Cp4.1LG08g02160 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG08g02160
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	3-hydroxyisobutyryl-CoA hydrolase 1-like
Location	Cp4.1LG08: 2912868 .. 2924288 (-)
RNA-Seq Expression	Cp4.1LG08g02160
Synteny	Cp4.1LG08g02160

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAGCCATAATCCTCGCTTGGAAGAATGGCGGCAAACTGAATCGTTAGCTATTTAGCTTCCACACTCATCTCTTTCCTATTTCATTTTGTTGAACCAGAGGTAGCATCTGCGAGCAATGGCGTCCTGGAATTCTACCGGAATTGATGAAGATCAGGTCTCTCTCTCGCTCTCTTCATCTCTATTTATCGTGCTTGTACAGATTGAACAATGGCCGTTTTTCTGAAGCTATTGAAATCCATGTCGAACTCGGCTAGGATTTGTAATTTCATTAGCTTTTAAGTGATAGAGTTTTCTCACGAATCTGACGGATAATCCGTTATCACATTGAAGCTCTTTTGACGTTTGTTGTTTATGTTTGTTCGTTAGCTGTAATTTTAAGTAAAGGAGGCTATAGATACTGAAATTTTTCTGAGACTTTAGAATCGTCGTACAAAATATGGTGCTTATTAGCTTAGTTGTTTTCTATGGAGATGTTGATGATCATACATTTTTCAGATGTTAATTTCTTTGCAGCAGGTTTTAGTGGAAGAGAAGCGGTTTGCGAGGATATTGACTTTAAACAGACCTAAGCAGTTAAATGCTATCTCATTCACAATGGTGAGGCTTGCGATATCTCCACTTTAAGCGTAGTCTCGTGATTTTCTAATGACGTTAATTTTATCTAAGTTCGAGAAAATTGTCGGTCTATTTGTTACTTCAGTCATGGATTAGCAATAATTGGACTATCTGGTCCTTGCTGGTTAACAAATTATATTCCATAAGCACCGAATTCTTGGGCTCAAGAACTCTGTATCTTGTAATTTACAAGCATTATGTGTCGCAATGTGAGTTTTGACAAGTCTGATGCCCGTGGTTTAAAATTCTATCTCCAAGATTTGGGTAGAATCGACAAAGCAATGGGAGTGTTCATAACTGAGAAACTAGATAAACGCTCTGTTTAGTGTTTTTCTTTTATCATTCAATCCAGGTTTCGCGGCTATTGGAACTCTTTTCTTCCTATGAGACAGATCCCAGTGTGAAACTTGTGGTTGTCAAGGTATTTAACTTCTCCATCAACAATTTTTATATATTGCTGTCATGTCCATTTTATCTTATTGTTCACCTACTGCTTCCATCGTTATCTTGTTCAAATGGAATTGTTCAATTACCTTAATGGAAAATATTGGAGGCGAAAGCATGACACTTACAAAGGGATCCTGTCCATGCCATGTTCTAAACCTTAACTGACCATGCCACCATGAAATTTTCGGAAGACAAGAGCGTTATTTATTTTTTAATTTCGTACTTAGCTGTGTAAAGTTCTTATCTTATCCATTTCAATACTAAATTCAAACAAGTGGGATCTTCATATTTTATCAAAACCAAGTAATAAAATTACCCAGATCACCCAACATTTAGCCCACATAGCATGCAAATTCAGTCACAAAAACAGTCTAAAATGAAGATTTCCCTCGTTATCATTATCCTTGTTGGTTTTCTTTGGGTTTCTTTGCATCCCACCTTTGTAGGTAAGAATGAGAATGATGATTTGATGAATTATATTCTAGTTGATGAGTTGGTTTATTATGATAGTCCGTAGAATGATCAATTAATACTAGAAAATGCTGTTTATTACACAGTATAATGAAGGCTACTGATGTAAATTACAATATCAAGGGCATGATCATTTGTTCTGTTTTCTATGAAGCATTTGGTCGCAGTTATATTTAACCGTTTCCCTCTTCTTATGATTCTCAGTGTATTTACTGATAATAAATAATTTGTGAATTATTAGGGTAAAGGAAGAGCATTTTGTGCTGGGGGTGATGTATCGGCTGCGGCTCATGCCATTAAAGAGAGTAAGTTTCTCTTTACTGCACAATCCACAGCGTCAATTAATCCCGAGCACCTAATGGATGCTTGAGACAGAGGATTTTACATTTTTTTTTTACATGTCTGTCATCTAGTCTAGGATAGCTGTGCAGAAGTTAACTTGATAATACACAACAGCATTTATATTTAGCTAATCATAATCCTGGTTTCTGTGAGTTAGGTGAATGGAGGAAAGGTGCTCACTTCTTCCAGAAACAATACACCTTAAATTATTTGTTGGCAACTTTCAGTAAGCCACAGGTAATTTTGGTTGCTGAAGTTCTGGTCGTTTTAGTCTATAATGACTTTTACTTGATATTATGTTTAACTTCCATTGATTGCTTGAATATTTTTCAAGGTTTCTCTTCTGAATGGTATTGTCATGGGAGGAGGAGCCGGTGTTTCAATACATGGTAGATTCCGTGTTGCAACAGAGAAATCGGTATATTATCTAACTGAAGCTCTGAACTTTTGGTCCTTGAATTATTGATCTAGTGCCCACCTTTTACAGTTTAAATTTAGAATTTTGTATTCTTCACCCTCATTTTTATTTATCTTTTAAGTAGCGAAATTTAGAACATGGATAATATCTTCAATTTTTGGCTTTCCTAAAATATTTTACTTGCTTCTTCATTGGAATAATATTTAGTAAGTAAGCTGCAAAAAGAGTGTTCTTTTTCTCTCTTTCTTCTCTAAAGAGTTGCTAATTTAGCTCTTTAAAGAATTTATTGGTAGGGACAAAAAGTAGAGCTTTTGAAGTTGTGTAGAATGCTTGGAAGAGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNGTGTAGATTTAAAAATTTAAGGATGTCGAAATATCATTCTACTCTGGTACTTATATACTGTAGGTGGAGTTTGTTTCTGTTTCTTCCATTCTTCAATCTCATTAGGAAAAAGGGAATACATTAATATGGCAACTTACCTTTTGCAGATTTTTGCAATGCCCGAAACTTCTTTAGGACACTTCCCAGATGTTGGTGCTTCTTATTACTTGCCAAGACTCCGGGGATTTTTTGGTATTACTAATTATCCTAACCTAGACACTCCTTTTTCCAGTGTGTAAGTGTATGTATTTTTTAATACTGAGCCTTAGTAGACATGCACATCAAAGCATACACTATGGATAATTTTACTCACTGACCAAACAAAGCAAGCGTCCTCCTTAGATGAGCTCGTGGCTTAGTCAAGTTGAACCAAGTTGCATTTGTGTCCCCACTTTCCTCTTGAGATTCATTTTGGTTATAGCTAGGTACAGAACGATCCAGCAATAAAGTATTTCTTTGTCAACTTGAAGTTGAAGTGAGAAGAGAATGAAAGGGATAGGTTCCATTGGCAAGTACAAATTGATCATTAACCTCGAAAGAGGCATCAGTTAAGTTGCCTCAATATCCAAATTCAACTTGTTCCCTGGTTCCAGTCAGAGACTGCAGTGGCTTCTCTTGTTCTTTCAACCATTTTTCCATTTCAATTATGGTGTAGAGAAGAAATCATTATTTGCCCTCGCTATTATCTTTTCAACCTCCATTTGATTTCAGTATAGAGAATGACAATTGTAGTTCACTGGTTTAATTGAATGCTTGATTTTTAGGGATGATTTCTTATGTTGCTCGAACCAGCTGGAATATAGAGGAAATGAACAGACTTTATCTTGGCTAAGAGCATTTCCAATTCTCCACCCTACCCTAAAATATTTTCCATCTGATGGTTAATGTAGCGAAAATTCCTTGTGCTTGGAGGTACTCTATTCTATTTATTATGTCTTATTCTTTTGCTAAAACTTTTGCAGGCGAATATGTTGGCCTAACAGGTGCCAGGTTGGATGGTGCTGAAATGCTTGCATGTGGCCTTGCTACTCACTTTGTCCCTTCAGAGGTATACTAACTCCTAAATTTCTATGATGCTCAAATGTTTAGCAGCAAAAAAGAAAGATATCCCCTCTTTAGGTGAAGAACTGTTTCAAGTAAGTTATAATATTATATAGGTTTGTTGACAACACGTCATAGATTATAAGGTGGTTGTTTCCTAGCATTAGTTGAGGTATATATCAGACATTTGATACCCTTGGTTATTTCAAAGATAAAAATGGTTGATTGCTTGGAGATAATAATTTGCAGTTATACCATTTTTTGTTTGATTTATACCATATTATGTACGTGGGATGTGTTCCTCAGTACCCTACATCTCAGGTGTTTTCTCTTAATCTCGAGCTTCTAGTATTTTATTTAAGAGAGAATAACTTCCTCTTCCTTTTTGTTACTCCAATCAATGACCAGAGATTAACGCAATTAGAGGAAGCACTATGCAGAGCAGATACAAGTCAACCAAGCATCATCTCTGCAATTATTGATAAATTCTCCCAGACACCAATTCTTCACGAGCAAAGTGCTTATAACCGGTGTGACTCCATCTTTGATTTATTGTTTTTTCCTTGAGCGCTAGTACCAAATTTTCTTAATGCAAAAATTCTAGGCAATGATTTTATCACCTTGAATATTTATATTTATAGTGTTTCCTATCAAAATCCTTTTGAGCTGAGTAATGTCCTATTGAGCTCTTCCTAGTTAAAAATTTTTTGTATAAATTTCAAAAGAACATTTTATTCCCACCTTTTTTAAAACTACCATCCACCTAAAAGTAATCAATCCCTTCACACTATTATTGTAATATATCTTCATGTTTCCAACTTGTTAAATTTGACAGATTTAGAAGTTCAATTTTAAAGAAAATGTGGTTCTCAAACGCAGTGAACTTCCGTGTTGTAACAGGTTGGATGTTATTGATAAGTGCTTCTCCCGAAGAACAGTAGAAGACATTTTGGCAGCTCTTGTAAGCTGATGTGGAGAACACAACATCTTCTTGCCGCTTGTGTTCTTTATAATTTAACTCGCATTTTCTTATTTTTCTTGTAGTTCTTTGGATATTGCATGAGCATGGTCATTGGAGGGATCGTTTCATTTAACCAATTGTAATCTGATGCTTTGCTCTTGTAGGAAGAGGAGGCCCTGAAAAAGAAAGATAATTGGATCAATTCAACAATTCGAGACTTGAAGAAGGCATCACCAACAAGTCTTAAAATCTCTTTGAGATCGGTGTGTAACAATTCTAACAAGTTTCGAACAGTCTCTCTCTTTGAAGGACTGCTGCACAGGATATGAATTTTGATGATTGACAGCCTTAGTTATTGGGATTCCTTCAACACATATGTGCGGGCAACTGCTGTCACATTTTTTTGTTGAAAGCGGTTGTTAGGTTGATTACACCATTTAGAGTCTTAGATCTATTGAGAATTGTTGAGAGGGAGTCCCACATTGGCTTATTTAGGAAATGATCATGGTTATAAGTACGGAATACATCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAGTCAAACCCACGAGAGCTTATGCTCAAAGTGGACAATATCAATACCATTGTGGAGATTCGTAATTCCTAACAAGGTCTATGCCTCCTCGGTAAAACTAGAAAATATCAGAGTGATCTACATGTATGGGGCACGATAGGGAAATCCTTGAAGGCAAAATGCCAAGGGGGAGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANTGAGTAGTTTTGAGATGATTTAGATACAGCATTCCAAGAGAAAGCAATAAGAAGAACTAACTTCCAAATATCATGTGACCTTTCATTCTCTAAAACATTCTCTCCTCTTTCTTGCCACCAAAATTTTGAAATTTCCATCAATGAACTTACTATGGTCTTTTTCTGGAATGACAGATAAGAGAAGGCAGACTTCAAGATGCTGGTCAGTGTCTCATTCGTGAGTATAGAATGGCCTGTCATGTCATGCGAGGGGAAGTCAGCGAAGATTTCATTGAGGTCTTCCACTGACTTTTATCTCCACATTTTTTACATTTTTTTGTGAGATCTCACATAGGTTGAGGAGGAGAACGAAACATTCTTTATAAGAGTGTGGAAACCTCTCCCTAATAGACGCGTTTTAAAAACCTTGAGGGAAAGCCCGGAAGGGAAAACCTAAAAAGGACAATATCTGCTGGCGGTGGATTCGGGCTTGTTATAAATGATATAAGAGCCAGACACCGGGCGATGTGTCAACGAGGAGGTTGAGCCACGAAGGGGGGTGGACACAAGGTGGTGTGCCAGTGAGGACGCTGAGCCCTGAAGGGGGGTGGATTGTGAGATCCCACCTCGGTTGGGGAGGAGAACAAAACATTCTTTATAAGGGTGTGGAAACTTCTCCCTAGCAGACACGTTTTAAAAACCTTGAGGGGAAGTCTAGAAGGGAAAGTCCAAAGAGGACAATATCTGCTAGCTGGACTTAGACTCTTATATTTCTATTATCTACTTGAGATCATGATGCCATTTCATTAAATGCAGGGTTGTAGAGCTTTACTGTTGGATAAAGATCGGAACCCGAAGGTATATGTCGGGTGTAGACATCTACATCTTCGAAATCTCTTGCTTGTTATTCGGGATTCTCAAATGTACATTCTTAAAAGTTTATCCAACCTTTATCTGATCTCATCTTTTATATTCTTCTTTAGTGGGAACCCTCAAAGCTGGGGCTTGTCACTGATGCAATGGTTGAGAGGTACTTCTCTAGGATTGAAGACGAAGAGTGGGAAGACCTAAAGCTCCCTGCAAGACCCAACTTGCCTGCTTCAGCCACTTCAAAGCTCTAGTTACCATGAGTTTGCAACTTACTCAGTTAATATATATTAGTACTTTGCTAGTTGTTTATGTCTATACATGGTGGTGGGTAAGACTGTTGGAAATAAGCCTTCCTCAATGAGGAATCAATAATTGATTTAATGTATTCTAAAATCAATGAACATACGTAAAATAATGTGTTCATAATAAAATTTCAATTTTACACCATGCTATAAATGATAACATTGTCCTTTGTTATTTCTTGTGAACCTATTTTCTATTGACGCAGGAGAGAAGGAAATATCACATCATCCATCCACAGATACCGCTTAAGCTGTGATAAAGGTATTCTAAATTTTCTACCGAACATTATGGATTGTACATTCCTTTCAACTTCAAACATCAATGAAATTTTATTACGTTGAAGGAATGACCTTAATTACATCGGTGTGATTATTAGAGATATTTGTGTAACCTCATTAAATAAGAAAAATAGTTAGACTTCCCTAATTTATGTGTAACCTCATTACCTAGTATCGAGAGATTTAGTTAGCTTTTTTGTAGTATTAAACACACATGTATCTCTCTGAAAAATTAAAAAGAAATGTGAGCAAGCTAATTTATGCTTACAATGTATCCTTTATATATATATATATATTGTAGAGCCATTCATGTTTCACCAATAATCCCTACAAGAACAGGAACCATCTTTGAAATGGTGGAAACGGTTTCGATCTCTCACCGTTATCTACCTTTTGTAAATCTTTATTGTAGTATGACAATCTGAGCAAGCTTAAGTATGAGATCTCACATCGGTTGGGGAGGAGAACGAAGTATTCTTTATAAGGGTATAAAAACCTCTCCCTAGCATACGCGTTTTAAATAACTTTGAGGGGAAGCCCGAAAGGAAAAGTCCAAATAGGACAATATTTGCTAGCAGTGAGCTTGACTATTACACTAAGGTAGGTTGTTCAGAATAACAATTCTTCCCCCTAACTTAGATTTCAATAAGCCATAAAGCTAGTTTTTCGCTATGTTAAGAAACCGATTCTTCCTTCTTATTATCATCAAAGTTGAGTAAACTTCTGAGATATCTGCAACTTAGCCTGCCAGCTTCAGCTTCTCTCTAACTTGTCTAAGCATCATAAATATTTCAGTTGTTTCTGGGTAAGAATAATCTCCCGCCAGAAATGAATGTGGGGCATTATCGATCGAACTGCAACCAGGTGTTTTCTGAACTCCTGTCTGATTCATCAATTCTCTAATCTTTTTTCACTTCATTCCACACTTTCCTTCTTGCATAAATGTTGGAGAGCAAAAGGTAAACTTCATCATTTGTTGGTTCAAGTTTTAGCAGGCATCTAGCCGCTTCCTCCCCAAGCTCAATATTGTTATATACTCGTTAAGCACCAACTATAGAGCCCCAGATTGCACCATTTGGTTCTAAAGACATTACTCGGATAAATTCCCGAGCTTCTACCATGTTAAAGATCATGTATTTGTTTAGATTGCTGTCAGTTAGTATCTGATTAAATCTCCTTATAGTTAGGAGTTGATTTAGTTTTAATGTTTAGATTGCTGTCGTTAGTATCTAATTAAATCTCCTGCTAGGGGTGTTTTTTAGTTTTGATTTGTTTATTGGTTTATTACCAGATTTTATGCAGATTTTTAGGTAGATTTTTTAATATCTTTTTATATTCTCTGGGTATATATAGAGCCTTTTTGCTCTTTTTAATATAATCTTCTCATCAACTATTCAATCACTCACTATTTCACTAACATGCCAAACAACCCTCTCTACATAAAAAATCTAGCATGAACCCATAATGCTCTCACTTAGGTTTGATTTGTTGAAGATGGCTGGGAGGAAGTCCCATGTGGCTAATTTAGGGAATGATCATAAGTTTATAAGTAAGGAAATACATCTCCATTGGTAATAAGGCCTTTTGGGGAAGCCCAAAGTAGAGTCATGAGAGCCTATGCTTAGAGTGACAATATCATACCATTGTGGAGATTCGTGATTCCTAACATGGTATCAGAGCCATGCCTTAAACTTAACCATGTCTATAGAATCCTCAAATATCGAACAAAGAATTTTGAGCCTCGAAGGTGTAGTCAAAAGTGACTAAAGTGTCGAACAAGGGGTGNAGTTTTGATTTGTTTATTGGTTTATTACCAGATTTTATGCAGATTTTTAGGTAGATTTTTTAATATCTTTTTATATTCTCTGGGTATATATAGAGCCTTTTTGCTCTTTTTAATATAATCTTCTCATCAACTATTCAATCACTCACTATTTCACTAACATGCCAAACAACCCTCTCTACATAAAAAATCTAGCATGAACCCATAATGCTCTCACTTAGGTTTGATTTGTTGAAGATGGCTGGGAGGAAGTCCCATGTGGCTAATTTAGGGAATGATCATAAGTTTATAAGTAAGGAAATACATCTCCATTGGTAATAAGGCCTTTTGGGGAAGCCCAAAGTAGAGTCATGAGAGCCTATGCTTAGAGTGACAATATCATACCATTGTGGAGATTCGTGATTCCTAACATGGTATCAGAGCCATGCCTTAAACTTAACCATGTCTATAGAATCCTCAAATATCGAACAAAGAATTTTGAGCCTCGAAGGTGTAGTCAAAAGTGACTAAAGTGTCGAACAAGGGGTGTACTTTGTTTGAGGGCTCCAAAGGAGTCGAGCCTCAATTAAGGGGAGGCTGTTCAAGAGTTTCATAAATCTCAGGGGAGGCTCTATAGTGTACTTTGTTCGAGGGAAGGATGGTTAGGAGGGAGTCCCACATTGGCTAATTTAGGGAATGATCCTGAGTTTATAAGTAAGGAAATACATCTCCATTGGTATGAGACCTTTTGGGGAAGCCTAAAGCAAAGCCATGAGAGCTTATGCTCAAAGTGGACAATATCATACCATTGTGGAGATCTGTGATTCCTAACATGATTCATTCCATAAAAAGCTCCCATTTCATGTAATTGCTCCATGCCCTCTTGAACTAACCCTGCGTAGCTGCAGGCACGCAGTACCTCTATCATCTGATCAAATCTCTTTTCGACATCCCATCAAAGATTCCTTTGGCAACATCTATTTCTCCACACTTCGACTACATGTACAGCTAGAATCGTGACTTCATTCGGACGCATGTTCGAGCCACCAACTATTTCCCTGATTAACTGCATCGCCTCTATGATTGTATTGTTCTCGACATAACCAAAAATAAAAGCCGACAAGCATACACGTCTCTTTCAGACAAACGATCAAATCCTTGCTTTGCAGTGTTTAGCTCCCCACATTTAGCATACATTAGGATCACGAACTTCCATAATGGTATGATATTGTCCACTTTTAACATAAGCTCTCATGACTTTACTTTTGGTTTTCCCAAAAGACCTTATTCCAGTGGAGATGTATTCTTTCCTTATAAACTCAAGATCAACTCCTTAATTAGCCGATGTGGGGCTCCCCACCAACAATCCTCCTCTCGAACAAAGTATACCATAGAGTCTCCCCCGAGGCCTATGAAGCCCTCAAACAACCTCCCATTAATTGAAGCTTGACTCCTTTGGAGACCTCAAACAAAGTATACCCTTTGTTCGACACTTGAGTCACTTTTGACNGGGTATATATAGAGCCTTTTTGCTCTTTTTAATATAATCTTCTCATCAACTATTCAATCACTCACTATTTCACTAACATGCCAAACAACCCTCTCTACATAAAAAATCTAGCATGAACCCATAATGCTCTCACTTAGGTTTGATTTGTTGAAGATGGCTGGGAGGAAGTCCCATGTGGCTAATTTAGGGAATGATCATAAGTTTATAAGTAAGGAAATACATCTCCATTGGTAATAAGGCCTTTTGGGGAAGCCCAAAGTAGAGTCATGAGAGCCTATGCTTAGAGTGACAATATCATACCATTGTGGAGANAGGCATAACTTTGATAACATGTTAGGAATCACGAACCTTCACAATAGTATGATATTGTCCACTTTGAGCATAAGCTCTCGTGGTTGTACTTTGGGTTTTCTCAAAAGGTCTTATTCCAATGGAGATTTATTCTTTATTTATAAACTCATAATCAACCCCTTAATTAGCCGATGTGGGACTCCCCACCAACAATCCTCACAAGCACTTCCCAAACACATCTCAAATTCTCGTACCCATCTCCGGAATTCGAGAATGCAGCTTCATTCCAACGCCCAAACCTTTCAATTGAGCACAAGCCAATACGAGTGTTGTCATGGTCATTTCATCGGGGTAAACCCATCTTCCTCCATTTTTCTCATACAGAAGAAATGCCTCATTAGGACAATGGTTTTTCGTACAACCACTAATCATCGAAATCCAAACCACATCGTTTCTATGACCCATTTTCTCAAACAAAACCTTCGTAGAGTTCAAATCCCCACAGGCAGCATACATATTTAACGATGTGGTTTCTATGTACAGGTCGGAATCAAGCATATACGGCACTTTGAGTTCCAATGAAAGGGCACAAACCTTCAGCAGTGAAGGAAATGTGAATCTATCAGCAACATTTCTCGACCATTGAAGCATCTCAAAGTAACTTTGGAGTGCCCTTCTCGGCATGTTGGAATTCACAAGGCCTCTCAACATGGTGTTCCAAGTAAGTATATCTAACTCTTGAGTGTGTGAAAAAAACTGAATAAGCATAGGCAAGACCAGGCGAGCCCATTGAGAGGACATGGGCAATGAGCTTGTTAAGTGATGGCTTATGGTGGATGAAGCCACTAACTACCATTTGTGCATGAAATTGCTGAAATTTTGTTGATTGTGTCCATTTTGTTGCTTCTTTCTAAGATGGGAAAGATAGGGTATGGT

mRNA sequence

CAGCCATAATCCTCGCTTGGAAGAATGGCGGCAAACTGAATCGTTAGCTATTTAGCTTCCACACTCATCTCTTTCCTATTTCATTTTGTTGAACCAGAGGTAGCATCTGCGAGCAATGGCGTCCTGGAATTCTACCGGAATTGATGAAGATCAGCAGGTTTTAGTGGAAGAGAAGCGGTTTGCGAGGATATTGACTTTAAACAGACCTAAGCAGTTAAATGCTATCTCATTCACAATGGTTTCGCGGCTATTGGAACTCTTTTCTTCCTATGAGACAGATCCCAGTGTGAAACTTGTGGTTGTCAAGGGTAAAGGAAGAGCATTTTGTGCTGGGGGTGATGTATCGGCTGCGGCTCATGCCATTAAAGAGAGTGAATGGAGGAAAGGTGCTCACTTCTTCCAGAAACAATACACCTTAAATTATTTGTTGGCAACTTTCAGTAAGCCACAGGTTTCTCTTCTGAATGGTATTGTCATGGGAGGAGGAGCCGGTGTTTCAATACATGGTAGATTCCGTGTTGCAACAGAGAAATCGATTTTTGCAATGCCCGAAACTTCTTTAGGACACTTCCCAGATGTTGGTGCTTCTTATTACTTGCCAAGACTCCGGGGATTTTTTGGCGAATATGTTGGCCTAACAGGTGCCAGGTTGGATGGTGCTGAAATGCTTGCATGTGGCCTTGCTACTCACTTTGTCCCTTCAGAGAGATTAACGCAATTAGAGGAAGCACTATGCAGAGCAGATACAAGTCAACCAAGCATCATCTCTGCAATTATTGATAAATTCTCCCAGACACCAATTCTTCACGAGCAAAGTGCTTATAACCGGTTGGATGTTATTGATAAGTGCTTCTCCCGAAGAACAGTAGAAGACATTTTGGCAGCTCTTGAAGAGGAGGCCCTGAAAAAGAAAGATAATTGGATCAATTCAACAATTCGAGACTTGAAGAAGGCATCACCAACAAGTCTTAAAATCTCTTTGAGATCGATAAGAGAAGGCAGACTTCAAGATGCTGGTCAGTGTCTCATTCGTGAGTATAGAATGGCCTGTCATGTCATGCGAGGGGAATGGGAACCCTCAAAGCTGGGGCTTGTCACTGATGCAATGGTTGAGAGGTACTTCTCTAGGATTGAAGACGAAGAGTGGGAAGACCTAAAGCTCCCTGCAAGACCCAACTTGCCTGCTTCAGCCACTTCAAAGCTCTAGTTACCATGAGTTTGCAACTTACTCAGAGAGAAGGAAATATCACATCATCCATCCACAGATACCGCTTAAGCTGTGATAAAGGTCGGAATCAAGCATATACGGCACTTTGAGTTCCAATGAAAGGGCACAAACCTTCAGCAGTGAAGGAAATGTGAATCTATCAGCAACATTTCTCGACCATTGAAGCATCTCAAAGTAACTTTGGAGTGCCCTTCTCGGCATGTTGGAATTCACAAGGCCTCTCAACATGGTGTTCCAAGTAAGTATATCTAACTCTTGAGTGTGTGAAAAAAACTGAATAAGCATAGGCAAGACCAGGCGAGCCCATTGAGAGGACATGGGCAATGAGCTTGTTAAGTGATGGCTTATGGTGGATGAAGCCACTAACTACCATTTGTGCATGAAATTGCTGAAATTTTGTTGATTGTGTCCATTTTGTTGCTTCTTTCTAAGATGGGAAAGATAGGGTATGGT

Coding sequence (CDS)

ATGGCGTCCTGGAATTCTACCGGAATTGATGAAGATCAGCAGGTTTTAGTGGAAGAGAAGCGGTTTGCGAGGATATTGACTTTAAACAGACCTAAGCAGTTAAATGCTATCTCATTCACAATGGTTTCGCGGCTATTGGAACTCTTTTCTTCCTATGAGACAGATCCCAGTGTGAAACTTGTGGTTGTCAAGGGTAAAGGAAGAGCATTTTGTGCTGGGGGTGATGTATCGGCTGCGGCTCATGCCATTAAAGAGAGTGAATGGAGGAAAGGTGCTCACTTCTTCCAGAAACAATACACCTTAAATTATTTGTTGGCAACTTTCAGTAAGCCACAGGTTTCTCTTCTGAATGGTATTGTCATGGGAGGAGGAGCCGGTGTTTCAATACATGGTAGATTCCGTGTTGCAACAGAGAAATCGATTTTTGCAATGCCCGAAACTTCTTTAGGACACTTCCCAGATGTTGGTGCTTCTTATTACTTGCCAAGACTCCGGGGATTTTTTGGCGAATATGTTGGCCTAACAGGTGCCAGGTTGGATGGTGCTGAAATGCTTGCATGTGGCCTTGCTACTCACTTTGTCCCTTCAGAGAGATTAACGCAATTAGAGGAAGCACTATGCAGAGCAGATACAAGTCAACCAAGCATCATCTCTGCAATTATTGATAAATTCTCCCAGACACCAATTCTTCACGAGCAAAGTGCTTATAACCGGTTGGATGTTATTGATAAGTGCTTCTCCCGAAGAACAGTAGAAGACATTTTGGCAGCTCTTGAAGAGGAGGCCCTGAAAAAGAAAGATAATTGGATCAATTCAACAATTCGAGACTTGAAGAAGGCATCACCAACAAGTCTTAAAATCTCTTTGAGATCGATAAGAGAAGGCAGACTTCAAGATGCTGGTCAGTGTCTCATTCGTGAGTATAGAATGGCCTGTCATGTCATGCGAGGGGAATGGGAACCCTCAAAGCTGGGGCTTGTCACTGATGCAATGGTTGAGAGGTACTTCTCTAGGATTGAAGACGAAGAGTGGGAAGACCTAAAGCTCCCTGCAAGACCCAACTTGCCTGCTTCAGCCACTTCAAAGCTCTAG

Protein sequence

MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACHVMRGEWEPSKLGLVTDAMVERYFSRIEDEEWEDLKLPARPNLPASATSKL

Homology

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy Swiss-Prot
Match: Q9LKJ1 (3-hydroxyisobutyryl-CoA hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=CHY1 PE=1 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 1.7e-135
Identity = 243/371 (65.50%), Postives = 290/371 (78.17%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILTLNRPKQLNA+SF M+SRLL+LF ++E DPSVKLV++KG GRAFCAG
Sbjct: 10  QVLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAG 69

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GDV+A    I +  WR GA++F  +Y LNY++AT+SK QVS+LNGIVMGGGAGVS+HGRF
Sbjct: 70  GDVAAVVRDINQGNWRLGANYFSSEYMLNYVMATYSKAQVSILNGIVMGGGAGVSVHGRF 129

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPET+LG FPDVGASY+L RL GFFGEYVGLTGARLDGAEMLACGLATHF
Sbjct: 130 RIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHF 189

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS RLT LE  LCR +++ P+  S I+D ++Q P L +QSAY RLDVID+CFSRRTVE+
Sbjct: 190 VPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYRRLDVIDRCFSRRTVEE 249

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I++ALE EA ++ D WI++TI+ LKK SP SLKISLRSIREGRLQ  GQCLIREYRM CH
Sbjct: 250 IISALEREATQEADGWISATIQALKKGSPASLKISLRSIREGRLQGVGQCLIREYRMVCH 309

Query: 314 VMRGE---------------------WEPSKLGLVTDAMVERYFSRIEDEEWEDLKLPAR 364
           VM+GE                     WEP +L  + D+MVE+YF R+E E  +DLKLP R
Sbjct: 310 VMKGEISKDFVEGCRAILVDKDKNPKWEPRRLEDMKDSMVEQYFERVERE--DDLKLPPR 369

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy Swiss-Prot
Match: Q6NMB0 (Probable 3-hydroxyisobutyryl-CoA hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g30660 PE=2 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 1.4e-132
Identity = 236/366 (64.48%), Postives = 287/366 (78.42%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILTLNRPKQLNA+ F M+SRLL+LF +YE DPSVKLV++KG+GRAFCAG
Sbjct: 6   QVLVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAG 65

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GDV      + + +WR GA FF+ QYTLNY++AT+SKPQVS+LNGIVMG GAGVSIHGRF
Sbjct: 66  GDVPPVVQNMVQGKWRLGADFFRDQYTLNYVMATYSKPQVSILNGIVMGAGAGVSIHGRF 125

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPETSLG FPDVGASY+L RL GFFGEYVGLTGARLDGAE+LACGLATHF
Sbjct: 126 RIATENTVFAMPETSLGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAELLACGLATHF 185

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS RLT LE  LC+  +S PS +S I+D ++Q P L ++SAY+RLDVID+CFS+RT+E+
Sbjct: 186 VPSTRLTALETDLCKVGSSDPSFVSTILDAYTQHPHLKQKSAYHRLDVIDRCFSKRTMEE 245

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I++ALE E  ++ D+W  +TIR LKK+SP+SLKISLRSIREGRLQ  G CL REYRM CH
Sbjct: 246 IISALERETTQELDDWSLTTIRALKKSSPSSLKISLRSIREGRLQGVGHCLTREYRMVCH 305

Query: 314 VMRGE---------------------WEPSKLGLVTDAMVERYFSRIEDEE-WEDLKLPA 358
           VM+G+                     WEP +L  + D+MV+++F R+E+EE WEDLKL  
Sbjct: 306 VMKGDLSKDLVEGCRAILIDKDRNPKWEPRRLEDMKDSMVDQFFERVEEEERWEDLKLSP 365

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy Swiss-Prot
Match: Q1PEY5 (Probable 3-hydroxyisobutyryl-CoA hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=At2g30650 PE=2 SV=1)

HSP 1 Score: 458.0 bits (1177), Expect = 1.0e-127
Identity = 232/368 (63.04%), Postives = 285/368 (77.45%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILT NRPKQLNA+SF MVSRLL+LF +YE DPSVKLVV+KG+GRAF AG
Sbjct: 6   QVLVEEKSSVRILTFNRPKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAG 65

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GD+      I + +  +GAH+F+  YTLNY+L+T+ KPQVS+LNGIVMGGGAG+S +GRF
Sbjct: 66  GDIPPIVRDILQGKLIRGAHYFKVGYTLNYVLSTYRKPQVSILNGIVMGGGAGLSTNGRF 125

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPET+LG FPDVGASY+L RL GFFGEYVGLTGARLDGAEMLACGLATHF
Sbjct: 126 RIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHF 185

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS  LT LE  L +  +S  + IS I+D +++ P L++ S+Y+RLDVID+CFS+RTVE+
Sbjct: 186 VPSISLTALEAELYKVGSSNQTFISTILDAYAEYPHLNQHSSYHRLDVIDRCFSKRTVEE 245

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I +ALE E  +K ++W+ +TI+ L+KASP+ LKISLRSIREGRLQ  GQCLIREYRM CH
Sbjct: 246 IFSALEREVTQKPNDWLLATIQALEKASPSCLKISLRSIREGRLQGVGQCLIREYRMVCH 305

Query: 314 VMRGE---------------------WEPSKLGLVTDAMVERYFSRIEDEE-WEDLKLPA 360
           VM+G+                     W+P +L  VTD+MV++YF R+EDEE WEDLK P 
Sbjct: 306 VMKGDISKDFVEGCRAVLIDKDRNPKWQPRRLEDVTDSMVDQYFERVEDEEGWEDLKFPP 365

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy Swiss-Prot
Match: Q5XF59 (3-hydroxyisobutyryl-CoA hydrolase-like protein 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g60510 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 5.2e-84
Identity = 168/373 (45.04%), Postives = 231/373 (61.93%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           + S   T  D D QVLVE    +R   LNRP  LNA++  M  RL +L+ ++E DP++  
Sbjct: 25  LCSLKLTSEDLDYQVLVEGSGCSRTAILNRPPALNALTTHMGYRLQKLYKNWEEDPNIGF 84

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           V++KG GRAFCAGGD+ +  H            FF   Y+  YLL T+ KP V++LNG+ 
Sbjct: 85  VMMKGSGRAFCAGGDIVSLYHLRTRGSPDAIREFFSSLYSFIYLLGTYLKPHVAILNGVT 144

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGG GVSI G FRVAT+++IFA PET +G  PD GAS+ L  L G  GEY+GLTG +L 
Sbjct: 145 MGGGTGVSIPGTFRVATDRTIFATPETIIGFHPDAGASFNLSHLPGRLGEYLGLTGLKLS 204

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATH++ SE +  +EE L +  T  PS++ + ++K ++     +     R+D
Sbjct: 205 GAEMLACGLATHYIRSEEVPVMEEQLKKLLTDDPSVVESCLEKCAEVAHPEKTGVIRRID 264

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           +++KCFS  TVE+I+ +LE EA ++KD W  +T+R LK++SP SLK++LRSIREGRLQ  
Sbjct: 265 LLEKCFSHDTVEEIIDSLEIEASRRKDTWCITTLRRLKESSPLSLKVALRSIREGRLQTL 324

Query: 301 GQCLIREYRMA------------CHVMRG---------EWEPSKLGLVTDAMVERYFSRI 353
            QCLIREYRM+            C  +R          +W+P  L  V++ MV+ YF  +
Sbjct: 325 DQCLIREYRMSLQGLIGPMSGNFCEGVRARLIDKDEAPKWDPPSLEKVSEDMVDDYFCAL 384

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy Swiss-Prot
Match: Q8RXN4 (3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g31810 PE=1 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 3.1e-76
Identity = 149/380 (39.21%), Postives = 226/380 (59.47%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           + +++++  D + QVLVE K  +R   LN P  LNA+S  MV RL  L+ S+E +P++  
Sbjct: 29  LPNYSASDADFEDQVLVEGKAKSRAAILNNPSSLNALSAPMVGRLKRLYESWEENPAISF 88

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           V++KG G+ FC+G DV +  H+I E    +   FF+  Y   YL  T+ KP +++++G+ 
Sbjct: 89  VLMKGSGKTFCSGADVLSLYHSINEGNTEESKLFFENLYKFVYLQGTYLKPNIAIMDGVT 148

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MG G G+S+ G FRVAT+K++ A PE  +G  PD GASYYL RL G+ GEY+ LTG +L+
Sbjct: 149 MGCGGGISLPGMFRVATDKTVLAHPEVQIGFHPDAGASYYLSRLPGYLGEYLALTGQKLN 208

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           G EM+ACGLATH+  + RL  +EE + +  T  P++I   + ++         S  ++++
Sbjct: 209 GVEMIACGLATHYCLNARLPLIEERIGKLLTDDPAVIEDSLAQYGDLVYPDSSSVLHKIE 268

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           +IDK F   TVE+I+ A+E EA    + W   T++ +K+ASP SLKI+L+SIREGR Q  
Sbjct: 269 LIDKYFGLDTVEEIIEAMENEAANSCNEWCKKTLKQIKEASPLSLKITLQSIREGRFQTL 328

Query: 301 GQCLIREYRMA------------CHVMRG---------EWEPSKLGLVTDAMVERYF--- 357
            QCL  EYR++            C  +R          +W+P +L  V+  MV+ YF   
Sbjct: 329 DQCLTHEYRISICGVSKVVSGDFCEGIRARLVDKDFAPKWDPPRLEDVSKDMVDCYFTPA 388

[Close]

BLAST of Cp4.1LG08g02160 vs. NCBI nr
Match: XP_023539070.1 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708 bits (1827), Expect = 1.34e-256
Identity = 363/384 (94.53%), Postives = 363/384 (94.53%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL
Sbjct: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD
Sbjct: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKLGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKLPARPNLPASATSKL
Sbjct: 361 EDEEWEDLKLPARPNLPASATSKL 384

[Close]

BLAST of Cp4.1LG08g02160 vs. NCBI nr
Match: XP_023539071.1 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 701 bits (1810), Expect = 5.02e-254
Identity = 362/384 (94.27%), Postives = 362/384 (94.27%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQ VLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL
Sbjct: 1   MASWNSTGIDEDQ-VLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD
Sbjct: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKLGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKLPARPNLPASATSKL
Sbjct: 361 EDEEWEDLKLPARPNLPASATSKL 383

[Close]

BLAST of Cp4.1LG08g02160 vs. NCBI nr
Match: XP_022974145.1 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita maxima] >XP_022974146.1 3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 689 bits (1778), Expect = 3.92e-249
Identity = 355/384 (92.45%), Postives = 357/384 (92.97%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDP VKL
Sbjct: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPCVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSA AHAIKESEWRKGAHFF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAVAHAIKESEWRKGAHFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
            AEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD
Sbjct: 181 AAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIR+GRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIRKGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKLGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKLPAR NL ASATSKL
Sbjct: 361 EDEEWEDLKLPARLNLSASATSKL 384

[Close]

BLAST of Cp4.1LG08g02160 vs. NCBI nr
Match: XP_022959992.1 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita moschata] >XP_022960070.1 3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 688 bits (1776), Expect = 7.91e-249
Identity = 354/384 (92.19%), Postives = 360/384 (93.75%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQQVLVEE+RFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL
Sbjct: 1   MASWNSTGIDEDQQVLVEERRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGA+FF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAYFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFS+TPILHEQSAYNRLD
Sbjct: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSRTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKD+WI+STIRDLKKASPTSLKISLRSIREGRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDDWIDSTIRDLKKASPTSLKISLRSIREGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKL LVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLELVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKL ARPNLPASATSKL
Sbjct: 361 EDEEWEDLKLTARPNLPASATSKL 384

[Close]

BLAST of Cp4.1LG08g02160 vs. NCBI nr
Match: XP_022974147.1 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 682 bits (1761), Expect = 1.47e-246
Identity = 354/384 (92.19%), Postives = 356/384 (92.71%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQ VLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDP VKL
Sbjct: 1   MASWNSTGIDEDQ-VLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPCVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSA AHAIKESEWRKGAHFF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAVAHAIKESEWRKGAHFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
            AEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD
Sbjct: 181 AAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIR+GRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIRKGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKLGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKLPAR NL ASATSKL
Sbjct: 361 EDEEWEDLKLPARLNLSASATSKL 383

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy TrEMBL
Match: A0A6J1IAJ5 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472738 PE=3 SV=1)

HSP 1 Score: 689 bits (1778), Expect = 1.90e-249
Identity = 355/384 (92.45%), Postives = 357/384 (92.97%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDP VKL
Sbjct: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPCVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSA AHAIKESEWRKGAHFF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAVAHAIKESEWRKGAHFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
            AEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD
Sbjct: 181 AAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIR+GRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIRKGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKLGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKLPAR NL ASATSKL
Sbjct: 361 EDEEWEDLKLPARLNLSASATSKL 384

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy TrEMBL
Match: A0A6J1H642 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111460854 PE=3 SV=1)

HSP 1 Score: 688 bits (1776), Expect = 3.83e-249
Identity = 354/384 (92.19%), Postives = 360/384 (93.75%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQQVLVEE+RFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL
Sbjct: 1   MASWNSTGIDEDQQVLVEERRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGA+FF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAYFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFS+TPILHEQSAYNRLD
Sbjct: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSRTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKD+WI+STIRDLKKASPTSLKISLRSIREGRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDDWIDSTIRDLKKASPTSLKISLRSIREGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKL LVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLELVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKL ARPNLPASATSKL
Sbjct: 361 EDEEWEDLKLTARPNLPASATSKL 384

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy TrEMBL
Match: A0A6J1I9H2 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472738 PE=3 SV=1)

HSP 1 Score: 682 bits (1761), Expect = 7.13e-247
Identity = 354/384 (92.19%), Postives = 356/384 (92.71%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQ VLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDP VKL
Sbjct: 1   MASWNSTGIDEDQ-VLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPCVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSA AHAIKESEWRKGAHFF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAVAHAIKESEWRKGAHFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
            AEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD
Sbjct: 181 AAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIR+GRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIRKGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKLGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKLPAR NL ASATSKL
Sbjct: 361 EDEEWEDLKLPARLNLSASATSKL 383

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy TrEMBL
Match: A0A6J1H6Y1 (3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111460854 PE=3 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 1.44e-246
Identity = 353/384 (91.93%), Postives = 359/384 (93.49%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MASWNSTGIDEDQ VLVEE+RFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL
Sbjct: 1   MASWNSTGIDEDQ-VLVEERRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGA+FF+KQYTLNYLLAT SKPQVSLLNGIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAYFFRKQYTLNYLLATSSKPQVSLLNGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFS+TPILHEQSAYNRLD
Sbjct: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSRTPILHEQSAYNRLD 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALEEEALKKKD+WI+STIRDLKKASPTSLKISLRSIREGRLQDA
Sbjct: 241 VIDKCFSRRTVEDILAALEEEALKKKDDWIDSTIRDLKKASPTSLKISLRSIREGRLQDA 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRMACHVMRGE                     WEPSKL LVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMACHVMRGEVSEDFIEGCRALLLDKDRNPKWEPSKLELVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDLKL ARPNLPASATSKL
Sbjct: 361 EDEEWEDLKLTARPNLPASATSKL 383

[Close]

BLAST of Cp4.1LG08g02160 vs. ExPASy TrEMBL
Match: A0A1S3AVW3 (3-hydroxyisobutyryl-CoA hydrolase 1-like OS=Cucumis melo OX=3656 GN=LOC103483400 PE=3 SV=1)

HSP 1 Score: 604 bits (1557), Expect = 8.48e-216
Identity = 313/384 (81.51%), Postives = 333/384 (86.72%), Query Frame = 0


Query: 1   MASWNSTGIDEDQQVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKL 60
           MAS++ST  DEDQ VLVEEKRF R+LTLNR KQLNA+S TMVSRLLELF +YE DPSVKL
Sbjct: 1   MASFDSTRTDEDQ-VLVEEKRFVRLLTLNRTKQLNALSLTMVSRLLELFVAYEADPSVKL 60

Query: 61  VVVKGKGRAFCAGGDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIV 120
           VVVKGKGRAFCAGGDVSA  H IK  +W+KGA+FFQK++T NYLLATFSKPQVSLL+GIV
Sbjct: 61  VVVKGKGRAFCAGGDVSAVVHDIKRGDWKKGAYFFQKEFTFNYLLATFSKPQVSLLDGIV 120

Query: 121 MGGGAGVSIHGRFRVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLD 180
           MGGGAGVSIHGRFRVATEKS+FAMPET+LG FPDVGASYYL RL GFFGEYVGLTGARLD
Sbjct: 121 MGGGAGVSIHGRFRVATEKSVFAMPETALGLFPDVGASYYLSRLPGFFGEYVGLTGARLD 180

Query: 181 GAEMLACGLATHFVPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLD 240
           GAEMLACGLATHFV SERLT LEEALCRADTS+PSIIS+IIDKFSQ PIL EQS Y+RL 
Sbjct: 181 GAEMLACGLATHFVTSERLTLLEEALCRADTSEPSIISSIIDKFSQRPILKEQSVYHRLH 240

Query: 241 VIDKCFSRRTVEDILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDA 300
           VIDKCFSRRTVEDILAALE+EALKKKD+WI STI+DLKKASPTSLKISLRSIREGRLQ  
Sbjct: 241 VIDKCFSRRTVEDILAALEDEALKKKDDWITSTIQDLKKASPTSLKISLRSIREGRLQGV 300

Query: 301 GQCLIREYRMACHVMRGE---------------------WEPSKLGLVTDAMVERYFSRI 360
           GQCLIREYRM CHVMRGE                     WEPS LGLVTDAMVERYFSRI
Sbjct: 301 GQCLIREYRMVCHVMRGEVSKDFVEGCRAILLDKDRNPKWEPSSLGLVTDAMVERYFSRI 360

Query: 361 EDEEWEDLKLPARPNLPASATSKL 363
           EDEEWEDL+LPAR NLPASA SKL
Sbjct: 361 EDEEWEDLQLPARANLPASAISKL 383

[Close]

BLAST of Cp4.1LG08g02160 vs. TAIR 10
Match: AT5G65940.1 (beta-hydroxyisobutyryl-CoA hydrolase 1 )

HSP 1 Score: 483.8 bits (1244), Expect = 1.2e-136
Identity = 243/371 (65.50%), Postives = 290/371 (78.17%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILTLNRPKQLNA+SF M+SRLL+LF ++E DPSVKLV++KG GRAFCAG
Sbjct: 10  QVLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAG 69

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GDV+A    I +  WR GA++F  +Y LNY++AT+SK QVS+LNGIVMGGGAGVS+HGRF
Sbjct: 70  GDVAAVVRDINQGNWRLGANYFSSEYMLNYVMATYSKAQVSILNGIVMGGGAGVSVHGRF 129

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPET+LG FPDVGASY+L RL GFFGEYVGLTGARLDGAEMLACGLATHF
Sbjct: 130 RIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHF 189

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS RLT LE  LCR +++ P+  S I+D ++Q P L +QSAY RLDVID+CFSRRTVE+
Sbjct: 190 VPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYRRLDVIDRCFSRRTVEE 249

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I++ALE EA ++ D WI++TI+ LKK SP SLKISLRSIREGRLQ  GQCLIREYRM CH
Sbjct: 250 IISALEREATQEADGWISATIQALKKGSPASLKISLRSIREGRLQGVGQCLIREYRMVCH 309

Query: 314 VMRGE---------------------WEPSKLGLVTDAMVERYFSRIEDEEWEDLKLPAR 364
           VM+GE                     WEP +L  + D+MVE+YF R+E E  +DLKLP R
Sbjct: 310 VMKGEISKDFVEGCRAILVDKDKNPKWEPRRLEDMKDSMVEQYFERVERE--DDLKLPPR 369

[Close]

BLAST of Cp4.1LG08g02160 vs. TAIR 10
Match: AT2G30660.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 474.2 bits (1219), Expect = 9.6e-134
Identity = 236/366 (64.48%), Postives = 287/366 (78.42%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILTLNRPKQLNA+ F M+SRLL+LF +YE DPSVKLV++KG+GRAFCAG
Sbjct: 6   QVLVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAG 65

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GDV      + + +WR GA FF+ QYTLNY++AT+SKPQVS+LNGIVMG GAGVSIHGRF
Sbjct: 66  GDVPPVVQNMVQGKWRLGADFFRDQYTLNYVMATYSKPQVSILNGIVMGAGAGVSIHGRF 125

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPETSLG FPDVGASY+L RL GFFGEYVGLTGARLDGAE+LACGLATHF
Sbjct: 126 RIATENTVFAMPETSLGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAELLACGLATHF 185

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS RLT LE  LC+  +S PS +S I+D ++Q P L ++SAY+RLDVID+CFS+RT+E+
Sbjct: 186 VPSTRLTALETDLCKVGSSDPSFVSTILDAYTQHPHLKQKSAYHRLDVIDRCFSKRTMEE 245

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I++ALE E  ++ D+W  +TIR LKK+SP+SLKISLRSIREGRLQ  G CL REYRM CH
Sbjct: 246 IISALERETTQELDDWSLTTIRALKKSSPSSLKISLRSIREGRLQGVGHCLTREYRMVCH 305

Query: 314 VMRGE---------------------WEPSKLGLVTDAMVERYFSRIEDEE-WEDLKLPA 358
           VM+G+                     WEP +L  + D+MV+++F R+E+EE WEDLKL  
Sbjct: 306 VMKGDLSKDLVEGCRAILIDKDRNPKWEPRRLEDMKDSMVDQFFERVEEEERWEDLKLSP 365

[Close]

BLAST of Cp4.1LG08g02160 vs. TAIR 10
Match: AT2G30650.1 (ATP-dependent caseinolytic (Clp) protease/crotonase family protein )

HSP 1 Score: 458.0 bits (1177), Expect = 7.1e-129
Identity = 232/368 (63.04%), Postives = 285/368 (77.45%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILT NRPKQLNA+SF MVSRLL+LF +YE DPSVKLVV+KG+GRAF AG
Sbjct: 6   QVLVEEKSSVRILTFNRPKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAG 65

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GD+      I + +  +GAH+F+  YTLNY+L+T+ KPQVS+LNGIVMGGGAG+S +GRF
Sbjct: 66  GDIPPIVRDILQGKLIRGAHYFKVGYTLNYVLSTYRKPQVSILNGIVMGGGAGLSTNGRF 125

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPET+LG FPDVGASY+L RL GFFGEYVGLTGARLDGAEMLACGLATHF
Sbjct: 126 RIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHF 185

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS  LT LE  L +  +S  + IS I+D +++ P L++ S+Y+RLDVID+CFS+RTVE+
Sbjct: 186 VPSISLTALEAELYKVGSSNQTFISTILDAYAEYPHLNQHSSYHRLDVIDRCFSKRTVEE 245

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I +ALE E  +K ++W+ +TI+ L+KASP+ LKISLRSIREGRLQ  GQCLIREYRM CH
Sbjct: 246 IFSALEREVTQKPNDWLLATIQALEKASPSCLKISLRSIREGRLQGVGQCLIREYRMVCH 305

Query: 314 VMRGE---------------------WEPSKLGLVTDAMVERYFSRIEDEE-WEDLKLPA 360
           VM+G+                     W+P +L  VTD+MV++YF R+EDEE WEDLK P 
Sbjct: 306 VMKGDISKDFVEGCRAVLIDKDRNPKWQPRRLEDVTDSMVDQYFERVEDEEGWEDLKFPP 365

[Close]

BLAST of Cp4.1LG08g02160 vs. TAIR 10
Match: AT5G65940.2 (beta-hydroxyisobutyryl-CoA hydrolase 1 )

HSP 1 Score: 449.5 bits (1155), Expect = 2.5e-126
Identity = 218/305 (71.48%), Postives = 258/305 (84.59%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILTLNRPKQLNA+SF M+SRLL+LF ++E DPSVKLV++KG GRAFCAG
Sbjct: 10  QVLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAG 69

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GDV+A    I +  WR GA++F  +Y LNY++AT+SK QVS+LNGIVMGGGAGVS+HGRF
Sbjct: 70  GDVAAVVRDINQGNWRLGANYFSSEYMLNYVMATYSKAQVSILNGIVMGGGAGVSVHGRF 129

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPET+LG FPDVGASY+L RL GFFGEYVGLTGARLDGAEMLACGLATHF
Sbjct: 130 RIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHF 189

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS RLT LE  LCR +++ P+  S I+D ++Q P L +QSAY RLDVID+CFSRRTVE+
Sbjct: 190 VPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYRRLDVIDRCFSRRTVEE 249

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I++ALE EA ++ D WI++TI+ LKK SP SLKISLRSIREGRLQ  GQCLIREYRM CH
Sbjct: 250 IISALEREATQEADGWISATIQALKKGSPASLKISLRSIREGRLQGVGQCLIREYRMVCH 309

Query: 314 VMRGE 319
           VM+GE
Sbjct: 310 VMKGE 314

[Close]

BLAST of Cp4.1LG08g02160 vs. TAIR 10
Match: AT5G65940.3 (beta-hydroxyisobutyryl-CoA hydrolase 1 )

HSP 1 Score: 449.5 bits (1155), Expect = 2.5e-126
Identity = 218/305 (71.48%), Postives = 258/305 (84.59%), Query Frame = 0


Query: 14  QVLVEEKRFARILTLNRPKQLNAISFTMVSRLLELFSSYETDPSVKLVVVKGKGRAFCAG 73
           QVLVEEK   RILTLNRPKQLNA+SF M+SRLL+LF ++E DPSVKLV++KG GRAFCAG
Sbjct: 10  QVLVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAG 69

Query: 74  GDVSAAAHAIKESEWRKGAHFFQKQYTLNYLLATFSKPQVSLLNGIVMGGGAGVSIHGRF 133
           GDV+A    I +  WR GA++F  +Y LNY++AT+SK QVS+LNGIVMGGGAGVS+HGRF
Sbjct: 70  GDVAAVVRDINQGNWRLGANYFSSEYMLNYVMATYSKAQVSILNGIVMGGGAGVSVHGRF 129

Query: 134 RVATEKSIFAMPETSLGHFPDVGASYYLPRLRGFFGEYVGLTGARLDGAEMLACGLATHF 193
           R+ATE ++FAMPET+LG FPDVGASY+L RL GFFGEYVGLTGARLDGAEMLACGLATHF
Sbjct: 130 RIATENTVFAMPETALGLFPDVGASYFLSRLPGFFGEYVGLTGARLDGAEMLACGLATHF 189

Query: 194 VPSERLTQLEEALCRADTSQPSIISAIIDKFSQTPILHEQSAYNRLDVIDKCFSRRTVED 253
           VPS RLT LE  LCR +++ P+  S I+D ++Q P L +QSAY RLDVID+CFSRRTVE+
Sbjct: 190 VPSTRLTALEADLCRINSNDPTFASTILDAYTQHPRLKQQSAYRRLDVIDRCFSRRTVEE 249

Query: 254 ILAALEEEALKKKDNWINSTIRDLKKASPTSLKISLRSIREGRLQDAGQCLIREYRMACH 313
           I++ALE EA ++ D WI++TI+ LKK SP SLKISLRSIREGRLQ  GQCLIREYRM CH
Sbjct: 250 IISALEREATQEADGWISATIQALKKGSPASLKISLRSIREGRLQGVGQCLIREYRMVCH 309

Query: 314 VMRGE 319
           VM+GE
Sbjct: 310 VMKGE 314

The following BLAST results are available for this feature:

BLAST of Cp4.1LG08g02160 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LKJ1	1.7e-135	65.50	3-hydroxyisobutyryl-CoA hydrolase 1 OS=Arabidopsis thaliana OX=3702 GN=CHY1 PE=1...	[more]
Q6NMB0	1.4e-132	64.48	Probable 3-hydroxyisobutyryl-CoA hydrolase 3 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q1PEY5	1.0e-127	63.04	Probable 3-hydroxyisobutyryl-CoA hydrolase 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q5XF59	5.2e-84	45.04	3-hydroxyisobutyryl-CoA hydrolase-like protein 1, mitochondrial OS=Arabidopsis t...	[more]
Q8RXN4	3.1e-76	39.21	3-hydroxyisobutyryl-CoA hydrolase-like protein 2, mitochondrial OS=Arabidopsis t...	[more]

BLAST of Cp4.1LG08g02160 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023539070.1	1.34e-256	94.53	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023539071.1	5.02e-254	94.27	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022974145.1	3.92e-249	92.45	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita maxima] >XP_02297...	[more]
XP_022959992.1	7.91e-249	92.19	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 [Cucurbita moschata] >XP_022...	[more]
XP_022974147.1	1.47e-246	92.19	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 [Cucurbita maxima]	[more]

BLAST of Cp4.1LG08g02160 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IAJ5	1.90e-249	92.45	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1H642	3.83e-249	92.19	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X1 OS=Cucurbita moschata OX=366...	[more]
A0A6J1I9H2	7.13e-247	92.19	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 OS=Cucurbita maxima OX=3661 ...	[more]
A0A6J1H6Y1	1.44e-246	91.93	3-hydroxyisobutyryl-CoA hydrolase 1-like isoform X2 OS=Cucurbita moschata OX=366...	[more]
A0A1S3AVW3	8.48e-216	81.51	3-hydroxyisobutyryl-CoA hydrolase 1-like OS=Cucumis melo OX=3656 GN=LOC103483400...	[more]

BLAST of Cp4.1LG08g02160 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65940.1	1.2e-136	65.50	beta-hydroxyisobutyryl-CoA hydrolase 1	[more]
AT2G30660.1	9.6e-134	64.48	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT2G30650.1	7.1e-129	63.04	ATP-dependent caseinolytic (Clp) protease/crotonase family protein	[more]
AT5G65940.2	2.5e-126	71.48	beta-hydroxyisobutyryl-CoA hydrolase 1	[more]
AT5G65940.3	2.5e-126	71.48	beta-hydroxyisobutyryl-CoA hydrolase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 248..268
None	No IPR available	GENE3D	3.90.226.10		coord: 9..349 e-value: 2.1E-116 score: 390.9
None	No IPR available	PANTHER	PTHR43176:SF20	BNAA02G34880D PROTEIN	coord: 318..363 coord: 12..318
None	No IPR available	CDD	cd06558	crotonase-like	coord: 15..208 e-value: 3.59438E-58 score: 185.457
IPR045004	Enoyl-CoA hydratase/isomerase domain	PFAM	PF16113	ECH_2	coord: 24..316 e-value: 6.0E-109 score: 364.6
IPR032259	Enoyl-CoA hydratase/isomerase, HIBYL-CoA-H type	PANTHER	PTHR43176	3-HYDROXYISOBUTYRYL-COA HYDROLASE-RELATED	coord: 318..363 coord: 12..318
IPR029045	ClpP/crotonase-like domain superfamily	SUPERFAMILY	52096	ClpP/crotonase	coord: 13..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG08g02160.1	Cp4.1LG08g02160.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006574	valine catabolic process
molecular_function	GO:0003860	3-hydroxyisobutyryl-CoA hydrolase activity