Cp4.1LG07g09530 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG07g09530
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase
Location	Cp4.1LG07: 8627458 .. 8628346 (-)
RNA-Seq Expression	Cp4.1LG07g09530
Synteny	Cp4.1LG07g09530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGGTTGAGGGAAGTGAAGATGGGGGAAGCAGTTGGCATCTTCTCGATGAACAAACAAATCAGATGTTTGACAACCGTTTTCAACGTAGGAGTTTCTTTGTTGCCAAAACTGGTTACTTATCAAATGCTTTCAGGTAAATCTTCGGGAAAAATTCACTTTTTACTCGCTTTCTATCAATCTTCTCAATGAACAGATAAATTAGATGTTTGATGTTTGAGAATACTTTTGTTAATGAATTTTCAAACCGGTTTGAGAATACTTTTTGTTAATAAATTTTCAAACCAAATCAAAATAAAGGGCCGAAATTGCTCTATTCCAAGTTTAATATCTAGGTGTAAATTGATGAAATTGAAAGTATAGGATAAAAATTGAAAGTATAGGATAAAAATTGATACAACTGAACAAGTTTATGAATAGATATGAATTTTTTCCTTCTGTAAATTCTATTAAAACTTATAATTATAAGATTGAAGCACGATTACTTGCACTTCTCTAACTTGTAAAACATGGGTTCAGGTTTACATTCTTGACCGTTCGTGACATCGACTCGACATCAAAGTTCCAAATTGGTAGCATTGATTTGTATTCTCGCAAGTTTTAGCTCAATAAAAATGAGAGCTTCGAGATCCGTATGGTTCTCTTCAAGTTGGCCGAACAGAGTTCATAAGAAATCATATGTGGTCGAGATGATTTTGAAGGTTTGCTGCAGTGTGTAAAGCGGATCTTCAATAATCTAAATTGTGGATCAGCAAGTATGTCTGTTTGGTGAGCTAATTTATGGGTCAAAAATGAATAAAAAATGAATGTAACAGAAGCAACTAATTCTATTCCCCCTTTTTTAAACAAAAATTATGGTATCAAAATTTTGTCAAATAATCATTTAGGTTC

mRNA sequence

TTGGTTGAGGGAAGTGAAGATGGGGGAAGCAGTTGGCATCTTCTCGATGAACAAACAAATCAGATGTTTGACAACCGTTTTCAACGTAGGAGTTTCTTTGTTGCCAAAACTGGTTACTTATCAAATGCTTTCAGGTTTACATTCTTGACCGTTCGTGACATCGACTCGACATCAAAGTTCCAAATTGGTAGCATTGATTTGTATTCTCGCAAGTTTTAGCTCAATAAAAATGAGAGCTTCGAGATCCGTATGGTTCTCTTCAAGTTGGCCGAACAGAGTTCATAAGAAATCATATGTGGTCGAGATGATTTTGAAGGTTTGCTGCAGTGTGTAAAGCGGATCTTCAATAATCTAAATTGTGGATCAGCAAGTATGTCTGTTTGGTGAGCTAATTTATGGGTCAAAAATGAATAAAAAATGAATGTAACAGAAGCAACTAATTCTATTCCCCCTTTTTTAAACAAAAATTATGGTATCAAAATTTTGTCAAATAATCATTTAGGTTC

Coding sequence (CDS)

TTGGTTGAGGGAAGTGAAGATGGGGGAAGCAGTTGGCATCTTCTCGATGAACAAACAAATCAGATGTTTGACAACCGTTTTCAACGTAGGAGTTTCTTTGTTGCCAAAACTGGTTACTTATCAAATGCTTTCAGGTTTACATTCTTGACCGTTCGTGACATCGACTCGACATCAAAGTTCCAAATTGGTAGCATTGATTTGTATTCTCGCAAGTTTTAG

Protein sequence

LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKFQIGSIDLYSRKF

Homology

[Close]

BLAST of Cp4.1LG07g09530 vs. ExPASy Swiss-Prot
Match: Q9FGY9 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Arabidopsis thaliana OX=3702 GN=PNG1 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 6.4e-18
Identity = 39/68 (57.35%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           ++EGS DGGS+W +LD+QT+Q+F+ RFQR+S+ +   G+ +N FRF FL+VRD++STS+ 
Sbjct: 650 ILEGSNDGGSTWCVLDKQTSQVFEERFQRKSYKITTPGFQANLFRFRFLSVRDVNSTSRL 709

Query: 61  QIGSIDLY 69
           Q+GSIDLY
Sbjct: 710 QLGSIDLY 717

[Close]

BLAST of Cp4.1LG07g09530 vs. NCBI nr
Match: XP_023537983.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147 bits (371), Expect = 1.03e-40
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF
Sbjct: 333 LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 392

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLYSRKF
Sbjct: 393 QIGSIDLYSRKF 404

[Close]

BLAST of Cp4.1LG07g09530 vs. NCBI nr
Match: XP_023521128.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 147 bits (371), Expect = 1.62e-40
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF
Sbjct: 358 LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 417

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLYSRKF
Sbjct: 418 QIGSIDLYSRKF 429

[Close]

BLAST of Cp4.1LG07g09530 vs. NCBI nr
Match: XP_022937666.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucurbita moschata])

HSP 1 Score: 147 bits (371), Expect = 1.69e-39
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF
Sbjct: 541 LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 600

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLYSRKF
Sbjct: 601 QIGSIDLYSRKF 612

[Close]

BLAST of Cp4.1LG07g09530 vs. NCBI nr
Match: KAG6586304.1 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 142 bits (357), Expect = 3.63e-37
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 1    LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
            LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF
Sbjct: 951  LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 1010

Query: 61   QIGSIDLYSRK 71
            QIGSIDLYS K
Sbjct: 1011 QIGSIDLYSCK 1021

[Close]

BLAST of Cp4.1LG07g09530 vs. NCBI nr
Match: XP_022965640.1 (peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucurbita maxima])

HSP 1 Score: 140 bits (354), Expect = 4.25e-37
Identity = 69/72 (95.83%), Postives = 71/72 (98.61%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           LVEGSEDGGSSWHLLDEQTNQ+FDNR QRRSFFVAKTG+LSNAFRFTFLTVRDIDSTSKF
Sbjct: 541 LVEGSEDGGSSWHLLDEQTNQIFDNRSQRRSFFVAKTGHLSNAFRFTFLTVRDIDSTSKF 600

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLYSRKF
Sbjct: 601 QIGSIDLYSRKF 612

[Close]

BLAST of Cp4.1LG07g09530 vs. ExPASy TrEMBL
Match: A0A6J1FB07 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucurbita moschata OX=3662 GN=LOC111444002 PE=4 SV=1)

HSP 1 Score: 147 bits (371), Expect = 8.20e-40
Identity = 72/72 (100.00%), Postives = 72/72 (100.00%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF
Sbjct: 541 LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 600

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLYSRKF
Sbjct: 601 QIGSIDLYSRKF 612

[Close]

BLAST of Cp4.1LG07g09530 vs. ExPASy TrEMBL
Match: A0A6J1HP96 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucurbita maxima OX=3661 GN=LOC111465478 PE=4 SV=1)

HSP 1 Score: 140 bits (354), Expect = 2.06e-37
Identity = 69/72 (95.83%), Postives = 71/72 (98.61%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           LVEGSEDGGSSWHLLDEQTNQ+FDNR QRRSFFVAKTG+LSNAFRFTFLTVRDIDSTSKF
Sbjct: 541 LVEGSEDGGSSWHLLDEQTNQIFDNRSQRRSFFVAKTGHLSNAFRFTFLTVRDIDSTSKF 600

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLYSRKF
Sbjct: 601 QIGSIDLYSRKF 612

[Close]

BLAST of Cp4.1LG07g09530 vs. ExPASy TrEMBL
Match: A0A6J1CPB8 (peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase OS=Momordica charantia OX=3673 GN=LOC111013072 PE=4 SV=1)

HSP 1 Score: 119 bits (299), Expect = 9.29e-30
Identity = 56/69 (81.16%), Postives = 64/69 (92.75%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           +VEGSEDGG+SWHLLDEQTNQMF+NRFQRRSFF+ +TG+LSNAFRF FLTV DI +TSK 
Sbjct: 540 IVEGSEDGGTSWHLLDEQTNQMFENRFQRRSFFITETGHLSNAFRFRFLTVCDIKATSKL 599

Query: 61  QIGSIDLYS 69
           QIGSIDLY+
Sbjct: 600 QIGSIDLYA 608

[Close]

BLAST of Cp4.1LG07g09530 vs. ExPASy TrEMBL
Match: A0A5D3D4F2 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001350 PE=4 SV=1)

HSP 1 Score: 119 bits (297), Expect = 1.70e-29
Identity = 56/72 (77.78%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           +VEGSEDGG+SWHL+DEQTNQ+FDNRFQRRSFFV KTG LSN FRF FL VRD +STS+ 
Sbjct: 523 IVEGSEDGGNSWHLIDEQTNQIFDNRFQRRSFFVTKTGLLSNTFRFRFLAVRDGESTSRL 582

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLY+  F
Sbjct: 583 QIGSIDLYAPSF 594

[Close]

BLAST of Cp4.1LG07g09530 vs. ExPASy TrEMBL
Match: A0A5A7VB18 (Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold329G001280 PE=4 SV=1)

HSP 1 Score: 119 bits (297), Expect = 1.79e-29
Identity = 56/72 (77.78%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           +VEGSEDGG+SWHL+DEQTNQ+FDNRFQRRSFFV KTG LSN FRF FL VRD +STS+ 
Sbjct: 540 IVEGSEDGGNSWHLIDEQTNQIFDNRFQRRSFFVTKTGLLSNTFRFRFLAVRDGESTSRL 599

Query: 61  QIGSIDLYSRKF 72
           QIGSIDLY+  F
Sbjct: 600 QIGSIDLYAPSF 611

[Close]

BLAST of Cp4.1LG07g09530 vs. TAIR 10
Match: AT5G49570.1 (peptide-N-glycanase 1 )

HSP 1 Score: 90.9 bits (224), Expect = 4.5e-19
Identity = 39/68 (57.35%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 1   LVEGSEDGGSSWHLLDEQTNQMFDNRFQRRSFFVAKTGYLSNAFRFTFLTVRDIDSTSKF 60
           ++EGS DGGS+W +LD+QT+Q+F+ RFQR+S+ +   G+ +N FRF FL+VRD++STS+ 
Sbjct: 650 ILEGSNDGGSTWCVLDKQTSQVFEERFQRKSYKITTPGFQANLFRFRFLSVRDVNSTSRL 709

Query: 61  QIGSIDLY 69
           Q+GSIDLY
Sbjct: 710 QLGSIDLY 717

The following BLAST results are available for this feature:

BLAST of Cp4.1LG07g09530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FGY9	6.4e-18	57.35	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Arabidopsis thali...	[more]

BLAST of Cp4.1LG07g09530 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023537983.1	1.03e-40	100.00	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase-like [Cucurbita pepo...	[more]
XP_023521128.1	1.62e-40	100.00	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucurbita pepo subs...	[more]
XP_022937666.1	1.69e-39	100.00	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucurbita moschata]	[more]
KAG6586304.1	3.63e-37	98.59	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase, partial [Cucurbita ...	[more]
XP_022965640.1	4.25e-37	95.83	peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase [Cucurbita maxima]	[more]

BLAST of Cp4.1LG07g09530 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FB07	8.20e-40	100.00	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucurbita moschat...	[more]
A0A6J1HP96	2.06e-37	95.83	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucurbita maxima ...	[more]
A0A6J1CPB8	9.29e-30	81.16	peptide-N(4)-(N-acetyl-beta- glucosaminyl)asparagine amidase OS=Momordica charan...	[more]
A0A5D3D4F2	1.70e-29	77.78	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var....	[more]
A0A5A7VB18	1.79e-29	77.78	Peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase OS=Cucumis melo var....	[more]

BLAST of Cp4.1LG07g09530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G49570.1	4.5e-19	57.35	peptide-N-glycanase 1	[more]

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG07g09530.1	Cp4.1LG07g09530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006516	glycoprotein catabolic process
biological_process	GO:0006517	protein deglycosylation
biological_process	GO:0006515	protein quality control for misfolded or incompletely synthesized proteins
biological_process	GO:0010188	response to microbial phytotoxin
biological_process	GO:0010193	response to ozone
biological_process	GO:0009751	response to salicylic acid
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0000224	peptide-N4-(N-acetyl-beta-glucosaminyl)asparagine amidase activity