Cp4.1LG07g10900 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG07g10900
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptioncalcium uptake protein, mitochondrial-like
LocationCp4.1LG07: 10082881 .. 10086789 (+)
RNA-Seq ExpressionCp4.1LG07g10900
SyntenyCp4.1LG07g10900
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATAATTACAAAAGATGGGGTGCTTTTCGTATCCAACATTTTAACCTAATGGATCACGGTAAATTTAATCTTTTGTTCATATTTTGCGTTCGATCATTTCCAGATGATTACAGAAGACGAGTGTTTTTCAACTACGAAAAACGAATAAGAATCCAAAGCCCTCCCGAGAAGGTGTTCGAGTATTTTGCATCTATTCACACAGCGGACGGAGAAATTTTCATGACACCATCGGATCTAATGAGAGCGATTGTTCCTGTATTTCCTCCATCGGAGTCGAATCGAGTAAGAGAAGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTCTATTGTGTTCCTTCTAAATTCTTCATGCTTTTTGACACCAACAATGATGGACTCATCTCCTTTGCAGAGTATAAAGCTCAACCCTTTTCCTTCTTAAACCTAATACTCAATTTAAAGCTTAATGAACACTTTCAGGTACATCTTTTTTGTTACACTTCTAAGCATACCAGAGTCAAGCTTTTCAGTGGCATTCAAAATGTTTGATATTGACAACAATGGGTTAGACAATTTGTTCTCTATTTGTGTCGTGTTTCATGTTTTGTCATGTTTTTTTTTTGCCATTTTTGAACCATTTTGGTGTTGGAAATAGAGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGGTTGATGAGGAAACAAAACAGGCAAGGAGCTCATCATAGAGATGGGAGGAGGCTTGGGGTGAAAGCTTGCGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGGCTCTCTTCACCATGATAAGTTCGTTCAATTTTTAAGACAACTTCATGATGAGGTATGATTTCCCTTCGAATTTTGTATTCAAATCCGAGAACGGTCAAAATAAAGTTGGTTATATCGAACCAATGGGTTAGTTTCGATAGTGATCTATTGGGGTAAAATCTTGTATGTGAGATTCCCGCGTCGACTGGAAGCTTGTGTGTGGGATCCCACGTCGGCTGGAAGCTTGTGTGGAAGCTTGTATGTGGTGGGATCCCGCGTCGGCTGGAAGCTTGTATATATAAGATCCCACCTCGGCTTGAGAGGGGATCGAAACATTCTTCTCTATGACAGATGTTTTTAAAACCGTGAGACTGACGACGATACGTAACGGACAAAAACAGATAATATCTACTAGTGGTAGGTTTGGGTGGTTACAAATGGTATTAGAGCTAGACATCGGGCTGTGTGCTAGCAAGGACCTTGACCCCCAAGGGGTTGAATTGTAACATCTACTAGTGGTGGGTTTAGGTGGTTATAAATGGTATTAGAGCTAGACATTGGGCTGTGTGCTAGCAAGGACCTTGACCCCCAAGGATTGTAAGATCCCACATCGGTCGAAGAAGGGAACGAAACATTCTTTATAAGGGTGTGGAAATCTCTCCCCGGGTAGATAGTAAGATCCGACATCGATTGGAGAAGGGAACGAAACGTTCTTTAAAGGGTGTGGAAATCTCTCTCCAGCAAACGTTGGACAAAGATATCAAACGTTCACATGTTTAGGCTTATATCCTTAAACGTTCGAATTCCTTAGCTTAACAAAAGAAGAGCGGATTATGGCGTTCGTGATTGGAATACCTAAACTTTTGAATCGGGTGATTTTGCAGATACTTAAACTAGAGTTCTCACATTATGATTTCAAGTCACAAGGAAGCATATCAGCCAAAGACTTCGCACTCTCCATCGTTGCAGCAGCCGACATAAACCACATAGACAAGCTCCTCGATCGAGTCGAAGCATTAAACAAAGAACACCATTTCAAAAACATCCGAATCACATACGAAGAATTCAAACACTTCGCTGAGCTACGTAAGAAGCTGGAACCCTTCTCATTGGCCATTTTCAGCTATGGAAAAGTAAATGGAGAGCTCACAAAACAAGATTTCCAACGAGCCGCTTCCCAAGTAAGTATCACGTCACAATATTTGCTAAAACCACCGCATCTAAACGGTGGTGAAAGTGTAACTATTGTTATTACCGTCAAATATCTAGGGTTTAGGGTTTATTCCTCGCTATTGCAGTCAGGTTTATTTCTCGTTATTACAGTCAATATCTAGGGTTTAGGGTTTATTCCTCGTTATTACAGTCAAATATCTAGGGTTTAGGGTTTATTCCTCGTTATTACAGTCAATATCTAGGGTTTAGGGTTTATTCCTCGTTATTACAGTCAAATATCTAGGGTTTAGGGTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNTCGAGGACTCCTTCTCCTTCTTCCAACTCCGACTTGTATTGTTCATAGAGAAATCTCTGGGGATTTCTGGGTCTGGGTCGAAAAAGCAATGTCACTAGATCATTATCCCAATCCCAACCTAGTGTCTTCTACTTCTAATAAGTCATGAACTAGATCAGAATCATTTTCAACGAGTCTATAAGATATGATCCCATTTTTTTTTTCATTGGGTTTGGATAGAGACCAAAGGTCTTGAATGAACGATCGACGGATATTTGCCTGTAATATTCCCTAAACCCTAGATATTTATTACATATTTGACTGTAATAAAAGGTCAAATATGATATCCAATCTCATATTTGCCTGTAATAAACCCTAAACCCTAGATATTTGACTGTAATAAAAGGTCAAATATGATATTTAATTTCATGATTCTTGTGGCTTTTGGGAGTAAGGTCTGTGGAGTTTCGATCACGTCCAATGTCGTGGACATAATATTTCACATATTCGACGCGAATGGCGATGGCGACTTGAGCTCGGATGAGTTTGTGAGAGTTATCCAGAGAAGGGAAGTCAGTTCTTCGCAAGCGACTTTGGGCGTGTCGGGGTTTTTGTCGTGCTGGTTCAGCTGTGCGGCTCGATGCTCTTCTGCAAAGCTCTTTGTTCGCAGCTGAGTTCAAGAGACATCCATGGCAGCCTCGGCCTCCTGTAAATTTTTCAACA

mRNA sequence

ATAATTACAAAAGATGGGGTGCTTTTCGTATCCAACATTTTAACCTAATGGATCACGGTAAATTTAATCTTTTGTTCATATTTTGCGTTCGATCATTTCCAGATGATTACAGAAGACGAGTGTTTTTCAACTACGAAAAACGAATAAGAATCCAAAGCCCTCCCGAGAAGGTGTTCGAGTATTTTGCATCTATTCACACAGCGGACGGAGAAATTTTCATGACACCATCGGATCTAATGAGAGCGATTGTTCCTGTATTTCCTCCATCGGAGTCGAATCGAGTAAGAGAAGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTCTATTGTGTTCCTTCTAAATTCTTCATGCTTTTTGACACCAACAATGATGGACTCATCTCCTTTGCAGAGTACATCTTTTTTGTTACACTTCTAAGCATACCAGAGTCAAGCTTTTCAGTGGCATTCAAAATGTTTGATATTGACAACAATGGAGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGGTTGATGAGGAAACAAAACAGGCAAGGAGCTCATCATAGAGATGGGAGGAGGCTTGGGGTGAAAGCTTGCGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGGCTCTCTTCACCATGATAAGTTCGTTCAATTTTTAAGACAACTTCATGATGAGATACTTAAACTAGAGTTCTCACATTATGATTTCAAGTCACAAGGAAGCATATCAGCCAAAGACTTCGCACTCTCCATCGTTGCAGCAGCCGACATAAACCACATAGACAAGCTCCTCGATCGAGTCGAAGCATTAAACAAAGAACACCATTTCAAAAACATCCGAATCACATACGAAGAATTCAAACACTTCGCTGAGCTACGTAAGAAGCTGGAACCCTTCTCATTGGCCATTTTCAGCTATGGAAAAGTAAATGGAGAGCTCACAAAACAAGATTTCCAACGAGCCGCTTCCCAATTATCCAGAGAAGGGAAGTCAGTTCTTCGCAAGCGACTTTGGGCGTGTCGGGGTTTTTGTCGTGCTGGTTCAGCTGTGCGGCTCGATGCTCTTCTGCAAAGCTCTTTGTTCGCAGCTGAGTTCAAGAGACATCCATGGCAGCCTCGGCCTCCTGTAAATTTTTCAACA

Coding sequence (CDS)

ATGGATCACGGTAAATTTAATCTTTTGTTCATATTTTGCGTTCGATCATTTCCAGATGATTACAGAAGACGAGTGTTTTTCAACTACGAAAAACGAATAAGAATCCAAAGCCCTCCCGAGAAGGTGTTCGAGTATTTTGCATCTATTCACACAGCGGACGGAGAAATTTTCATGACACCATCGGATCTAATGAGAGCGATTGTTCCTGTATTTCCTCCATCGGAGTCGAATCGAGTAAGAGAAGGGTTTCTAAGAGGAGAAAGAAGAGTTCATGGAGAGCTCTATTGTGTTCCTTCTAAATTCTTCATGCTTTTTGACACCAACAATGATGGACTCATCTCCTTTGCAGAGTACATCTTTTTTGTTACACTTCTAAGCATACCAGAGTCAAGCTTTTCAGTGGCATTCAAAATGTTTGATATTGACAACAATGGAGAAATAGACAGGGAGGAGTTCAAGAAAGTGATGGGGTTGATGAGGAAACAAAACAGGCAAGGAGCTCATCATAGAGATGGGAGGAGGCTTGGGGTGAAAGCTTGCGTTGAAAATGGTGGTTTGGTTGAGTATTTCTTTGGTCAAGATGGAAATGGCTCTCTTCACCATGATAAGTTCGTTCAATTTTTAAGACAACTTCATGATGAGATACTTAAACTAGAGTTCTCACATTATGATTTCAAGTCACAAGGAAGCATATCAGCCAAAGACTTCGCACTCTCCATCGTTGCAGCAGCCGACATAAACCACATAGACAAGCTCCTCGATCGAGTCGAAGCATTAAACAAAGAACACCATTTCAAAAACATCCGAATCACATACGAAGAATTCAAACACTTCGCTGAGCTACGTAAGAAGCTGGAACCCTTCTCATTGGCCATTTTCAGCTATGGAAAAGTAAATGGAGAGCTCACAAAACAAGATTTCCAACGAGCCGCTTCCCAATTATCCAGAGAAGGGAAGTCAGTTCTTCGCAAGCGACTTTGGGCGTGTCGGGGTTTTTGTCGTGCTGGTTCAGCTGTGCGGCTCGATGCTCTTCTGCAAAGCTCTTTGTTCGCAGCTGAGTTCAAGAGACATCCATGGCAGCCTCGGCCTCCTGTAAATTTTTCAACA

Protein sequence

MDHGKFNLLFIFCVRSFPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSESNRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQLSREGKSVLRKRLWACRGFCRAGSAVRLDALLQSSLFAAEFKRHPWQPRPPVNFST
Homology
BLAST of Cp4.1LG07g10900 vs. ExPASy Swiss-Prot
Match: Q9SZ45 (Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 6.9e-116
Identity = 209/300 (69.67%), Postives = 251/300 (83.67%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F D YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T  GEI M P+DLMRAIVPVFPPSES
Sbjct: 134 FGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPSES 193

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           + VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF+VAF
Sbjct: 194 HLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAF 253

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG--VKACVENGGLVEYFFGQD 196
           KMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G  +   VE+GGLVEYFFG+D
Sbjct: 254 KMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKD 313

Query: 197 GNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLD 256
           G+  L HDKF +F++ L +E+L+LEF+HYD+K +GSISAKDFALS+VAAAD +H+ KLLD
Sbjct: 314 GSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLD 373

Query: 257 RVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 315
           RVE+L++  H +++RI+ +EFK F ELR KL PFSLA+F+YGK NG LT +DF+RAASQ+
Sbjct: 374 RVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAASQV 432

BLAST of Cp4.1LG07g10900 vs. ExPASy Swiss-Prot
Match: Q0IIL1 (Calcium uptake protein 1, mitochondrial OS=Bos taurus OX=9913 GN=MICU1 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 1.1e-36
Identity = 98/321 (30.53%), Postives = 162/321 (50.47%), Query Frame = 0

Query: 13  CVRSFPDD-------YRRRVFFNYEKRIRIQSPPEKVFEYFASIHT----ADGEIFMTPS 72
           C+  +P++       +R R    YE RIR  S P+K+F YFA++       + E+FMTP 
Sbjct: 93  CLEPYPEEKKKKRSGFRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEHGESEVFMTPQ 152

Query: 73  DLMRAIVPVFPPSESNRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAEYI 132
           D +R+I P     E   + +  ++    +++  E      +  + +     GLISF++YI
Sbjct: 153 DFVRSITPNEKQPEHLGLDQYTIKRFDGKKIAQEREKFADEGSIFYTLGECGLISFSDYI 212

Query: 133 FFVTLLSIPESSFSVAFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD----GRRL 192
           F  T+LS P+ +F +AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G  L
Sbjct: 213 FLTTVLSTPQRNFEIAFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRSTTGNTL 272

Query: 193 GVKACVENGGLVEYFFGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKD 252
               C     L  YFFG D  G L    F++F R+L  ++LKLEF  +D    G I+ + 
Sbjct: 273 KSGLC---SALTTYFFGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRITERQ 332

Query: 253 FALSIVAAADINHIDKLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSY 312
           F   ++A + +    K L  ++   K+H  +   +T++E ++F    K +     A+  Y
Sbjct: 333 FGGMLLAYSGVQ--SKKLTAMQKQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFY 392

Query: 313 GKVNGELTKQDFQRAASQLSR 317
                 L K   Q+ A  +++
Sbjct: 393 HMAGASLDKVTMQQVARTVAK 407

BLAST of Cp4.1LG07g10900 vs. ExPASy Swiss-Prot
Match: B1H2N3 (Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 PE=2 SV=2)

HSP 1 Score: 155.2 bits (391), Expect = 1.4e-36
Identity = 97/304 (31.91%), Postives = 158/304 (51.97%), Query Frame = 0

Query: 21  YRRRVFFNYEKRIRIQSPPEKVFEYFASI----HTADGEIFMTPSDLMRAIVPVFPPSES 80
           +R R    YE RIR  S P+K+F YFA++     + + E+FMTP D +R+I P     E+
Sbjct: 103 FRDRKVMEYENRIRAYSTPDKIFRYFATLKVIHESGESEVFMTPQDFVRSITPNEKQPEN 162

Query: 81  NRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSV 140
             + +  ++    +++  E      +  + +     GLISF++YIF  T+LS P+ +F +
Sbjct: 163 LGLDQFIIKRYDGKKISQEREKFADEDSIFYSLGECGLISFSDYIFLTTVLSTPQRNFEI 222

Query: 141 AFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG-VKACVENGGLVEYFFGQ 200
           AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G       +  L  YFFG 
Sbjct: 223 AFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRSTTGNTLKTGFSSALTTYFFGA 282

Query: 201 DGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLL 260
           D  G L    F++F R+L  ++LKLEF  +D    G I+ + F   ++A + +    KL 
Sbjct: 283 DLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGHITERQFGSMLLAYSGVQS-KKLT 342

Query: 261 DRVEALNKEHHFKNIR-ITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS 317
             ++ L K   FK+   +T+EE ++F    K +     A+  Y      L K   Q+ A 
Sbjct: 343 HMLKQLKK--RFKDAEGLTFEEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQVAR 402

BLAST of Cp4.1LG07g10900 vs. ExPASy Swiss-Prot
Match: Q9BPX6 (Calcium uptake protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=MICU1 PE=1 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.1e-36
Identity = 96/306 (31.37%), Postives = 156/306 (50.98%), Query Frame = 0

Query: 21  YRRRVFFNYEKRIRIQSPPEKVFEYFASI----HTADGEIFMTPSDLMRAIVPVFPPSES 80
           +R R    YE RIR  S P+K+F YFA++       + E+FMTP D +R+I P     E 
Sbjct: 106 FRDRKVMEYENRIRAYSTPDKIFRYFATLKVISEPGEAEVFMTPEDFVRSITPNEKQPEH 165

Query: 81  NRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSV 140
             + +  ++    +++  E      +  + +     GLISF++YIF  T+LS P+ +F +
Sbjct: 166 LGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEI 225

Query: 141 AFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD----GRRLGVKACVENGGLVEYF 200
           AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G  L    C     L  YF
Sbjct: 226 AFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLC---SALTTYF 285

Query: 201 FGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHID 260
           FG D  G L    F++F R+L  ++LKLEF  +D    G I+ + F   ++A + +    
Sbjct: 286 FGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRITERQFGGMLLAYSGVQ--S 345

Query: 261 KLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRA 317
           K L  ++   K+H  +   +T++E ++F    K +     A+  Y      L K   Q+ 
Sbjct: 346 KKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQV 405

BLAST of Cp4.1LG07g10900 vs. ExPASy Swiss-Prot
Match: Q4R518 (Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 PE=2 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 3.1e-36
Identity = 96/306 (31.37%), Postives = 156/306 (50.98%), Query Frame = 0

Query: 21  YRRRVFFNYEKRIRIQSPPEKVFEYFASI----HTADGEIFMTPSDLMRAIVPVFPPSES 80
           +R R    YE RIR  S P+K+F YFA++       + E+FMTP D +R+I P     E 
Sbjct: 106 FRDRKVMEYENRIRAYSTPDKIFRYFATLKVINEPGEAEVFMTPEDFVRSITPNEKQPEH 165

Query: 81  NRVREGFLR--GERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSV 140
             + +  ++    +++  E      +  + +     GLISF++YIF  T+LS P+ +F +
Sbjct: 166 LGLDQYIIKRFDGKKISQEREKFADEGSIFYTLGECGLISFSDYIFLTTVLSTPQRNFEI 225

Query: 141 AFKMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRD----GRRLGVKACVENGGLVEYF 200
           AFKMFD++ +GE+D EEF++V  ++R Q   G  HRD    G  L    C     L  YF
Sbjct: 226 AFKMFDLNGDGEVDMEEFEQVQSIIRSQTSMGMRHRDRPTTGNTLKSGLC---SALTTYF 285

Query: 201 FGQDGNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHID 260
           FG D  G L    F++F R+L  ++LKLEF  +D    G I+ + F   ++A + +    
Sbjct: 286 FGADLKGKLTIKNFLEFQRKLQHDVLKLEFERHD-PVDGRITERQFGGMLLAYSGVQ--S 345

Query: 261 KLLDRVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRA 317
           K L  ++   K+H  +   +T++E ++F    K +     A+  Y      L K   Q+ 
Sbjct: 346 KKLTAMQRQLKKHFKEGKGLTFQEVENFFTFLKNINDVDTALSFYHMAGASLDKVTMQQV 405

BLAST of Cp4.1LG07g10900 vs. NCBI nr
Match: XP_023522354.1 (calcium uptake protein, mitochondrial-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 594 bits (1531), Expect = 6.26e-211
Identity = 295/298 (98.99%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 108 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSES 167

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 168 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 227

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 228 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 287

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV
Sbjct: 288 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 347

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQ+
Sbjct: 348 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQV 405

BLAST of Cp4.1LG07g10900 vs. NCBI nr
Match: XP_023538495.1 (calcium uptake protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_023538497.1 calcium uptake protein, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 595 bits (1535), Expect = 1.21e-210
Identity = 296/298 (99.33%), Postives = 297/298 (99.66%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 103 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 162

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 163 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 222

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 223 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 282

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV
Sbjct: 283 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 342

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQ+
Sbjct: 343 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQV 400

BLAST of Cp4.1LG07g10900 vs. NCBI nr
Match: XP_022965722.1 (calcium uptake protein, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 593 bits (1528), Expect = 1.67e-209
Identity = 294/298 (98.66%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 108 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSES 167

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 168 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 227

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 228 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 287

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVA+ADINHIDKLLDRV
Sbjct: 288 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRV 347

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQ+
Sbjct: 348 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQV 405

BLAST of Cp4.1LG07g10900 vs. NCBI nr
Match: XP_022937835.1 (calcium uptake protein, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 590 bits (1520), Expect = 2.76e-208
Identity = 292/298 (97.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 108 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSES 167

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPE+SFSVAF
Sbjct: 168 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAF 227

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 228 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 287

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVA+ADINHIDKLLDRV
Sbjct: 288 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRV 347

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS +
Sbjct: 348 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHV 405

BLAST of Cp4.1LG07g10900 vs. NCBI nr
Match: KAG7021189.1 (Calcium uptake protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588 bits (1516), Expect = 1.12e-207
Identity = 291/298 (97.65%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 108 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSES 167

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPE+SFSVAF
Sbjct: 168 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAF 227

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 228 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 287

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKS+GSISAKDFALSIVA+ADINHIDKLLDRV
Sbjct: 288 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSRGSISAKDFALSIVASADINHIDKLLDRV 347

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS +
Sbjct: 348 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHV 405

BLAST of Cp4.1LG07g10900 vs. ExPASy TrEMBL
Match: A0A6J1HL37 (calcium uptake protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111465533 PE=4 SV=1)

HSP 1 Score: 593 bits (1528), Expect = 8.10e-210
Identity = 294/298 (98.66%), Postives = 296/298 (99.33%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 108 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSES 167

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 168 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 227

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 228 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 287

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVA+ADINHIDKLLDRV
Sbjct: 288 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRV 347

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQ+
Sbjct: 348 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQV 405

BLAST of Cp4.1LG07g10900 vs. ExPASy TrEMBL
Match: A0A6J1FHX4 (calcium uptake protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111444105 PE=4 SV=1)

HSP 1 Score: 590 bits (1520), Expect = 1.33e-208
Identity = 292/298 (97.99%), Postives = 295/298 (98.99%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT DGEIFMTPSDLMRAIVPVFPPSES
Sbjct: 108 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTVDGEIFMTPSDLMRAIVPVFPPSES 167

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPE+SFSVAF
Sbjct: 168 NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPETSFSVAF 227

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN
Sbjct: 228 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 287

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVA+ADINHIDKLLDRV
Sbjct: 288 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVASADINHIDKLLDRV 347

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAAS +
Sbjct: 348 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASHV 405

BLAST of Cp4.1LG07g10900 vs. ExPASy TrEMBL
Match: A0A1S3C3I8 (calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 552 bits (1422), Expect = 7.45e-193
Identity = 276/299 (92.31%), Postives = 284/299 (94.98%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT +GEI+M PSDLMRAIVPVFPPSES
Sbjct: 137 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSES 196

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 197 NRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 256

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR G+K  VENGGLVEYFFG+DG 
Sbjct: 257 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVENGGLVEYFFGEDGK 316

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSIS KDFALSIVA+ADINHIDKLLDRV
Sbjct: 317 GSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHIDKLLDRV 376

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQLS 315
           EALNKE HFKNIRITYEEFK FAELRKKLE FSLAIFSYGKVNGELTKQDFQRAASQ+S
Sbjct: 377 EALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASQVS 435

BLAST of Cp4.1LG07g10900 vs. ExPASy TrEMBL
Match: A0A5A7VDG3 (Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold285G001000 PE=4 SV=1)

HSP 1 Score: 550 bits (1418), Expect = 1.18e-192
Identity = 275/298 (92.28%), Postives = 283/298 (94.97%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT +GEI+M PSDLMRAIVPVFPPSES
Sbjct: 137 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSES 196

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 197 NRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 256

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR G+K  VENGGLVEYFFG+DG 
Sbjct: 257 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVENGGLVEYFFGEDGK 316

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSIS KDFALSIVA+ADINHIDKLLDRV
Sbjct: 317 GSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHIDKLLDRV 376

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKE HFKNIRITYEEFK FAELRKKLE FSLAIFSYGKVNGELTKQDFQRAASQ+
Sbjct: 377 EALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASQV 434

BLAST of Cp4.1LG07g10900 vs. ExPASy TrEMBL
Match: A0A1S3C4U2 (calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496476 PE=4 SV=1)

HSP 1 Score: 550 bits (1418), Expect = 1.18e-192
Identity = 275/298 (92.28%), Postives = 283/298 (94.97%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F DDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHT +GEI+M PSDLMRAIVPVFPPSES
Sbjct: 137 FGDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTPEGEIYMRPSDLMRAIVPVFPPSES 196

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           NRVREGFLRGERR+ GEL C PSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF
Sbjct: 197 NRVREGFLRGERRISGELCCAPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 256

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLGVKACVENGGLVEYFFGQDGN 196
           KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRR G+K  VENGGLVEYFFG+DG 
Sbjct: 257 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRFGMKVSVENGGLVEYFFGEDGK 316

Query: 197 GSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLDRV 256
           GSLHHDKFVQFLRQLH+EIL LEFSHYDFKSQGSIS KDFALSIVA+ADINHIDKLLDRV
Sbjct: 317 GSLHHDKFVQFLRQLHEEILILEFSHYDFKSQGSISVKDFALSIVASADINHIDKLLDRV 376

Query: 257 EALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 314
           EALNKE HFKNIRITYEEFK FAELRKKLE FSLAIFSYGKVNGELTKQDFQRAASQ+
Sbjct: 377 EALNKEPHFKNIRITYEEFKDFAELRKKLESFSLAIFSYGKVNGELTKQDFQRAASQV 434

BLAST of Cp4.1LG07g10900 vs. TAIR 10
Match: AT4G32060.2 (calcium-binding EF hand family protein )

HSP 1 Score: 419.9 bits (1078), Expect = 2.2e-117
Identity = 212/305 (69.51%), Postives = 254/305 (83.28%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F D YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T  GEI M P+DLMRAIVPVFPPSES
Sbjct: 134 FGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPSES 193

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           + VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF+VAF
Sbjct: 194 HLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAF 253

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG--VKACVENGGLVEYFFGQD 196
           KMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G  +   VE+GGLVEYFFG+D
Sbjct: 254 KMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKD 313

Query: 197 GNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLD 256
           G+  L HDKF +F++ L +E+L+LEF+HYD+K +GSISAKDFALS+VAAAD +H+ KLLD
Sbjct: 314 GSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLD 373

Query: 257 RVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 316
           RVE+L++  H +++RI+ +EFK F ELR KL PFSLA+F+YGK NG LT +DF+RAASQ 
Sbjct: 374 RVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAASQ- 433

Query: 317 SREGK 320
            REG+
Sbjct: 434 -REGR 435

BLAST of Cp4.1LG07g10900 vs. TAIR 10
Match: AT4G32060.1 (calcium-binding EF hand family protein )

HSP 1 Score: 418.7 bits (1075), Expect = 4.9e-117
Identity = 209/300 (69.67%), Postives = 251/300 (83.67%), Query Frame = 0

Query: 17  FPDDYRRRVFFNYEKRIRIQSPPEKVFEYFASIHTADGEIFMTPSDLMRAIVPVFPPSES 76
           F D YRR++FFNYEKR+R+QSPPEKVFEYFAS+ T  GEI M P+DLMRAIVPVFPPSES
Sbjct: 134 FGDAYRRKIFFNYEKRLRLQSPPEKVFEYFASVRTDKGEILMKPADLMRAIVPVFPPSES 193

Query: 77  NRVREGFLRGERRVHGELYCVPSKFFMLFDTNNDGLISFAEYIFFVTLLSIPESSFSVAF 136
           + VREG+L GER   GEL C PS+FFMLFD +NDGLISF EYIFFVTLLSIPESSF+VAF
Sbjct: 194 HLVREGYLTGERN-PGELRCSPSEFFMLFDVDNDGLISFKEYIFFVTLLSIPESSFAVAF 253

Query: 137 KMFDIDNNGEIDREEFKKVMGLMRKQNRQGAHHRDGRRLG--VKACVENGGLVEYFFGQD 196
           KMFD DNNGEID+EEFK VM LMR Q+RQG  HRDG R G  +   VE+GGLVEYFFG+D
Sbjct: 254 KMFDTDNNGEIDKEEFKTVMSLMRSQHRQGVGHRDGLRTGLHMTGSVEDGGLVEYFFGKD 313

Query: 197 GNGSLHHDKFVQFLRQLHDEILKLEFSHYDFKSQGSISAKDFALSIVAAADINHIDKLLD 256
           G+  L HDKF +F++ L +E+L+LEF+HYD+K +GSISAKDFALS+VAAAD +H+ KLLD
Sbjct: 314 GSQKLKHDKFTKFMKDLTEEMLRLEFAHYDYKRRGSISAKDFALSMVAAADASHLSKLLD 373

Query: 257 RVEALNKEHHFKNIRITYEEFKHFAELRKKLEPFSLAIFSYGKVNGELTKQDFQRAASQL 315
           RVE+L++  H +++RI+ +EFK F ELR KL PFSLA+F+YGK NG LT +DF+RAASQ+
Sbjct: 374 RVESLSEHPHLRDMRISLKEFKQFDELRSKLGPFSLALFAYGKANGLLTMKDFKRAASQV 432

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZ456.9e-11669.67Calcium uptake protein, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MICU PE... [more]
Q0IIL11.1e-3630.53Calcium uptake protein 1, mitochondrial OS=Bos taurus OX=9913 GN=MICU1 PE=2 SV=1[more]
B1H2N31.4e-3631.91Calcium uptake protein 1, mitochondrial OS=Xenopus tropicalis OX=8364 GN=micu1 P... [more]
Q9BPX63.1e-3631.37Calcium uptake protein 1, mitochondrial OS=Homo sapiens OX=9606 GN=MICU1 PE=1 SV... [more]
Q4R5183.1e-3631.37Calcium uptake protein 1, mitochondrial OS=Macaca fascicularis OX=9541 GN=MICU1 ... [more]
Match NameE-valueIdentityDescription
XP_023522354.16.26e-21198.99calcium uptake protein, mitochondrial-like, partial [Cucurbita pepo subsp. pepo][more]
XP_023538495.11.21e-21099.33calcium uptake protein, mitochondrial-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022965722.11.67e-20998.66calcium uptake protein, mitochondrial-like [Cucurbita maxima][more]
XP_022937835.12.76e-20897.99calcium uptake protein, mitochondrial-like [Cucurbita moschata][more]
KAG7021189.11.12e-20797.65Calcium uptake protein, mitochondrial [Cucurbita argyrosperma subsp. argyrosperm... [more]
Match NameE-valueIdentityDescription
A0A6J1HL378.10e-21098.66calcium uptake protein, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1FHX41.33e-20897.99calcium uptake protein, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A1S3C3I87.45e-19392.31calcium uptake protein 1, mitochondrial-like isoform X1 OS=Cucumis melo OX=3656 ... [more]
A0A5A7VDG31.18e-19292.28Calcium uptake protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3C4U21.18e-19292.28calcium uptake protein 1, mitochondrial-like isoform X2 OS=Cucumis melo OX=3656 ... [more]
Match NameE-valueIdentityDescription
AT4G32060.22.2e-11769.51calcium-binding EF hand family protein [more]
AT4G32060.14.9e-11769.67calcium-binding EF hand family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 131..159
e-value: 2.6E-5
score: 33.7
coord: 97..125
e-value: 0.032
score: 23.4
IPR002048EF-hand domainPFAMPF13833EF-hand_8coord: 102..123
e-value: 8.4E-4
score: 19.1
IPR002048EF-hand domainPFAMPF00036EF-hand_1coord: 135..157
e-value: 6.0E-7
score: 28.6
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 127..162
score: 13.118635
IPR002048EF-hand domainCDDcd00051EFhcoord: 100..156
e-value: 2.09061E-11
score: 56.7873
NoneNo IPR availableGENE3D1.10.238.10coord: 36..179
e-value: 1.9E-15
score: 58.8
NoneNo IPR availableGENE3D1.10.238.10coord: 186..293
e-value: 5.4E-7
score: 31.2
NoneNo IPR availablePANTHERPTHR12294:SF23CALCIUM UPTAKE PROTEIN, MITOCHONDRIALcoord: 17..314
NoneNo IPR availableCDDcd15900EFh_MICUcoord: 132..259
e-value: 4.70368E-29
score: 108.085
IPR039800Calcium uptake protein 1/2/3PANTHERPTHR12294EF HAND DOMAIN FAMILY A1,A2-RELATEDcoord: 17..314
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 140..152
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 100..237

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG07g10900.1Cp4.1LG07g10900.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0036444 calcium import into the mitochondrion
biological_process GO:0051560 mitochondrial calcium ion homeostasis
biological_process GO:0006851 mitochondrial calcium ion transmembrane transport
cellular_component GO:1990246 uniplex complex
molecular_function GO:0005509 calcium ion binding