Cp4.1LG08g04360 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG08g04360
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	SpoU_methylase domain-containing protein
Location	Cp4.1LG08: 1509651 .. 1513006 (-)
RNA-Seq Expression	Cp4.1LG08g04360
Synteny	Cp4.1LG08g04360

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAACGACACCGTATTGAAAAGAATTCATCAGACGCCCGGGCCAGGCTGAACTGTGAACCGCTCCTTCTTCTTCGATCCGCCGCCAGAACTGAGTTGCGGGCTCTGTCAAAACCCTCAATCCGTAAACCCCATCACTTTCTCTTCAATCATTTCATCGCCATTGAATTCGAAATGGGGTTCGAAAGCTACGTCGTCGTACATAACATAGCCAAAAGGCACAACGTTGGCACATTGGCCCGAAGCGCCACCGCGTTCGGTGTATCGGAGGTCATCCTCGTCGGCCGTCGGGACTTCAACGCCTTTGGTAGCCATGGTTCCACTTCTCACGTCCGGTTCCGTCATTTTCATTCCCTTCTCGACGCCCGAAATTTCCTTCAGGTTCTGCCCTCTTTCTTTCTTCCCTTCATCGGTCTCGATTAGGTTATGGGTTTGTTTTGAAATTTGAATTTTTTTCTGTCTGTGTCTGTAGGAGAAGGATTGTGATATATGTGGCGTGGAGATCACTGATAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACCGCTTTTCTTCTCGGCAACGAGGTTGGTTTTTGTGTTGGAATTCCATTTTTTATTGCATTTATTAATCACCATATGCTGCTGTCAGTTAATAAGTTCTTCTCTAATTCCATTATACATAGGACCTGTAGAGCTTATTGTCTCCCATTAGTTCTTTCATTTTTCTCAATTACAACTCGGTTTCTCACATATTGTGAGATCCCACATCGATGGGGGAAGTTAACAAAACGTTCTTTATAAGGGTGTGGAAACCTTTTCCTAGCAGACGCAAAGGAAAAGTCCAAAGAGGACAATATAGCGGTGGACTTAGGCCGTTACAAATAGTATGAGAGCTAGACACCGGGCGATGTACCAGCCAGGAGCCTAAGCCCTCGAAGGGTGTGGACATGAGACGATATGCCAGAAAGGACGCTGGGCCACGAAAGGAGGTGGATTGGAGGATCCCACATCGATTGGAGAAGGGAACGAGTGCTAGCAAGGATGGTAGACCCTAAAGGGGGTGGATTGTGAGATCTCACATCGGTTGGGGAGGAGAACGAAACACTCTTTATAAAGGTGTGGAAACCTCTTCCTAGCAGACACGTTTTAAAAATCTTGAGGGGAAGCTCGAAAGGGAAAGCCCAAAGAAGACAACATCTGCTAGTGGTGGGCTTGGAGGCGGCGATGAGTTTAATGCAAGGAGTGAACTCCGATTCCTTGATGAAAAAAATAAACGATAATTGCTTAAAGTTTCTCTGGCAATCATATTCTCTGTGATGGATTATTCTTCCATTTCTTGATGTGATGTCATTAGGATTTAATATGTCATTTTAGATCTCTTGCTTTTTTCTTAAGTCCATGTCTCTATGATGTTCTAGGCATTGTGAGTTAGTTCGTAGCATGATCATCAAGATTGAATTGGATCAGAATCATTTGGAAATAATTTTGATACACTAATTCAGCAAGAAGACACCCTTTTGATGTTTATATGCATATGATATTTCTAGGGAACAGGGCTTTCAGCTAAAGAGTGTGCAATCTGCGACTTCTTCATTTACATCCCACAATATGGTGTCGGCACCGCTTCTTTGAATGTCACTGTAGCTGCTTCAATTGTCCTACATCATTTTGGTGGTAATTAATACTCAGAACTGTTGCATTTTCATCTCCCCCTTTTGAGTTTCTTTTAAATACATGTCTAATCTCTATCACCTATGAGAATTACCAGTCTCTCTTAATGTTTTCGGTTTGATACGTTTGGTTTTTTACCATGTAAAATCATGAGTACTGTAAAATTACGTGATCGATGGAAGGACTTTAGGAAGAAATGCATATATGTCTTAAAAGTGTGTAAGGAGGGGCAGTTATCTGATCTGATTATTTTGATCCCTTTCTCTGAGTTCTGAATCAGTTCATTATGGGAAATGATCCCTTCAAGCTGAAACTTAGGAAACTGTGTCCTAGGAATTAGTATGGCTTGGTTGAATCAAATGATGTTGCAGTTTTTAATAGCTCTCATACTTGGTATTAACGATCATCTTGGATCGACATTAAGTATGCATGCTGTAATAGCCCAAGCCTACCGCTAGCAGATATTGTCATCTTTAGGCTTCTCCACAAGGTTTTTAAAACGCATCTACTAGGGAGAGGTTTCCATATCATTATAAAGAGTACTTCGTTCTCCTCCCTAACCGATGTGGGATCTCACAATCCACCCCCCTTTGGGGCCTAGCCTCCTCGCCGACACTCGTTCCCTTCTCCAATCAATGTGGGACCCCTCCAATTCACCCCCTTTTGGGGCCCAGCGTTCTTGCTGGCACACCGCCGTGTGTTCACCCCCTTTCGGGGCTTAGCTTCCTTGTTGGCACATCGCCCATTGTCTAGCTCTGATACCATTTGTAACAACCCAAGCCCCACTAGTAGATCCTGTTCTCTTTGGGCTTTCCCTTTCAGACTTCCTCTCAAGGTTTTTAAAACGCGTCTGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTTTCGTTCTTCTCCTATTATTGTATTAGGGTTCTTTCTCATGTGTGTATATTGGAGGTTTAAGTGAAAATCAGGTTGGTTATGCCTTATTTGATGAACTGGAAATGATACTTGTCCCCTTTTCTAGACATTATTGACCTCTCATCAACCATGTTTTTCAGTTTGGGCAGGCTTTCCCGAGCGATGTCGTGATGGTAACAAGTTTATAGTGGCCGAAAGACCAGTAAAGCAAGGCAAGCGGAATTATTGCCCTGAAACAGAAGAATCTATTGTTGAAGAGCGAAAGTCCAGGCGGGATAATGCTTCGAAAGGCTTCTTTGACGAGGGAACGATCGATGATTCGACATCCAACCTTCTTGATACATTGTTTATTAACGAATGAACGGAGTTGAAGCTGGGAGTCGTCATTCGACATCCAACCTTCTTGATACACAACCCACAGCAGACTGCATGATTATTGTAGCTCATTGCTGGAAGCTGTTCTGGAAAGAAAGAACTCCTTCTCCTTTGTCTTTATCTTTTTTTGTTCTTAGATTTCGATATTCCTAGAACGTTCGTGCAGTTATATTTAGTTAATTAGCTTGATGACTTACTCGGTCGTTTCGTCGAAAGGTACTTCCGACCACCACATTGTTAAGGCAAGTTTGTAAGAATAGGACAGAAAGCAAGCAGAAGGCAGAAGGATTTAAAGCAAAGGGTTGCAAGGTTGACTCAAACATGTTCTTCATTAGGGATGCCATTGATACATACTTAGATTACTTATACAAAAGTTATATCAGTTTGTACCATTTGGAAATTTTGGCTAAATCTTAT

mRNA sequence

AAACGACACCGTATTGAAAAGAATTCATCAGACGCCCGGGCCAGGCTGAACTGTGAACCGCTCCTTCTTCTTCGATCCGCCGCCAGAACTGAGTTGCGGGCTCTGTCAAAACCCTCAATCCGTAAACCCCATCACTTTCTCTTCAATCATTTCATCGCCATTGAATTCGAAATGGGGTTCGAAAGCTACGTCGTCGTACATAACATAGCCAAAAGGCACAACGTTGGCACATTGGCCCGAAGCGCCACCGCGTTCGGTGTATCGGAGGTCATCCTCGTCGGCCGTCGGGACTTCAACGCCTTTGGTAGCCATGGTTCCACTTCTCACGTCCGGTTCCGTCATTTTCATTCCCTTCTCGACGCCCGAAATTTCCTTCAGGAGAAGGATTGTGATATATGTGGCGTGGAGATCACTGATAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACCGCTTTTCTTCTCGGCAACGAGGGAACAGGGCTTTCAGCTAAAGAGTGTGCAATCTGCGACTTCTTCATTTACATCCCACAATATGGTGTCGGCACCGCTTCTTTGAATGTCACTGTAGCTGCTTCAATTGTCCTACATCATTTTGGTGTTTGGGCAGGCTTTCCCGAGCGATGTCGTGATGGTAACAAGTTTATAGTGGCCGAAAGACCAGTAAAGCAAGGCAAGCGGAATTATTGCCCTGAAACAGAAGAATCTATTGTTGAAGAGCGAAAGTCCAGGCGGGATAATGCTTCGAAAGGCTTCTTTGACGAGGGAACGATCGATGATTCGACATCCAACCTTCTTGATACATTGTTTATTAACGAATGAACGGAGTTGAAGCTGGGAGTCGTCATTCGACATCCAACCTTCTTGATACACAACCCACAGCAGACTGCATGATTATTGTAGCTCATTGCTGGAAGCTGTTCTGGAAAGAAAGAACTCCTTCTCCTTTGTCTTTATCTTTTTTTGTTCTTAGATTTCGATATTCCTAGAACGTTCGTGCAGTTATATTTAGTTAATTAGCTTGATGACTTACTCGGTCGTTTCGTCGAAAGGTACTTCCGACCACCACATTGTTAAGGCAAGTTTGTAAGAATAGGACAGAAAGCAAGCAGAAGGCAGAAGGATTTAAAGCAAAGGGTTGCAAGGTTGACTCAAACATGTTCTTCATTAGGGATGCCATTGATACATACTTAGATTACTTATACAAAAGTTATATCAGTTTGTACCATTTGGAAATTTTGGCTAAATCTTAT

Coding sequence (CDS)

ATGGGGTTCGAAAGCTACGTCGTCGTACATAACATAGCCAAAAGGCACAACGTTGGCACATTGGCCCGAAGCGCCACCGCGTTCGGTGTATCGGAGGTCATCCTCGTCGGCCGTCGGGACTTCAACGCCTTTGGTAGCCATGGTTCCACTTCTCACGTCCGGTTCCGTCATTTTCATTCCCTTCTCGACGCCCGAAATTTCCTTCAGGAGAAGGATTGTGATATATGTGGCGTGGAGATCACTGATAACGCCGTGGCTGTCAATCAGCATCCTTTCAAGAGGAGCACCGCTTTTCTTCTCGGCAACGAGGGAACAGGGCTTTCAGCTAAAGAGTGTGCAATCTGCGACTTCTTCATTTACATCCCACAATATGGTGTCGGCACCGCTTCTTTGAATGTCACTGTAGCTGCTTCAATTGTCCTACATCATTTTGGTGTTTGGGCAGGCTTTCCCGAGCGATGTCGTGATGGTAACAAGTTTATAGTGGCCGAAAGACCAGTAAAGCAAGGCAAGCGGAATTATTGCCCTGAAACAGAAGAATCTATTGTTGAAGAGCGAAAGTCCAGGCGGGATAATGCTTCGAAAGGCTTCTTTGACGAGGGAACGATCGATGATTCGACATCCAACCTTCTTGATACATTGTTTATTAACGAATGA

Protein sequence

MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHSLLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYIPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEESIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE

Homology

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy Swiss-Prot
Match: Q4L3J1 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=SH2477 PE=3 SV=1)

HSP 1 Score: 68.6 bits (166), Expect = 1.0e-10
Identity = 47/146 (32.19%), Postives = 71/146 (48.63%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + V++  +   HN+G++ R+A A GV  VI+  RR        A  S G+  HV   
Sbjct: 93  GLSTVVILDGLEDPHNLGSILRTADASGVDGVIIPKRRSVALTQTVAKASTGAIQHVPVM 152

Query: 62  HFHSLLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + L+E    + G E  DNA            A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKEHGYWVAGAE-ADNATDYRDMAADMPLAIVIGSEGQGMSRLVKDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy Swiss-Prot
Match: Q99W72 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) OX=158878 GN=SAV0531 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  VI+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + L++    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy Swiss-Prot
Match: Q7A794 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain N315) OX=158879 GN=SA0490 PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  VI+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + L++    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy Swiss-Prot
Match: Q6GJD7 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MRSA252) OX=282458 GN=SAR0535 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  VI+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + L++    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy Swiss-Prot
Match: Q6GBV6 (Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strain MSSA476) OX=282459 GN=SAS0489 PE=3 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.0e-10
Identity = 45/146 (30.82%), Postives = 72/146 (49.32%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFN-----AFGSHGSTSHVRFR 61
           G  + +++  +   HN+G++ R+A A GV  VI+  RR        A  S G+  HV   
Sbjct: 93  GLSTVLILDGLEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVI 152

Query: 62  HFHSLLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICD 121
              +L    + L++    + G E  +NA          S A ++G+EG G+S      CD
Sbjct: 153 RVTNLAKTIDELKDNGFWVAGTE-ANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCD 212

Query: 122 FFIYIPQYGVGTASLNVTVAASIVLH 143
           F+I IP  G    SLN +VAAS++++
Sbjct: 213 FYIKIPMVG-HVNSLNASVAASLMMY 236

[Close]

BLAST of Cp4.1LG08g04360 vs. NCBI nr
Match: KAG7028939.1 (tRNA (guanosine(18)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 445 bits (1144), Expect = 1.05e-157
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218

[Close]

BLAST of Cp4.1LG08g04360 vs. NCBI nr
Match: XP_022953920.1 (uncharacterized protein LOC111456233 isoform X2 [Cucurbita moschata])

HSP 1 Score: 445 bits (1144), Expect = 2.27e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 86  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 145

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 146 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 205

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 206 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 265

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 266 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 303

[Close]

BLAST of Cp4.1LG08g04360 vs. NCBI nr
Match: XP_022953849.1 (uncharacterized protein LOC111456233 isoform X1 [Cucurbita moschata])

HSP 1 Score: 445 bits (1144), Expect = 2.91e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 93  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 152

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 153 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 212

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 213 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 272

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 273 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 310

[Close]

BLAST of Cp4.1LG08g04360 vs. NCBI nr
Match: XP_023539188.1 (uncharacterized protein LOC111799908 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 445 bits (1144), Expect = 3.61e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 99  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 158

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 159 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 218

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 219 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 278

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 279 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 316

[Close]

BLAST of Cp4.1LG08g04360 vs. NCBI nr
Match: XP_022973638.1 (uncharacterized protein LOC111472230 isoform X2 [Cucurbita maxima])

HSP 1 Score: 445 bits (1144), Expect = 4.97e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 108 MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 167

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 168 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 227

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 228 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 287

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 288 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 325

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy TrEMBL
Match: A0A6J1GR02 (uncharacterized protein LOC111456233 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111456233 PE=4 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 1.10e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 86  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 145

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 146 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 205

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 206 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 265

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 266 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 303

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy TrEMBL
Match: A0A6J1GR06 (uncharacterized protein LOC111456233 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111456233 PE=4 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 1.41e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 93  MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 152

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 153 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 212

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 213 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 272

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 273 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 310

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy TrEMBL
Match: A0A6J1IDR0 (uncharacterized protein LOC111472230 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472230 PE=4 SV=1)

HSP 1 Score: 445 bits (1144), Expect = 2.40e-156
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 108 MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 167

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 168 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 227

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 228 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 287

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE
Sbjct: 288 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 325

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy TrEMBL
Match: A0A6J1I824 (uncharacterized protein LOC111472230 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472230 PE=4 SV=1)

HSP 1 Score: 435 bits (1119), Expect = 4.93e-152
Identity = 213/213 (100.00%), Postives = 213/213 (100.00%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS
Sbjct: 108 MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 167

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 168 LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 227

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE
Sbjct: 228 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 287

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDT 213
           SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDT
Sbjct: 288 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDT 320

[Close]

BLAST of Cp4.1LG08g04360 vs. ExPASy TrEMBL
Match: A0A1S3AXA1 (putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC103483771 PE=4 SV=1)

HSP 1 Score: 420 bits (1079), Expect = 4.16e-148
Identity = 203/218 (93.12%), Postives = 213/218 (97.71%), Query Frame = 0


Query: 1   MGFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHS 60
           MG ESYVVVHNIAKRHNVGT+ARSATAFGVSE+ILVGRRDFNAFGSHGSTSH+RFRHFHS
Sbjct: 1   MGIESYVVVHNIAKRHNVGTMARSATAFGVSELILVGRRDFNAFGSHGSTSHIRFRHFHS 60

Query: 61  LLDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120
           LLDA+ FL+EKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY
Sbjct: 61  LLDAQTFLKEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 120

Query: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 180
           IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPV+Q KRNYC ETEE
Sbjct: 121 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVRQVKRNYCAETEE 180

Query: 181 SIVEERKSRRDNASKGFFDEGTIDDSTSNLLDTLFINE 218
           SI+EERKSRR+NASKGFFDEG IDDSTSNLLDTLFI++
Sbjct: 181 SIIEERKSRRENASKGFFDEGMIDDSTSNLLDTLFIDQ 218

[Close]

BLAST of Cp4.1LG08g04360 vs. TAIR 10
Match: AT4G15520.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 358.2 bits (918), Expect = 4.6e-99
Identity = 172/220 (78.18%), Postives = 199/220 (90.45%), Query Frame = 0


Query: 2   GFESYVVVHNIAKRHNVGTLARSATAFGVSEVILVGRRDFNAFGSHGSTSHVRFRHFHSL 61
           GFESYVVVHNIAKRHNVGTLARSATAFGV+E+ILVGRRDFNAFGSHGS SH+RFRHFHSL
Sbjct: 3   GFESYVVVHNIAKRHNVGTLARSATAFGVTELILVGRRDFNAFGSHGSASHIRFRHFHSL 62

Query: 62  LDARNFL-QEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIY 121
           ++ARN+L +EKDCDICGVEI D A AVN+HPFKR+TAFLLGNEG+GLSAKE  ICDFF+Y
Sbjct: 63  IEARNYLKEEKDCDICGVEIADGASAVNEHPFKRNTAFLLGNEGSGLSAKEYEICDFFVY 122

Query: 122 IPQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEE 181
           IPQYG GTASLNVTVAASIVLHHFGVWAGF ER RDG+KFIVA+RPV+QG+RN+C  TEE
Sbjct: 123 IPQYGCGTASLNVTVAASIVLHHFGVWAGFSERVRDGSKFIVADRPVRQGRRNFCAGTEE 182

Query: 182 SIVEERKSRRDNASKGFFDEGTIDD--STSNLLDTLFINE 219
           S++EERK R+++A  GFFD+   ++  S+S+LLD LF+NE
Sbjct: 183 SVIEERKLRKESAENGFFDDNGNENGSSSSDLLDGLFLNE 222

[Close]

BLAST of Cp4.1LG08g04360 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 54.3 bits (129), Expect = 1.4e-07
Identity = 47/193 (24.35%), Postives = 81/193 (41.97%), Query Frame = 0


Query: 7   VVVHNIAKRHNVGTLARSATAFGVSEVILVG-----RRDFNAFGSHGSTSHVRFRHFHSL 66
           +VV  ++   N+    RSA A G+  V +V      R + N   S G+   +    + + 
Sbjct: 157 LVVEGLSDFGNISAAFRSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTP 216

Query: 67  LDARNFLQEKDCDICGVEITDNAVAVNQHPFKRSTAFLLGNEGTGLSAKECAICDFFIYI 126
            +    L+ +   I    +  + V++    +   TA ++GNEG G+S +   + D    I
Sbjct: 217 KECFKVLKSRGYRIATTHLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSI 276

Query: 127 PQYGVGTASLNVTVAASIVLHHFGVWAGFPERCRDGNKFIVAERPVKQGKRNYCPETEES 186
           P  G+   S NV+VAA I++HH                  V++R  + G      E E+ 
Sbjct: 277 PMNGM-VDSFNVSVAAGILMHH-----------------AVSDRTTRLGSHGDLSEAEKE 331

Query: 187 IVEERKSRRDNAS 195
           I+    S R + S
Sbjct: 337 ILMAEFSLRHSRS 331

The following BLAST results are available for this feature:

BLAST of Cp4.1LG08g04360 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4L3J1	1.0e-10	32.19	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus haemolyticus ...	[more]
Q99W72	3.0e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q7A794	3.0e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q6GJD7	3.0e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]
Q6GBV6	3.0e-10	30.82	Putative TrmH family tRNA/rRNA methyltransferase OS=Staphylococcus aureus (strai...	[more]

BLAST of Cp4.1LG08g04360 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028939.1	1.05e-157	100.00	tRNA (guanosine(18)-2'-O)-methyltransferase [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022953920.1	2.27e-156	100.00	uncharacterized protein LOC111456233 isoform X2 [Cucurbita moschata]	[more]
XP_022953849.1	2.91e-156	100.00	uncharacterized protein LOC111456233 isoform X1 [Cucurbita moschata]	[more]
XP_023539188.1	3.61e-156	100.00	uncharacterized protein LOC111799908 [Cucurbita pepo subsp. pepo]	[more]
XP_022973638.1	4.97e-156	100.00	uncharacterized protein LOC111472230 isoform X2 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG08g04360 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GR02	1.10e-156	100.00	uncharacterized protein LOC111456233 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GR06	1.41e-156	100.00	uncharacterized protein LOC111456233 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IDR0	2.40e-156	100.00	uncharacterized protein LOC111472230 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1I824	4.93e-152	100.00	uncharacterized protein LOC111472230 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A1S3AXA1	4.16e-148	93.12	putative TrmH family tRNA/rRNA methyltransferase OS=Cucumis melo OX=3656 GN=LOC1...	[more]

BLAST of Cp4.1LG08g04360 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15520.1	4.6e-99	78.18	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT5G15390.1	1.4e-07	24.35	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 3..149 e-value: 6.2E-39 score: 135.4
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 6..142 e-value: 2.2E-28 score: 99.2
None	No IPR available	PANTHER	PTHR43191:SF10	BNAA01G18460D PROTEIN	coord: 2..188
None	No IPR available	PANTHER	PTHR43191	RRNA METHYLTRANSFERASE 3,	coord: 2..188
None	No IPR available	CDD	cd18096	SpoU-like	coord: 5..144 e-value: 5.57617E-77 score: 225.943
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 5..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG08g04360.1	Cp4.1LG08g04360.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001510	RNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0003723	RNA binding
molecular_function	GO:0008173	RNA methyltransferase activity