KA+ E LI+ AN +GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPS 189
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
++FGP D LVP +V A++G +KFIIG+G NL DFT+VENV HAH+CAE AL
Sbjct: 190 SIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVS 249
Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P ++ + ++ ++ G +
Sbjct: 250 TKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPY 309
Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
++L +C+RTF+ A +LGY+P+V ++EGI TI+SFSH L+ S
Sbjct: 310 GMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH----LTAGS 369
Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLL 441
++ SKA ++LGGG+VAD LLW++ K++ ++Y F G T ++++++ L
Sbjct: 370 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 429
Query: 442 LCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLAR 501
L V L+ +G +P I+G+ V+K+ A+ F S D + + WN V RSL +
Sbjct: 430 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 489
Query: 502 GEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFIS 561
G DW++F K V L L L + SL + +GL AF AF VYE+ E EID
Sbjct: 490 GNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSI------- 549
Query: 562 IMELKSVLARH 569
++ KS +H
Sbjct: 550 VVSFKSFACKH 549
BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match:
Q9FX01 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thaliana OX=3702 GN=3BETAHSD/D1 PE=1 SV=1)
HSP 1 Score: 317.8 bits (813), Expect = 2.6e-85
Identity = 178/448 (39.73%), Postives = 263/448 (58.71%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
R CVV GGRGF R LV L++ ++VRIAD ++ L+P E+ +L +A+ +GR Y
Sbjct: 10 RWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYV 69
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+RNK Q+V G+ VVF+M DS N+ L Y VQG NVI+AC + V+RLI
Sbjct: 70 SADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLI 129
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+ SS V+FDG N +E L YP K D S KA+ EALIL AN GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPS 189
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
++FGP D +VP +V A++G +KFIIG+G N DFT+VENV HAH+CAE AL
Sbjct: 190 SIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVC 249
Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P ++ + ++ ++ G +
Sbjct: 250 AKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPY 309
Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
++L +C RTF+ A +LGYSP+V ++EGI TI+SFSHL+ +
Sbjct: 310 GMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ----- 369
Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGT---FISSVA 441
+ K + V + + W+ K++ + L++ F G+ I++V+
Sbjct: 370 -----NQPKTE-------VTETIQWK--KQTLIAIVILITLYHNFVATTGSSSVIITAVS 429
Query: 442 QLLLCIVVVLYGYGFIPTNIYGFPVQKL 463
++LL + ++ G +P + F +K+
Sbjct: 430 KVLLVSSIFMFINGILPEKMKVFGSKKI 438
BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match:
Q5PPL3 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus OX=10116 GN=Nsdhl PE=2 SV=1)
HSP 1 Score: 225.7 bits (574), Expect = 1.4e-57
Identity = 133/353 (37.68%), Postives = 195/353 (55.24%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
+ C V+GG GFLG+ +V +LL + V + D + + +P R +F
Sbjct: 27 KKCTVIGGSGFLGQHMVEQLLSRG-YAVNVFDVRQGFD-NP--------------RVQFF 86
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+ N+ + AL G S VF+ S+SN+ L Y G K VI C++ V++LI
Sbjct: 87 IGDLCNQQDLYPALKGVSTVFHCASPPSNSNNKELFYRVNSTGTKTVIETCKEAGVQKLI 146
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFAND-IDGLLTCALRP 201
SSA V+F+G DI+NG E L Y K D ++ K E +L AND LT A+RP
Sbjct: 147 LTSSASVVFEGV-DIKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAAIRP 206
Query: 202 SNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVS 261
+FGP D +LVP +++ A+ G KF+IGNG+NL DFTFVENV H HI A E L R
Sbjct: 207 HGIFGPRDPQLVPVLIDAARKGKMKFMIGNGKNLVDFTFVENVVHGHILAAEHLS-RDAG 266
Query: 262 VAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRY 321
+ GKAF ITN +P+ FW F+S IL GL Y P +P+++ + + L +
Sbjct: 267 LGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYRVAYYLAFLLSLLVMVLSPLIQ 326
Query: 322 NHSTWARY-IQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKE 373
+T+ + + LA ++C A K +GY P+V+M++ + T++SF HLRK+
Sbjct: 327 IQTTFTPFRVALAGTFHYYSCEKAKKLIGYRPLVTMDDAVERTVQSFHHLRKD 361
BLAST of Cp4.1LG08g05780 vs. ExPASy Swiss-Prot
Match:
Q9R1J0 (Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=10090 GN=Nsdhl PE=1 SV=1)
HSP 1 Score: 224.2 bits (570), Expect = 4.0e-57
Identity = 135/353 (38.24%), Postives = 192/353 (54.39%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
+ C V+GG GFLG+ +V +LL+ + V + D + + +P R +F
Sbjct: 27 KKCTVIGGSGFLGQHMVEQLLERG-YTVNVFDIHQGFD-NP--------------RVQFF 86
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+ N+ + AL G S VF+ +SN+ L Y G K VI CR+ V++LI
Sbjct: 87 IGDLCNQQDLYPALKGVSTVFHCASPPPYSNNKELFYRVNFIGTKTVIETCREAGVQKLI 146
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFAND-IDGLLTCALRP 201
SSA V+F+G DI+NG E L Y K D ++ K E +L AND LT A+RP
Sbjct: 147 LTSSASVVFEGV-DIKNGTEDLPYAMKPIDYYTETKILQERAVLDANDPKKNFLTAAIRP 206
Query: 202 SNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVS 261
+FGP D +LVP +++ A+ G KF+IGNGENL DFTFVENV H HI A E L + +
Sbjct: 207 HGIFGPRDPQLVPILIDAARKGKMKFMIGNGENLVDFTFVENVVHGHILAAEHLS-QDAA 266
Query: 262 VAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRY 321
+ GKAF ITN +P+ FW F+S IL GL Y P +P+ M + + L +
Sbjct: 267 LGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWMAYYLAFLLSLLVMVVSPLIQ 326
Query: 322 NHSTWARY-IQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKE 373
T+ + LA ++C A K GY P+V+M+E + T++SF HLRK+
Sbjct: 327 IQPTFTPIRVALAGTFHYYSCEKAKKLFGYRPLVTMDEAVERTVQSFHHLRKD 361
BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match:
XP_023540745.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1154 bits (2986), Expect = 0.0
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD
Sbjct: 3 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 585
BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match:
KAG6597621.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029062.1 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1142 bits (2955), Expect = 0.0
Identity = 577/583 (98.97%), Postives = 579/583 (99.31%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYF KAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFFKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KFLFISIMELKSVLA HLPPPLMS LWC++LSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSSLWCNKLSHQPKEVAMSKR 585
BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match:
XP_022932574.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita moschata])
HSP 1 Score: 1140 bits (2950), Expect = 0.0
Identity = 576/583 (98.80%), Postives = 578/583 (99.14%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYF KAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFFKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KFLFISIMELKSVLA HLPPPLMS LWC++LSHQP EVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSSLWCNKLSHQPNEVAMSKR 585
BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match:
XP_022972294.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita maxima])
HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 573/583 (98.28%), Postives = 576/583 (98.80%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELN RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3 VDDRFVELNARTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLIL GLGYRRPSVKLPFKMVWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILEGLGYRRPSVKLPFKMVWNVLLFMKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
HEKFGFSRYNHSTWARYIQLA CTRTFN VAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLAACTRTFNYVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
SVAQLLLCIVVVLYGYGFIP NIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPANIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYFLKAVTFLY+LKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFLKAVTFLYLLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KFLFISIMELKSVLA HLPPPLMSFLWCD+LSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSFLWCDKLSHQPKEVAMSKR 585
BLAST of Cp4.1LG08g05780 vs. NCBI nr
Match:
XP_038897960.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase [Benincasa hispida])
HSP 1 Score: 1027 bits (2656), Expect = 0.0
Identity = 515/583 (88.34%), Postives = 545/583 (93.48%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELNT+TCVVLGGRGFLGRSLVLRLLKL KWNVRIADS SLELDPSE DSLLSD
Sbjct: 3 VDDRFVELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSAISLELDPSEHDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
AL+AGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIHNGEESLTYPWKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPF+V+QAKSGLAKFIIG GEN+SDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFIVHQAKSGLAKFIIGKGENMSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFW+FISLIL GLGYRRPSVKLPFKMVWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWDFISLILEGLGYRRPSVKLPFKMVWNVLLFMKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
EKFGF++YNHSTWA YIQLA+CTRTFNCVAAHKQ+GYSPIVS+EEGITLTI+SFSHLRK
Sbjct: 303 DEKFGFNKYNHSTWAHYIQLASCTRTFNCVAAHKQIGYSPIVSLEEGITLTIKSFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLAS TEFT+ESKADKLLGGGRVADILLWREEKKSFTCFLAS LLFYWFF+RGGTFIS
Sbjct: 363 ELSLASFTEFTDESKADKLLGGGRVADILLWREEKKSFTCFLASSLLFYWFFIRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
S AQLLL I+VVLYGYGFIP NIYGFPVQKLS T F SD+VVRDSIM +ACLWNRG+HN
Sbjct: 423 SAAQLLLSIIVVLYGYGFIPPNIYGFPVQKLSVTTFRQSDSVVRDSIMTLACLWNRGIHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYF KAV FLY+LKL +S++LT+L+GMGLVFAFTAFFVYEQYE EID FA
Sbjct: 483 ARSLARGEDWNYFFKAVAFLYLLKLLVSQALTMLLGMGLVFAFTAFFVYEQYETEIDEFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KF FI IMEL+ +LA HLP PLMSFL CD +SHQ A++KR
Sbjct: 543 KFFFIGIMELRRLLASHLPTPLMSFLCCDRVSHQ---AAVTKR 582
BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match:
A0A6J1EXD3 (Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439089 PE=4 SV=1)
HSP 1 Score: 1140 bits (2950), Expect = 0.0
Identity = 576/583 (98.80%), Postives = 578/583 (99.14%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYF KAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFFKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KFLFISIMELKSVLA HLPPPLMS LWC++LSHQP EVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSSLWCNKLSHQPNEVAMSKR 585
BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match:
A0A6J1IB30 (Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470875 PE=4 SV=1)
HSP 1 Score: 1134 bits (2933), Expect = 0.0
Identity = 573/583 (98.28%), Postives = 576/583 (98.80%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRFVELN RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS ESLELDPSEQDSLLSD
Sbjct: 3 VDDRFVELNARTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSAESLELDPSEQDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLIL GLGYRRPSVKLPFKMVWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILEGLGYRRPSVKLPFKMVWNVLLFMKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
HEKFGFSRYNHSTWARYIQLA CTRTFN VAAHKQLGYSPIVSMEEGITLTIESFSHLRK
Sbjct: 303 HEKFGFSRYNHSTWARYIQLAACTRTFNYVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS
Sbjct: 363 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
SVAQLLLCIVVVLYGYGFIP NIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN
Sbjct: 423 SVAQLLLCIVVVLYGYGFIPANIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
ARSLARGEDWNYFLKAVTFLY+LKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA
Sbjct: 483 ARSLARGEDWNYFLKAVTFLYLLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KFLFISIMELKSVLA HLPPPLMSFLWCD+LSHQPKEVAMSKR
Sbjct: 543 KFLFISIMELKSVLASHLPPPLMSFLWCDKLSHQPKEVAMSKR 585
BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match:
A0A0A0L5K0 (Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G174040 PE=4 SV=1)
HSP 1 Score: 1018 bits (2633), Expect = 0.0
Identity = 510/583 (87.48%), Postives = 542/583 (92.97%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRF+ELNT+TCVVLGGRGFLGRSLVLRLLKLAKWNVRIADS SLELDPSE DSLLSD
Sbjct: 3 VDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSANSLELDPSEHDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
AL+AGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVI+A
Sbjct: 63 ALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVISA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPF+V+QAKSG AKFIIGNGEN+SDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFIVHQAKSGFAKFIIGNGENMSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFWEFISLIL GLGYRRPSVKLP K+VWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILRGLGYRRPSVKLPSKVVWNVLLFMKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
EKFGF++YNHSTWA YIQLA+CTRTF+C AAHKQLGYSP+VS+EEGITLTI+SFSHLRK
Sbjct: 303 DEKFGFNKYNHSTWAHYIQLASCTRTFSCAAAHKQLGYSPVVSLEEGITLTIKSFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLAS TEFTEESKADKLLGGGRVAD+LLWR+EKKSFTCFLAS LLFYWFF+RGGTFIS
Sbjct: 363 ELSLASFTEFTEESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFVRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
S AQLLL I++VLYGYGFIP NIYGFPVQKLS T F SD+VVRDSIM +ACLWNRGVHN
Sbjct: 423 SAAQLLLSIIIVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
AR+LARGEDWNYFLKAV FLY+LKL L+RSLT+L+G+GLVFAFTAFFVYEQYE EID FA
Sbjct: 483 ARALARGEDWNYFLKAVAFLYLLKLLLARSLTMLLGVGLVFAFTAFFVYEQYEAEIDEFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KF FI IMELK +LA HLP PLMSFL CD + H +SKR
Sbjct: 543 KFFFIGIMELKKLLASHLPTPLMSFLCCDRVPHH---TTVSKR 582
BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match:
A0A1S3AXX4 (Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103483967 PE=4 SV=1)
HSP 1 Score: 1017 bits (2630), Expect = 0.0
Identity = 511/583 (87.65%), Postives = 540/583 (92.62%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRF+ELNT+TCVVLGGRGFLGRSLVLRLLKL KWNVRIADS SLELDPSE DSLLSD
Sbjct: 3 VDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSANSLELDPSEHDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 131
AL+AGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA
Sbjct: 63 ALIAGRASYFHVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINA 122
Query: 132 CRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDID 191
CRDCKVRRLIFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDID
Sbjct: 123 CRDCKVRRLIFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDID 182
Query: 192 GLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAE 251
GLLTCALRPSNVFGPEDTRLVPFVV+QAKSGLAK IIGNGEN+SDFTFVENVTHAHICAE
Sbjct: 183 GLLTCALRPSNVFGPEDTRLVPFVVHQAKSGLAKLIIGNGENMSDFTFVENVTHAHICAE 242
Query: 252 EALDFRMVSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWI 311
EALDFRMVSVAGKAFFITNFKPMKFW+FISLIL GLGYRRPSVKLP K+VWNVLLF+KWI
Sbjct: 243 EALDFRMVSVAGKAFFITNFKPMKFWQFISLILEGLGYRRPSVKLPCKVVWNVLLFMKWI 302
Query: 312 HEKFGFSRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRK 371
EKFGF++YNHSTWA YIQLA+CTRTFNC AAHKQLGYSP+VS+EEGITLTI+SFSHLRK
Sbjct: 303 DEKFGFNKYNHSTWAHYIQLASCTRTFNCSAAHKQLGYSPVVSLEEGITLTIKSFSHLRK 362
Query: 372 ELSLASLTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFIS 431
ELSLAS TEFT+ESKADKLLGGGRVAD+LLWR+EKKSFTCFLAS LLFYWFF+RGGTFIS
Sbjct: 363 ELSLASFTEFTDESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFIRGGTFIS 422
Query: 432 SVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHN 491
S AQLLL I+VVLYGYGFIP NIYGFPVQKLS T F SD+VVRDSIM +ACLWNRGVHN
Sbjct: 423 SAAQLLLSIIVVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHN 482
Query: 492 ARSLARGEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFA 551
AR+LARGEDWNYFLKAV LY+LKL L RSLT+++GMGLVFAFTAFFVYEQYE EID FA
Sbjct: 483 ARALARGEDWNYFLKAVAVLYLLKLLLERSLTMMLGMGLVFAFTAFFVYEQYEAEIDEFA 542
Query: 552 KFLFISIMELKSVLARHLPPPLMSFLWCDELSHQPKEVAMSKR 594
KF FI IMELK +LA HLP PLMSFL CD + HQ +SKR
Sbjct: 543 KFFFIGIMELKRLLASHLPTPLMSFLCCDRVPHQ---ATVSKR 582
BLAST of Cp4.1LG08g05780 vs. ExPASy TrEMBL
Match:
A0A5A7VPP8 (Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00240 PE=4 SV=1)
HSP 1 Score: 994 bits (2570), Expect = 0.0
Identity = 512/634 (80.76%), Postives = 541/634 (85.33%), Query Frame = 0
Query: 12 VDDRFVELNTRTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSD 71
VDDRF+ELNT+TCVVLGGRGFLGRSLVLRLLKL KWNVRIADS SLELDPSE DSLLSD
Sbjct: 3 VDDRFLELNTKTCVVLGGRGFLGRSLVLRLLKLGKWNVRIADSANSLELDPSEHDSLLSD 62
Query: 72 ALVAGRASYFHVDVRNKDQIVA-------------------------------------- 131
ALVAGRASYFHVDVRNKDQIVA
Sbjct: 63 ALVAGRASYFHVDVRNKDQIVAGLLFLSLSVYNAKNSELFPLSVELIALSKDRCMDLDCL 122
Query: 132 -------------ALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRL 191
ALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRL
Sbjct: 123 RVVGVDLFYENNDALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRL 182
Query: 192 IFNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRP 251
IFNSSADVIFDGSRDI NGEE L YP KFEDMLSDLKAQAEALILFANDIDGLLTCALRP
Sbjct: 183 IFNSSADVIFDGSRDILNGEESLTYPWKFEDMLSDLKAQAEALILFANDIDGLLTCALRP 242
Query: 252 SNVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVS 311
SNVFGPEDTRLVPFVV+QAKSGLAK IIGNGEN+SDFTFVENVTHAHICAEEALDFRMVS
Sbjct: 243 SNVFGPEDTRLVPFVVHQAKSGLAKLIIGNGENMSDFTFVENVTHAHICAEEALDFRMVS 302
Query: 312 VAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRY 371
VAGKAFFITNFKPMKFW+FISLIL GLGYRRPSVKLP K+VWNVLLF+KWI EKFGF++Y
Sbjct: 303 VAGKAFFITNFKPMKFWQFISLILEGLGYRRPSVKLPCKVVWNVLLFMKWIDEKFGFNKY 362
Query: 372 NHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTE 431
NHSTWA YIQLA+CTRTFNC AAHKQLGYSP+VS+EEGITLTI+SFSHLRKELSLAS TE
Sbjct: 363 NHSTWAHYIQLASCTRTFNCSAAHKQLGYSPVVSLEEGITLTIKSFSHLRKELSLASFTE 422
Query: 432 FTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCI 491
FT+ESKADKLLGGGRVAD+LLWR+EKKSFTCFLAS LLFYWFF+RGGTFISS AQLLL I
Sbjct: 423 FTDESKADKLLGGGRVADVLLWRDEKKSFTCFLASSLLFYWFFIRGGTFISSAAQLLLSI 482
Query: 492 VVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGED 551
+VVLYGYGFIP NIYGFPVQKLS T F SD+VVRDSIM +ACLWNRGVHNAR+LARGED
Sbjct: 483 IVVLYGYGFIPPNIYGFPVQKLSVTAFRQSDSVVRDSIMTLACLWNRGVHNARALARGED 542
Query: 552 WNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIME 594
WNYFLKAV LY+LKL L+RSLT+++GMGLVFAFTAFFVYEQYE EID FAKF FI IME
Sbjct: 543 WNYFLKAVAVLYLLKLLLARSLTMMLGMGLVFAFTAFFVYEQYEAEIDEFAKFFFIGIME 602
BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match:
AT2G43420.1 (3-beta hydroxysteroid dehydrogenase/isomerase family protein )
HSP 1 Score: 568.5 bits (1464), Expect = 6.1e-162
Identity = 299/544 (54.96%), Postives = 378/544 (69.49%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
+TCVVLGGRGF+GRSLV RLL+L W VR+ADS +L LD E DSLL DAL +GRASY
Sbjct: 13 KTCVVLGGRGFIGRSLVSRLLRLGNWTVRVADSGHTLHLD--ESDSLLEDALSSGRASYH 72
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
VDVR+K QIV GS VVFYM D S+D + Y IVQG +NVI+ACR+ VR+LI
Sbjct: 73 CVDVRDKPQIVKVTEGSYVVFYMGATDLRSHDYFDCYKVIVQGTRNVISACRESGVRKLI 132
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+NS+ADV+FDGS+ I++G+E L P KF+ ML+D KAQAEALI AN+ DGLLTCALR S
Sbjct: 133 YNSTADVVFDGSQPIRDGDESLRRPLKFQSMLTDFKAQAEALIKLANNRDGLLTCALRSS 192
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFRMVSV 261
VFGP DT VPF+VN AKSG AKFI+G+GEN+SDFT+ ENV+HAHICA +ALD +M V
Sbjct: 193 IVFGPGDTEFVPFLVNLAKSGYAKFILGSGENISDFTYSENVSHAHICAVKALDSQMEFV 252
Query: 262 AGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFGFSRYN 321
AGK FFITN KP++FW+F+S I+ GLGY RPS+KLP ++V V LKW HEK G N
Sbjct: 253 AGKEFFITNLKPVRFWDFVSHIVEGLGYPRPSIKLPVRLVLYVFSLLKWTHEKEGLGS-N 312
Query: 322 HSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEF 381
+ T +Y LA+ TRTFNC AA K LGY+P+V++E+GI T++ FS L +
Sbjct: 313 YDTAHQYALLASSTRTFNCNAAKKHLGYTPVVTLEDGIASTLQWFSR-----DLEKSDDT 372
Query: 382 TEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIV 441
+S AD+LLG G+VADILLWR EKK+F FL L +YWFF G TF SS AQLL
Sbjct: 373 IIQSTADQLLGCGKVADILLWRNEKKTFVSFLVLNLFYYWFFFSGNTFTSSAAQLLFIFA 432
Query: 442 VVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDW 501
V LYG F+P+ I+GF V K+ F S++ VRD I +WN+GV + +SL+ G DW
Sbjct: 433 VALYGVSFVPSKIFGFQVNKIPPWRFEISESAVRDLSSDIVVVWNQGVRSFKSLSSGGDW 492
Query: 502 NYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMEL 561
F K LY+LKL +SRSL + + F+FT FF+YEQYE+E+ A+ + +
Sbjct: 493 IKFFKIAGSLYLLKLIVSRSLAAFLFTVMSFSFTGFFIYEQYELELYHLARIFVECLTFI 548
Query: 562 KSVL 566
K ++
Sbjct: 553 KRMV 548
BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match:
AT2G26260.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )
HSP 1 Score: 421.0 bits (1081), Expect = 1.6e-117
Identity = 232/551 (42.11%), Postives = 331/551 (60.07%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
R CVV GGRGF R LV L++ + VRIAD ++ LDP E + +L + L +GR Y
Sbjct: 10 RWCVVTGGRGFAARHLVEMLVRYEMFCVRIADLAPAIMLDPQEGNGVLDEGLRSGRVQYI 69
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+R+K Q+V A G+ VVF+M DS N+ L Y VQG +NVI+AC D V+RLI
Sbjct: 70 SADLRDKSQVVKAFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLI 129
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+ SS V+FDG I NG E +AYP K D S KA+ E LI+ AN +GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGILNGTESMAYPIKHNDSYSATKAEGEELIMKANGRNGLLTCCIRPS 189
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
++FGP D LVP +V A++G +KFIIG+G NL DFT+VENV HAH+CAE AL
Sbjct: 190 SIFGPGDRLLVPSLVAAARAGKSKFIIGDGNNLYDFTYVENVAHAHVCAERALASGGDVS 249
Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P ++ + ++ ++ G +
Sbjct: 250 TKAAGQAYFITNMEPIKFWEFMSQLLDGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPY 309
Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
++L +C+RTF+ A +LGY+P+V ++EGI TI+SFSH L+ S
Sbjct: 310 GMTVPQLTPSRVRLLSCSRTFDSTKAKDRLGYAPVVPLQEGIRRTIDSFSH----LTAGS 369
Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLL 441
++ SKA ++LGGG+VAD LLW++ K++ ++Y F G T ++++++ L
Sbjct: 370 QSKREGPSKASRILGGGKVADTLLWKDLKQTLIAIFILISIYYNFVATGSTVVTALSKAL 429
Query: 442 LCIVVVLYGYGFIPTNIYGFPVQKLSATMFGPSDTVVRDSIMGIACLWNRGVHNARSLAR 501
L V L+ +G +P I+G+ V+K+ A+ F S D + + WN V RSL +
Sbjct: 430 LVASVFLFLHGILPEKIFGYTVEKIPASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQ 489
Query: 502 GEDWNYFLKAVTFLYVLKLFLSRSLTLLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFIS 561
G DW++F K V L L L + SL + +GL AF AF VYE+ E EID
Sbjct: 490 GNDWSFFFKVVFVLLALSLAGAISLHSIFVIGLPIAFLAFLVYEKKEQEIDSI------- 549
Query: 562 IMELKSVLARH 569
++ KS +H
Sbjct: 550 VVSFKSFACKH 549
BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match:
AT2G26260.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 )
HSP 1 Score: 351.7 bits (901), Expect = 1.2e-96
Identity = 194/465 (41.72%), Postives = 279/465 (60.00%), Query Frame = 0
Query: 108 DSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLIFNSSADVIFDGSRDIQNGEEPLAYPR 167
DS N+ L Y VQG +NVI+AC D V+RLI+ SS V+FDG I NG E +AYP
Sbjct: 5 DSSINNHQLQYSVNVQGTQNVIDACVDVGVKRLIYTSSPSVVFDGVHGILNGTESMAYPI 64
Query: 168 KFEDMLSDLKAQAEALILFANDIDGLLTCALRPSNVFGPEDTRLVPFVVNQAKSGLAKFI 227
K D S KA+ E LI+ AN +GLLTC +RPS++FGP D LVP +V A++G +KFI
Sbjct: 65 KHNDSYSATKAEGEELIMKANGRNGLLTCCIRPSSIFGPGDRLLVPSLVAAARAGKSKFI 124
Query: 228 IGNGENLSDFTFVENVTHAHICAEEALDFR---MVSVAGKAFFITNFKPMKFWEFISLIL 287
IG+G NL DFT+VENV HAH+CAE AL AG+A+FITN +P+KFWEF+S +L
Sbjct: 125 IGDGNNLYDFTYVENVAHAHVCAERALASGGDVSTKAAGQAYFITNMEPIKFWEFMSQLL 184
Query: 288 GGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-FSRYNHSTWARYIQLATCTRTFNCVAA 347
GLGY RPS+K+P ++ + ++ ++ G + ++L +C+RTF+ A
Sbjct: 185 DGLGYERPSIKIPAFIMMPIAHLVELTYKVLGPYGMTVPQLTPSRVRLLSCSRTFDSTKA 244
Query: 348 HKQLGYSPIVSMEEGITLTIESFSHLRKELSLASLTEFTEESKADKLLGGGRVADILLWR 407
+LGY+P+V ++EGI TI+SFSH L+ S ++ SKA ++LGGG+VAD LLW+
Sbjct: 245 KDRLGYAPVVPLQEGIRRTIDSFSH----LTAGSQSKREGPSKASRILGGGKVADTLLWK 304
Query: 408 EEKKSFTCFLASFLLFYWFFLRGGTFISSVAQLLLCIVVVLYGYGFIPTNIYGFPVQKLS 467
+ K++ ++Y F G T ++++++ LL V L+ +G +P I+G+ V+K+
Sbjct: 305 DLKQTLIAIFILISIYYNFVATGSTVVTALSKALLVASVFLFLHGILPEKIFGYTVEKIP 364
Query: 468 ATMFGPSDTVVRDSIMGIACLWNRGVHNARSLARGEDWNYFLKAVTFLYVLKLFLSRSLT 527
A+ F S D + + WN V RSL +G DW++F K V L L L + SL
Sbjct: 365 ASQFHLSKDSSHDLSLSVISSWNTTVKALRSLCQGNDWSFFFKVVFVLLALSLAGAISLH 424
Query: 528 LLIGMGLVFAFTAFFVYEQYEIEIDGFAKFLFISIMELKSVLARH 569
+ +GL AF AF VYE+ E EID ++ KS +H
Sbjct: 425 SIFVIGLPIAFLAFLVYEKKEQEIDSI-------VVSFKSFACKH 458
BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match:
AT1G47290.2 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )
HSP 1 Score: 317.8 bits (813), Expect = 1.9e-86
Identity = 178/448 (39.73%), Postives = 263/448 (58.71%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
R CVV GGRGF R LV L++ ++VRIAD ++ L+P E+ +L +A+ +GR Y
Sbjct: 10 RWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYV 69
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+RNK Q+V G+ VVF+M DS N+ L Y VQG NVI+AC + V+RLI
Sbjct: 70 SADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLI 129
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+ SS V+FDG N +E L YP K D S KA+ EALIL AN GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPS 189
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
++FGP D +VP +V A++G +KFIIG+G N DFT+VENV HAH+CAE AL
Sbjct: 190 SIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVC 249
Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P ++ + ++ ++ G +
Sbjct: 250 AKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPY 309
Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKELSLAS 381
++L +C RTF+ A +LGYSP+V ++EGI TI+SFSHL+ +
Sbjct: 310 GMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ----- 369
Query: 382 LTEFTEESKADKLLGGGRVADILLWREEKKSFTCFLASFLLFYWFFLRGGT---FISSVA 441
+ K + V + + W+ K++ + L++ F G+ I++V+
Sbjct: 370 -----NQPKTE-------VTETIQWK--KQTLIAIVILITLYHNFVATTGSSSVIITAVS 429
Query: 442 QLLLCIVVVLYGYGFIPTNIYGFPVQKL 463
++LL + ++ G +P + F +K+
Sbjct: 430 KVLLVSSIFMFINGILPEKMKVFGSKKI 438
BLAST of Cp4.1LG08g05780 vs. TAIR 10
Match:
AT1G47290.1 (3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 )
HSP 1 Score: 317.4 bits (812), Expect = 2.5e-86
Identity = 163/355 (45.92%), Postives = 225/355 (63.38%), Query Frame = 0
Query: 22 RTCVVLGGRGFLGRSLVLRLLKLAKWNVRIADSVESLELDPSEQDSLLSDALVAGRASYF 81
R CVV GGRGF R LV L++ ++VRIAD ++ L+P E+ +L +A+ +GR Y
Sbjct: 10 RWCVVTGGRGFAARHLVEMLVRYQMFHVRIADLAPAIVLNPHEETGILGEAIRSGRVQYV 69
Query: 82 HVDVRNKDQIVAALSGSSVVFYMDYEDSHSNDIYLSYMFIVQGAKNVINACRDCKVRRLI 141
D+RNK Q+V G+ VVF+M DS N+ L Y VQG NVI+AC + V+RLI
Sbjct: 70 SADLRNKTQVVKGFQGAEVVFHMAAPDSSINNHQLQYSVNVQGTTNVIDACIEVGVKRLI 129
Query: 142 FNSSADVIFDGSRDIQNGEEPLAYPRKFEDMLSDLKAQAEALILFANDIDGLLTCALRPS 201
+ SS V+FDG N +E L YP K D S KA+ EALIL AN GLLTC +RPS
Sbjct: 130 YTSSPSVVFDGVHGTLNADESLPYPPKHNDSYSATKAEGEALILKANGRSGLLTCCIRPS 189
Query: 202 NVFGPEDTRLVPFVVNQAKSGLAKFIIGNGENLSDFTFVENVTHAHICAEEALDFR---M 261
++FGP D +VP +V A++G +KFIIG+G N DFT+VENV HAH+CAE AL
Sbjct: 190 SIFGPGDKLMVPSLVTAARAGKSKFIIGDGSNFYDFTYVENVVHAHVCAERALASGGEVC 249
Query: 262 VSVAGKAFFITNFKPMKFWEFISLILGGLGYRRPSVKLPFKMVWNVLLFLKWIHEKFG-F 321
AG+A+FITN +P+KFWEF+S +L GLGY RPS+K+P ++ + ++ ++ G +
Sbjct: 250 AKAAGQAYFITNMEPIKFWEFMSQLLEGLGYERPSIKIPASLMMPIAYLVELAYKLLGPY 309
Query: 322 SRYNHSTWARYIQLATCTRTFNCVAAHKQLGYSPIVSMEEGITLTIESFSHLRKE 373
++L +C RTF+ A +LGYSP+V ++EGI TI+SFSHL+ +
Sbjct: 310 GMKVPVLTPSRVRLLSCNRTFDSSKAKDRLGYSPVVPLQEGIKRTIDSFSHLKAQ 364
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
A9X4U2 | 8.6e-161 | 54.96 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3 OS=Arabidopsis thalia... | [more] |
Q67ZE1 | 2.2e-116 | 42.11 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 OS=Arabidopsis thalia... | [more] |
Q9FX01 | 2.6e-85 | 39.73 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 OS=Arabidopsis thalia... | [more] |
Q5PPL3 | 1.4e-57 | 37.68 | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Rattus norvegicus... | [more] |
Q9R1J0 | 4.0e-57 | 38.24 | Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating OS=Mus musculus OX=1... | [more] |
Match Name | E-value | Identity | Description | |
XP_023540745.1 | 0.0 | 100.00 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita pepo subsp. pepo] | [more] |
KAG6597621.1 | 0.0 | 98.97 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 3, partial [Cucurbita a... | [more] |
XP_022932574.1 | 0.0 | 98.80 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita moschata] | [more] |
XP_022972294.1 | 0.0 | 98.28 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase [Cucurbita maxima] | [more] |
XP_038897960.1 | 0.0 | 88.34 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase [Benincasa hispida] | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1EXD3 | 0.0 | 98.80 | Reticulon-like protein OS=Cucurbita moschata OX=3662 GN=LOC111439089 PE=4 SV=1 | [more] |
A0A6J1IB30 | 0.0 | 98.28 | Reticulon-like protein OS=Cucurbita maxima OX=3661 GN=LOC111470875 PE=4 SV=1 | [more] |
A0A0A0L5K0 | 0.0 | 87.48 | Reticulon-like protein OS=Cucumis sativus OX=3659 GN=Csa_3G174040 PE=4 SV=1 | [more] |
A0A1S3AXX4 | 0.0 | 87.65 | Reticulon-like protein OS=Cucumis melo OX=3656 GN=LOC103483967 PE=4 SV=1 | [more] |
A0A5A7VPP8 | 0.0 | 80.76 | Reticulon-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold5... | [more] |
Match Name | E-value | Identity | Description | |
AT2G43420.1 | 6.1e-162 | 54.96 | 3-beta hydroxysteroid dehydrogenase/isomerase family protein | [more] |
AT2G26260.1 | 1.6e-117 | 42.11 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 | [more] |
AT2G26260.2 | 1.2e-96 | 41.72 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 2 | [more] |
AT1G47290.2 | 1.9e-86 | 39.73 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 | [more] |
AT1G47290.1 | 2.5e-86 | 45.92 | 3beta-hydroxysteroid-dehydrogenase/decarboxylase isoform 1 | [more] |