Cp4.1LG08g02720 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG08g02720
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	cytochrome b5-like
Location	Cp4.1LG08: 2509521 .. 2512818 (-)
RNA-Seq Expression	Cp4.1LG08g02720
Synteny	Cp4.1LG08g02720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGATCTTAGAGATGGTGTCTGTGTTGTTGCACGTTGTTGCCAACCAATTGTGACCACTTTTGATTGGGTTTCCATTATTTTTTTGCATTTCAAACAAACACTATCGTCATTCCATCTCACCTACTACCGAATCCCAATTTTTTTTTTCTTTTTTTTTTTTTTGTAGTTGACATCCAAATCTTTCCGTGAGCTTAATTTCTCCTTTCAGTTATATATATATATATATATATATATATATATAGAGTATATAATTGTTTTACATTCGGTTTCCATCGTTGTTTTGTAGCCCAAAAGTTCCTTCTCTCTGCCCCCTCAATTCGATCGACGAACCCGATTCGAGCATATGGGTAGCGGAGAAAAGGTCTTCACCTTGAAGGAAGTCGCTGAGCACAACAATCACAAGGACTGTTGGCTCATCATCAGTGGCAAGGTATATATATATATATATATATAATCCATTTCTCTTCTTCTTCTTGGTCTTCTGGATCTATGATTTTTCCGTTTTACTCGCGGATTCTGCGAGTTTTTGGGTGGATTTTGGTTCTTGTTCTGGCGGTGGAGTTCGGCGTTGGTTTGGATTTTGTTTCAGATTTCAGATTTGTGTTGTTTTGTTTTGATTGAACTTGTTGATTTCGACTATAGATGTGGTTTTTGGCATTCTAATAGAGTATTGAACTGTTTAGATCTGTAGTTTCTGGACAGGATTTTATGATCTATTTGTTGTCTGTTATTCAACCTAATGGCTCTTGCTTGTAGTTACAGTGGTGATTTCATACTATTTCATACGAACATGGAGTCATTGATTGTCACTTAGGTTTTGTGCTAATTTTCAAACCAGTACAGTGTGGTATGAATTTCTTAGTTCTTTTTTCTTTTTGGTACTCTTATCTTCATATTGCTTGCTTTGTTTTCCTGATTATTAAGCTGTTTTGGACATTATCTTCTTGTCGATGAGACTTGAGTTTGTGAGCTTGGGACTTTACCAAAGAATATACAATGACATACANATTATCTTCTTGCCGATGAGACTTGAGTTTGTGAGCTAGGGACTTTACCAAAGAATATACAATTACATACAAAAAAAAACTAGGCCGAACTGAAGAACTATGAACGTTTACAGTATTTCGTCTAAATCTCACTCGAATGGGGAAATAATTTCCTTTACTAGATGATCTATGAATGGTTTACCGTTTTTCGTCTAAATCTCCCTCGAATGAGGTAATAATTTCCTTTACTAGAAGATTTATGGACAGTTTACAGTTTTTCGTCTAAACCTCACTTGAACGGGGAAATAATTTCCTTTACTAGAAAGTCTATGGATAGTTTACAGTTTTTCGTCGAAATCTCACTCAAACGGGGAAATAATTTCCTTTACTGGAACATCCATGGATAGTTTACAGTCTTTCGTCTAAATCTCCCTCGAACGAGGAACTAATTTCCTTTACTAGAAGATCTATGGATAGTTTACAGTTTTTCGTCTAAATCTCCTTCGAACGGGGAAATAATTTCCTTTATTAGAAGATGTACGGATAGTTTACAGTTTTTCATCTAAATCGCTCTTTCCTTTTTTAGAAGAACTATGGATAATGTACAGCTTGCCCGTTTCATACCCAGGTATCTCGTGTTACATCGTTGAAATTAACTGATATATAAGTTTATTTTCTTGATGAAAATTCATCAGAACTACCAACGATGATTAGTTTTGAGTTAGAGATAGCAATGATATGAAGATTTATAGGTAGAGAAGCTTAGTCTTTGATAATTTTGCCCCTTCCTAATATGGAGCTCATCATGGCTGTTCCATTGGCATATTTTTTATGATCTGGAAATCCTTCTACCATAATCTTTGGGGATTTTCTTTCGCTTTATGTTTTAATTCCATGGGGACGTCTTGGCAAAGGTACTCATTATTATGGTCGTTCATATGTTTGGAATGATCGGAATGCGTCGATGAATTCTTCGAACTTAAGAACTAGTGTATCCTACTTCGAACATTGGACAGTTCTTTACTTTAAAATGTTATTGTGTATGCAACTGATTTGGAGATTTGTACTGGATATTCTTTGGCATTCTTCATGCACAAAAGTAAATGATGTTAGTTGGAACTATGATATTCCAAACCTACTCCTAAGAGGGTAGTCCTAGTGGTAATGGCTCGGGATGTTTAAACGTTGAGATTACATTCCGAGGTCTTGGGTTAAATTCTTAGGCAGTCAGCTTAATCCTTAAAACTATTATGGTCAATCTTCGGAAATGTGAGGTCCCACGTTGGTTGGAGAGGGAACGAAACATTCCTTATAAGGGTGTAGAAAACCTCTCCCTAATAGATGCGTTTTAAAACCGTGAGGCCGACGGTGATACGTAATAGGCCACAGCGGATAATATTTGCTAGTGACGGGTTTAGGCTCTTACAGGAAGGGAAGAACCTGCTTTGACCTCTTGGTGAAGGAGTGCATGCATTAACGAGTTGAGCTATGCTTAGGATATGTGTTCGTTATAGAATCGTGTAGAATTATACCAGATTCTTTCAACTGAGTACACCAGCTTTCTGAACTCTTTGGTCCATTTGTTTATCTGAACAGGTATATGACGTGACCAAATTCTTAGACGACCATCCTGGAGGTGATGATGTTTTGTTATCAGCCACAGGTATGCTATTTCAGCTTATAGAGTTTCAATCCTAACCCACTGTCCATAATTTGTACTTTTCGAAACAACAACACCTAACGTTTACACGGTGCGATCCCTCGATTTTTCTCGATCAGGAAAAGATGCAACGGACGATTTTGAAGATGTGGGGCACAGTGACAACGCAAGAGAAATGATGGATCAATACTATGTTGGAGAGATTGATGGTTCGACAATTCCAAAGAAGGTCGCATACACGCCTCCGAAACAGCCTCATTACAATCAGGACAAGACGTCGGAGTTCATCATCAAGCTCCTCCAGTTCCTCGTCCCGCTTGCAATTTTGGGATTGGCAGTTGCCTTACGTTTCTACACGAAACAAGCTTGAGGTTGGTTGGTACCTTAAAACTTTTAAATTTGTTAGATTGGTAGTAGTTGATTTTTATGCTGTTTGCTAATGATCATTTGGTTACGTTGTTTTCTTGGTTATATAATGCGAGACAATCACAAGTGTTTTCAGTTTCCAACTTACTCTAGATTTGTCATATTTAATGTACCTCTATACTTAATTTCAAATAAAGATGGTCGAGATTTCGTTATCTGCTCGGTAGCTGACCTCAATCGAGATCAACAACTGTTCGTTCGTTGATTCTCAGTGAAACCTCGGTTT

mRNA sequence

GGATCTTAGAGATGGTGTCTGTGTTGTTGCACGTTGTTGCCAACCAATTGTGACCACTTTTGATTGGGTTTCCATTATTTTTTTGCATTTCAAACAAACACTATCGTCATTCCATCTCACCTACTACCGAATCCCAATTTTTTTTTTCTTTTTTTTTTTTTTGTAGTTGACATCCAAATCTTTCCGTGAGCTTAATTTCTCCTTTCAGTTATATATATATATATATATATATATATATATAGAGTATATAATTGTTTTACATTCGGTTTCCATCGTTGTTTTGTAGCCCAAAAGTTCCTTCTCTCTGCCCCCTCAATTCGATCGACGAACCCGATTCGAGCATATGGGTAGCGGAGAAAAGGTCTTCACCTTGAAGGAAGTCGCTGAGCACAACAATCACAAGGACTGTTGGCTCATCATCAGTGGCAAGGTATATGACGTGACCAAATTCTTAGACGACCATCCTGGAGGTGATGATGTTTTGTTATCAGCCACAGGAAAAGATGCAACGGACGATTTTGAAGATGTGGGGCACAGTGACAACGCAAGAGAAATGATGGATCAATACTATGTTGGAGAGATTGATGGTTCGACAATTCCAAAGAAGGTCGCATACACGCCTCCGAAACAGCCTCATTACAATCAGGACAAGACGTCGGAGTTCATCATCAAGCTCCTCCAGTTCCTCGTCCCGCTTGCAATTTTGGGATTGGCAGTTGCCTTACGTTTCTACACGAAACAAGCTTGAGGTTGGTTGGTACCTTAAAACTTTTAAATTTGTTAGATTGGTAGTAGTTGATTTTTATGCTGTTTGCTAATGATCATTTGGTTACGTTGTTTTCTTGGTTATATAATGCGAGACAATCACAAGTGTTTTCAGTTTCCAACTTACTCTAGATTTGTCATATTTAATGTACCTCTATACTTAATTTCAAATAAAGATGGTCGAGATTTCGTTATCTGCTCGGTAGCTGACCTCAATCGAGATCAACAACTGTTCGTTCGTTGATTCTCAGTGAAACCTCGGTTT

Coding sequence (CDS)

ATGGGTAGCGGAGAAAAGGTCTTCACCTTGAAGGAAGTCGCTGAGCACAACAATCACAAGGACTGTTGGCTCATCATCAGTGGCAAGGTATATGACGTGACCAAATTCTTAGACGACCATCCTGGAGGTGATGATGTTTTGTTATCAGCCACAGGAAAAGATGCAACGGACGATTTTGAAGATGTGGGGCACAGTGACAACGCAAGAGAAATGATGGATCAATACTATGTTGGAGAGATTGATGGTTCGACAATTCCAAAGAAGGTCGCATACACGCCTCCGAAACAGCCTCATTACAATCAGGACAAGACGTCGGAGTTCATCATCAAGCTCCTCCAGTTCCTCGTCCCGCTTGCAATTTTGGGATTGGCAGTTGCCTTACGTTTCTACACGAAACAAGCTTGA

Protein sequence

MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFEDVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAVALRFYTKQA

Homology

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BLAST of Cp4.1LG08g02720 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 2.5e-60
Identity = 108/132 (81.82%), Postives = 121/132 (91.67%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MG   K+FTL EV+EHN   DCW++I+GKVY+VTKFL+DHPGGDDVLLS+TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHS++AREMM+QYYVGEID +TIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTK 133
           LGLAV +R YTK
Sbjct: 121 LGLAVGIRIYTK 132

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.6e-59
Identity = 105/134 (78.36%), Postives = 119/134 (88.81%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MG   KVFTL EV++HNN KDCWL+ISGKVYDVTKFLDDHPGGD+VLLSATGKDATDDFE
Sbjct: 1   MGGETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHS +AR M+D+YYVG+ID +TIP K  YTPP QPHYNQDKTSEF++KLLQFLVPL I
Sbjct: 61  DVGHSSSARAMLDEYYVGDIDSATIPTKTKYTPPNQPHYNQDKTSEFVVKLLQFLVPLII 120

Query: 121 LGLAVALRFYTKQA 135
           LG+A  +RFYTKQ+
Sbjct: 121 LGVAFGIRFYTKQS 134

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 5.3e-58
Identity = 104/134 (77.61%), Postives = 118/134 (88.06%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MG   KVFTL EV+ HNN KDCWLIISGKVY+VTKFL+DHPGG +VLLSATGKDATDDFE
Sbjct: 1   MGGQSKVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           D+GHS +AR M+D+YYVG+ID STIP KV YTPPKQPHYNQDKT+EFI+KLLQFLVPL I
Sbjct: 61  DIGHSSSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYNQDKTTEFIVKLLQFLVPLII 120

Query: 121 LGLAVALRFYTKQA 135
           LG+A  + FYTKQ+
Sbjct: 121 LGVAFGVHFYTKQS 134

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy Swiss-Prot
Match: P49100 (Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2)

HSP 1 Score: 217.2 bits (552), Expect = 1.1e-55
Identity = 101/128 (78.91%), Postives = 115/128 (89.84%), Query Frame = 0


Query: 5   EKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFEDVGH 64
           +KV+TL+EVA+HN+  DCWLII GKVY+V+KFL+DHPGGDDVLLS+TGKDATDDFEDVGH
Sbjct: 6   KKVYTLEEVAKHNSKDDCWLIIGGKVYNVSKFLEDHPGGDDVLLSSTGKDATDDFEDVGH 65

Query: 65  SDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLA 124
           S  AR MMD+YYVG+ID STIP +  Y PPKQPHYNQDKT EFIIK+LQFLVPLAILGLA
Sbjct: 66  STTARAMMDEYYVGDIDTSTIPARTKYVPPKQPHYNQDKTPEFIIKILQFLVPLAILGLA 125

Query: 125 VALRFYTK 133
           VA+R YTK
Sbjct: 126 VAIRIYTK 133

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy Swiss-Prot
Match: O04354 (Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1)

HSP 1 Score: 215.3 bits (547), Expect = 4.2e-55
Identity = 98/129 (75.97%), Postives = 114/129 (88.37%), Query Frame = 0


Query: 6   KVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFEDVGHS 65
           K+FTL EVA+HNN KDCWLII+GKVYDVTKFL+DHPGGDDVLLSATGKDATDDFED+GHS
Sbjct: 3   KIFTLAEVAQHNNSKDCWLIINGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFEDIGHS 62

Query: 66  DNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAILGLAV 125
            +A+ M+D+YYVG+ID S+IP +V YTPPKQP YN DKT EF+IKLLQFLVPL IL  A+
Sbjct: 63  SSAKAMLDEYYVGDIDSSSIPSQVKYTPPKQPLYNPDKTREFVIKLLQFLVPLVILAGAI 122

Query: 126 ALRFYTKQA 135
            +RFYTK +
Sbjct: 123 GIRFYTKSS 131

[Close]

BLAST of Cp4.1LG08g02720 vs. NCBI nr
Match: XP_022936377.1 (cytochrome b5-like [Cucurbita moschata] >XP_022974878.1 cytochrome b5-like [Cucurbita maxima] >XP_023539499.1 cytochrome b5-like [Cucurbita pepo subsp. pepo] >KAG7028761.1 Cytochrome b5 isoform B [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 276 bits (706), Expect = 1.11e-93
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVALRFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. NCBI nr
Match: XP_023521594.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 276 bits (705), Expect = 1.58e-93
Identity = 133/134 (99.25%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGST+PKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTVPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVALRFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. NCBI nr
Match: XP_008438367.1 (PREDICTED: cytochrome b5 [Cucumis melo] >XP_038884390.1 cytochrome b5-like [Benincasa hispida] >KAA0049155.1 cytochrome b5 [Cucumis melo var. makuwa] >TYK17406.1 cytochrome b5 [Cucumis melo var. makuwa])

HSP 1 Score: 271 bits (694), Expect = 7.51e-92
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDASTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. NCBI nr
Match: XP_022147043.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 271 bits (693), Expect = 1.07e-91
Identity = 130/134 (97.01%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNH+DCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHRDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVTYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. NCBI nr
Match: XP_004133998.1 (cytochrome b5 [Cucumis sativus] >KAE8650304.1 hypothetical protein Csa_009551 [Cucumis sativus])

HSP 1 Score: 271 bits (694), Expect = 7.35e-91
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 68  MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 127

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 128 DVGHSDNAREMMDQYYVGEIDSSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 187

Query: 121 LGLAVALRFYTKQA 134
           LGLAVA+RFYTKQA
Sbjct: 188 LGLAVAIRFYTKQA 201

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy TrEMBL
Match: A0A6J1IHM0 (cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111473649 PE=3 SV=1)

HSP 1 Score: 276 bits (706), Expect = 5.37e-94
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVALRFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy TrEMBL
Match: A0A6J1F8A2 (cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111443010 PE=3 SV=1)

HSP 1 Score: 276 bits (706), Expect = 5.37e-94
Identity = 134/134 (100.00%), Postives = 134/134 (100.00%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVALRFYTKQA
Sbjct: 121 LGLAVALRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy TrEMBL
Match: A0A5D3D383 (Cytochrome b5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002660 PE=3 SV=1)

HSP 1 Score: 271 bits (694), Expect = 3.64e-92
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDASTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy TrEMBL
Match: A0A0A0L7Y9 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G131950 PE=3 SV=1)

HSP 1 Score: 271 bits (694), Expect = 3.64e-92
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDSSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. ExPASy TrEMBL
Match: A0A1S3AWT9 (cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103483485 PE=3 SV=1)

HSP 1 Score: 271 bits (694), Expect = 3.64e-92
Identity = 131/134 (97.76%), Postives = 133/134 (99.25%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFL+DHPGGDDVLLSATGKDATDDFE
Sbjct: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLEDHPGGDDVLLSATGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSDNAREMMDQYYVGEID STIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSDNAREMMDQYYVGEIDASTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTKQA 134
           LGLAVA+RFYTKQA
Sbjct: 121 LGLAVAIRFYTKQA 134

[Close]

BLAST of Cp4.1LG08g02720 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 232.6 bits (592), Expect = 1.8e-61
Identity = 108/132 (81.82%), Postives = 121/132 (91.67%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MG   K+FTL EV+EHN   DCW++I+GKVY+VTKFL+DHPGGDDVLLS+TGKDATDDFE
Sbjct: 1   MGDEAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHS++AREMM+QYYVGEID +TIPKKV YTPPKQPHYNQDKTSEFIIKLLQFLVPLAI
Sbjct: 61  DVGHSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120

Query: 121 LGLAVALRFYTK 133
           LGLAV +R YTK
Sbjct: 121 LGLAVGIRIYTK 132

[Close]

BLAST of Cp4.1LG08g02720 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 201.8 bits (512), Expect = 3.4e-52
Identity = 92/133 (69.17%), Postives = 112/133 (84.21%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           M S  KV + +EV++HN  KDCWLIISGKVYDVT F+DDHPGGD+VLLS+TGKDAT+DFE
Sbjct: 1   MSSDRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQFLVPLAI 120
           DVGHSD AR+MMD+Y++GEID S++P    Y  P+QP YNQDKT EFIIK+LQFLVP+ I
Sbjct: 61  DVGHSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPEFIIKILQFLVPILI 120

Query: 121 LGLAVALRFYTKQ 134
           LGLA+ +R YTK+
Sbjct: 121 LGLALVVRHYTKK 133

[Close]

BLAST of Cp4.1LG08g02720 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 190.3 bits (482), Expect = 1.0e-48
Identity = 87/134 (64.93%), Postives = 109/134 (81.34%), Query Frame = 0


Query: 1   MGSGEKVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFE 60
           MG   KVFTL EV++H++ KDCW++I GKVYDVTKFLDDHPGGD+V+L++TGKDATDDFE
Sbjct: 1   MGGDGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFE 60

Query: 61  DVGHSDNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPH--YNQDKTSEFIIKLLQFLVPL 120
           DVGHS  A+ M+D+YYVG+ID +T+P K  + PP        QDK+S+F+IKLLQFLVPL
Sbjct: 61  DVGHSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPL 120

Query: 121 AILGLAVALRFYTK 133
            ILGLA  +R+YTK
Sbjct: 121 LILGLAFGIRYYTK 134

[Close]

BLAST of Cp4.1LG08g02720 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 134.8 bits (338), Expect = 5.1e-32
Identity = 63/129 (48.84%), Postives = 91/129 (70.54%), Query Frame = 0


Query: 7   VFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFEDVGHSD 66
           + +  +VA+H    DCW++I GKVYD++ F+D+HPGGD+VLL+ TGKDA+ DFEDV HS 
Sbjct: 4   LISFHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSK 63

Query: 67  NAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTS--EFIIKLLQFLVPLAILGLA 126
           +A+E+M +Y +G++D ST+P    Y PP +      +T+  E   KLL +L+PL ILG+A
Sbjct: 64  DAKELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVA 123

Query: 127 VALRFYTKQ 134
            ALRFY  +
Sbjct: 124 FALRFYNNK 132

[Close]

BLAST of Cp4.1LG08g02720 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 130.6 bits (327), Expect = 9.6e-31
Identity = 58/127 (45.67%), Postives = 93/127 (73.23%), Query Frame = 0


Query: 6   KVFTLKEVAEHNNHKDCWLIISGKVYDVTKFLDDHPGGDDVLLSATGKDATDDFEDVGHS 65
           K+++++E A HN   DCW++I GKVYDV+ ++D+HPGGDDVLL+  GKDATDDFED GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 66  DNAREMMDQYYVGEIDGSTIPKKVAYTPPKQPHYNQDKTSEFIIKLLQ-----FLVPLAI 125
            +ARE+M++Y++GE+D S++P+      P+   Y +D+  + + KL       ++VP++I
Sbjct: 66  KDARELMEKYFIGELDESSLPE-----IPELKIYKKDQPQDSVQKLFDLTKQYWVVPVSI 125

Query: 126 LGLAVAL 128
           + ++VA+
Sbjct: 126 ITISVAV 127

The following BLAST results are available for this feature:

BLAST of Cp4.1LG08g02720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O48845	2.5e-60	81.82	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49098	1.6e-59	78.36	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49099	5.3e-58	77.61	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P49100	1.1e-55	78.91	Cytochrome b5 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0108800 PE=2 SV=2	[more]
O04354	4.2e-55	75.97	Cytochrome b5 OS=Borago officinalis OX=13363 PE=2 SV=1	[more]

BLAST of Cp4.1LG08g02720 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022936377.1	1.11e-93	100.00	cytochrome b5-like [Cucurbita moschata] >XP_022974878.1 cytochrome b5-like [Cucu...	[more]
XP_023521594.1	1.58e-93	99.25	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_008438367.1	7.51e-92	97.76	PREDICTED: cytochrome b5 [Cucumis melo] >XP_038884390.1 cytochrome b5-like [Beni...	[more]
XP_022147043.1	1.07e-91	97.01	cytochrome b5-like [Momordica charantia]	[more]
XP_004133998.1	7.35e-91	97.76	cytochrome b5 [Cucumis sativus] >KAE8650304.1 hypothetical protein Csa_009551 [C...	[more]

BLAST of Cp4.1LG08g02720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IHM0	5.37e-94	100.00	cytochrome b5-like OS=Cucurbita maxima OX=3661 GN=LOC111473649 PE=3 SV=1	[more]
A0A6J1F8A2	5.37e-94	100.00	cytochrome b5-like OS=Cucurbita moschata OX=3662 GN=LOC111443010 PE=3 SV=1	[more]
A0A5D3D383	3.64e-92	97.76	Cytochrome b5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G002660...	[more]
A0A0A0L7Y9	3.64e-92	97.76	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]
A0A1S3AWT9	3.64e-92	97.76	cytochrome b5 OS=Cucumis melo OX=3656 GN=LOC103483485 PE=3 SV=1	[more]

BLAST of Cp4.1LG08g02720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G32720.1	1.8e-61	81.82	cytochrome B5 isoform B	[more]
AT5G53560.1	3.4e-52	69.17	cytochrome B5 isoform E	[more]
AT5G48810.1	1.0e-48	64.93	cytochrome B5 isoform D	[more]
AT2G46650.1	5.1e-32	48.84	cytochrome B5 isoform C	[more]
AT1G26340.1	9.6e-31	45.67	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 68..80 score: 40.09 coord: 40..54 score: 55.56 coord: 30..40 score: 68.6 coord: 55..62 score: 48.11
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 8..81 e-value: 1.6E-35 score: 134.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 9..80 e-value: 5.0E-28 score: 97.0
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 5..81 score: 30.779724
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 3..84 e-value: 8.6E-37 score: 127.1
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 6..91
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 1..132
None	No IPR available	PANTHER	PTHR19359:SF78	CYTOCHROME B5	coord: 1..132
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 36..43

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG08g02720.1	Cp4.1LG08g02720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043447	alkane biosynthetic process
biological_process	GO:0042761	very long-chain fatty acid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding